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JoAnne Stubbe

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Joseph A Cotruvo; Troy A Stich; R David Britt; JoAnne Stubbe
Mechanism of assembly of the dimanganese-tyrosyl radical cofactor of class Ib ribonucleotide reductase: enzymatic generation of superoxide is required for tyrosine oxidation via a Mn(III)Mn(IV) intermediate.
Amie K Boal; Joseph A Cotruvo; JoAnne Stubbe; Amy C Rosenzweig
The dimanganese(II) site of Bacillus subtilis class Ib ribonucleotide reductase.
Ellen C Minnihan; Mohammad R Seyedsayamdost; Ulla Uhlin; JoAnne Stubbe
Kinetics of radical intermediate formation and deoxynucleotide production in 3-aminotyrosine-substituted Escherichia coli ribonucleotide reductases.

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All Publications

2013: Joseph A Cotruvo; Troy A Stich; R David Britt; JoAnne Stubbe
Mechanism of assembly of the dimanganese-tyrosyl radical cofactor of class Ib ribonucleotide reductase: enzymatic generation of superoxide is required for tyrosine oxidation via a Mn(III)Mn(IV) intermediate.
Journal of the American Chemical Society 2013;135(10):4027-39.
2012: Amie K Boal; Joseph A Cotruvo; JoAnne Stubbe; Amy C Rosenzweig
The dimanganese(II) site of Bacillus subtilis class Ib ribonucleotide reductase.
Biochemistry 2012;51(18):3861-71.
2011: Ellen C Minnihan; Mohammad R Seyedsayamdost; Ulla Uhlin; JoAnne Stubbe
Kinetics of radical intermediate formation and deoxynucleotide production in 3-aminotyrosine-substituted Escherichia coli ribonucleotide reductases.
Journal of the American Chemical Society 2011;133(24):9430-40.
2011: Yimon Aye; JoAnne Stubbe
Clofarabine 5'-di and -triphosphates inhibit human ribonucleotide reductase by altering the quaternary structure of its large subunit.
Proceedings of the National Academy of Sciences of the United States of America 2011;108(24):9815-20.
2011: Joseph A Cotruvo; JoAnne Stubbe
Class I ribonucleotide reductases: metallocofactor assembly and repair in vitro and in vivo.
Annual review of biochemistry 2011;80():733-67.
2011: JoAnne Stubbe
Biochemistry. The two faces of SAM.
Science (New York, N.Y.) 2011;332(6029):544-5.
2011: JoAnne Stubbe; Joseph A Cotruvo
Control of metallation and active cofactor assembly in the class Ia and Ib ribonucleotide reductases: diiron or dimanganese?
Current opinion in chemical biology 2011;15(2):284-90.
2010: Amie K Boal; Joseph A Cotruvo; JoAnne Stubbe; Amy C Rosenzweig
Structural basis for activation of class Ib ribonucleotide reductase.
Science (New York, N.Y.) 2010;329(5998):1526-30.
2010: Joseph A Cotruvo; JoAnne Stubbe
An active dimanganese(III)-tyrosyl radical cofactor in Escherichia coli class Ib ribonucleotide reductase.
Biochemistry 2010;49(6):1297-309.
2009: Ellen C Minnihan; Mohammad R Seyedsayamdost; JoAnne Stubbe
Use of 3-aminotyrosine to examine the pathway dependence of radical propagation in Escherichia coli ribonucleotide reductase.
Biochemistry 2009;48(51):12125-32.
2009: Ping Li; Sumit Chakraborty; JoAnne Stubbe
Detection of covalent and noncovalent intermediates in the polymerization reaction catalyzed by a C149S class III polyhydroxybutyrate synthase.
Biochemistry 2009;48(39):9202-11.
2009: Mohammad R Seyedsayamdost; JoAnne Stubbe
Replacement of Y730 and Y731 in the alpha2 subunit of Escherichia coli ribonucleotide reductase with 3-aminotyrosine using an evolved suppressor tRNA/tRNA-synthetase pair.
Methods in enzymology 2009;462():45-76.
2008: Michael A Marletta; JoAnne Stubbe
Catalysis and regulation. Editorial overview.
Current opinion in structural biology 2008;18(6):641-3.
2008: A Quamrul Hassan; JoAnne Stubbe
Mapping the subunit interface of ribonucleotide reductase (RNR) using photo cross-linking.
Bioorganic & medicinal chemistry letters 2008;18(22):5923-5.
2008: Yang David Lee; Jun Wang; JoAnne Stubbe; Stephen J Elledge
Dif1 is a DNA-damage-regulated facilitator of nuclear import for ribonucleotide reductase.
Molecular cell 2008;32(1):70-80.
2008: Joseph A Cotruvo; JoAnne Stubbe
NrdI, a flavodoxin involved in maintenance of the diferric-tyrosyl radical cofactor in Escherichia coli class Ib ribonucleotide reductase.
Proceedings of the National Academy of Sciences of the United States of America 2008;105(38):14383-8.
2008: Mariya Morar; Aaron A Hoskins; JoAnne Stubbe; Steven E Ealick
Formylglycinamide ribonucleotide amidotransferase from Thermotoga maritima: structural insights into complex formation.
Biochemistry 2008;47(30):7816-30.
2008: Jingyang Chen; Manas K Ghorai; Grace Kenney; JoAnne Stubbe
Mechanistic studies on bleomycin-mediated DNA damage: multiple binding modes can result in double-stranded DNA cleavage.
Nucleic acids research 2008;36(11):3781-90.
2008: Daniela Hristova; Chia-Hung Wu; Wei Jiang; Carsten Krebs; JoAnne Stubbe
Importance of the maintenance pathway in the regulation of the activity of Escherichia coli ribonucleotide reductase.
Biochemistry 2008;47(13):3989-99.
2008: Jingyang Chen; François-Yves Dupradeau; David A Case; Christopher J Turner; JoAnne Stubbe
DNA oligonucleotides with A, T, G or C opposite an abasic site: structure and dynamics.
Nucleic acids research 2008;36(1):253-62.
2007: Mohammad R Seyedsayamdost; Clement T Y Chan; Veronica Mugnaini; JoAnne Stubbe; Marina Bennati
PELDOR spectroscopy with DOPA-beta2 and NH2Y-alpha2s: distance measurements between residues involved in the radical propagation pathway of E. coli ribonucleotide reductase.
