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Rik Wierenga

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TOP 3
Rajaram Venkatesan; Rik K Wierenga
Structure of Mycobacterial β-Oxidation Trifunctional Enzyme Reveals Its Altered Assembly and Putative Substrate Channeling Pathway.
Satyan Sharma; Rajaram Venkatesan; Prasenjit Bhaumik; Ernst Conzelmann; J Kalervo Hiltunen; André H Juffer; Werner Schmitz; Rik K Wierenga
The enolization chemistry of a thioester-dependent racemase: the 1.4 Å crystal structure of a reaction intermediate complex characterized by detailed QM/MM calculations.
Toshiyuki Fukao; Antti M Haapalainen; Yuki Hasegawa; Mari Imamura; Naomi Kuwada; Shinsuke Maruyama; Toshihiro Ohura; Mitsuo Toyoshima; Rik K Wierenga; Seiji Yamaguchi; Isao Yuasa; Naomi Kondo
Three Japanese Patients with Beta-Ketothiolase Deficiency Who Share a Mutation, c.431A>C (H144P) in ACAT1 : Subtle Abnormality in Urinary Organic Acid Analysis and Blood Acylcarnitine Analysis Using Tandem Mass Spectrometry.

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All Publications

2013: Rajaram Venkatesan; Rik K Wierenga
Structure of Mycobacterial β-Oxidation Trifunctional Enzyme Reveals Its Altered Assembly and Putative Substrate Channeling Pathway.
ACS chemical biology 2013;8(5):1063-73.
2012: Satyan Sharma; Rajaram Venkatesan; Prasenjit Bhaumik; Ernst Conzelmann; J Kalervo Hiltunen; André H Juffer; Werner Schmitz; Rik K Wierenga
The enolization chemistry of a thioester-dependent racemase: the 1.4 Å crystal structure of a reaction intermediate complex characterized by detailed QM/MM calculations.
The journal of physical chemistry. B 2012;116(11):3619-29.
2012: Toshiyuki Fukao; Antti M Haapalainen; Yuki Hasegawa; Mari Imamura; Naomi Kuwada; Shinsuke Maruyama; Toshihiro Ohura; Mitsuo Toyoshima; Rik K Wierenga; Seiji Yamaguchi; Isao Yuasa; Naomi Kondo
Three Japanese Patients with Beta-Ketothiolase Deficiency Who Share a Mutation, c.431A>C (H144P) in ACAT1 : Subtle Abnormality in Urinary Organic Acid Analysis and Blood Acylcarnitine Analysis Using Tandem Mass Spectrometry.
JIMD reports 2012;3():107-15.
2011: Muriel Mazet; Jorge Morales; Pauline Morand; Rik K Wierenga; Frédéric Bringaud; Antti M Haapalainen; Rajesh K Harijan; Tiila-Riika Kiema; Paul A M Michels
The characterization and evolutionary relationships of a trypanosomal thiolase.
International journal for parasitology 2011;41(12):1273-83.
2011: Rajaram Venkatesan; Markus Alahuhta; Petri M Pihko; Rik K Wierenga
High resolution crystal structures of triosephosphate isomerase complexed with its suicide inhibitors: the conformational flexibility of the catalytic glutamate in its closed, liganded active site.
Protein science : a publication of the Protein Society 2011;20(8):1387-97.
2011: Imre Pápai; Andrea Hamza; Petri M Pihko; Rik K Wierenga
Stereoelectronic requirements for optimal hydrogen-bond-catalyzed enolization.
Chemistry (Weinheim an der Bergstrasse, Germany) 2011;17(10):2859-66.
2010: Rik K Wierenga; Evangelia G Kapetaniou; R Venkatesan
Triosephosphate isomerase: a highly evolved biocatalyst.
Cellular and molecular life sciences : CMLS 2010;67(23):3961-82.
2010: Mikko Salin; Evangelia G Kapetaniou; Matti Vaismaa; Marja Lajunen; Marco G Casteleijn; Peter Neubauer; Laurent Salmon; Rik K Wierenga
Crystallographic binding studies with an engineered monomeric variant of triosephosphate isomerase.
Acta crystallographica. Section D, Biological crystallography 2010;66(Pt 8):934-44.
2010: Prasad Kasaragod; Rajaram Venkatesan; Tiila-Riikka Kiema; J Kalervo Hiltunen; Rik K Wierenga
Crystal structure of liganded rat peroxisomal multifunctional enzyme type 1: a flexible molecule with two interconnected active sites.
The Journal of biological chemistry 2010;285(31):24089-98.
2010: J Kalervo Hiltunen; Zhijun Chen; Antti M Haapalainen; Rik K Wierenga; Alexander J Kastaniotis
Mitochondrial fatty acid synthesis--an adopted set of enzymes making a pathway of major importance for the cellular metabolism.
Progress in lipid research 2010;49(1):27-45.
2009: Gitte Meriläinen; Visa Poikela; Petri Kursula; Rik K Wierenga
The thiolase reaction mechanism: the importance of Asn316 and His348 for stabilizing the enolate intermediate of the Claisen condensation.
