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Stephen Bottomley

Research Profile

Chemicals & Drugs

Physiology

Protein Folding

Concepts & Ideas

Disorders

Protein Denaturation

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Amino Acid Sequences

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Publications

TOP 3
Anja S Knaupp; Stephen P Bottomley; Weiwen Dai; Shani Keleher; Li Yang; Mary C Pearce
The roles of helix I and strand 5A in the folding, function and misfolding of α1-antitrypsin.
Andre L Samson; Maithili Sashindranath; Helen M Saunders; Cameron J Nowell; Amanda E-L Au; Rachael J Borg; Stephen P Bottomley; Stephen H Cody; Elisa J Cops; Victoria A Hughes; Anja S Knaupp; Catriona A McLean; Robert L Medcalf
Nucleocytoplasmic coagulation: an injury-induced aggregation event that disulfide crosslinks proteins and facilitates their removal by plasmin.
Itamar Kass; Anja S Knaupp; Stephen P Bottomley; Ashley M Buckle
Conformational properties of the disease-causing Z variant of α1-antitrypsin revealed by theory and experiment.

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All Publications

2013: Anja S Knaupp; Stephen P Bottomley; Weiwen Dai; Shani Keleher; Li Yang; Mary C Pearce
The roles of helix I and strand 5A in the folding, function and misfolding of α1-antitrypsin.
PloS one 2013;8(1):e54766.
2012: Andre L Samson; Maithili Sashindranath; Helen M Saunders; Cameron J Nowell; Amanda E-L Au; Rachael J Borg; Stephen P Bottomley; Stephen H Cody; Elisa J Cops; Victoria A Hughes; Anja S Knaupp; Catriona A McLean; Robert L Medcalf
Nucleocytoplasmic coagulation: an injury-induced aggregation event that disulfide crosslinks proteins and facilitates their removal by plasmin.
Cell reports 2012;2(4):889-901.
2012: Itamar Kass; Anja S Knaupp; Stephen P Bottomley; Ashley M Buckle
Conformational properties of the disease-causing Z variant of α1-antitrypsin revealed by theory and experiment.
Biophysical journal 2012;102(12):2856-65.
2012: Amy L Robertson; Stephen P Bottomley
Molecular pathways to polyglutamine aggregation.
Advances in experimental medicine and biology 2012;769():115-24.
2011: Natasha M Ng; James D Pierce; Stephen P Bottomley; Sarah E Boyd; Renee C Duncan; Boris I Ratnikov; Amy L Robertson; Geoffrey I Webb; Lakshmi C Wijeyewickrema; Robert N Pike
Discovery of amino acid motifs for thrombin cleavage and validation using a model substrate.
Biochemistry 2011;50(48):10499-507.
2011: Stephen P Bottomley
The structural diversity in α1-antitrypsin misfolding.
EMBO reports 2011;12(10):983-4.
2011: Helen M Saunders; Yves Dehouck; Dimitri Gilis; Amy L Robertson; Marianne Rooman; Stephen P Bottomley
Flanking domain stability modulates the aggregation kinetics of a polyglutamine disease protein.
Protein science : a publication of the Protein Society 2011;20(10):1675-81.
2011: Anita J Horvath; Bernadine G C Lu; Robert N Pike; Stephen P Bottomley
Methods to measure the kinetics of protease inhibition by serpins.
Methods in enzymology 2011;501():223-35.
2011: Petra Van Damme; Vita Levina; Stephen P Bottomley; Corinne Hitchen; Phillip I Bird
Intracellular production of recombinant serpins in yeast.
Methods in enzymology 2011;501():1-12.
2011: Jennifer Phan; Jason Schmidberger; Nasrin Yamout; Stephen P Bottomley; Ashley M Buckle
Refolding your protein with a little help from REFOLD.
Methods in molecular biology (Clifton, N.J.) 2011;752():45-57.
2011: Amy L Robertson; Mark A Bate; Steve G Androulakis; Stephen P Bottomley; Ashley M Buckle
PolyQ: a database describing the sequence and domain context of polyglutamine repeats in proteins.
Nucleic acids research 2011;39(Database issue):D272-6.
2010: Stephen P Bottomley
The folding pathway of alpha1-antitrypsin: avoiding the unavoidable.
Proceedings of the American Thoracic Society 2010;7(6):404-7.
2010: Mary C Pearce; G A Powers; S C Feil; Guido Hansen; Michael W Parker; Stephen P Bottomley
Identification and characterization of a misfolded monomeric serpin formed at physiological temperature.
Journal of molecular biology 2010;403(3):459-67.
2010: James C Whisstock; Gary A Silverman; Phillip I Bird; Stephen P Bottomley; Petra Van Damme; Cliff J Luke; Stephen C Pak; Jean-Marc Reichhart; James A Huntington
Serpins flex their muscle: II. Structural insights into target peptidase recognition, polymerization, and transport functions.