Journal of the American Chemical Society 2007;129(51):15748-9.
2007: Mohammad R Seyedsayamdost; Jianming Xie; Clement T Y Chan; Peter G Schultz; JoAnne Stubbe
Site-specific insertion of 3-aminotyrosine into subunit alpha2 of E. coli ribonucleotide reductase: direct evidence for involvement of Y730 and Y731 in radical propagation.
Journal of the American Chemical Society 2007;129(48):15060-71.
2007: Steven Y Reece; Mohammad R Seyedsayamdost; JoAnne Stubbe; Daniel G Nocera
Direct observation of a transient tyrosine radical competent for initiating turnover in a photochemical ribonucleotide reductase.
Journal of the American Chemical Society 2007;129(45):13828-30.
2007: Bingzi Chen; Xinfeng Zhou; Koli Taghizadeh; Jingyang Chen; JoAnne Stubbe; Peter C Dedon
GC/MS methods to quantify the 2-deoxypentos-4-ulose and 3'-phosphoglycolate pathways of 4' oxidation of 2-deoxyribose in DNA: application to DNA damage produced by gamma radiation and bleomycin.
Chemical research in toxicology 2007;20(11):1701-8.
2007: Chia-Hung Wu; Wei Jiang; Carsten Krebs; JoAnne Stubbe
YfaE, a ferredoxin involved in diferric-tyrosyl radical maintenance in Escherichia coli ribonucleotide reductase.
Biochemistry 2007;46(41):11577-88.
2007: Jun Wang; Gregory J S Lohman; JoAnne Stubbe
Enhanced subunit interactions with gemcitabine-5'-diphosphate inhibit ribonucleotide reductases.
Proceedings of the National Academy of Sciences of the United States of America 2007;104(36):14324-9.
2007: Steven Y Reece; Mohammad R Seyedsayamdost; JoAnne Stubbe; Daniel G Nocera
Photoactive peptides for light-initiated tyrosyl radical generation and transport into ribonucleotide reductase.
Journal of the American Chemical Society 2007;129(27):8500-9.
2007: Jingyang Chen; François-Yves Dupradeau; David A Case; Christopher J Turner; JoAnne Stubbe
Nuclear magnetic resonance structural studies and molecular modeling of duplex DNA containing normal and 4'-oxidized abasic sites.
Biochemistry 2007;46(11):3096-107.
2007: Aaron A Hoskins; Mariya Morar; T Joseph Kappock; Irimpan I Mathews; Judith B Zaugg; Timothy E Barder; Paul Peng; Akimitsu Okamoto; Steven E Ealick; JoAnne Stubbe
N5-CAIR mutase: role of a CO2 binding site and substrate movement in catalysis.
Biochemistry 2007;46(10):2842-55.
2007: Mohammad R Seyedsayamdost; JoAnne Stubbe
Forward and reverse electron transfer with the Y356DOPA-beta2 heterodimer of E. coli ribonucleotide reductase.
Journal of the American Chemical Society 2007;129(8):2226-7.
2007: Mohammad R Seyedsayamdost; Cyril S Yee; JoAnne Stubbe
Site-specific incorporation of fluorotyrosines into the R2 subunit of E. coli ribonucleotide reductase by expressed protein ligation.
Nature protocols 2007;2(5):1225-35.
2006: Mariya Morar; Ruchi Anand; Aaron A Hoskins; JoAnne Stubbe; Steven E Ealick
Complexed structures of formylglycinamide ribonucleotide amidotransferase from Thermotoga maritima describe a novel ATP binding protein superfamily.
Biochemistry 2006;45(50):14880-95.
2006: Steven Y Reece; Mohammad R Seyedsayamdost; JoAnne Stubbe; Daniel G Nocera
Electron transfer reactions of fluorotyrosyl radicals.
Journal of the American Chemical Society 2006;128(42):13654-5.
2006: Allison D Ortigosa; Daniela Hristova; Deborah L Perlstein; Zhen Zhang; Mingxia Huang; JoAnne Stubbe
Determination of the in vivo stoichiometry of tyrosyl radical per betabeta' in Saccharomyces cerevisiae ribonucleotide reductase.
Biochemistry 2006;45(40):12282-94.
2006: V P Denysenkov; Thomas F Prisner; JoAnne Stubbe; Marina Bennati
High-field pulsed electron-electron double resonance spectroscopy to determine the orientation of the tyrosyl radicals in ribonucleotide reductase.
Proceedings of the National Academy of Sciences of the United States of America 2006;103(36):13386-90.
2006: Steven Y Reece; Justin M Hodgkiss; JoAnne Stubbe; Daniel G Nocera
Proton-coupled electron transfer: the mechanistic underpinning for radical transport and catalysis in biology.
Philosophical transactions of the Royal Society of London. Series B, Biological sciences 2006;361(1472):1351-64.
2006: Mohammad R Seyedsayamdost; JoAnne Stubbe
Site-specific replacement of Y356 with 3,4-dihydroxyphenylalanine in the beta2 subunit of E. coli ribonucleotide reductase.
Journal of the American Chemical Society 2006;128(8):2522-3.
2006: Mohammad R Seyedsayamdost; Steven Y Reece; Daniel G Nocera; JoAnne Stubbe
Mono-, di-, tri-, and tetra-substituted fluorotyrosines: new probes for enzymes that use tyrosyl radicals in catalysis.
Journal of the American Chemical Society 2006;128(5):1569-79.
2006: Mohammad R Seyedsayamdost; Cyril S Yee; Steven Y Reece; Daniel G Nocera; JoAnne Stubbe
pH Rate profiles of FnY356-R2s (n = 2, 3, 4) in Escherichia coli ribonucleotide reductase: evidence that Y356 is a redox-active amino acid along the radical propagation pathway.
Journal of the American Chemical Society 2006;128(5):1562-8.
2006: Zhen Zhang; Xiuxiang An; Kui Yang; Deborah L Perlstein; Leslie Hicks; Neil L Kelleher; JoAnne Stubbe; Mingxia Huang
Nuclear localization of the Saccharomyces cerevisiae ribonucleotide reductase small subunit requires a karyopherin and a WD40 repeat protein.
Proceedings of the National Academy of Sciences of the United States of America 2006;103(5):1422-7.
