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HM Verheij
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28
Egmond, Maarten
19
Dekker, Nick
13
Slotboom, AJ
9
Dijkstra, Bauke
8
Tommassen, Jan
7
Cox, RC
7
Kuipers, Oscar
7
Simons, JW
6
Mannesse, Maurice
6
Boots, Jan-Willem
6
van der Hijden, HT
6
van Kampen, MD
5
Ubarretxena-Belandia, I
5
Kalk, Kor
4
Janssen, Marcel
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All Publications
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2000: Kingma R L; Fragiathaki M; Snijder H J; Dijkstra B W; Verheij H M; Dekker N; Egmond M R
Unusual catalytic triad of Escherichia coli outer membrane phospholipase A.
Biochemistry 2000;39(33):10017-22.
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2000: Yu B Z; Janssen M J; Verheij H M; Jain M K
Control of the chemical step by leucine-31 of pancreatic phospholipase A2.
Biochemistry 2000;39(19):5702-11.
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1999: Koops B C; Papadimou E; Verheij H M; Slotboom A J; Egmond M R
Activity and stability of chemically modified Candida antarctica lipase B adsorbed on solid supports.
Applied microbiology and biotechnology 1999;52(6):791-6.
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1999: Snijder H J; Ubarretxena-Belandia I; Blaauw M; Kalk K H; Verheij H M; Egmond M R; Dekker N; Dijkstra B W
Structural evidence for dimerization-regulated activation of an integral membrane phospholipase.
Nature 1999;401(6754):717-21.
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1999: Janssen M J; Burghout P J; Verheij H M; Slotboom A J; Egmond M R
Introduction of a C-terminal aromatic sequence from snake venom phospholipases A2 into the porcine pancreatic isozyme dramatically changes the interfacial kinetics.
European journal of biochemistry / FEBS 1999;263(3):782-8.
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1999: van Kampen M D; Verheij H M; Egmond M R
Modifying the substrate specificity of staphylococcal lipases.
Biochemistry 1999;38(29):9524-32.
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1999: Janssen M J; van de Wiel W A; Beiboer S H; van Kampen M D; Verheij H M; Slotboom A J; Egmond M R
Catalytic role of the active site histidine of porcine pancreatic phospholipase A2 probed by the variants H48Q, H48N and H48K.
Protein engineering 1999;12(6):497-503.
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1999: Ubarretxena-Belandia I; Hozeman L; van der Brink-van der Laan E; Pap E H; Egmond M R; Verheij H M; Dekker N
Outer membrane phospholipase A is dimeric in phospholipid bilayers: a cross-linking and fluorescence resonance energy transfer study.
Biochemistry 1999;38(22):7398-405.
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1999: Dekker N; Tommassen J; Verheij H M
Bacteriocin release protein triggers dimerization of outer membrane phospholipase A in vivo.
Journal of bacteriology 1999;181(10):3281-3.
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1999: Simons J W; Cox R C; Egmond M R; Verheij H M
Rational design of alpha-keto triglyceride analogues as inhibitors for Staphylococcus hyicus lipase.
Biochemistry 1999;38(19):6346-51.
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1999: Prompers J J; van Noorloos B; Mannesse M L; Groenewegen A; Egmond M R; Verheij H M; Hilbers C W; Pepermans H A
NMR studies of Fusarium solani pisi cutinase in complex with phosphonate inhibitors.
Biochemistry 1999;38(19):5982-94.
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1999: Janssen M J; Verheij H M; Slotboom A J; Egmond M R
Engineering the disulphide bond patterns of secretory phospholipases A2 into porcine pancreatic isozyme. The effects on folding, stability and enzymatic properties.
European journal of biochemistry / FEBS 1999;261(1):197-207.
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1999: Ubarretxena-Belandia I; Cox R C; Dijkman R; Egmond M R; Verheij H M; Dekker N
Half-of-the-sites reactivity of outer-membrane phospholipase A against an active-site-directed inhibitor.
European journal of biochemistry / FEBS 1999;260(3):794-800.
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1999: Flipsen J A; van Schaick M A; Dijkman R; van der Hijden H T; Verheij H M; Egmond M R
Interfacial binding of cutinase rather than its catalytic activity determines the steady state interfacial tension during oil drop lipid hydrolysis.
