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Antony Warren

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Omid Hekmat; Shouming He; R Antony J Warren; Stephen G Withers
A mechanism-based ICAT strategy for comparing relative expression and activity levels of glycosidases in biological systems.
Omid Hekmat; Christine Florizone; Young-Wan Kim; Lindsay D Eltis; R Antony J Warren; Stephen G Withers
Specificity fingerprinting of retaining beta-1,4-glycanases in the Cellulomonas fimi secretome using two fluorescent mechanism-based probes.
Matthew E C Caines; Mark D Vaughan; Chris A Tarling; Susan M Hancock; R Antony J Warren; Stephen G Withers; Natalie C J Strynadka
Structural and mechanistic analyses of endo-glycoceramidase II, a membrane-associated family 5 glycosidase in the Apo and GM3 ganglioside-bound forms.

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All Publications

2008: Omid Hekmat; Shouming He; R Antony J Warren; Stephen G Withers
A mechanism-based ICAT strategy for comparing relative expression and activity levels of glycosidases in biological systems.
Journal of proteome research 2008;7(8):3282-92.
2007: Omid Hekmat; Christine Florizone; Young-Wan Kim; Lindsay D Eltis; R Antony J Warren; Stephen G Withers
Specificity fingerprinting of retaining beta-1,4-glycanases in the Cellulomonas fimi secretome using two fluorescent mechanism-based probes.
Chembiochem : a European journal of chemical biology 2007;8(17):2125-32.
2007: Matthew E C Caines; Mark D Vaughan; Chris A Tarling; Susan M Hancock; R Antony J Warren; Stephen G Withers; Natalie C J Strynadka
Structural and mechanistic analyses of endo-glycoceramidase II, a membrane-associated family 5 glycosidase in the Apo and GM3 ganglioside-bound forms.
The Journal of biological chemistry 2007;282(19):14300-8.
2007: Robert Gibson; Tracey M Gloster; Shirley Roberts; R Antony J Warren; Isabel Storch de Gracia; Angela García; Jose Luis Chiara; Gideon J Davies
Molecular basis for trehalase inhibition revealed by the structure of trehalase in complex with potent inhibitors.
Angewandte Chemie (International ed. in English) 2007;46(22):4115-9.
2006: Johannes Mullegger; Hong-Ming Chen; Wing Yiu Chan; Stephen P Reid; Michael Jahn; R Antony J Warren; Hamzah Mohammed Salleh; Stephen G Withers
Thermostable glycosynthases and thioglycoligases derived from Thermotoga maritima beta-glucuronidase.
Chembiochem : a European journal of chemical biology 2006;7(7):1028-30.
2006: Christoph Mayer; David J Vocadlo; Melanie Mah; Karen Rupitz; Dominik Stoll; R Antony J Warren; Stephen G Withers
Characterization of a beta-N-acetylhexosaminidase and a beta-N-acetylglucosaminidase/beta-glucosidase from Cellulomonas fimi.
The FEBS journal 2006;273(13):2929-41.
2006: Young-Wan Kim; David T Fox; Omid Hekmat; Terrence Kantner; Lawrence P McIntosh; R Antony J Warren; Stephen G Withers
Glycosynthase-based synthesis of xylo-oligosaccharides using an engineered retaining xylanase from Cellulomonas fimi.
Organic & biomolecular chemistry 2006;4(10):2025-32.
2006: Mark D Vaughan; Karl Johnson; Shawn DeFrees; Xiaoping Tang; R Antony J Warren; Stephen G Withers
Glycosynthase-mediated synthesis of glycosphingolipids.
Journal of the American Chemical Society 2006;128(19):6300-1.
2006: Johannes Mullegger; Hong Ming Chen; R Antony J Warren; Stephen G Withers
Glycosylation of a neoglycoprotein by using glycosynthase and thioglycoligase approaches: the generation of a thioglycoprotein.
Angewandte Chemie (International ed. in English) 2006;45(16):2585-8.
