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Christopher Walsh

This is a preview profile on BiomedExperts - the first literature-based scientific social network. It brings the right researchers together and allows them to collaborate online. Collexis and Dell provide the BiomedExperts network of +1.5 Million pre-calculated profiles free of charge to researchers worldwide.

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Co-Publications
Name
29
Kelleher, Neil
20
Fischbach, Michael
18
Kahne, Daniel
13
Kohli, Rahul
12
Keating, Thomas
12
Losey, Heather
12
Gehring, AM
11
Garneau-Tsodikova, Sylvie
11
Kolter, Roberto
11
Oberthür, Markus
11
Vaillancourt, Frédéric
10
Yeh, Ellen
10
Yin, Jun
10
Khosla, Chaitan
10
Koglin, Alexander

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Publications

TOP 3
Liu Xinyu; Walsh Christopher T
Characterization of cyclo-acetoacetyl-L-tryptophan dimethylallyltransferase in cyclopiazonic acid biosynthesis: substrate promiscuity and site directed mutagenesis studies.
Hollenhorst Marie A; Clardy Jon; Walsh Christopher T
The ATP-dependent amide ligases DdaG and DdaF assemble the fumaramoyl-dipeptide scaffold of the dapdiamide antibiotics.
Frueh Dominique P; Leed Alison; Arthanari Haribabu; Koglin Alexander; Walsh Christopher T; Wagner Gerhard
Time-shared HSQC-NOESY for accurate distance constraints measured at high-field in (15)N-(13)C-ILV methyl labeled proteins.

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All Publications

2009: Liu Xinyu; Walsh Christopher T
Characterization of cyclo-acetoacetyl-L-tryptophan dimethylallyltransferase in cyclopiazonic acid biosynthesis: substrate promiscuity and site directed mutagenesis studies.
Biochemistry 2009;48(46):11032-44.
2009: Hollenhorst Marie A; Clardy Jon; Walsh Christopher T
The ATP-dependent amide ligases DdaG and DdaF assemble the fumaramoyl-dipeptide scaffold of the dapdiamide antibiotics.
Biochemistry 2009;48(43):10467-72.
2009: Frueh Dominique P; Leed Alison; Arthanari Haribabu; Koglin Alexander; Walsh Christopher T; Wagner Gerhard
Time-shared HSQC-NOESY for accurate distance constraints measured at high-field in (15)N-(13)C-ILV methyl labeled proteins.
Journal of biomolecular NMR 2009;45(3):311-8.
2009: Matthews Megan L; Neumann Christopher S; Miles Linde A; Grove Tyler L; Booker Squire J; Krebs Carsten; Walsh Christopher T; Bollinger J Martin
Substrate positioning controls the partition between halogenation and hydroxylation in the aliphatic halogenase, SyrB2.
Proceedings of the National Academy of Sciences of the United States of America 2009;106(42):17723-8.
2009: Balskus Emily P; Walsh Christopher T
An enzymatic cyclopentyl[b]indole formation involved in scytonemin biosynthesis.
Journal of the American Chemical Society 2009;131(41):14648-9.
2009: Walsh Christopher T; Wright Gerard D
Antimicrobials.
Current opinion in microbiology 2009;12(5):473-5.
2009: Frueh Dominique P; Arthanari Haribabu; Koglin Alexander; Walsh Christopher T; Wagner Gerhard
A double TROSY hNCAnH experiment for efficient assignment of large and challenging proteins.
Journal of the American Chemical Society 2009;131(36):12880-1.
2009: Liu Xinyu; Walsh Christopher T
Cyclopiazonic acid biosynthesis in Aspergillus sp.: characterization of a reductase-like R* domain in cyclopiazonate synthetase that forms and releases cyclo-acetoacetyl-L-tryptophan.
Biochemistry 2009;48(36):8746-57.
2009: Koglin Alexander; Walsh Christopher T
Structural insights into nonribosomal peptide enzymatic assembly lines.
Natural product reports 2009;26(8):987-1000.
2009: Wuest William M; Sattely Elizabeth S; Walsh Christopher T
Three siderophores from one bacterial enzymatic assembly line.
Journal of the American Chemical Society 2009;131(14):5056-7.
2009: Wong Cintyu; Fujimori Danica Galonic; Walsh Christopher T; Drennan Catherine L
Structural analysis of an open active site conformation of nonheme iron halogenase CytC3.
Journal of the American Chemical Society 2009;131(13):4872-9.
2009: Strieter Eric R; Koglin Alexander; Aron Zachary D; Walsh Christopher T
Cascade reactions during coronafacic acid biosynthesis: elongation, cyclization, and functionalization during Cfa7-catalyzed condensation.
Journal of the American Chemical Society 2009;131(6):2113-5.
2009: Walsh Christopher T; Fischbach Michael A
Repurposing libraries of eukaryotic protein kinase inhibitors for antibiotic discovery.
Proceedings of the National Academy of Sciences of the United States of America 2009;106(6):1689-90.
2009: Nolan Elizabeth M; Walsh Christopher T
How nature morphs peptide scaffolds into antibiotics.
Chembiochem : a European journal of chemical biology 2009;10(1):34-53.
2008: Gómez García Inmaculada; Freel Meyers Caren L; Walsh Christopher T; Lawson David M
Crystallization and preliminary X-ray analysis of the O-carbamoyltransferase NovN from the novobiocin-biosynthetic cluster of Streptomyces spheroides.
Acta crystallographica. Section F, Structural biology and crystallization communications 2008;64(Pt 11):1000-2.
2008: Neumann Christopher S; Walsh Christopher T
Biosynthesis of (-)-(1S,2R)-allocoronamic acyl thioester by an Fe(II)-dependent halogenase and a cyclopropane-forming flavoprotein.
Journal of the American Chemical Society 2008;130(43):14022-3.
2008: Heemstra John R; Walsh Christopher T
Tandem action of the O2- and FADH2-dependent halogenases KtzQ and KtzR produce 6,7-dichlorotryptophan for kutzneride assembly.
Journal of the American Chemical Society 2008;130(43):14024-5.
2008: Singh Gitanjali M; Fortin Pascal D; Koglin Alexander; Walsh Christopher T
beta-Hydroxylation of the aspartyl residue in the phytotoxin syringomycin E: characterization of two candidate hydroxylases AspH and SyrP in Pseudomonas syringae.
Biochemistry 2008;47(43):11310-20.
2008: Liu Xinyu; Fortin Pascal D; Walsh Christopher T
Andrimid producers encode an acetyl-CoA carboxyltransferase subunit resistant to the action of the antibiotic.
Proceedings of the National Academy of Sciences of the United States of America 2008;105(36):13321-6.
2008: Calderone Christopher T; Bumpus Stefanie B; Kelleher Neil L; Walsh Christopher T; Magarvey Nathan A
A ketoreductase domain in the PksJ protein of the bacillaene assembly line carries out both alpha- and beta-ketone reduction during chain growth.
Proceedings of the National Academy of Sciences of the United States of America 2008;105(35):12809-14.
2008: Sattely Elizabeth S; Walsh Christopher T
A latent oxazoline electrophile for N-O-C bond formation in pseudomonine biosynthesis.
Journal of the American Chemical Society 2008;130(37):12282-4.
2008: Bumpus Stefanie B; Magarvey Nathan A; Kelleher Neil L; Walsh Christopher T; Calderone Christopher T
Polyunsaturated fatty-acid-like trans-enoyl reductases utilized in polyketide biosynthesis.
Journal of the American Chemical Society 2008;130(35):11614-6.
2008: Nolan Elizabeth M; Walsh Christopher T
Investigations of the MceIJ-catalyzed posttranslational modification of the microcin E492 C-terminus: linkage of ribosomal and nonribosomal peptides to form "trojan horse" antibiotics.
Biochemistry 2008;47(35):9289-99.
2008: Magarvey Nathan A; Fortin Pascal D; Thomas Paul M; Kelleher Neil L; Walsh Christopher T
Gatekeeping versus promiscuity in the early stages of the andrimid biosynthetic assembly line.
ACS chemical biology 2008;3(9):542-54.
2008: Frueh Dominique P; Arthanari Haribabu; Koglin Alexander; Vosburg David A; Bennett Andrew E; Walsh Christopher T; Wagner Gerhard
Dynamic thiolation-thioesterase structure of a non-ribosomal peptide synthetase.
Nature 2008;454(7206):903-6.
2008: Sattely Elizabeth S; Fischbach Michael A; Walsh Christopher T
Total biosynthesis: in vitro reconstitution of polyketide and nonribosomal peptide pathways.
Natural product reports 2008;25(4):757-93.
2008: Zhou Zhe; Koglin Alexander; Wang Yu; McMahon Andrew P; Walsh Christopher T
An eight residue fragment of an acyl carrier protein suffices for post-translational introduction of fluorescent pantetheinyl arms in protein modification in vitro and in vivo.
Journal of the American Chemical Society 2008;130(30):9925-30.
2008: Walsh Christopher T; Nolan Elizabeth M
Morphing peptide backbones into heterocycles.
Proceedings of the National Academy of Sciences of the United States of America 2008;105(15):5655-6.
2008: Roush Rebecca F; Nolan Elizabeth M; Löhr Frank; Walsh Christopher T
Maturation of an Escherichia coli ribosomal peptide antibiotic by ATP-consuming N-P bond formation in microcin C7.
Journal of the American Chemical Society 2008;130(11):3603-9.
2008: Fischbach Michael A; Walsh Christopher T; Clardy Jon
The evolution of gene collectives: How natural selection drives chemical innovation.
Proceedings of the National Academy of Sciences of the United States of America 2008;105(12):4601-8.
2008: Ryan Katherine S; Balibar Carl J; Turo Kaitlyn E; Walsh Christopher T; Drennan Catherine L
The violacein biosynthetic enzyme VioE shares a fold with lipoprotein transporter proteins.
The Journal of biological chemistry 2008;283(10):6467-75.
2008: Neumann Christopher S; Fujimori Danica Galonic; Walsh Christopher T
Halogenation strategies in natural product biosynthesis.
Chemistry & biology 2008;15(2):99-109.
2008: Balibar Carl J; Walsh Christopher T
From thioesters to amides and back: condensation domain reversibility in the biosynthesis of vibriobactin.
Chembiochem : a European journal of chemical biology 2008;9(1):42-5.
2008: Walsh Christopher T; Fischbach Michael A
Inhibitors of sterol biosynthesis as Staphylococcus aureus antibiotics.
Angewandte Chemie (International ed. in English) 2008;47(31):5700-2.
2007: Read Jay A; Walsh Christopher T
The lyngbyatoxin biosynthetic assembly line: chain release by four-electron reduction of a dipeptidyl thioester to the corresponding alcohol.
Journal of the American Chemical Society 2007;129(51):15762-3.
2007: Nolan Elizabeth M; Fischbach Michael A; Koglin Alexander; Walsh Christopher T
Biosynthetic tailoring of microcin E492m: post-translational modification affords an antibacterial siderophore-peptide conjugate.
Journal of the American Chemical Society 2007;129(46):14336-47.
2007: Neidig Michael L; Brown Christina D; Light Kenneth M; Fujimori Danica Galonic; Nolan Elizabeth M; Price John C; Barr Eric W; Bollinger J Martin; Krebs Carsten; Walsh Christopher T; Solomon Edward I
CD and MCD of CytC3 and taurine dioxygenase: role of the facial triad in alpha-KG-dependent oxygenases.
Journal of the American Chemical Society 2007;129(46):14224-31.
2007: Fujimori Danica Galonic; Barr Eric W; Matthews Megan L; Koch Gretchen M; Yonce J Ryan; Walsh Christopher T; Bollinger J Martin; Krebs Carsten; Riggs-Gelasco Pamela J
Spectroscopic evidence for a high-spin Br-Fe(IV)-oxo intermediate in the alpha-ketoglutarate-dependent halogenase CytC3 from Streptomyces.
Journal of the American Chemical Society 2007;129(44):13408-9.
2007: Fujimori Danica Galonic; Hrvatin Sinisa; Neumann Christopher S; Strieker Matthias; Marahiel Mohamed A; Walsh Christopher T
Cloning and characterization of the biosynthetic gene cluster for kutznerides.
Proceedings of the National Academy of Sciences of the United States of America 2007;104(42):16498-503.
2007: Fujimori Danica Galonic; Walsh Christopher T
What's new in enzymatic halogenations.
Current opinion in chemical biology 2007;11(5):553-60.
2007: Ryan Katherine S; Howard-Jones Annaleise R; Hamill Michael J; Elliott Sean J; Walsh Christopher T; Drennan Catherine L
Crystallographic trapping in the rebeccamycin biosynthetic enzyme RebC.
Proceedings of the National Academy of Sciences of the United States of America 2007;104(39):15311-6.
2007: Howard-Jones Annaleise R; Walsh Christopher T
Nonenzymatic oxidative steps accompanying action of the cytochrome P450 enzymes StaP and RebP in the biosynthesis of staurosporine and rebeccamycin.
Journal of the American Chemical Society 2007;129(36):11016-7.
2007: Balibar Carl J; Howard-Jones Annaleise R; Walsh Christopher T
Terrequinone A biosynthesis through L-tryptophan oxidation, dimerization and bisprenylation.
Nature chemical biology 2007;3(9):584-92.
2007: Leimkuhler Catherine; Fridman Micha; Lupoli Tania; Walker Suzanne; Walsh Christopher T; Kahne Daniel
Characterization of rhodosaminyl transfer by the AknS/AknT glycosylation complex and its use in reconstituting the biosynthetic pathway of aclacinomycin A.
Journal of the American Chemical Society 2007;129(34):10546-50.
2007: Howard-Jones Annaleise R; Kruger Ryan G; Lu Wei; Tao Junhua; Leimkuhler Catherine; Kahne Daniel; Walsh Christopher T
Kinetic analysis of teicoplanin glycosyltransferases and acyltransferase reveal ordered tailoring of aglycone scaffold to reconstitute mature teicoplanin.
Journal of the American Chemical Society 2007;129(33):10082-3.
2007: Fortin Pascal D; Walsh Christopher T; Magarvey Nathan A
A transglutaminase homologue as a condensation catalyst in antibiotic assembly lines.
Nature 2007;448(7155):824-7.
2007: Calderone Christopher T; Iwig David F; Dorrestein Pieter C; Kelleher Neil L; Walsh Christopher T
Incorporation of nonmethyl branches by isoprenoid-like logic: multiple beta-alkylation events in the biosynthesis of myxovirescin A1.
Chemistry & biology 2007;14(7):835-46.
2007: Fischbach Michael A; Lai Jonathan R; Roche Eric D; Walsh Christopher T; Liu David R
Directed evolution can rapidly improve the activity of chimeric assembly-line enzymes.
Proceedings of the National Academy of Sciences of the United States of America 2007;104(29):11951-6.
2007: Zhou Zhe; Lai Jonathan R; Walsh Christopher T
Directed evolution of aryl carrier proteins in the enterobactin synthetase.
Proceedings of the National Academy of Sciences of the United States of America 2007;104(28):11621-6.
