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Kouhei Tsumoto

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Toshitaka Matsui; Shusuke Nambu; Yukari Ono; Kouhei Tsumoto; Celia W Goulding; Masao Ikeda-Saito
Heme Degradation by Staphylococcus aureus IsdG and IsdI Liberates Formaldehyde Rather Than Carbon Monoxide.
Toshiaki Takei; Kouhei Tsumoto; Takuya Ueda; Kazumori Yazaki; Masakuni Yoshino; Kazuya Hasegawa; Katsumi Imada; Shuichi Kojima; Yukino Kuriki; Keiichi Namba; Tsunetomo Takei; Kin-ichiro Miura
Effects of Chain Length of an Amphipathic Polypeptide Carrying the Repeated Amino Acid Sequence (LETLAKA)n on α-Helix and Fibrous Assembly Formation.
Akihiro Kobe; Tomoya Mitani; Shinya Tashiro; Jose M M Caaveiro; Daisuke Kajihara; Masato Kikkawa; Kouhei Tsumoto
Incorporation of rapid thermodynamic data in fragment-based drug discovery.

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2013: Toshitaka Matsui; Shusuke Nambu; Yukari Ono; Kouhei Tsumoto; Celia W Goulding; Masao Ikeda-Saito
Heme Degradation by Staphylococcus aureus IsdG and IsdI Liberates Formaldehyde Rather Than Carbon Monoxide.
Biochemistry 2013;52(18):3025-7.
2013: Toshiaki Takei; Kouhei Tsumoto; Takuya Ueda; Kazumori Yazaki; Masakuni Yoshino; Kazuya Hasegawa; Katsumi Imada; Shuichi Kojima; Yukino Kuriki; Keiichi Namba; Tsunetomo Takei; Kin-ichiro Miura
Effects of Chain Length of an Amphipathic Polypeptide Carrying the Repeated Amino Acid Sequence (LETLAKA)n on α-Helix and Fibrous Assembly Formation.
Biochemistry 2013;52(16):2810-20.
2013: Akihiro Kobe; Tomoya Mitani; Shinya Tashiro; Jose M M Caaveiro; Daisuke Kajihara; Masato Kikkawa; Kouhei Tsumoto
Incorporation of rapid thermodynamic data in fragment-based drug discovery.
Journal of medicinal chemistry 2013;56(5):2155-9.
2013: Chihoko Yoshimura; Takamitsu Miyafusa; Kouhei Tsumoto
Identification of small-molecule inhibitors of the human S100B-p53 interaction and evaluation of their activity in human melanoma cells.
Bioorganic & medicinal chemistry 2013;21(5):1109-15.
2013: Kenichi Harada; Takamitsu Miyafusa; Atsushi Nakagawa; Yoshiharu Toyama; Kouhei Tsumoto; Takashi Ueno; Eiki Yamashita; Shigeki Takeda
Crystal structure of the C-terminal domain of Mu phage central spike and functions of bound calcium ion.
Biochimica et biophysica acta 2013;1834(1):284-91.
2012: Emiko Sano; Hisashi Tadakuma; Toshiaki Takei; Shinnya Tashiro; Takuya Ueda; Kouhei Tsumoto
Type 1 IFN inhibits the growth factor deprived apoptosis of cultured human aortic endothelial cells and protects the cells from chemically induced oxidative cytotoxicity.
Journal of cellular biochemistry 2012;113(12):3823-34.
2012: Shinnya Tashiro; Kouhei Tsumoto; Emiko Sano
Establishment of a microcarrier culture system with serial sub-cultivation for functionally active human endothelial cells.
Journal of biotechnology 2012;160(3-4):202-13.
2012: Shota Kudo; Tadashi Matsuura; Takamitsu Miyafusa; Yukio Sudou; Jose M M Caaveiro; Shuichiro Goda; Takao Hamakubo; Keisuke Ishii; Tatsuhiko Kodama; Kouhei Tsumoto
Structural and thermodynamic characterization of the self-adhesive properties of human P-cadherin.
Molecular bioSystems 2012;8(8):2050-3.
2012: Mihoko Ui; Takehiko Wada; Yasuyuki Araki; Sumire Endo; Toshiaki Takei; Yoshikazu Tanaka; Kouhei Tsumoto; Kazushi Kinbara
Application of photoactive yellow protein as a photoresponsive module for controlling hemolytic activity of staphylococcal α-hemolysin.
Chemical communications (Cambridge, England) 2012;48(39):4737-9.
2012: Ryota Abe; Jose M M Caaveiro; Hiroko Kozuka-Hata; Masaaki Oyama; Kouhei Tsumoto
Mapping ultra-weak protein-protein interactions between heme transporters of Staphylococcus aureus.
The Journal of biological chemistry 2012;287(20):16477-87.
2012: Takamitsu Miyafusa; Yoshikazu Tanaka; Jose M M Caaveiro; Kouhei Tsumoto
Crystal structure of the enzyme CapF of Staphylococcus aureus reveals a unique architecture composed of two functional domains.
The Biochemical journal 2012;443(3):671-80.
2012: Hiroko Kozuka-Hata; Yukiko Nasu-Nishimura; Tetsu Akiyama; Hiroko Ao-Kondo; Ryo Koyama-Nasu; Kouhei Tsumoto; Masaaki Oyama
Phosphoproteome of human glioblastoma initiating cells reveals novel signaling regulators encoded by the transcriptome.
PloS one 2012;7(8):e43398.
2011: Takeaki Kawai; Ryota Abe; Jose M M Caaveiro; Toyomasa Katagiri; Kouhei Tsumoto
Catalytic activity of MsbA reconstituted in nanodisc particles is modulated by remote interactions with the bilayer.
FEBS letters 2011;585(22):3533-7.