The Journal of biological chemistry 2010;285(32):24307-12.
2010: Amy L Robertson; Stephen J Headey; Helen M Saunders; Heath Ecroyd; Martin Scanlon; John A Carver; Stephen P Bottomley
Small heat-shock proteins interact with a flanking domain to suppress polyglutamine aggregation.
Proceedings of the National Academy of Sciences of the United States of America 2010;107(23):10424-9.
2010: Anja S Knaupp; Vita Levina; Amy L Robertson; Mary C Pearce; Stephen P Bottomley
Kinetic instability of the serpin Z alpha1-antitrypsin promotes aggregation.
Journal of molecular biology 2010;396(2):375-83.
2010: Ruth M Kennan; Wilson Wong; Om P Dhungyel; Xiaoyan Han; David Wong; Dane Parker; Carlos J Rosado; Ruby H P Law; Sheena McGowan; Shane Reeve; Vita Levina; Glenn A Powers; Robert N Pike; Stephen P Bottomley; A Ian Smith; Ian Marsh; Richard J Whittington; James C Whisstock; Corrine J Porter; Julian I Rood
The subtilisin-like protease AprV2 is required for virulence and uses a novel disulphide-tethered exosite to bind substrates.
PLoS pathogens 2010;6(11):e1001210.
2010: A L Robertson; Stephen P Bottomley
Towards the treatment of polyglutamine diseases: the modulatory role of protein context.
Current medicinal chemistry 2010;17(27):3058-68.
2009: Vita Levina; Weiwen Dai; Anja S Knaupp; Dion Kaiserman; Mary C Pearce; Lisa D Cabrita; Phillip I Bird; Stephen P Bottomley
Expression, purification and characterization of recombinant Z alpha(1)-antitrypsin--the most common cause of alpha(1)-antitrypsin deficiency.
Protein expression and purification 2009;68(2):226-32.
2009: Helen M Saunders; Stephen P Bottomley
Multi-domain misfolding: understanding the aggregation pathway of polyglutamine proteins.
Protein engineering, design & selection : PEDS 2009;22(8):447-51.
2009: Jonathan C Niclis; Andrew Ellisdon; Alan O Trounson; Mirella Dottori; Stephen P Bottomley; Y Verlinsky; David S Cram
Human embryonic stem cell models of Huntington disease.
Reproductive biomedicine online 2009;19(1):106-13.
2009: Jason J Paxman; Natalie A Borg; James Horne; Philip E Thompson; Yanni Chin; Pooja Sharma; Jamie S Simpson; Jerome Wielens; Susannah Piek; Charlene Kahler; Harry Sakellaris; Mary Pearce; Stephen P Bottomley; Jamie Rossjohn; Martin Scanlon
The structure of the bacterial oxidoreductase enzyme DsbA in complex with a peptide reveals a basis for substrate specificity in the catalytic cycle of DsbA enzymes.
The Journal of biological chemistry 2009;284(26):17835-45.
2009: Travis Beddoe; Zhenjun Chen; Craig S Clements; Lauren K Ely; Simon R Bushell; Julian P Vivian; Lars Kjer-Nielsen; Siew Siew Pang; Michelle A Dunstone; Yu Chih Liu; Whitney A Macdonald; Matthew A Perugini; Matthew C J Wilce; Scott R Burrows; Anthony W Purcell; Tony Tiganis; Stephen P Bottomley; James McCluskey; Jamie Rossjohn
Antigen ligation triggers a conformational change within the constant domain of the alphabeta T cell receptor.
Immunity 2009;30(6):777-88.
2009: Poh Chee Ong; Sarah J Golding; Mary C Pearce; James Irving; Sergei A Grigoryev; Debbie Pike; Christopher G Langendorf; Tanya A Bashtannyk-Puhalovich; Stephen P Bottomley; James C Whisstock; Robert N Pike; Sheena McGowan
Conformational change in the chromatin remodelling protein MENT.
PloS one 2009;4(3):e4727.
2009: Anja S Knaupp; Stephen P Bottomley
Serpin polymerization and its role in disease--the molecular basis of alpha1-antitrypsin deficiency.
IUBMB life 2009;61(1):1-5.
2008: Julian P Vivian; Jessica Scoullar; Amy L Robertson; Stephen P Bottomley; James Horne; Yanni Chin; Jerome Wielens; Philip E Thompson; Tony Velkov; Susannah Piek; Emma Byres; Travis Beddoe; Matthew C J Wilce; Charlene Kahler; Jamie Rossjohn; Martin Scanlon
Structural and biochemical characterization of the oxidoreductase NmDsbA3 from Neisseria meningitidis.
The Journal of biological chemistry 2008;283(47):32452-61.