2007: Fridman Micha; Balibar Carl J; Lupoli Tania; Kahne Daniel; Walsh Christopher T; Garneau-Tsodikova Sylvie
Chemoenzymatic formation of novel aminocoumarin antibiotics by the enzymes CouN1 and CouN7.
Biochemistry 2007;46(28):8462-71.
2007: Krebs Carsten; Galonic Fujimori Danica; Walsh Christopher T; Bollinger J Martin
Non-heme Fe(IV)-oxo intermediates.
Accounts of chemical research 2007;40(7):484-92.
2007: Balibar Carl J; Garneau-Tsodikova Sylvie; Walsh Christopher T
Covalent CouN7 enzyme intermediate for acyl group shuttling in aminocoumarin biosynthesis.
Chemistry & biology 2007;14(6):679-90.
2007: Strieter Eric R; Vaillancourt Frédéric H; Walsh Christopher T
CmaE: a transferase shuttling aminoacyl groups between carrier protein domains in the coronamic acid biosynthetic pathway.
Biochemistry 2007;46(25):7549-57.
2007: Pacholec Michelle; Sello Jason K; Walsh Christopher T; Thomas Michael G
Formation of an aminoacyl-S-enzyme intermediate is a key step in the biosynthesis of chloramphenicol.
Organic & biomolecular chemistry 2007;5(11):1692-4.
2007: Walsh Christopher T
Revealing coupling patterns in isoprenoid alkylation biocatalysis.
ACS chemical biology 2007;2(5):296-8.
2007: Hansen Darren B; Bumpus Stefanie B; Aron Zachary D; Kelleher Neil L; Walsh Christopher T
The loading module of mycosubtilin: an adenylation domain with fatty acid selectivity.
Journal of the American Chemical Society 2007;129(20):6366-7.
2007: Zhou Zhe; Cironi Pablo; Lin Alison J; Xu Yangqing; Hrvatin Sinisa; Golan David E; Silver Pamela A; Walsh Christopher T; Yin Jun
Genetically encoded short peptide tags for orthogonal protein labeling by Sfp and AcpS phosphopantetheinyl transferases.
ACS chemical biology 2007;2(5):337-46.
2007: Aron Zachary D; Fortin Pascal D; Calderone Christopher T; Walsh Christopher T
FenF: servicing the Mycosubtilin synthetase assembly line in trans.
Chembiochem : a European journal of chemical biology 2007;8(6):613-6.
2007: Yin Jun; Straight Paul D; Hrvatin Sinisa; Dorrestein Pieter C; Bumpus Stefanie B; Jao Cindy; Kelleher Neil L; Kolter Roberto; Walsh Christopher T
Genome-wide high-throughput mining of natural-product biosynthetic gene clusters by phage display.
Chemistry & biology 2007;14(3):303-12.
2007: Taga Michiko E; Larsen Nicholas A; Howard-Jones Annaleise R; Walsh Christopher T; Walker Graham C
BluB cannibalizes flavin to form the lower ligand of vitamin B12.
Nature 2007;446(7134):449-53.
2007: Stevenson Clare E M; Freel Meyers Caren L; Walsh Christopher T; Lawson David M
Crystallization and preliminary X-ray analysis of the O-methyltransferase NovP from the novobiocin-biosynthetic cluster of Streptomyces spheroides.
Acta crystallographica. Section F, Structural biology and crystallization communications 2007;63(Pt 3):236-8.
2007: Yeh Ellen; Blasiak Leah C; Koglin Alexander; Drennan Catherine L; Walsh Christopher T
Chlorination by a long-lived intermediate in the mechanism of flavin-dependent halogenases.
Biochemistry 2007;46(5):1284-92.
2007: Galonic Danica P; Barr Eric W; Walsh Christopher T; Bollinger J Martin; Krebs Carsten
Two interconverting Fe(IV) intermediates in aliphatic chlorination by the halogenase CytC3.
Nature chemical biology 2007;3(2):113-6.
2007: Singh Gitanjali M; Vaillancourt Frédéric H; Yin Jun; Walsh Christopher T
Characterization of SyrC, an aminoacyltransferase shuttling threonyl and chlorothreonyl residues in the syringomycin biosynthetic assembly line.
Chemistry & biology 2007;14(1):31-40.
2007: Butcher Rebecca A; Schroeder Frank C; Fischbach Michael A; Straight Paul D; Kolter Roberto; Walsh Christopher T; Clardy Jon
The identification of bacillaene, the product of the PksX megacomplex in Bacillus subtilis.
Proceedings of the National Academy of Sciences of the United States of America 2007;104(5):1506-9.
2007: Kelly Wendy L; Boyne Michael T; Yeh Ellen; Vosburg David A; Galonic Danica P; Kelleher Neil L; Walsh Christopher T
Characterization of the aminocarboxycyclopropane-forming enzyme CmaC.
Biochemistry 2007;46(2):359-68.
2007: Straight Paul D; Fischbach Michael A; Walsh Christopher T; Rudner David Z; Kolter Roberto
A singular enzymatic megacomplex from Bacillus subtilis.
Proceedings of the National Academy of Sciences of the United States of America 2007;104(1):305-10.
2006: Balibar Carl J; Walsh Christopher T
In vitro biosynthesis of violacein from L-tryptophan by the enzymes VioA-E from Chromobacterium violaceum.
Biochemistry 2006;45(51):15444-57.
2006: Clardy Jon; Fischbach Michael A; Walsh Christopher T
New antibiotics from bacterial natural products.
Nature biotechnology 2006;24(12):1541-50.
2006: Balibar Carl J; Walsh Christopher T
GliP, a multimodular nonribosomal peptide synthetase in Aspergillus fumigatus, makes the diketopiperazine scaffold of gliotoxin.
Biochemistry 2006;45(50):15029-38.
2006: Lai Jonathan R; Koglin Alexander; Walsh Christopher T
Carrier protein structure and recognition in polyketide and nonribosomal peptide biosynthesis.
Biochemistry 2006;45(50):14869-79.
2006: Ueki Masashi; Galonic Danica P; Vaillancourt Frédéric H; Garneau-Tsodikova Sylvie; Yeh Ellen; Vosburg David A; Schroeder Frank C; Osada Hiroyuki; Walsh Christopher T
Enzymatic generation of the antimetabolite gamma,gamma-dichloroaminobutyrate by NRPS and mononuclear iron halogenase action in a streptomycete.
Chemistry & biology 2006;13(11):1183-91.
2006: Fischbach Michael A; Walsh Christopher T
Biochemistry. Directing biosynthesis.
Science (New York, N.Y.) 2006;314(5799):603-5.
2006: Fischbach Michael A; Lin Hening; Zhou Lu; Yu Yang; Abergel Rebecca J; Liu David R; Raymond Kenneth N; Wanner Barry L; Strong Roland K; Walsh Christopher T; Aderem Alan; Smith Kelly D
The pathogen-associated iroA gene cluster mediates bacterial evasion of lipocalin 2.
Proceedings of the National Academy of Sciences of the United States of America 2006;103(44):16502-7.
2006: Dorrestein Pieter C; Bumpus Stefanie B; Calderone Christopher T; Garneau-Tsodikova Sylvie; Aron Zachary D; Straight Paul D; Kolter Roberto; Walsh Christopher T; Kelleher Neil L
Facile detection of acyl and peptidyl intermediates on thiotemplate carrier domains via phosphopantetheinyl elimination reactions during tandem mass spectrometry.
Biochemistry 2006;45(42):12756-66.
2006: Garneau-Tsodikova Sylvie; Dorrestein Pieter C; Kelleher Neil L; Walsh Christopher T
Protein assembly line components in prodigiosin biosynthesis: characterization of PigA,G,H,I,J.
Journal of the American Chemical Society 2006;128(39):12600-1.
2006: Hicks Leslie M; Balibar Carl J; Walsh Christopher T; Kelleher Neil L; Hillson Nathan J
Probing intra- versus interchain kinetic preferences of L-Thr acylation on dimeric VibF with mass spectrometry.
Biophysical journal 2006;91(7):2609-19.
2006: Howard-Jones Annaleise R; Walsh Christopher T
Staurosporine and rebeccamycin aglycones are assembled by the oxidative action of StaP, StaC, and RebC on chromopyrrolic acid.
Journal of the American Chemical Society 2006;128(37):12289-98.
2006: Zhou Zhe; Lai Jonathan R; Walsh Christopher T
Interdomain communication between the thiolation and thioesterase domains of EntF explored by combinatorial mutagenesis and selection.
Chemistry & biology 2006;13(8):869-79.
2006: Lai Jonathan R; Fischbach Michael A; Liu David R; Walsh Christopher T
Localized protein interaction surfaces on the EntB carrier protein revealed by combinatorial mutagenesis and selection.
Journal of the American Chemical Society 2006;128(34):11002-3.
2006: Larsen N A; Lin H; Wei R; Fischbach M A; Walsh C T
Structural characterization of enterobactin hydrolase IroE.
Biochemistry 2006;45(34):10184-90.
2006: Magarvey Nathan A; Ehling-Schulz Monika; Walsh Christopher T
Characterization of the cereulide NRPS alpha-hydroxy acid specifying modules: activation of alpha-keto acids and chiral reduction on the assembly line.
Journal of the American Chemical Society 2006;128(33):10698-9.
2006: Fischbach Michael A; Walsh Christopher T
Assembly-line enzymology for polyketide and nonribosomal Peptide antibiotics: logic, machinery, and mechanisms.
Chemical reviews 2006;106(8):3468-96.
2006: Vaillancourt Frédéric H; Yeh Ellen; Vosburg David A; Garneau-Tsodikova Sylvie; Walsh Christopher T
Nature's inventory of halogenation catalysts: oxidative strategies predominate.
Chemical reviews 2006;106(8):3364-78.
2006: Walsh Christopher T; Garneau-Tsodikova Sylvie; Howard-Jones Annaleise R
Biological formation of pyrroles: nature's logic and enzymatic machinery.
Natural product reports 2006;23(4):517-31.
2006: Lin Hening; Fischbach Michael A; Gatto Gregory J; Liu David R; Walsh Christopher T
Bromoenterobactins as potent inhibitors of a pathogen-associated, siderophore-modifying C-glycosyltransferase.
Journal of the American Chemical Society 2006;128(29):9324-5.
2006: Garneau-Tsodikova Sylvie; Stapon Anthony; Kahne Daniel; Walsh Christopher T
Installation of the pyrrolyl-2-carboxyl pharmacophore by CouN1 and CouN7 in the late biosynthetic steps of the aminocoumarin antibiotics clorobiocin and coumermycin A1.
Biochemistry 2006;45(28):8568-78.
2006: Yeh Ellen; Cole Lindsay J; Barr Eric W; Bollinger J Martin; Ballou David P; Walsh Christopher T
Flavin redox chemistry precedes substrate chlorination during the reaction of the flavin-dependent halogenase RebH.
Biochemistry 2006;45(25):7904-12.
2006: Calderone Christopher T; Kowtoniuk Walter E; Kelleher Neil L; Walsh Christopher T; Dorrestein Pieter C
Convergence of isoprene and polyketide biosynthetic machinery: isoprenyl-S-carrier proteins in the pksX pathway of Bacillus subtilis.
Proceedings of the National Academy of Sciences of the United States of America 2006;103(24):8977-82.
2006: Frueh Dominique P; Sun Zhen-Yu J; Vosburg David A; Walsh Christopher T; Hoch Jeffrey C; Wagner Gerhard
Non-uniformly sampled double-TROSY hNcaNH experiments for NMR sequential assignments of large proteins.
Journal of the American Chemical Society 2006;128(17):5757-63.
2006: Vaillancourt Frédéric H; Vosburg David A; Walsh Christopher T
Dichlorination and bromination of a threonyl-S-carrier protein by the non-heme Fe(II) halogenase SyrB2.
Chembiochem : a European journal of chemical biology 2006;7(5):748-52.
2006: Lai Jonathan R; Fischbach Michael A; Liu David R; Walsh Christopher T
A protein interaction surface in nonribosomal peptide synthesis mapped by combinatorial mutagenesis and selection.
Proceedings of the National Academy of Sciences of the United States of America 2006;103(14):5314-9.
2006: Jakimowicz Piotr; Tello Mónica; Meyers Caren L Freel; Walsh Christopher T; Buttner Mark J; Field Robert A; Lawson David M
The 1.6-A resolution crystal structure of NovW: a 4-keto-6-deoxy sugar epimerase from the novobiocin biosynthetic gene cluster of Streptomyces spheroides.
Proteins 2006;63(1):261-5.
2006: Galonic Danica P; Vaillancourt Frédéric H; Walsh Christopher T
Halogenation of unactivated carbon centers in natural product biosynthesis: trichlorination of leucine during barbamide biosynthesis.
Journal of the American Chemical Society 2006;128(12):3900-1.
2006: Blasiak Leah C; Vaillancourt Frédéric H; Walsh Christopher T; Drennan Catherine L
Crystal structure of the non-haem iron halogenase SyrB2 in syringomycin biosynthesis.
Nature 2006;440(7082):368-71.
2006: Gatto Gregory J; Boyne Michael T; Kelleher Neil L; Walsh Christopher T
Biosynthesis of pipecolic acid by RapL, a lysine cyclodeaminase encoded in the rapamycin gene cluster.
Journal of the American Chemical Society 2006;128(11):3838-47.
2006: Tello Mónica; Jakimowicz Piotr; Errey James C; Freel Meyers Caren L; Walsh Christopher T; Buttner Mark J; Lawson David M; Field Robert A
Characterisation of Streptomyces spheroides NovW and revision of its functional assignment to a dTDP-6-deoxy-D-xylo-4-hexulose 3-epimerase.
Chemical communications (Cambridge, England) 2006;(10):1079-81.
2006: Fischbach Michael A; Lin Hening; Liu David R; Walsh Christopher T
How pathogenic bacteria evade mammalian sabotage in the battle for iron.
Nature chemical biology 2006;2(3):132-8.
2006: Dorrestein Pieter C; Blackhall Jonathan; Straight Paul D; Fischbach Michael A; Garneau-Tsodikova Sylvie; Edwards Daniel J; McLaughlin Shaun; Lin Myat; Gerwick William H; Kolter Roberto; Walsh Christopher T; Kelleher Neil L
Activity screening of carrier domains within nonribosomal peptide synthetases using complex substrate mixtures and large molecule mass spectrometry.
Biochemistry 2006;45(6):1537-46.
2006: Frueh Dominique P; Vosburg David A; Walsh Christopher T; Wagner Gerhard
Determination of all nOes in 1H-13C-Me-ILV-U-2H-15N proteins with two time-shared experiments.
Journal of biomolecular NMR 2006;34(1):31-40.
2006: Yin Jun; Lin Alison J; Golan David E; Walsh Christopher T
Site-specific protein labeling by Sfp phosphopantetheinyl transferase.
Nature protocols 2006;1(1):280-5.
2005: Howard-Jones Annaleise R; Walsh Christopher T
Enzymatic generation of the chromopyrrolic acid scaffold of rebeccamycin by the tandem action of RebO and RebD.
