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Claus Urbanke

Research Profile

Concepts & Ideas

Chemicals & Drugs

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Escherichia coli

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Ultracentrifugation

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Amino Acid Sequences

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TOP 3
Renata Z Jurkowska; Wolfgang Nellen; Sergey Ragozin; Arumugam Rajavelu; Claus Urbanke; Nils Anspach; Gytis Jankevicius; Albert Jeltsch
Oligomerization and binding of the Dnmt3a DNA methyltransferase to parallel DNA molecules: heterochromatic localization and role of Dnmt3L.
Arunas Silanskas; Michael Foss; Wolfgang Wende; Claus Urbanke; Arunas Lagunavicius; Alfred Pingoud; Virginijus Siksnys
Photocaged variants of the MunI and PvuII restriction enzymes.
André Schönichen; Janna M Bigalke; Claus Urbanke; Stephan Grzesiek; Sonja A Dames; Matthias Geyer
A flexible bipartite coiled coil structure is required for the interaction of Hexim1 with the P-TEFB subunit cyclin T1.

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All Publications

2011: Renata Z Jurkowska; Wolfgang Nellen; Sergey Ragozin; Arumugam Rajavelu; Claus Urbanke; Nils Anspach; Gytis Jankevicius; Albert Jeltsch
Oligomerization and binding of the Dnmt3a DNA methyltransferase to parallel DNA molecules: heterochromatic localization and role of Dnmt3L.
The Journal of biological chemistry 2011;286(27):24200-7.
2011: Arunas Silanskas; Michael Foss; Wolfgang Wende; Claus Urbanke; Arunas Lagunavicius; Alfred Pingoud; Virginijus Siksnys
Photocaged variants of the MunI and PvuII restriction enzymes.
Biochemistry 2011;50(14):2800-7.
2010: André Schönichen; Janna M Bigalke; Claus Urbanke; Stephan Grzesiek; Sonja A Dames; Matthias Geyer
A flexible bipartite coiled coil structure is required for the interaction of Hexim1 with the P-TEFB subunit cyclin T1.
Biochemistry 2010;49(14):3083-91.
2010: Mamta Amrute-Nayak; Ralph P Diensthuber; Walter Steffen; Daniela Kathmann; Falk K Hartmann; Roman Fedorov; Claus Urbanke; Dietmar J Manstein; Bernhard Brenner; Georgios Tsiavaliaris
Targeted optimization of a protein nanomachine for operation in biohybrid devices.
Angewandte Chemie (International ed. in English) 2010;49(2):312-6.
2008: Renata Z Jurkowska; Nils Anspach; Claus Urbanke; Da Jia; Richard Reinhardt; Wolfgang Nellen; Xiaodong Cheng; Albert Jeltsch
Formation of nucleoprotein filaments by mammalian DNA methyltransferase Dnmt3a in complex with regulator Dnmt3L.
Nucleic acids research 2008;36(21):6656-63.
2008: Dennis Breitsprecher; Antje K Kiesewetter; Joern Linkner; Claus Urbanke; Guenter P Resch; J Victor Small; Jan Faix
Clustering of VASP actively drives processive, WH2 domain-mediated actin filament elongation.
The EMBO journal 2008;27(22):2943-54.
2008: G Arad; Ayal Hendel; Claus Urbanke; Ute Curth; Zvi Livneh
Single-stranded DNA-binding protein recruits DNA polymerase V to primer termini on RecA-coated DNA.
The Journal of biological chemistry 2008;283(13):8274-82.
2008: Giedrius Gasiunas; Giedrius Sasnauskas; Gintautas Tamulaitis; Claus Urbanke; Dalia Razaniene; Virginijus Siksnys
Tetrameric restriction enzymes: expansion to the GIY-YIG nuclease family.
Nucleic acids research 2008;36(3):938-49.
