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Jean-Claude Boulain

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Genes & Molecular Sequences

Amino Acid Sequences

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Cloning, Molecular

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Jie-Xian Dong; Frédéric Ducancel; Hong-Tao Lei; Zhen-Feng Li; Yu-Dong Shen; Yuan-Ming Sun; Zhen-Lin Xu; Jin-Yi Yang; Jean-Claude Boulain; Hong Wang
Development of a single-chain variable fragment-alkaline phosphatase fusion protein and a sensitive direct competitive chemiluminescent enzyme immunoassay for detection of ractopamine in pork.
C Magis; D Gasparini; A Lecoq; Marie Hélène Le Du; Enrico A Stura; Jean-Baptiste Charbonnier; Gilles Mourier; Jean-Claude Boulain; L Pardo; A Caruana; A Joly; M Lefranc; Michel Masella; André Menez; Philippe Cuniasse
Structure-based secondary structure-independent approach to design protein ligands: Application to the design of Kv1.2 potassium channel blockers.
Sandrine Braud; Mireille Moutiez; Pascal Belin; Nicolas Abello; Pascal Drevet; Sophie Zinn-Justin; Marie Courçon; Cédric Masson; Janie Dassa; Jean-Baptiste Charbonnier; Jean-Claude Boulain; André Ménez; Roger Genet; Muriel Gondry
Dual expression system suitable for high-throughput fluorescence-based screening and production of soluble proteins.

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All Publications

2012: Jie-Xian Dong; Frédéric Ducancel; Hong-Tao Lei; Zhen-Feng Li; Yu-Dong Shen; Yuan-Ming Sun; Zhen-Lin Xu; Jin-Yi Yang; Jean-Claude Boulain; Hong Wang
Development of a single-chain variable fragment-alkaline phosphatase fusion protein and a sensitive direct competitive chemiluminescent enzyme immunoassay for detection of ractopamine in pork.
Analytica chimica acta 2012;736():85-91.
2006: C Magis; D Gasparini; A Lecoq; Marie Hélène Le Du; Enrico A Stura; Jean-Baptiste Charbonnier; Gilles Mourier; Jean-Claude Boulain; L Pardo; A Caruana; A Joly; M Lefranc; Michel Masella; André Menez; Philippe Cuniasse
Structure-based secondary structure-independent approach to design protein ligands: Application to the design of Kv1.2 potassium channel blockers.
Journal of the American Chemical Society 2006;128(50):16190-205.
2005: Sandrine Braud; Mireille Moutiez; Pascal Belin; Nicolas Abello; Pascal Drevet; Sophie Zinn-Justin; Marie Courçon; Cédric Masson; Janie Dassa; Jean-Baptiste Charbonnier; Jean-Claude Boulain; André Ménez; Roger Genet; Muriel Gondry
Dual expression system suitable for high-throughput fluorescence-based screening and production of soluble proteins.
Journal of proteome research 2005;4(6):2137-47.
2004: Sandrine Braud; Pascal Belin; Janie Dassa; Liliana Pardo; Gilles Mourier; Antony Caruana; Birgit T Priest; Paula M Dulski; Maria L Garcia; André Ménez; Jean-Claude Boulain; Sylvaine Gasparini
BgK, a disulfide-containing sea anemone toxin blocking K+ channels, can be produced in Escherichia coli cytoplasm as a functional tagged protein.
Protein expression and purification 2004;38(1):69-78.
2004: Pascal Belin; Janie Dassa; Pascal Drevet; Evelyne Lajeunesse; Alexandra Savatier; Jean-Claude Boulain; André Ménez
Toxicity-based selection of Escherichia coli mutants for functional recombinant protein production: application to an antibody fragment.
Protein engineering, design & selection : PEDS 2004;17(5):491-500.
2004: Jean-Claude Boulain; Frédéric Ducancel
Expression of recombinant alkaline phosphatase conjugates in Escherichia coli.
Methods in molecular biology (Clifton, N.J.) 2004;267():101-12.
2002: Marie Hélène Le Du; C Lamoure; Bruno H Muller; O V Bulgakov; Evelyne Lajeunesse; André Ménez; Jean-Claude Boulain
Artificial evolution of an enzyme active site: structural studies of three highly active mutants of Escherichia coli alkaline phosphatase.