Biochemistry 2005;44(48):15652-63.
2005: Walsh Christopher T; Garneau-Tsodikova Sylvie; Gatto Gregory J
Protein posttranslational modifications: the chemistry of proteome diversifications.
Angewandte Chemie (International ed. in English) 2005;44(45):7342-72.
2005: Balibar Carl J; Vaillancourt Frédéric H; Walsh Christopher T
Generation of D amino acid residues in assembly of arthrofactin by dual condensation/epimerization domains.
Chemistry & biology 2005;12(11):1189-200.
2005: Pacholec Michelle; Tao Junhua; Walsh Christopher T
CouO and NovO: C-methyltransferases for tailoring the aminocoumarin scaffold in coumermycin and novobiocin antibiotic biosynthesis.
Biochemistry 2005;44(45):14969-76.
2005: Aron Zachary D; Dorrestein Pieter C; Blackhall Jonathan R; Kelleher Neil L; Walsh Christopher T
Characterization of a new tailoring domain in polyketide biogenesis: the amine transferase domain of MycA in the mycosubtilin gene cluster.
Journal of the American Chemical Society 2005;127(43):14986-7.
2005: McLoughlin Shaun M; Mazur Matthew T; Miller Leah M; Yin Jun; Liu Fei; Walsh Christopher T; Kelleher Neil L
Chemoenzymatic approaches for streamlined detection of active site modifications on thiotemplate assembly lines using mass spectrometry.
Biochemistry 2005;44(43):14159-69.
2005: Yin Jun; Straight Paul D; McLoughlin Shaun M; Zhou Zhe; Lin Alison J; Golan David E; Kelleher Neil L; Kolter Roberto; Walsh Christopher T
Genetically encoded short peptide tag for versatile protein labeling by Sfp phosphopantetheinyl transferase.
Proceedings of the National Academy of Sciences of the United States of America 2005;102(44):15815-20.
2005: Yuan Yanqiu; Chung Hak Suk; Leimkuhler Catherine; Walsh Christopher T; Kahne Daniel; Walker Suzanne
In vitro reconstitution of EryCIII activity for the preparation of unnatural macrolides.
Journal of the American Chemical Society 2005;127(41):14128-9.
2005: Kelly Wendy L; Hillson Nathan J; Walsh Christopher T
Excision of the epothilone synthetase B cyclization domain and demonstration of in trans condensation/cyclodehydration activity.
Biochemistry 2005;44(40):13385-93.
2005: Yin Jun; Lin Alison J; Buckett Peter D; Wessling-Resnick Marianne; Golan David E; Walsh Christopher T
Single-cell FRET imaging of transferrin receptor trafficking dynamics by Sfp-catalyzed, site-specific protein labeling.
Chemistry & biology 2005;12(9):999-1006.
2005: Pacholec M; Hillson N J; Walsh C T
NovJ/NovK catalyze benzylic oxidation of a beta-hydroxyl tyrosyl-S-pantetheinyl enzyme during aminocoumarin ring formation in novobiocin biosynthesis.
Biochemistry 2005;44(38):12819-26.
2005: Dorrestein Pieter C; Yeh Ellen; Garneau-Tsodikova Sylvie; Kelleher Neil L; Walsh Christopher T
Dichlorination of a pyrrolyl-S-carrier protein by FADH2-dependent halogenase PltA during pyoluteorin biosynthesis.
Proceedings of the National Academy of Sciences of the United States of America 2005;102(39):13843-8.
2005: Walsh Christopher T
Natural insights for chemical biologists.
Nature chemical biology 2005;1(3):122-4.
2005: Vaillancourt Frédéric H; Yeh Ellen; Vosburg David A; O'Connor Sarah E; Walsh Christopher T
Cryptic chlorination by a non-haem iron enzyme during cyclopropyl amino acid biosynthesis.
Nature 2005;436(7054):1191-4.
2005: Lin Hening; Fischbach Michael A; Liu David R; Walsh Christopher T
In vitro characterization of salmochelin and enterobactin trilactone hydrolases IroD, IroE, and Fes.
Journal of the American Chemical Society 2005;127(31):11075-84.
2005: Oberthür Markus; Leimkuhler Catherine; Kruger Ryan G; Lu Wei; Walsh Christopher T; Kahne Daniel
A systematic investigation of the synthetic utility of glycopeptide glycosyltransferases.
Journal of the American Chemical Society 2005;127(30):10747-52.
2005: Vaillancourt Frédéric H; Yin Jun; Walsh Christopher T
SyrB2 in syringomycin E biosynthesis is a nonheme FeII alpha-ketoglutarate- and O2-dependent halogenase.
Proceedings of the National Academy of Sciences of the United States of America 2005;102(29):10111-6.
2005: Lu Wei; Leimkuhler Catherine; Gatto Gregory J; Kruger Ryan G; Oberthür Markus; Kahne Daniel; Walsh Christopher T
AknT is an activating protein for the glycosyltransferase AknS in L-aminodeoxysugar transfer to the aglycone of aclacinomycin A.
Chemistry & biology 2005;12(5):527-34.
2005: Gatto Gregory J; McLoughlin Shaun M; Kelleher Neil L; Walsh Christopher T
Elucidating the substrate specificity and condensation domain activity of FkbP, the FK520 pipecolate-incorporating enzyme.
Biochemistry 2005;44(16):5993-6002.
2005: Pacholec Michelle; Freel Meyers Caren L; Oberthür Markus; Kahne Daniel; Walsh Christopher T
Characterization of the aminocoumarin ligase SimL from the simocyclinone pathway and tandem incubation with NovM,P,N from the novobiocin pathway.
Biochemistry 2005;44(12):4949-56.
2005: Yeh Ellen; Garneau Sylvie; Walsh Christopher T
Robust in vitro activity of RebF and RebH, a two-component reductase/halogenase, generating 7-chlorotryptophan during rebeccamycin biosynthesis.
Proceedings of the National Academy of Sciences of the United States of America 2005;102(11):3960-5.
2005: Garneau Sylvie; Dorrestein Pieter C; Kelleher Neil L; Walsh Christopher T
Characterization of the formation of the pyrrole moiety during clorobiocin and coumermycin A1 biosynthesis.
Biochemistry 2005;44(8):2770-80.
2005: Kruger Ryan G; Lu Wei; Oberthür Markus; Tao Junhua; Kahne Daniel; Walsh Christopher T
Tailoring of glycopeptide scaffolds by the acyltransferases from the teicoplanin and A-40,926 biosynthetic operons.
Chemistry & biology 2005;12(1):131-40.
2005: Fischbach Michael A; Lin Hening; Liu David R; Walsh Christopher T
In vitro characterization of IroB, a pathogen-associated C-glycosyltransferase.
Proceedings of the National Academy of Sciences of the United States of America 2005;102(3):571-6.
2005: Vosburg D A; Walsh C T
Natural product biosynthetic assembly lines: prospects and challenges for reprogramming.
Ernst Schering Research Foundation workshop 2005;(51):261-84.
2004: Lin Hening; Thayer Desiree A; Wong Chi-Huey; Walsh Christopher T
Macrolactamization of glycosylated peptide thioesters by the thioesterase domain of tyrocidine synthetase.
Chemistry & biology 2004;11(12):1635-42.
2004: Schneider Tanya L; Walsh Christopher T
Portability of oxidase domains in nonribosomal peptide synthetase modules.
Biochemistry 2004;43(50):15946-55.
2004: Yeh Ellen; Lin Hening; Clugston Susan L; Kohli Rahul M; Walsh Christopher T
Enhanced macrocyclizing activity of the thioesterase from tyrocidine synthetase in presence of nonionic detergent.
Chemistry & biology 2004;11(11):1573-82.
2004: Liu Fei; Garneau Sylvie; Walsh Christopher T
Hybrid nonribosomal peptide-polyketide interfaces in epothilone biosynthesis: minimal requirements at N and C termini of EpoB for elongation.
Chemistry & biology 2004;11(11):1533-42.
2004: Freel Meyers Caren L; Oberthür Markus; Heide Lutz; Kahne Daniel; Walsh Christopher T
Assembly of dimeric variants of coumermycins by tandem action of the four biosynthetic enzymes CouL, CouM, CouP, and NovN.
Biochemistry 2004;43(47):15022-36.
2004: Lin Hening; Walsh Christopher T
A chemoenzymatic approach to glycopeptide antibiotics.
Journal of the American Chemical Society 2004;126(43):13998-4003.
2004: Yin Jun; Liu Fei; Schinke Martina; Daly Christian; Walsh Christopher T
Phagemid encoded small molecules for high throughput screening of chemical libraries.
Journal of the American Chemical Society 2004;126(42):13570-1.
2004: Reimmann Cornelia; Patel Hiten M; Walsh Christopher T; Haas Dieter
PchC thioesterase optimizes nonribosomal biosynthesis of the peptide siderophore pyochelin in Pseudomonas aeruginosa.
Journal of bacteriology 2004;186(19):6367-73.
2004: Tseng Claire C; Vaillancourt Frédéric H; Bruner Steven D; Walsh Christopher T
DpgC is a metal- and cofactor-free 3,5-dihydroxyphenylacetyl-CoA 1,2-dioxygenase in the vancomycin biosynthetic pathway.
Chemistry & biology 2004;11(9):1195-203.
2004: Yeh Ellen; Kohli Rahul M; Bruner Steven D; Walsh Christopher T
Type II thioesterase restores activity of a NRPS module stalled with an aminoacyl-S-enzyme that cannot be elongated.
Chembiochem : a European journal of chemical biology 2004;5(9):1290-3.
2004: Hillson Nathan J; Balibar Carl J; Walsh Christopher T
Catalytically inactive condensation domain C1 is responsible for the dimerization of the VibF subunit of vibriobactin synthetase.
Biochemistry 2004;43(35):11344-51.
2004: Lee Ho Young; Chung Hak Suk; Hang Chao; Khosla Chaitan; Walsh Christopher T; Kahne Daniel; Walker Suzanne
Reconstitution and characterization of a new desosaminyl transferase, EryCIII, from the erythromycin biosynthetic pathway.
Journal of the American Chemical Society 2004;126(32):9924-5.
2004: Pi Na; Meyers Caren L Freel; Pacholec Michelle; Walsh Christopher T; Leary Julie A
Mass spectrometric characterization of a three-enzyme tandem reaction for assembly and modification of the novobiocin skeleton.
Proceedings of the National Academy of Sciences of the United States of America 2004;101(27):10036-41.
2004: Merkel Alexandra B; Major Louise L; Errey James C; Burkart Michael D; Field Robert A; Walsh Christopher T; Naismith James H
The position of a key tyrosine in dTDP-4-Keto-6-deoxy-D-glucose-5-epimerase (EvaD) alters the substrate profile for this RmlC-like enzyme.
The Journal of biological chemistry 2004;279(31):32684-91.
2004: Yin Jun; Liu Fei; Li Xiaohua; Walsh Christopher T
Labeling proteins with small molecules by site-specific posttranslational modification.
Journal of the American Chemical Society 2004;126(25):7754-5.
2004: Mulichak Anne M; Lu Wei; Losey Heather C; Walsh Christopher T; Garavito R Michael
Crystal structure of vancosaminyltransferase GtfD from the vancomycin biosynthetic pathway: interactions with acceptor and nucleotide ligands.
Biochemistry 2004;43(18):5170-80.
2004: Lu Wei; Leimkuhler Catherine; Oberthür Markus; Kahne Daniel; Walsh Christopher T
AknK is an L-2-deoxyfucosyltransferase in the biosynthesis of the anthracycline aclacinomycin A.
Biochemistry 2004;43(15):4548-58.
2004: Hicks Leslie M; O'Connor Sarah E; Mazur Matthew T; Walsh Christopher T; Kelleher Neil L
Mass spectrometric interrogation of thioester-bound intermediates in the initial stages of epothilone biosynthesis.
Chemistry & biology 2004;11(3):327-35.
2004: Lu Wei; Oberthür Markus; Leimkuhler Catherine; Tao Junhua; Kahne Daniel; Walsh Christopher T
Characterization of a regiospecific epivancosaminyl transferase GtfA and enzymatic reconstitution of the antibiotic chloroeremomycin.
Proceedings of the National Academy of Sciences of the United States of America 2004;101(13):4390-5.
2004: Walsh Christopher T
Polyketide and nonribosomal peptide antibiotics: modularity and versatility.
Science (New York, N.Y.) 2004;303(5665):1805-10.
2004: Tseng Claire C; McLoughlin Shaun M; Kelleher Neil L; Walsh Christopher T
Role of the active site cysteine of DpgA, a bacterial type III polyketide synthase.
Biochemistry 2004;43(4):970-80.
2004: Freel Meyers Caren L; Oberthür Markus; Xu Hui; Heide Lutz; Kahne Daniel; Walsh Christopher T
Characterization of NovP and NovN: completion of novobiocin biosynthesis by sequential tailoring of the noviosyl ring.
Angewandte Chemie (International ed. in English) 2004;43(1):67-70.
2003: Mazur Matthew T; Walsh Christopher T; Kelleher Neil L
Site-specific observation of acyl intermediate processing in thiotemplate biosynthesis by fourier transform mass spectrometry: the polyketide module of yersiniabactin synthetase.
Biochemistry 2003;42(46):13393-400.
2003: Pfeifer Blaine A; Wang Clay C C; Walsh Christopher T; Khosla Chaitan
Biosynthesis of Yersiniabactin, a complex polyketide-nonribosomal peptide, using Escherichia coli as a heterologous host.
Applied and environmental microbiology 2003;69(11):6698-702.
2003: Admiraal Suzanne J; Khosla Chaitan; Walsh Christopher T
A Switch for the transfer of substrate between nonribosomal peptide and polyketide modules of the rifamycin synthetase assembly line.
Journal of the American Chemical Society 2003;125(45):13664-5.
2003: Clugston Susan L; Sieber Stephan A; Marahiel Mohamed A; Walsh Christopher T
Chirality of peptide bond-forming condensation domains in nonribosomal peptide synthetases: the C5 domain of tyrocidine synthetase is a (D)C(L) catalyst.
Biochemistry 2003;42(41):12095-104.
2003: Patel Hiten M; Tao Junhua; Walsh Christopher T
Epimerization of an L-cysteinyl to a D-cysteinyl residue during thiazoline ring formation in siderophore chain elongation by pyochelin synthetase from Pseudomonas aeruginosa.
Biochemistry 2003;42(35):10514-27.
2003: Tao Junhua; Hu Shanghui; Pacholec Michelle; Walsh Christopher T
Synthesis of proposed oxidation-cyclization-methylation intermediates of the coumarin antibiotic biosynthetic pathway.
Organic letters 2003;5(18):3233-6.
2003: O'Connor Sarah E; Walsh Christopher T; Liu Fei
Biosynthesis of epothilone intermediates with alternate starter units: engineering polyketide-nonribosomal interfaces.
Angewandte Chemie (International ed. in English) 2003;42(33):3917-21.