2007: Monika Sokolowska; Magdalena Kaus-Drobek; Honorata Czapinska; Gintautas Tamulaitis; Roman H Szczepanowski; Claus Urbanke; Virginijus Siksnys; Matthias Bochtler
Monomeric restriction endonuclease BcnI in the apo form and in an asymmetric complex with target DNA.
Journal of molecular biology 2007;369(3):722-34.
2007: Mathias Lübben; Jörn Güldenhaupt; Martin Zoltner; Katrin Deigweiher; Peter Haebel; Claus Urbanke; Axel J Scheidig
Sulfate acts as phosphate analog on the monomeric catalytic fragment of the CPx-ATPase CopB from Sulfolobus solfataricus.
Journal of molecular biology 2007;369(2):368-85.
2007: Rasa Sukackaite; Arunas Lagunavicius; Kornelijus Stankevicius; Claus Urbanke; Ceslovas Venclovas; Virginijus Siksnys
Restriction endonuclease BpuJI specific for the 5'-CCCGT sequence is related to the archaeal Holliday junction resolvase family.
Nucleic acids research 2007;35(7):2377-89.
2007: Magdalena Kaus-Drobek; Honorata Czapinska; Monika Sokołowska; Gintautas Tamulaitis; Roman H Szczepanowski; Claus Urbanke; Virginijus Siksnys; Matthias Bochtler
Restriction endonuclease MvaI is a monomer that recognizes its target sequence asymmetrically.
Nucleic acids research 2007;35(6):2035-46.
2006: Mindaugas Zaremba; Giedrius Sasnauskas; Claus Urbanke; Virginijus Siksnys
Allosteric communication network in the tetrameric restriction endonuclease Bse634I.
Journal of molecular biology 2006;363(4):800-12.
2006: Giedre Tamulaitiene; Arturas Jakubauskas; Claus Urbanke; Robert Huber; Saulius Grazulis; Virginijus Siksnys
The crystal structure of the rare-cutting restriction enzyme SdaI reveals unexpected domain architecture.
Structure (London, England : 1993) 2006;14(9):1389-400.
2006: Sebastian Breuer; Holger Gerlach; Branko Kolaric; Claus Urbanke; Norbert Opitz; Matthias Geyer
Biochemical indication for myristoylation-dependent conformational changes in HIV-1 Nef.
Biochemistry 2006;45(7):2339-49.
2006: Roman Fedorov; Gregor Witte; Claus Urbanke; Dietmar J Manstein; Ute Curth
3D structure of Thermus aquaticus single-stranded DNA-binding protein gives insight into the functioning of SSB proteins.
Nucleic acids research 2006;34(22):6708-17.
2006: Laura Manelyte; Claus Urbanke; Luis Giron-Monzon; Peter Friedhoff
Structural and functional analysis of the MutS C-terminal tetramerization domain.
Nucleic acids research 2006;34(18):5270-9.
2005: Julie Dam; Carlos A Velikovsky; Roy A Mariuzza; Claus Urbanke; Peter Schuck
Sedimentation velocity analysis of heterogeneous protein-protein interactions: Lamm equation modeling and sedimentation coefficient distributions c(s).
Biophysical journal 2005;89(1):619-34.
2005: Mindaugas Zaremba; Giedrius Sasnauskas; Claus Urbanke; Virginijus Siksnys
Conversion of the tetrameric restriction endonuclease Bse634I into a dimer: oligomeric structure-stability-function correlations.
Journal of molecular biology 2005;348(2):459-78.
2005: Christopher Bräsen; Claus Urbanke; Peter Schönheit
A novel octameric AMP-forming acetyl-CoA synthetase from the hyperthermophilic crenarchaeon Pyrobaculum aerophilum.
FEBS letters 2005;579(2):477-82.
2005: Ozkan Yildiz; Masao Doi; Irene Yujnovsky; Luca Cardone; Alex Berndt; Sven Hennig; Sabrina Schulze; Claus Urbanke; Paolo Sassone-Corsi; Eva Wolf
Crystal structure and interactions of the PAS repeat region of the Drosophila clock protein PERIOD.