2006: Hamzah M Salleh; Johannes Mullegger; Stephen P Reid; Wing Y Chan; Jiyoung Hwang; R Antony J Warren; Stephen G Withers
Cloning and characterization of Thermotoga maritima beta-glucuronidase.
Carbohydrate research 2006;341(1):49-59.
2005: Emily M Kwan; Alisdair B Boraston; Bradley W McLean; Douglas G Kilburn; R Antony J Warren
N-Glycosidase-carbohydrate-binding module fusion proteins as immobilized enzymes for protein deglycosylation.
Protein engineering, design & selection : PEDS 2005;18(10):497-501.
2005: Johannes Mullegger; Michael Jahn; Hong-Ming Chen; R Antony J Warren; Stephen G Withers
Engineering of a thioglycoligase: randomized mutagenesis of the acid-base residue leads to the identification of improved catalysts.
Protein engineering, design & selection : PEDS 2005;18(1):33-40.
2004: Young-Wan Kim; Seung Seo Lee; R Antony J Warren; Stephen G Withers
Directed evolution of a glycosynthase from Agrobacterium sp. increases its catalytic activity dramatically and expands its substrate repertoire.
The Journal of biological chemistry 2004;279(41):42787-93.
2004: Michael Jahn; Hongming Chen; Johannes Mullegger; Jennifer Marles; R Antony J Warren; Stephen G Withers
Thioglycosynthases: double mutant glycosidases that serve as scaffolds for thioglycoside synthesis.
Chemical communications (Cambridge, England) 2004;(3):274-5.
2003: Michael Jahn; Dominik Stoll; R Antony J Warren; Lóránd Szabó; Pritpal Singh; Harry J Gilbert; Valérie M-A Ducros; Gideon J Davies; Stephen G Withers
Expansion of the glycosynthase repertoire to produce defined manno-oligosaccharides.
Chemical communications (Cambridge, England) 2003;(12):1327-9.
2003: David L Zechel; Stephen P Reid; Dominik Stoll; Oyekanmi Nashiru; R Antony J Warren; Stephen G Withers
Mechanism, mutagenesis, and chemical rescue of a beta-mannosidase from cellulomonas fimi.
Biochemistry 2003;42(23):7195-204.
2003: Alisdair B Boraston; Valerie Notenboom; R Antony J Warren; Douglas G Kilburn; David R Rose; Gideon Davies
Structure and ligand binding of carbohydrate-binding module CsCBM6-3 reveals similarities with fucose-specific lectins and "galactose-binding" domains.
Journal of molecular biology 2003;327(3):659-69.
2003: Alisdair B Boraston; Emily M Kwan; Patrick Chiu; R Antony J Warren; Douglas G Kilburn
Recognition and hydrolysis of noncrystalline cellulose.
The Journal of biological chemistry 2003;278(8):6120-7.
2003: Michael Jahn; Jennifer Marles; R Antony J Warren; Stephen G Withers
Thioglycoligases: mutant glycosidases for thioglycoside synthesis.
Angewandte Chemie (International ed. in English) 2003;42(3):352-4.
2003: Alisdair B Boraston; Linda E Sandercock; R Antony J Warren; Douglas G Kilburn
O-glycosylation of a recombinant carbohydrate-binding module mutant secreted by Pichia pastoris.
Journal of molecular microbiology and biotechnology 2003;5(1):29-36.
2002: Bradley W McLean; Alisdair B Boraston; Darren Brouwer; Nooshafarin Sanaie; Colin A Fyfe; R Antony J Warren; Douglas G Kilburn; Charles A Haynes
Carbohydrate-binding modules recognize fine substructures of cellulose.
The Journal of biological chemistry 2002;277(52):50245-54.
2002: Emily M Kwan; M Marta Guarna; Alisdair B Boraston; Neil Gilkes; Charles A Haynes; Douglas G Kilburn; R Antony J Warren
Self-activating factor X derivative fused to the C-terminus of a cellulose-binding module: Production and properties.
Biotechnology and bioengineering 2002;79(7):724-32.
