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Giovanni Strambini

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TOP 3
Giovanni B Strambini; Margherita Gonnelli
Influence of denaturants on native-state structural fluctuations in azurin probed by molecular size-dependent quenching of Trp phosphorescence.
Giovanni B Strambini; Margherita Gonnelli
Amplitude spectrum of structural fluctuations in proteins from the internal diffusion of solutes of increasing molecular size: a Trp phosphorescence quenching study.
Giovanni B Strambini; Margherita Gonnelli
Acrylonitrile quenching of trp phosphorescence in proteins: a probe of the internal flexibility of the globular fold.

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All Publications

2011: Giovanni B Strambini; Margherita Gonnelli
Influence of denaturants on native-state structural fluctuations in azurin probed by molecular size-dependent quenching of Trp phosphorescence.
The journal of physical chemistry. B 2011;115(46):13755-64.
2011: Giovanni B Strambini; Margherita Gonnelli
Amplitude spectrum of structural fluctuations in proteins from the internal diffusion of solutes of increasing molecular size: a Trp phosphorescence quenching study.
Biochemistry 2011;50(6):970-80.
2010: Giovanni B Strambini; Margherita Gonnelli
Acrylonitrile quenching of trp phosphorescence in proteins: a probe of the internal flexibility of the globular fold.
Biophysical journal 2010;99(3):944-52.
2010: Giovanni B Strambini; Margherita Gonnelli
Protein phosphorescence quenching: distinction between quencher penetration and external quenching mechanisms.
The journal of physical chemistry. B 2010;114(29):9691-7.
2010: Giovanni B Strambini; Margherita Gonnelli
Fluorescence quenching of buried Trp residues by acrylamide does not require penetration of the protein fold.
The journal of physical chemistry. B 2010;114(2):1089-93.
2009: Giovanni B Strambini; Margherita Gonnelli
Acrylamide quenching of Trp phosphorescence in liver alcohol dehydrogenase: evidence of gated quencher penetration.
Biochemistry 2009;48(31):7482-91.
2009: Margherita Gonnelli; Giovanni B Strambini
No effect of covalently linked poly(ethylene glycol) chains on protein internal dynamics.
Biochimica et biophysica acta 2009;1794(3):569-76.
2008: Bruce A Kerwin; Kenneth H Aoki; Margherita Gonelli; Giovanni B Strambini
Differentiation of the local structure around tryptophan 51 and 64 in recombinant human erythropoietin by tryptophan phosphorescence.
Photochemistry and photobiology 2008;84(5):1172-81.
2008: Giovanni B Strambini; Margherita Gonnelli
Specific anions effects of on the stability of azurin in ice.
The journal of physical chemistry. B 2008;112(33):10255-63.
2008: Sabato D'Auria; Maria Staiano; Antonio Varriale; Margherita Gonnelli; Anna Marabotti; Mose' Rossi; Giovanni B Strambini
The differences in the microenvironment of the two tryptophan residues of the glutamine-binding protein from Escherichia coli shed light on the binding properties and the structural dynamics of the protein.
Proteins 2008;71(2):743-50.
2008: Giovanni B Strambini; Ettore Balestreri; Alvaro Galli; Margherita Gonnelli
Effects of sugars and polyols on the stability of azurin in ice.
The journal of physical chemistry. B 2008;112(14):4372-80.
2008: Giovanni B Strambini; Margherita Gonnelli
Singular efficacy of trimethylamine N-oxide to counter protein destabilization in ice.
Biochemistry 2008;47(11):3322-31.
2008: Sabato D'Auria; Maria Staiano; Antonio Varriale; Margherita Gonnelli; Anna Marabotti; Mose' Rossi; Giovanni B Strambini
The tryptophan phosphorescence of porcine and mutant bovine odorant-binding proteins: a probe for the local protein structure and dynamics.
Journal of proteome research 2008;7(3):1151-8.
2007: Sabato D'Auria; Antonio Varriale; Margherita Gonnelli; Michele Saviano; Maria Staiano; Mose' Rossi; Giovanni B Strambini
Tryptophan phosphorescence studies of the D-galactose/D-glucose-binding protein from Escherichia coli provide a molecular portrait with structural and dynamics features of the protein.
Journal of proteome research 2007;6(4):1306-12.
2007: Giovanni B Strambini; Margherita Gonnelli
Protein stability in ice.
Biophysical journal 2007;92(6):2131-8.
2006: Edi Gabellieri; Giovanni B Strambini; Dzmitry G. Shcharbin; Barbara Klajnert; Maria Bryszewska
Dendrimer-protein interactions studied by tryptophan room temperature phosphorescence.