2005: Deborah L Perlstein; Jie Ge; Allison D Ortigosa; John H Robblee; Zhen Zhang; Mingxia Huang; JoAnne Stubbe
The active form of the Saccharomyces cerevisiae ribonucleotide reductase small subunit is a heterodimer in vitro and in vivo.
Biochemistry 2005;44(46):15366-77.
2005: Marina Bennati; John H Robblee; Veronica Mugnaini; JoAnne Stubbe; Jack H Freed; Peter P Borbat
EPR distance measurements support a model for long-range radical initiation in E. coli ribonucleotide reductase.
Journal of the American Chemical Society 2005;127(43):15014-5.
2005: Adam G Lawrence; Joerg Schoenheit; Aimin He; Jiamin Tian; Pinghua Liu; JoAnne Stubbe; Anthony J Sinskey
Transcriptional analysis of Ralstonia eutropha genes related to poly-(R)-3-hydroxybutyrate homeostasis during batch fermentation.
Applied microbiology and biotechnology 2005;68(5):663-72.
2005: Adam G Lawrence; Joonho Choi; ChoKyun Rha; JoAnne Stubbe; Anthony J Sinskey
In vitro analysis of the chain termination reaction in the synthesis of poly-(R)-beta-hydroxybutyrate by the class III synthase from Allochromatium vinosum.
Biomacromolecules 2005;6(4):2113-9.
2005: Jiamin Tian; Anthony J Sinskey; JoAnne Stubbe
Class III polyhydroxybutyrate synthase: involvement in chain termination and reinitiation.
Biochemistry 2005;44(23):8369-77.
2005: Jörg Fritscher; Erin Artin; Stanislaw Wnuk; Galit Bar; John H Robblee; Sylwia Kacprzak; Martin Kaupp; Robert G Griffin; Marina Bennati; JoAnne Stubbe
Structure of the nitrogen-centered radical formed during inactivation of E. coli ribonucleotide reductase by 2'-azido-2'-deoxyuridine-5'-diphosphate: trapping of the 3'-ketonucleotide.
Journal of the American Chemical Society 2005;127(21):7729-38.
2005: Jiamin Tian; Aimin He; Adam G Lawrence; Pinghua Liu; Nicki Watson; Anthony J Sinskey; JoAnne Stubbe
Analysis of transient polyhydroxybutyrate production in Wautersia eutropha H16 by quantitative Western analysis and transmission electron microscopy.
Journal of bacteriology 2005;187(11):3825-32.
2005: Jiamin Tian; Anthony J Sinskey; JoAnne Stubbe
Kinetic studies of polyhydroxybutyrate granule formation in Wautersia eutropha H16 by transmission electron microscopy.
Journal of bacteriology 2005;187(11):3814-24.
2005: Steven Y Reece; JoAnne Stubbe; Daniel G Nocera
pH Dependence of charge transfer between tryptophan and tyrosine in dipeptides.
Biochimica et biophysica acta 2005;1706(3):232-8.
2005: Jiamin Tian; Anthony J Sinskey; JoAnne Stubbe
Detection of intermediates from the polymerization reaction catalyzed by a D302A mutant of class III polyhydroxyalkanoate (PHA) synthase.
Biochemistry 2005;44(5):1495-503.
2005: Jingyang Chen; JoAnne Stubbe
Bleomycins: towards better therapeutics.
Nature reviews. Cancer 2005;5(2):102-12.
2005: Roman Davydov; Behnaz Behrouzian; Stoyan K Smoukov; JoAnne Stubbe; Brian M Hoffman; John Shanklin
Effect of substrate on the diiron(III) site in stearoyl acyl carrier protein delta 9-desaturase as disclosed by cryoreduction electron paramagnetic resonance/electron nuclear double resonance spectroscopy.
Biochemistry 2005;44(4):1309-15.
2005: JoAnne Stubbe; Jiamin Tian; Aimin He; Anthony J Sinskey; Adam G Lawrence; Pinghua Liu
Nontemplate-dependent polymerization processes: polyhydroxyalkanoate synthases as a paradigm.
Annual review of biochemistry 2005;74():433-80.
2004: Michelle C Y Chang; Cyril S Yee; Daniel G Nocera; JoAnne Stubbe
Site-specific replacement of a conserved tyrosine in ribonucleotide reductase with an aniline amino acid: a mechanistic probe for a redox-active tyrosine.
Journal of the American Chemical Society 2004;126(51):16702-3.
2004: Ruchi Anand; Aaron A Hoskins; Eric M Bennett; Michael D Sintchak; JoAnne Stubbe; Steven E Ealick
A model for the Bacillus subtilis formylglycinamide ribonucleotide amidotransferase multiprotein complex.
Biochemistry 2004;43(32):10343-52.
2004: Ruchi Anand; Aaron A Hoskins; JoAnne Stubbe; Steven E Ealick
Domain organization of Salmonella typhimurium formylglycinamide ribonucleotide amidotransferase revealed by X-ray crystallography.
Biochemistry 2004;43(32):10328-42.
2004: Aaron A Hoskins; Ruchi Anand; Steven E Ealick; JoAnne Stubbe
The formylglycinamide ribonucleotide amidotransferase complex from Bacillus subtilis: metabolite-mediated complex formation.
Biochemistry 2004;43(32):10314-27.
2004: Monika Sommerhalter; Walter C Voegtli; Deborah L Perlstein; Jie Ge; JoAnne Stubbe; Amy C Rosenzweig
Structures of the yeast ribonucleotide reductase Rnr2 and Rnr4 homodimers.
Biochemistry 2004;43(24):7736-42.
2004: Jingyang Chen; JoAnne Stubbe
Synthesis and characterization of oligonucleotides containing a 4'-keto abasic site.
Biochemistry 2004;43(18):5278-86.
2004: Michelle C Y Chang; Cyril S Yee; JoAnne Stubbe; Daniel G Nocera
Turning on ribonucleotide reductase by light-initiated amino acid radical generation.
Proceedings of the National Academy of Sciences of the United States of America 2004;101(18):6882-7.
2004: Jingyang Chen; JoAnne Stubbe
Bleomycins: new methods will allow reinvestigation of old issues.
Current opinion in chemical biology 2004;8(2):175-81.