2011: Yoshitaka Moriwaki; Yoshikazu Tanaka; Jose M M Caaveiro; Itaru Hamachi; Hiroshi Tsutsumi; Kouhei Tsumoto
Molecular basis of recognition of antibacterial porphyrins by heme-transporter IsdH-NEAT3 of Staphylococcus aureus.
Biochemistry 2011;50(34):7311-20.
2011: Satoru Nagatoishi; Noburu Isono; Kouhei Tsumoto; Naoki Sugimoto
Hydration is required in DNA G-quadruplex-protein binding.
Chembiochem : a European journal of chemical biology 2011;12(12):1822-6.
2011: Satoru Nagatoishi; Noburu Isono; Kouhei Tsumoto; Naoki Sugimoto
Loop residues of thrombin-binding DNA aptamer impact G-quadruplex stability and thrombin binding.
Biochimie 2011;93(8):1231-8.
2011: Toru Suzuki; Minsoo Kim; Hiroko Kozuka-Hata; Masato Watanabe; Masaaki Oyama; Kouhei Tsumoto; Tadashi Yamamoto
Monoubiquitination of Tob/BTG family proteins competes with degradation-targeting polyubiquitination.
Biochemical and biophysical research communications 2011;409(1):70-4.
2011: Saeko Yanaka; Emiko Sano; Norio Naruse; Kin-ichiro Miura; Mutsumi Futatsumori-Sugai; Jose M M Caaveiro; Kouhei Tsumoto
Non-core region modulates interleukin-11 signaling activity: generation of agonist and antagonist variants.
The Journal of biological chemistry 2011;286(10):8085-93.
2011: Mihoko Ui; Yoshikazu Tanaka; Takeshi Tsumuraya; Ikuo Fujii; Masayuki Inoue; Masahiro Hirama; Kouhei Tsumoto
Structural and energetic hot-spots for the interaction between a ladder-like polycyclic ether and the anti-ciguatoxin antibody 10C9Fab.
Molecular bioSystems 2011;7(3):793-8.
2010: Mihoko Ui; Kouhei Tsumoto
An approach to rational ligand-design based on a thermodynamic analysis.
Recent patents on biotechnology 2010;4(3):183-8.
2010: Yoshiyuki Sasajima; Ryohei Iwasaki; Kouhei Tsumoto; Izumi Kumagai; Masaki Ihara; Hiroshi Ueda
Expression of antibody variable region-human alkaline phosphatase fusion proteins in mammalian cells.
Journal of immunological methods 2010;361(1-2):57-63.
2010: Shun Kumano; Koji Iida; Kenji Ishihara; Michio Murakoshi; Kouhei Tsumoto; Katsuhisa Ikeda; Izumi Kumagai; Toshimitsu Kobayashi; Hiroshi Wada
Salicylate-induced translocation of prestin having mutation in the GTSRH sequence to the plasma membrane.
FEBS letters 2010;584(11):2327-32.
2010: Tsutomu Arakawa; Mutsumi Futatsumori-Sugai; Kouhei Tsumoto; Yoshiko Kita; Haruna Sato; Daisuke Ejima
MEP HyperCel chromatography II: binding, washing and elution.
Protein expression and purification 2010;71(2):168-73.
2010: Saeko Yanaka; Motonori Kudou; Yoshikazu Tanaka; Takumi Sasaki; Sumiyo Takemoto; Atsuko Sakata; Yukio Hattori; Tomoyuki Koshi; Shiro Futaki; Kouhei Tsumoto; Toshihiro Nakashima
Contribution of the flexible loop region to the function of staphylococcal enterotoxin B.
Protein engineering, design & selection : PEDS 2010;23(5):415-21.
2010: Ryota Abe; Jose M M Caaveiro; Motonori Kudou; Kouhei Tsumoto
Solubilization of membrane proteins with novel N-acylamino acid detergents.
Molecular bioSystems 2010;6(4):677-9.
2010: Ryosuke Yumioka; Kouhei Tsumoto; Tsutomu Arakawa; Daisuke Ejima
Screening of effective column rinse solvent for Protein-A chromatography.
Protein expression and purification 2010;70(2):218-23.
2010: Akiko Yokota; Kouhei Tsumoto; Mitsunori Shiroishi; Takeshi Nakanishi; Hidemasa Kondo; Izumi Kumagai
Contribution of asparagine residues to the stabilization of a proteinaceous antigen-antibody complex, HyHEL-10-hen egg white lysozyme.
The Journal of biological chemistry 2010;285(10):7686-96.
2010: Nozomi Yokoo; Takanari Togashi; Mitsuo Umetsu; Kouhei Tsumoto; Takamitsu Hattori; Takeshi Nakanishi; Satoshi Ohara; Seiichi Takami; Takashi Naka; Hiroya Abe; Izumi Kumagai; Tadafumi Adschiri
Direct and selective immobilization of proteins by means of an inorganic material-binding peptide: discussion on functionalization in the elongation to material-binding peptide.
The journal of physical chemistry. B 2010;114(1):480-6.
2010: Satoru Nagatoishi; Yoshikazu Tanaka; Motonori Kudou; Kouhei Tsumoto
Temperature and salt concentration alter base-sequence selectivity of a duplex DNA-binding protein.
Molecular bioSystems 2010;6(1):98-101.
2010: Mutsumi Futatsumori-Sugai; Kouhei Tsumoto
Signal peptide design for improving recombinant protein secretion in the baculovirus expression vector system.
Biochemical and biophysical research communications 2010;391(1):931-5.
2009: Kouhei Tsumoto; Mihoko Ui
[Rational fragment-design method based on a thermodynamic analysis].
Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan 2009;129(11):1311-7.
