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Takayoshi Wakagi
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49
Fushinobu, Shinya
41
Shoun, Hirofumi
23
Matsuzawa, Hiroshi
11
Kitaoka, Motomitsu
8
Hidaka, Masafumi
8
Imamura, Hiromi
7
Fukuda, Eriko
7
Miyanaga, Akimasa
7
Nojiri, Hideaki
7
Omori, Toshio
5
Yamane, Hisakazu
5
Jeon, Beong-Sam
4
Jeong, Jong-Jin
4
Jun, So-Young
4
Ito, Sohei
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All Publications
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2009: Manabe Fumitoshi; Itoh Yuko H; Shoun Hirofumi; Wakagi Takayoshi
Membrane-bound acid pyrophosphatase from Sulfolobus tokodaii, a thermoacidophilic archaeon: heterologous expression of the gene and characterization of the product.
Extremophiles : life under extreme conditions 2009;13(6):859-65.
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2009: Suzuki Ryuichiro; Katayama Takane; Kitaoka Motomitsu; Kumagai Hidehiko; Wakagi Takayoshi; Shoun Hirofumi; Ashida Hisashi; Yamamoto Kenji; Fushinobu Shinya
Crystallographic and mutational analyses of substrate recognition of endo-alpha-N-acetylgalactosaminidase from Bifidobacterium longum.
Journal of biochemistry 2009;146(3):389-98.
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2009: Kim Sang-Wan; Fushinobu Shinya; Zhou Shengmin; Wakagi Takayoshi; Shoun Hirofumi
Eukaryotic nirK genes encoding copper-containing nitrite reductase: originating from the protomitochondrion?
Applied and environmental microbiology 2009;75(9):2652-8.
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2009: Zhou Shengmin; Fushinobu Shinya; Nakanishi Yoshito; Kim Sang-Wan; Wakagi Takayoshi; Shoun Hirofumi
Cloning and characterization of two flavohemoglobins from Aspergillus oryzae.
Biochemical and biophysical research communications 2009;381(1):7-11.
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2009: Hidaka Masafumi; Nishimoto Mamoru; Kitaoka Motomitsu; Wakagi Takayoshi; Shoun Hirofumi; Fushinobu Shinya
The crystal structure of galacto-N-biose/lacto-N-biose I phosphorylase: a large deformation of a TIM barrel scaffold.
The Journal of biological chemistry 2009;284(11):7273-83.
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2009: Xu Lian-Hua; Fushinobu Shinya; Ikeda Haruo; Wakagi Takayoshi; Shoun Hirofumi
Crystal structures of cytochrome P450 105P1 from Streptomyces avermitilis: conformational flexibility and histidine ligation state.
Journal of bacteriology 2009;191(4):1211-9.
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2009: Luo Jing; Fukuda Eriko; Takase Hirofumi; Fushinobu Shinya; Shoun Hirofumi; Wakagi Takayoshi
Identification of the lysine residue responsible for coenzyme A binding in the heterodimeric 2-oxoacid:ferredoxin oxidoreductase from Sulfolobus tokodaii, a thermoacidophilic archaeon, using 4-fluoro-7-nitrobenzofurazan as an affinity label.
Biochimica et biophysica acta 2009;1794(2):335-40.
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2008: Suzuki Ryuichiro; Wada Jun; Katayama Takane; Fushinobu Shinya; Wakagi Takayoshi; Shoun Hirofumi; Sugimoto Hayuki; Tanaka Akiyoshi; Kumagai Hidehiko; Ashida Hisashi; Kitaoka Motomitsu; Yamamoto Kenji
Structural and thermodynamic analyses of solute-binding Protein from Bifidobacterium longum specific for core 1 disaccharide and lacto-N-biose I.
The Journal of biological chemistry 2008;283(19):13165-73.
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2008: Honda Yuji; Fushinobu Shinya; Hidaka Masafumi; Wakagi Takayoshi; Shoun Hirofumi; Taniguchi Hajime; Kitaoka Motomitsu
Alternative strategy for converting an inverting glycoside hydrolase into a glycosynthase.