2003: Cyril S Yee; Mohammad R Seyedsayamdost; Michelle C Y Chang; Daniel G Nocera; JoAnne Stubbe
Generation of the R2 subunit of ribonucleotide reductase by intein chemistry: insertion of 3-nitrotyrosine at residue 356 as a probe of the radical initiation process.
Biochemistry 2003;42(49):14541-52.
2003: Marina Bennati; Axel Weber; Jelena Antonic; Deborah L Perlstein; John H Robblee; JoAnne Stubbe
Pulsed ELDOR spectroscopy measures the distance between the two tyrosyl dadicals in the R2 subunit of the E. coli ribonucleotide reductase.
Journal of the American Chemical Society 2003;125(49):14988-9.
2003: JoAnne Stubbe
Radicals with a controlled lifestyle.
Chemical communications (Cambridge, England) 2003;(20):2511-3.
2003: JoAnne Stubbe; Jiamin Tian
Polyhydroxyalkanoate (PHA) hemeostasis: the role of PHA synthase.
Natural product reports 2003;20(5):445-57.
2003: Cyril S Yee; Michelle C Y Chang; Jie Ge; Daniel G Nocera; JoAnne Stubbe
2,3-difluorotyrosine at position 356 of ribonucleotide reductase R2: a probe of long-range proton-coupled electron transfer.
Journal of the American Chemical Society 2003;125(35):10506-7.
2003: Jie Ge; Guixue Yu; Mark A Ator; JoAnne Stubbe
Pre-steady-state and steady-state kinetic analysis of E. coli class I ribonucleotide reductase.
Biochemistry 2003;42(34):10071-83.
2003: Gregory M York; Joachim Lupberger; Jiamin Tian; Adam G Lawrence; JoAnne Stubbe; Anthony J Sinskey
Ralstonia eutropha H16 encodes two and possibly three intracellular Poly[D-(-)-3-hydroxybutyrate] depolymerase genes.
Journal of bacteriology 2003;185(13):3788-94.
2003: JoAnne Stubbe; Daniel G Nocera; Cyril S Yee; Michelle C Y Chang
Radical initiation in the class I ribonucleotide reductase: long-range proton-coupled electron transfer?
Chemical reviews 2003;103(6):2167-201.
2003: Ruojin Yao; Zhen Zhang; Xiuxiang An; Brigid Bucci; Deborah L Perlstein; JoAnne Stubbe; Mingxia Huang
Subcellular localization of yeast ribonucleotide reductase regulated by the DNA replication and damage checkpoint pathways.
Proceedings of the National Academy of Sciences of the United States of America 2003;100(11):6628-33.
2003: JoAnne Stubbe
Di-iron-tyrosyl radical ribonucleotide reductases.
Current opinion in chemical biology 2003;7(2):183-8.
2002: Hans-Dieter Junker; Silvia T Hoehn; Richard C Bunt; Vasilios Marathius; Jingyang Chen; Christopher J Turner; JoAnne Stubbe
Synthesis, characterization and solution structure of tethered oligonucleotides containing an internal 3'-phosphoglycolate, 5'-phosphate gapped lesion.
Nucleic acids research 2002;30(24):5497-508.
2002: Wei Wu; Dana E Vanderwall; Christopher J Turner; Silvia T Hoehn; Jingyang Chen; John W Kozarich; JoAnne Stubbe
Solution structure of the hydroperoxide of Co(III) phleomycin complexed with d(CCAGGCCTGG)2: evidence for binding by partial intercalation.
Nucleic acids research 2002;30(22):4881-91.
2002: Michelle C Y Chang; Scott E Miller; Scott D Carpenter; JoAnne Stubbe; Daniel G Nocera
Nanosecond generation of tyrosyl radicals via laser-initiated decaging of oxalate-modified amino acids.
The Journal of organic chemistry 2002;67(19):6820-2.
2002: Michael D Sintchak; Gitrada Arjara; Brenda A Kellogg; JoAnne Stubbe; Catherine L Drennan
The crystal structure of class II ribonucleotide reductase reveals how an allosterically regulated monomer mimics a dimer.
Nature structural biology 2002;9(4):293-300.
2002: Gregory M York; JoAnne Stubbe; Anthony J Sinskey
The Ralstonia eutropha PhaR protein couples synthesis of the PhaP phasin to the presence of polyhydroxybutyrate in cells and promotes polyhydroxybutyrate production.
Journal of bacteriology 2002;184(1):59-66.
2001: W Yuan; Y Jia; Jiamin Tian; K D Snell; U Müh; Anthony J Sinskey; R H Lambalot; Christopher T Walsh; JoAnne Stubbe
Class I and III polyhydroxyalkanoate synthases from Ralstonia eutropha and Allochromatium vinosum: characterization and substrate specificity studies.
Archives of biochemistry and biophysics 2001;394(1):87-98.
2001: Walter C Voegtli; Jie Ge; Deborah L Perlstein; JoAnne Stubbe; Amy C Rosenzweig
Structure of the yeast ribonucleotide reductase Y2Y4 heterodimer.
Proceedings of the National Academy of Sciences of the United States of America 2001;98(18):10073-8.
2001: Jie Ge; Deborah L Perlstein; HH Nguyen; Galit Bar; Robert G Griffin; JoAnne Stubbe
Why multiple small subunits (Y2 and Y4) for yeast ribonucleotide reductase? Toward understanding the role of Y4.
Proceedings of the National Academy of Sciences of the United States of America 2001;98(18):10067-72.
2001: Silvia T Hoehn; Christopher J Turner; JoAnne Stubbe
Solution structure of an oligonucleotide containing an abasic site: evidence for an unusual deoxyribose conformation.
Nucleic acids research 2001;29(16):3413-23.
2001: Gregory M York; B H Junker; JoAnne Stubbe; Anthony J Sinskey
Accumulation of the PhaP phasin of Ralstonia eutropha is dependent on production of polyhydroxybutyrate in cells.
Journal of bacteriology 2001;183(14):4217-26.
2001: Silvia T Hoehn; H D Junker; Richard C Bunt; Christopher J Turner; JoAnne Stubbe
Solution structure of Co(III)-bleomycin-OOH bound to a phosphoglycolate lesion containing oligonucleotide: implications for bleomycin-induced double-strand DNA cleavage.
Biochemistry 2001;40(20):5894-905.