Biochemistry 2009;48(46):11011-25.
2009: Zhijun Chen; Alexander J Kastaniotis; Ilkka J Miinalainen; Venkatesan Rajaram; Rik K Wierenga; J Kalervo Hiltunen
17beta-hydroxysteroid dehydrogenase type 8 and carbonyl reductase type 4 assemble as a ketoacyl reductase of human mitochondrial FAS.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2009;23(11):3682-91.
2009: M Kristian Koski; Reija Hieta; Maija Hirsilä; Anna Rönkä; Johanna Myllyharju; Rik K Wierenga
The crystal structure of an algal prolyl 4-hydroxylase complexed with a proline-rich peptide reveals a novel buried tripeptide binding motif.
The Journal of biological chemistry 2009;284(37):25290-301.
2009: Serena Donnini; Alessandra Villa; Gerrit Groenhof; Alan E Mark; Rik K Wierenga; André H Juffer
Inclusion of ionization states of ligands in affinity calculations.
Proteins 2009;76(1):138-50.
2008: Gitte Meriläinen; Werner Schmitz; Rik K Wierenga; Petri Kursula
The sulfur atoms of the substrate CoA and the catalytic cysteine are required for a productive mode of substrate binding in bacterial biosynthetic thiolase, a thioester-dependent enzyme.
The FEBS journal 2008;275(24):6136-48.
2008: Van Dat Nguyen; Katrine Wallis; Mark J Howard; Antti M Haapalainen; Kirsi E H Salo; Mirva J Saaranen; Ateesh Sidhu; Rik K Wierenga; Robert B Freedman; Lloyd W Ruddock; Richard A Williamson
Alternative conformations of the x region of human protein disulphide-isomerase modulate exposure of the substrate binding b' domain.
Journal of molecular biology 2008;383(5):1144-55.
2008: Zhi-Jun Chen; Regina Pudas; Satyan Sharma; Oliver S Smart; André H Juffer; J Kalervo Hiltunen; Rik K Wierenga; Antti M Haapalainen
Structural enzymological studies of 2-enoyl thioester reductase of the human mitochondrial FAS II pathway: new insights into its substrate recognition properties.
Journal of molecular biology 2008;379(4):830-44.
2008: Markus Alahuhta; Mikko Salin; Marco G Casteleijn; Christian Kemmer; Ibrahim El-Sayed; Koen Augustyns; Peter Neubauer; Rik K Wierenga
Structure-based protein engineering efforts with a monomeric TIM variant: the importance of a single point mutation for generating an active site with suitable binding properties.
Protein engineering, design & selection : PEDS 2008;21(4):257-66.
2008: Markus Alahuhta; Marco G Casteleijn; Peter Neubauer; Rik K Wierenga
Structural studies show that the A178L mutation in the C-terminal hinge of the catalytic loop-6 of triosephosphate isomerase (TIM) induces a closed-like conformation in dimeric and monomeric TIM.
Acta crystallographica. Section D, Biological crystallography 2008;64(Pt 2):178-88.
2007: M Kristian Koski; Reija Hieta; Claudia Böllner; Kari I Kivirikko; Johanna Myllyharju; Rik K Wierenga
The active site of an algal prolyl 4-hydroxylase has a large structural plasticity.
The Journal of biological chemistry 2007;282(51):37112-23.
2007: Antti M Haapalainen; Gitte Meriläinen; Päivi L Pirilä; Naomi Kondo; Toshiyuki Fukao; Rik K Wierenga
Crystallographic and kinetic studies of human mitochondrial acetoacetyl-CoA thiolase: the importance of potassium and chloride ions for its structure and function.
Biochemistry 2007;46(14):4305-21.
2007: Prasenjit Bhaumik; Werner Schmitz; Antti Hassinen; J Kalervo Hiltunen; Ernst Conzelmann; Rik K Wierenga
The catalysis of the 1,1-proton transfer by alpha-methyl-acyl-CoA racemase is coupled to a movement of the fatty acyl moiety over a hydrophobic, methionine-rich surface.
Journal of molecular biology 2007;367(4):1145-61.
2007: Satomi Sakurai; Toshiyuki Fukao; Antti M Haapalainen; Gaixiu Zhang; Keitaro Yamada; Franco Lilliu; Shoji Yano; Peter Robinson; Michael K Gibson; Ronald J A Wanders; Grant A Mitchell; Rik K Wierenga; Naomi Kondo
Kinetic and expression analyses of seven novel mutations in mitochondrial acetoacetyl-CoA thiolase (T2): identification of a Km mutant and an analysis of the mutational sites in the structure.
Molecular genetics and metabolism 2007;90(4):370-8.
2007: Jukka Taskinen; Daan M F van Aalten; Jens Knudsen; Rik K Wierenga
High resolution crystal structures of unliganded and liganded human liver ACBP reveal a new mode of binding for the acyl-CoA ligand.
Proteins 2007;66(1):229-38.