Glycobiology 2008;18(4):325-30.
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2008: Takaki Koki; Fushinobu Shinya; Kim Sang-Wan; Miyahara Morio; Wakagi Takayoshi; Shoun Hirofumi
Streptomyces griseus enhances denitrification by Ralstonia pickettii K50, which is possibly mediated by histidine produced during co-culture.
Bioscience, biotechnology, and biochemistry 2008;72(1):163-70.
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2007: Wada Jun; Suzuki Ryuichiro; Fushinobu Shinya; Kitaoka Motomitsu; Wakagi Takayoshi; Shoun Hirofumi; Ashida Hisashi; Kumagai Hidehiko; Katayama Takane; Yamamoto Kenji
Purification, crystallization and preliminary X-ray analysis of the galacto-N-biose-/lacto-N-biose I-binding protein (GL-BP) of the ABC transporter from Bifidobacterium longum JCM1217.
Acta crystallographica. Section F, Structural biology and crystallization communications 2007;63(Pt 9):751-3.
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2007: Nijikken Yuri; Tsukada Takeshi; Igarashi Kiyohiko; Samejima Masahiro; Wakagi Takayoshi; Shoun Hirofumi; Fushinobu Shinya
Crystal structure of intracellular family 1 beta-glucosidase BGL1A from the basidiomycete Phanerochaete chrysosporium.
FEBS letters 2007;581(7):1514-20.
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2007: Nishimasu Hiroshi; Fushinobu Shinya; Shoun Hirofumi; Wakagi Takayoshi
Crystal structures of an ATP-dependent hexokinase with broad substrate specificity from the hyperthermophilic archaeon Sulfolobus tokodaii.
The Journal of biological chemistry 2007;282(13):9923-31.
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2007: Kakugawa Satoshi; Fushinobu Shinya; Wakagi Takayoshi; Shoun Hirofumi
Characterization of a thermostable carboxylesterase from the hyperthermophilic bacterium Thermotoga maritima.
Applied microbiology and biotechnology 2007;74(3):585-91.
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2006: Miyanaga Akimasa; Koseki Takuya; Miwa Yozo; Mese Yuichiro; Nakamura Sachiko; Kuno Atsushi; Hirabayashi Jun; Matsuzawa Hiroshi; Wakagi Takayoshi; Shoun Hirofumi; Fushinobu Shinya
The family 42 carbohydrate-binding module of family 54 alpha-L-arabinofuranosidase specifically binds the arabinofuranose side chain of hemicellulose.
The Biochemical journal 2006;399(3):503-11.
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2006: Koseki Takuya; Miwa Yozo; Mese Yuichiro; Miyanaga Akimasa; Fushinobu Shinya; Wakagi Takayoshi; Shoun Hirofumi; Matsuzawa Hiroshi; Hashizume Katsumi
Mutational analysis of N-glycosylation recognition sites on the biochemical properties of Aspergillus kawachii alpha-L-arabinofuranosidase 54.
Biochimica et biophysica acta 2006;1760(9):1458-64.
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2006: Hidaka Masafumi; Kitaoka Motomitsu; Hayashi Kiyoshi; Wakagi Takayoshi; Shoun Hirofumi; Fushinobu Shinya
Structural dissection of the reaction mechanism of cellobiose phosphorylase.
The Biochemical journal 2006;398(1):37-43.
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2006: Jun So-Young; Fushinobu Shinya; Nojiri Hideaki; Omori Toshio; Shoun Hirofumi; Wakagi Takayoshi
Improving the catalytic efficiency of a meta-cleavage product hydrolase (CumD) from Pseudomonas fluorescens IP01.
Biochimica et biophysica acta 2006;1764(7):1159-66.
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2006: Nishimasu Hiroshi; Fushinobu Shinya; Shoun Hirofumi; Wakagi Takayoshi
Identification and characterization of an ATP-dependent hexokinase with broad substrate specificity from the hyperthermophilic archaeon Sulfolobus tokodaii.
Journal of bacteriology 2006;188(5):2014-9.