2002: Jakob F Tolborg; Lars Petersen; Knud J Jensen; Christoph Mayer; David L Jakeman; R Antony J Warren; Stephen G Withers
Solid-phase oligosaccharide and glycopeptide synthesis using glycosynthases.
The Journal of organic chemistry 2002;67(12):4143-9.
2002: Manuela Schärpf; Gregory P Connelly; Gregory M Lee; Alisdair B Boraston; R Antony J Warren; Lawrence P McIntosh
Site-specific characterization of the association of xylooligosaccharides with the CBM13 lectin-like xylan binding domain from Streptomyces lividans xylanase 10A by NMR spectroscopy.
Biochemistry 2002;41(13):4255-63.
2002: Alisdair B Boraston; Bradley W McLean; Grace Chen; Anson Li; R Antony J Warren; Douglas G Kilburn
Co-operative binding of triplicate carbohydrate-binding modules from a thermophilic xylanase.
Molecular microbiology 2002;43(1):187-94.
2002: Alisdair B Boraston; Mazyar Ghaffari; R Antony J Warren; Douglas G Kilburn
Identification and glucan-binding properties of a new carbohydrate-binding module family.
The Biochemical journal 2002;361(Pt 1):35-40.
2001: Alisdair B Boraston; R Antony J Warren; Douglas G Kilburn
beta-1,3-Glucan binding by a thermostable carbohydrate-binding module from Thermotoga maritima.
Biochemistry 2001;40(48):14679-85.
2001: Alisdair B Boraston; R Antony J Warren; Douglas G Kilburn
Glycosylation by Pichia pastoris decreases the affinity of a family 2a carbohydrate-binding module from Cellulomonas fimi: a functional and mutational analysis.
The Biochemical journal 2001;358(Pt 2):423-30.
2001: J E Blanchard; L Gal; Shouming He; J Foisy; R Antony J Warren; Stephen G Withers
The identification of the catalytic nucleophiles of two beta-galactosidases from glycoside hydrolase family 35.
Carbohydrate research 2001;333(1):7-17.
2001: Alisdair B Boraston; A Louise Creagh; M M Alam; JM Kormos; P Tomme; Charles A Haynes; R Antony J Warren; Douglas G Kilburn
Binding specificity and thermodynamics of a family 9 carbohydrate-binding module from Thermotoga maritima xylanase 10A.
Biochemistry 2001;40(21):6240-7.
2001: David L Zechel; Stephen P Reid; O Nashiru; Christoph Mayer; Dominik Stoll; David L Jakeman; R Antony J Warren; Stephen G Withers
Enzymatic synthesis of carbon-fluorine bonds.
Journal of the American Chemical Society 2001;123(18):4350-1.
2001: Christoph Mayer; David L Jakeman; M Mah; G Karjala; L Gal; R Antony J Warren; Stephen G Withers
Directed evolution of new glycosynthases from Agrobacterium beta-glucosidase: a general screen to detect enzymes for oligosaccharide synthesis.
Chemistry & biology 2001;8(5):437-43.
2001: Ali R Esteghlalian; V Srivastava; Neil Gilkes; Douglas G Kilburn; R Antony J Warren; J N Saddle
Do cellulose binding domains increase substrate accessibility?
Applied biochemistry and biotechnology 2001;91-93():575-92.
2000: M Marta Guarna; Hélène C F Côté; Emily M Kwan; Gordon L Rintoul; B Meyhack; Jutta Heim; Ross T A MacGillivray; R Antony J Warren; Douglas G Kilburn
Factor X fusion proteins: improved production and use in the release in vitro of biologically active hirudin from an inactive alpha-factor-hirudin fusion protein.
Protein expression and purification 2000;20(2):133-41.
2000: Dominik Stoll; Shouming He; Stephen G Withers; R Antony J Warren
Identification of Glu-519 as the catalytic nucleophile in beta-mannosidase 2A from Cellulomonas fimi.
The Biochemical journal 2000;351 Pt 3():833-8.
