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Gert Vriend

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TOP 3
Vincent G H Eijsink; B W Matthews; Gert Vriend
The role of calcium ions in the stability and instability of a thermolysin-like protease.
Daphne H E W Huberts; Hanka Venselaar; Gert Vriend; Marten Veenhuis; Ida J van der Klei
The moonlighting function of pyruvate carboxylase resides in the non-catalytic end of the TIM barrel.
Svein J Horn; Pawel Sikorski; Jannicke B Cederkvist; Gustav Vaaje-Kolstad; Morten Sørlie; Bjørnar Synstad; Gert Vriend; Kjell M Vårum; Vincent G H Eijsink
Costs and benefits of processivity in enzymatic degradation of recalcitrant polysaccharides.

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All Publications

2011: Vincent G H Eijsink; B W Matthews; Gert Vriend
The role of calcium ions in the stability and instability of a thermolysin-like protease.
Protein science : a publication of the Protein Society 2011;20(8):1346-55.
2010: Daphne H E W Huberts; Hanka Venselaar; Gert Vriend; Marten Veenhuis; Ida J van der Klei
The moonlighting function of pyruvate carboxylase resides in the non-catalytic end of the TIM barrel.
Biochimica et biophysica acta 2010;1803(9):1038-42.
2006: Svein J Horn; Pawel Sikorski; Jannicke B Cederkvist; Gustav Vaaje-Kolstad; Morten Sørlie; Bjørnar Synstad; Gert Vriend; Kjell M Vårum; Vincent G H Eijsink
Costs and benefits of processivity in enzymatic degradation of recalcitrant polysaccharides.
Proceedings of the National Academy of Sciences of the United States of America 2006;103(48):18089-94.
2004: Vincent G H Eijsink; Alexandra Bjork-Skaflestad; Sigrid Gåseidnes; Reidun Sirevåg; Bjørnar Synstad; Bertus van den Burg; Gert Vriend
Rational engineering of enzyme stability.
Journal of biotechnology 2004;113(1-3):105-20.
2004: Bjørnar Synstad; Sigrid Gåseidnes; Daan M F van Aalten; Gert Vriend; Jens Nielsen; Vincent G H Eijsink
Mutational and computational analysis of the role of conserved residues in the active site of a family 18 chitinase.
European journal of biochemistry / FEBS 2004;271(2):253-62.
2003: Sigrid Gåseidnes; Bjørnar Synstad; Xiaohong Jia; Hege Kjellesvik; Gert Vriend; Vincent G H Eijsink
Stabilization of a chitinase from Serratia marcescens by Gly-->Ala and Xxx-->Pro mutations.
Protein engineering 2003;16(11):841-6.
2002: Arno de Kreij; Bertus van den Burg; Gerard Venema; Gert Vriend; Vincent G H Eijsink; Jens Nielsen
The effects of modifying the surface charge on the catalytic activity of a thermolysin-like protease.
The Journal of biological chemistry 2002;277(18):15432-8.
2001: Arno de Kreij; Bertus van den Burg; OR Veltman; Gert Vriend; Gerard Venema; Vincent G H Eijsink
The effect of changing the hydrophobic S1' subsite of thermolysin-like proteases on substrate specificity.
European journal of biochemistry / FEBS 2001;268(18):4985-91.
1999: Johanna Mansfeld; Gert Vriend; Bertus van den Burg; Vincent G H Eijsink; Renate Ulbrich-Hofmann
Probing the unfolding region in a thermolysin-like protease by site-specific immobilization.
Biochemistry 1999;38(26):8240-5.
1998: Gert Vriend; H J Berendsen; Bertus van den Burg; Gerard Venema; Vincent G H Eijsink
Early steps in the unfolding of thermolysin-like proteases.
The Journal of biological chemistry 1998;273(52):35074-7.
1998: OR Veltman; Gert Vriend; H J Berendsen; Bertus van den Burg; Gerard Venema; Vincent G H Eijsink
A single calcium binding site is crucial for the calcium-dependent thermal stability of thermolysin-like proteases.
Biochemistry 1998;37(15):5312-9.
1998: OR Veltman; Vincent G H Eijsink; Gert Vriend; Arno de Kreij; Gerard Venema; Bertus van den Burg
Probing catalytic hinge bending motions in thermolysin-like proteases by glycine --> alanine mutations.
Biochemistry 1998;37(15):5305-11.
1998: Bertus van den Burg; Vincent G H Eijsink; Gert Vriend; OR Veltman; Gerard Venema
Rendering one autolysis site in Bacillus subtilis neutral protease resistant to cleavage reveals a new fission.
Biotechnology and applied biochemistry 1998;27 ( Pt 2)():125-32.
1998: Bertus van den Burg; Gert Vriend; OR Veltman; Gerard Venema; Vincent G H Eijsink
Engineering an enzyme to resist boiling.
Proceedings of the National Academy of Sciences of the United States of America 1998;95(5):2056-60.
1997: OR Veltman; Gert Vriend; F Hardy; Johanna Mansfeld; Bertus van den Burg; Gerard Venema; Vincent G H Eijsink
Mutational analysis of a surface area that is critical for the thermal stability of thermolysin-like proteases.
European journal of biochemistry / FEBS 1997;248(2):433-40.
1997: Johanna Mansfeld; Gert Vriend; B W Dijkstra; OR Veltman; Bertus van den Burg; Gerard Venema; Renate Ulbrich-Hofmann; Vincent G H Eijsink
Extreme stabilization of a thermolysin-like protease by an engineered disulfide bond.
The Journal of biological chemistry 1997;272(17):11152-6.
1997: OR Veltman; Gert Vriend; Bertus van den Burg; F Hardy; Gerard Venema; Vincent G H Eijsink