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2006: Nakajima Masahiro; Fushinobu Shinya; Imamura Hiromi; Shoun Hirofumi; Wakagi Takayoshi
Crystallization and preliminary X-ray analysis of cytosolic alpha-mannosidase from Thermotoga maritima.
Acta crystallographica. Section F, Structural biology and crystallization communications 2006;62(Pt 2):104-5.
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2005: Yamada Tomoya; Fujii Takeshi; Kanai Tamotsu; Amo Taku; Imanaka Tadayuki; Nishimasu Hiroshi; Wakagi Takayoshi; Shoun Hirofumi; Kamekura Masahiro; Kamagata Yoichi; Kato Takeshi; Kawashima Koichiro
Expression of acetylcholine (ACh) and ACh-synthesizing activity in Archaea.
Life sciences 2005;77(16):1935-44.
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2005: Nunoura Takuro; Sako Yoshihiko; Wakagi Takayoshi; Uchida Aritsune
Cytochrome aa3 in facultatively aerobic and hyperthermophilic archaeon Pyrobaculum oguniense.
Canadian journal of microbiology 2005;51(8):621-7.
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2005: Nishizawa Yumie; Yabuki Takashi; Fukuda Eriko; Wakagi Takayoshi
Gene expression and characterization of two 2-oxoacid:ferredoxin oxidoreductases from Aeropyrum pernix K1.
FEBS letters 2005;579(11):2319-22.
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2005: Dong Xuesong; Fushinobu Shinya; Fukuda Eriko; Terada Tohru; Nakamura Shugo; Shimizu Kentaro; Nojiri Hideaki; Omori Toshio; Shoun Hirofumi; Wakagi Takayoshi
Crystal structure of the terminal oxygenase component of cumene dioxygenase from Pseudomonas fluorescens IP01.
Journal of bacteriology 2005;187(7):2483-90.
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2005: Fushinobu Shinya; Hidaka Masafumi; Honda Yuji; Wakagi Takayoshi; Shoun Hirofumi; Kitaoka Motomitsu
Structural basis for the specificity of the reducing end xylose-releasing exo-oligoxylanase from Bacillus halodurans C-125.
The Journal of biological chemistry 2005;280(17):17180-6.
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2005: Honda Yuji; Fushinobu Shinya; Hidaka Masafumi; Wakagi Takayoshi; Shoun Hirofumi; Kitaoka Motomitsu
Crystallization and preliminary X-ray analysis of reducing-end xylose-releasing exo-oligoxylanase from Bacillus halodurans C-125.
Acta crystallographica. Section F, Structural biology and crystallization communications 2005;61(Pt 3):291-2.
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2005: Fushinobu Shinya; Jun So-Young; Hidaka Masafumi; Nojiri Hideaki; Yamane Hisakazu; Shoun Hirofumi; Omori Toshio; Wakagi Takayoshi
A series of crystal structures of a meta-cleavage product hydrolase from Pseudomonas fluorescens IP01 (CumD) complexed with various cleavage products.
Bioscience, biotechnology, and biochemistry 2005;69(3):491-8.
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2005: Nam Jeong-Won; Noguchi Haruko; Fujimoto Zui; Mizuno Hiroshi; Ashikawa Yuji; Abo Mitsuru; Fushinobu Shinya; Kobashi Nobuyuki; Wakagi Takayoshi; Iwata Kenichi; Yoshida Takako; Habe Hiroshi; Yamane Hisakazu; Omori Toshio; Nojiri Hideaki
Crystal structure of the ferredoxin component of carbazole 1,9a-dioxygenase of Pseudomonas resinovorans strain CA10, a novel Rieske non-heme iron oxygenase system.
Proteins 2005;58(4):779-89.
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2004: Nakajima Masahiro; Imamura Hiromi; Shoun Hirofumi; Horinouchi Sueharu; Wakagi Takayoshi
Transglycosylation activity of Dictyoglomus thermophilum amylase A.
Bioscience, biotechnology, and biochemistry 2004;68(11):2369-73.