2000: Alisdair B Boraston; Patrick Chiu; R Antony J Warren; Douglas G Kilburn
Specificity and affinity of substrate binding by a family 17 carbohydrate-binding module from Clostridium cellulovorans cellulase 5A.
Biochemistry 2000;39(36):11129-36.
2000: Dominik Stoll; Alisdair B Boraston; Henrik Stålbrand; Bradley W McLean; Douglas G Kilburn; R Antony J Warren
Mannanase Man26A from Cellulomonas fimi has a mannan-binding module.
FEMS microbiology letters 2000;183(2):265-9.
2000: Christoph Mayer; David L Zechel; Stephen P Reid; R Antony J Warren; Stephen G Withers
The E358S mutant of Agrobacterium sp. beta-glucosidase is a greatly improved glycosynthase.
FEBS letters 2000;466(1):40-4.
1999: Dominik Stoll; Henrik Stålbrand; R Antony J Warren
Mannan-degrading enzymes from Cellulomonas fimi.
Applied and environmental microbiology 1999;65(6):2598-605.
1999: James Doheny; Eric J Jervis; M Marta Guarna; R Keith Humphries; R Antony J Warren; Douglas G Kilburn
Cellulose as an inert matrix for presenting cytokines to target cells: production and properties of a stem cell factor-cellulose-binding domain fusion protein.
The Biochemical journal 1999;339 ( Pt 2)():429-34.
1998: P Tomme; Alisdair B Boraston; Bradley W McLean; JM Kormos; A Louise Creagh; K Sturch; Neil Gilkes; Charles A Haynes; R Antony J Warren; Douglas G Kilburn
Characterization and affinity applications of cellulose-binding domains.
Journal of chromatography. B, Biomedical sciences and applications 1998;715(1):283-96.
1998: Valerie Notenboom; Camelia Chiriac; Mark Nitz; David R Rose; R Antony J Warren; Stephen G Withers
Insights into transition state stabilization of the beta-1,4-glycosidase Cex by covalent intermediate accumulation in active site mutants.
Nature structural biology 1998;5(9):812-8.
1998: Henrik Stålbrand; Shawn D Mansfield; John N Saddler; Douglas G Kilburn; R Antony J Warren; Neil Gilkes
Analysis of molecular size distributions of cellulose molecules during hydrolysis of cellulose by recombinant Cellulomonas fimi beta-1,4-glucanases.
Applied and environmental microbiology 1998;64(7):2374-9.
1998: Camelia Chiriac; P Johnson; M Joshi; A MacLeod; L McIntosh; V Monem; Mark Nitz; David R Rose; D Tull; W W Wakarchuck; Q Wang; R Antony J Warren; A White; Stephen G Withers
Mechanisms of cellulases and xylanases.
Biochemical Society transactions 1998;26(2):156-60.
1998: Valerie Notenboom; Camelia Chiriac; R Antony J Warren; Stephen G Withers; David R Rose
Exploring the cellulose/xylan specificity of the beta-1,4-glycanase cex from Cellulomonas fimi through crystallography and mutation.
Biochemistry 1998;37(14):4751-8.
1998: S L Lawson; R Antony J Warren; Stephen G Withers
Mechanistic consequences of replacing the active-site nucleophile Glu-358 in Agrobacterium sp. beta-glucosidase with a cysteine residue.
The Biochemical journal 1998;330 ( Pt 1)():203-9.
1996: P Tomme; Neil Gilkes; M Marta Guarna; Charles A Haynes; D Hasenwinkle; Eric J Jervis; P Johnson; L McIntosh; R Antony J Warren; Douglas G Kilburn
Cellulose-binding domains. Versatile affinity tags for inexpensive large-scale purification, concentration, and immobilization of fusion proteins.
Annals of the New York Academy of Sciences 1996;799():418-24.
1996: M Marta Guarna; Hélène C F Côté; E A Amandoron; Ross T A MacGillivray; R Antony J Warren; Douglas G Kilburn
Engineering factor X fusions for expression in Pichia pastoris.
Annals of the New York Academy of Sciences 1996;799():397-400.