2008: Zara Fulton; Adrian McAlister; Matthew C J Wilce; Rajini Brammananth; Leyla Zaker-Tabrizi; Matthew A Perugini; Stephen P Bottomley; Ross Coppel; PK Crellin; Jamie Rossjohn; Travis Beddoe
Crystal structure of a UDP-glucose-specific glycosyltransferase from a Mycobacterium species.
The Journal of biological chemistry 2008;283(41):27881-90.
2008: Deborah J Tew; Stephen P Bottomley; David P Smith; Giuseppe D Ciccotosto; jeffrey babon; Mark G Hinds; Colin L Masters; Roberto Cappai; Kevin J Barnham
Stabilization of neurotoxic soluble beta-sheet-rich conformations of the Alzheimer's disease amyloid-beta peptide.
Biophysical journal 2008;94(7):2752-66.
2008: Qingwei Zhang; Ruby H P Law; Stephen P Bottomley; James C Whisstock; Ashley M Buckle
A structural basis for loop C-sheet polymerization in serpins.
Journal of molecular biology 2008;376(5):1348-59.
2008: Steven Harrison; Nicholas G Housden; Stephen P Bottomley; Aimee J Cossins; Michael G Gore
Generation and expression of a minimal hybrid Ig-receptor formed between single domains from proteins L and G.
Protein expression and purification 2008;58(1):12-22.
2008: David P Smith; Deborah J Tew; Andrew Hill; Stephen P Bottomley; Colin L Masters; Kevin J Barnham; Roberto Cappai
Formation of a high affinity lipid-binding intermediate during the early aggregation phase of alpha-synuclein.
Biochemistry 2008;47(5):1425-34.
2007: Poh Chee Ong; Sheena McGowan; Mary C Pearce; James Irving; Wan-Ting Kan; Sergei A Grigoryev; Boris Turk; Gary A Silverman; Klaudia Brix; Stephen P Bottomley; James C Whisstock; Robert N Pike
DNA accelerates the inhibition of human cathepsin V by serpins.
The Journal of biological chemistry 2007;282(51):36980-6.
2007: Lisa D Cabrita; Dimitri Gilis; Amy L Robertson; Yves Dehouck; Marianne Rooman; Stephen P Bottomley
Enhancing the stability and solubility of TEV protease using in silico design.
Protein science : a publication of the Protein Society 2007;16(11):2360-7.
2007: Carlos J Rosado; Ashley M Buckle; Ruby H P Law; Rebecca E Butcher; Wan-Ting Kan; Catherina H Bird; Kheng Ung; Kylie A Browne; Katherine Baran; Tanya A Bashtannyk-Puhalovich; Noel G Faux; Wilson Wong; Corrine J Porter; Robert N Pike; Andrew Ellisdon; Mary C Pearce; Stephen P Bottomley; jonas emsley; A Ian Smith; Jamie Rossjohn; Elizabeth L Hartland; Ilia Voskoboinik; Joseph A Trapani; Phillip I Bird; Michelle A Dunstone; James C Whisstock
A common fold mediates vertebrate defense and bacterial attack.
Science (New York, N.Y.) 2007;317(5844):1548-51.
2007: Lisa D Cabrita; James Irving; Mary C Pearce; James C Whisstock; Stephen P Bottomley
Aeropin from the extremophile Pyrobaculum aerophilum bypasses the serpin misfolding trap.
The Journal of biological chemistry 2007;282(37):26802-9.
2007: Mary C Pearce; Lisa D Cabrita; Andrew Ellisdon; Stephen P Bottomley
The loss of tryptophan 194 in antichymotrypsin lowers the kinetic barrier to misfolding.
The FEBS journal 2007;274(14):3622-32.
2007: Qingwei Zhang; Ashley M Buckle; Ruby H P Law; Mary C Pearce; Lisa D Cabrita; Gordon J Lloyd; James Irving; A Ian Smith; Katya Ruzyla; Jamie Rossjohn; Stephen P Bottomley; James C Whisstock
The N terminus of the serpin, tengpin, functions to trap the metastable native state.
EMBO reports 2007;8(7):658-63.
2007: Andrew Ellisdon; Mary C Pearce; Stephen P Bottomley
Mechanisms of ataxin-3 misfolding and fibril formation: kinetic analysis of a disease-associated polyglutamine protein.
Journal of molecular biology 2007;368(2):595-605.
2007: Glenn A Powers; Chi L L Pham; Mary C Pearce; Geoffrey J Howlett; Stephen P Bottomley
Serpin acceleration of amyloid fibril formation: a role for accessory proteins.
Journal of molecular biology 2007;366(2):666-76.
2006: James C Whisstock; Stephen P Bottomley
Molecular gymnastics: serpin structure, folding and misfolding.
Current opinion in structural biology 2006;16(6):761-8.