Molecular cell 2005;17(1):69-82.
2005: Claus Urbanke; Gregor Witte; Ute Curth
Sedimentation velocity method in the analytical ultracentrifuge for the study of protein-protein interactions.
Methods in molecular biology (Clifton, N.J.) 2005;305():101-14.
2005: Gregor Witte; Claus Urbanke; Ute Curth
Single-stranded DNA-binding protein of Deinococcus radiodurans: a biophysical characterization.
Nucleic acids research 2005;33(5):1662-70.
2004: Mani Maheswaran; Claus Urbanke; Karl Forchhammer
Complex formation and catalytic activation by the PII signaling protein of N-acetyl-L-glutamate kinase from Synechococcus elongatus strain PCC 7942.
The Journal of biological chemistry 2004;279(53):55202-10.
2004: Thomas Hansen; Claus Urbanke; Peter Schönheit
Bifunctional phosphoglucose/phosphomannose isomerase from the hyperthermophilic archaeon Pyrobaculum aerophilum.
Extremophiles : life under extreme conditions 2004;8(6):507-12.
2004: Klaus Dreisewerd; Andreas Rohlfing; Beatrice Spottke; Claus Urbanke; Werner Henkel
Characterization of whole fibril-forming collagen proteins of types I, III, and V from fetal calf skin by infrared matrix-assisted laser desorption ionization mass spectrometry.
Analytical chemistry 2004;76(13):3482-91.
2004: Mindaugas Zaremba; Claus Urbanke; Stephen E Halford; Virginijus Siksnys
Generation of the BfiI restriction endonuclease from the fusion of a DNA recognition domain to a non-specific nuclease from the phospholipase D superfamily.
Journal of molecular biology 2004;336(1):81-92.
2003: Gregor Witte; Claus Urbanke; Ute Curth
DNA polymerase III chi subunit ties single-stranded DNA binding protein to the bacterial replication machinery.
Nucleic acids research 2003;31(15):4434-40.
2003: Géraldine F Drevon; Claus Urbanke; Alan Russell
Enzyme-containing Michael-adduct-based coatings.
Biomacromolecules 2003;4(3):675-82.
2002: Wolf-Dieter Schubert; Claus Urbanke; Thilo Ziehm; Viola Beier; Matthias Machner; Eugen Domann; Jürgen Wehland; Trinad Chakraborty; Dirk W Heinz
Structure of internalin, a major invasion protein of Listeria monocytogenes, in complex with its human receptor E-cadherin.
Cell 2002;111(6):825-36.
2002: Mirko Glinski; Claus Urbanke; Till Hornbogen; Rainer Zocher
Enniatin synthetase is a monomer with extended structure: evidence for an intramolecular reaction mechanism.
Archives of microbiology 2002;178(4):267-73.
2002: Marco Landwehr; Ute Curth; Claus Urbanke
A dimeric mutant of the homotetrameric single-stranded DNA binding protein from Escherichia coli.
Biological chemistry 2002;383(9):1325-33.
2002: Sylke Meyer; Claus Urbanke; Elmar Wahle
Equilibrium studies on the association of the nuclear poly(A) binding protein with poly(A) of different lengths.
Biochemistry 2002;41(19):6082-9.
2002: Vera Pingoud; Elena Kubareva; Gudrun Stengel; Peter Friedhoff; Janusz Bujnicki; Claus Urbanke; Anna Sudina; Alfred Pingoud
Evolutionary relationship between different subgroups of restriction endonucleases.
The Journal of biological chemistry 2002;277(16):14306-14.
2002: Christoph Kalthoff; Jürgen Alves; Claus Urbanke; Ruth Knorr; Ernst J Ungewickell
Unusual structural organization of the endocytic proteins AP180 and epsin 1.
The Journal of biological chemistry 2002;277(10):8209-16.