1996: A M MacLeod; D Tull; K Rupitz; R Antony J Warren; Stephen G Withers
Mechanistic consequences of mutation of active site carboxylates in a retaining beta-1,4-glycanase from Cellulomonas fimi.
Biochemistry 1996;35(40):13165-72.
1996: P Tomme; E Kwan; Neil Gilkes; Douglas G Kilburn; R Antony J Warren
Characterization of CenC, an enzyme from Cellulomonas fimi with both endo- and exoglucanase activities.
Journal of bacteriology 1996;178(14):4216-23.
1996: H G Damude; V Ferro; Stephen G Withers; R Antony J Warren
Substrate specificity of endoglucanase A from Cellulomonas fimi: fundamental differences between endoglucanases and exoglucanases from family 6.
The Biochemical journal 1996;315 ( Pt 2)():467-72.
1995: Q Wang; D Trimbur; R Graham; R Antony J Warren; Stephen G Withers
Identification of the acid/base catalyst in Agrobacterium faecalis beta-glucosidase by kinetic analysis of mutants.
Biochemistry 1995;34(44):14554-62.
1995: H Shen; Neil Gilkes; Douglas G Kilburn; R C Miller; R Antony J Warren
Cellobiohydrolase B, a second exo-cellobiohydrolase from the cellulolytic bacterium Cellulomonas fimi.
The Biochemical journal 1995;311 ( Pt 1)():67-74.
1995: R W Graham; J M Greenwood; R Antony J Warren; Douglas G Kilburn; D E Trimbur
The pTugA and pTugAS vectors for high-level expression of cloned genes in Escherichia coli.
Gene 1995;158(1):51-4.
1995: E Ong; J B Alimonti; J M Greenwood; R C Miller; R Antony J Warren; Douglas G Kilburn
Purification of human interleukin-2 using the cellulose-binding domain of a prokaryotic cellulase.
Bioseparation 1995;5(2):95-104.
1995: A Meinke; H G Damude; P Tomme; E Kwan; Douglas G Kilburn; R C Miller; R Antony J Warren; Neil Gilkes
Enhancement of the endo-beta-1,4-glucanase activity of an exocellobiohydrolase by deletion of a surface loop.
The Journal of biological chemistry 1995;270(9):4383-6.
1995: H G Damude; Stephen G Withers; Douglas G Kilburn; R C Miller; R Antony J Warren
Site-directed mutation of the putative catalytic residues of endoglucanase CenA from Cellulomonas fimi.
Biochemistry 1995;34(7):2220-4.
1995: P Tomme; R Antony J Warren; Neil Gilkes
Cellulose hydrolysis by bacteria and fungi.
Advances in microbial physiology 1995;37():1-81.
1994: N Din; H G Damude; Neil Gilkes; R C Miller; R Antony J Warren; Douglas G Kilburn
C1-Cx revisited: intramolecular synergism in a cellulase.
Proceedings of the National Academy of Sciences of the United States of America 1994;91(24):11383-7.
1994: P Lutwyche; R Rupps; J Cavanagh; R Antony J Warren; Donald E Brooks
Cloning, sequencing, and viscometric adhesion analysis of heat-resistant agglutinin 1, an integral membrane hemagglutinin from Escherichia coli O9:H10:K99.
Infection and immunity 1994;62(11):5020-6.
1994: K D Le; Neil Gilkes; Douglas G Kilburn; R C Miller; John N Saddler; R Antony J Warren
A streptavidin-cellulose-binding domain fusion protein that binds biotinylated proteins to cellulose.
Enzyme and microbial technology 1994;16(6):496-500.
1994: A M MacLeod; T Lindhorst; Stephen G Withers; R Antony J Warren
The acid/base catalyst in the exoglucanase/xylanase from Cellulomonas fimi is glutamic acid 127: evidence from detailed kinetic studies of mutants.
Biochemistry 1994;33(20):6371-6.