2006: Simon R Bushell; Stephen P Bottomley; Jamie Rossjohn; Travis Beddoe
Tracking the unfolding pathway of a multirepeat protein via tryptophan scanning: evidence of localized instability in the mitochondrial import receptor Tom70.
The Journal of biological chemistry 2006;281(34):24345-50.
2006: Sheena McGowan; Ashley M Buckle; James Irving; Poh Chee Ong; Tanya A Bashtannyk-Puhalovich; Wan-Ting Kan; Kate N Henderson; Yaroslava A Bulynko; Evgenya Y Popova; A Ian Smith; Stephen P Bottomley; Jamie Rossjohn; Sergei A Grigoryev; Robert N Pike; James C Whisstock
X-ray crystal structure of MENT: evidence for functional loop-sheet polymers in chromatin condensation.
The EMBO journal 2006;25(13):3144-55.
2006: Andrew Ellisdon; Bronwen Thomas; Stephen P Bottomley
The two-stage pathway of ataxin-3 fibrillogenesis involves a polyglutamine-independent step.
The Journal of biological chemistry 2006;281(25):16888-96.
2006: Michelle K M Chow; Abdullah A Amin; Kate F Fulton; James C Whisstock; Ashley M Buckle; Stephen P Bottomley
REFOLD: an analytical database of protein refolding methods.
Protein expression and purification 2006;46(1):166-71.
2006: Michelle K M Chow; Andrew Ellisdon; Lisa D Cabrita; Stephen P Bottomley
Purification of polyglutamine proteins.
Methods in enzymology 2006;413():1-19.
2006: Lisa D Cabrita; Weiwen Dai; Stephen P Bottomley
A family of E. coli expression vectors for laboratory scale and high throughput soluble protein production.
BMC biotechnology 2006;6():12.
2006: Michelle K M Chow; Abdullah A Amin; Kate F Fulton; Thushan Fernando; Lawrence Kamau; Chris Batty; Michael Louca; Storm Ho; James C Whisstock; Stephen P Bottomley; Ashley M Buckle
The REFOLD database: a tool for the optimization of protein expression and refolding.
Nucleic acids research 2006;34(Database issue):D207-12.
2005: Anita J Horvath; James Irving; Jamie Rossjohn; Ruby H P Law; Stephen P Bottomley; Noelene S Quinsey; Robert N Pike; Paul B Coughlin; James C Whisstock
The murine orthologue of human antichymotrypsin: a structural paradigm for clade A3 serpins.
The Journal of biological chemistry 2005;280(52):43168-78.
2005: James C Whisstock; Stephen P Bottomley; Phillip I Bird; Robert N Pike; Paul B Coughlin
Serpins 2005 - fun between the beta-sheets. Meeting report based upon presentations made at the 4th International Symposium on Serpin Structure, Function and Biology (Cairns, Australia).
The FEBS journal 2005;272(19):4868-73.
2005: Roberto Cappai; Su-Ling Leck; Deborah J Tew; Nicholas A Williamson; David P Smith; Denise Galatis; Robyn A Sharples; Cyril C Curtain; Feda E Ali; Robert A Cherny; Janetta G Culvenor; Stephen P Bottomley; Colin L Masters; Kevin J Barnham; Andrew Hill
Dopamine promotes alpha-synuclein aggregation into SDS-resistant soluble oligomers via a distinct folding pathway.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2005;19(10):1377-9.
2005: Ruby H P Law; James Irving; Ashley M Buckle; Katya Ruzyla; Marguerite S Buzza; Tanya A Bashtannyk-Puhalovich; Travis Beddoe; Kim Nguyen; D Margaret Worrall; Stephen P Bottomley; Phillip I Bird; Jamie Rossjohn; James C Whisstock
The high resolution crystal structure of the human tumor suppressor maspin reveals a novel conformational switch in the G-helix.
The Journal of biological chemistry 2005;280(23):22356-64.
2005: Lauren K Ely; Katherine J Green; Travis Beddoe; Craig S Clements; John J Miles; Stephen P Bottomley; Danielle Zernich; Lars Kjer-Nielsen; Anthony W Purcell; James McCluskey; Jamie Rossjohn; Scott R Burrows
Antagonism of antiviral and allogeneic activity of a human public CTL clonotype by a single altered peptide ligand: implications for allograft rejection.
Journal of immunology (Baltimore, Md. : 1950) 2005;174(9):5593-601.
2005: Noel G Faux; Stephen P Bottomley; Arthur M Lesk; James Irving; John R Morrison; Maria Garcia de la Banda; James C Whisstock
Functional insights from the distribution and role of homopeptide repeat-containing proteins.
Genome research 2005;15(4):537-51.