2001: Galit Bar; Marina Bennati; HH Nguyen; Jie Ge; JoAnne Stubbe; Robert G Griffin
High-frequency (140-GHz) time domain EPR and ENDOR spectroscopy: the tyrosyl radical-diiron cofactor in ribonucleotide reductase from yeast.
Journal of the American Chemical Society 2001;123(15):3569-76.
2001: Gregory M York; JoAnne Stubbe; Anthony J Sinskey
New insight into the role of the PhaP phasin of Ralstonia eutropha in promoting synthesis of polyhydroxybutyrate.
Journal of bacteriology 2001;183(7):2394-7.
2001: JoAnne Stubbe; Jie Ge; Cyril S Yee
The evolution of ribonucleotide reduction revisited.
Trends in biochemical sciences 2001;26(2):93-9.
2001: Y Jia; W Yuan; J Wodzinska; C Park; Anthony J Sinskey; JoAnne Stubbe
Mechanistic studies on class I polyhydroxybutyrate (PHB) synthase from Ralstonia eutropha: class I and III synthases share a similar catalytic mechanism.
Biochemistry 2001;40(4):1011-9.
2000: JoAnne Stubbe
Ribonucleotide reductases: the link between an RNA and a DNA world?
Current opinion in structural biology 2000;10(6):731-6.
2000: T Joseph Kappock; Steven E Ealick; JoAnne Stubbe
Modular evolution of the purine biosynthetic pathway.
Current opinion in chemical biology 2000;4(5):567-72.
2000: A Harsch; L A Marzilli; Richard C Bunt; JoAnne Stubbe; Paul Vouros
Accurate and rapid modeling of iron-bleomycin-induced DNA damage using tethered duplex oligonucleotides and electrospray ionization ion trap mass spectrometric analysis.
Nucleic acids research 2000;28(9):1978-85.
2000: Y Jia; T Joseph Kappock; T Frick; Anthony J Sinskey; JoAnne Stubbe
Lipases provide a new mechanistic model for polyhydroxybutyrate (PHB) synthases: characterization of the functional residues in Chromatium vinosum PHB synthase.
Biochemistry 2000;39(14):3927-36.
1999: Irimpan I Mathews; T Joseph Kappock; JoAnne Stubbe; Steven E Ealick
Crystal structure of Escherichia coli PurE, an unusual mutase in the purine biosynthetic pathway.
Structure (London, England : 1993) 1999;7(11):1395-406.
1999: HH Nguyen; Jie Ge; Deborah L Perlstein; JoAnne Stubbe
Purification of ribonucleotide reductase subunits Y1, Y2, Y3, and Y4 from yeast: Y4 plays a key role in diiron cluster assembly.
Proceedings of the National Academy of Sciences of the United States of America 1999;96(22):12339-44.
1999: E J Mueller; S Oh; E Kavalerchik; T Joseph Kappock; E Meyer; C Li; Steven E Ealick; JoAnne Stubbe
Investigation of the ATP binding site of Escherichia coli aminoimidazole ribonucleotide synthetase using affinity labeling and site-directed mutagenesis.
Biochemistry 1999;38(31):9831-9.
1999: CC Lawrence; Marina Bennati; H V Obias; Galit Bar; Robert G Griffin; JoAnne Stubbe
High-field EPR detection of a disulfide radical anion in the reduction of cytidine 5'-diphosphate by the E441Q R1 mutant of Escherichia coli ribonucleotide reductase.
Proceedings of the National Academy of Sciences of the United States of America 1999;96(16):8979-84.
1999: Marina Bennati; CT Farrar; Jeffrey A Bryant; S J Inati; Volker Weis; Gary J Gerfen; Pamela J Riggs-Gelasco; JoAnne Stubbe; Robert G Griffin
Pulsed electron-nuclear double resonance (ENDOR) at 140 GHz.
Journal of magnetic resonance (San Diego, Calif. : 1997) 1999;138(2):232-43.
1999: CC Lawrence; Gary J Gerfen; V Samano; R Nitsche; Morris J Robins; János Rétey; JoAnne Stubbe
Binding of Cob(II)alamin to the adenosylcobalamin-dependent ribonucleotide reductase from Lactobacillus leichmannii. Identification of dimethylbenzimidazole as the axial ligand.
The Journal of biological chemistry 1999;274(11):7039-42.
1999: E Meyer; T Joseph Kappock; C Osuji; JoAnne Stubbe
Evidence for the direct transfer of the carboxylate of N5-carboxyaminoimidazole ribonucleotide (N5-CAIR) to generate 4-carboxy-5-aminoimidazole ribonucleotide catalyzed by Escherichia coli PurE, an N5-CAIR mutase.
Biochemistry 1999;38(10):3012-8.
1999: Stuart Licht; CC Lawrence; JoAnne Stubbe
Thermodynamic and kinetic studies on carbon-cobalt bond homolysis by ribonucleoside triphosphate reductase: the importance of entropy in catalysis.
Biochemistry 1999;38(4):1234-42.
1999: Stuart Licht; S Booker; JoAnne Stubbe
Studies on the catalysis of carbon-cobalt bond homolysis by ribonucleoside triphosphate reductase: evidence for concerted carbon-cobalt bond homolysis and thiyl radical formation.
Biochemistry 1999;38(4):1221-33.
1999: U Müh; Anthony J Sinskey; D P Kirby; William Stratton Lane; JoAnne Stubbe
PHA synthase from chromatium vinosum: cysteine 149 is involved in covalent catalysis.
Biochemistry 1999;38(2):826-37.
1998: W Wang; T Joseph Kappock; JoAnne Stubbe; Steven E Ealick
X-ray crystal structure of glycinamide ribonucleotide synthetase from Escherichia coli.
Biochemistry 1998;37(45):15647-62.
1998: JoAnne Stubbe; Pamela J Riggs-Gelasco
Harnessing free radicals: formation and function of the tyrosyl radical in ribonucleotide reductase.
Trends in biochemical sciences 1998;23(11):438-43.
1998: CC Lawrence; JoAnne Stubbe
The function of adenosylcobalamin in the mechanism of ribonucleoside triphosphate reductase from Lactobacillus leichmannii.
Current opinion in chemical biology 1998;2(5):650-5.