2006: Marco G Casteleijn; Markus Alahuhta; Katrin Groebel; Ibrahim El-Sayed; Koen Augustyns; Anne-Marie Lambeir; Peter Neubauer; Rik K Wierenga
Functional role of the conserved active site proline of triosephosphate isomerase.
Biochemistry 2006;45(51):15483-94.
2006: Serena Donnini; Gerrit Groenhof; Rik K Wierenga; André H Juffer
The planar conformation of a strained proline ring: a QM/MM study.
Proteins 2006;64(3):700-10.
2006: Jukka Taskinen; Tiila-Riikka Kiema; J Kalervo Hiltunen; Rik K Wierenga
Structural studies of MFE-1: the 1.9 A crystal structure of the dehydrogenase part of rat peroxisomal MFE-1.
Journal of molecular biology 2006;355(4):734-46.
2006: Antti M Haapalainen; Gitte Meriläinen; Rik K Wierenga
The thiolase superfamily: condensing enzymes with diverse reaction specificities.
Trends in biochemical sciences 2006;31(1):64-71.
2005: Prasenjit Bhaumik; M Kristian Koski; Tuomo Glumoff; J Kalervo Hiltunen; Rik K Wierenga
Structural biology of the thioester-dependent degradation and synthesis of fatty acids.
Current opinion in structural biology 2005;15(6):621-8.
2005: Kalle Savolainen; Prasenjit Bhaumik; Werner Schmitz; Tiina J Kotti; Ernst Conzelmann; Rik K Wierenga; J Kalervo Hiltunen
Alpha-methylacyl-CoA racemase from Mycobacterium tuberculosis. Mutational and structural characterization of the active site and the fold.
The Journal of biological chemistry 2005;280(13):12611-20.
2005: Petri Kursula; Herkko Sikkilä; Toshiyuki Fukao; Naomi Kondo; Rik K Wierenga
High resolution crystal structures of human cytosolic thiolase (CT): a comparison of the active sites of human CT, bacterial thiolase, and bacterial KAS I.
Journal of molecular biology 2005;347(1):189-201.
2004: Mira Pekkala; Reija Hieta; Ulrich Bergmann; Kari I Kivirikko; Rik K Wierenga; Johanna Myllyharju
The peptide-substrate-binding domain of collagen prolyl 4-hydroxylases is a tetratricopeptide repeat domain with functional aromatic residues.
The Journal of biological chemistry 2004;279(50):52255-61.
2004: Prasenjit Bhaumik; M Kristian Koski; Ulrich Bergmann; Rik K Wierenga
Structure determination and refinement at 2.44 A resolution of argininosuccinate lyase from Escherichia coli.
Acta crystallographica. Section D, Biological crystallography 2004;60(Pt 11):1964-70.
2004: Sanna T Partanen; Dmitry K Novikov; Alexander N Popov; Anu M Mursula; J Kalervo Hiltunen; Rik K Wierenga
The 1.3 A crystal structure of human mitochondrial Delta3-Delta2-enoyl-CoA isomerase shows a novel mode of binding for the fatty acyl group.
Journal of molecular biology 2004;342(4):1197-208.
2004: Inari Kursula; M Salin; J Sun; BV Norledge; Antti M Haapalainen; nicole Sampson; Rik K Wierenga
Understanding protein lids: structural analysis of active hinge mutants in triosephosphate isomerase.
Protein engineering, design & selection : PEDS 2004;17(4):375-82.
2004: Anu M Mursula; J Kalervo Hiltunen; Rik K Wierenga
Structural studies on delta(3)-delta(2)-enoyl-CoA isomerase: the variable mode of assembly of the trimeric disks of the crotonase superfamily.
FEBS letters 2004;557(1-3):81-7.
2003: Mira Pekkala; Reija Hieta; Petri Kursula; Kari I Kivirikko; Rik K Wierenga; Johanna Myllyharju
Crystallization of the proline-rich-peptide binding domain of human type I collagen prolyl 4-hydroxylase.
Acta crystallographica. Section D, Biological crystallography 2003;59(Pt 5):940-2.
2003: J Kalervo Hiltunen; Anu M Mursula; Hanspeter Rottensteiner; Rik K Wierenga; Alexander J Kastaniotis; Aner Gurvitz
The biochemistry of peroxisomal beta-oxidation in the yeast Saccharomyces cerevisiae.
FEMS microbiology reviews 2003;27(1):35-64.
2003: Tomi T Airenne; Juha M Torkko; Sam Van den plas; Raija Sormunen; Alexander J Kastaniotis; Rik K Wierenga; J Kalervo Hiltunen
Structure-function analysis of enoyl thioester reductase involved in mitochondrial maintenance.
Journal of molecular biology 2003;327(1):47-59.
2003: Inari Kursula; Rik K Wierenga
Crystal structure of triosephosphate isomerase complexed with 2-phosphoglycolate at 0.83-A resolution.
The Journal of biological chemistry 2003;278(11):9544-51.