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2004: Hidaka Masafumi; Kitaoka Motomitsu; Hayashi Kiyoshi; Wakagi Takayoshi; Shoun Hirofumi; Fushinobu Shinya
Crystallization and preliminary X-ray analysis of cellobiose phosphorylase from Cellvibrio gilvus.
Acta crystallographica. Section D, Biological crystallography 2004;60(Pt 10):1877-8.
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2004: Miyanaga Akimasa; Koseki Takuya; Matsuzawa Hiroshi; Wakagi Takayoshi; Shoun Hirofumi; Fushinobu Shinya
Crystal structure of a family 54 alpha-L-arabinofuranosidase reveals a novel carbohydrate-binding module that can bind arabinose.
The Journal of biological chemistry 2004;279(43):44907-14.
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2004: Miyanaga Akimasa; Koseki Takuya; Matsuzawa Hiroshi; Wakagi Takayoshi; Shoun Hirofumi; Fushinobu Shinya
Expression, purification, crystallization and preliminary X-ray analysis of alpha-L-arabinofuranosidase B from Aspergillus kawachii.
Acta crystallographica. Section D, Biological crystallography 2004;60(Pt 7):1286-8.
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2004: Nishimasu Hiroshi; Fushinobu Shinya; Shoun Hirofumi; Wakagi Takayoshi
The first crystal structure of the novel class of fructose-1,6-bisphosphatase present in thermophilic archaea.
Structure (London, England : 1993) 2004;12(6):949-59.
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2004: Hidaka Masafumi; Honda Yuji; Kitaoka Motomitsu; Nirasawa Satoru; Hayashi Kiyoshi; Wakagi Takayoshi; Shoun Hirofumi; Fushinobu Shinya
Chitobiose phosphorylase from Vibrio proteolyticus, a member of glycosyl transferase family 36, has a clan GH-L-like (alpha/alpha)(6) barrel fold.
Structure (London, England : 1993) 2004;12(6):937-47.
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2004: Imamura Hiromi; Jeon Beon-Sam; Wakagi Takayoshi
Molecular evolution of the ATPase subunit of three archaeal sugar ABC transporters.
Biochemical and biophysical research communications 2004;319(1):230-4.
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2004: Jeong Jong-Jin; Sonoda Tomoaki; Fushinobu Shinya; Shoun Hirofumi; Wakagi Takayoshi
Crystal structure of isocitrate dehydrogenase from Aeropyrum pernix.
Proteins 2004;55(4):1087-9.
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2004: Nishimoto Mamoru; Fushinobu Shinya; Miyanaga Akimasa; Wakagi Takayoshi; Shoun Hirofumi; Sakka Kazuo; Ohmiya Kunio; Nirasawa Satoru; Kitaoka Motomitsu; Hayashi Kiyoshi
Crystallization and preliminary X-ray analysis of xylanase B from Clostridium stercorarium.
Acta crystallographica. Section D, Biological crystallography 2004;60(Pt 2):342-3.
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2004: Miyanaga Akimasa; Fushinobu Shinya; Ito Kiyoshi; Shoun Hirofumi; Wakagi Takayoshi
Mutational and structural analysis of cobalt-containing nitrile hydratase on substrate and metal binding.
European journal of biochemistry / FEBS 2004;271(2):429-38.
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2003: Fushinobu Shinya; Shoun Hirofumi; Wakagi Takayoshi
Crystal structure of sulerythrin, a rubrerythrin-like protein from a strictly aerobic archaeon, Sulfolobus tokodaii strain 7, shows unexpected domain swapping.
Biochemistry 2003;42(40):11707-15.
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2003: Ito Sohei; Fushinobu Shinya; Jeong Jong-Jin; Yoshioka Issei; Koga Shinji; Shoun Hirofumi; Wakagi Takayoshi
Crystal structure of an ADP-dependent glucokinase from Pyrococcus furiosus: implications for a sugar-induced conformational change in ADP-dependent kinase.
Journal of molecular biology 2003;331(4):871-83.