1994: A Meinke; Neil Gilkes; E Kwan; Douglas G Kilburn; R Antony J Warren; R C Miller
Cellobiohydrolase A (CbhA) from the cellulolytic bacterium Cellulomonas fimi is a beta-1,4-exocellobiohydrolase analogous to Trichoderma reesei CBH II.
Molecular microbiology 1994;12(3):413-22.
1994: L E Sandercock; A M MacLeod; E Ong; R Antony J Warren
Non-S-layer glycoproteins in eubacteria.
FEMS microbiology letters 1994;118(1-2):1-7.
1994: H Shen; P Tomme; A Meinke; Neil Gilkes; Douglas G Kilburn; R Antony J Warren; R C Miller
Stereochemical course of hydrolysis catalysed by Cellulomonas fimi CenE, a member of a new family of beta-1,4-glucanases.
Biochemical and biophysical research communications 1994;199(3):1223-8.
1994: N Din; I J Forsythe; Leslie D Burtnick; Neil Gilkes; R C Miller; R Antony J Warren; Douglas G Kilburn
The cellulose-binding domain of endoglucanase A (CenA) from Cellulomonas fimi: evidence for the involvement of tryptophan residues in binding.
Molecular microbiology 1994;11(4):747-55.
1994: E Ong; Douglas G Kilburn; R C Miller; R Antony J Warren
Streptomyces lividans glycosylates the linker region of a beta-1,4-glycanase from Cellulomonas fimi.
Journal of bacteriology 1994;176(4):999-1008.
1993: Neil Gilkes; Douglas G Kilburn; R C Miller; R Antony J Warren; Junji Sugiyama; Henri Chanzy; Bernard Henrissat
Visualization of the adsorption of a bacterial endo-beta-1,4-glucanase and its isolated cellulose-binding domain to crystalline cellulose.
International journal of biological macromolecules 1993;15(6):347-51.
1993: R W Graham; T Atkinson; Douglas G Kilburn; R C Miller; R Antony J Warren
Rational design and PCR-based synthesis of an artificial Schizophyllum commune xylanase gene.
Nucleic acids research 1993;21(21):4923-8.
1993: Z Assouline; H Shen; Douglas G Kilburn; R Antony J Warren
Production and properties of a factor X-cellulose-binding domain fusion protein.
Protein engineering 1993;6(7):787-92.
1993: Q Wang; D Tull; A Meinke; Neil Gilkes; R Antony J Warren; Ruedi Aebersold; Stephen G Withers
Glu280 is the nucleophile in the active site of Clostridium thermocellum CelC, a family A endo-beta-1,4-glucanase.
The Journal of biological chemistry 1993;268(19):14096-102.
1993: A Meinke; Neil Gilkes; Douglas G Kilburn; R C Miller; R Antony J Warren
Cellulose-binding polypeptides from Cellulomonas fimi: endoglucanase D (CenD), a family A beta-1,4-glucanase.
Journal of bacteriology 1993;175(7):1910-8.
1993: H G Damude; Neil Gilkes; Douglas G Kilburn; R C Miller; R Antony J Warren
Endoglucanase CasA from alkalophilic Streptomyces strain KSM-9 is a typical member of family B of beta-1,4-glucanases.
Gene 1993;123(1):105-7.
1992: A M MacLeod; Neil Gilkes; L Escote-Carlson; R Antony J Warren; Douglas G Kilburn; R C Miller
Streptomyces lividans glycosylates an exoglucanase (Cex) from Cellulomonas fimi.
Gene 1992;121(1):143-7.
1992: Stephen G Withers; K Rupitz; D Trimbur; R Antony J Warren
Mechanistic consequences of mutation of the active site nucleophile Glu 358 in Agrobacterium beta-glucosidase.
Biochemistry 1992;31(41):9979-85.
1992: A Meinke; M Schmuck; Neil Gilkes; Douglas G Kilburn; R C Miller; R Antony J Warren
The tertiary structure of endo-beta-1,4-glucanase B (CenB), a multidomain cellulase from the bacterium Cellulomonas fimi.
Glycobiology 1992;2(4):321-6.
