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Taro Uyeda

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Chemicals & Drugs

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Nobuhisa Umeki; Keiko Hirose; Kohji Ito; Akira Nagasaki; Jun Nakajima; Taro Q P Noguchi; Kiyotaka Tokuraku; Taro Q P Uyeda
Rapid nucleotide exchange renders Asp-11 mutant actins resistant to depolymerizing activity of cofilin, leading to dominant toxicity in vivo.
Taro Q P Noguchi; Kiyotaka Tokuraku; Nobuhisa Umeki; Noriyuki Demizu; Kohji Ito; Atsuko Hikikoshi Iwane; Tomotaka Komori; Toshio Yanagida; Taro Q P Uyeda
G146V mutation at the hinge region of actin reveals a myosin class-specific requirement of actin conformations for motility.
Nobuhisa Umeki; Taro Q P Uyeda
Single restriction enzyme-assisted megaprimer polymerase chain reaction to fuse two DNA sequences on separate cloning vectors.

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2013: Nobuhisa Umeki; Keiko Hirose; Kohji Ito; Akira Nagasaki; Jun Nakajima; Taro Q P Noguchi; Kiyotaka Tokuraku; Taro Q P Uyeda
Rapid nucleotide exchange renders Asp-11 mutant actins resistant to depolymerizing activity of cofilin, leading to dominant toxicity in vivo.
The Journal of biological chemistry 2013;288(3):1739-49.
2012: Taro Q P Noguchi; Kiyotaka Tokuraku; Nobuhisa Umeki; Noriyuki Demizu; Kohji Ito; Atsuko Hikikoshi Iwane; Tomotaka Komori; Toshio Yanagida; Taro Q P Uyeda
G146V mutation at the hinge region of actin reveals a myosin class-specific requirement of actin conformations for motility.
The Journal of biological chemistry 2012;287(29):24339-45.
2011: Nobuhisa Umeki; Taro Q P Uyeda
Single restriction enzyme-assisted megaprimer polymerase chain reaction to fuse two DNA sequences on separate cloning vectors.
Analytical biochemistry 2011;414(2):309-11.
2011: Taro Q P Uyeda; Yoshiaki Iwadate; Akira Nagasaki; Nobuhisa Umeki; Shigehiko Yumura
Stretching actin filaments within cells enhances their affinity for the myosin II motor domain.
PloS one 2011;6(10):e26200.
2010: Kenji Murakami; Takuo Yasunaga; Taro Q P Noguchi; Yuki Gomibuchi; Kien Xuan NGO; Taro Q P Uyeda; Takeyuki Wakabayashi
Structural basis for actin assembly, activation of ATP hydrolysis, and delayed phosphate release.
Cell 2010;143(2):275-87.
2010: Sosuke Iwai; Taro Q P Uyeda
Myosin-actin interaction in Dictyostelium cells revealed by GFP-based strain sensor and validated linear spectral unmixing.
Cytometry. Part A : the journal of the International Society for Analytical Cytology 2010;77(8):743-50.
2010: Taro Q P Noguchi; Ryo Toya; Hironori Ueno; Kiyotaka Tokuraku; Taro Q P Uyeda
Screening of novel dominant negative mutant actins using glycine targeted scanning identifies G146V actin that cooperatively inhibits cofilin binding.
Biochemical and biophysical research communications 2010;396(4):1006-11.
2010: Noriyuki Suetsugu; Noboru Yamada; Takatoshi Kagawa; Hisashi Yonekura; Taro Q P Uyeda; Akeo Kadota; Masamitsu Wada
Two kinesin-like proteins mediate actin-based chloroplast movement in Arabidopsis thaliana.
Proceedings of the National Academy of Sciences of the United States of America 2010;107(19):8860-5.
2010: You Hachikubo; Sosuke Iwai; Taro Q P Uyeda
Photoregulated assembly/disassembly of DNA-templated protein arrays using modified oligonucleotide carrying azobenzene side chains.
Biotechnology and bioengineering 2010;106(1):1-8.
2010: Taro Q P Noguchi; Yuki Gomibuchi; Kenji Murakami; Hironori Ueno; Keiko Hirose; Takeyuki Wakabayashi; Taro Q P Uyeda
Dominant negative mutant actins identified in flightless Drosophila can be classified into three classes.