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2003: Jeong Jong-Jin; Fushinobu Shinya; Ito Sohei; Shoun Hirofumi; Wakagi Takayoshi
Archaeal ADP-dependent phosphofructokinase: expression, purification, crystallization and preliminary crystallographic analysis.
Acta crystallographica. Section D, Biological crystallography 2003;59(Pt 7):1327-9.
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2003: Nakajima Masahiro; Imamura Hiromi; Shoun Hirofumi; Wakagi Takayoshi
Unique metal dependency of cytosolic alpha-mannosidase from Thermotoga maritima, a hyperthermophilic bacterium.
Archives of biochemistry and biophysics 2003;415(1):87-93.
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2003: Wakagi Takayoshi
Sulerythrin, the smallest member of the rubrerythrin family, from a strictly aerobic and thermoacidophilic archaeon, Sulfolobus tokodaii strain 7.
FEMS microbiology letters 2003;222(1):33-7.
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2003: Imamura Hiromi; Fushinobu Shinya; Yamamoto Masaki; Kumasaka Takashi; Jeon Beong-Sam; Wakagi Takayoshi; Matsuzawa Hiroshi
Crystal structures of 4-alpha-glucanotransferase from Thermococcus litoralis and its complex with an inhibitor.
The Journal of biological chemistry 2003;278(21):19378-86.
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2003: Habe Hiroshi; Morii Kenichi; Fushinobu Shinya; Nam Jeong-Won; Ayabe Yuko; Yoshida Takako; Wakagi Takayoshi; Yamane Hisakazu; Nojiri Hideaki; Omori Toshio
Crystal structure of a histidine-tagged serine hydrolase involved in the carbazole degradation (CarC enzyme).
Biochemical and biophysical research communications 2003;303(2):631-9.
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2003: Nunoura Takuro; Sako Yoshihiko; Wakagi Takayoshi; Uchida Aritsune
Regulation of the aerobic respiratory chain in the facultatively aerobic and hyperthermophilic archaeon Pyrobaculum oguniense.
Microbiology (Reading, England) 2003;149(Pt 3):673-88.
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2003: Jeong Jong-Jin; Fushinobu Shinya; Ito Sohei; Jeon Beong-Sam; Shoun Hirofumi; Wakagi Takayoshi
Characterization of the cupin-type phosphoglucose isomerase from the hyperthermophilic archaeon Thermococcus litoralis.
FEBS letters 2003;535(1-3):200-4.
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2002: Ishikawa Ryuhei; Ishido Yoko; Tachikawa Atsuo; Kawasaki Hiroshi; Matsuzawa Hiroshi; Wakagi Takayoshi
Aeropyrum pernix K1, a strictly aerobic and hyperthermophilic archaeon, has two terminal oxidases, cytochrome ba3 and cytochrome aa3.
Archives of microbiology 2002;179(1):42-9.
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2002: Shimamura Tatsuro; Ibuka Akiko; Fushinobu Shinya; Wakagi Takayoshi; Ishiguro Masaji; Ishii Yoshikazu; Matsuzawa Hiroshi
Acyl-intermediate structures of the extended-spectrum class A beta-lactamase, Toho-1, in complex with cefotaxime, cephalothin, and benzylpenicillin.
The Journal of biological chemistry 2002;277(48):46601-8.
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2002: Puchkaev Andrei V; Wakagi Takayoshi; Ortiz de Montellano Paul R
CYP119 plus a Sulfolobus tokodaii strain 7 ferredoxin and 2-oxoacid:ferredoxin oxidoreductase constitute a high-temperature cytochrome P450 catalytic system.
Journal of the American Chemical Society 2002;124(43):12682-3.
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2002: Hidaka Masafumi; Fushinobu Shinya; Ohtsu Naomi; Motoshima Hidemasa; Matsuzawa Hiroshi; Shoun Hirofumi; Wakagi Takayoshi
Trimeric crystal structure of the glycoside hydrolase family 42 beta-galactosidase from Thermus thermophilus A4 and the structure of its complex with galactose.
Journal of molecular biology 2002;322(1):79-91.