1998: Wilfred Adrianus van der Donk; G Yu; L Pérez; R J Sanchez; JoAnne Stubbe; V Samano; Morris J Robins
Detection of a new substrate-derived radical during inactivation of ribonucleotide reductase from Escherichia coli by gemcitabine 5'-diphosphate.
Biochemistry 1998;37(18):6419-26.
1998: W Tong; D Burdi; Pamela J Riggs-Gelasco; S Chen; Dale E Edmondson; Boi Hanh Huynh; JoAnne Stubbe; S Han; Andrew S Arvai; J Tainer
Characterization of Y122F R2 of Escherichia coli ribonucleotide reductase by time-resolved physical biochemical methods and X-ray crystallography.
Biochemistry 1998;37(17):5840-8.
1998: D J Silva; JoAnne Stubbe; V Samano; Morris J Robins
Gemcitabine 5'-triphosphate is a stoichiometric mechanism-based inhibitor of Lactobacillus leichmannii ribonucleoside triphosphate reductase: evidence for thiyl radical-mediated nucleotide radical formation.
Biochemistry 1998;37(16):5528-35.
1998: JoAnne Stubbe
Ribonucleotide reductases in the twenty-first century.
Proceedings of the National Academy of Sciences of the United States of America 1998;95(6):2723-4.
1997: Dana E Vanderwall; S M Lui; W Wu; Christopher J Turner; John W Kozarich; JoAnne Stubbe
A model of the structure of HOO-Co.bleomycin bound to d(CCAGTACTGG): recognition at the d(GpT) site and implications for double-stranded DNA cleavage.
Chemistry & biology 1997;4(5):373-87.
1997: M Bansal; JoAnne Stubbe; John W Kozarich
Effects of hypoxanthine substitution on bleomycin-mediated DNA strand degradation in DNA-RNA hybrids.
Nucleic acids research 1997;25(9):1846-53.
1997: M Bansal; J S Lee; JoAnne Stubbe; John W Kozarich
Mechanistic analyses of site-specific degradation in DNA-RNA hybrids by prototypic DNA cleavers.
Nucleic acids research 1997;25(9):1836-45.
1997: S J Sim; K D Snell; S A Hogan; JoAnne Stubbe; ChoKyun Rha; Anthony J Sinskey
PHA synthase activity controls the molecular weight and polydispersity of polyhydroxybutyrate in vivo.
Nature biotechnology 1997;15(1):63-7.
1996: JoAnne Stubbe
One of nature's macromolecular machines demystified.
Science (New York, N.Y.) 1996;274(5290):1152-3.
1996: Wilfred Adrianus van der Donk; C Zeng; Klaus Biemann; JoAnne Stubbe; A Hanlon; Jack E Kyte
Identification of an active site residue of the R1 subunit of ribonucleotide reductase from Escherichia coli: characterization of substrate-induced polypeptide cleavage by C225SR1.
Biochemistry 1996;35(31):10058-67.
1996: Wilfred Adrianus van der Donk; G Yu; D J Silva; JoAnne Stubbe; J R McCarthy; E T Jarvi; D P Matthews; R J Resvick; E Wagner
Inactivation of ribonucleotide reductase by (E)-2'-fluoromethylene-2'-deoxycytidine 5'-diphosphate: a paradigm for nucleotide mechanism-based inhibitors.
Biochemistry 1996;35(25):8381-91.
1996: Stuart Licht; Gary J Gerfen; JoAnne Stubbe
Thiyl radicals in ribonucleotide reductases.
Science (New York, N.Y.) 1996;271(5248):477-81.
1995: JoAnne Stubbe; Wilfred Adrianus van der Donk
Ribonucleotide reductases: radical enzymes with suicidal tendencies.
Chemistry & biology 1995;2(12):793-801.
1995: J Rudolph; JoAnne Stubbe
Investigation of the mechanism of phosphoribosylamine transfer from glutamine phosphoribosylpyrophosphate amidotransferase to glycinamide ribonucleotide synthetase.
Biochemistry 1995;34(7):2241-50.
1995: M J Absalon; W Wu; John W Kozarich; JoAnne Stubbe
Sequence-specific double-strand cleavage of DNA by Fe-bleomycin. 2. Mechanism and dynamics.
Biochemistry 1995;34(6):2076-86.
1995: M J Absalon; John W Kozarich; JoAnne Stubbe
Sequence-specific double-strand cleavage of DNA by Fe-bleomycin. 1. The detection of sequence-specific double-strand breaks using hairpin oligonucleotides.
Biochemistry 1995;34(6):2065-75.
1995: J Martin Bollinger; W H Tong; N Ravi; Boi Hanh Huynh; Dale E Edmondson; JoAnne Stubbe
Use of rapid kinetics methods to study the assembly of the diferric-tyrosyl radical cofactor of E. coli ribonucleotide reductase.
Methods in enzymology 1995;258():278-303.
1994: JoAnne Stubbe
Binding site revealed of nature's most beautiful cofactor.
Science (New York, N.Y.) 1994;266(5191):1663-4.
1994: S Booker; Stuart Licht; J Broderick; JoAnne Stubbe
Coenzyme B12-dependent ribonucleotide reductase: evidence for the participation of five cysteine residues in ribonucleotide reduction.
Biochemistry 1994;33(42):12676-85.
1994: Steven M Firestine; S W Poon; E J Mueller; JoAnne Stubbe; V Jo Davisson
Reactions catalyzed by 5-aminoimidazole ribonucleotide carboxylases from Escherichia coli and Gallus gallus: a case for divergent catalytic mechanisms.
Biochemistry 1994;33(39):11927-34.
1994: T U Gerngross; K D Snell; O P Peoples; Anthony J Sinskey; E Csuhai; S Masamune; JoAnne Stubbe
Overexpression and purification of the soluble polyhydroxyalkanoate synthase from Alcaligenes eutrophus: evidence for a required posttranslational modification for catalytic activity.
Biochemistry 1994;33(31):9311-20.
1994: E J Mueller; E Meyer; J Rudolph; V Jo Davisson; JoAnne Stubbe
N5-carboxyaminoimidazole ribonucleotide: evidence for a new intermediate and two new enzymatic activities in the de novo purine biosynthetic pathway of Escherichia coli.
Biochemistry 1994;33(8):2269-78.
1993: L D Thornburg; JoAnne Stubbe
Mechanism-based inactivation of thymine hydroxylase, an alpha-ketoglutarate-dependent dioxygenase, by 5-ethynyluracil.