2003: Schneider Tanya L; Shen Ben; Walsh Christopher T
Oxidase domains in epothilone and bleomycin biosynthesis: thiazoline to thiazole oxidation during chain elongation.
Biochemistry 2003;42(32):9722-30.
2003: Watanabe Kenji; Rude Mathew A; Walsh Christopher T; Khosla Chaitan
Engineered biosynthesis of an ansamycin polyketide precursor in Escherichia coli.
Proceedings of the National Academy of Sciences of the United States of America 2003;100(17):9774-8.
2003: Jakimowicz Piotr; Freel Meyers Caren L; Walsh Christopher T; Buttner Mark J; Lawson David M
Crystallization and preliminary X-ray studies on the putative dTDP sugar epimerase NovW from the novobiocin biosynthetic cluster of Streptomyces spheroides.
Acta crystallographica. Section D, Biological crystallography 2003;59(Pt 8):1507-9.
2003: Mulichak Anne M; Losey Heather C; Lu Wei; Wawrzak Zdzislaw; Walsh Christopher T; Garavito R Michael
Structure of the TDP-epi-vancosaminyltransferase GtfA from the chloroeremomycin biosynthetic pathway.
Proceedings of the National Academy of Sciences of the United States of America 2003;100(16):9238-43.
2003: Walsh Christopher T; O'Connor Sarah E; Schneider Tanya L
Polyketide-nonribosomal peptide epothilone antitumor agents: the EpoA, B, C subunits.
Journal of industrial microbiology & biotechnology 2003;30(8):448-55.
2003: Kohli Rahul M; Burke Martin D; Tao Junhua; Walsh Christopher T
Chemoenzymatic route to macrocyclic hybrid peptide/polyketide-like molecules.
Journal of the American Chemical Society 2003;125(24):7160-1.
2003: Walsh C T; Losey H C; Freel Meyers C L
Antibiotic glycosyltransferases.
Biochemical Society transactions 2003;31(Pt 3):487-92.
2003: Freel Meyers Caren L; Oberthür Markus; Anderson John W; Kahne Daniel; Walsh Christopher T
Initial characterization of novobiocic acid noviosyl transferase activity of NovM in biosynthesis of the antibiotic novobiocin.
Biochemistry 2003;42(14):4179-89.
2003: Boddy Christopher N; Schneider Tanya L; Hotta Kinya; Walsh Christopher T; Khosla Chaitan
Epothilone C macrolactonization and hydrolysis are catalyzed by the isolated thioesterase domain of epothilone polyketide synthase.
Journal of the American Chemical Society 2003;125(12):3428-9.
2003: Kohli Rahul M; Walsh Christopher T
Enzymology of acyl chain macrocyclization in natural product biosynthesis.
Chemical communications (Cambridge, England) 2003;(3):297-307.
2003: Hubbard Brian K; Walsh Christopher T
Vancomycin assembly: nature's way.
Angewandte Chemie (International ed. in English) 2003;42(7):730-65.
2003: Roche Eric D; Walsh Christopher T
Dissection of the EntF condensation domain boundary and active site residues in nonribosomal peptide synthesis.
Biochemistry 2003;42(5):1334-44.
2003: Hillson Nathan J; Walsh Christopher T
Dimeric structure of the six-domain VibF subunit of vibriobactin synthetase: mutant domain activity regain and ultracentrifugation studies.
Biochemistry 2003;42(3):766-75.
2002: Losey Heather C; Jiang Jiqing; Biggins John B; Oberthür Markus; Ye Xiang Yang; Dong Steven D; Kahne Daniel; Thorson Jon S; Walsh Christopher T
Incorporation of glucose analogs by GtfE and GtfD from the vancomycin biosynthetic pathway to generate variant glycopeptides.
Chemistry & biology 2002;9(12):1305-14.
2002: Tseng Claire C; Bruner Steven D; Kohli Rahul M; Marahiel Mohamed A; Walsh Christopher T; Sieber Stephan A
Characterization of the surfactin synthetase C-terminal thioesterase domain as a cyclic depsipeptide synthase.
Biochemistry 2002;41(45):13350-9.
2002: Schneider Tanya L; Walsh Christopher T; O'Connor Sarah E
Utilization of alternate substrates by the first three modules of the epothilone synthetase assembly line.
Journal of the American Chemical Society 2002;124(38):11272-3.
2002: Kohli Rahul M; Walsh Christopher T; Burkart Michael D
Biomimetic synthesis and optimization of cyclic peptide antibiotics.
Nature 2002;418(6898):658-61.
2002: Dong Steven D; Oberthür Markus; Losey Heather C; Anderson John W; Eggert Ulrike S; Peczuh Mark W; Walsh Christopher T; Kahne Daniel
The structural basis for induction of VanB resistance.
Journal of the American Chemical Society 2002;124(31):9064-5.
2002: Luo Lusong; Kohli Rahul M; Onishi Megumi; Linne Uwe; Marahiel Mohamed A; Walsh Christopher T
Timing of epimerization and condensation reactions in nonribosomal peptide assembly lines: kinetic analysis of phenylalanine activating elongation modules of tyrocidine synthetase B.
Biochemistry 2002;41(29):9184-96.
2002: Marshall C Gary; Burkart Michael D; Meray Robin K; Walsh Christopher T
Carrier protein recognition in siderophore-producing nonribosomal peptide synthetases.
Biochemistry 2002;41(26):8429-37.
2002: Merkel Alexandra B; Temple Genevieve K; Burkart Michael D; Losey Heather C; Beis Kostantinos; Walsh Christopher T; Naismith James H
Purification, crystallization and preliminary structural studies of dTDP-4-keto-6-deoxy-glucose-5-epimerase (EvaD) from Amycolatopsis orientalis, the fourth enzyme in the dTDP-L-epivancosamine biosynthetic pathway.
Acta crystallographica. Section D, Biological crystallography 2002;58(Pt 7):1226-8.
2002: Keating Thomas A; Marshall C Gary; Walsh Christopher T; Keating Amy E
The structure of VibH represents nonribosomal peptide synthetase condensation, cyclization and epimerization domains.
Nature structural biology 2002;9(7):522-6.
2002: Pootoolal Jeff; Thomas Michael G; Marshall C Gary; Neu John M; Hubbard Brian K; Walsh Christopher T; Wright Gerard D
Assembling the glycopeptide antibiotic scaffold: The biosynthesis of A47934 from Streptomyces toyocaensis NRRL15009.
Proceedings of the National Academy of Sciences of the United States of America 2002;99(13):8962-7.
2002: O'Connor Sarah E; Chen Huawei; Walsh Christopher T
Enzymatic assembly of epothilones: the EpoC subunit and reconstitution of the EpoA-ACP/B/C polyketide and nonribosomal peptide interfaces.
Biochemistry 2002;41(17):5685-94.
2002: Admiraal Suzanne J; Khosla Chaitan; Walsh Christopher T
The loading and initial elongation modules of rifamycin synthetase collaborate to produce mixed aryl ketide products.
Biochemistry 2002;41(16):5313-24.
2002: Bruner Steven D; Weber Thomas; Kohli Rahul M; Schwarzer Dirk; Marahiel Mohamed A; Walsh Christopher T; Stubbs Milton T
Structural basis for the cyclization of the lipopeptide antibiotic surfactin by the thioesterase domain SrfTE.
Structure (London, England : 1993) 2002;10(3):301-10.
2002: Miller Deborah Ann; Luo Lusong; Hillson Nathan; Keating Thomas A; Walsh Christopher T
Yersiniabactin synthetase: a four-protein assembly line producing the nonribosomal peptide/polyketide hybrid siderophore of Yersinia pestis.
Chemistry & biology 2002;9(3):333-44.
2002: Walsh Christopher T
Combinatorial biosynthesis of antibiotics: challenges and opportunities.
Chembiochem : a European journal of chemical biology 2002;3(2-3):125-34.
2002: Thomas Michael G; Burkart Michael D; Walsh Christopher T
Conversion of L-proline to pyrrolyl-2-carboxyl-S-PCP during undecylprodigiosin and pyoluteorin biosynthesis.
Chemistry & biology 2002;9(2):171-84.
2002: Kohli Rahul M; Takagi Junichi; Walsh Christopher T
The thioesterase domain from a nonribosomal peptide synthetase as a cyclization catalyst for integrin binding peptides.
Proceedings of the National Academy of Sciences of the United States of America 2002;99(3):1247-52.
2002: Chen Huawei; Hubbard Brian K; O'Connor Sarah E; Walsh Christopher T
Formation of beta-hydroxy histidine in the biosynthesis of nikkomycin antibiotics.
Chemistry & biology 2002;9(1):103-12.
2002: Marshall C Gary; Hillson Nathan J; Walsh Christopher T
Catalytic mapping of the vibriobactin biosynthetic enzyme VibF.
Biochemistry 2002;41(1):244-50.
2001: Chen H; Tseng C C; Hubbard B K; Walsh C T
Glycopeptide antibiotic biosynthesis: enzymatic assembly of the dedicated amino acid monomer (S)-3,5-dihydroxyphenylglycine.
Proceedings of the National Academy of Sciences of the United States of America 2001;98(26):14901-6.
2001: Luo L; Burkart M D; Stachelhaus T; Walsh C T
Substrate recognition and selection by the initiation module PheATE of gramicidin S synthetase.
Journal of the American Chemical Society 2001;123(45):11208-18.
2001: Walsh C T; Chen H; Keating T A; Hubbard B K; Losey H C; Luo L; Marshall C G; Miller D A; Patel H M
Tailoring enzymes that modify nonribosomal peptides during and after chain elongation on NRPS assembly lines.
Current opinion in chemical biology 2001;5(5):525-34.
2001: Chen H; Thomas M G; O'Connor S E; Hubbard B K; Burkart M D; Walsh C T
Aminoacyl-S-enzyme intermediates in beta-hydroxylations and alpha,beta-desaturations of amino acids in peptide antibiotics.
Biochemistry 2001;40(39):11651-9.
2001: Yuan W; Jia Y; Tian J; Snell K D; Müh U; Sinskey A J; Lambalot R H; Walsh C T; Stubbe J
Class I and III polyhydroxyalkanoate synthases from Ralstonia eutropha and Allochromatium vinosum: characterization and substrate specificity studies.
Archives of biochemistry and biophysics 2001;394(1):87-98.
2001: Chen H; O'Connor S; Cane D E; Walsh C T
Epothilone biosynthesis: assembly of the methylthiazolylcarboxy starter unit on the EpoB subunit.
Chemistry & biology 2001;8(9):899-912.
2001: Marshall C G; Burkart M D; Keating T A; Walsh C T
Heterocycle formation in vibriobactin biosynthesis: alternative substrate utilization and identification of a condensed intermediate.
Biochemistry 2001;40(35):10655-63.
2001: Forbes A J; Mazur M T; Patel H M; Walsh C T; Kelleher N L
Toward efficient analysis of >70 kDa proteins with 100% sequence coverage.
Proteomics 2001;1(8):927-33.
2001: Miller D A; Walsh C T; Luo L
C-methyltransferase and cyclization domain activity at the intraprotein PK/NRP switch point of yersiniabactin synthetase.
Journal of the American Chemical Society 2001;123(34):8434-5.
2001: Mulichak A M; Losey H C; Walsh C T; Garavito R M
Structure of the UDP-glucosyltransferase GtfB that modifies the heptapeptide aglycone in the biosynthesis of vancomycin group antibiotics.
Structure (London, England : 1993) 2001;9(7):547-57.
2001: Patel H M; Walsh C T
In vitro reconstitution of the Pseudomonas aeruginosa nonribosomal peptide synthesis of pyochelin: characterization of backbone tailoring thiazoline reductase and N-methyltransferase activities.
Biochemistry 2001;40(30):9023-31.
2001: Chiu H T; Hubbard B K; Shah A N; Eide J; Fredenburg R A; Walsh C T; Khosla C
Molecular cloning and sequence analysis of the complestatin biosynthetic gene cluster.
Proceedings of the National Academy of Sciences of the United States of America 2001;98(15):8548-53.
2001: Zamble D B; Miller D A; Heddle J G; Maxwell A; Walsh C T; Hollfelder F
In vitro characterization of DNA gyrase inhibition by microcin B17 analogs with altered bisheterocyclic sites.
Proceedings of the National Academy of Sciences of the United States of America 2001;98(14):7712-7.
2001: Kohli R M; Trauger J W; Schwarzer D; Marahiel M A; Walsh C T
Generality of peptide cyclization catalyzed by isolated thioesterase domains of nonribosomal peptide synthetases.
Biochemistry 2001;40(24):7099-108.
2001: Trauger J W; Kohli R M; Walsh C T
Cyclization of backbone-substituted peptides catalyzed by the thioesterase domain from the tyrocidine nonribosomal peptide synthetase.
Biochemistry 2001;40(24):7092-8.
2001: Admiraal S J; Walsh C T; Khosla C
The loading module of rifamycin synthetase is an adenylation-thiolation didomain with substrate tolerance for substituted benzoates.
Biochemistry 2001;40(20):6116-23.
2001: Luo L; Walsh C T
Kinetic analysis of three activated phenylalanyl intermediates generated by the initiation module PheATE of gramicidin S synthetase.
Biochemistry 2001;40(18):5329-37.
2001: Miller D A; Walsh C T
Yersiniabactin synthetase: probing the recognition of carrier protein domains by the catalytic heterocyclization domains, Cy1 and Cy2, in the chain-initiating HWMP2 subunit.
Biochemistry 2001;40(17):5313-21.
2001: Chen H; Walsh C T
Coumarin formation in novobiocin biosynthesis: beta-hydroxylation of the aminoacyl enzyme tyrosyl-S-NovH by a cytochrome P450 NovI.
Chemistry & biology 2001;8(4):301-12.
2001: Losey H C; Peczuh M W; Chen Z; Eggert U S; Dong S D; Pelczer I; Kahne D; Walsh C T
Tandem action of glycosyltransferases in the maturation of vancomycin and teicoplanin aglycones: novel glycopeptides.
Biochemistry 2001;40(15):4745-55.
2001: Heddle J G; Blance S J; Zamble D B; Hollfelder F; Miller D A; Wentzell L M; Walsh C T; Maxwell A
The antibiotic microcin B17 is a DNA gyrase poison: characterisation of the mode of inhibition.
Journal of molecular biology 2001;307(5):1223-34.
2001: Keating T A; Ehmann D E; Kohli R M; Marshall C G; Trauger J W; Walsh C T
Chain termination steps in nonribosomal peptide synthetase assembly lines: directed acyl-S-enzyme breakdown in antibiotic and siderophore biosynthesis.
Chembiochem : a European journal of chemical biology 2001;2(2):99-107.
2001: Reimmann C; Patel H M; Serino L; Barone M; Walsh C T; Haas D
Essential PchG-dependent reduction in pyochelin biosynthesis of Pseudomonas aeruginosa.
Journal of bacteriology 2001;183(3):813-20.
2001: Suo Z; Tseng C C; Walsh C T
Purification, priming, and catalytic acylation of carrier protein domains in the polyketide synthase and nonribosomal peptidyl synthetase modules of the HMWP1 subunit of yersiniabactin synthetase.