2003: Prasenjit Bhaumik; Petri Kursula; Ville Ratas; Ernst Conzelmann; J Kalervo Hiltunen; Werner Schmitz; Rik K Wierenga
Crystallization and preliminary X-ray diffraction studies of an alpha-methylacyl-CoA racemase from Mycobacterium tuberculosis.
Acta crystallographica. Section D, Biological crystallography 2003;59(Pt 2):353-5.
2002: Petri Kursula; Juha Ojala; Anne-Marie Lambeir; Rik K Wierenga
The catalytic cycle of biosynthetic thiolase: a conformational journey of an acetyl group through four binding modes and two oxyanion holes.
Biochemistry 2002;41(52):15543-56.
2002: Tiila-Riikka Kiema; Jukka Taskinen; Päivi L Pirilä; Kari T Koivuranta; Rik K Wierenga; J Kalervo Hiltunen
Organization of the multifunctional enzyme type 1: interaction between N- and C-terminal domains is required for the hydratase-1/isomerase activity.
The Biochemical journal 2002;367(Pt 2):433-41.
2002: Inari Kursula; Sanna T Partanen; Anne-Marie Lambeir; Rik K Wierenga
The importance of the conserved Arg191-Asp227 salt bridge of triosephosphate isomerase for folding, stability, and catalysis.
FEBS letters 2002;518(1-3):39-42.
2002: Jukka Taskinen; Tiila-Riikka Kiema; Kari T Koivuranta; Rik K Wierenga; J Kalervo Hiltunen
Crystallization and characterization of the dehydrogenase domain from rat peroxisomal multifunctional enzyme type 1.
Acta crystallographica. Section D, Biological crystallography 2002;58(Pt 4):690-3.
2001: Antti M Haapalainen; Daan M F van Aalten; Gitte Meriläinen; J E Jalonen; Päivi L Pirilä; Rik K Wierenga; J Kalervo Hiltunen; Tuomo Glumoff
Crystal structure of the liganded SCP-2-like domain of human peroxisomal multifunctional enzyme type 2 at 1.75 A resolution.
Journal of molecular biology 2001;313(5):1127-38.
2001: Inari Kursula; Sanna T Partanen; Anne-Marie Lambeir; D M Antonov; Koen Augustyns; Rik K Wierenga
Structural determinants for ligand binding and catalysis of triosephosphate isomerase.
European journal of biochemistry / FEBS 2001;268(19):5189-96.
2001: Anu M Mursula; Daan M F van Aalten; J Kalervo Hiltunen; Rik K Wierenga
The crystal structure of delta(3)-delta(2)-enoyl-CoA isomerase.
Journal of molecular biology 2001;309(4):845-53.
2001: Rik K Wierenga
The TIM-barrel fold: a versatile framework for efficient enzymes.
FEBS letters 2001;492(3):193-8.
2001: BV Norledge; Anne-Marie Lambeir; R A Abagyan; Antje Rottmann; A M Fernandez; Vladimir V Filimonov; Martin G Peter; Rik K Wierenga
Modeling, mutagenesis, and structural studies on the fully conserved phosphate-binding loop (loop 8) of triosephosphate isomerase: toward a new substrate specificity.
Proteins 2001;42(3):383-9.
2000: Daan M F van Aalten; Concetta C DiRusso; Jens Knudsen; Rik K Wierenga
Crystal structure of FadR, a fatty acid-responsive transcription factor with a novel acyl coenzyme A-binding fold.
The EMBO journal 2000;19(19):5167-77.
2000: Anu M Mursula; Daan M F van Aalten; Yorgo Modis; J Kalervo Hiltunen; Rik K Wierenga
Crystallization and X-ray diffraction analysis of peroxisomal Delta3-Delta2-enoyl-CoA isomerase from Saccharomyces cerevisiae.
Acta crystallographica. Section D, Biological crystallography 2000;56(Pt 8):1020-3.
2000: Daan M F van Aalten; Bjørnar Synstad; May-Bente Brurberg; Edward Hough; B W Riise; Vincent G H Eijsink; Rik K Wierenga
Structure of a two-domain chitotriosidase from Serratia marcescens at 1.9-A resolution.
Proceedings of the National Academy of Sciences of the United States of America 2000;97(11):5842-7.
2000: Anne-Marie Lambeir; Jan Backmann; J Ruiz-Sanz; Vladimir V Filimonov; Jens Nielsen; Inari Kursula; BV Norledge; Rik K Wierenga
The ionization of a buried glutamic acid is thermodynamically linked to the stability of Leishmania mexicana triose phosphate isomerase.
European journal of biochemistry / FEBS 2000;267(9):2516-24.
2000: Yorgo Modis; Rik K Wierenga
Crystallographic analysis of the reaction pathway of Zoogloea ramigera biosynthetic thiolase.
Journal of molecular biology 2000;297(5):1171-82.
2000: Daan M F van Aalten; J Knudsen; Concetta C DiRusso; T Kokko; Rik K Wierenga
Crystallization and X-ray diffraction studies of the fatty-acid responsive transcription factor FadR from Escherichia coli.
Acta crystallographica. Section D, Biological crystallography 2000;56(Pt 4):469-71.