2005: Kate F Fulton; Ashley M Buckle; Lisa D Cabrita; James Irving; Rebecca E Butcher; Ian Smith; Shane Reeve; Arthur M Lesk; Stephen P Bottomley; Jamie Rossjohn; James C Whisstock
The high resolution crystal structure of a native thermostable serpin reveals the complex mechanism underpinning the stressed to relaxed transition.
The Journal of biological chemistry 2005;280(9):8435-42.
2005: Ashley M Buckle; Glyn L Devlin; Rachel A Jodun; Kate F Fulton; Noel G Faux; James C Whisstock; Stephen P Bottomley
The matrix refolded.
Nature methods 2005;2(1):3.
2005: Kate F Fulton; Glyn L Devlin; Rachel A Jodun; Linda Silvestri; Stephen P Bottomley; Alan Fersht; Ashley M Buckle
PFD: a database for the investigation of protein folding kinetics and stability.
Nucleic acids research 2005;33(Database issue):D279-83.
2005: Glyn L Devlin; Stephen P Bottomley
A protein family under 'stress' - serpin stability, folding and misfolding.
Frontiers in bioscience : a journal and virtual library 2005;10():288-99.
2004: Mary C Pearce; Lisa D Cabrita; Harvey Rubin; Michael G Gore; Stephen P Bottomley
Identification of residual structure within denatured antichymotrypsin: implications for serpin folding and misfolding.
Biochemical and biophysical research communications 2004;324(2):729-35.
2004: Michelle K M Chow; Andrew Ellisdon; Lisa D Cabrita; Stephen P Bottomley
Polyglutamine expansion in ataxin-3 does not affect protein stability: implications for misfolding and disease.
The Journal of biological chemistry 2004;279(46):47643-51.
2004: Travis Beddoe; Simon R Bushell; Matthew A Perugini; Trevor Lithgow; Terrence D Mulhern; Stephen P Bottomley; Jamie Rossjohn
A biophysical analysis of the tetratricopeptide repeat-rich mitochondrial import receptor, Tom70, reveals an elongated monomer that is inherently flexible, unstable, and unfolds via a multistate pathway.
The Journal of biological chemistry 2004;279(45):46448-54.
2004: Michelle K M Chow; Joel Mackay; James C Whisstock; Martin Scanlon; Stephen P Bottomley
Structural and functional analysis of the Josephin domain of the polyglutamine protein ataxin-3.
Biochemical and biophysical research communications 2004;322(2):387-94.
2004: Lisa D Cabrita; Weiwen Dai; Stephen P Bottomley
Different conformational changes within the F-helix occur during serpin folding, polymerization, and proteinase inhibition.
Biochemistry 2004;43(30):9834-9.
2004: Danielle Zernich; Anthony W Purcell; Whitney A Macdonald; Lars Kjer-Nielsen; Lauren K Ely; Nihay Laham; Tanya Crockford; Nicole Mifsud; Mandvi Bharadwaj; Linus Chang; Brian D Tait; Rhonda Holdsworth; Andrew G Brooks; Stephen P Bottomley; Travis Beddoe; Chen Au Peh; Jamie Rossjohn; James McCluskey
Natural HLA class I polymorphism controls the pathway of antigen presentation and susceptibility to viral evasion.
The Journal of experimental medicine 2004;200(1):13-24.
2004: Lauren N Benning; James C Whisstock; Jiuru Sun; Phillip I Bird; Stephen P Bottomley
The human serpin proteinase inhibitor-9 self-associates at physiological temperatures.
Protein science : a publication of the Protein Society 2004;13(7):1859-64.
2004: Andrew Webb; Natalie A Borg; Michelle A Dunstone; Lars Kjer-Nielsen; Travis Beddoe; James McCluskey; Francis R Carbone; Stephen P Bottomley; Marie-Isabel Aguilar; Anthony W Purcell; Jamie Rossjohn
The structure of H-2K(b) and K(bm8) complexed to a herpes simplex virus determinant: evidence for a conformational switch that governs T cell repertoire selection and viral resistance.
Journal of immunology (Baltimore, Md. : 1950) 2004;173(1):402-9.
2004: Lisa D Cabrita; Stephen P Bottomley
How do proteins avoid becoming too stable? Biophysical studies into metastable proteins.
European biophysics journal : EBJ 2004;33(2):83-8.
2004: Nicholas G Housden; Steven Harrison; Hazel R Housden; Karen-Anne Thomas; Jennifer A Beckingham; Siân E Roberts; Stephen P Bottomley; Marc Graille; Enrico A Stura; Michael G Gore
Observation and characterization of the interaction between a single immunoglobulin binding domain of protein L and two equivalents of human kappa light chains.
The Journal of biological chemistry 2004;279(10):9370-8.
2004: Andrew Ellisdon; Stephen P Bottomley
The role of protein misfolding in the pathogenesis of human diseases.
IUBMB life 2004;56(3):119-23.