Proceedings of the National Academy of Sciences of the United States of America 2001;98(1):99-104.
2000: Hubbard B K; Thomas M G; Walsh C T
Biosynthesis of L-p-hydroxyphenylglycine, a non-proteinogenic amino acid constituent of peptide antibiotics.
Chemistry & biology 2000;7(12):931-42.
2000: Zamble D B; McClure C P; Penner-Hahn J E; Walsh C T
The McbB component of microcin B17 synthetase is a zinc metalloprotein.
Biochemistry 2000;39(51):16190-9.
2000: Keating T A; Marshall C G; Walsh C T
Reconstitution and characterization of the Vibrio cholerae vibriobactin synthetase from VibB, VibE, VibF, and VibH.
Biochemistry 2000;39(50):15522-30.
2000: Keating T A; Marshall C G; Walsh C T
Vibriobactin biosynthesis in Vibrio cholerae: VibH is an amide synthase homologous to nonribosomal peptide synthetase condensation domains.
Biochemistry 2000;39(50):15513-21.
2000: Suo Z; Chen H; Walsh C T
Acyl-CoA hydrolysis by the high molecular weight protein 1 subunit of yersiniabactin synthetase: mutational evidence for a cascade of four acyl-enzyme intermediates during hydrolytic editing.
Proceedings of the National Academy of Sciences of the United States of America 2000;97(26):14188-93.
2000: Chen H; Thomas M G; Hubbard B K; Losey H C; Walsh C T; Burkart M D
Deoxysugars in glycopeptide antibiotics: enzymatic synthesis of TDP-L-epivancosamine in chloroeremomycin biosynthesis.
Proceedings of the National Academy of Sciences of the United States of America 2000;97(22):11942-7.
2000: Ehmann D E; Trauger J W; Stachelhaus T; Walsh C T
Aminoacyl-SNACs as small-molecule substrates for the condensation domains of nonribosomal peptide synthetases.
Chemistry & biology 2000;7(10):765-72.
2000: Trauger J W; Kohli R M; Mootz H D; Marahiel M A; Walsh C T
Peptide cyclization catalysed by the thioesterase domain of tyrocidine synthetase.
Nature 2000;407(6801):215-8.
2000: Healy V L; Mullins L S; Li X; Hall S E; Raushel F M; Walsh C T
D-Ala-D-X ligases: evaluation of D-alanyl phosphate intermediate by MIX, PIX and rapid quench studies.
Chemistry & biology 2000;7(7):505-14.
2000: Kuzin A P; Sun T; Jorczak-Baillass J; Healy V L; Walsh C T; Knox J R
Enzymes of vancomycin resistance: the structure of D-alanine-D-lactate ligase of naturally resistant Leuconostoc mesenteroides.
Structure (London, England : 1993) 2000;8(5):463-70.
2000: Healy V L; Lessard I A; Roper D I; Knox J R; Walsh C T
Vancomycin resistance in enterococci: reprogramming of the D-ala-D-Ala ligases in bacterial peptidoglycan biosynthesis.
Chemistry & biology 2000;7(5):R109-19.
2000: Stachelhaus T; Walsh C T
Mutational analysis of the epimerization domain in the initiation module PheATE of gramicidin S synthetase.
Biochemistry 2000;39(19):5775-87.
2000: Keating T A; Miller D A; Walsh C T
Expression, purification, and characterization of HMWP2, a 229 kDa, six domain protein subunit of Yersiniabactin synthetase.
Biochemistry 2000;39(16):4729-39.
2000: Trauger J W; Walsh C T
Heterologous expression in Escherichia coli of the first module of the nonribosomal peptide synthetase for chloroeremomycin, a vancomycin-type glycopeptide antibiotic.
Proceedings of the National Academy of Sciences of the United States of America 2000;97(7):3112-7.
2000: Keating T A; Suo Z; Ehmann D E; Walsh C T
Selectivity of the yersiniabactin synthetase adenylation domain in the two-step process of amino acid activation and transfer to a holo-carrier protein domain.
Biochemistry 2000;39(9):2297-306.
2000: Ehmann D E; Shaw-Reid C A; Losey H C; Walsh C T
The EntF and EntE adenylation domains of Escherichia coli enterobactin synthetase: sequestration and selectivity in acyl-AMP transfers to thiolation domain cosubstrates.
Proceedings of the National Academy of Sciences of the United States of America 2000;97(6):2509-14.
1999: Roy R S; Allen O; Walsh C T
Expressed protein ligation to probe regiospecificity of heterocyclization in the peptide antibiotic microcin B17.
Chemistry & biology 1999;6(11):789-99.
1999: Kelleher N L; Hendrickson C L; Walsh C T
Posttranslational heterocyclization of cysteine and serine residues in the antibiotic microcin B17: distributivity and directionality.
Biochemistry 1999;38(47):15623-30.
1999: Quadri L E; Keating T A; Patel H M; Walsh C T
Assembly of the Pseudomonas aeruginosa nonribosomal peptide siderophore pyochelin: In vitro reconstitution of aryl-4, 2-bisthiazoline synthetase activity from PchD, PchE, and PchF.
Biochemistry 1999;38(45):14941-54.
1999: Suo Z; Walsh C T; Miller D A
Tandem heterocyclization activity of the multidomain 230 kDa HMWP2 subunit of Yersinia pestis yersiniabactin synthetase: interaction of the 1-1382 and 1383-2035 fragments.
Biochemistry 1999;38(42):14023-35.
1999: Lessard I A; Healy V L; Park I S; Walsh C T
Determinants for differential effects on D-Ala-D-lactate vs D-Ala-D-Ala formation by the VanA ligase from vancomycin-resistant enterococci.
Biochemistry 1999;38(42):14006-22.
1999: Kelleher N L; Zubarev R A; Bush K; Furie B; Furie B C; McLafferty F W; Walsh C T
Localization of labile posttranslational modifications by electron capture dissociation: the case of gamma-carboxyglutamic acid.
Analytical chemistry 1999;71(19):4250-3.
1999: Keating T A; Walsh C T
Initiation, elongation, and termination strategies in polyketide and polypeptide antibiotic biosynthesis.
Current opinion in chemical biology 1999;3(5):598-606.
1999: Lessard I A; Walsh C T
VanX, a bacterial D-alanyl-D-alanine dipeptidase: resistance, immunity, or survival function?
Proceedings of the National Academy of Sciences of the United States of America 1999;96(20):11028-32.
1999: Shaw-Reid C A; Kelleher N L; Losey H C; Gehring A M; Berg C; Walsh C T
Assembly line enzymology by multimodular nonribosomal peptide synthetases: the thioesterase domain of E. coli EntF catalyzes both elongation and cyclolactonization.
Chemistry & biology 1999;6(6):385-400.
1999: Sinha Roy R; Kelleher N L; Milne J C; Walsh C T
In vivo processing and antibiotic activity of microcin B17 analogs with varying ring content and altered bisheterocyclic sites.
Chemistry & biology 1999;6(5):305-18.
1999: Ehmann D E; Gehring A M; Walsh C T
Lysine biosynthesis in Saccharomyces cerevisiae: mechanism of alpha-aminoadipate reductase (Lys2) involves posttranslational phosphopantetheinylation by Lys5.
Biochemistry 1999;38(19):6171-7.
1999: Roy R S; Gehring A M; Milne J C; Belshaw P J; Walsh C T
Thiazole and oxazole peptides: biosynthesis and molecular machinery.
Natural product reports 1999;16(2):249-63.
1999: Belshaw P J; Walsh C T; Stachelhaus T
Aminoacyl-CoAs as probes of condensation domain selectivity in nonribosomal peptide synthesis.
Science (New York, N.Y.) 1999;284(5413):486-9.
1999: Milne J C; Roy R S; Eliot A C; Kelleher N L; Wokhlu A; Nickels B; Walsh C T
Cofactor requirements and reconstitution of microcin B17 synthetase: a multienzyme complex that catalyzes the formation of oxazoles and thiazoles in the antibiotic microcin B17.
Biochemistry 1999;38(15):4768-81.
1999: Lessard I A; Walsh C T
Mutational analysis of active-site residues of the enterococcal D-ala-D-Ala dipeptidase VanX and comparison with Escherichia coli D-ala-D-Ala ligase and D-ala-D-Ala carboxypeptidase VanY.
Chemistry & biology 1999;6(3):177-87.
1998: Quadri L E; Sello J; Keating T A; Weinreb P H; Walsh C T
Identification of a Mycobacterium tuberculosis gene cluster encoding the biosynthetic enzymes for assembly of the virulence-conferring siderophore mycobactin.
Chemistry & biology 1998;5(11):631-45.
1998: Gehring A M; DeMoll E; Fetherston J D; Mori I; Mayhew G F; Blattner F R; Walsh C T; Perry R D
Iron acquisition in plague: modular logic in enzymatic biogenesis of yersiniabactin by Yersinia pestis.
Chemistry & biology 1998;5(10):573-86.
1998: Silva J C; Haldimann A; Prahalad M K; Walsh C T; Wanner B L
In vivo characterization of the type A and B vancomycin-resistant enterococci (VRE) VanRS two-component systems in Escherichia coli: a nonpathogenic model for studying the VRE signal transduction pathways.
Proceedings of the National Academy of Sciences of the United States of America 1998;95(20):11951-6.
1998: Lessard I A; Pratt S D; McCafferty D G; Bussiere D E; Hutchins C; Wanner B L; Katz L; Walsh C T
Homologs of the vancomycin resistance D-Ala-D-Ala dipeptidase VanX in Streptomyces toyocaensis, Escherichia coli and Synechocystis: attributes of catalytic efficiency, stereoselectivity and regulation with implications for function.
Chemistry & biology 1998;5(9):489-504.
1998: Milne J C; Eliot A C; Kelleher N L; Walsh C T
ATP/GTP hydrolysis is required for oxazole and thiazole biosynthesis in the peptide antibiotic microcin B17.
Biochemistry 1998;37(38):13250-61.
1998: Gehring A M; Mori I; Perry R D; Walsh C T
The nonribosomal peptide synthetase HMWP2 forms a thiazoline ring during biogenesis of yersiniabactin, an iron-chelating virulence factor of Yersinia pestis.
Biochemistry 1998;37(33):11637-50.
1998: Belshaw P J; Roy R S; Kelleher N L; Walsh C T
Kinetics and regioselectivity of peptide-to-heterocycle conversions by microcin B17 synthetase.
Chemistry & biology 1998;5(7):373-84.
1998: Ritsema T; Gehring A M; Stuitje A R; van der Drift K M; Dandal I; Lambalot R H; Walsh C T; Thomas-Oates J E; Lugtenberg B J; Spaink H P
Functional analysis of an interspecies chimera of acyl carrier proteins indicates a specialized domain for protein recognition.
Molecular & general genetics : MGG 1998;257(6):641-8.
1998: Roy R S; Kim S; Baleja J D; Walsh C T
Role of the microcin B17 propeptide in substrate recognition: solution structure and mutational analysis of McbA1-26.
Chemistry & biology 1998;5(4):217-28.
1998: Healy V L; Park I S; Walsh C T
Active-site mutants of the VanC2 D-alanyl-D-serine ligase, characteristic of one vancomycin-resistant bacterial phenotype, revert towards wild-type D-alanyl-D-alanine ligases.
Chemistry & biology 1998;5(4):197-207.
1998: Sinha Roy R; Belshaw P J; Walsh C T
Mutational analysis of posttranslational heterocycle biosynthesis in the gyrase inhibitor microcin B17: distance dependence from propeptide and tolerance for substitution in a GSCG cyclizable sequence.
Biochemistry 1998;37(12):4125-36.
1998: Gehring A M; Mori I; Walsh C T
Reconstitution and characterization of the Escherichia coli enterobactin synthetase from EntB, EntE, and EntF.
Biochemistry 1998;37(8):2648-59.
1998: Skarzynski T; Kim D H; Lees W J; Walsh C T; Duncan K
Stereochemical course of enzymatic enolpyruvyl transfer and catalytic conformation of the active site revealed by the crystal structure of the fluorinated analogue of the reaction tetrahedral intermediate bound to the active site of the C115A mutant of MurA.
Biochemistry 1998;37(8):2572-7.
1998: Quadri L E; Weinreb P H; Lei M; Nakano M M; Zuber P; Walsh C T
Characterization of Sfp, a Bacillus subtilis phosphopantetheinyl transferase for peptidyl carrier protein domains in peptide synthetases.
Biochemistry 1998;37(6):1585-95.
1998: Weinreb P H; Quadri L E; Walsh C T; Zuber P
Stoichiometry and specificity of in vitro phosphopantetheinylation and aminoacylation of the valine-activating module of surfactin synthetase.
Biochemistry 1998;37(6):1575-84.
1997: Lin C H; Kwon D S; Bollinger J M; Walsh C T
Evidence for a glutathionyl-enzyme intermediate in the amidase activity of the bifunctional glutathionylspermidine synthetase/amidase from Escherichia coli.
Biochemistry 1997;36(48):14930-8.
1997: Lin C H; Chen S; Kwon D S; Coward J K; Walsh C T
Aldehyde and phosphinate analogs of glutathione and glutathionylspermidine: potent, selective binding inhibitors of the E. coli bifunctional glutathionylspermidine synthetase/amidase.
Chemistry & biology 1997;4(11):859-66.
1997: Chen S; Lin C H; Kwon D S; Walsh C T; Coward J K
Design, synthesis, and biochemical evaluation of phosphonate and phosphonamidate analogs of glutathionylspermidine as inhibitors of glutathionylspermidine synthetase/amidase from Escherichia coli.
Journal of medicinal chemistry 1997;40(23):3842-50.
1997: Walsh C T; Gehring A M; Weinreb P H; Quadri L E; Flugel R S
Post-translational modification of polyketide and nonribosomal peptide synthases.
Current opinion in chemical biology 1997;1(3):309-15.
1997: Carreras C W; Gehring A M; Walsh C T; Khosla C
Utilization of enzymatically phosphopantetheinylated acyl carrier proteins and acetyl-acyl carrier proteins by the actinorhodin polyketide synthase.
Biochemistry 1997;36(39):11757-61.
1997: Haldimann A; Fisher S L; Daniels L L; Walsh C T; Wanner B L
Transcriptional regulation of the Enterococcus faecium BM4147 vancomycin resistance gene cluster by the VanS-VanR two-component regulatory system in Escherichia coli K-12.
Journal of bacteriology 1997;179(18):5903-13.
1997: Park I S; Lin C H; Walsh C T
Bacterial resistance to vancomycin: overproduction, purification, and characterization of VanC2 from Enterococcus casseliflavus as a D-Ala-D-Ser ligase.
Proceedings of the National Academy of Sciences of the United States of America 1997;94(19):10040-4.
1997: McCafferty D G; Lessard I A; Walsh C T
Mutational analysis of potential zinc-binding residues in the active site of the enterococcal D-Ala-D-Ala dipeptidase VanX.
Biochemistry 1997;36(34):10498-505.