The Journal of biological chemistry 2010;285(7):4337-47.
2010: Hideyo Takatsuki; Kevin M Rice; Shinichi Asano; B Scott Day; Mizuki Hino; Kazuhiro Oiwa; Ryoki Ishikawa; Yuichi Hiratsuka; Taro Q P Uyeda; Kazuhiro Kohama; Eric R Blough
Utilization of myosin and actin bundles for the transport of molecular cargo.
Small (Weinheim an der Bergstrasse, Germany) 2010;6(3):452-7.
2010: Reiko Onuki-Nagasaki; Akira Nagasaki; Kazumi Hakamada; Taro Q P Uyeda; Satoshi Fujita; Masato Miyake; Jun Miyake
Transfection microarrays for high-throughput phenotypic screening of genes involved in cell migration.
Methods in molecular biology (Clifton, N.J.) 2010;629():193-203.
2009: Masamitsu Kanada; Akira Nagasaki; Taro Q P Uyeda
Stabilization of anaphase midzone microtubules is regulated by Rho during cytokinesis in human fibrosarcoma cells.
Experimental cell research 2009;315(16):2705-14.
2009: Akira Nagasaki; Reiko Nagasaki; Satoshi Fujita; Taro Q P Uyeda
[Genome-wide screening methods for genes involved in cell migration].
Seikagaku. The Journal of Japanese Biochemical Society 2009;81(5):381-6.
2009: Kiyotaka Tokuraku; Rika Kurogi; Ryo Toya; Taro Q P Uyeda
Novel mode of cooperative binding between myosin and Mg2+ -actin filaments in the presence of low concentrations of ATP.
Journal of molecular biology 2009;386(1):149-62.
2009: Takashi Kamei; Haruhisa Akiyama; Hisayuki Morii; Nobuyuki Tamaoki; Taro Q P Uyeda
Visible-light photocontrol of (E)/(Z) isomerization of the 4-(dimethylamino)azobenzene pseudo-nucleotide unit incorporated into an oligonucleotide and DNA hybridization in aqueous media.
Nucleosides, nucleotides & nucleic acids 2009;28(1):12-28.
2008: Yukako Asano; Akira Nagasaki; Taro Q P Uyeda
Correlated waves of actin filaments and PIP3 in Dictyostelium cells.
Cell motility and the cytoskeleton 2008;65(12):923-34.
2008: Sosuke Iwai; Taro Q P Uyeda
Visualizing myosin-actin interaction with a genetically-encoded fluorescent strain sensor.
Proceedings of the National Academy of Sciences of the United States of America 2008;105(44):16882-7.
2008: Akira Nagasaki; Taro Q P Uyeda
Chemotaxis-mediated scission contributes to efficient cytokinesis in Dictyostelium.
Cell motility and the cytoskeleton 2008;65(11):896-903.
2008: Reiko Onuki-Nagasaki; Akira Nagasaki; Kazumi Hakamada; Taro Q P Uyeda; Satoshi Fujita; Masato Miyake; Jun Miyake
On-chip screening method for cell migration genes based on a transfection microarray.
Lab on a chip 2008;8(9):1502-6.
2008: Masatsune Tsujioka; Kunito Yoshida; Akira Nagasaki; Shigenobu Yonemura; Annette Müller-Taubenberger; Taro Q P Uyeda
Overlapping functions of the two talin homologues in Dictyostelium.
Eukaryotic cell 2008;7(5):906-16.
2008: Akira Nagasaki; Taro Q P Uyeda
Screening of genes involved in cell migration in Dictyostelium.
Experimental cell research 2008;314(5):1136-46.
2008: Shu Taira; Yong-Zhong Du; Yuichi Hiratsuka; Taro Q P Uyeda; Noboru Yumoto; Masato Kodaka
Loading and unloading of molecular cargo by DNA-conjugated microtubule.
Biotechnology and bioengineering 2008;99(3):734-9.
2008: Akira Nagasaki; Kimiko Inotsume; Masamitsu Kanada; Taro Q P Uyeda
Phospholipase D is essential for keratocyte-like migration of NBT-II cells.
Cell structure and function 2008;33(1):27-33.
2008: Masamitsu Kanada; Akira Nagasaki; Taro Q P Uyeda
Novel functions of Ect2 in polar lamellipodia formation and polarity maintenance during "contractile ring-independent" cytokinesis in adherent cells.