2002: Saulius Grazulis; Markus Deibert; Renata Rimseliene; Remigijus Skirgaila; Giedrius Sasnauskas; Arunas Lagunavicius; Vladimir Repin; Claus Urbanke; Robert Huber; Virginijus Siksnys
Crystal structure of the Bse634I restriction endonuclease: comparison of two enzymes recognizing the same DNA sequence.
Nucleic acids research 2002;30(4):876-85.
2001: Matthias Machner; Claus Urbanke; M Barzik; Sonja Otten; Antonio S Sechi; Jürgen Wehland; Dirk W Heinz
ActA from Listeria monocytogenes can interact with up to four Ena/VASP homology 1 domains simultaneously.
The Journal of biological chemistry 2001;276(43):40096-103.
2001: Claus Urbanke; J Wray
A fluorescence temperature-jump study of conformational transitions in myosin subfragment 1.
The Biochemical journal 2001;358(Pt 1):165-73.
2000: Christian Korn; Gregor Meiss; Frank-Ulrich Gast; Oleg Gimadutdinow; Claus Urbanke; Alfred Pingoud
Genetic engineering of Escherichia coli to produce a 1:1 complex of the anabaena sp. PCC 7120 nuclease NucA and its inhibitor NuiA.
Gene 2000;253(2):221-9.
2000: Janine Genschel; Ute Curth; Claus Urbanke
Interaction of E. coli single-stranded DNA binding protein (SSB) with exonuclease I. The carboxy-terminus of SSB is the recognition site for the nuclease.
Biological chemistry 2000;381(3):183-92.
1999: Werner Henkel; T Vogl; H Echner; W Voelter; Claus Urbanke; Detlev Schleuder; Jürgen Rauterberg
Synthesis and folding of native collagen III model peptides.
Biochemistry 1999;38(41):13610-22.
1999: Virginijus Siksnys; Remigijus Skirgaila; Giedrius Sasnauskas; Claus Urbanke; D Cherny; Saulius Grazulis; Robert Huber
The Cfr10I restriction enzyme is functional as a tetramer.
Journal of molecular biology 1999;291(5):1105-18.
1998: C Schulze; Albert Jeltsch; I Franke; Claus Urbanke; Alfred Pingoud
Crosslinking the EcoRV restriction endonuclease across the DNA-binding site reveals transient intermediates and conformational changes of the enzyme during DNA binding and catalytic turnover.
The EMBO journal 1998;17(22):6757-66.
1998: Leslie E Carlini; Ute Curth; B Kindler; Claus Urbanke; R D Porter
Identification of amino acids stabilizing the tetramerization of the single stranded DNA binding protein from Escherichia coli.
FEBS letters 1998;430(3):197-200.
1998: I Franke; Gregor Meiss; D Blecher; Oleg Gimadutdinow; Claus Urbanke; Alfred Pingoud
Genetic engineering, production and characterisation of monomeric variants of the dimeric Serratia marcescens endonuclease.
FEBS letters 1998;425(3):517-22.
1998: Gregor Meiss; I Franke; Oleg Gimadutdinow; Claus Urbanke; Alfred Pingoud
Biochemical characterization of Anabaena sp. strain PCC 7120 non-specific nuclease NucA and its inhibitor NuiA.
European journal of biochemistry / FEBS 1998;251(3):924-34.
1997: G Webster; Janine Genschel; Ute Curth; Claus Urbanke; C Kang; Rolf Hilgenfeld
A common core for binding single-stranded DNA: structural comparison of the single-stranded DNA-binding proteins (SSB) from E. coli and human mitochondria.
FEBS letters 1997;411(2-3):313-6.
1997: C Yang; Ute Curth; Claus Urbanke; C Kang
Crystal structure of human mitochondrial single-stranded DNA binding protein at 2.4 A resolution.
Nature structural biology 1997;4(2):153-7.