1992: J Gebler; Neil Gilkes; Marc Claeyssens; D B Wilson; Pierre Béguin; Warren W Wakarchuk; Douglas G Kilburn; R C Miller; R Antony J Warren; Stephen G Withers
Stereoselective hydrolysis catalyzed by related beta-1,4-glucanases and beta-1,4-xylanases.
The Journal of biological chemistry 1992;267(18):12559-61.
1992: J M Greenwood; E Ong; Neil Gilkes; R Antony J Warren; R C Miller; Douglas G Kilburn
Cellulose-binding domains: potential for purification of complex proteins.
Protein engineering 1992;5(4):361-5.
1992: D E Trimbur; R Antony J Warren; Stephen G Withers
Region-directed mutagenesis of residues surrounding the active site nucleophile in beta-glucosidase from Agrobacterium faecalis.
The Journal of biological chemistry 1992;267(15):10248-51.
1992: J B Coutinho; Neil Gilkes; R Antony J Warren; Douglas G Kilburn; R C Miller
The binding of Cellulomonas fimi endoglucanase C (CenC) to cellulose and Sephadex is mediated by the N-terminal repeats.
Molecular microbiology 1992;6(9):1243-52.
1992: P B Miller; H Shen; Neil Gilkes; Douglas G Kilburn; R C Miller; A G Plaut; R Antony J Warren
Endoglucanase A from Cellulomonas fimi in which the hinge sequence of human IgA1 is substituted for the linker connecting its two domains is hydrolyzed by IgA proteases from Neisseria gonorrhoeae.
FEMS microbiology letters 1992;71(2):199-203.
1992: Neil Gilkes; Eric J Jervis; Bernard Henrissat; B Tekant; R C Miller; R Antony J Warren; Douglas G Kilburn
The adsorption of a bacterial cellulase and its two isolated domains to crystalline cellulose.
The Journal of biological chemistry 1992;267(10):6743-9.
1991: Neil Gilkes; Marc Claeyssens; Ruedi Aebersold; Bernard Henrissat; A Meinke; H D Morrison; Douglas G Kilburn; R Antony J Warren; R C Miller
Structural and functional relationships in two families of beta-1,4-glycanases.
European journal of biochemistry / FEBS 1991;202(2):367-77.
1991: A Meinke; Neil Gilkes; Douglas G Kilburn; R C Miller; R Antony J Warren
Bacterial cellulose-binding domain-like sequences in eucaryotic polypeptides.
Protein sequences & data analysis 1991;4(6):349-53.
1991: A Meinke; Neil Gilkes; Douglas G Kilburn; R C Miller; R Antony J Warren
Multiple domains in endoglucanase B (CenB) from Cellulomonas fimi: functions and relatedness to domains in other polypeptides.
Journal of bacteriology 1991;173(22):7126-35.
1991: D Tull; Stephen G Withers; Neil Gilkes; Douglas G Kilburn; R Antony J Warren; Ruedi Aebersold
Glutamic acid 274 is the nucleophile in the active site of a "retaining" exoglucanase from Cellulomonas fimi.
The Journal of biological chemistry 1991;266(24):15621-5.
1991: H Shen; M Schmuck; I Pilz; Neil Gilkes; Douglas G Kilburn; R C Miller; R Antony J Warren
Deletion of the linker connecting the catalytic and cellulose-binding domains of endoglucanase A (CenA) of Cellulomonas fimi alters its conformation and catalytic activity.
The Journal of biological chemistry 1991;266(17):11335-40.
1991: Neil Gilkes; Bernard Henrissat; Douglas G Kilburn; R C Miller; R Antony J Warren
Domains in microbial beta-1, 4-glycanases: sequence conservation, function, and enzyme families.
Microbiological reviews 1991;55(2):303-15.
1991: J B Coutinho; Bernhard Moser; Douglas G Kilburn; R Antony J Warren; R C Miller
Nucleotide sequence of the endoglucanase C gene (cenC) of Cellulomonas fimi, its high-level expression in Escherichia coli, and characterization of its products.
Molecular microbiology 1991;5(5):1221-33.
