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Bertus van den Burg

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TOP 3
Eijsink Vincent G H; Gåseidnes Sigrid; Borchert Torben V; van den Burg Bertus
Directed evolution of enzyme stability.
Eijsink Vincent G H; Bjørk Alexandra; Gåseidnes Sigrid; Sirevåg Reidun; Synstad Bjørnar; van den Burg Bertus; Vriend Gert
Rational engineering of enzyme stability.
van den Burg Bertus
Extremophiles as a source for novel enzymes.

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All Publications

2005: Eijsink Vincent G H; Gåseidnes Sigrid; Borchert Torben V; van den Burg Bertus
Directed evolution of enzyme stability.
Biomolecular engineering 2005;22(1-3):21-30.
2004: Eijsink Vincent G H; Bjørk Alexandra; Gåseidnes Sigrid; Sirevåg Reidun; Synstad Bjørnar; van den Burg Bertus; Vriend Gert
Rational engineering of enzyme stability.
Journal of biotechnology 2004;113(1-3):105-20.
2003: van den Burg Bertus
Extremophiles as a source for novel enzymes.
Current opinion in microbiology 2003;6(3):213-8.
2002: van den Burg Bertus; Eijsink Vincent G H
Selection of mutations for increased protein stability.
Current opinion in biotechnology 2002;13(4):333-7.
2002: de Kreij Arno; van den Burg Bertus; Venema Gerard; Vriend Gert; Eijsink Vincent G H; Nielsen Jens E
The effects of modifying the surface charge on the catalytic activity of a thermolysin-like protease.
The Journal of biological chemistry 2002;277(18):15432-8.
2001: de Kreij A; van den Burg B; Veltman O R; Vriend G; Venema G; Eijsink V G
The effect of changing the hydrophobic S1' subsite of thermolysin-like proteases on substrate specificity.
European journal of biochemistry / FEBS 2001;268(18):4985-91.
2000: de Kreij A; Venema G; van den Burg B
Substrate specificity in the highly heterogeneous M4 peptidase family is determined by a small subset of amino acids.
The Journal of biological chemistry 2000;275(40):31115-20.
1999: Van den Burg B; de Kreij A; Van der Veek P; Mansfeld J; Venema G
Characterization of a novel stable biocatalyst obtained by protein engineering.
Biotechnology and applied biochemistry 1999;30 ( Pt 1)():35-40.
1999: Mansfeld J; Vriend G; Van den Burg B; Eijsink V G; Ulbrich-Hofmann R
Probing the unfolding region in a thermolysin-like protease by site-specific immobilization.
Biochemistry 1999;38(26):8240-5.
1998: Vriend G; Berendsen H J; van den Burg B; Venema G; Eijsink V G
Early steps in the unfolding of thermolysin-like proteases.
The Journal of biological chemistry 1998;273(52):35074-7.
1998: Van den Burg B; Eijsink V G; Vriend G; Veltman O R; Venema G
Rendering one autolysis site in Bacillus subtilis neutral protease resistant to cleavage reveals a new fission.
Biotechnology and applied biochemistry 1998;27 ( Pt 2)():125-32.
1998: Veltman O R; Vriend G; Berendsen H J; Van den Burg B; Venema G; Eijsink V G
A single calcium binding site is crucial for the calcium-dependent thermal stability of thermolysin-like proteases.
Biochemistry 1998;37(15):5312-9.
1998: Veltman O R; Eijsink V G; Vriend G; de Kreij A; Venema G; Van den Burg B
Probing catalytic hinge bending motions in thermolysin-like proteases by glycine --> alanine mutations.
Biochemistry 1998;37(15):5305-11.
1998: Van den Burg B; Vriend G; Veltman O R; Venema G; Eijsink V G
Engineering an enzyme to resist boiling.
Proceedings of the National Academy of Sciences of the United States of America 1998;95(5):2056-60.
1997: Nauta A; van den Burg B; Karsens H; Venema G; Kok J
Design of thermolabile bacteriophage repressor mutants by comparative molecular modeling.
Nature biotechnology 1997;15(10):980-3.
1997: Veltman O R; Vriend G; Hardy F; Mansfeld J; van den Burg B; Venema G; Eijsink V G
Mutational analysis of a surface area that is critical for the thermal stability of thermolysin-like proteases.
European journal of biochemistry / FEBS 1997;248(2):433-40.
1997: Mansfeld J; Vriend G; Dijkstra B W; Veltman O R; Van den Burg B; Venema G; Ulbrich-Hofmann R; Eijsink V G