1996: Peter Friedhoff; Gregor Meiss; B Kolmes; Uwe Pieper; Oleg Gimadutdinow; Claus Urbanke; Alfred Pingoud
Kinetic analysis of the cleavage of natural and synthetic substrates by the Serratia nuclease.
European journal of biochemistry / FEBS 1996;241(2):572-80.
1996: Ute Curth; Janine Genschel; Claus Urbanke; J Greipel
In vitro and in vivo function of the C-terminus of Escherichia coli single-stranded DNA binding protein.
Nucleic acids research 1996;24(14):2706-11.
1995: C Ebel; W Altekar; J Langowski; Claus Urbanke; E Forest; Giuseppe Zaccai
Solution structure of glyceraldehyde-3-phosphate dehydrogenase from Haloarcula vallismortis.
Biophysical chemistry 1995;54(3):219-27.
1995: Rolf Misselwitz; Karin Welfle; Ute Curth; Claus Urbanke; Heinz Welfle
Stability of Escherichia coli single-stranded DNA binding protein (EcoSSB)
Journal of biomolecular structure & dynamics 1995;12(5):1041-54.
1995: Albert Jeltsch; Jürgen Alves; Claus Urbanke; G Maass; H Eckstein; Z Lianshan; E Bayer; Alfred Pingoud
A dodecapeptide comprising the extended chain-alpha 4 region of the restriction endonuclease EcoRI specifically binds to the EcoRI recognition site.
The Journal of biological chemistry 1995;270(10):5122-9.
1994: A Ebneth; O Schweers; Hubert Thole; U Fagin; Claus Urbanke; G Maass; Heiner Wolfes
Biophysical characterization of the c-Myb DNA-binding domain.
Biochemistry 1994;33(48):14586-93.
1994: J De Vries; Janine Genschel; Claus Urbanke; Hubert Thole; Wilfried Wackernagel
The single-stranded-DNA-binding proteins (SSB) of Proteus mirabilis and Serratia marcescens.
European journal of biochemistry / FEBS 1994;224(2):613-22.
1994: Ute Curth; Claus Urbanke; J Greipel; H Gerberding; V Tiranti; M Zeviani
Single-stranded-DNA-binding proteins from human mitochondria and Escherichia coli have analogous physicochemical properties.
European journal of biochemistry / FEBS 1994;221(1):435-43.
1994: Peter Friedhoff; Oleg Gimadutdinow; T Rüter; Wolfgang Wende; Claus Urbanke; Hubert Thole; Alfred Pingoud
A procedure for renaturation and purification of the extracellular Serratia marcescens nuclease from genetically engineered Escherichia coli.
Protein expression and purification 1994;5(1):37-43.
1993: Leslie E Carlini; R D Porter; Ute Curth; Claus Urbanke
Viability and preliminary in vivo characterization of site-directed mutants of Escherichia coli single-stranded DNA-binding protein.
Molecular microbiology 1993;10(5):1067-75.
1993: Ute Curth; J Greipel; Claus Urbanke; G Maass
Multiple binding modes of the single-stranded DNA binding protein from Escherichia coli as detected by tryptophan fluorescence and site-directed mutagenesis.
Biochemistry 1993;32(10):2585-91.
1992: C Ebel; F Guinet; J Langowski; Claus Urbanke; J Gagnon; Giuseppe Zaccai
Solution studies of elongation factor Tu from the extreme halophile Halobacterium marismortui.
Journal of molecular biology 1992;223(1):361-71.
1991: V Thielking; U Selent; E Köhler; Heiner Wolfes; Uwe Pieper; R Geiger; Claus Urbanke; F K Winkler; Alfred Pingoud
Site-directed mutagenesis studies with EcoRV restriction endonuclease to identify regions involved in recognition and catalysis.
Biochemistry 1991;30(26):6416-22.
1991: A Schaper; Claus Urbanke; G W Kohring; G Maass
Structure and dynamics of the complex of single stranded DNA binding protein of Escherichia coli with circular single stranded DNA of filamentous phages.