2009: Mutsumi Futatsumori-Sugai; Ryota Abe; Masato Watanabe; Motonori Kudou; Tadashi Yamamoto; Daisuke Ejima; Tsutomu Arakawa; Kouhei Tsumoto
Utilization of Arg-elution method for FLAG-tag based chromatography.
Protein expression and purification 2009;67(2):148-55.
2009: Satoru Nagatoishi; Yoshikazu Tanaka; Motonori Kudou; Kouhei Tsumoto
The interaction of hyperthermophilic TATA-box binding protein with single-stranded DNA is entropically favorable and exhibits a large negative heat capacity change at high salt concentration.
Molecular bioSystems 2009;5(9):957-61.
2009: Ryota Abe; Motonori Kudou; Yoshikazu Tanaka; Tsutomu Arakawa; Kouhei Tsumoto
Immobilized metal affinity chromatography in the presence of arginine.
Biochemical and biophysical research communications 2009;381(3):306-10.
2009: Nobuchika Suzuki; Kouhei Tsumoto; Nicole Hajicek; Kenji Daigo; Reiko Tokita; Shiro Minami; Tatsuhiko Kodama; Takao Hamakubo; Tohru Kozasa
Activation of leukemia-associated RhoGEF by Galpha13 with significant conformational rearrangements in the interface.
The Journal of biological chemistry 2009;284(8):5000-9.
2009: Kou Okuro; Kazushi Kinbara; Kouhei Tsumoto; Noriyuki Ishii; Takuzo Aida
Molecular glues carrying multiple guanidinium ion pendants via an oligoether spacer: stabilization of microtubules against depolymerization.
Journal of the American Chemical Society 2009;131(5):1626-7.
2008: Sou Sakamoto; Yoshikazu Tanaka; Isao Tanaka; Toshiaki Takei; Jian Yu; Makoto Kuroda; Min Yao; Toshiko Ohta; Kouhei Tsumoto
Electron microscopy and computational studies of Ebh, a giant cell-wall-associated protein from Staphylococcus aureus.
Biochemical and biophysical research communications 2008;376(2):261-6.
2008: Masato Watanabe; Yoshikazu Tanaka; Ayuko Suenaga; Makoto Kuroda; Min Yao; Nobuhisa Watanabe; Fumio Arisaka; Toshiko Ohta; Isao Tanaka; Kouhei Tsumoto
Structural basis for multimeric heme complexation through a specific protein-heme interaction: the case of the third neat domain of IsdH from Staphylococcus aureus.
The Journal of biological chemistry 2008;283(42):28649-59.
2008: Makoto Nakakido; Yoshikazu Tanaka; Mariko Mitsuhori; Motonori Kudou; Daisuke Ejima; Tsutomu Arakawa; Kouhei Tsumoto
Structure-based analysis reveals hydration changes induced by arginine hydrochloride.
Biophysical chemistry 2008;137(2-3):105-9.
2008: Makoto Kuroda; Yoshikazu Tanaka; Ryo Aoki; Deng Shu; Kouhei Tsumoto; Toshiko Ohta
Staphylococcus aureus giant protein Ebh is involved in tolerance to transient hyperosmotic pressure.
Biochemical and biophysical research communications 2008;374(2):237-41.
2008: Takahiro Sogo; Masahiro Kawahara; Kouhei Tsumoto; Izumi Kumagai; Hiroshi Ueda; Teruyuki Nagamune
Selective expansion of genetically modified T cells using an antibody/interleukin-2 receptor chimera.
Journal of immunological methods 2008;337(1):16-23.
2008: Daizo Hamada; Kouhei Tsumoto; Makoto Sawara; Naoki Tanaka; Kumiko Nakahira; Kentaro Shiraki; Itaru Yanagihara
Effect of an amyloidogenic sequence attached to yellow fluorescent protein.
Proteins 2008;72(3):811-21.
2008: Mihoko Ui; Yoshikazu Tanaka; Takeshi Tsumuraya; Ikuo Fujii; Masayuki Inoue; Masahiro Hirama; Kouhei Tsumoto
How protein recognizes ladder-like polycyclic ethers. Interactions between ciguatoxin (CTX3C) fragments and its specific antibody 10C9.
The Journal of biological chemistry 2008;283(28):19440-7.
2008: Makoto Nakakido; Yoshikazu Tanaka; Masaaki Sokabe; Kouhei Tsumoto
Thermodynamic analysis reveals that GTP binding affects the interaction between the alpha- and gamma-subunits of translation initiation factor 2.
Biochemical and biophysical research communications 2008;371(4):596-9.
2008: Takamitsu Miyafusa; Yoshikazu Tanaka; Makoto Kuroda; Toshiko Ohta; Kouhei Tsumoto
Expression, purification, crystallization and preliminary diffraction analysis of CapF, a capsular polysaccharide-synthesis enzyme from Staphylococcus aureus.
Acta crystallographica. Section F, Structural biology and crystallization communications 2008;64(Pt 6):512-5.
2008: Kouhei Tsumoto; Akiko Yokota; Yoshikazu Tanaka; Mihoko Ui; Takeshi Tsumuraya; Ikuo Fujii; Izumi Kumagai; Yoko Nagumo; Hiroki Oguri; Masayuki Inoue; Masahiro Hirama
Critical contribution of aromatic rings to specific recognition of polyether rings. The case of ciguatoxin CTX3C-ABC and its specific antibody 1C49.
The Journal of biological chemistry 2008;283(18):12259-66.
2008: Yoshikazu Tanaka; Sou Sakamoto; Makoto Kuroda; Shuichiro Goda; Yong-Gui Gao; Kouhei Tsumoto; Yuzuru Hiragi; Min Yao; Nobuhisa Watanabe; Toshiko Ohta; Isao Tanaka
A helical string of alternately connected three-helix bundles for the cell wall-associated adhesion protein Ebh from Staphylococcus aureus.