Molecular biology of the cell 2008;19(1):8-16.
2007: Taro Q P Noguchi; Noriko Kanzaki; Hironori Ueno; Keiko Hirose; Taro Q P Uyeda
A novel system for expressing toxic actin mutants in Dictyostelium and purification and characterization of a dominant lethal yeast actin mutant.
The Journal of biological chemistry 2007;282(38):27721-7.
2007: Akira Nagasaki; Masamitsu Kanada; Taro Q P Uyeda
A novel shRNA vector that enables rapid selection and identification of knockdown cells.
Plasmid 2007;58(2):190-4.
2006: Shu Taira; Yong-Zhong Du; Yuichi Hiratsuka; Kaoru Konishi; Tai Kubo; Taro Q P Uyeda; Noboru Yumoto; Masato Kodaka
Selective detection and transport of fully matched DNA by DNA-loaded microtubule and kinesin motor protein.
Biotechnology and bioengineering 2006;95(3):533-8.
2006: Akiko Nomura; Taro Q P Uyeda; Noboru Yumoto; Yoshiro Tatsu
Photo-control of kinesin-microtubule motility using caged peptides derived from the kinesin C-terminus domain.
Chemical communications (Cambridge, England) 2006;(34):3588-90.
2006: Yuichi Hiratsuka; Makoto Miyata; Tetsuya Tada; Taro Q P Uyeda
A microrotary motor powered by bacteria.
Proceedings of the National Academy of Sciences of the United States of America 2006;103(37):13618-23.
2006: Kaoru Konishi; Taro Q P Uyeda; Tai Kubo
Genetic engineering of a Ca(2+) dependent chemical switch into the linear biomotor kinesin.
FEBS letters 2006;580(15):3589-94.
2006: Miki Hirabayashi; Shu Taira; Suzuko Kobayashi; Kaoru Konishi; Kaoru Katoh; Yuichi Hiratsuka; Masato Kodaka; Taro Q P Uyeda; Noboru Yumoto; Tai Kubo
Malachite green-conjugated microtubules as mobile bioprobes selective for malachite green aptamers with capturing/releasing ability.
Biotechnology and bioengineering 2006;94(3):473-80.
2006: Hisashi Yonekura; Akiko Nomura; Hitomi Ozawa; Yoshiro Tatsu; Noboru Yumoto; Taro Q P Uyeda
Mechanism of tail-mediated inhibition of kinesin activities studied using synthetic peptides.
Biochemical and biophysical research communications 2006;343(2):420-7.
2006: Noriko Kanzaki; Taro Q P Uyeda; Kazuo Onuma
Intermolecular interaction of actin revealed by a dynamic light scattering technique.
The journal of physical chemistry. B 2006;110(6):2881-7.
2005: Md Shahjahan P Siddique; George Mogami; Takashi Miyazaki; Eisaku Katayama; Taro Q P Uyeda; Makoto Suzuki
Cooperative structural change of actin filaments interacting with activated myosin motor domain, detected with copolymers of pyrene-labeled actin and acto-S1 chimera protein.
Biochemical and biophysical research communications 2005;337(4):1185-91.
2005: Shigehiko Yumura; Masashi Yoshida; Venkaiah Betapudi; Lucila Licate; Yoshiaki Iwadate; Akira Nagasaki; Taro Q P Uyeda; Thomas T Egelhoff
Multiple myosin II heavy chain kinases: roles in filament assembly control and proper cytokinesis in Dictyostelium.
Molecular biology of the cell 2005;16(9):4256-66.
2005: Masamitsu Kanada; Akira Nagasaki; Taro Q P Uyeda
Adhesion-dependent and contractile ring-independent equatorial furrowing during cytokinesis in mammalian cells.
Molecular biology of the cell 2005;16(8):3865-72.
2005: Md Shahjahan P Siddique; Takashi Miyazaki; Eisaku Katayama; Taro Q P Uyeda; Makoto Suzuki
Evidence against essential roles for subdomain 1 of actin in actomyosin sliding movements.
Biochemical and biophysical research communications 2005;332(2):474-81.
2005: Yuichi Hiratsuka; Makoto Miyata; Taro Q P Uyeda
Living microtransporter by uni-directional gliding of Mycoplasma along microtracks.
Biochemical and biophysical research communications 2005;331(1):318-24.