Biochimica et biophysica acta 2006;1764(11):1750-6.
2006: Edi Gabellieri; Giovanni B Strambini
ANS fluorescence detects widespread perturbations of protein tertiary structure in ice.
Biophysical journal 2006;90(9):3239-45.
2005: Gertjan Veldhuis; Edi Gabellieri; Erwin P P Vos; Bert Poolman; Giovanni B Strambini; Jaap Broos
Substrate-induced conformational changes in the membrane-embedded IIC(mtl)-domain of the mannitol permease from Escherichia coli, EnzymeII(mtl), probed by tryptophan phosphorescence spectroscopy.
The Journal of biological chemistry 2005;280(42):35148-56.
2005: Patrizia Cioni; Emilia Bramanti; Giovanni B Strambini
Effects of sucrose on the internal dynamics of azurin.
Biophysical journal 2005;88(6):4213-22.
2005: Margherita Gonnelli; Giovanni B Strambini
Intramolecular quenching of tryptophan phosphorescence in short peptides and proteins.
Photochemistry and photobiology 2005;81(3):614-22.
2005: L Maragliano; Mattia Falconi; Alessandro Sergi; Patrizia Cioni; S Castelli; A Lania; Maria Elena Stroppolo; Giovanni B Strambini; M Ferrario; Alessandro Desideri
Experimental and simulative dissociation of dimeric Cu,Zn superoxide dismutase doubly mutated at the intersubunit surface.
Biophysical journal 2005;88(4):2875-82.
2004: Giovanni B Strambini; Bruce A Kerwin; Bruce D Mason; Margherita Gonnelli
The triplet-state lifetime of indole derivatives in aqueous solution.
Photochemistry and photobiology 2004;80(3):462-70.
2004: Emöke Bódis; Giovanni B Strambini; Margherita Gonnelli; András Málnási-Csizmadia; Béla Somogyi
Characterization of f-actin tryptophan phosphorescence in the presence and absence of tryptophan-free myosin motor domain.
Biophysical journal 2004;87(2):1146-54.
2004: Patrizia Cioni; Ellen de Waal; Gerard W Canters; Giovanni B Strambini
Effects of cavity-forming mutations on the internal dynamics of azurin.
Biophysical journal 2004;86(2):1149-59.
2003: Jaap Broos; Edi Gabellieri; Gijs I van Boxel; J Baz Jackson; Giovanni B Strambini
Tryptophan phosphorescence spectroscopy reveals that a domain in the NAD(H)-binding component (dI) of transhydrogenase from Rhodospirillum rubrum has an extremely rigid and conformationally homogeneous protein core.
The Journal of biological chemistry 2003;278(48):47578-84.
2003: Edi Gabellieri; Giovanni B Strambini
Perturbation of protein tertiary structure in frozen solutions revealed by 1-anilino-8-naphthalene sulfonate fluorescence.
Biophysical journal 2003;85(5):3214-20.
2003: Jaap Broos; Edi Gabellieri; Esther Biemans-Oldehinkel; Giovanni B Strambini
Efficient biosynthetic incorporation of tryptophan and indole analogs in an integral membrane protein.
Protein science : a publication of the Protein Society 2003;12(9):1991-2000.
2003: Margherita Gonnelli; Giovanni B Strambini
Structure and dynamics of proteins encapsulated in silica hydrogels by Trp phosphorescence.
Biophysical chemistry 2003;104(1):155-69.
2003: Patrizia Cioni; A Pesce; B Morozzo della Rocca; S Castelli; Mattia Falconi; L Parrilli; M Bolognesi; Giovanni B Strambini; Alessandro Desideri
Active-site copper and zinc ions modulate the quaternary structure of prokaryotic Cu,Zn superoxide dismutase.
Journal of molecular biology 2003;326(5):1351-60.
2002: Bruce A Kerwin; Byeong S Chang; Colin V Gegg; Margherita Gonnelli; Tiansheng Li; Giovanni B Strambini
Interactions between PEG and type I soluble tumor necrosis factor receptor: modulation by pH and by PEGylation at the N terminus.
Protein science : a publication of the Protein Society 2002;11(7):1825-33.
2002: Patrizia Cioni; Giovanni B Strambini
Effect of heavy water on protein flexibility.
Biophysical journal 2002;82(6):3246-53.
2002: Patrizia Cioni; Giovanni B Strambini
Tryptophan phosphorescence and pressure effects on protein structure.
Biochimica et biophysica acta 2002;1595(1-2):116-30.
2001: Patrizia Cioni; Maria Elena Stroppolo; Alessandro Desideri; Giovanni B Strambini
Dynamic features of the subunit interface of Cu,Zn superoxide dismutase as probed by tryptophan phosphorescence.
