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Eugene Vysotski
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Research Profile (preview)
Chemicals & Drugs
Concepts & Ideas
Procedures
Genes & Molecular Sequences
Phenomena
Living Beings
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Network (preview)
17
Lee, John
15
Markova, Svetlana
14
Wang, Bi-Cheng
10
Liu, Zhi-Jie
10
Frank, Ludmila
7
Rose, John
7
Stepanyuk, Galina
6
Deng, Lu
6
Bondar, VS
5
Malikova, Natalia
3
Rose, John
3
Alakhov, Yuli
3
Blinks, John
3
Golz, Stefan
2
van Berkel, Willem
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Geonetwork of Eugene Vysotski (preview)
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All Publications
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2009: van Oort Bart; Eremeeva Elena V; Koehorst Rob B M; Laptenok Sergey P; van Amerongen Herbert; van Berkel Willem J H; Malikova Natalia P; Markova Svetlana V; Vysotski Eugene S; Visser Antonie J W G; Lee John
Picosecond fluorescence relaxation spectroscopy of the calcium-discharged photoproteins aequorin and obelin.
Biochemistry 2009;48(44):10486-91.
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2009: Eremeeva Elena V; Markova Svetlana V; Westphal Adrie H; Visser Antonie J W G; van Berkel Willem J H; Vysotski Eugene S
The intrinsic fluorescence of apo-obelin and apo-aequorin and use of its quenching to characterize coelenterazine binding.
FEBS letters 2009;583(12):1939-44.
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2009: Stepanyuk Galina A; Liu Zhi-Jie; Vysotski Eugene S; Lee John; Rose John P; Wang Bi-Cheng
Structure based mechanism of the Ca(2+)-induced release of coelenterazine from the Renilla binding protein.
Proteins 2009;74(3):583-93.
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2008: Stepanyuk Galina A; Xu Hao; Wu Chia-Kuei; Markova Svetlana V; Lee John; Vysotski Eugene S; Wang Bi-Cheng
Expression, purification and characterization of the secreted luciferase of the copepod Metridia longa from Sf9 insect cells.
Protein expression and purification 2008;61(2):142-8.
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2008: Borisova Vasilisa V; Frank Ludmila A; Markova Svetlana V; Burakova Ludmila P; Vysotski Eugene S
Recombinant Metridia luciferase isoforms: expression, refolding and applicability for in vitro assay.
Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology 2008;7(9):1025-31.
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2008: Frank Ludmila A; Borisova Vasilisa V; Markova Svetlana V; Malikova Natalia P; Stepanyuk Galina A; Vysotski Eugene S
Violet and greenish photoprotein obelin mutants for reporter applications in dual-color assay.
Analytical and bioanalytical chemistry 2008;391(8):2891-6.
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2008: Stepanyuk Galina A; Liu Zhi-Jie; Markova Svetlana S; Frank Ludmila A; Lee John; Vysotski Eugene S; Wang Bi-Cheng
Crystal structure of coelenterazine-binding protein from Renilla muelleri at 1.7 A: why it is not a calcium-regulated photoprotein.
Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology 2008;7(4):442-7.
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2008: Titushin Maxim S; Markova Svetlana V; Frank Ludmila A; Malikova Natalia P; Stepanyuk Galina A; Lee John; Vysotski Eugene S
Coelenterazine-binding protein of Renilla muelleri: cDNA cloning, overexpression, and characterization as a substrate of luciferase.
Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology 2008;7(2):189-96.
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2007: Frank L; Markova S; Remmel N; Vysotski E; Gitelson I
Bioluminescent signal system: bioluminescence immunoassay of pathogenic organisms.
Luminescence : the journal of biological and chemical luminescence 2007;22(3):215-20.
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2006: Lamarcq Laurence H; Scherer Bradley J; Phelan Michael L; Kalnine Nikolai N; Nguyen Yen H; Kabakova Tatyana; Chen Xiaoyi; Tan Marcia; Chang Cynthia; Berlon Charina; Campos-Gonzalez Roberto; Gao Guo-Jian; Golz Stefan; Vysotski Eugene S; Farmer Andrew A
Large-scale, high-throughput validation of short hairpin RNA sequences for RNA interference.
Journal of biomolecular screening : the official journal of the Society for Biomolecular Screening 2006;11(3):236-46.
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2006: Liu Zhi-Jie; Stepanyuk Galina A; Vysotski Eugene S; Lee John; Markova Svetlana V; Malikova Natalia P; Wang Bi-Cheng
Crystal structure of obelin after Ca2+-triggered bioluminescence suggests neutral coelenteramide as the primary excited state.
Proceedings of the National Academy of Sciences of the United States of America 2006;103(8):2570-5.
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2005: Deng Lu; Vysotski Eugene S; Markova Svetlana V; Liu Zhi-Jie; Lee John; Rose John; Wang Bi-Cheng
All three Ca2+-binding loops of photoproteins bind calcium ions: the crystal structures of calcium-loaded apo-aequorin and apo-obelin.
Protein science : a publication of the Protein Society 2005;14(3):663-75.
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2005: Stepanyuk Galina A; Golz Stefan; Markova Svetlana V; Frank Ludmila A; Lee John; Vysotski Eugene S
Interchange of aequorin and obelin bioluminescence color is determined by substitution of one active site residue of each photoprotein.
FEBS letters 2005;579(5):1008-14.
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2004: Deng Lu; Markova Svetlana V; Vysotski Eugene S; Liu Zhi-Jie; Lee John; Rose John; Wang Bi-Cheng
Crystal structure of a Ca2+-discharged photoprotein: implications for mechanisms of the calcium trigger and bioluminescence.