Journal of biomolecular structure & dynamics 1991;8(6):1233-50.
1991: A Schaper; Claus Urbanke; G Maass
Salt dependent changes in structure and dynamics of circular single stranded DNA of filamentous phages of Escherichia coli.
Journal of biomolecular structure & dynamics 1991;8(6):1211-32.
1991: Ute Curth; I Bayer; J Greipel; F Mayer; Claus Urbanke; G Maass
Amino acid 55 plays a central role in tetramerization and function of Escherichia coli single-stranded DNA binding protein.
European journal of biochemistry / FEBS 1991;196(1):87-93.
1990: Claus Urbanke; A Schaper
Kinetics of binding of single-stranded DNA binding protein from Escherichia coli to single-stranded nuclei acids.
Biochemistry 1990;29(7):1744-9.
1989: Jürgen Alves; Claus Urbanke; Anja Fliess; G Maass; Alfred Pingoud
Fluorescence stopped-flow kinetics of the cleavage of synthetic oligodeoxynucleotides by the EcoRI restriction endonuclease.
Biochemistry 1989;28(19):7879-88.
1989: R Geiger; T Rüter; Jürgen Alves; Anja Fliess; Heiner Wolfes; V Pingoud; Claus Urbanke; G Maass; Alfred Pingoud; A Düsterhöft
Genetic engineering of EcoRI mutants with altered amino acid residues in the DNA binding site: physicochemical investigations give evidence for an altered monomer/dimer equilibrium for the Gln144Lys145 and Gln144Lys145Lys200 mutants.
Biochemistry 1989;28(6):2667-77.
1989: I Bayer; Anja Fliess; J Greipel; Claus Urbanke; G Maass
Modulation of the affinity of the single-stranded DNA-binding protein of Escherichia coli (E. coli SSB) to poly(dT) by site-directed mutagenesis.
European journal of biochemistry / FEBS 1989;179(2):399-404.
1988: Alfred Pingoud; M Wehrmann; Uwe Pieper; Frank-Ulrich Gast; Claus Urbanke; Jürgen Alves; Juergen Feuerstein; Alfred Wittinghofer
Spectroscopic and hydrodynamic studies reveal structural differences in normal and transforming H-ras gene products.
Biochemistry 1988;27(13):4735-40.
1985: H J Ehbrecht; Alfred Pingoud; Claus Urbanke; G Maass; C Gualerzi
Linear diffusion of restriction endonucleases on DNA.
The Journal of biological chemistry 1985;260(10):6160-6.
1984: Alfred Pingoud; Claus Urbanke; J Alves; H J Ehbrecht; M Zabeau; C Gualerzi
Effect of polyamines and basic proteins on cleavage of DNA by restriction endonucleases.
Biochemistry 1984;23(24):5697-703.
1984: J Langowski; Claus Urbanke; E Schuppe
Construction of a microprocessor-controlled pulsed quench-flow apparatus for the study of fast chemical and biochemical reactions.
Analytical biochemistry 1984;142(1):91-7.
1982: J Alves; Alfred Pingoud; J Langowski; Claus Urbanke; G Maass
Two identical subunits of the EcoRI restriction endonuclease Co-operate in the binding and cleavage of the palindromic substrate.
European journal of biochemistry / FEBS 1982;124(1):139-42.
1981: M Goppelt; J Langowski; Alfred Pingoud; W Haupt; Claus Urbanke; H Mayer; G Maass
The effect of several nucleic acid binding drugs on the cleavage of d(GGAATTCC) and pBR 322 by the Eco RI restriction endonuclease.
Nucleic acids research 1981;9(22):6115-27.
1981: J Langowski; Claus Urbanke; Alfred Pingoud; G Maass
Transient cleavage kinetics of the Eco RI restriction endonuclease measured in a pulsed quench-flow apparatus: enzyme concentration-dependent activity change.
Nucleic acids research 1981;9(14):3483-90.