Archives of biochemistry and biophysics 2001;391(1):111-8.
2001: Mattia Falconi; Maria Elena Stroppolo; Patrizia Cioni; Giovanni B Strambini; Alessandro Sergi; M Ferrario; Alessandro Desideri
Dynamics-function correlation in Cu, Zn superoxide dismutase: a spectroscopic and molecular dynamics simulation study.
Biophysical journal 2001;80(6):2556-67.
2001: Edi Gabellieri; Giovanni B Strambini
Structural perturbations of azurin deposited on solid matrices as revealed by trp phosphorescence.
Biophysical journal 2001;80(5):2431-8.
2001: Margherita Gonnelli; Giovanni B Strambini
No effect of trimethylamine N-oxide on the internal dynamics of the protein native fold.
Biophysical chemistry 2001;89(1):77-85.
2000: Jaap Broos; Giovanni B Strambini; Margherita Gonnelli; Erwin P P Vos; M Koolhof; George T Robillard
Sensitive monitoring of the dynamics of a membrane-bound transport protein by tryptophan phosphorescence spectroscopy.
Biochemistry 2000;39(35):10877-83.
1999: Patrizia Cioni; Giovanni B Strambini
Pressure/temperature effects on protein flexibilty from acrylamide quenching of protein phosphorescence.
Journal of molecular biology 1999;291(4):955-64.
1998: Patrizia Cioni; L Erijman; Giovanni B Strambini
Phosphorescence emission of 7-azatryptophan and 5-hydroxytryptophan in fluid solutions and in alpha2 RNA polymerase.
Biochemical and biophysical research communications 1998;248(2):347-51.
1998: Giovanni B Strambini; Edi Gabellieri; Margherita Gonnelli; Sophie Rahuel-Clermont; Guy Branlant
Tyrosine quenching of tryptophan phosphorescence in glyceraldehyde-3-phosphate dehydrogenase from Bacillus stearothermophilus.
Biophysical journal 1998;74(6):3165-72.
1997: Margherita Gonnelli; Giovanni B Strambini
Time-resolved protein phosphorescence in the stopped-flow: denaturation of horse liver alcohol dehydrogenase by urea and guanidine hydrochloride.
Biochemistry 1997;36(51):16212-20.
1997: Patrizia Cioni; Giovanni B Strambini
Pressure-induced dissociation of yeast glyceraldehyde-3-phosphate dehydrogenase: heterogeneous kinetics and perturbations of subunit structure.
Biochemistry 1997;36(28):8586-93.
1997: Edi Gabellieri; Giovanni B Strambini; Alessandra Baracca; Giancarlo Solaini
Structural mapping of the epsilon-subunit of mitochondrial H(+)-ATPase complex (F1).
Biophysical journal 1997;72(4):1818-27.
1996: Patrizia Cioni; Giovanni B Strambini
Pressure effects on the structure of oligomeric proteins prior to subunit dissociation.
Journal of molecular biology 1996;263(5):789-99.
1996: Edi Gabellieri; Sophie Rahuel-Clermont; Guy Branlant; Giovanni B Strambini
Effects of NAD+ binding on the luminescence of tryptophans 84 and 310 of glyceraldehyde-3-phosphate dehydrogenase from Bacillus stearothermophilus.
Biochemistry 1996;35(38):12549-59.
1996: Giovanni B Strambini; Patrizia Cioni; P F Cook
Tryptophan luminescence as a probe of enzyme conformation along the O-acetylserine sulfhydrylase reaction pathway.
Biochemistry 1996;35(25):8392-400.
1996: Giovanni B Strambini; Edi Gabellieri
Proteins in frozen solutions: evidence of ice-induced partial unfolding.
Biophysical journal 1996;70(2):971-6.
1995: Margherita Gonnelli; Giovanni B Strambini
Phosphorescence lifetime of tryptophan in proteins.
Biochemistry 1995;34(42):13847-57.
1994: Patrizia Cioni; Giovanni B Strambini
Pressure effects on protein flexibility monomeric proteins.
Journal of molecular biology 1994;242(3):291-301.
1994: Edi Gabellieri; Giovanni B Strambini
Conformational changes in proteins induced by dynamic associations. A tryptophan phosphorescence study.
European journal of biochemistry / FEBS 1994;221(1):77-85.
1993: Margherita Gonnelli; Giovanni B Strambini
Glycerol effects on protein flexibility: a tryptophan phosphorescence study.
Biophysical journal 1993;65(1):131-7.
1993: Giancarlo Solaini; Alessandra Baracca; Giovanna Parenti Castelli; Giovanni B Strambini
Tryptophan phosphorescence as a structural probe of mitochondrial F1-ATPase epsilon-subunit.