2005: Yong-Zhong Du; Yuichi Hiratsuka; Shu Taira; Masaru Eguchi; Taro Q P Uyeda; Noboru Yumoto; Masato Kodaka
Motor protein nano-biomachine powered by self-supplying ATP.
Chemical communications (Cambridge, England) 2005;(16):2080-2.
2005: Francisco Rivero; tetsuya muramoto; Ann-Kathrin Meyer; Hideko Urushihara; Taro Q P Uyeda; Chikako Kitayama
A comparative sequence analysis reveals a common GBD/FH3-FH1-FH2-DAD architecture in formins from Dictyostelium, fungi and metazoa.
BMC genomics 2005;6():28.
2004: Yukako Asano; Takafumi Mizuno; Takahide Kon; Akira Nagasaki; Kazuo Sutoh; Taro Q P Uyeda
Keratocyte-like locomotion in amiB-null Dictyostelium cells.
Cell motility and the cytoskeleton 2004;59(1):17-27.
2004: Taro Q P Uyeda; Akira Nagasaki
Variations on a theme: the many modes of cytokinesis.
Current opinion in cell biology 2004;16(1):55-60.
2004: Akira Nagasaki; Taro Q P Uyeda
DWWA, a novel protein containing two WW domains and an IQ motif, is required for scission of the residual cytoplasmic bridge during cytokinesis in Dictyostelium.
Molecular biology of the cell 2004;15(2):435-46.
2004: Hiroshi Tokumitsu; Naoya Hatano; Hiroyuki Inuzuka; Yumi Ishikawa; Taro Q P Uyeda; Janet L Smith; Ryoji Kobayashi
Regulatory mechanism of Dictyostelium myosin light chain kinase A.
The Journal of biological chemistry 2004;279(1):42-50.
2004: Taro Q P Uyeda; Akira Nagasaki; Shigehiko Yumura
Multiple parallelisms in animal cytokinesis.
International review of cytology 2004;240():377-432.
2004: Makoto Hibi; Akira Nagasaki; Masayuki Takahashi; Akihiko Yamagishi; Taro Q P Uyeda
Dictyostelium discoideum talin A is crucial for myosin II-independent and adhesion-dependent cytokinesis.
Journal of muscle research and cell motility 2004;25(2):127-40.
2003: Kohji Ito; Taro Q P Uyeda; Yoshikazu Suzuki; Kazuo Sutoh; Keiichi Yamamoto
Requirement of domain-domain interaction for conformational change and functional ATP hydrolysis in myosin.
The Journal of biological chemistry 2003;278(33):31049-57.
2003: Yuichi Hiratsuka; Taro Q P Uyeda
[Motor proteins as nano actuators].
Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme 2003;48(11 Suppl):1586-94.
2003: Chikako Kitayama; Taro Q P Uyeda
ForC, a novel type of formin family protein lacking an FH1 domain, is involved in multicellular development in Dictyostelium discoideum.
Journal of cell science 2003;116(Pt 4):711-23.
2003: Shigehiko Yumura; Taro Q P Uyeda
Myosins and cell dynamics in cellular slime molds.
International review of cytology 2003;224():173-225.
2002: Keiichi Yokoyama; Yuichi Hiratuka; Erika Akimaru; Keiko Hirose; Taro Q P Uyeda; Makoto Suzuki
Design and functional analysis of actomyosin motor domain chimera proteins.
Biochemical and biophysical research communications 2002;299(5):825-31.
2002: Akira Nagasaki; Go Itoh; Shigehiko Yumura; Taro Q P Uyeda
Novel myosin heavy chain kinase involved in disassembly of myosin II filaments and efficient cleavage in mitotic dictyostelium cells.
Molecular biology of the cell 2002;13(12):4333-42.
2002: Shi Shu; Xiong Liu; Carole A Parent; Taro Q P Uyeda; Edward D Korn
Tail chimeras of Dictyostelium myosin II support cytokinesis and other myosin II activities but not full development.
Journal of cell science 2002;115(Pt 22):4237-49.
2002: Reiko Onuki; Akira Nagasaki; Hiroaki Kawasaki; Tadashi Baba; Taro Q P Uyeda; Kazunari Taira
Confirmation by FRET in individual living cells of the absence of significant amyloid beta -mediated caspase 8 activation.