1997: Gehring A M; Bradley K A; Walsh C T
Enterobactin biosynthesis in Escherichia coli: isochorismate lyase (EntB) is a bifunctional enzyme that is phosphopantetheinylated by EntD and then acylated by EntE using ATP and 2,3-dihydroxybenzoate.
Biochemistry 1997;36(28):8495-503.
1997: Li S; Furie B C; Furie B; Walsh C T
The propeptide of the vitamin K-dependent carboxylase substrate accelerates formation of the gamma-glutamyl carbanion intermediate.
Biochemistry 1997;36(21):6384-90.
1997: Park I S; Walsh C T
D-Alanyl-D-lactate and D-alanyl-D-alanine synthesis by D-alanyl-D-alanine ligase from vancomycin-resistant Leuconostoc mesenteroides. Effects of a phenylalanine 261 to tyrosine mutation.
The Journal of biological chemistry 1997;272(14):9210-4.
1997: Wang J; Walsh C T
Mechanistic studies on full length and the catalytic domain of the tandem SH2 domain-containing protein tyrosine phosphatase: analysis of phosphoenzyme levels and Vmax stimulatory effects of glycerol and of a phosphotyrosyl peptide ligand.
Biochemistry 1997;36(10):2993-9.
1997: Fan C; Park I S; Walsh C T; Knox J R
D-alanine:D-alanine ligase: phosphonate and phosphinate intermediates with wild type and the Y216F mutant.
Biochemistry 1997;36(9):2531-8.
1997: Gehring A M; Lambalot R H; Vogel K W; Drueckhammer D G; Walsh C T
Ability of Streptomyces spp. acyl carrier proteins and coenzyme A analogs to serve as substrates in vitro for E. coli holo-ACP synthase.
Chemistry & biology 1997;4(1):17-24.
1997: Benson T E; Walsh C T; Hogle J M
X-ray crystal structures of the S229A mutant and wild-type MurB in the presence of the substrate enolpyruvyl-UDP-N-acetylglucosamine at 1.8-A resolution.
Biochemistry 1997;36(4):806-11.
1997: Benson T E; Walsh C T; Massey V
Kinetic characterization of wild-type and S229A mutant MurB: evidence for the role of Ser 229 as a general acid.
Biochemistry 1997;36(4):796-805.
1997: Madison L L; Vivas E I; Li Y M; Walsh C T; Kolter R
The leader peptide is essential for the post-translational modification of the DNA-gyrase inhibitor microcin B17.
Molecular microbiology 1997;23(1):161-8.
1997: Kwon D S; Lin C H; Chen S; Coward J K; Walsh C T; Bollinger J M
Dissection of glutathionylspermidine synthetase/amidase from Escherichia coli into autonomously folding and functional synthetase and amidase domains.
The Journal of biological chemistry 1997;272(4):2429-36.
1997: Lambalot R H; Walsh C T
Holo-[acyl-carrier-protein] synthase of Escherichia coli.
Methods in enzymology 1997;279():254-62.
1996: Sagoo J K; Fruman D A; Wesselborg S; Walsh C T; Bierer B E
Competitive inhibition of calcineurin phosphatase activity by its autoinhibitory domain.
The Biochemical journal 1996;320 ( Pt 3)():879-84.
1996: Haldimann A; Prahalad M K; Fisher S L; Kim S K; Walsh C T; Wanner B L
Altered recognition mutants of the response regulator PhoB: a new genetic strategy for studying protein-protein interactions.
Proceedings of the National Academy of Sciences of the United States of America 1996;93(25):14361-6.
1996: Lambalot R H; Gehring A M; Flugel R S; Zuber P; LaCelle M; Marahiel M A; Reid R; Khosla C; Walsh C T
A new enzyme superfamily - the phosphopantetheinyl transferases.
Chemistry & biology 1996;3(11):923-36.
1996: Li Y M; Milne J C; Madison L L; Kolter R; Walsh C T
From peptide precursors to oxazole and thiazole-containing peptide antibiotics: microcin B17 synthase.
Science (New York, N.Y.) 1996;274(5290):1188-93.
1996: Park I S; Lin C H; Walsh C T
Gain of D-alanyl-D-lactate or D-lactyl-D-alanine synthetase activities in three active-site mutants of the Escherichia coli D-alanyl-D-alanine ligase B.
Biochemistry 1996;35(32):10464-71.
1996: Kharbanda S; Bharti A; Pei D; Wang J; Pandey P; Ren R; Weichselbaum R; Walsh C T; Kufe D
The stress response to ionizing radiation involoves c-Abl-dependent phosphorylation of SHPTP1.
Proceedings of the National Academy of Sciences of the United States of America 1996;93(14):6898-901.
1996: Kim D H; Lees W J; Kempsell K E; Lane W S; Duncan K; Walsh C T
Characterization of a Cys115 to Asp substitution in the Escherichia coli cell wall biosynthetic enzyme UDP-GlcNAc enolpyruvyl transferase (MurA) that confers resistance to inactivation by the antibiotic fosfomycin.
Biochemistry 1996;35(15):4923-8.
1996: Fisher S L; Kim S K; Wanner B L; Walsh C T
Kinetic comparison of the specificity of the vancomycin resistance VanSfor two response regulators, VanR and PhoB.
Biochemistry 1996;35(15):4732-40.
1996: Kim D H; Tucker-Kellogg G W; Lees W J; Walsh C T
Analysis of fluoromethyl group chirality establishes a common stereochemical course for the enolpyruvyl transfers catalyzed by EPSP synthase and UDP-GlcNAc enolpyruvyl transferase.
Biochemistry 1996;35(17):5435-40.
1996: Walsh C T; Benson T E; Kim D H; Lees W J
The versatility of phosphoenolpyruvate and its vinyl ether products in biosynthesis.
Chemistry & biology 1996;3(2):83-91.
1996: Lees W J; Benson T E; Hogle J M; Walsh C T
(E)-enolbutyryl-UDP-N-acetylglucosamine as a mechanistic probe of UDP-N-acetylenolpyruvylglucosamine reductase (MurB).
Biochemistry 1996;35(5):1342-51.
1996: Pei D; Wang J; Walsh C T
Differential functions of the two Src homology 2 domains in protein tyrosine phosphatase SH-PTP1.
Proceedings of the National Academy of Sciences of the United States of America 1996;93(3):1141-5.
1996: Walsh C T; Fisher S L; Park I S; Prahalad M; Wu Z
Bacterial resistance to vancomycin: five genes and one missing hydrogen bond tell the story.
Chemistry & biology 1996;3(1):21-8.
1996: Benson T E; Walsh C T; Hogle J M
The structure of the substrate-free form of MurB, an essential enzyme for the synthesis of bacterial cell walls.
Structure (London, England : 1993) 1996;4(1):47-54.
1996: Gehring A M; Lees W J; Mindiola D J; Walsh C T; Brown E D
Acetyltransfer precedes uridylyltransfer in the formation of UDP-N-acetylglucosamine in separable active sites of the bifunctional GlmU protein of Escherichia coli.
Biochemistry 1996;35(2):579-85.
1995: Park I; Chung J; Walsh C T; Yun Y; Strominger J L; Shin J
Phosphotyrosine-independent binding of a 62-kDa protein to the src homology 2 (SH2) domain of p56lck and its regulation by phosphorylation of Ser-59 in the lck unique N-terminal region.
Proceedings of the National Academy of Sciences of the United States of America 1995;92(26):12338-42.
1995: Wu Z; Walsh C T
Phosphinate analogs of D-, D-dipeptides: slow-binding inhibition and proteolysis protection of VanX, a D-, D-dipeptidase required for vancomycin resistance in Enterococcus faecium.
Proceedings of the National Academy of Sciences of the United States of America 1995;92(25):11603-7.
1995: Lambalot R H; Walsh C T
Cloning, overproduction, and characterization of the Escherichia coli holo-acyl carrier protein synthase.
The Journal of biological chemistry 1995;270(42):24658-61.
1995: Cole P A; Grace M R; Phillips R S; Burn P; Walsh C T
The role of the catalytic base in the protein tyrosine kinase Csk.
The Journal of biological chemistry 1995;270(38):22105-8.
1995: Fisher S L; Jiang W; Wanner B L; Walsh C T
Cross-talk between the histidine protein kinase VanS and the response regulator PhoB. Characterization and identification of a VanS domain that inhibits activation of PhoB.
The Journal of biological chemistry 1995;270(39):23143-9.
1995: Benson T E; Filman D J; Walsh C T; Hogle J M
An enzyme-substrate complex involved in bacterial cell wall biosynthesis.
Nature structural biology 1995;2(8):644-53.
1995: Brown E D; Vivas E I; Walsh C T; Kolter R
MurA (MurZ), the enzyme that catalyzes the first committed step in peptidoglycan biosynthesis, is essential in Escherichia coli.
Journal of bacteriology 1995;177(14):4194-7.
1995: Bollinger J M; Kwon D S; Huisman G W; Kolter R; Walsh C T
Glutathionylspermidine metabolism in Escherichia coli. Purification, cloning, overproduction, and characterization of a bifunctional glutathionylspermidine synthetase/amidase.
The Journal of biological chemistry 1995;270(23):14031-41.
1995: Joung I; Kim T; Stolz L A; Payne G; Winkler D G; Walsh C T; Strominger J L; Shin J
Modification of Ser59 in the unique N-terminal region of tyrosine kinase p56lck regulates specificity of its Src homology 2 domain.
Proceedings of the National Academy of Sciences of the United States of America 1995;92(13):5778-82.
1995: Shi Y; Walsh C T
Active site mapping of Escherichia coli D-Ala-D-Ala ligase by structure-based mutagenesis.
Biochemistry 1995;34(9):2768-76.
1995: Roth D A; Whirl M L; Velazquez-Estades L J; Walsh C T; Furie B; Furie B C
Mutagenesis of vitamin K-dependent carboxylase demonstrates a carboxyl terminus-mediated interaction with vitamin K hydroquinone.
The Journal of biological chemistry 1995;270(10):5305-11.
1995: Wu Z; Wright G D; Walsh C T
Overexpression, purification, and characterization of VanX, a D-, D-dipeptidase which is essential for vancomycin resistance in Enterococcus faecium BM4147.
Biochemistry 1995;34(8):2455-63.
1995: Yamada M; Kuliopulos A; Nelson N P; Roth D A; Furie B; Furie B C; Walsh C T
Localization of the factor IX propeptide binding site on recombinant vitamin K dependent carboxylase using benzoylphenylalanine photoaffinity peptide inactivators.
Biochemistry 1995;34(2):481-9.
1994: Pei D; Lorenz U; Klingmüller U; Neel B G; Walsh C T
Intramolecular regulation of protein tyrosine phosphatase SH-PTP1: a new function for Src homology 2 domains.
Biochemistry 1994;33(51):15483-93.
1994: Cole P A; Burn P; Takacs B; Walsh C T
Evaluation of the catalytic mechanism of recombinant human Csk (C-terminal Src kinase) using nucleotide analogs and viscosity effects.
The Journal of biological chemistry 1994;269(49):30880-7.
1994: Thali M; Bukovsky A; Kondo E; Rosenwirth B; Walsh C T; Sodroski J; Göttlinger H G
Functional association of cyclophilin A with HIV-1 virions.
Nature 1994;372(6504):363-5.
1994: Payne G; Stolz L A; Pei D; Band H; Shoelson S E; Walsh C T
The phosphopeptide-binding specificity of Src family SH2 domains.
Chemistry & biology 1994;1(2):99-105.
1994: Marquardt J L; Brown E D; Lane W S; Haley T M; Ichikawa Y; Wong C H; Walsh C T
Kinetics, stoichiometry, and identification of the reactive thiolate in the inactivation of UDP-GlcNAc enolpyruvoyl transferase by the antibiotic fosfomycin.
Biochemistry 1994;33(35):10646-51.
1994: Brown E D; Marquardt J L; Lee J P; Walsh C T; Anderson K S
Detection and characterization of a phospholactoyl-enzyme adduct in the reaction catalyzed by UDP-N-acetylglucosamine enolpyruvoyl transferase, MurZ.
Biochemistry 1994;33(35):10638-45.
1994: Chu Y H; Lees W J; Stassinopoulos A; Walsh C T
Using affinity capillary electrophoresis to determine binding stoichiometries of protein-ligand interactions.
Biochemistry 1994;33(35):10616-21.
1994: Kuliopulos A; Nelson N P; Yamada M; Walsh C T; Furie B; Furie B C; Roth D A
Localization of the affinity peptide-substrate inactivator site on recombinant vitamin K-dependent carboxylase.
The Journal of biological chemistry 1994;269(33):21364-70.
1994: Bennett A M; Tang T L; Sugimoto S; Walsh C T; Neel B G
Protein-tyrosine-phosphatase SHPTP2 couples platelet-derived growth factor receptor beta to Ras.
Proceedings of the National Academy of Sciences of the United States of America 1994;91(15):7335-9.
1994: Benson T E; Walsh C T; Hogle J M
Crystallization and preliminary X-ray crystallographic studies of UDP-N-acetylenolpyruvylglucosamine reductase.
Protein science : a publication of the Protein Society 1994;3(7):1125-7.
1994: Fejzo J; Etzkorn F A; Clubb R T; Shi Y; Walsh C T; Wagner G
The mutant Escherichia coli F112W cyclophilin binds cyclosporin A in nearly identical conformation as human cyclophilin.
Biochemistry 1994;33(19):5711-20.
1994: Sugimoto S; Wandless T J; Shoelson S E; Neel B G; Walsh C T
Activation of the SH2-containing protein tyrosine phosphatase, SH-PTP2, by phosphotyrosine-containing peptides derived from insulin receptor substrate-1.
The Journal of biological chemistry 1994;269(18):13614-22.
1994: Holman T R; Wu Z; Wanner B L; Walsh C T
Identification of the DNA-binding site for the phosphorylated VanR protein required for vancomycin resistance in Enterococcus faecium.
Biochemistry 1994;33(15):4625-31.
1994: Comess K M; Shewchuk L M; Ivanetich K; Walsh C T
Construction of a synthetic gene for the metalloregulatory protein MerR and analysis of regionally mutated proteins for transcriptional regulation.
Biochemistry 1994;33(14):4175-86.
1994: Price E R; Jin M; Lim D; Pati S; Walsh C T; McKeon F D
Cyclophilin B trafficking through the secretory pathway is altered by binding of cyclosporin A.
Proceedings of the National Academy of Sciences of the United States of America 1994;91(9):3931-5.
1994: Shi Y; Brown E D; Walsh C T
Expression of recombinant human casein kinase II and recombinant heat shock protein 90 in Escherichia coli and characterization of their interactions.
Proceedings of the National Academy of Sciences of the United States of America 1994;91(7):2767-71.
1994: Clubb R T; Ferguson S B; Walsh C T; Wagner G
Three-dimensional solution structure of Escherichia coli periplasmic cyclophilin.
Biochemistry 1994;33(10):2761-72.
1994: Nadeau K; Nadler S G; Saulnier M; Tepper M A; Walsh C T
Quantitation of the interaction of the immunosuppressant deoxyspergualin and analogs with Hsc70 and Hsp90.