Biochemistry 1993;32(50):14034-42.
1993: L D Thornburg; M T Lai; Pete Wishnok; JoAnne Stubbe
A non-heme iron protein with heme tendencies: an investigation of the substrate specificity of thymine hydroxylase.
Biochemistry 1993;32(50):14023-33.
1993: S Salowe; J Martin Bollinger; M Ator; JoAnne Stubbe; J McCraken; Jack Peisach; M C Samano; Morris J Robins
Alternative model for mechanism-based inhibition of Escherichia coli ribonucleotide reductase by 2'-azido-2'-deoxyuridine 5'-diphosphate.
Biochemistry 1993;32(47):12749-60.
1993: S Booker; JoAnne Stubbe
Cloning, sequencing, and expression of the adenosylcobalamin-dependent ribonucleotide reductase from Lactobacillus leichmannii.
Proceedings of the National Academy of Sciences of the United States of America 1993;90(18):8352-6.
1993: T U Gerngross; P Reilly; JoAnne Stubbe; Anthony J Sinskey; O P Peoples
Immunocytochemical analysis of poly-beta-hydroxybutyrate (PHB) synthase in Alcaligenes eutrophus H16: localization of the synthase enzyme at the surface of PHB granules.
Journal of bacteriology 1993;175(16):5289-93.
1993: S Booker; J Broderick; JoAnne Stubbe
Ribonucleotide reductases: radical enzymes with suicidal tendencies.
Biochemical Society transactions 1993;21 ( Pt 3)(3):727-30.
1993: L Worth; B L Frank; D F Christner; M J Absalon; JoAnne Stubbe; John W Kozarich
Isotope effects on the cleavage of DNA by bleomycin: mechanism and modulation.
Biochemistry 1993;32(10):2601-9.
1993: JoAnne Stubbe; S Booker; J Broderick; S S Mao; M Ator; G Harris; G Ashley; A E Linn; G X Yu
Ribonucleotide reductases: radical enzymes with suicidal tendencies.
Nucleic acids symposium series 1993;(29):107.
1992: S S Mao; G X Yu; D Chalfoun; JoAnne Stubbe
Characterization of C439SR1, a mutant of Escherichia coli ribonucleotide diphosphate reductase: evidence that C439 is a residue essential for nucleotide reduction and C439SR1 is a protein possessing novel thioredoxin-like activity.
Biochemistry 1992;31(40):9752-9.
1992: S S Mao; T P Holler; J Martin Bollinger; G X Yu; M I Johnston; JoAnne Stubbe
Interaction of C225SR1 mutant subunit of ribonucleotide reductase with R2 and nucleoside diphosphates: tales of a suicidal enzyme.
Biochemistry 1992;31(40):9744-51.
1992: S S Mao; T P Holler; G X Yu; J Martin Bollinger; S Booker; M I Johnston; JoAnne Stubbe
A model for the role of multiple cysteine residues involved in ribonucleotide reduction: amazing and still confusing.
Biochemistry 1992;31(40):9733-43.
1992: M J Absalon; C R Krishnamoorthy; G McGall; John W Kozarich; JoAnne Stubbe
Bleomycin mediated degradation of DNA-RNA hybrids does not involve C-1' chemistry.
Nucleic acids research 1992;20(16):4179-85.
1992: E Meyer; N J Leonard; B Bhat; JoAnne Stubbe; J M Smith
Purification and characterization of the purE, purK, and purC gene products: identification of a previously unrecognized energy requirement in the purine biosynthetic pathway.
Biochemistry 1992;31(21):5022-32.
1991: J Martin Bollinger; Dale E Edmondson; Boi Hanh Huynh; J Filley; J R Norton; JoAnne Stubbe
Mechanism of assembly of the tyrosyl radical-dinuclear iron cluster cofactor of ribonucleotide reductase.
Science (New York, N.Y.) 1991;253(5017):292-8.
1991: C H Baker; J Banzon; J Martin Bollinger; JoAnne Stubbe; V Samano; Morris J Robins; B Lippert; E Jarvi; R Resvick
2'-Deoxy-2'-methylenecytidine and 2'-deoxy-2',2'-difluorocytidine 5'-diphosphates: potent mechanism-based inhibitors of ribonucleotide reductase.
Journal of medicinal chemistry 1991;34(6):1879-84.
1991: Lizzy S Kappen; Irving H Goldberg; B L Frank; L Worth; D F Christner; John W Kozarich; JoAnne Stubbe
Neocarzinostatin-induced hydrogen atom abstraction from C-4' and C-5' of the T residue at a d(GT) step in oligonucleotides: shuttling between deoxyribose attack sites based on isotope selection effects.
Biochemistry 1991;30(8):2034-42.
1991: A Mazumder; John Alan Gerlt; M J Absalon; JoAnne Stubbe; Richard P Cunningham; J Withka; Philip H Bolton
Stereochemical studies of the beta-elimination reactions at aldehydic abasic sites in DNA: endonuclease III from Escherichia coli, sodium hydroxide, and Lys-Trp-Lys.
Biochemistry 1991;30(4):1119-26.
1990: JoAnne Stubbe
Ribonucleotide reductases: amazing and confusing.
The Journal of biological chemistry 1990;265(10):5329-32.
1990: Y S Cheng; Y Shen; J Rudolph; M Stern; JoAnne Stubbe; K A Flannigan; J M Smith
Glycinamide ribonucleotide synthetase from Escherichia coli: cloning, overproduction, sequencing, isolation, and characterization.
Biochemistry 1990;29(1):218-27.
1990: JoAnne Stubbe
Ribonucleotide reductases.
Advances in enzymology and related areas of molecular biology 1990;63():349-419.
1989: John W Kozarich; L Worth; B L Frank; D F Christner; Dana E Vanderwall; JoAnne Stubbe
Sequence-specific isotope effects on the cleavage of DNA by bleomycin.
Science (New York, N.Y.) 1989;245(4924):1396-9.
1989: F J Schendel; E Mueller; JoAnne Stubbe; A Shiau; J M Smith
Formylglycinamide ribonucleotide synthetase from Escherichia coli: cloning, sequencing, overproduction, isolation, and characterization.
Biochemistry 1989;28(6):2459-71.