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2002: Fushinobu Shinya; Saku Takashi; Hidaka Masafumi; Jun So-Young; Nojiri Hideaki; Yamane Hisakazu; Shoun Hirofumi; Omori Toshio; Wakagi Takayoshi
Crystal structures of a meta-cleavage product hydrolase from Pseudomonas fluorescens IP01 (CumD) complexed with cleavage products.
Protein science : a publication of the Protein Society 2002;11(9):2184-95.
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2002: Suefuji Kyoko; Lin Shie-Jea; Wakagi Takayoshi; Matsuzawa Hiroshi; Yoshimura Etsuro
Sodium-23 and lanthanum-139 nuclear magnetic resonance studies of cation binding to aqualysin I, a thermostable serine protease.
Bioscience, biotechnology, and biochemistry 2002;66(6):1281-6.
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2002: Fukuda Eriko; Wakagi Takayoshi
Substrate recognition by 2-oxoacid:ferredoxin oxidoreductase from Sulfolobus sp. strain 7.
Biochimica et biophysica acta 2002;1597(1):74-80.
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2002: Murata Takashi; Fushinobu Shinya; Nakajima Masatoshi; Asami Osamu; Sassa Takeshi; Wakagi Takayoshi; Yamaguchi Isomaro
Crystal structure of the liganded anti-gibberellin A(4) antibody 4-B8(8)/E9 Fab fragment.
Biochemical and biophysical research communications 2002;293(1):489-96.
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2002: Kojoh Kanehisa; Fukuda Eriko; Matsuzawa Hiroshi; Wakagi Takayoshi
Zinc-coordination of aspartic acid-76 in Sulfolobus ferredoxin is not required for thermal stability of the molecule.
Journal of inorganic biochemistry 2002;89(1-2):69-73.
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2002: Uchikoba Hiroyuki; Fushinobu Shinya; Wakagi Takayoshi; Konno Michiko; Taguchi Hayao; Matsuzawa Hiroshi
Crystal structure of non-allosteric L-lactate dehydrogenase from Lactobacillus pentosus at 2.3 A resolution: specific interactions at subunit interfaces.
Proteins 2002;46(2):206-14.
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2002: Wakagi Takayoshi; Fukuda Eriko; Ogawa Yoko; Kino Hiroyasu; Matsuzawa Hiroshi
A novel bifunctional molybdo-enzyme catalyzing both decarboxylation of indolepyruvate and oxidation of indoleacetaldehyde from a thermoacidophilic archaeon, Sulfolobus sp. strain 7.
FEBS letters 2002;510(3):196-200.
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2002: Saku Takashi; Fushinobu Shinya; Jun So-Young; Ikeda Naohiro; Nojiri Hideaki; Yamane Hisakazu; Omori Toshio; Wakagi Takayoshi
Purification, characterization, and steady-state kinetics of a meta-cleavage compound hydrolase from Pseudomonas fluorescens IPO1.
Journal of bioscience and bioengineering 2002;93(6):568-74.
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2002: Nagaoka Yoshiyuki; Yokozawa Junji; Umehara Takuya; Iwaki Jun; Okamoto Koji; Kawarabayasi Yutaka; Koyama Yoshinori; Sako Yoshihiko; Wakagi Takayoshi; Kuno Atsushi; Hasegawa Tsunemi
Molecular recognition of threonine tRNA by threonyl-tRNA synthetase from an extreme thermophilic archaeon, Aeropyrum pernix K1.
Nucleic acids research. Supplement (2001) 2002;(2):81-2.
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2001: Miyanaga A; Fushinobu S; Ito K; Wakagi T
Crystal structure of cobalt-containing nitrile hydratase.
Biochemical and biophysical research communications 2001;288(5):1169-74.
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2001: Fukuda E; Kino H; Matsuzawa H; Wakagi T
Role of a highly conserved YPITP motif in 2-oxoacid:ferredoxin oxidoreductase: heterologous expression of the gene from Sulfolobus sp.strain 7, and characterization of the recombinant and variant enzymes.
European journal of biochemistry / FEBS 2001;268(21):5639-46.