Structure (London, England : 1993) 2008;16(3):488-96.
2008: Takamitsu Hattori; Mitsuo Umetsu; Takeshi Nakanishi; Kouhei Tsumoto; Satoshi Ohara; Hiroya Abe; Makio Naito; Ryutaro Asano; Tadafumi Adschiri; Izumi Kumagai
Grafting of material-binding function into antibodies Functionalization by peptide grafting.
Biochemical and biophysical research communications 2008;365(4):751-7.
2008: Koki Makabe; Takeshi Nakanishi; Kouhei Tsumoto; Yoshikazu Tanaka; Hidemasa Kondo; Mitsuo Umetsu; Yukiko Sone; Ryutaro Asano; Izumi Kumagai
Thermodynamic consequences of mutations in vernier zone residues of a humanized anti-human epidermal growth factor receptor murine antibody, 528.
The Journal of biological chemistry 2008;283(2):1156-66.
2008: Tsutomu Arakawa; Daisuke Ejima; Kouhei Tsumoto; Pete Gagnon
[Solvent modulation of chromatography].
Seikagaku. The Journal of Japanese Biochemical Society 2008;80(1):45-51.
2007: T Arakawa; Kouhei Tsumoto; Y Kita; B Chang; Daisuke Ejima
Biotechnology applications of amino acids in protein purification and formulations.
Amino acids 2007;33(4):587-605.
2007: Makoto Nakakido; Yoshikazu Tanaka; Kouhei Tsumoto
The N-terminal domain of elastin-binding protein of Staphylococcus aureus changes its secondary structure in a membrane-mimetic environment.
Journal of biochemistry 2007;142(2):131-4.
2007: Yoshikazu Tanaka; Takashi Sasaki; Izumi Kumagai; Yoshiaki Yasutake; Min Yao; Isao Tanaka; Kouhei Tsumoto
Molecular properties of two proteins homologous to PduO-type ATP:cob(I)alamin adenosyltransferase from Sulfolobus tokodaii.
Proteins 2007;68(2):446-57.
2007: Tsutomu Arakawa; Kouhei Tsumoto; Kazuo Nagase; Daisuke Ejima
The effects of arginine on protein binding and elution in hydrophobic interaction and ion-exchange chromatography.
Protein expression and purification 2007;54(1):110-6.
2007: Kouhei Tsumoto; Daisuke Ejima; Anna Senczuk; Yoshiko Kita; Tsutomu Arakawa
Effects of salts on protein-surface interactions: applications for column chromatography.
Journal of pharmaceutical sciences 2007;96(7):1677-90.
2007: Kouhei Tsumoto; Daisuke Ejima; Kazuo Nagase; Tsutomu Arakawa
Arginine improves protein elution in hydrophobic interaction chromatography. The cases of human interleukin-6 and activin-A.
Journal of chromatography. A 2007;1154(1-2):81-6.
2007: Hideki Watanabe; Kouhei Tsumoto; Seiichi Taguchi; Koichi Yamashita; Yoshiharu Doi; Yoshiyuki Nishimiya; Hidemasa Kondo; Mitsuo Umetsu; Izumi Kumagai
A human antibody fragment with high affinity for biodegradable polymer film.
Bioconjugate chemistry 2007;18(3):645-51.
2007: Masahiro Kawahara; Hiroshi Ueda; Kouhei Tsumoto; Izumi Kumagai; Teruyuki Nagamune
Mimicry of erythropoietin and interleukin-6 signalling by an antibody/cytokine receptor chimera in murine myeloid 32D cells.
Journal of biochemistry 2007;141(4):563-71.
2007: Tsutomu Arakawa; Daisuke Ejima; Kouhei Tsumoto; Noriyuki Obeyama; Yoshikazu Tanaka; Yoshiko Kita; Serge N Timasheff
Suppression of protein interactions by arginine: a proposed mechanism of the arginine effects.
Biophysical chemistry 2007;127(1-2):1-8.
2007: Tsutomu Arakawa; Daisuke Ejima; Kouhei Tsumoto; Matsujiro Ishibashi; Masao Tokunaga
Improved performance of column chromatography by arginine: dye-affinity chromatography.
Protein expression and purification 2007;52(2):410-4.
2007: Mitsunori Shiroishi; Kouhei Tsumoto; Yoshikazu Tanaka; Akiko Yokota; Takeshi Nakanishi; Hidemasa Kondo; Izumi Kumagai
Structural consequences of mutations in interfacial Tyr residues of a protein antigen-antibody complex. The case of HyHEL-10-HEL.
The Journal of biological chemistry 2007;282(9):6783-91.
2007: Daisuke Ejima; Kouhei Tsumoto; Harumi Fukada; Ryosuke Yumioka; Kazuo Nagase; Tsutomu Arakawa; John Philo
Effects of acid exposure on the conformation, stability, and aggregation of monoclonal antibodies.
Proteins 2007;66(4):954-62.
2007: Satoru Nagatoishi; Yoshikazu Tanaka; Kouhei Tsumoto
Circular dichroism spectra demonstrate formation of the thrombin-binding DNA aptamer G-quadruplex under stabilizing-cation-deficient conditions.
Biochemical and biophysical research communications 2007;352(3):812-7.
2006: Masahiro Kawahara; Yuko Ogo; Kouhei Tsumoto; Izumi Kumagai; Hiroshi Ueda; Teruyuki Nagamune
Growth control of hybridoma cells with an artificially induced EpoR-gp130 heterodimer.
Cytotechnology 2006;52(3):171-9.