2004: Noelene S Quinsey; Ainslie L Greedy; Stephen P Bottomley; James C Whisstock; Robert N Pike
Antithrombin: in control of coagulation.
The international journal of biochemistry & cell biology 2004;36(3):386-9.
2004: Michelle K M Chow; David A Lomas; Stephen P Bottomley
Promiscuous beta-strand interactions and the conformational diseases.
Current medicinal chemistry 2004;11(4):491-9.
2004: Phillip I Bird; Stephen C Pak; D Margaret Worrall; Stephen P Bottomley
Production of recombinant serpins in Escherichia coli.
Methods (San Diego, Calif.) 2004;32(2):169-76.
2004: Michelle K M Chow; Henry L Paulson; Stephen P Bottomley
Destabilization of a non-pathological variant of ataxin-3 results in fibrillogenesis via a partially folded intermediate: a model for misfolding in polyglutamine disease.
Journal of molecular biology 2004;335(1):333-41.
2004: Lisa D Cabrita; Stephen P Bottomley
Protein expression and refolding--a practical guide to getting the most out of inclusion bodies.
Biotechnology annual review 2004;10():31-50.
2003: Glyn L Devlin; John A Carver; Stephen P Bottomley
The selective inhibition of serpin aggregation by the molecular chaperone, alpha-crystallin, indicates a nucleation-dependent specificity.
The Journal of biological chemistry 2003;278(49):48644-50.
2003: Noelene S Quinsey; Hazel L Fitton; Paul B Coughlin; James C Whisstock; Timothy R Dafforn; Robin W Carrell; Stephen P Bottomley; Robert N Pike
Introduction of a mutation in the shutter region of antithrombin (Phe77 --> Leu) increases affinity for heparin and decreases thermal stability.
Biochemistry 2003;42(34):10169-73.
2003: James Irving; Lisa D Cabrita; Jamie Rossjohn; Robert N Pike; Stephen P Bottomley; James C Whisstock
The 1.5 A crystal structure of a prokaryote serpin: controlling conformational change in a heated environment.
Structure (London, England : 1993) 2003;11(4):387-97.
2003: Justin P Ludeman; Robert N Pike; Karen M Bromfield; Peter J Duggan; Julia Cianci; Bernard F Le Bonniec; James C Whisstock; Stephen P Bottomley
Determination of the P1', P2' and P3' subsite-specificity of factor Xa.
The international journal of biochemistry & cell biology 2003;35(2):221-5.
2003: Dimitri Gilis; Holly R McLennan; Yves Dehouck; Lisa D Cabrita; Marianne Rooman; Stephen P Bottomley
In vitro and in silico design of alpha1-antitrypsin mutants with different conformational stabilities.
Journal of molecular biology 2003;325(3):581-9.
2002: Glyn L Devlin; Michelle K M Chow; Geoffrey J Howlett; Stephen P Bottomley
Acid Denaturation of alpha1-antitrypsin: characterization of a novel mechanism of serpin polymerization.
Journal of molecular biology 2002;324(4):859-70.
2002: Robert N Pike; Stephen P Bottomley; James Irving; Phillip I Bird; James C Whisstock
Serpins: finely balanced conformational traps.
IUBMB life 2002;54(1):1-7.
2002: Noelene S Quinsey; James C Whisstock; Bernard F Le Bonniec; Virginie Louvain; Stephen P Bottomley; Robert N Pike
Molecular determinants of the mechanism underlying acceleration of the interaction between antithrombin and factor Xa by heparin pentasaccharide.
The Journal of biological chemistry 2002;277(18):15971-8.
2002: James Irving; Robert N Pike; Weiwen Dai; Dieter Brömme; D Margaret Worrall; Gary A Silverman; Theresa H T Coetzer; Clive Dennison; Stephen P Bottomley; James C Whisstock
Evidence that serpin architecture intrinsically supports papain-like cysteine protease inhibition: engineering alpha(1)-antitrypsin to inhibit cathepsin proteases.
Biochemistry 2002;41(15):4998-5004.
2002: James Irving; Sain S Shushanov; Robert N Pike; Evgenya Y Popova; Dieter Brömme; Theresa H T Coetzer; Stephen P Bottomley; Iaroslava A Boulynko; Sergei A Grigoryev; James C Whisstock
Inhibitory activity of a heterochromatin-associated serpin (MENT) against papain-like cysteine proteinases affects chromatin structure and blocks cell proliferation.
The Journal of biological chemistry 2002;277(15):13192-201.
2002: Lisa D Cabrita; James C Whisstock; Stephen P Bottomley
Probing the role of the F-helix in serpin stability through a single tryptophan substitution.
Biochemistry 2002;41(14):4575-81.
2002: Michael R de Niese; Carla Chinni; Robert N Pike; Stephen P Bottomley; Eleanor J Mackie
Dissection of protease-activated receptor-1-dependent and -independent responses to thrombin in skeletal myoblasts.