Chemistry and physics of lipids 1999;97(2):181-91.
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1999: Simons J W; van Kampen M D; Ubarretxena-Belandia I; Cox R C; Alves dos Santos C M; Egmond M R; Verheij H M
Identification of a calcium binding site in Staphylococcus hyicus lipase: generation of calcium-independent variants.
Biochemistry 1999;38(1):2-10.
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1998: Brok R G; Boots A P; Dekker N; Verheij H M; Tommassen J
Sequence comparison of outer membrane phospholipases A: implications for structure and for the catalytic mechanism.
Research in microbiology 1998;149(10):703-10.
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1998: Ubarretxena-Belandia I; Boots J W; Verheij H M; Dekker N
Role of the cofactor calcium in the activation of outer membrane phospholipase A.
Biochemistry 1998;37(45):16011-8.
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1998: Flipsen J A; Kramer R A; van Duijnhoven J P; van der Hijden H T; Egmond M R; Verheij H M
Cutinase binding and activity at the triolein-water interface monitored by oil drop tensiometry.
Chemistry and physics of lipids 1998;95(2):169-80.
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1998: Boekema E J; Stuart M; Koning R I; Keegstra W; Brisson A; Verheij H M; Dekker N
A 7.4-A projection structure of outer membrane phospholipase A from Escherichia coli by electron crystallography.
Journal of structural biology 1998;123(1):67-71.
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1998: van Kampen M D; Simons J W; Dekker N; Egmond M R; Verheij H M
The phospholipase activity of Staphylococcus hyicus lipase strongly depends on a single Ser to Val mutation.
Chemistry and physics of lipids 1998;93(1-2):39-45.
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1998: Simons J W; Götz F; Egmond M R; Verheij H M
Biochemical properties of staphylococcal (phospho)lipases.
Chemistry and physics of lipids 1998;93(1-2):27-37.
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1998: Lang D A; Mannesse M L; de Haas G H; Verheij H M; Dijkstra B W
Structural basis of the chiral selectivity of Pseudomonas cepacia lipase.
European journal of biochemistry / FEBS 1998;254(2):333-40.
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1998: Simons J W; van Kampen M D; Riel S; Götz F; Egmond M R; Verheij H M
Cloning, purification and characterisation of the lipase from Staphylococcus epidermidis--comparison of the substrate selectivity with those of other microbial lipases.
European journal of biochemistry / FEBS 1998;253(3):675-83.
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1998: van Kampen M D; Dekker N; Egmond M R; Verheij H M
Substrate specificity of Staphylococcus hyicus lipase and Staphylococcus aureus lipase as studied by in vivo chimeragenesis.
Biochemistry 1998;37(10):3459-66.
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1997: Simons J W; Boots J W; Kats M P; Slotboom A J; Egmond M R; Verheij H M
Dissecting the catalytic mechanism of staphylococcal lipases using carbamate substrates: chain length selectivity, interfacial activation, and cofactor dependence.
Biochemistry 1997;36(47):14539-50.
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1997: Dijkman R; Beiboer S H; Verheij H M
An affinity column for phospholipase A2 based on immobilised acylaminophospholipid analogues.
Biochimica et biophysica acta 1997;1347(1):1-8.
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1997: Merck K B; de Cock H; Verheij H M; Tommassen J
Topology of the outer membrane phospholipase A of Salmonella typhimurium.
Journal of bacteriology 1997;179(11):3443-50.
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1997: Mannesse M L; de Haas G H; van der Hijden H T; Egmond M R; Verheij H M
Chiral preference of cutinase in the reaction with phosphonate inhibitors.
Biochemical Society transactions 1997;25(1):165-70.
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1997: Longhi S; Mannesse M; Verheij H M; De Haas G H; Egmond M; Knoops-Mouthuy E; Cambillau C
Crystal structure of cutinase covalently inhibited by a triglyceride analogue.
Protein science : a publication of the Protein Society 1997;6(2):275-86.
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1997: Dekker N; Tommassen J; Lustig A; Rosenbusch J P; Verheij H M
Dimerization regulates the enzymatic activity of Escherichia coli outer membrane phospholipase A.
The Journal of biological chemistry 1997;272(6):3179-84.