2006: Tsutomu Arakawa; Daisuke Ejima; Yoshiko Kita; Kouhei Tsumoto
Small molecule pharmacological chaperones: From thermodynamic stabilization to pharmaceutical drugs.
Biochimica et biophysica acta 2006;1764(11):1677-87.
2006: Ryutaro Asano; Yukiko Sone; Koki Makabe; Kouhei Tsumoto; Hiroki Hayashi; Yu Katayose; Michiaki Unno; Toshio Kudo; Izumi Kumagai
Humanization of the bispecific epidermal growth factor receptor x CD3 diabody and its efficacy as a potential clinical reagent.
Clinical cancer research : an official journal of the American Association for Cancer Research 2006;12(13):4036-42.
2006: Daisuke Ejima; Kunio Ono; Kouhei Tsumoto; Tsutomu Arakawa; Yuzuru Eto
A novel "reverse screening" to identify refolding additives for activin-A.
Protein expression and purification 2006;47(1):45-51.
2006: Kouhei Tsumoto
[Dissection of specificity and affinity of an antibody for its specific antigen].
Seikagaku. The Journal of Japanese Biochemical Society 2006;78(2):93-109.
2006: Tsutomu Arakawa; Yoshiko Kita; Daisuke Ejima; Kouhei Tsumoto; Harumi Fukada
Aggregation suppression of proteins by arginine during thermal unfolding.
Protein and peptide letters 2006;13(9):921-7.
2005: Yoshikazu Tanaka; Kouhei Tsumoto; Eiki Tanabe; Yoshiaki Yasutake; Naoki Sakai; Min Yao; Isao Tanaka; Izumi Kumagai
Crystal structure of the hypothetical protein ST2072 from Sulfolobus tokodaii.
Proteins 2005;61(4):1127-31.
2005: Daisuke Ejima; Ryosuke Yumioka; Tsutomu Arakawa; Kouhei Tsumoto
Arginine as an effective additive in gel permeation chromatography.
Journal of chromatography. A 2005;1094(1-2):49-55.
2005: Daisuke Ejima; Ryosuke Yumioka; Kouhei Tsumoto; Tsutomu Arakawa
Effective elution of antibodies by arginine and arginine derivatives in affinity column chromatography.
Analytical biochemistry 2005;345(2):250-7.
2005: Kouhei Tsumoto; Daisuke Ejima; Yoshiko Kita; Tsutomu Arakawa
Review: Why is arginine effective in suppressing aggregation?
Protein and peptide letters 2005;12(7):613-9.
2005: Mitsuo Umetsu; Kouhei Tsumoto; Shigeki Nitta; Tadafumi Adschiri; Daisuke Ejima; Tsutomu Arakawa; Izumi Kumagai
Nondenaturing solubilization of beta2 microglobulin from inclusion bodies by L-arginine.
Biochemical and biophysical research communications 2005;328(1):189-97.
2005: Koki Makabe; Ryutaro Asano; Takahiko Ito; Kouhei Tsumoto; Toshio Kudo; Izumi Kumagai
Tumor-directed lymphocyte-activating cytokines: refolding-based preparation of recombinant human interleukin-12 and an antibody variable domain-fused protein by additive-introduced stepwise dialysis.
Biochemical and biophysical research communications 2005;328(1):98-105.
2004: Yoshikazu Tanaka; Kouhei Tsumoto; Mitsuo Umetsu; Takeshi Nakanishi; Yoshiaki Yasutake; Naoki Sakai; Min Yao; Isao Tanaka; Tsutomu Arakawa; Izumi Kumagai
Structural evidence for guanidine-protein side chain interactions: crystal structure of CutA from Pyrococcus horikoshii in 3 M guanidine hydrochloride.
Biochemical and biophysical research communications 2004;323(1):185-91.
2004: Kouhei Tsumoto; Mitsuo Umetsu; Izumi Kumagai; Daisuke Ejima; John Philo; Tsutomu Arakawa
Role of arginine in protein refolding, solubilization, and purification.
Biotechnology progress 2004;20(5):1301-8.
2004: Tsutomu Arakawa; John Philo; Kouhei Tsumoto; Ryosuke Yumioka; Daisuke Ejima
Elution of antibodies from a Protein-A column by aqueous arginine solutions.
Protein expression and purification 2004;36(2):244-8.
2004: Masahiro Kawahara; Shinya Ishii; Kouhei Tsumoto; Izumi Kumagai; Hiroshi Ueda; Teruyuki Nagamune
Reversal of antigen-dependent signaling by two mutations in antibody/receptor chimera: implication of inverse agonism in cytokine receptor superfamily.
Biochemical pharmacology 2004;68(3):539-48.
2004: Yoshikazu Tanaka; Kouhei Tsumoto; Yoshiaki Yasutake; Mitsuo Umetsu; Min Yao; Harumi Fukada; Isao Tanaka; Izumi Kumagai
How oligomerization contributes to the thermostability of an archaeon protein. Protein L-isoaspartyl-O-methyltransferase from Sulfolobus tokodaii.
The Journal of biological chemistry 2004;279(31):32957-67.
2004: Hiroki Hayashi; Ryutaro Asano; Kouhei Tsumoto; Yu Katayose; Masanori Suzuki; Michiaki Unno; Hideaki Kodama; Shin-ichi Takemura; Hiroshi Yoshida; Koki Makabe; Kohzoh Imai; Seiki Matsuno; Izumi Kumagai; Toshio Kudo
A highly effective and stable bispecific diabody for cancer immunotherapy: cure of xenografted tumors by bispecific diabody and T-LAK cells.
Cancer immunology, immunotherapy : CII 2004;53(6):497-509.
2004: Mitsuo Umetsu; Kouhei Tsumoto; Kumar Ashish; Shigeki Nitta; Yoshikazu Tanaka; Tadafumi Adschiri; Izumi Kumagai
Structural characteristics and refolding of in vivo aggregated hyperthermophilic archaeon proteins.