Engineering thermolysin-like proteases whose stability is largely independent of calcium.
FEBS letters 1997;405(2):241-4.
1997: F Frigerio; Immaculada Margarit; Renzo Nogarotto; G Grandi; Gert Vriend; F Hardy; OR Veltman; G Venema; Vincent G H Eijsink
Model building of a thermolysin-like protease by mutagenesis.
Protein engineering 1997;10(3):223-30.
1996: OR Veltman; Gert Vriend; Pieter Middelhoven; Bertus van den Burg; Gerard Venema; Vincent G H Eijsink
Analysis of structural determinants of the stability of thermolysin-like proteases by molecular modelling and site-directed mutagenesis.
Protein engineering 1996;9(12):1181-9.
1995: Daan M F van Aalten; Andrea Amadei; A B Linssen; Vincent G H Eijsink; Gert Vriend; Herman J C Berendsen
The essential dynamics of thermolysin: confirmation of the hinge-bending motion and comparison of simulations in vacuum and water.
Proteins 1995;22(1):45-54.
1995: Vincent G H Eijsink; OR Veltman; W Aukema; Gert Vriend; Gerard Venema
Structural determinants of the stability of thermolysin-like proteinases.
Nature structural biology 1995;2(5):374-9.
1994: Bertus van den Burg; B W Dijkstra; Gert Vriend; B Van der Vinne; Gerard Venema; Vincent G H Eijsink
Protein stabilization by hydrophobic interactions at the surface.
European journal of biochemistry / FEBS 1994;220(3):981-5.
1994: F Hardy; Gert Vriend; B van der Vinne; F Frigerio; G Grandi; Gerard Venema; Vincent G H Eijsink
The effect of engineering surface loops on the thermal stability of Bacillus subtilis neutral protease.
Protein engineering 1994;7(3):425-30.
1993: F Hardy; Gert Vriend; OR Veltman; B van der Vinne; Gerard Venema; Vincent G H Eijsink
Stabilization of Bacillus stearothermophilus neutral protease by introduction of prolines.
FEBS letters 1993;317(1-2):89-92.
1992: Vincent G H Eijsink; Gert Vriend; B van der Vinne; B Hazes; Bertus van den Burg; Gerard Venema
Effects of changing the interaction between subdomains on the thermostability of Bacillus neutral proteases.
Proteins 1992;14(2):224-36.
1992: Vincent G H Eijsink; Gert Vriend; J R Van der Zee; Bertus van den Burg; Gerard Venema
Increasing the thermostability of the neutral proteinase of Bacillus stearothermophilus by improvement of internal hydrogen-bonding.
The Biochemical journal 1992;285 ( Pt 2)():625-8.
1992: Vincent G H Eijsink; B W Dijkstra; Gert Vriend; J R van der Zee; OR Veltman; B van der Vinne; Bertus van den Burg; S Kempe; Gerard Venema
The effect of cavity-filling mutations on the thermostability of Bacillus stearothermophilus neutral protease.
Protein engineering 1992;5(5):421-6.
1992: Vincent G H Eijsink; Gert Vriend; Bertus van den Burg; J R van der Zee; Gerard Venema
Increasing the thermostability of a neutral protease by replacing positively charged amino acids in the N-terminal turn of alpha-helices.
Protein engineering 1992;5(2):165-70.
1992: Vincent G H Eijsink; Gert Vriend; Bertus van den Burg; J R van der Zee; OR Veltman; B K Stulp; Gerard Venema
Introduction of a stabilizing 10 residue beta-hairpin in Bacillus subtilis neutral protease.
Protein engineering 1992;5(2):157-63.
1991: Gert Vriend; H J Berendsen; J R van der Zee; Bertus van den Burg; Gerard Venema; Vincent G H Eijsink
Stabilization of the neutral protease of Bacillus stearothermophilus by removal of a buried water molecule.
Protein engineering 1991;4(8):941-5.
1991: Vincent G H Eijsink; Bertus van den Burg; Gert Vriend; H J Berendsen; Gerard Venema
Thermostability of Bacillus subtilis neutral protease.
Biochemistry international 1991;24(3):517-25.
1991: Vincent G H Eijsink; J R van der Zee; Bertus van den Burg; Gert Vriend; Gerard Venema
Improving the thermostability of the neutral protease of Bacillus stearothermophilus by replacing a buried asparagine by leucine.
FEBS letters 1991;282(1):13-6.
1990: Vincent G H Eijsink; Gert Vriend; Bertus van den Burg; Gerard Venema; B K Stulp
Contribution of the C-terminal amino acid to the stability of Bacillus subtilis neutral protease.
Protein engineering 1990;4(1):99-104.
1990: Gerrit Eggink; H Engel; Gert Vriend; Peter Terpstra; Bernard Witholt
Rubredoxin reductase of Pseudomonas oleovorans. Structural relationship to other flavoprotein oxidoreductases based on one NAD and two FAD fingerprints.
Journal of molecular biology 1990;212(1):135-42.
1989: H A Schreuder; P A Prick; Rik K Wierenga; Gert Vriend; K S Wilson; Wim G J Hol; J Drenth
Crystal structure of the p-hydroxybenzoate hydroxylase-substrate complex refined at 1.9 A resolution. Analysis of the enzyme-substrate and enzyme-product complexes.
Journal of molecular biology 1989;208(4):679-96.
1989: Abraham Schierbeek; M B Swarte; Bauke W Dijkstra; Gert Vriend; Randy J Read; Wim G J Hol; J Drenth; C Betzel
X-ray structure of lipoamide dehydrogenase from Azotobacter vinelandii determined by a combination of molecular and isomorphous replacement techniques.
Journal of molecular biology 1989;206(2):365-79.