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1996: Flipsen J A; van der Hijden H T; Egmond M R; Verheij H M
Action of cutinase at the triolein-water interface. Characterisation of interfacial effects during lipid hydrolysis using the oil-drop tensiometer as a tool to study lipase kinetics.
Chemistry and physics of lipids 1996;84(2):105-15.
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1996: Simons J W; Adams H; Cox R C; Dekker N; Götz F; Slotboom A J; Verheij H M
The lipase from Staphylococcus aureus. Expression in Escherichia coli, large-scale purification and comparison of substrate specificity to Staphylococcus hyicus lipase.
European journal of biochemistry / FEBS 1996;242(3):760-9.
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1996: Brok R G; Belandia I U; Dekker N; Tommassen J; Verheij H M
Escherichia coli outer membrane phospholipase A: role of two serines in enzymatic activity.
Biochemistry 1996;35(24):7787-93.
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1996: Bekkers A C; Slotboom A J; van Willigen G; Akkerman J W; Verheij H M
Targeting of porcine pancreatic phospholipase A2 to human platelets. Introduction of an RGD sequence and acyl-group by chemical modification.
European journal of biochemistry / FEBS 1996;238(1):70-6.
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1996: Mounier C; Franken P A; Verheij H M; Bon C
The anticoagulant effect of the human secretory phospholipase A2 on blood plasma and on a cell-free system is due to a phospholipid-independent mechanism of action involving the inhibition of factor Va.
European journal of biochemistry / FEBS 1996;237(3):778-85.
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1996: Beiboer S H; van den Berg B; Dekker N; Cox R C; Verheij H M
Incorporation of an unnatural amino acid in the active site of porcine pancreatic phospholipase A2. Substitution of histidine by 1,2,4-triazole-3-alanine yields an enzyme with high activity at acidic pH.
Protein engineering 1996;9(4):345-52.
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1995: Brok R G; Dekker N; Gerrits N; Verheij H M; Tommassen J
A conserved histidine residue of Escherichia coli outer-membrane phospholipase A is important for activity.
European journal of biochemistry / FEBS 1995;234(3):934-8.
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1995: Blaauw M; Dekker N; Verheij H M; Kalk K H; Dijkstra B W
Crystallization and preliminary X-ray analysis of outer membrane phospholipase A from Escherichia coli.
FEBS letters 1995;373(1):10-2.
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1995: Mannesse M L; Boots J W; Dijkman R; Slotboom A J; van der Hijden H T; Egmond M R; Verheij H M; de Haas G H
Phosphonate analogues of triacylglycerols are potent inhibitors of lipase.
Biochimica et biophysica acta 1995;1259(1):56-64.
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1995: van den Berg B; Tessari M; de Haas G H; Verheij H M; Boelens R; Kaptein R
Solution structure of porcine pancreatic phospholipase A2.
The EMBO journal 1995;14(17):4123-31.
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1995: Beiboer S H; Franken P A; Cox R C; Verheij H M
An extended binding pocket determines the polar head group specificity of porcine pancreatic phospholipase A2.
European journal of biochemistry / FEBS 1995;231(3):747-53.
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1995: Dekker N; Merck K; Tommassen J; Verheij H M
In vitro folding of Escherichia coli outer-membrane phospholipase A.
European journal of biochemistry / FEBS 1995;232(1):214-9.
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1995: De Haas G H; Dijkman R; Boots J W; Verheij H M
Competitive inhibition of lipolytic enzymes. XI. Estimation of the interfacial dissociation constants of porcine pancreatic phospholipase A2 for substrate and inhibitor in the absence of detergents.
Biochimica et biophysica acta 1995;1257(2):87-95.
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1995: Mannesse M L; Cox R C; Koops B C; Verheij H M; de Haas G H; Egmond M R; van der Hijden H T; de Vlieg J
Cutinase from Fusarium solani pisi hydrolyzing triglyceride analogues. Effect of acyl chain length and position in the substrate molecule on activity and enantioselectivity.
Biochemistry 1995;34(19):6400-7.
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1995: van den Berg B; Tessari M; Boelens R; Dijkman R; de Haas G H; Kaptein R; Verheij H M
NMR structures of phospholipase A2 reveal conformational changes during interfacial activation.
Nature structural biology 1995;2(5):402-6.