Extreme stabilization of a thermolysin-like protease by an engineered disulfide bond.
The Journal of biological chemistry 1997;272(17):11152-6.
1997: Veltman O R; Vriend G; van den Burg B; Hardy F; Venema G; Eijsink V G
Engineering thermolysin-like proteases whose stability is largely independent of calcium.
FEBS letters 1997;405(2):241-4.
1996: Veltman O R; Vriend G; Middelhoven P J; van den Burg B; Venema G; Eijsink V G
Analysis of structural determinants of the stability of thermolysin-like proteases by molecular modelling and site-directed mutagenesis.
Protein engineering 1996;9(12):1181-9.
1994: Van den Burg B; Dijkstra B W; Vriend G; Van der Vinne B; Venema G; Eijsink V G
Protein stabilization by hydrophobic interactions at the surface.
European journal of biochemistry / FEBS 1994;220(3):981-5.
1993: van den Burg B; Dijkstra B W; van der Vinne B; Stulp B K; Eijsink V G; Venema G
Introduction of disulfide bonds into Bacillus subtilis neutral protease.
Protein engineering 1993;6(5):521-7.
1992: Eijsink V G; Vriend G; van der Vinne B; Hazes B; van den Burg B; Venema G
Effects of changing the interaction between subdomains on the thermostability of Bacillus neutral proteases.
Proteins 1992;14(2):224-36.
1992: Eijsink V G; Vriend G; Van der Zee J R; Van den Burg B; Venema G
Increasing the thermostability of the neutral proteinase of Bacillus stearothermophilus by improvement of internal hydrogen-bonding.
The Biochemical journal 1992;285 ( Pt 2)():625-8.
1992: Eijsink V G; Dijkstra B W; Vriend G; van der Zee J R; Veltman O R; van der Vinne B; van den Burg B; Kempe S; Venema G
The effect of cavity-filling mutations on the thermostability of Bacillus stearothermophilus neutral protease.
Protein engineering 1992;5(5):421-6.
1992: Eijsink V G; Vriend G; van den Burg B; van der Zee J R; Venema G
Increasing the thermostability of a neutral protease by replacing positively charged amino acids in the N-terminal turn of alpha-helices.
Protein engineering 1992;5(2):165-70.
1992: Eijsink V G; Vriend G; van den Burg B; van der Zee J R; Veltman O R; Stulp B K; Venema G
Introduction of a stabilizing 10 residue beta-hairpin in Bacillus subtilis neutral protease.
Protein engineering 1992;5(2):157-63.
1991: Vriend G; Berendsen H J; van der Zee J R; van den Burg B; Venema G; Eijsink V G
Stabilization of the neutral protease of Bacillus stearothermophilus by removal of a buried water molecule.
Protein engineering 1991;4(8):941-5.
1991: Eijsink V G; van den Burg B; Venema G
High performance affinity chromatography of Bacillus neutral proteases.
Biotechnology and applied biochemistry 1991;14(3):275-83.
1991: van den Burg B; Enequist H G; van der Haar M E; Eijsink V G; Stulp B K; Venema G
A highly thermostable neutral protease from Bacillus caldolyticus: cloning and expression of the gene in Bacillus subtilis and characterization of the gene product.
Journal of bacteriology 1991;173(13):4107-15.
1991: Eijsink V G; van den Burg B; Vriend G; Berendsen H J; Venema G
Thermostability of Bacillus subtilis neutral protease.
Biochemistry international 1991;24(3):517-25.
1991: Eijsink V G; van der Zee J R; van den Burg B; Vriend G; Venema G
Improving the thermostability of the neutral protease of Bacillus stearothermophilus by replacing a buried asparagine by leucine.
FEBS letters 1991;282(1):13-6.
1990: van den Burg B; Eijsink V G; Stulp B K; Venema G
Identification of autodigestion target sites in Bacillus subtilis neutral proteinase.
The Biochemical journal 1990;272(1):93-7.
1990: Eijsink V G; Vriend G; Van Den Burg B; Venema G; Stulp B K
Contribution of the C-terminal amino acid to the stability of Bacillus subtilis neutral protease.
Protein engineering 1990;4(1):99-104.
1989: Van Den Burg B; Eijsink V G; Stulp B K; Venema G
One-step affinity purification of Bacillus neutral proteases using bacitracin-silica.
Journal of biochemical and biophysical methods 1989;18(3):209-19.

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