FEBS letters 2004;557(1-3):49-56.
2004: Yoshikazu Tanaka; Kouhei Tsumoto; Takeshi Nakanishi; Yoshiaki Yasutake; Naoki Sakai; Min Yao; Isao Tanaka; Izumi Kumagai
Structural implications for heavy metal-induced reversible assembly and aggregation of a protein: the case of Pyrococcus horikoshii CutA.
FEBS letters 2004;556(1-3):167-74.
2004: Masahiro Kawahara; Hiroshi Ueda; Kouhei Tsumoto; Izumi Kumagai; Teruyuki Nagamune
AMEGA: antigen-mediated genetically modified cell amplification.
Journal of immunological methods 2004;284(1-2):187-94.
2003: Kouhei Tsumoto; Mitsuo Umetsu; Izumi Kumagai; Daisuke Ejima; Tsutomu Arakawa
Solubilization of active green fluorescent protein from insoluble particles by guanidine and arginine.
Biochemical and biophysical research communications 2003;312(4):1383-6.
2003: Hiroshi Ueda; Tomoichi Yokozeki; Ryoichi Arai; Kouhei Tsumoto; Izumi Kumagai; Teruyuki Nagamune
An optimized homogeneous noncompetitive immunoassay based on the antigen-driven enzymatic complementation.
Journal of immunological methods 2003;279(1-2):209-18.
2003: Izumi Kumagai; Yoshiyuki Nishimiya; Hidemasa Kondo; Kouhei Tsumoto
Structural consequences of target epitope-directed functional alteration of an antibody. The case of anti-hen lysozyme antibody, HyHEL-10.
The Journal of biological chemistry 2003;278(27):24929-36.
2003: Kouhei Tsumoto; Mitsuo Umetsu; Hidenari Yamada; Takahiko Ito; Satoru Misawa; Izumi Kumagai
Immobilized oxidoreductase as an additive for refolding inclusion bodies: application to antibody fragments.
Protein engineering 2003;16(7):535-41.
2003: Tsutomu Arakawa; Kouhei Tsumoto
The effects of arginine on refolding of aggregated proteins: not facilitate refolding, but suppress aggregation.
Biochemical and biophysical research communications 2003;304(1):148-52.
2003: Masahiro Kawahara; Hiroshi Ueda; Sumiyo Morita; Kouhei Tsumoto; Izumi Kumagai; Teruyuki Nagamune
Bypassing antibiotic selection: positive screening of genetically modified cells with an antigen-dependent proliferation switch.
Nucleic acids research 2003;31(7):e32.
2003: Mitsuo Umetsu; Kouhei Tsumoto; Masaki Hara; Kumar Ashish; Shuichiro Goda; Tadafumi Adschiri; Izumi Kumagai
How additives influence the refolding of immunoglobulin-folded proteins in a stepwise dialysis system. Spectroscopic evidence for highly efficient refolding of a single-chain Fv fragment.
The Journal of biological chemistry 2003;278(11):8979-87.
2003: Kouhei Tsumoto; Daisuke Ejima; Izumi Kumagai; Tsutomu Arakawa
Practical considerations in refolding proteins from inclusion bodies.
Protein expression and purification 2003;28(1):1-8.
2003: Akiko Yokota; Kouhei Tsumoto; Mitsunori Shiroishi; Hidemasa Kondo; Izumi Kumagai
The role of hydrogen bonding via interfacial water molecules in antigen-antibody complexation. The HyHEL-10-HEL interaction.
The Journal of biological chemistry 2003;278(7):5410-8.
2003: Michihiro Nakamura; Kouhei Tsumoto; Izumi Kumagai; Kazunori Ishimura
A morphologic study of filamentous phage infection of Escherichia coli using biotinylated phages.
FEBS letters 2003;536(1-3):167-72.
2002: Ryutaro Asano; Toshio Kudo; Yukihiro Nishimura; Koki Makabe; Hiroki Hayashi; Masanori Suzuki; Kouhei Tsumoto; Izumi Kumagai
Efficient construction of a diabody using a refolding system: anti-carcinoembryonic antigen recombinant antibody fragment.
Journal of biochemistry 2002;132(6):903-9.
2002: Ryutaro Asano; Toshio Kudo; Koki Makabe; Kouhei Tsumoto; Izumi Kumagai
Antitumor activity of interleukin-21 prepared by novel refolding procedure from inclusion bodies expressed in Escherichia coli.
FEBS letters 2002;528(1-3):70-6.
2002: Kouhei Tsumoto; Satoru Misawa; Yoichi Ohba; Takamasa Ueno; Hideya Hayashi; Nobuhiro Kasai; Hideki Watanabe; Ryutaro Asano; Izumi Kumagai
Inhibition of hepatitis C virus NS3 protease by peptides derived from complementarity-determining regions (CDRs) of the monoclonal antibody 8D4: tolerance of a CDR peptide to conformational changes of a target.
FEBS letters 2002;525(1-3):77-82.
2002: Hideki Watanabe; Kouhei Tsumoto; Ryutaro Asano; Yoshiyuki Nishimiya; Izumi Kumagai
Selection of human antibody fragments on the basis of stabilization of the variable domain in the presence of target antigens.
Biochemical and biophysical research communications 2002;295(1):31-6.
2002: Michihiro Nakamura; Kouhei Tsumoto; Kazunori Ishimura; Izumi Kumagai
The effect of an agglutogen on virus infection: biotinylated filamentous phages and avidin as a model.
FEBS letters 2002;520(1-3):77-80.