The Journal of biological chemistry 2004;279(32):33647-52.
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2004: Vysotski Eugene S; Lee John
Ca2+-regulated photoproteins: structural insight into the bioluminescence mechanism.
Accounts of chemical research 2004;37(6):405-15.
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2004: Deng Lu; Markova Svetlana V; Vysotski Eugene S; Liu Zhi-Jie; Lee John; Rose John; Wang Bi-Cheng
Preparation and X-ray crystallographic analysis of the Ca2+-discharged photoprotein obelin.
Acta crystallographica. Section D, Biological crystallography 2004;60(Pt 3):512-4.
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2004: Frank Ludmila A; Petunin Aleksei I; Vysotski Eugene S
Bioluminescent immunoassay of thyrotropin and thyroxine using obelin as a label.
Analytical biochemistry 2004;325(2):240-6.
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2004: Markova Svetlana V; Golz Stefan; Frank Ludmila A; Kalthof Bernd; Vysotski Eugene S
Cloning and expression of cDNA for a luciferase from the marine copepod Metridia longa. A novel secreted bioluminescent reporter enzyme.
The Journal of biological chemistry 2004;279(5):3212-7.
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2003: Malikova Natalia P; Stepanyuk Galina A; Frank Ludmila A; Markova Svetlana V; Vysotski Eugene S; Lee John
Spectral tuning of obelin bioluminescence by mutations of Trp92.
FEBS letters 2003;554(1-2):184-8.
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2003: Liu Zhi-Jie; Vysotski Eugene S; Deng Lu; Lee John; Rose John; Wang Bi-Cheng
Atomic resolution structure of obelin: soaking with calcium enhances electron density of the second oxygen atom substituted at the C2-position of coelenterazine.
Biochemical and biophysical research communications 2003;311(2):433-9.
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2003: Vysotski Eugene S; Liu Zhi-Jie; Markova Svetlana V; Blinks John R; Deng Lu; Frank Ludmila A; Herko Michelle; Malikova Natalia P; Rose John P; Wang Bi-Cheng; Lee John
Violet bioluminescence and fast kinetics from W92F obelin: structure-based proposals for the bioluminescence triggering and the identification of the emitting species.
Biochemistry 2003;42(20):6013-24.
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2002: Markova Svetlana V; Vysotski Eugene S; Blinks John R; Burakova Ludmila P; Wang B-C; Lee John
Obelin from the bioluminescent marine hydroid Obelia geniculata: cloning, expression, and comparison of some properties with those of other Ca2+-regulated photoproteins.
Biochemistry 2002;41(7):2227-36.
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2001: Vysotski E S; Liu Z J; Rose J; Wang B C; Lee J
Preparation and X-ray crystallographic analysis of recombinant obelin crystals diffracting to beyond 1.1 A.
Acta crystallographica. Section D, Biological crystallography 2001;57(Pt 12):1919-21.
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2001: Deng L; Vysotski E S; Liu Z J; Markova S V; Malikova N P; Lee J; Rose J; Wang B C
Structural basis for the emission of violet bioluminescence from a W92F obelin mutant.
FEBS letters 2001;506(3):281-5.
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2000: Liu Z J; Vysotski E S; Chen C J; Rose J P; Lee J; Wang B C
Structure of the Ca2+-regulated photoprotein obelin at 1.7 A resolution determined directly from its sulfur substructure.
Protein science : a publication of the Protein Society 2000;9(11):2085-93.
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2000: Illarionov B A; Frank L A; Illarionova V A; Bondar V S; Vysotski E S; Blinks J R
Recombinant obelin: cloning and expression of cDNA purification, and characterization as a calcium indicator.
Methods in enzymology 2000;305():223-49.
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1999: Vysotski E S; Liu Z J; Rose J; Wang B C; Lee J
Preparation and preliminary study of crystals of the recombinant calcium-regulated photoprotein obelin from the bioluminescent hydroid Obelia longissima.
Acta crystallographica. Section D, Biological crystallography 1999;55(Pt 11):1965-6.
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1999: Berestovskaya N G; Shaloiko L A; Gorokhovatsky A Y; Bondar V S; Vysotski E S; Maximov J E; von Doehren H; Alakhov Y B
Cotranslational formation of active photoprotein obelin in a cell-free translation system: direct ultrahigh sensitive measure of the translation course.
Analytical biochemistry 1999;268(1):72-8.
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1998: Gorokhovatsky AYu; Shaloiko L A; Bondar V S; Vysotski E S; Maximov E E; von Doehren H; Alakhov Y B
Cell-free bioluminescent screening of translation inhibitors.
Biotechnology and applied biochemistry 1998;27 ( Pt 3)():259-63.
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1996: Frank L A; Illarionova V A; Vysotski E S
Use of proZZ-obelin fusion protein in bioluminescent immunoassay.
Biochemical and biophysical research communications 1996;219(2):475-9.
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1995: Matveev S V; Illarionov B A; Vysotski E S; Bondar V S; Markova S V; Alakhov Y B
Obelin mRNA--a new tool for studies of translation in cell-free systems.
Analytical biochemistry 1995;231(1):34-9.
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1995: Illarionov B A; Bondar V S; Illarionova V A; Vysotski E S
Sequence of the cDNA encoding the Ca(2+)-activated photoprotein obelin from the hydroid polyp Obelia longissima.
Gene 1995;153(2):273-4.
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1995: Vysotski E S; Trofimov C P; Bondar V S; Frank L A; Markova S V; Illarionov B A
Mn(2+)-activated luminescence of the photoprotein obelin.
Archives of biochemistry and biophysics 1995;316(1):92-9.
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