Journal of molecular biology 2002;316(4):941-53.
2001: Bruno H Muller; C Lamoure; Marie Hélène Le Du; L Cattolico; Evelyne Lajeunesse; F Lemaître; A Pearson; Frédéric Ducancel; André Ménez; Jean-Claude Boulain
Improving Escherichia coli alkaline phosphatase efficacy by additional mutations inside and outside the catalytic pocket.
Chembiochem : a European journal of chemical biology 2001;2(7-8):517-23.
2000: N Germain; K Mérienne; Sophie Zinn-Justin; Jean-Claude Boulain; Frédéric Ducancel; André Ménez
Molecular and structural basis of the specificity of a neutralizing acetylcholine receptor-mimicking antibody, using combined mutational and molecular modeling analyses.
The Journal of biological chemistry 2000;275(28):21578-86.
2000: A Ricciardi; Marie Hélène Le Du; M Khayati; F Dajas; Jean-Claude Boulain; André Menez; Frédéric Ducancel
Do structural deviations between toxins adopting the same fold reflect functional differences?
The Journal of biological chemistry 2000;275(24):18302-10.
2000: Marie Hélène Le Du; A Ricciardi; M Khayati; Renée Ménez; Jean-Claude Boulain; André Ménez; Frédéric Ducancel
Stability of a structural scaffold upon activity transfer: X-ray structure of a three fingers chimeric protein.
Journal of molecular biology 2000;296(4):1017-26.
2000: Yoshiro Chuman; Ikuo Nobuhisa; Tomohisa Ogawa; Masanobu Deshimaru; Takahito Chijiwa; N H Tan; Yasuyuki Fukumaki; Yasuyuki Shimohigashi; Frédéric Ducancel; Jean-Claude Boulain; André Ménez; Motonori Ohno
Regional and accelerated molecular evolution in group I snake venom gland phospholipase A2 isozymes.
Toxicon : official journal of the International Society on Toxinology 2000;38(3):449-62.
1999: Bruno H Muller; D Chevrier; Jean-Claude Boulain; Jean-Luc Guesdon
Recombinant single-chain Fv antibody fragment-alkaline phosphatase conjugate for one-step immunodetection in molecular hybridization.
Journal of immunological methods 1999;227(1-2):177-85.
1998: Michel Léonetti; Robert Thai; J Cotton; S Leroy; Pascal Drevet; Frédéric Ducancel; Jean-Claude Boulain; André Ménez
Increasing immunogenicity of antigens fused to Ig-binding proteins by cell surface targeting.
Journal of immunology (Baltimore, Md. : 1950) 1998;160(8):3820-7.
1998: Motonori Ohno; Renée Ménez; Tomohisa Ogawa; J M Danse; Yasuyuki Shimohigashi; C Fromen; Frédéric Ducancel; Sophie Zinn-Justin; Marie Hélène Le Du; Jean-Claude Boulain; T Tamiya; André Ménez
Molecular evolution of snake toxins: is the functional diversity of snake toxins associated with a mechanism of accelerated evolution?
Progress in nucleic acid research and molecular biology 1998;59():307-64.
1997: C Fromen-Romano; Bernard Maillère; Pascal Drevet; Evelyne Lajeunesse; Frédéric Ducancel; Jean-Claude Boulain; André Ménez
Transformation of a non-enzymatic toxin into a toxoid by genetic engineering.
Protein engineering 1997;10(10):1213-20.
1997: K Mérienne; N Germain; Sophie Zinn-Justin; Jean-Claude Boulain; Frédéric Ducancel; André Ménez
The functional architecture of an acetylcholine receptor-mimicking antibody.
The Journal of biological chemistry 1997;272(38):23775-83.
1997: Pascal Drevet; C Lemaire; Sylvaine Gasparini; Sophie Zinn-Justin; Evelyne Lajeunesse; Frédéric Ducancel; S Pinkasfeld; Marie Courçon; O Tremeau; Jean-Claude Boulain; André Ménez
High-level production and isotope labeling of snake neurotoxins, disulfide-rich proteins.