2002: Michihiro Nakamura; Kouhei Tsumoto; Kazunori Ishimura; Izumi Kumagai
Detection of biotinylated proteins in polyacrylamide gels using an avidin-fluorescein conjugate.
Analytical biochemistry 2002;304(2):231-5.
2002: Michihiro Nakamura; Kouhei Tsumoto; Kazunori Ishimura; Izumi Kumagai
Phage library panning against cytosolic fraction of cells using quantitative dot blotting assay: application of selected VH to histochemistry.
Journal of immunological methods 2002;261(1-2):65-72.
2002: Masahiro Kawahara; Hiroshi Ueda; Kouhei Tsumoto; Izumi Kumagai; Walt Mahoney; Teruyuki Nagamune
Selection of highly productive mammalian cells based on an inducible growth advantage using an antibody/receptor chimera.
Journal of bioscience and bioengineering 2002;93(4):399-404.
2001: Michihiro Nakamura; Kouhei Tsumoto; Kazunori Ishimura; Izumi Kumagai
A visualization method of filamentous phage infection and phage-derived proteins in Escherichia coli using biotinylated phages.
Biochemical and biophysical research communications 2001;289(1):252-6.
2001: Kazuyoshi Horii; M Saito; Takao Yoda; Kouhei Tsumoto; M Matsushima; Kunihiro Kuwajima; Izumi Kumagai
Contribution of Thr29 to the thermodynamic stability of goat alpha-lactalbumin as determined by experimental and theoretical approaches.
Proteins 2001;45(1):16-29.
2001: Masahiro Kawahara; A Natsume; S Terada; Koichi Kato; Kouhei Tsumoto; Izumi Kumagai; M Miki; Walt Mahoney; Hiroki R Ueda; Teruyuki Nagamune
Replacing factor-dependency with that for lysozyme: affordable culture of IL-6-dependent hybridoma by transfecting artificial cell surface receptor.
Biotechnology and bioengineering 2001;74(5):416-23.
2001: Masahiro Kawahara; Hiroki R Ueda; Kouhei Tsumoto; Izumi Kumagai; Walt Mahoney; Teruyuki Nagamune
A growth signal with an artificially induced erythropoietin receptor-gp130 cytoplasmic domain heterodimer.
Journal of biochemistry 2001;130(2):305-12.
2001: Mitsunori Shiroishi; Akiko Yokota; Kouhei Tsumoto; Hidemasa Kondo; Yoshiyuki Nishimiya; Kazuyoshi Horii; M Matsushima; K Ogasahara; K Yutani; Izumi Kumagai
Structural evidence for entropic contribution of salt bridge formation to a protein antigen-antibody interaction: the case of hen lysozyme-HyHEL-10 Fv complex.
The Journal of biological chemistry 2001;276(25):23042-50.
2001: N Kasai; Kouhei Tsumoto; S Niwa; Satoru Misawa; T Ueno; Hideya Hayashi; Izumi Kumagai
Inhibition of the hepatitis C virus NS3 protease activity by Fv fragment of antibody 8D4.
Biochemical and biophysical research communications 2001;281(2):416-24.
2001: M Nakamura; H Watanabe; Yoshiyuki Nishimiya; Kouhei Tsumoto; K Ishimura; Izumi Kumagai
Panning of a phage VH library using nitrocellulose membranes: application to selection of a human VH library.
Journal of biochemistry 2001;129(2):209-12.
2001: Ryoichi Arai; H Nakagawa; Kouhei Tsumoto; Walt Mahoney; Izumi Kumagai; Hiroki R Ueda; Teruyuki Nagamune
Demonstration of a homogeneous noncompetitive immunoassay based on bioluminescence resonance energy transfer.
Analytical biochemistry 2001;289(1):77-81.
2001: Takao Yoda; M Saito; Munehito Arai; Kazuyoshi Horii; Kouhei Tsumoto; M Matsushima; Izumi Kumagai; Kunihiro Kuwajima
Folding-unfolding of goat alpha-lactalbumin studied by stopped-flow circular dichroism and molecular dynamics simulations.
Proteins 2001;42(1):49-65.
2000: Hiroki R Ueda; Masahiro Kawahara; Takahide Aburatani; Kouhei Tsumoto; K Todokoro; E Suzuki; H Nishimura; PA Schueler; G Winter; Walt Mahoney; Izumi Kumagai; Teruyuki Nagamune
Cell-growth control by monomeric antigen: the cell surface expression of lysozyme-specific Ig V-domains fused to truncated Epo receptor.
Journal of immunological methods 2000;241(1-2):159-70.
2000: S Takemura; Ryutaro Asano; Kouhei Tsumoto; T Arai; N Sakurai; H Kodama; H Yoshida; Yu Katayose; Masanori Suzuki; S Matsuno; Toshio Kudo; Izumi Kumagai
Functional Fv fragment of an antibody specific for CD28: Fv-mediated co-stimulation of T cells.
FEBS letters 2000;476(3):266-71.
2000: Ryoichi Arai; Hiroki R Ueda; Kouhei Tsumoto; Walt Mahoney; Izumi Kumagai; Teruyuki Nagamune
Fluorolabeling of antibody variable domains with green fluorescent protein variants: application to an energy transfer-based homogeneous immunoassay.
Protein engineering 2000;13(5):369-76.
2000: Yoshiyuki Nishimiya; Kouhei Tsumoto; Mitsunori Shiroishi; K Yutani; Izumi Kumagai
Thermodynamic consequences of grafting enhanced affinity toward the mutated antigen onto an antibody. The case of anti-lysozyme antibody, HyHEL-10.
The Journal of biological chemistry 2000;275(17):12813-20.