1999: Dominique Maes; JP Zeelen; N Thanki; N Beaucamp; Marco Antonio Alvarez; M H Thi; Jan Backmann; Joseph A Martial; Lode Wyns; Rainer Jaenicke; Rik K Wierenga
The crystal structure of triosephosphate isomerase (TIM) from Thermotoga maritima: a comparative thermostability structural analysis of ten different TIM structures.
Proteins 1999;37(3):441-53.
1999: Yorgo Modis; Rik K Wierenga
A biosynthetic thiolase in complex with a reaction intermediate: the crystal structure provides new insights into the catalytic mechanism.
Structure (London, England : 1993) 1999;7(10):1279-90.
1999: Tiila-Riikka Kiema; CK Engel; Werner Schmitz; Sirpa A Filppula; Rik K Wierenga; J Kalervo Hiltunen
Mutagenic and enzymological studies of the hydratase and isomerase activities of 2-enoyl-CoA hydratase-1.
Biochemistry 1999;38(10):2991-9.
1999: JC Williams; JP Zeelen; Gitte Neubauer; G Vriend; Jan Backmann; P A Michels; Anne-Marie Lambeir; Rik K Wierenga
Structural and mutagenesis studies of leishmania triosephosphate isomerase: a point mutation can convert a mesophilic enzyme into a superstable enzyme without losing catalytic power.
Protein engineering 1999;12(3):243-50.
1998: Yorgo Modis; Rik K Wierenga
Two crystal structures of N-acetyltransferases reveal a new fold for CoA-dependent enzymes.
Structure (London, England : 1993) 1998;6(11):1345-50.
1998: Yorgo Modis; Sirpa A Filppula; Dmitry K Novikov; BV Norledge; J Kalervo Hiltunen; Rik K Wierenga
The crystal structure of dienoyl-CoA isomerase at 1.5 A resolution reveals the importance of aspartate and glutamate sidechains for catalysis.
Structure (London, England : 1993) 1998;6(8):957-70.
1998: CK Engel; Tiila-Riikka Kiema; J Kalervo Hiltunen; Rik K Wierenga
The crystal structure of enoyl-CoA hydratase complexed with octanoyl-CoA reveals the structural adaptations required for binding of a long chain fatty acid-CoA molecule.
Journal of molecular biology 1998;275(5):847-59.
1998: Marco Antonio Alvarez; JP Zeelen; V Mainfroid; Francoise Rentier-Delrue; Joseph A Martial; Lode Wyns; Rik K Wierenga; Dominique Maes
Triose-phosphate isomerase (TIM) of the psychrophilic bacterium Vibrio marinus. Kinetic and structural properties.
The Journal of biological chemistry 1998;273(4):2199-206.
1997: JC Williams; A Weijland; S Gonfloni; A Thompson; Sara A Courtneidge; Giulio Superti-Furga; Rik K Wierenga
The 2.35 A crystal structure of the inactivated form of chicken Src: a dynamic molecule with multiple regulatory interactions.
Journal of molecular biology 1997;274(5):757-75.
1997: S Gonfloni; JC Williams; K Hattula; A Weijland; Rik K Wierenga; Giulio Superti-Furga
The role of the linker between the SH2 domain and catalytic domain in the regulation and function of Src.
The EMBO journal 1997;16(24):7261-71.
1997: M Mathieu; Yorgo Modis; JP Zeelen; CK Engel; R A Abagyan; A Ahlberg; B Rasmussen; V S Lamzin; W H Kunau; Rik K Wierenga
The 1.8 A crystal structure of the dimeric peroxisomal 3-ketoacyl-CoA thiolase of Saccharomyces cerevisiae: implications for substrate binding and reaction mechanism.
Journal of molecular biology 1997;273(3):714-28.
1997: W Schliebs; N Thanki; Rainer Jaenicke; Rik K Wierenga
A double mutation at the tip of the dimer interface loop of triosephosphate isomerase generates active monomers with reduced stability.
Biochemistry 1997;36(32):9655-62.
1997: A Weijland; JC Williams; Gitte Neubauer; Sara A Courtneidge; Rik K Wierenga; Giulio Superti-Furga
Src regulated by C-terminal phosphorylation is monomeric.
Proceedings of the National Academy of Sciences of the United States of America 1997;94(8):3590-5.
1997: N Thanki; JP Zeelen; M Mathieu; Rainer Jaenicke; R A Abagyan; Rik K Wierenga; W Schliebs
Protein engineering with monomeric triosephosphate isomerase (monoTIM): the modelling and structure verification of a seven-residue loop.
Protein engineering 1997;10(2):159-67.
1996: C Engel; Rik K Wierenga
The diverse world of coenzyme A binding proteins.
Current opinion in structural biology 1996;6(6):790-7.
1996: C K Engel; M Mathieu; JP Zeelen; J Kalervo Hiltunen; Rik K Wierenga
Crystal structure of enoyl-coenzyme A (CoA) hydratase at 2.5 angstroms resolution: a spiral fold defines the CoA-binding pocket.
The EMBO journal 1996;15(19):5135-45.