Protein expression and purification 1997;10(3):293-300.
1997: P Garnier; Frédéric Ducancel; T Ogawa; Jean-Claude Boulain; Françoise Goudey-Perrière; Claude Perrière; A Ménez
Complete amino-acid sequence of the beta-subunit of VTX from venom of the stonefish (Synanceia verrucosa) as identified from cDNA cloning experiments.
Biochimica et biophysica acta 1997;1337(1):1-5.
1996: Frédéric Ducancel; K Mérienne; C Fromen-Romano; O Trémeau; L Pillet; Pascal Drevet; Sophie Zinn-Justin; Jean-Claude Boulain; André Ménez
Mimicry between receptors and antibodies. Identification of snake toxin determinants recognized by the acetylcholine receptor and an acetylcholine receptor-mimicking monoclonal antibody.
The Journal of biological chemistry 1996;271(49):31345-53.
1996: C Chanussot; Laurent Bellanger; C Ligny-Lemaire; P Seguin; André Ménez; Jean-Claude Boulain
Engineering of a recombinant colorimetric fusion protein for immunodiagnosis of insulin.
Journal of immunological methods 1996;197(1-2):39-49.
1996: B Bouhaouala-Zahar; Frédéric Ducancel; I Zenouaki; R Ben Khalifa; Lamia Borchani; Marcel Pelhate; Jean-Claude Boulain; Mohamed El Ayeb; A Ménez; Habib Karoui
A recombinant insect-specific alpha-toxin of Buthus occitanus tunetanus scorpion confers protection against homologous mammal toxins.
European journal of biochemistry / FEBS 1996;238(3):653-60.
1995: A Carrier; Frédéric Ducancel; N B Settiawan; L Cattolico; Bernard Maillère; Michel Léonetti; Pascal Drevet; André Ménez; Jean-Claude Boulain
Recombinant antibody-alkaline phosphatase conjugates for diagnosis of human IgGs: application to anti-HBsAg detection.
Journal of immunological methods 1995;181(2):177-86.
1995: O Trémeau; C Lemaire; Pascal Drevet; S Pinkasfeld; Frédéric Ducancel; Jean-Claude Boulain; André Ménez
Genetic engineering of snake toxins. The functional site of Erabutoxin a, as delineated by site-directed mutagenesis, includes variant residues.
The Journal of biological chemistry 1995;270(16):9362-9.
1994: Sylvaine Gasparini; N Kiyatkin; Pascal Drevet; Jean-Claude Boulain; F Tacnet; Pierre Ripoche; Eric Forest; E Grishin; André Ménez
The low molecular weight protein which co-purifies with alpha-latrotoxin is structurally related to crustacean hyperglycemic hormones.
The Journal of biological chemistry 1994;269(31):19803-9.
1994: Mohamed Amiche; Frédéric Ducancel; A Mor; Jean-Claude Boulain; A Menez; Pierre Nicolas
Precursors of vertebrate peptide antibiotics dermaseptin b and adenoregulin have extensive sequence identities with precursors of opioid peptides dermorphin, dermenkephalin, and deltorphins.
The Journal of biological chemistry 1994;269(27):17847-52.
1994: Sophie Zinn-Justin; L Pillet; Frédéric Ducancel; A Thomas; J C Smith; Jean-Claude Boulain; André Ménez
Engineering of protein epitopes: a single deletion in a snake toxin generates full binding capacity to a previously unrecognized antibody.
Protein engineering 1994;7(7):917-23.
1994: B Arnoux; R Ménez; Pascal Drevet; Jean-Claude Boulain; Arnaud Ducruix; André Ménez
Three-dimensional crystal structure of recombinant erabutoxin a at 2.0 A resolution.
FEBS letters 1994;342(1):12-4.
1994: Eric Ezan; Frédéric Ducancel; Daniel Gillet; Pascal Drevet; André Ménez; JM Grognet; Jean-Claude Boulain
Recombinant technology in the preparation of immunogen and enzymatic tracer. Application to the development of an enzyme immunoassay for rat prolactin.
Journal of immunological methods 1994;169(2):205-11.