2000: Ryutaro Asano; S Takemura; Kouhei Tsumoto; N Sakurai; A Teramae; S Ebara; Yu Katayose; M Shinoda; Masanori Suzuki; K Imai; S Matsuno; Toshio Kudo; Izumi Kumagai
Functional construction of the anti-mucin core protein (MUC1) antibody MUSE11 variable regions in a bacterial expression system.
Journal of biochemistry 2000;127(4):673-9.
1999: Hiroki R Ueda; K Kubota; Y Wang; Kouhei Tsumoto; Walt Mahoney; Izumi Kumagai; Teruyuki Nagamune
Homogeneous noncompetitive immunoassay based on the energy transfer between fluorolabeled antibody variable domains (open sandwich fluoroimmunoassay).
BioTechniques 1999;27(4):738-42.
1999: Hidemasa Kondo; Mitsunori Shiroishi; M Matsushima; Kouhei Tsumoto; Izumi Kumagai
Crystal structure of anti-Hen egg white lysozyme antibody (HyHEL-10) Fv-antigen complex. Local structural changes in the protein antigen and water-mediated interactions of Fv-antigen and light chain-heavy chain interfaces.
The Journal of biological chemistry 1999;274(39):27623-31.
1999: C Suzuki; Hiroki R Ueda; Kouhei Tsumoto; Walt Mahoney; Izumi Kumagai; Teruyuki Nagamune
Open sandwich ELISA with V(H)-/V(L)-alkaline phosphatase fusion proteins.
Journal of immunological methods 1999;224(1-2):171-84.
1999: T K Chaudhuri; Kazuyoshi Horii; Takao Yoda; Munehito Arai; S Nagata; Takaho Terada; H Uchiyama; Teikichi Ikura; Kouhei Tsumoto; H Kataoka; M Matsushima; Kunihiro Kuwajima; Izumi Kumagai
Effect of the extra n-terminal methionine residue on the stability and folding of recombinant alpha-lactalbumin expressed in Escherichia coli.
Journal of molecular biology 1999;285(3):1179-94.
1998: Kouhei Tsumoto; K Shinoki; Hidemasa Kondo; M Uchikawa; T Juji; Izumi Kumagai
Highly efficient recovery of functional single-chain Fv fragments from inclusion bodies overexpressed in Escherichia coli by controlled introduction of oxidizing reagent--application to a human single-chain Fv fragment.
Journal of immunological methods 1998;219(1-2):119-29.
1998: M Furuta; M Uchikawa; Y Ueda; T Yabe; T Taima; Kouhei Tsumoto; S Kojima; T Juji; Izumi Kumagai
Construction of mono- and bivalent human single-chain Fv fragments against the D antigen in the Rh blood group: multimerization effect on cell agglutination and application to blood typing.
Protein engineering 1998;11(3):233-41.
1997: Kouhei Tsumoto; Yoshiyuki Nishimiya; N Kasai; Hiroshi Ueda; T Nagamune; K Ogasahara; K Yutani; K Tokuhisa; M Matsushima; Izumi Kumagai
Novel selection method for engineered antibodies using the mechanism of Fv fragment stabilization in the presence of antigen.
Protein engineering 1997;10(11):1311-8.
1996: Kouhei Tsumoto; K Ogasahara; Y Ueda; K Watanabe; K Yutani; Izumi Kumagai
Role of salt bridge formation in antigen-antibody interaction. Entropic contribution to the complex between hen egg white lysozyme and its monoclonal antibody HyHEL10.
The Journal of biological chemistry 1996;271(51):32612-6.
1996: Hiroki R Ueda; Kouhei Tsumoto; K Kubota; E Suzuki; Teruyuki Nagamune; H Nishimura; P A Schueler; G Winter; Izumi Kumagai; W C Mohoney
Open sandwich ELISA: a novel immunoassay based on the interchain interaction of antibody variable region.
Nature biotechnology 1996;14(13):1714-8.
1996: K Maenaka; M Furuta; Kouhei Tsumoto; K Watanabe; Y Ueda; Izumi Kumagai
A stable phage-display system using a phagemid vector: phage display of hen egg-white lysozyme (HEL), Escherichia coli alkaline, phosphatase, and anti-HEL monoclonal antibody, HyHEL10.
Biochemical and biophysical research communications 1996;218(3):682-7.
1995: Kouhei Tsumoto; K Ogasahara; Y Ueda; K Watanabe; K Yutani; Izumi Kumagai
Role of Tyr residues in the contact region of anti-lysozyme monoclonal antibody HyHEL10 for antigen binding.
The Journal of biological chemistry 1995;270(31):18551-7.
1994: Kouhei Tsumoto; Y Ueda; K Maenaka; K Watanabe; K Ogasahara; K Yutani; Izumi Kumagai
Contribution to antibody-antigen interaction of structurally perturbed antigenic residues upon antibody binding.
The Journal of biological chemistry 1994;269(46):28777-82.
1994: Kouhei Tsumoto; Y Nakaoki; Y Ueda; K Ogasahara; K Yutani; K Watanabe; Izumi Kumagai
Effect of the order of antibody variable regions on the expression of the single-chain HyHEL10 Fv fragment in E. coli and the thermodynamic analysis of its antigen-binding properties.
Biochemical and biophysical research communications 1994;201(2):546-51.
1993: Y Ueda; Kouhei Tsumoto; K Watanabe; Izumi Kumagai
Synthesis and expression of a DNA encoding the Fv domain of an anti-lysozyme monoclonal antibody, HyHEL10, in Streptomyces lividans.
Gene 1993;129(1):129-34.
1992: Y Ueda; S Kojima; Kouhei Tsumoto; S Takeda; K i Miura; Izumi Kumagai
A protease inhibitor produced by Streptomyces lividans 66 exhibits inhibitory activities toward both subtilisin BPN' and trypsin.
Journal of biochemistry 1992;112(2):204-11.