1996: A Weijland; Gitte Neubauer; Sara A Courtneidge; Matthias Mann; Rik K Wierenga; Giulio Superti-Furga
The purification and characterization of the catalytic domain of Src expressed in Schizosaccharomyces pombe. Comparison of unphosphorylated and tyrosine phosphorylated species.
European journal of biochemistry / FEBS 1996;240(3):756-64.
1996: W Schliebs; N Thanki; Ramon Eritja; Rik K Wierenga
Active site properties of monomeric triosephosphate isomerase (monoTIM) as deduced from mutational and structural studies.
Protein science : a publication of the Protein Society 1996;5(2):229-39.
1995: T V Borchert; K V Kishan; JP Zeelen; W Schliebs; N Thanki; Ruben Abagyan; Rainer Jaenicke; Rik K Wierenga
Three new crystal structures of point mutation variants of monoTIM: conformational flexibility of loop-1, loop-4 and loop-8.
Structure (London, England : 1993) 1995;3(7):669-79.
1995: T V Borchert; JP Zeelen; W Schliebs; Mia callens; W Minke; Rainer Jaenicke; Rik K Wierenga
An interface point-mutation variant of triosephosphate isomerase is compactly folded and monomeric at low protein concentrations.
FEBS letters 1995;367(3):315-8.
1994: M Mathieu; JP Zeelen; R A Pauptit; Ralf Erdmann; Wolf-H Kunau; Rik K Wierenga
The 2.8 A crystal structure of peroxisomal 3-ketoacyl-CoA thiolase of Saccharomyces cerevisiae: a five-layered alpha beta alpha beta alpha structure constructed from two core domains of identical topology.
Structure (London, England : 1993) 1994;2(9):797-808.
1994: R Kishan; JP Zeelen; M E Noble; T V Borchert; V Mainfroid; K Goraj; Joseph A Martial; Rik K Wierenga
Modular mutagenesis of a TIM-barrel enzyme: the crystal structure of a chimeric E. coli TIM having the eighth beta alpha-unit replaced by the equivalent unit of chicken TIM.
Protein engineering 1994;7(8):945-51.
1994: JP Zeelen; J Kalervo Hiltunen; T A Ceska; Rik K Wierenga
Crystallization experiments with 2-enoyl-CoA hydratase, using an automated 'fast-screening' crystallization protocol.
Acta crystallographica. Section D, Biological crystallography 1994;50(Pt 4):443-7.
1994: K V Kishan; JP Zeelen; M E Noble; T V Borchert; Rik K Wierenga
Comparison of the structures and the crystal contacts of trypanosomal triosephosphate isomerase in four different crystal forms.
Protein science : a publication of the Protein Society 1994;3(5):779-87.
1994: T V Borchert; M Mathieu; JP Zeelen; S A Courtneidge; Rik K Wierenga
The crystal structure of human CskSH3: structural diversity near the RT-Src and n-Src loop.
FEBS letters 1994;341(1):79-85.
1994: L Kohl; Mia callens; Rik K Wierenga; Fred R Opperdoes; Paul A M Michels
Triose-phosphate isomerase of Leishmania mexicana mexicana. Cloning and characterization of the gene, overexpression in Escherichia coli and analysis of the protein.
European journal of biochemistry / FEBS 1994;220(2):331-8.
1994: T V Borchert; Ruben Abagyan; Rainer Jaenicke; Rik K Wierenga
Design, creation, and characterization of a stable, monomeric triosephosphate isomerase.
Proceedings of the National Academy of Sciences of the United States of America 1994;91(4):1515-8.
1993: T V Borchert; Ruben Abagyan; K V Kishan; JP Zeelen; Rik K Wierenga
The crystal structure of an engineered monomeric triosephosphate isomerase, monoTIM: the correct modelling of an eight-residue loop.
Structure (London, England : 1993) 1993;1(3):205-13.
1993: V Mainfroid; K Goraj; Francoise Rentier-Delrue; A Houbrechts; A Loiseau; A C Gohimont; M E Noble; T V Borchert; Rik K Wierenga; Joseph A Martial
Replacing the (beta alpha)-unit 8 of E.coli TIM with its chicken homologue leads to a stable and active hybrid enzyme.
Protein engineering 1993;6(8):893-900.
1993: Mia callens; J Van Roy; JP Zeelen; T V Borchert; D Nalis; Rik K Wierenga; Fred R Opperdoes
Selective interaction of glycosomal enzymes from Trypanosoma brucei with hydrophobic cyclic hexapeptides.
Biochemical and biophysical research communications 1993;195(2):667-72.
1993: M E Noble; JP Zeelen; Rik K Wierenga
Structures of the "open" and "closed" state of trypanosomal triosephosphate isomerase, as observed in a new crystal form: implications for the reaction mechanism.
Proteins 1993;16(4):311-26.
1993: M E Noble; JP Zeelen; Rik K Wierenga; V Mainfroid; K Goraj; A C Gohimont; Joseph A Martial
Structure of triosephosphate isomerase from Escherichia coli determined at 2.6 A resolution.