Biochemistry 1994;33(9):2561-7.
1994: Etzkorn F A; Chang Z Y; Stolz L A; Walsh C T
Cyclophilin residues that affect noncompetitive inhibition of the protein serine phosphatase activity of calcineurin by the cyclophilin.cyclosporin A complex.
Biochemistry 1994;33(9):2380-8.
1994: Lorenz U; Ravichandran K S; Pei D; Walsh C T; Burakoff S J; Neel B G
Lck-dependent tyrosyl phosphorylation of the phosphotyrosine phosphatase SH-PTP1 in murine T cells.
Molecular and cellular biology 1994;14(3):1824-34.
1994: Olson C L; Nadeau K C; Sullivan M A; Winquist A G; Donelson J E; Walsh C T; Engman D M
Molecular and biochemical comparison of the 70-kDa heat shock proteins of Trypanosoma cruzi.
The Journal of biological chemistry 1994;269(5):3868-74.
1994: Ke H; Mayrose D; Belshaw P J; Alberg D G; Schreiber S L; Chang Z Y; Etzkorn F A; Ho S; Walsh C T
Crystal structures of cyclophilin A complexed with cyclosporin A and N-methyl-4-[(E)-2-butenyl]-4,4-dimethylthreonine cyclosporin A.
Structure (London, England : 1993) 1994;2(1):33-44.
1993: Wright G D; Walsh C T
Identification of a common protease-sensitive region in D-alanyl-D-alanine and D-alanyl-D-lactate ligases and photoaffinity labeling with 8-azido ATP.
Protein science : a publication of the Protein Society 1993;2(10):1765-9.
1993: Sugimoto S; Lechleider R J; Shoelson S E; Neel B G; Walsh C T
Expression, purification, and characterization of SH2-containing protein tyrosine phosphatase, SH-PTP2.
The Journal of biological chemistry 1993;268(30):22771-6.
1993: Lechleider R J; Sugimoto S; Bennett A M; Kashishian A S; Cooper J A; Shoelson S E; Walsh C T; Neel B G
Activation of the SH2-containing phosphotyrosine phosphatase SH-PTP2 by its binding site, phosphotyrosine 1009, on the human platelet-derived growth factor receptor.
The Journal of biological chemistry 1993;268(29):21478-81.
1993: Roth D A; Rehemtulla A; Kaufman R J; Walsh C T; Furie B; Furie B C
Expression of bovine vitamin K-dependent carboxylase activity in baculovirus-infected insect cells.
Proceedings of the National Academy of Sciences of the United States of America 1993;90(18):8372-6.
1993: Walsh C T
Vancomycin resistance: decoding the molecular logic.
Science (New York, N.Y.) 1993;261(5119):308-9.
1993: Clubb R T; Thanabal V; Fejzo J; Ferguson S B; Zydowsky L; Baker C H; Walsh C T; Wagner G
Secondary structure and backbone resonance assignments of the periplasmic cyclophilin type peptidyl-prolyl isomerase from Escherichia coli.
Biochemistry 1993;32(25):6391-401.
1993: Winkler D G; Park I; Kim T; Payne N S; Walsh C T; Strominger J L; Shin J
Phosphorylation of Ser-42 and Ser-59 in the N-terminal region of the tyrosine kinase p56lck.
Proceedings of the National Academy of Sciences of the United States of America 1993;90(11):5176-80.
1993: Cho H; Krishnaraj R; Itoh M; Kitas E; Bannwarth W; Saito H; Walsh C T
Substrate specificities of catalytic fragments of protein tyrosine phosphatases (HPTP beta, LAR, and CD45) toward phosphotyrosylpeptide substrates and thiophosphotyrosylated peptides as inhibitors.
Protein science : a publication of the Protein Society 1993;2(6):977-84.
1993: Payne G; Shoelson S E; Gish G D; Pawson T; Walsh C T
Kinetics of p56lck and p60src Src homology 2 domain binding to tyrosine-phosphorylated peptides determined by a competition assay or surface plasmon resonance.
Proceedings of the National Academy of Sciences of the United States of America 1993;90(11):4902-6.
1993: Rehemtulla A; Roth D A; Wasley L C; Kuliopulos A; Walsh C T; Furie B; Furie B C; Kaufman R J
In vitro and in vivo functional characterization of bovine vitamin K-dependent gamma-carboxylase expressed in Chinese hamster ovary cells.
Proceedings of the National Academy of Sciences of the United States of America 1993;90(10):4611-5.
1993: Wright G D; Holman T R; Walsh C T
Purification and characterization of VanR and the cytosolic domain of VanS: a two-component regulatory system required for vancomycin resistance in Enterococcus faecium BM4147.
Biochemistry 1993;32(19):5057-63.
1993: Roux B; Walsh C T
p-Aminobenzoate synthesis in Escherichia coli: mutational analysis of three conserved amino acid residues of the amidotransferase PabA.
Biochemistry 1993;32(14):3763-8.
1993: Benson T E; Marquardt J L; Marquardt A C; Etzkorn F A; Walsh C T
Overexpression, purification, and mechanistic study of UDP-N-acetylenolpyruvylglucosamine reductase.
Biochemistry 1993;32(8):2024-30.
1993: Pei D; Neel B G; Walsh C T
Overexpression, purification, and characterization of SHPTP1, a Src homology 2-containing protein-tyrosine-phosphatase.
Proceedings of the National Academy of Sciences of the United States of America 1993;90(3):1092-6.
1993: Nadeau K; Das A; Walsh C T
Hsp90 chaperonins possess ATPase activity and bind heat shock transcription factors and peptidyl prolyl isomerases.
The Journal of biological chemistry 1993;268(2):1479-87.
1992: Kuliopulos A; Cieurzo C E; Furie B; Furie B C; Walsh C T
N-bromoacetyl-peptide substrate affinity labeling of vitamin K dependent carboxylase.
Biochemistry 1992;31(39):9436-44.
1992: Lee J P; Cho H; Bannwarth W; Kitas E A; Walsh C T
NMR analysis of regioselectivity in dephosphorylation of a triphosphotyrosyl dodecapeptide autophosphorylation site of the insulin receptor by a catalytic fragment of LAR phosphotyrosine phosphatase.
Protein science : a publication of the Protein Society 1992;1(10):1353-62.
1992: Marquardt J L; Siegele D A; Kolter R; Walsh C T
Cloning and sequencing of Escherichia coli murZ and purification of its product, a UDP-N-acetylglucosamine enolpyruvyl transferase.
Journal of bacteriology 1992;174(17):5748-52.
1992: Kaye R E; Fruman D A; Bierer B E; Albers M W; Zydowsky L D; Ho S I; Jin Y J; Castells M C; Schreiber S L; Walsh C T
Effects of cyclosporin A and FK506 on Fc epsilon receptor type I-initiated increases in cytokine mRNA in mouse bone marrow-derived progenitor mast cells: resistance to FK506 is associated with a deficiency in FK506-binding protein FKBP12.
Proceedings of the National Academy of Sciences of the United States of America 1992;89(18):8542-6.
1992: Zydowsky L D; Etzkorn F A; Chang H Y; Ferguson S B; Stolz L A; Ho S I; Walsh C T
Active site mutants of human cyclophilin A separate peptidyl-prolyl isomerase activity from cyclosporin A binding and calcineurin inhibition.
Protein science : a publication of the Protein Society 1992;1(9):1092-9.
1992: Roux B; Walsh C T
p-aminobenzoate synthesis in Escherichia coli: kinetic and mechanistic characterization of the amidotransferase PabA.
Biochemistry 1992;31(30):6904-10.
1992: Kuliopulos A; Hubbard B R; Lam Z; Koski I J; Furie B; Furie B C; Walsh C T
Dioxygen transfer during vitamin K dependent carboxylase catalysis.
Biochemistry 1992;31(33):7722-8.
1992: Williams S F; Palmer M A; Peoples O P; Walsh C T; Sinskey A J; Masamune S
Biosynthetic thiolase from Zoogloea ramigera. Mutagenesis of the putative active-site base Cys-378 to Ser-378 changes the partitioning of the acetyl S-enzyme intermediate.
The Journal of biological chemistry 1992;267(23):16041-3.
1992: Nadeau K; Sullivan M A; Bradley M; Engman D M; Walsh C T
83-kilodalton heat shock proteins of trypanosomes are potent peptide-stimulated ATPases.
Protein science : a publication of the Protein Society 1992;1(8):970-9.
1992: Zydowsky L D; Ho S I; Baker C H; McIntyre K; Walsh C T
Overexpression, purification, and characterization of yeast cyclophilins A and B.
Protein science : a publication of the Protein Society 1992;1(8):961-9.
1992: Walsh C T; Zydowsky L D; McKeon F D
Cyclosporin A, the cyclophilin class of peptidylprolyl isomerases, and blockade of T cell signal transduction.
The Journal of biological chemistry 1992;267(19):13115-8.
1992: Wright G D; Molinas C; Arthur M; Courvalin P; Walsh C T
Characterization of vanY, a DD-carboxypeptidase from vancomycin-resistant Enterococcus faecium BM4147.
Antimicrobial agents and chemotherapy 1992;36(7):1514-8.
1992: Bugg T D; Walsh C T
Intracellular steps of bacterial cell wall peptidoglycan biosynthesis: enzymology, antibiotics, and antibiotic resistance.
Natural product reports 1992;9(3):199-215.
1992: Arthur M; Molinas C; Bugg T D; Wright G D; Walsh C T; Courvalin P
Evidence for in vivo incorporation of D-lactate into peptidoglycan precursors of vancomycin-resistant enterococci.
Antimicrobial agents and chemotherapy 1992;36(4):867-9.
1992: Reichert J; Sakaitani M; Walsh C T
Characterization of EntF as a serine-activating enzyme.
Protein science : a publication of the Protein Society 1992;1(4):549-56.
1992: Moore M J; Miller S M; Walsh C T
C-terminal cysteines of Tn501 mercuric ion reductase.
Biochemistry 1992;31(6):1677-85.
1992: Cummings R T; Walsh C T
Interaction of Tn501 mercuric reductase and dihydroflavin adenine dinucleotide anion with metal ions: implications for the mechanism of mercuric reductase mediated Hg(II) reduction.
Biochemistry 1992;31(4):1020-30.
1992: Cho H; Ramer S E; Itoh M; Kitas E; Bannwarth W; Burn P; Saito H; Walsh C T
Catalytic domains of the LAR and CD45 protein tyrosine phosphatases from Escherichia coli expression systems: purification and characterization for specificity and mechanism.
Biochemistry 1992;31(1):133-8.
1991: Bugg T D; Wright G D; Dutka-Malen S; Arthur M; Courvalin P; Walsh C T
Molecular basis for vancomycin resistance in Enterococcus faecium BM4147: biosynthesis of a depsipeptide peptidoglycan precursor by vancomycin resistance proteins VanH and VanA.
Biochemistry 1991;30(43):10408-15.
1991: Li P M; Reichert J; Freyd G; Horvitz H R; Walsh C T
The LIM region of a presumptive Caenorhabditis elegans transcription factor is an iron-sulfur- and zinc-containing metallodomain.
Proceedings of the National Academy of Sciences of the United States of America 1991;88(20):9210-3.
1991: Ramer S E; Winkler D G; Carrera A; Roberts T M; Walsh C T
Purification and initial characterization of the lymphoid-cell protein-tyrosine kinase p56lck from a baculovirus expression system.
Proceedings of the National Academy of Sciences of the United States of America 1991;88(14):6254-8.
1991: Schiering N; Kabsch W; Moore M J; Distefano M D; Walsh C T; Pai E F
Structure of the detoxification catalyst mercuric ion reductase from Bacillus sp. strain RC607.
Nature 1991;352(6331):168-72.
1991: Bradley M; Bücheler U S; Walsh C T
Redox enzyme engineering: conversion of human glutathione reductase into a trypanothione reductase.
Biochemistry 1991;30(25):6124-7.
1991: Cho H J; Ramer S E; Itoh M; Winkler D G; Kitas E; Bannwarth W; Burn P; Saito H; Walsh C T
Purification and characterization of a soluble catalytic fragment of the human transmembrane leukocyte antigen related (LAR) protein tyrosine phosphatase from an Escherichia coli expression system.
Biochemistry 1991;30(25):6210-6.
1991: Palmer M A; Differding E; Gamboni R; Williams S F; Peoples O P; Walsh C T; Sinskey A J; Masamune S
Biosynthetic thiolase from Zoogloea ramigera. Evidence for a mechanism involving Cys-378 as the active site base.
The Journal of biological chemistry 1991;266(13):8369-75.
1991: Sullivan F X; Sobolov S B; Bradley M; Walsh C T
Mutational analysis of parasite trypanothione reductase: acquisition of glutathione reductase activity in a triple mutant.
Biochemistry 1991;30(11):2761-7.
1991: Liu J; Chen C M; Walsh C T
Human and Escherichia coli cyclophilins: sensitivity to inhibition by the immunosuppressant cyclosporin A correlates with a specific tryptophan residue.
Biochemistry 1991;30(9):2306-10.
1991: Miller S M; Massey V; Williams C H; Ballou D P; Walsh C T
Communication between the active sites in dimeric mercuric ion reductase: an alternating sites hypothesis for catalysis.
Biochemistry 1991;30(10):2600-12.
1991: Price E R; Zydowsky L D; Jin M J; Baker C H; McKeon F D; Walsh C T
Human cyclophilin B: a second cyclophilin gene encodes a peptidyl-prolyl isomerase with a signal sequence.
Proceedings of the National Academy of Sciences of the United States of America 1991;88(5):1903-7.
1991: Rusnak F; Sakaitani M; Drueckhammer D; Reichert J; Walsh C T
Biosynthesis of the Escherichia coli siderophore enterobactin: sequence of the entF gene, expression and purification of EntF, and analysis of covalent phosphopantetheine.
Biochemistry 1991;30(11):2916-27.
1991: Bugg T D; Dutka-Malen S; Arthur M; Courvalin P; Walsh C T
Identification of vancomycin resistance protein VanA as a D-alanine:D-alanine ligase of altered substrate specificity.
Biochemistry 1991;30(8):2017-21.
1991: Zawadzke L E; Bugg T D; Walsh C T
Existence of two D-alanine:D-alanine ligases in Escherichia coli: cloning and sequencing of the ddlA gene and purification and characterization of the DdlA and DdlB enzymes.
Biochemistry 1991;30(6):1673-82.
1991: Sullivan F X; Walsh C T
Cloning, sequencing, overproduction and purification of trypanothione reductase from Trypanosoma cruzi.
Molecular and biochemical parasitology 1991;44(1):145-7.
1991: Raybuck S A; Ramer S E; Abbanat D R; Peters J W; Orme-Johnson W H; Ferry J G; Walsh C T
Demonstration of carbon-carbon bond cleavage of acetyl coenzyme A by using isotopic exchange catalyzed by the CO dehydrogenase complex from acetate-grown Methanosarcina thermophila.
Journal of bacteriology 1991;173(2):929-32.