Experimental cell research 2002;274(1):149-56.
2001: Stephen P Bottomley; I D Lawrenson; DJ Tew; Weiwen Dai; James C Whisstock; Robert N Pike
The role of strand 1 of the C beta-sheet in the structure and function of alpha(1)-antitrypsin.
Protein science : a publication of the Protein Society 2001;10(12):2518-24.
2001: DJ Tew; Stephen P Bottomley
Probing the equilibrium denaturation of the serpin alpha(1)-antitrypsin with single tryptophan mutants; evidence for structure in the urea unfolded state.
Journal of molecular biology 2001;313(5):1161-9.
2001: Michelle K M Chow; Glyn L Devlin; Stephen P Bottomley
Osmolytes as modulators of conformational changes in serpins.
Biological chemistry 2001;382(11):1593-9.
2001: Marc Graille; Enrico A Stura; Nicholas G Housden; Jennifer A Beckingham; Stephen P Bottomley; Dennis Beale; Michael J Taussig; Brian J Sutton; Michael G Gore; Jean-Baptiste Charbonnier
Complex between Peptostreptococcus magnus protein L and a human antibody reveals structural convergence in the interaction modes of Fab binding proteins.
Structure (London, England : 1993) 2001;9(8):679-87.
2001: Glyn L Devlin; Helen Parfrey; DJ Tew; David A Lomas; Stephen P Bottomley
Prevention of polymerization of M and Z alpha1-Antitrypsin (alpha1-AT) with trimethylamine N-oxide. Implications for the treatment of alpha1-at deficiency.
American journal of respiratory cell and molecular biology 2001;24(6):727-32.
2001: DJ Tew; Stephen P Bottomley
Intrinsic fluorescence changes and rapid kinetics of proteinase deformation during serpin inhibition.
FEBS letters 2001;494(1-2):30-3.
2001: Jennifer A Beckingham; Nicholas G Housden; NM Muir; Stephen P Bottomley; Michael G Gore
Studies on a single immunoglobulin-binding domain of protein L from Peptostreptococcus magnus: the role of tyrosine-53 in the reaction with human IgG.
The Biochemical journal 2001;353(Pt 2):395-401.
2001: J P Ludeman; James C Whisstock; Paul C R Hopkins; Bernard F Le Bonniec; Stephen P Bottomley
Structure of a serpin-enzyme complex probed by cysteine substitutions and fluorescence spectroscopy.
Biophysical journal 2001;80(1):491-7.
2000: Carla Chinni; Michael R de Niese; AL Jenkins; Robert N Pike; Stephen P Bottomley; Eleanor J Mackie
Protease-activated receptor-2 mediates proliferative responses in skeletal myoblasts.
Journal of cell science 2000;113 Pt 24():4427-33.
2000: James C Whisstock; S Romero; Rajendra Gurung; Harshal H Nandurkar; Lisa M Ooms; Stephen P Bottomley; Christina A Mitchell
The inositol polyphosphate 5-phosphatases and the apurinic/apyrimidinic base excision repair endonucleases share a common mechanism for catalysis.
The Journal of biological chemistry 2000;275(47):37055-61.
2000: Mary C Pearce; H Rubin; Stephen P Bottomley
Conformational change and intermediates in the unfolding of alpha 1-antichymotrypsin.
The Journal of biological chemistry 2000;275(37):28513-8.
2000: Stephen P Bottomley; DJ Tew
The citrate ion increases the conformational stability of alpha(1)-antitrypsin.
Biochimica et biophysica acta 2000;1481(1):11-7.
2000: Michelle A Dunstone; W Dai; James C Whisstock; Jamie Rossjohn; Robert N Pike; Susanne C Feil; Bernard F Le Bonniec; Michael W Parker; Stephen P Bottomley
Cleaved antitrypsin polymers at atomic resolution.
Protein science : a publication of the Protein Society 2000;9(2):417-20.
1999: James C Whisstock; James Irving; Stephen P Bottomley; Robert N Pike; Arthur M Lesk
Serpins in the Caenorhabditis elegans genome.
Proteins 1999;36(1):31-41.
1999: Jennifer A Beckingham; Stephen P Bottomley; R Hinton; Brian J Sutton; Michael G Gore
Interactions between a single immunoglobulin-binding domain of protein L from Peptostreptococcus magnus and a human kappa light chain.
The Biochemical journal 1999;340 ( Pt 1)():193-9.
1999: E L James; James C Whisstock; Michael G Gore; Stephen P Bottomley
Probing the unfolding pathway of alpha1-antitrypsin.
The Journal of biological chemistry 1999;274(14):9482-8.
1999: Carla Chinni; Michael R de Niese; D J Tew; AL Jenkins; Stephen P Bottomley; Eleanor J Mackie
Thrombin, a survival factor for cultured myoblasts.