Acta crystallographica. Section D, Biological crystallography 1993;49(Pt 4):403-17.
1993: M E Noble; A Musacchio; Matti Saraste; S A Courtneidge; Rik K Wierenga
Crystal structure of the SH3 domain in human Fyn; comparison of the three-dimensional structures of SH3 domains in tyrosine kinases and spectrin.
The EMBO journal 1993;12(7):2617-24.
1993: J van der Oost; A Musacchio; R A Pauptit; T A Ceska; Rik K Wierenga; Matti Saraste
Crystallization and preliminary X-ray analysis of the periplasmic fragment of CyoA-a subunit of the Escherichia coli cytochrome o complex.
Journal of molecular biology 1993;229(3):794-6.
1993: T V Borchert; K Pratt; JP Zeelen; Mia callens; M E Noble; Fred R Opperdoes; Paul A M Michels; Rik K Wierenga
Overexpression of trypanosomal triosephosphate isomerase in Escherichia coli and characterisation of a dimer-interface mutant.
European journal of biochemistry / FEBS 1993;211(3):703-10.
1992: A Musacchio; M Noble; R Pauptit; Rik K Wierenga; Matti Saraste
Crystal structure of a Src-homology 3 (SH3) domain.
Nature 1992;359(6398):851-5.
1992: Rik K Wierenga; T V Borchert; M E Noble
Crystallographic binding studies with triosephosphate isomerases: conformational changes induced by substrate and substrate-analogues.
FEBS letters 1992;307(1):34-9.
1992: D A Kuntz; R Osowski; M Schudok; Rik K Wierenga; K Müller; H Kessler; Fred R Opperdoes
Inhibition of triosephosphate isomerase from Trypanosoma brucei with cyclic hexapeptides.
European journal of biochemistry / FEBS 1992;207(2):441-7.
1992: Rik K Wierenga; M E Noble; R C Davenport
Comparison of the refined crystal structures of liganded and unliganded chicken, yeast and trypanosomal triosephosphate isomerase.
Journal of molecular biology 1992;224(4):1115-26.
1992: JP Zeelen; R A Pauptit; Rik K Wierenga; Wolf-H Kunau; J Kalervo Hiltunen
Crystallization and preliminary X-ray diffraction studies of mitochondrial short-chain delta 3,delta 2-enoyl-CoA isomerase from rat liver.
Journal of molecular biology 1992;224(1):273-5.
1991: M E Noble; Christophe Verlinde; H Groendijk; Kor H Kalk; Rik K Wierenga; Wim G J Hol
Crystallographic and molecular modeling studies on trypanosomal triosephosphate isomerase: a critical assessment of the predicted and observed structures of the complex with 2-phosphoglycerate.
Journal of medicinal chemistry 1991;34(9):2709-18.
1991: Rik K Wierenga; M E Noble; G Vriend; S Nauche; Wim G J Hol
Refined 1.83 A structure of trypanosomal triosephosphate isomerase crystallized in the presence of 2.4 M-ammonium sulphate. A comparison with the structure of the trypanosomal triosephosphate isomerase-glycerol-3-phosphate complex.
Journal of molecular biology 1991;220(4):995-1015.
1991: Paul A M Michels; M Marchand; L Kohl; S Allert; Rik K Wierenga; Fred R Opperdoes
The cytosolic and glycosomal isoenzymes of glyceraldehyde-3-phosphate dehydrogenase in Trypanosoma brucei have a distant evolutionary relationship.
European journal of biochemistry / FEBS 1991;198(2):421-8.
1991: Christophe Verlinde; M E Noble; Kor H Kalk; H Groendijk; Rik K Wierenga; Wim G J Hol
Anion binding at the active site of trypanosomal triosephosphate isomerase. Monohydrogen phosphate does not mimic sulphate.
European journal of biochemistry / FEBS 1991;198(1):53-7.
1991: M E Noble; Rik K Wierenga; A M Lambeir; Fred R Opperdoes; A M Thunnissen; Kor H Kalk; H Groendijk; Wim G J Hol
The adaptability of the active site of trypanosomal triosephosphate isomerase as observed in the crystal structures of three different complexes.
Proteins 1991;10(1):50-69.
1991: Rik K Wierenga; M E Noble; J P Postma; H Groendijk; Kor H Kalk; Wim G J Hol; Fred R Opperdoes
The crystal structure of the "open" and the "closed" conformation of the flexible loop of trypanosomal triosephosphate isomerase.
Proteins 1991;10(1):33-49.
1990: JP Zeelen; Rik K Wierenga; Ralf Erdmann; Wolf-H Kunau
Crystallographic studies of 3-ketoacylCoA thiolase from yeast Saccharomyces cerevisiae.
Journal of molecular biology 1990;215(2):211-3.
1989: M Marchand; U Kooystra; Rik K Wierenga; A M Lambeir; J Van Beeumen; Fred R Opperdoes; Paul A M Michels
Glucosephosphate isomerase from Trypanosoma brucei. Cloning and characterization of the gene and analysis of the enzyme.