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1995: Boots J W; van Dongen W D; Verheij H M; de Haas G H; Haverkamp J; Slotboom A J
Identification of the active site histidine in Staphylococcus hyicus lipase using chemical modification and mass spectrometry.
Biochimica et biophysica acta 1995;1248(1):27-34.
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1995: Ransac S; Blaauw M; Dijkstra B W; Slotboom A T; Boots J W; Verheij H M
Crystallization and preliminary X-ray analysis of a lipase from Staphylococcus hyicus.
Journal of structural biology 1995;114(2):153-5.
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1995: van den Berg B; Tessari M; Boelens R; Dijkman R; Kaptein R; de Haas G H; Verheij H M
Solution structure of porcine pancreatic phospholipase A2 complexed with micelles and a competitive inhibitor.
Journal of biomolecular NMR 1995;5(2):110-21.
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1994: Inada M; Crowl R M; Bekkers A C; Verheij H; Weiss J
Determinants of the inhibitory action of purified 14-kDa phospholipases A2 on cell-free prothrombinase complex.
The Journal of biological chemistry 1994;269(42):26338-43.
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1994: Mounier C; Vargaftig B B; Franken P A; Verheij H M; Bon C; Touqui L
Platelet secretory phospholipase A2 fails to induce rabbit platelet activation and to release arachidonic acid in contrast with venom phospholipases A2.
Biochimica et biophysica acta 1994;1214(1):88-96.
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1994: Dijkman R; Cox R; van den Berg L; Verheij H M; De Haas G H
Competitive inhibition of lipolytic enzymes. X. Further delineation of the active site of pancreatic phospholipases A2 from pig, ox and horse by comparing the inhibitory power of a number of (R)-2-acylamino phospholipid analogues.
Biochimica et biophysica acta 1994;1212(1):50-8.
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1994: Leuveling Tjeenk M; Bulsink Y B; Slotboom A J; Verheij H M; de Haas G H; Demleitner G; Götz F
Inactivation of Staphylococcus hyicus lipase by hexadecylsulfonyl fluoride: evidence for an active site serine.
Protein engineering 1994;7(4):579-83.
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1994: Brok R G; Brinkman E; van Boxtel R; Bekkers A C; Verheij H M; Tommassen J
Molecular characterization of enterobacterial pldA genes encoding outer membrane phospholipase A.
Journal of bacteriology 1994;176(3):861-70.
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1993: Lugtigheid R B; Nicolaes G A; Veldhuizen E J; Slotboom A J; Verheij H M; De Haas G H
Acylation of porcine pancreatic phospholipase A2 influences penetration and substrate head-group binding, depending on the position of the acylated lysine in the enzyme molecule.
European journal of biochemistry / FEBS 1993;216(2):519-25.
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1993: Thunnissen M M; Franken P A; de Haas G H; Drenth J; Kalk K H; Verheij H M; Dijkstra B W
Crystal structure of a porcine pancreatic phospholipase A2 mutant. A large conformational change caused by the F63V point mutation.
Journal of molecular biology 1993;232(3):839-55.
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1993: Vincent M; Deveer A M; De Haas G H; Verheij H M; Gallay J
Stereospecificity of the interaction of porcine pancreatic phospholipase A2 with micellar and monomeric inhibitors. A time-resolved fluorescence study of the tryptophan residue.
European journal of biochemistry / FEBS 1993;215(3):531-9.
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1993: de Haas G H; Dijkman R; Lugtigheid R B; Dekker N; Van den Berg L; Egmond M R; Verheij H M
Competitive inhibition of lipolytic enzymes. IX. A comparative study on the inhibition of pancreatic phospholipases A2 from different sources by (R)-2-acylamino phospholipid analogues.
Biochimica et biophysica acta 1993;1167(3):281-8.
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1993: Lugtigheid R B; Otten-Kuipers M A; Verheij H M; De Haas G H
Arginine 53 is involved in head-group specificity of the active site of porcine pancreatic phospholipase A2.
European journal of biochemistry / FEBS 1993;213(1):517-22.
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1992: Thunnissen M M; Franken P A; de Haas G H; Drenth J; Kalk K H; Verheij H M; Dijkstra B W
Site-directed mutagenesis and X-ray crystallography of two phospholipase A2 mutants: Y52F and Y73F.
Protein engineering 1992;5(7):597-603.