1990: Ye Q Z; Liu J; Walsh C T
p-Aminobenzoate synthesis in Escherichia coli: purification and characterization of PabB as aminodeoxychorismate synthase and enzyme X as aminodeoxychorismate lyase.
Proceedings of the National Academy of Sciences of the United States of America 1990;87(23):9391-5.
1990: Mayerl F; Piret J; Kiener A; Walsh C T; Yasui A
Functional expression of 8-hydroxy-5-deazaflavin-dependent DNA photolyase from Anacystis nidulans in Streptomyces coelicolor.
Journal of bacteriology 1990;172(10):6061-5.
1990: Alex L A; Reeve J N; Orme-Johnson W H; Walsh C T
Cloning, sequence determination, and expression of the genes encoding the subunits of the nickel-containing 8-hydroxy-5-deazaflavin reducing hydrogenase from Methanobacterium thermoautotrophicum delta H.
Biochemistry 1990;29(31):7237-44.
1990: Sakaitani M; Rusnak F; Quinn N R; Tu C; Frigo T B; Berchtold G A; Walsh C T
Mechanistic studies on trans-2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase (Ent A) in the biosynthesis of the iron chelator enterobactin.
Biochemistry 1990;29(29):6789-98.
1990: McDermott A E; Creuzet F; Griffin R G; Zawadzke L E; Ye Q Z; Walsh C T
Rotational resonance determination of the structure of an enzyme-inhibitor complex: phosphorylation of an (aminoalkyl)phosphinate inhibitor of D-alanyl-D-alanine ligase by ATP.
Biochemistry 1990;29(24):5767-75.
1990: Liu J; Walsh C T
Peptidyl-prolyl cis-trans-isomerase from Escherichia coli: a periplasmic homolog of cyclophilin that is not inhibited by cyclosporin A.
Proceedings of the National Academy of Sciences of the United States of America 1990;87(11):4028-32.
1990: Miller S M; Massey V; Ballou D; Williams C H; Distefano M D; Moore M J; Walsh C T
Use of a site-directed triple mutant to trap intermediates: demonstration that the flavin C(4a)-thiol adduct and reduced flavin are kinetically competent intermediates in mercuric ion reductase.
Biochemistry 1990;29(11):2831-41.
1990: Distefano M D; Moore M J; Walsh C T
Active site of mercuric reductase resides at the subunit interface and requires Cys135 and Cys140 from one subunit and Cys558 and Cys559 from the adjacent subunit: evidence from in vivo and in vitro heterodimer formation.
Biochemistry 1990;29(11):2703-13.
1990: Duncan K; van Heijenoort J; Walsh C T
Purification and characterization of the D-alanyl-D-alanine-adding enzyme from Escherichia coli.
Biochemistry 1990;29(9):2379-86.
1990: Liu J; Albers M W; Chen C M; Schreiber S L; Walsh C T
Cloning, expression, and purification of human cyclophilin in Escherichia coli and assessment of the catalytic role of cysteines by site-directed mutagenesis.
Proceedings of the National Academy of Sciences of the United States of America 1990;87(6):2304-8.
1990: Chen C M; Ye Q Z; Zhu Z M; Wanner B L; Walsh C T
Molecular biology of carbon-phosphorus bond cleavage. Cloning and sequencing of the phn (psiD) genes involved in alkylphosphonate uptake and C-P lyase activity in Escherichia coli B.
The Journal of biological chemistry 1990;265(8):4461-71.
1990: Helmann J D; Ballard B T; Walsh C T
The MerR metalloregulatory protein binds mercuric ion as a tricoordinate, metal-bridged dimer.
Science (New York, N.Y.) 1990;247(4945):946-8.
1990: Rusnak F; Liu J; Quinn N; Berchtold G A; Walsh C T
Subcloning of the enterobactin biosynthetic gene entB: expression, purification, characterization, and substrate specificity of isochorismatase.
Biochemistry 1990;29(6):1425-35.
1990: Liu J; Quinn N; Berchtold G A; Walsh C T
Overexpression, purification, and characterization of isochorismate synthase (EntC), the first enzyme involved in the biosynthesis of enterobactin from chorismate.
Biochemistry 1990;29(6):1417-25.
1990: Helmann J D; Shewchuk L M; Walsh C T
Regulation of gene expression by mercury.
Advances in inorganic biochemistry 1990;8():33-61.
1989: Ma H; Bloom L M; Walsh C T; Botstein D
The residual enzymatic phosphorylation activity of hexokinase II mutants is correlated with glucose repression in Saccharomyces cerevisiae.
Molecular and cellular biology 1989;9(12):5643-9.
1989: Ma H; Bloom L M; Zhu Z M; Walsh C T; Botstein D
Isolation and characterization of mutations in the HXK2 gene of Saccharomyces cerevisiae.
Molecular and cellular biology 1989;9(12):5630-42.
1989: Galakatos N G; Walsh C T
Mutations at the interdomain hinge region of the DadB alanine racemase: effects of length and conformational constraint of the linker sequence on catalytic efficiency.
Biochemistry 1989;28(20):8167-74.
1989: Moore M J; Distefano M D; Walsh C T; Schiering N; Pai E F
Purification, crystallization, and preliminary x-ray diffraction studies of the flavoenzyme mercuric ion reductase from Bacillus sp. strain RC607.
The Journal of biological chemistry 1989;264(24):14386-8.
1989: Rusnak F; Faraci W S; Walsh C T
Subcloning, expression, and purification of the enterobactin biosynthetic enzyme 2,3-dihydroxybenzoate-AMP ligase: demonstration of enzyme-bound (2,3-dihydroxybenzoyl)adenylate product.
Biochemistry 1989;28(17):6827-35.
1989: Thompson S; Mayerl F; Peoples O P; Masamune S; Sinskey A J; Walsh C T
Mechanistic studies on beta-ketoacyl thiolase from Zoogloea ramigera: identification of the active-site nucleophile as Cys89, its mutation to Ser89, and kinetic and thermodynamic characterization of wild-type and mutant enzymes.
Biochemistry 1989;28(14):5735-42.
1989: Shewchuk L M; Verdine G L; Nash H; Walsh C T
Mutagenesis of the cysteines in the metalloregulatory protein MerR indicates that a metal-bridged dimer activates transcription.
Biochemistry 1989;28(15):6140-5.
1989: Sullivan F X; Shames S L; Walsh C T
Expression of Trypanosoma congolense trypanothione reductase in Escherichia coli: overproduction, purification, and characterization.
Biochemistry 1989;28(12):4986-92.
1989: Ramer S E; Raybuck S A; Orme-Johnson W H; Walsh C T
Kinetic characterization of the [3'-32P]coenzyme A/acetyl coenzyme A exchange catalyzed by a three-subunit form of the carbon monoxide dehydrogenase/acetyl-CoA synthase from Clostridium thermoaceticum.
Biochemistry 1989;28(11):4675-80.
1989: Duncan K; Faraci W S; Matteson D S; Walsh C T
(1-Aminoethyl)boronic acid: a novel inhibitor for Bacillus stearothermophilus alanine racemase and Salmonella typhimurium D-alanine:D-alanine ligase (ADP-forming).
Biochemistry 1989;28(8):3541-9.
1989: Shewchuk L M; Verdine G L; Walsh C T
Transcriptional switching by the metalloregulatory MerR protein: initial characterization of DNA and mercury (II) binding activities.
Biochemistry 1989;28(5):2331-9.
1989: Shewchuk L M; Helmann J D; Ross W; Park S J; Summers A O; Walsh C T
Transcriptional switching by the MerR protein: activation and repression mutants implicate distinct DNA and mercury(II) binding domains.
Biochemistry 1989;28(5):2340-4.
1989: Miller S M; Moore M J; Massey V; Williams C H; Distefano M D; Ballou D P; Walsh C T
Evidence for the participation of Cys558 and Cys559 at the active site of mercuric reductase.
Biochemistry 1989;28(3):1194-205.
1989: Distefano M D; Au K G; Walsh C T
Mutagenesis of the redox-active disulfide in mercuric ion reductase: catalysis by mutant enzymes restricted to flavin redox chemistry.
Biochemistry 1989;28(3):1168-83.
1989: Walsh C T
Enzymes in the D-alanine branch of bacterial cell wall peptidoglycan assembly.
The Journal of biological chemistry 1989;264(5):2393-6.
1989: Moore M J; Walsh C T
Mutagenesis of the N- and C-terminal cysteine pairs of Tn501 mercuric ion reductase: consequences for bacterial detoxification of mercurials.
Biochemistry 1989;28(3):1183-94.
1989: Liu J; Duncan K; Walsh C T
Nucleotide sequence of a cluster of Escherichia coli enterobactin biosynthesis genes: identification of entA and purification of its product 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase.
Journal of bacteriology 1989;171(2):791-8.
1989: Faraci W S; Walsh C T
Mechanism of inactivation of alanine racemase by beta, beta, beta-trifluoroalanine.
Biochemistry 1989;28(2):431-7.
1989: Helmann J D; Wang Y; Mahler I; Walsh C T
Homologous metalloregulatory proteins from both gram-positive and gram-negative bacteria control transcription of mercury resistance operons.
Journal of bacteriology 1989;171(1):222-9.
1989: Ma H; Bloom L M; Dakin S E; Walsh C T; Botstein D
The 15 N-terminal amino acids of hexokinase II are not required for in vivo function: analysis of a truncated form of hexokinase II in Saccharomyces cerevisiae.
Proteins 1989;5(3):218-23.
1988: Raybuck S A; Bastian N R; Orme-Johnson W H; Walsh C T
Kinetic characterization of the carbon monoxide-acetyl-CoA (carbonyl group) exchange activity of the acetyl-CoA synthesizing CO dehydrogenase from Clostridium thermoaceticum.
Biochemistry 1988;27(20):7698-702.
1988: Shames S L; Kimmel B E; Peoples O P; Agabian N; Walsh C T
Trypanothione reductase of Trypanosoma congolense: gene isolation, primary sequence determination, and comparison to glutathione reductase.
Biochemistry 1988;27(14):5014-9.
1988: Copié V; Faraci W S; Walsh C T; Griffin R G
Inhibition of alanine racemase by alanine phosphonate: detection of an imine linkage to pyridoxal 5'-phosphate in the enzyme-inhibitor complex by solid-state 15N nuclear magnetic resonance.
Biochemistry 1988;27(14):4966-70.
1988: Faraci W S; Walsh C T
Racemization of alanine by the alanine racemases from Salmonella typhimurium and Bacillus stearothermophilus: energetic reaction profiles.
Biochemistry 1988;27(9):3267-76.
1988: Ploux O; Masamune S; Walsh C T
The NADPH-linked acetoacetyl-CoA reductase from Zoogloea ramigera. Characterization and mechanistic studies of the cloned enzyme over-produced in Escherichia coli.
European journal of biochemistry / FEBS 1988;174(1):177-82.
1988: Duncan K; Walsh C T
ATP-dependent inactivation and slow binding inhibition of Salmonella typhimurium D-alanine:D-alanine ligase (ADP) by (aminoalkyl)phosphinate and aminophosphonate analogues of D-alanine.
Biochemistry 1988;27(10):3709-14.
1988: Daub E; Zawadzke L E; Botstein D; Walsh C T
Isolation, cloning, and sequencing of the Salmonella typhimurium ddlA gene with purification and characterization of its product, D-alanine:D-alanine ligase (ADP forming).
Biochemistry 1988;27(10):3701-8.
1988: Chen Y C; Peoples O P; Walsh C T
Acinetobacter cyclohexanone monooxygenase: gene cloning and sequence determination.
Journal of bacteriology 1988;170(2):781-9.
1988: Walsh C T; Distefano M D; Moore M J; Shewchuk L M; Verdine G L
Molecular basis of bacterial resistance to organomercurial and inorganic mercuric salts.
The FASEB journal : official publication of the Federation of American Societies for Experimental Biology 1988;2(2):124-30.
1988: Kiener A; Orme-Johnson W H; Walsh C T
Reversible conversion of coenzyme F420 to the 8-OH-AMP and 8-OH-GMP esters, F390-A and F390-G, on oxygen exposure and reestablishment of anaerobiosis in Methanobacterium thermoautotrophicum.
Archives of microbiology 1988;150(3):249-53.
1987: Galakatos N G; Walsh C T
Specific proteolysis of native alanine racemases from Salmonella typhimurium: identification of the cleavage site and characterization of the clipped two-domain proteins.
Biochemistry 1987;26(25):8475-80.
1987: Neidhart D J; Distefano M D; Tanizawa K; Soda K; Walsh C T; Petsko G A
X-ray crystallographic studies of the alanine-specific racemase from Bacillus stearothermophilus. Overproduction, crystallization, and preliminary characterization.
The Journal of biological chemistry 1987;262(32):15323-6.
1987: Wackett L P; Honek J F; Begley T P; Wallace V; Orme-Johnson W H; Walsh C T
Substrate analogues as mechanistic probes of methyl-S-coenzyme M reductase.
Biochemistry 1987;26(19):6012-8.
1987: Walsh C T; Orme-Johnson W H
Nickel enzymes.
Biochemistry 1987;26(16):4901-6.
1987: Livingston D J; Fox J A; Orme-Johnson W H; Walsh C T
8-Hydroxy-5-deazaflavin-reducing hydrogenase from Methanobacterium thermoautotrophicum: 2. Kinetic and hydrogen-transfer studies.
Biochemistry 1987;26(14):4228-37.
1987: Fox J A; Livingston D J; Orme-Johnson W H; Walsh C T
8-Hydroxy-5-deazaflavin-reducing hydrogenase from Methanobacterium thermoautotrophicum: 1. Purification and characterization.
Biochemistry 1987;26(14):4219-27.
1987: Walsh C T; Erion M D; Walts A E; Delany J J; Berchtold G A
Chorismate aminations: partial purification of Escherichia coli PABA synthase and mechanistic comparison with anthranilate synthase.
Biochemistry 1987;26(15):4734-45.
1987: Erion M D; Walsh C T
1-Aminocyclopropanephosphonate: time-dependent inactivation of 1-aminocyclopropanecarboxylate deaminase and Bacillus stearothermophilus alanine racemase by slow dissociation behavior.
Biochemistry 1987;26(12):3417-25.
1987: Peoples O P; Masamune S; Walsh C T; Sinskey A J
Biosynthetic thiolase from Zoogloea ramigera. III. Isolation and characterization of the structural gene.
The Journal of biological chemistry 1987;262(1):97-102.
1987: Davis J T; Chen H H; Moore R; Nishitani Y; Masamune S; Sinskey A J; Walsh C T
Biosynthetic thiolase from Zoogloea ramigera. II. Inactivation with haloacetyl CoA analogs.
The Journal of biological chemistry 1987;262(1):90-6.
1987: Davis J T; Moore R N; Imperiali B; Pratt A J; Kobayashi K; Masamune S; Sinskey A J; Walsh C T; Fukui T; Tomita K
Biosynthetic thiolase from zoogloea ramigera. I. Preliminary characterization and analysis of proton transfer reaction.
The Journal of biological chemistry 1987;262(1):82-9.

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