Proceedings of the National Academy of Sciences of the United States of America 2002;99(23):14716-21.
2002: Taro Q P Uyeda; Kiyotaka Tokuraku; Kuniyoshi Kaseda; Martin R Webb; Bruce Patterson
Evidence for a novel, strongly bound acto-S1 complex carrying ADP and phosphate stabilized in the G680V mutant of Dictyostelium myosin II.
Biochemistry 2002;41(30):9525-34.
2002: Akira Nagasaki; Eugenio L de Hostos; Taro Q P Uyeda
Genetic and morphological evidence for two parallel pathways of cell-cycle-coupled cytokinesis in Dictyostelium.
Journal of cell science 2002;115(Pt 10):2241-51.
2002: Taro Q P Uyeda; Bruce Patterson; Leonardo Mendoza; Yuichi Hiratsuka
Amino acids 519-524 of Dictyostelium myosin II form a surface loop that aids actin binding by facilitating a conformational change.
Journal of muscle research and cell motility 2002;23(7-8):685-95.
2001: A Nagasaki; M Hibi; Y Asano; Taro Q P Uyeda
Genetic approaches to dissect the mechanisms of two distinct pathways of cell cycle-coupled cytokinesis in Dictyostelium.
Cell structure and function 2001;26(6):585-91.
2001: Yuichi Hiratsuka; T Tada; Kazuhiro Oiwa; T Kanayama; Taro Q P Uyeda
Controlling the direction of kinesin-driven microtubule movements along microlithographic tracks.
Biophysical journal 2001;81(3):1555-61.
2001: M Takahashi; K Takahashi; Yuichi Hiratsuka; K Uchida; A Yamagishi; Taro Q P Uyeda; M Yazawa
Functional characterization of vertebrate nonmuscle myosin IIB isoforms using Dictyostelium chimeric myosin II.
The Journal of biological chemistry 2001;276(2):1034-40.
2001: Kiyotaka Tokuraku; Taro Q P Uyeda
Phalloidin affects the myosin-dependent sliding velocities of actin filaments in a bound-divalent cation dependent manner.
Journal of muscle research and cell motility 2001;22(4):371-8.
2000: Y Fukui; Taro Q P Uyeda; Chikako Kitayama; Shinya Inoué
How well can an amoeba climb?
Proceedings of the National Academy of Sciences of the United States of America 2000;97(18):10020-5.
2000: X Liu; K Ito; R J Lee; Taro Q P Uyeda
Involvement of tail domains in regulation of Dictyostelium myosin II.
Biochemical and biophysical research communications 2000;271(1):75-81.
2000: Taro Q P Uyeda; Shigehiko Yumura
Molecular biological approaches to study myosin functions in cytokinesis of Dictyostelium.
Microscopy research and technique 2000;49(2):136-44.
2000: Taro Q P Uyeda; Chikako Kitayama; Shigehiko Yumura
Myosin II-independent cytokinesis in Dictyostelium: its mechanism and implications.
Cell structure and function 2000;25(1):1-10.
1999: S Shu; R J Lee; J M LeBlanc-Straceski; Taro Q P Uyeda
Role of myosin II tail sequences in its function and localization at the cleavage furrow in Dictyostelium.
Journal of cell science 1999;112 ( Pt 13)():2195-201.
1999: K Ito; X Liu; Eisaku Katayama; Taro Q P Uyeda
Cooperativity between two heads of dictyostelium myosin II in in vitro motility and ATP hydrolysis.
Biophysical journal 1999;76(2):985-92.
1998: X Liu; K Ito; S Morimoto; A Hikkoshi-Iwane; T Yanagida; Taro Q P Uyeda
Filament structure as an essential factor for regulation of Dictyostelium myosin by regulatory light chain phosphorylation.
Proceedings of the National Academy of Sciences of the United States of America 1998;95(24):14124-9.
1997: Shigehiko Yumura; Taro Q P Uyeda
Transport of myosin II to the equatorial region without its own motor activity in mitotic Dictyostelium cells.
Molecular biology of the cell 1997;8(10):2089-99.
1997: Shigehiko Yumura; Taro Q P Uyeda
Myosin II can be localized to the cleavage furrow and to the posterior region of Dictyostelium amoebae without control by phosphorylation of myosin heavy and light chains.
Cell motility and the cytoskeleton 1997;36(4):313-22.