1993: Daniel Gillet; Eric Ezan; Frédéric Ducancel; C Gaillard; T Ardouin; M Istin; André Ménez; Jean-Claude Boulain; JM Grognet
Enzyme immunoassay using a rat prolactin-alkaline phosphatase recombinant tracer.
Analytical chemistry 1993;65(13):1779-84.
1993: Frédéric Ducancel; Daniel Gillet; A Carrier; Evelyne Lajeunesse; André Ménez; Jean-Claude Boulain
Recombinant colorimetric antibodies: construction and characterization of a bifunctional F(ab)2/alkaline phosphatase conjugate produced in Escherichia coli.
Bio/technology (Nature Publishing Company) 1993;11(5):601-5.
1993: Mohamed Amiche; Frédéric Ducancel; Evelyne Lajeunesse; Jean-Claude Boulain; A Ménez; Pierre Nicolas
Molecular cloning of a cDNA encoding the precursor of adenoregulin from frog skin. Relationships with the vertebrate defensive peptides, dermaseptins.
Biochemical and biophysical research communications 1993;191(3):983-90.
1993: D Hodgson; Sylvaine Gasparini; Pascal Drevet; Frédéric Ducancel; F Bouet; Jean-Claude Boulain; J B Harris; A Menez
Production of recombinant notechis 11'2L, an enzymatically active mutant of a phospholipase A2 from Notechis scutatus scutatus venom, as directly generated by cleavage of a fusion protein produced in Escherichia coli.
European journal of biochemistry / FEBS 1993;212(2):441-6.
1993: Frédéric Ducancel; V Matre; C Dupont; Evelyne Lajeunesse; Zvi Wollberg; A Bdolah; E Kochva; Jean-Claude Boulain; André Ménez
Cloning and sequence analysis of cDNAs encoding precursors of sarafotoxins. Evidence for an unusual "rosary-type" organization.
The Journal of biological chemistry 1993;268(5):3052-5.
1993: L Pillet; O Trémeau; Frédéric Ducancel; Pascal Drevet; Sophie Zinn-Justin; S Pinkasfeld; Jean-Claude Boulain; André Ménez
Genetic engineering of snake toxins. Role of invariant residues in the structural and functional properties of a curaremimetic toxin, as probed by site-directed mutagenesis.
The Journal of biological chemistry 1993;268(2):909-16.
1992: Daniel Gillet; Frédéric Ducancel; E Pradel; Michel Léonetti; André Ménez; Jean-Claude Boulain
Insertion of a disulfide-containing neurotoxin into E. coli alkaline phosphatase: the hybrid retains both biological activities.
Protein engineering 1992;5(3):273-8.
1992: Frédéric Ducancel; Daniel Gillet; André Ménez; Jean-Claude Boulain
[Recombinant colorimetric antibodies: genetic construction and production in E. coli].
Comptes rendus de l'Académie des sciences. Série III, Sciences de la vie 1992;315(6):221-4.
1991: C Bouchier; P Boyot; F Tesson; O Trémeau; F Bouet; D Hodgson; Jean-Claude Boulain; André Ménez
Notechis 11'2, a non-toxic phospholipase A2 from the venom of Notechis scutatus scutatus.
European journal of biochemistry / FEBS 1991;202(2):493-500.
1991: C Bouchier; Jean-Claude Boulain; C Bon; André Ménez
Analysis of cDNAs encoding the two subunits of crotoxin, a phospholipase A2 neurotoxin from rattlesnake venom: the acidic non enzymatic subunit derives from a phospholipase A2-like precursor.
Biochimica et biophysica acta 1991;1088(3):401-8.
1991: Frédéric Ducancel; Edward G Rowan; E Cassar; A L Harvey; A Ménez; Jean-Claude Boulain
Amino acid sequence of a muscarinic toxin deduced from the cDNA nucleotide sequence.
Toxicon : official journal of the International Society on Toxinology 1991;29(4-5):516-20.
1990: P Boyot; L Pillet; Frédéric Ducancel; Jean-Claude Boulain; O Tremeau; André Ménez
A recombinant snake neurotoxin generated by chemical cleavage of a hybrid protein recovers full biological properties.
FEBS letters 1990;266(1-2):87-90.