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1992: Deveer A M; den Ouden A T; Vincent M; Gallay J; Verger R; Egmond M R; Verheij H M; de Haas G H
Competitive inhibition of lipolytic enzymes. VIII: Inhibitor-induced aggregation of porcine pancreatic phospholipase A2.
Biochimica et biophysica acta 1992;1126(1):95-104.
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1992: Deveer A M; Franken P A; Dijkman R; Meeldijk J; Egmond M R; Verheij H M; Verger R; de Haas G H
Competitive inhibition of lipolytic enzymes. VII. The interaction of pancreatic phospholipase A2 with micellar lipid/water interfaces of competitive inhibitors.
Biochimica et biophysica acta 1992;1125(1):73-81.
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1992: van den Berg L; Franken P A; Verheij H M; Dijkman R; de Haas G H
Competitive inhibition of lipolytic enzymes. VI. Inhibition of two human phospholipases A2 by acylamino phospholipid analogues.
Biochimica et biophysica acta 1992;1124(1):66-70.
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1992: Franken P A; Van den Berg L; Huang J; Gunyuzlu P; Lugtigheid R B; Verheij H M; De Haas G H
Purification and characterization of a mutant human platelet phospholipase A2 expressed in Escherichia coli. Cleavage of a fusion protein with cyanogen bromide.
European journal of biochemistry / FEBS 1992;203(1-2):89-98.
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1991: Deveer A M; Dijkman R; Leuveling-Tjeenk M; van den Berg L; Ransac S; Batenburg M; Egmond M; Verheij H M; de Haas G H
A monolayer and bulk study on the kinetic behavior of Pseudomonas glumae lipase using synthetic pseudoglycerides.
Biochemistry 1991;30(41):10034-42.
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1991: Kuipers O P; Vincent M; Brochon J C; Verheij H M; de Haas G H; Gallay J
Insight into the conformational dynamics of specific regions of porcine pancreatic phospholipase A2 from a time-resolved fluorescence study of a genetically inserted single tryptophan residue.
Biochemistry 1991;30(36):8771-85.
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1991: Bekkers A C; Franken P A; Van den Bergh C J; Verbakel J M; Verheij H M; De Haas G H
The use of genetic engineering to obtain efficient production of porcine pancreatic phospholipase A2 by Saccharomyces cerevisiae.
Biochimica et biophysica acta 1991;1089(3):345-51.
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1991: Horrevoets A J; Francke C; Verheij H M; de Haas G H
Activation of reconstituted Escherichia coli outer-membrane phospholipase A by membrane-perturbing peptides results in an increased reactivity towards the affinity label hexadecanesulfonyl fluoride.
European journal of biochemistry / FEBS 1991;198(1):255-61.
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1991: Horrevoets A J; Verheij H M; de Haas G H
Inactivation of Escherichia coli outer-membrane phospholipase A by the affinity label hexadecanesulfonyl fluoride. Evidence for an active-site serine.
European journal of biochemistry / FEBS 1991;198(1):247-53.
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1991: Weiss J; Wright G; Bekkers A C; van den Bergh C J; Verheij H M
Conversion of pig pancreas phospholipase A2 by protein engineering into enzyme active against Escherichia coli treated with the bactericidal/permeability-increasing protein.
The Journal of biological chemistry 1991;266(7):4162-7.
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1991: Bekkers A C; Franken P A; Toxopeus E; Verheij H M; de Haas G H
The importance of glycine-30 for enzymatic activity of phospholipase A2.
Biochimica et biophysica acta 1991;1076(3):374-8.
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1990: Kuipers O P; Franken P A; Hendriks R; Verheij H M; de Haas G H
Function of the fully conserved residues Asp99, Tyr52 and Tyr73 in phospholipase A2.
Protein engineering 1990;4(2):199-204.
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1990: Thunnissen M M; Ab E; Kalk K H; Drenth J; Dijkstra B W; Kuipers O P; Dijkman R; de Haas G H; Verheij H M
X-ray structure of phospholipase A2 complexed with a substrate-derived inhibitor.
Nature 1990;347(6294):689-91.
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1990: Kuipers O P; Kerver J; van Meersbergen J; Vis R; Dijkman R; Verheij H M; de Haas G H
Influence of size and polarity of residue 31 in porcine pancreatic phospholipase A2 on catalytic properties.