1989: S S Mao; M I Johnston; J Martin Bollinger; JoAnne Stubbe
Mechanism-based inhibition of a mutant Escherichia coli ribonucleotide reductase (cysteine-225----serine) by its substrate CDP.
Proceedings of the National Academy of Sciences of the United States of America 1989;86(5):1485-9.
1989: JoAnne Stubbe
Protein radical involvement in biological catalysis?
Annual review of biochemistry 1989;58():257-85.
1988: G W Ashley; G Harris; JoAnne Stubbe
Inactivation of the ribonucleoside triphosphate reductase from Lactobacillus leichmannii by 2'-chloro-2'-deoxyuridine 5'-triphosphate: a 3'-2' hydrogen transfer during the formation of 3'-keto-2'-deoxyuridine 5'-triphosphate.
Biochemistry 1988;27(20):7841-5.
1988: G W Ashley; G Harris; JoAnne Stubbe
Inactivation of the Lactobacillus leichmannii ribonucleoside triphosphate reductase by 2'-chloro-2'-deoxyuridine 5'-triphosphate: stoichiometry of inactivation, site of inactivation, and mechanism of the protein chromophore formation.
Biochemistry 1988;27(12):4305-10.
1988: JoAnne Stubbe
Radicals in biological catalysis.
Biochemistry 1988;27(11):3893-900.
1988: F J Schendel; Y S Cheng; J D Otvos; S Wehrli; JoAnne Stubbe
Characterization and chemical properties of phosphoribosylamine, an unstable intermediate in the de novo purine biosynthetic pathway.
Biochemistry 1988;27(7):2614-23.
1987: A N Lin; G W Ashley; JoAnne Stubbe
Location of the redox-active thiols of ribonucleotide reductase: sequence similarity between the Escherichia coli and Lactobacillus leichmannii enzymes.
Biochemistry 1987;26(22):6905-9.
1987: S P Salowe; M A Ator; JoAnne Stubbe
Products of the inactivation of ribonucleoside diphosphate reductase from Escherichia coli with 2'-azido-2'-deoxyuridine 5'-diphosphate.
Biochemistry 1987;26(12):3408-16.
1987: G Harris; G W Ashley; M J Robins; R L Tolman; JoAnne Stubbe
2'-Deoxy-2'-halonucleotides as alternate substrates and mechanism-based inactivators of Lactobacillus leichmannii ribonucleotide reductase.
Biochemistry 1987;26(7):1895-902.
1987: Y S Cheng; M Murray; F Schendel; J Otvos; S Wehrli; JoAnne Stubbe
Chemical characterization of phosphoribosylamine, a substrate for newly discovered trifunctional protein containing glycineamide ribonucleotide synthetase activity.
Advances in enzyme regulation 1987;26():319-33.
1987: A I Lin; G W Ashley; JoAnne Stubbe
Lactobacillus leichmannii and Escherichia coli ribonucleotide reductases: chemical and structural similarities.
Cold Spring Harbor symposia on quantitative biology 1987;52():587-96.
1986: S Ajmera; J C Wu; L Worth; L E Rabow; JoAnne Stubbe; John W Kozarich
DNA degradation by bleomycin: evidence for 2'R-proton abstraction and for C-O bond cleavage accompanying base propenal formation.
Biochemistry 1986;25(21):6586-92.
1986: J L Schrimsher; F J Schendel; JoAnne Stubbe
Isolation of a multifunctional protein with aminoimidazole ribonucleotide synthetase, glycinamide ribonucleotide synthetase, and glycinamide ribonucleotide transformylase activities: characterization of aminoimidazole ribonucleotide synthetase.
Biochemistry 1986;25(15):4356-65.
1986: J L Schrimsher; F J Schendel; JoAnne Stubbe; J M Smith
Purification and characterization of aminoimidazole ribonucleotide synthetase from Escherichia coli.
Biochemistry 1986;25(15):4366-71.
1986: F J Schendel; JoAnne Stubbe
Substrate specificity of formylglycinamidine synthetase.
Biochemistry 1986;25(8):2256-64.
1986: G W Ashley; G Harris; JoAnne Stubbe
The mechanism of Lactobacillus leichmannii ribonucleotide reductase. Evidence for 3' carbon-hydrogen bond cleavage and a unique role for coenzyme B12.
The Journal of biological chemistry 1986;261(9):3958-64.
1986: M A Ator; JoAnne Stubbe; T Spector
Mechanism of ribonucleotide reductase from herpes simplex virus type 1. Evidence for 3' carbon-hydrogen bond cleavage and inactivation by nucleotide analogs.
The Journal of biological chemistry 1986;261(8):3595-9.
1986: S P Salowe; JoAnne Stubbe
Cloning, overproduction, and purification of the B2 subunit of ribonucleoside-diphosphate reductase.
Journal of bacteriology 1986;165(2):363-6.
1985: Susan Colette Daubner; J L Schrimsher; F J Schendel; M Young; S Henikoff; D Patterson; JoAnne Stubbe; S J Benkovic
A multifunctional protein possessing glycinamide ribonucleotide synthetase, glycinamide ribonucleotide transformylase, and aminoimidazole ribonucleotide synthetase activities in de novo purine biosynthesis.
Biochemistry 1985;24(25):7059-62.
1985: M A Ator; JoAnne Stubbe
Mechanism of inactivation of Escherichia coli ribonucleotide reductase by 2'-chloro-2'-deoxyuridine 5'-diphosphate: evidence for generation of a 2'-deoxy-3'-ketonucleotide via a net 1,2 hydrogen shift.
Biochemistry 1985;24(25):7214-21.
1985: G W Ashley; JoAnne Stubbe
Current ideas on the chemical mechanism of ribonucleotide reductases.
Pharmacology & therapeutics 1985;30(3):301-29.
1984: G Harris; M Ator; JoAnne Stubbe
Mechanism of inactivation of Escherichia coli and Lactobacillus leichmannii ribonucleotide reductases by 2'-chloro-2'-deoxynucleotides: evidence for generation of 2-methylene-3(2H)-furanone.
Biochemistry 1984;23(22):5214-25.
1983: JoAnne Stubbe; M Ator; T Krenitsky
Mechanism of ribonucleoside diphosphate reductase from Escherichia coli. Evidence for 3'-C--H bond cleavage.
The Journal of biological chemistry 1983;258(3):1625-31.