European journal of biochemistry / FEBS 1989;184(2):455-64.
1989: J M van der Laan; H A Schreuder; M B Swarte; Rik K Wierenga; Kor H Kalk; Wim G J Hol; J Drenth
The coenzyme analogue adenosine 5-diphosphoribose displaces FAD in the active site of p-hydroxybenzoate hydroxylase. An x-ray crystallographic investigation.
Biochemistry 1989;28(18):7199-205.
1989: H A Schreuder; P A Prick; Rik K Wierenga; Gert Vriend; K S Wilson; Wim G J Hol; J Drenth
Crystal structure of the p-hydroxybenzoate hydroxylase-substrate complex refined at 1.9 A resolution. Analysis of the enzyme-substrate and enzyme-product complexes.
Journal of molecular biology 1989;208(4):679-96.
1988: Willem J H van Berkel; F Müller; Peter A Jekel; W J Weijer; HA Schreuder; Rik K Wierenga
Chemical modification of tyrosine-38 in p-hydroxybenzoate hydroxylase from Pseudomonas fluorescens by 5'-p-fluorosulfonylbenzoyladenosine: a probe for the elucidation of the NADPH binding site? Involvement in catalysis, assignment in sequence and fitting to the tertiary structure.
European journal of biochemistry / FEBS 1988;176(2):449-59.
1988: M Marchand; A Poliszczak; W C Gibson; Rik K Wierenga; Fred R Opperdoes; Paul A M Michels
Characterization of the genes for fructose-bisphosphate aldolase in Trypanosoma brucei.
Molecular and biochemical parasitology 1988;29(1):65-75.
1987: Rik K Wierenga; Kor H Kalk; Wim G J Hol
Structure determination of the glycosomal triosephosphate isomerase from Trypanosoma brucei brucei at 2.4 A resolution.
Journal of molecular biology 1987;198(1):109-21.
1987: A M Lambeir; Fred R Opperdoes; Rik K Wierenga
Kinetic properties of triose-phosphate isomerase from Trypanosoma brucei brucei. A comparison with the rabbit muscle and yeast enzymes.
European journal of biochemistry / FEBS 1987;168(1):69-74.
1987: Randy J Read; Rik K Wierenga; H Groendijk; Wim G J Hol; A Lambeir; Fred R Opperdoes
Preliminary crystallographic studies of glycosomal glyceraldehyde phosphate dehydrogenase from Trypanosoma brucei brucei.
Journal of molecular biology 1987;194(3):573-5.
1987: Rik K Wierenga; B Swinkels; Paul A M Michels; K Osinga; O Misset; J Van Beeumen; W C Gibson; J P Postma; Piet Borst; Fred R Opperdoes
Common elements on the surface of glycolytic enzymes from Trypanosoma brucei may serve as topogenic signals for import into glycosomes.
The EMBO journal 1987;6(1):215-21.
1986: Paul A M Michels; A Poliszczak; K A Osinga; O Misset; J Van Beeumen; Rik K Wierenga; Piet Borst; Fred R Opperdoes
Two tandemly linked identical genes code for the glycosomal glyceraldehyde-phosphate dehydrogenase in Trypanosoma brucei.
The EMBO journal 1986;5(5):1049-56.
1984: Rik K Wierenga; W G Hol; O Misset; Fred R Opperdoes
Preliminary crystallographic studies of triosephosphate isomerase from the blood parasite Trypanosoma brucei brucei.
Journal of molecular biology 1984;178(2):487-90.
1983: B W Dijkstra; W J Weijer; Rik K Wierenga
Polypeptide chains with similar amino acid sequences but a distinctly different conformation. Bovine and porcine phospholipase A2.
FEBS letters 1983;164(1):25-7.
1983: Rik K Wierenga; J Drenth; G E Schulz
Comparison of the three-dimensional protein and nucleotide structure of the FAD-binding domain of p-hydroxybenzoate hydroxylase with the FAD- as well as NADPH-binding domains of glutathione reductase.
Journal of molecular biology 1983;167(3):725-39.
1983: W J Weijer; J Hofsteenge; Jaap J Beintema; Rik K Wierenga; J Drenth
p-Hydroxybenzoate hydroxylase from Pseudomonas fluorescens. 2. Fitting of the amino-acid sequence to the tertiary structure.
European journal of biochemistry / FEBS 1983;133(1):109-18.
1983: Abraham Schierbeek; J M van der Laan; H Groendijk; Rik K Wierenga; J Drenth
Crystallization and preliminary X-ray investigation of lipoamide dehydrogenase from Azotobacter vinelandii.
Journal of molecular biology 1983;165(3):563-4.
1980: J Hofsteenge; J M Vereijken; W J Weijer; Jaap J Beintema; Rik K Wierenga; J Drenth
Primary and tertiary structure studies of p-hydroxybenzoate hydroxylase from Pseudomonas fluorescens. Isolation and alignment of the CNBr peptides; interactions of the protein with flavin adenine dinucleotide.
European journal of biochemistry / FEBS 1980;113(1):141-50.