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2001: Imamura H; Fushinobu S; Jeon B S; Wakagi T; Matsuzawa H
Identification of the catalytic residue of Thermococcus litoralis 4-alpha-glucanotransferase through mechanism-based labeling.
Biochemistry 2001;40(41):12400-6.
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2001: Ito S; Fushinobu S; Yoshioka I; Koga S; Matsuzawa H; Wakagi T
Structural basis for the ADP-specificity of a novel glucokinase from a hyperthermophilic archaeon.
Structure (London, England : 1993) 2001;9(3):205-14.
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2001: Yokozawa J; Nagaoka Y; Umehara T; Iwaki J; Kawarabayasi Y; Koyama Y; Sako Y; Wakagi T; Kuno A; Hasegawa T
Recognition of tRNA by aminoacyl-tRNA synthetase from hyperthermophilic archaea, Aeropyrum pernix K1.
Nucleic acids research. Supplement (2001) 2001;(1):117-8.
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1999: Makino S; Makino T; Abe K; Hashimoto J; Tatsuta T; Kitagawa M; Mori H; Ogura T; Fujii T; Fushinobu S; Wakagi T; Matsuzawa H;
Second transmembrane segment of FtsH plays a role in its proteolytic activity and homo-oligomerization.
FEBS letters 1999;460(3):554-8.
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1999: Imamura H; Jeon B; Wakagi T; Matsuzawa H
High level expression of Thermococcus litoralis 4-alpha-glucanotransferase in a soluble form in Escherichia coli with a novel expression system involving minor arginine tRNAs and GroELS.
FEBS letters 1999;457(3):393-6.
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1999: Kojoh K; Matsuzawa H; Wakagi T
Zinc and an N-terminal extra stretch of the ferredoxin from a thermoacidophilic archaeon stabilize the molecule at high temperature.
European journal of biochemistry / FEBS 1999;264(1):85-91.
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1999: Lin S J; Yoshimura E; Sakai H; Wakagi T; Matsuzawa H
Weakly bound calcium ions involved in the thermostability of aqualysin I, a heat-stable subtilisin-type protease of Thermus aquaticus YT-1.
Biochimica et biophysica acta 1999;1433(1-2):132-8.
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1999: Wakagi T
[Recent progress on the study of structural zinc]
Seikagaku. The Journal of Japanese Biochemical Society 1999;71(1):69-74.
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1998: Wakagi T; Oshima T; Imamura H; Matsuzawa H
Cloning of the gene for inorganic pyrophosphatase from a thermoacidophilic archaeon, Sulfolobus sp. strain 7, and overproduction of the enzyme by coexpression of tRNA for arginine rare codon.
Bioscience, biotechnology, and biochemistry 1998;62(12):2408-14.
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1998: Fushinobu S; Ito K; Konno M; Wakagi T; Matsuzawa H
Crystallographic and mutational analyses of an extremely acidophilic and acid-stable xylanase: biased distribution of acidic residues and importance of Asp37 for catalysis at low pH.
Protein engineering 1998;11(12):1121-8.
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1997: Jeon B S; Taguchi H; Sakai H; Ohshima T; Wakagi T; Matsuzawa H
4-alpha-glucanotransferase from the hyperthermophilic archaeon Thermococcus litoralis--enzyme purification and characterization, and gene cloning, sequencing and expression in Escherichia coli.
European journal of biochemistry / FEBS 1997;248(1):171-8.
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1997: Lin S J; Kim D W; Ryugo Y; Wakagi T; Matsuzawa H
Increase of the protease activity of aqualysin I, a thermostable serine protease, by replacing Asn219 near the catalytic residue Ser222.
Bioscience, biotechnology, and biochemistry 1997;61(4):718-9.
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1997: Fujii T; Hata Y; Oozeki M; Moriyama H; Wakagi T; Tanaka N; Oshima T
The crystal structure of zinc-containing ferredoxin from the thermoacidophilic archaeon Sulfolobus sp. strain 7.
Biochemistry 1997;36(6):1505-13.
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