Protein engineering 1990;3(7):599-603.
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1990: Wright G C; Weiss J; Kim K S; Verheij H; Elsbach P
Bacterial phospholipid hydrolysis enhances the destruction of Escherichia coli ingested by rabbit neutrophils. Role of cellular and extracellular phospholipases.
The Journal of clinical investigation 1990;85(6):1925-35.
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1990: Kuipers O P; Dekker N; Verheij H M; de Haas G H
Activities of native and tyrosine-69 mutant phospholipases A2 on phospholipid analogues. A reevaluation of the minimal substrate requirements.
Biochemistry 1990;29(25):6094-102.
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1990: Elsbach P; Weiss J; Wright G; Forst S; van den Bergh C J; Verheij H M
Regulation and role of phospholipases in host-bacteria interaction.
Progress in clinical and biological research 1990;349():1-9.
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1990: Kuipers O P; van den Bergh C J; Verheij H M; de Haas G H
Probing the mechanism of pancreatic phospholipase A2 with the aid of recombinant DNA techniques.
Advances in experimental medicine and biology 1990;279():65-84.
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1989: van Oort M G; Deveer A M; Dijkman R; Tjeenk M L; Verheij H M; de Haas G H; Wenzig E; Götz F
Purification and substrate specificity of Staphylococcus hyicus lipase.
Biochemistry 1989;28(24):9278-85.
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1989: van den Bergh C J; Bekkers A C; Verheij H M; de Haas G H
Glutamic acid 71 and aspartic acid 66 control the binding of the second calcium ion in porcine pancreatic phospholipase A2.
European journal of biochemistry / FEBS 1989;182(2):307-13.
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1989: van den Bergh C J; Slotboom A J; Verheij H M; de Haas G H
The role of Asp-49 and other conserved amino acids in phospholipases A2 and their importance for enzymatic activity.
Journal of cellular biochemistry 1989;39(4):379-90.
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1989: Kuipers O P; Thunnissen M M; de Geus P; Dijkstra B W; Drenth J; Verheij H M; de Haas G H
Enhanced activity and altered specificity of phospholipase A2 by deletion of a surface loop.
Science (New York, N.Y.) 1989;244(4900):82-5.
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1989: Kuipers O P; Dijkman R; Pals C E; Verheij H M; de Haas G H
Evidence for the involvement of tyrosine-69 in the control of stereospecificity of porcine pancreatic phospholipase A2.
Protein engineering 1989;2(6):467-71.
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1989: Horrevoets A J; Hackeng T M; Verheij H M; Dijkman R; de Haas G H
Kinetic characterization of Escherichia coli outer membrane phospholipase A using mixed detergent-lipid micelles.
Biochemistry 1989;28(3):1139-47.
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1988: van den Bergh C J; Slotboom A J; Verheij H M; de Haas G H
The role of aspartic acid-49 in the active site of phospholipase A2. A site-specific mutagenesis study of porcine pancreatic phospholipase A2 and the rationale of the enzymatic activity of [lysine49]phospholipase A2 from Agkistrodon piscivorus piscivorus' venom.
European journal of biochemistry / FEBS 1988;176(2):353-7.
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1987: van den Bergh C J; Bekkers A C; De Geus P; Verheij H M; de Haas G H
Secretion of biologically active porcine prophospholipase A2 by Saccharomyces cerevisiae. Use of the prepro sequence of the alpha-mating factor.
European journal of biochemistry / FEBS 1987;170(1-2):241-6.
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1987: de Geus P; van den Bergh C J; Kuipers O; Verheij H M; Hoekstra W P; de Haas G H
Expression of porcine pancreatic phospholipase A2. Generation of active enzyme by sequence-specific cleavage of a hybrid protein from Escherichia coli.
Nucleic acids research 1987;15(9):3743-59.
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1986: de Geus P; Riegman N H; Horrevoets J G; Verheij H M; de Haas G H
Overproduction and high-yield purification of phospholipase A from the outer membrane of Escherichia coli K-12.
European journal of biochemistry / FEBS 1986;161(1):163-9.
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1984: de Geus P; Verheij H M; Riegman N H; Hoekstra W P; de Haas G H
The pro- and mature forms of the E. coli K-12 outer membrane phospholipase A are identical.
The EMBO journal 1984;3(8):1799-802.
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