The Journal of biological chemistry 1999;274(14):9169-74.
1999: Fiona L Scott; Claire E Hirst; Jiuru Sun; Catherina H Bird; Stephen P Bottomley; Phillip I Bird
The intracellular serpin proteinase inhibitor 6 is expressed in monocytes and granulocytes and is a potent inhibitor of the azurophilic granule protease, cathepsin G.
Blood 1999;93(6):2089-97.
1999: N S Preston; D J Baker; Stephen P Bottomley; Michael G Gore
The production and characterisation of an immobilised chaperonin system.
Biochimica et biophysica acta 1999;1426(1):99-109.
1998: Stephen P Bottomley; SR Stone
Protein engineering of chimeric Serpins: an investigation into effects of the serpin scaffold and reactive centre loop length.
Protein engineering 1998;11(12):1243-7.
1998: Stephen P Bottomley; Paul C R Hopkins; James C Whisstock
Alpha 1-antitrypsin polymerisation can occur by both loop A and C sheet mechanisms.
Biochemical and biophysical research communications 1998;251(1):1-5.
1998: E L James; Stephen P Bottomley
The mechanism of alpha 1-antitrypsin polymerization probed by fluorescence spectroscopy.
Archives of biochemistry and biophysics 1998;356(2):296-300.
1998: N L Brown; Stephen P Bottomley; M D Scawen; Michael G Gore
A study of the interactions between an IgG-binding domain based on the B domain of staphylococcal protein A and rabbit IgG.
Molecular biotechnology 1998;10(1):9-16.
1998: N L Brown; Stephen P Bottomley; Michael G Gore
Affinity purification of human IgG using immobilised, mutated immunoglobulin-binding domains from protein A of Staphylococcus aureus.
Biochemical Society transactions 1998;26(3):S249.
1998: Carla Chinni; Stephen P Bottomley; E J Duffy; B A Hemmings; SR Stone
Expression and purification of the human thrombin receptor.
Protein expression and purification 1998;13(1):9-15.
1997: Stephen P Bottomley; W S Chang
The effects of reactive centre loop length upon serpin polymerisation.
Biochemical and biophysical research communications 1997;241(2):264-9.
1997: Jiuru Sun; Stephen P Bottomley; S Kumar; Phillip I Bird
Recombinant caspase-3 expressed in Pichia pastoris is fully activated and kinetically indistinguishable from the native enzyme.
Biochemical and biophysical research communications 1997;238(3):920-4.
1997: Jennifer A Beckingham; Stephen P Bottomley; R J Hinton; B Sutton; Michael G Gore
Equilibrium and pre-equilibrium fluorescence studies on the interaction between protein L and kappa light chain.
Biochemical Society transactions 1997;25(1):38S.
1997: SR Stone; James C Whisstock; Stephen P Bottomley; Paul C R Hopkins
Serpins. A mechanistic class of their own.
Advances in experimental medicine and biology 1997;425():5-15.
1995: Maninder K Sohi; Tommy Wan; Brian J Sutton; T Atkinson; M A Atkinson; J P Murphy; Stephen P Bottomley; Michael G Gore
Crystallization and X-ray analysis of a single fab binding domain from protein L of Peptostreptococcus magnus.
Proteins 1995;23(4):610-2.
1995: K N Walker; Stephen P Bottomley; A G Popplewell; Brian J Sutton; Michael G Gore
Equilibrium and pre-equilibrium fluorescence spectroscopic studies of the binding of a single-immunoglobulin-binding domain derived from protein G to the Fc fragment from human IgG1.
The Biochemical journal 1995;310 ( Pt 1)():177-84.
1995: Stephen P Bottomley; Brian J Sutton; Michael G Gore
Elution of human IgG from affinity columns containing immobilised variants of protein A.
Journal of immunological methods 1995;182(2):185-92.
1995: Stephen P Bottomley; Jennifer A Beckingham; J P Murphy; M Atkinson; T Atkinson; R J Hinton; Michael G Gore
Cloning, expression and purification of Ppl-1, a kappa-chain binding protein, based upon protein L from Peptostreptococcus magnus.
Bioseparation 1995;5(6):359-67.
1994: Stephen P Bottomley; A G Popplewell; M Scawen; T Wan; Brian J Sutton; Michael G Gore
The stability and unfolding of an IgG binding protein based upon the B domain of protein A from Staphylococcus aureus probed by tryptophan substitution and fluorescence spectroscopy.
Protein engineering 1994;7(12):1463-70.
1992: Michael G Gore; Stephen P Bottomley; A G Popplewell; T Atkinson
The use of tryptophan fluorescence to determine the stability of IgG binding proteins based upon the B domain of protein A from Staphylococcus aureus.
Biochemical Society transactions 1992;20(3):288S.