1990: Edward G Rowan; Frédéric Ducancel; Y Doljansky; Alan L Harvey; Jean-Claude Boulain; André Ménez
Nucleotide sequence encoding a 'synergistic-like' protein from the venom glands of Dendroaspis angusticeps.
Nucleic acids research 1990;18(6):1639.
1990: Frédéric Ducancel; G Guignery-Frelat; Jean-Claude Boulain; André Ménez
Nucleotide sequence and structure analysis of cDNAs encoding short-chain neurotoxins from venom glands of a sea snake (Aipysurus laevis).
Toxicon : official journal of the International Society on Toxinology 1990;28(1):119-23.
1989: Frédéric Ducancel; Jean-Claude Boulain; O Trémeau; André Ménez
Direct expression in E. coli of a functionally active protein A--snake toxin fusion protein.
Protein engineering 1989;3(2):139-43.
1988: C Bouchier; Frédéric Ducancel; G Guignery-Frelat; C Bon; Jean-Claude Boulain; André Ménez
Cloning and sequencing of cDNAs encoding the two subunits of Crotoxin.
Nucleic acids research 1988;16(18):9050.
1988: Frédéric Ducancel; G Guignery-Frelat; C Bouchier; André Ménez; Jean-Claude Boulain
Complete amino acid sequence of a PLA2 from the tiger snake Notechis scutatus scutatus as deduced from a complementary DNA.
Nucleic acids research 1988;16(18):9049.
1988: Frédéric Ducancel; G Guignery-Frelat; C Bouchier; André Ménez; Jean-Claude Boulain
Sequence analysis of a cDNA encoding a PLA2 from the sea-snake Aipysurus laevis.
Nucleic acids research 1988;16(18):9048.
1987: G Guignery-Frelat; Frédéric Ducancel; André Ménez; Jean-Claude Boulain
Sequence of a cDNA encoding a snake venom phospholipase A2.
Nucleic acids research 1987;15(14):5892.
1986: O Trémeau; Jean-Claude Boulain; J Couderc; P Fromageot; André Ménez
A monoclonal antibody which recognized the functional site of snake neurotoxins and which neutralizes all short-chain variants.
FEBS letters 1986;208(2):236-40.
1985: Jean-Claude Boulain; P Fromageot; André Ménez
Further evidence showing that neurotoxin-acetylcholine receptor dissociation is accelerated by monoclonal neurotoxin-specific immunoglobulin.
Molecular immunology 1985;22(5):553-6.
1984: A Rousselet; G Faure; Jean-Claude Boulain; A Ménez
The interaction of neurotoxin derivatives with either acetylcholine receptor or a monoclonal antibody. An electron-spin-resonance study.
European journal of biochemistry / FEBS 1984;140(1):31-7.
1984: André Ménez; Jean-Claude Boulain; F Bouet; J Couderc; G Faure; A Rousselet; O Trémeau; E Gatineau; P Fromageot
On the molecular mechanisms of neutralization of a cobra neurotoxin by specific antibodies.
Journal de physiologie 1984;79(4):196-206.
1983: G Faure; Jean-Claude Boulain; F Bouet; T Montenay-Garestier; P Fromageot; André Ménez
Role of indole and amino groups in the structure and function of Naja nigricollis toxin alpha.
Biochemistry 1983;22(9):2068-76.
1982: Jean-Claude Boulain; André Ménez; J Couderc; G Faure; P Liacopoulos; P Fromageot
Neutralizing monoclonal antibody specific for Naja nigricollis toxin alpha: preparation, characterization, and localization of the antigenic binding site.
Biochemistry 1982;21(12):2910-5.
1982: A Menez; Jean-Claude Boulain; G Faure; J Couderc; P Liacopoulos; N Tamiya; P Fromageot
Comparison of the "toxic" and antigenic regions in toxin alpha isolated from Naja nigricollis venom.
Toxicon : official journal of the International Society on Toxinology 1982;20(1):95-103.
1981: O Tremeau; G Faure; Jean-Claude Boulain; F Bouet; A Menez; G Lecocq; J L Morgat; P Fromageot
[Binding of a tritiated enkephalinergic analog (FK 33-824) to a mitochondrial fraction of rat brain].
Biochimie 1981;63(6):477-84.