1991: A Meinke; C Braun; Neil Gilkes; Douglas G Kilburn; R C Miller; R Antony J Warren
Unusual sequence organization in CenB, an inverting endoglucanase from Cellulomonas fimi.
Journal of bacteriology 1991;173(1):308-14.
1990: I Pilz; E Schwarz; Douglas G Kilburn; R C Miller; R Antony J Warren; Neil Gilkes
The tertiary structure of a bacterial cellulase determined by small-angle X-ray-scattering analysis.
The Biochemical journal 1990;271(1):277-80.
1990: N Din; C F Beck; R C Miller; Douglas G Kilburn; R Antony J Warren
Expression of the Cellulomonas fimi cellulase genes cex and cenA from the divergent tet promoters of transposon Tn10.
Archives of microbiology 1990;153(2):129-33.
1989: Neil Gilkes; Douglas G Kilburn; R C Miller; R Antony J Warren
Structural and functional analysis of a bacterial cellulase by proteolysis.
The Journal of biological chemistry 1989;264(30):17802-8.
1989: Bernhard Moser; Neil Gilkes; Douglas G Kilburn; R Antony J Warren; R C Miller
Purification and characterization of endoglucanase C of Cellulomonas fimi, cloning of the gene, and analysis of in vivo transcripts of the gene.
Applied and environmental microbiology 1989;55(10):2480-7.
1989: J M Greenwood; Neil Gilkes; Douglas G Kilburn; R C Miller; R Antony J Warren
Fusion to an endoglucanase allows alkaline phosphatase to bind to cellulose.
FEBS letters 1989;244(1):127-31.
1988: Neil Gilkes; R Antony J Warren; R C Miller; Douglas G Kilburn
Precise excision of the cellulose binding domains from two Cellulomonas fimi cellulases by a homologous protease and the effect on catalysis.
The Journal of biological chemistry 1988;263(21):10401-7.
1988: J B Owolabi; P Beguin; Douglas G Kilburn; R C Miller; R Antony J Warren
Expression in Escherichia coli of the Cellulomonas fimi Structural Gene for Endoglucanase B.
Applied and environmental microbiology 1988;54(2):518-23.
1988: Warren W Wakarchuk; N M Greenberg; Douglas G Kilburn; R C Miller; R Antony J Warren
Structure and transcription analysis of the gene encoding a cellobiase from Agrobacterium sp. strain ATCC 21400.
Journal of bacteriology 1988;170(1):301-7.
1987: M L Langsford; Neil Gilkes; Bhagirath Singh; Bernhard Moser; R C Miller; R Antony J Warren; Douglas G Kilburn
Glycosylation of bacterial cellulases prevents proteolytic cleavage between functional domains.
FEBS letters 1987;225(1-2):163-7.
1987: N M Greenberg; R Antony J Warren; Douglas G Kilburn; R C Miller
Regulation and initiation of cenB transcripts of Cellulomonas fimi.
Journal of bacteriology 1987;169(10):4674-7.
1987: F W Paradis; R Antony J Warren; Douglas G Kilburn; R C Miller
The expression of Cellulomonas fimi cellulase genes in Brevibacterium lactofermentum.
Gene 1987;61(2):199-206.
1987: R Antony J Warren; B Gerhard; Neil Gilkes; J B Owolabi; Douglas G Kilburn; R C Miller
A bifunctional exoglucanase-endoglucanase fusion protein.
Gene 1987;61(3):421-7.
1986: R Antony J Warren; C F Beck; Neil Gilkes; Douglas G Kilburn; M L Langsford; R C Miller; G P O'Neill; M Scheufens; W K Wong
Sequence conservation and region shuffling in an endoglucanase and an exoglucanase from Cellulomonas fimi.
Proteins 1986;1(4):335-41.
1984: Neil Gilkes; M L Langsford; Douglas G Kilburn; R C Miller; R Antony J Warren
Mode of action and substrate specificities of cellulases from cloned bacterial genes.
The Journal of biological chemistry 1984;259(16):10455-9.