European journal of biochemistry / FEBS 1993;214(3):729-34.
1993: Patrizia Cioni; A Puntoni; Giovanni B Strambini
Tryptophan phosphorescence as a monitor of the solution structure of phosphoglycerate kinase from yeast.
Biophysical chemistry 1993;46(1):47-55.
1992: Patrizia Cioni; J J Onuffer; Giovanni B Strambini
Characterization of tryptophan phosphorescence of aspartate aminotransferase from Escherichia coli.
European journal of biochemistry / FEBS 1992;209(2):759-64.
1992: Giovanni B Strambini; Patrizia Cioni; A Peracchi; Andrea Mozzarelli
Conformational changes and subunit communication in tryptophan synthase: effect of substrates and substrate analogs.
Biochemistry 1992;31(33):7535-42.
1992: Giovanni B Strambini; Patrizia Cioni; A Peracchi; Andrea Mozzarelli
Characterization of tryptophan and coenzyme luminescence in tryptophan synthase from Salmonella typhimurium.
Biochemistry 1992;31(33):7527-34.
1991: Giovanni B Strambini; S S Lehrer
Tryptophan phosphorescence of G-actin and F-actin.
European journal of biochemistry / FEBS 1991;195(3):645-51.
1990: Giovanni B Strambini; Edi Gabellieri
Temperature dependence of tryptophan phosphorescence in proteins.
Photochemistry and photobiology 1990;51(6):643-8.
1990: Giovanni B Strambini; Margherita Gonnelli; W C Galley
Room temperature phosphorescence of Trp-314 as a monitor of subunit communications in alcohol dehydrogenase from horse liver.
Biochemistry 1990;29(1):203-8.
1990: Giovanni B Strambini; Margherita Gonnelli
Tryptophan luminescence from liver alcohol dehydrogenase in its complexes with coenzyme. A comparative study of protein conformation in solution.
Biochemistry 1990;29(1):196-203.
1989: Patrizia Cioni; L Piras; Giovanni B Strambini
Tryptophan phosphorescence as a monitor of the structural role of metal ions in alkaline phosphatase.
European journal of biochemistry / FEBS 1989;185(3):573-9.
1989: Edi Gabellieri; Giovanni B Strambini
Phosphorescence properties of Trp-84 and Trp-310 in glyceraldehyde-3-phosphate dehydrogenase from Bacillus stearothermophilus.
Biophysical chemistry 1989;33(3):257-64.
1989: Patrizia Cioni; Giovanni B Strambini
Dynamical structure of glutamate dehydrogenase as monitored by tryptophan phosphorescence. Signal transmission following binding of allosteric effectors.
Journal of molecular biology 1989;207(1):237-47.
1989: Giovanni B Strambini; Patrizia Cioni; A Puntoni
Relationship between the conformation of glutamate dehydrogenase, the state of association of its subunit, and catalytic function.
Biochemistry 1989;28(9):3808-14.
1989: Giovanni B Strambini; Edi Gabellieri
Phosphorescence properties and protein structure surrounding tryptophan residues in yeast, pig, and rabbit glyceraldehyde-3-phosphate dehydrogenase.
Biochemistry 1989;28(1):160-6.
1988: Edi Gabellieri; Giovanni B Strambini; P Gualtieri
Tryptophan phosphorescence and the conformation of liver alcohol dehydrogenase in solution and in the crystalline state.
Biophysical chemistry 1988;30(1):61-7.
1987: Giovanni B Strambini; Edi Gabellieri
Phosphorescence anisotropy of liver alcohol dehydrogenase in the crystalline state. Apparent glasslike rigidity of the coenzyme-binding domain.
Biochemistry 1987;26(20):6527-30.
1987: Giovanni B Strambini; Patrizia Cioni; R A Felicioli
Characterization of tryptophan environments in glutamate dehydrogenases from temperature-dependent phosphorescence.
Biochemistry 1987;26(16):4968-75.
1986: Margherita Gonnelli; Giovanni B Strambini
The rate of equine liver alcohol dehydrogenase denaturation by urea. Dependence on temperature and denaturant concentration.
Biophysical chemistry 1986;24(2):161-7.
1986: Giovanni B Strambini; Margherita Gonnelli
Effects of urea and guanidine hydrochloride on the activity and dynamical structure of equine liver alcohol dehydrogenase.
Biochemistry 1986;25(9):2471-6.
1984: Giovanni B Strambini; Edi Gabellieri
Intrinsic phosphorescence from proteins in the solid state.
Photochemistry and photobiology 1984;39(6):725-9.