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Stephen Withers

This is a preview profile on BiomedExperts - the first literature-based scientific social network. It brings the right researchers together and allows them to collaborate online. Collexis and Dell provide the BiomedExperts network of +1.5 Million pre-calculated profiles free of charge to researchers worldwide.

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TOP 3
Rao Francesco V; Rich Jamie R; Rakic Bojana; Buddai Sai; Schwartz Marc F; Johnson Karl; Bowe Caryn; Wakarchuk Warren W; Defrees Shawn; Withers Stephen G; Strynadka Natalie C J
Structural insight into mammalian sialyltransferases.
Zhang Ran; Li Chunmin; Williams Leslie K; Rempel Brian P; Brayer Gary D; Withers Stephen G
Directed "in situ" inhibitor elongation as a strategy to structurally characterize the covalent glycosyl-enzyme intermediate of human pancreatic alpha-amylase.
Kim Jin Hyo; Ryu Hyung Won; Shim Jae Hoon; Park Ki Hun; Withers Stephen G
Development of new and selective Trypanosoma cruzi trans-sialidase inhibitors from sulfonamide chalcones and their derivatives.

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All Publications

2009: Rao Francesco V; Rich Jamie R; Rakic Bojana; Buddai Sai; Schwartz Marc F; Johnson Karl; Bowe Caryn; Wakarchuk Warren W; Defrees Shawn; Withers Stephen G; Strynadka Natalie C J
Structural insight into mammalian sialyltransferases.
Nature structural & molecular biology 2009;16(11):1186-8.
2009: Zhang Ran; Li Chunmin; Williams Leslie K; Rempel Brian P; Brayer Gary D; Withers Stephen G
Directed "in situ" inhibitor elongation as a strategy to structurally characterize the covalent glycosyl-enzyme intermediate of human pancreatic alpha-amylase.
Biochemistry 2009;48(45):10752-64.
2009: Kim Jin Hyo; Ryu Hyung Won; Shim Jae Hoon; Park Ki Hun; Withers Stephen G
Development of new and selective Trypanosoma cruzi trans-sialidase inhibitors from sulfonamide chalcones and their derivatives.
Chembiochem : a European journal of chemical biology 2009;10(15):2475-9.
2009: Shaikh Fathima Aidha; Randriantsoa Mialy; Withers Stephen G
Mechanistic analysis of the blood group antigen-cleaving endo-beta-galactosidase from Clostridium perfringens.
Biochemistry 2009;48(35):8396-404.
2009: Hancock Susan M; Rich Jamie R; Caines Matthew E C; Strynadka Natalie C J; Withers Stephen G
Designer enzymes for glycosphingolipid synthesis by directed evolution.
Nature chemical biology 2009;5(7):508-14.
2009: Morley Thomas J; Willis Lisa M; Whitfield Chris; Wakarchuk Warren W; Withers Stephen G
A new sialidase mechanism: bacteriophage K1F endo-sialidase is an inverting glycosidase.
The Journal of biological chemistry 2009;284(26):17404-10.
2009: Offen Wendy A; Zechel David L; Withers Stephen G; Gilbert Harry J; Davies Gideon J
Structure of the Michaelis complex of beta-mannosidase, Man2A, provides insight into the conformational itinerary of mannoside hydrolysis.
Chemical communications (Cambridge, England) 2009;(18):2484-6.
2009: Rich Jamie R; Withers Stephen G
Emerging methods for the production of homogeneous human glycoproteins.
Nature chemical biology 2009;5(4):206-15.
2008: Steiner Andreas J; Schitter Georg; Stütz Arnold E; Wrodnigg Tanja M; Tarling Chris A; Withers Stephen G; Fantur Katrin; Mahuran Don; Paschke Eduard; Tropak Michael
1-Deoxygalactonojirimycin-lysine hybrids as potent D-galactosidase inhibitors.
Bioorganic & medicinal chemistry 2008;16(24):10216-20.
2008: Caines Matthew E C; Zhu Haizhong; Vuckovic Marija; Willis Lisa M; Withers Stephen G; Wakarchuk Warren W; Strynadka Natalie C J
The structural basis for T-antigen hydrolysis by Streptococcus pneumoniae: a target for structure-based vaccine design.
The Journal of biological chemistry 2008;283(46):31279-83.
2008: Hancock Susan M; Tarling Chris A; Withers Stephen G
High-throughput screening of cell lysates for ganglioside synthesis.
Analytical biochemistry 2008;382(1):48-54.
2008: Hekmat Omid; He Shouming; Warren R Antony J; Withers Stephen G
A mechanism-based ICAT strategy for comparing relative expression and activity levels of glycosidases in biological systems.
Journal of proteome research 2008;7(8):3282-92.
2008: Rempel Brian P; Withers Stephen G
Covalent inhibitors of glycosidases and their applications in biochemistry and biology.
Glycobiology 2008;18(8):570-86.
2008: Lee Young-Su; Lee Myoung-Hee; Lee Hee-Seob; Lee Seung-Jae; Kim Young-Wan; Zhang Ran; Withers Stephen G; Kim Kwan Soo; Lee Sung-Joon; Park Kwan-Hwa
Enzymatic synthesis of a selective inhibitor for alpha-glucosidases: alpha-acarviosinyl-(1-->9)-3-alpha-D-glucopyranosylpropen.
Journal of agricultural and food chemistry 2008;56(13):5324-30.
2008: Shaikh Fathima Aidha; Withers Stephen G
Teaching old enzymes new tricks: engineering and evolution of glycosidases and glycosyl transferases for improved glycoside synthesis.
Biochemistry and cell biology = Biochimie et biologie cellulaire 2008;86(2):169-77.
2008: Zhang Ran; McCarter John D; Braun Curtis; Yeung Wai; Brayer Gary D; Withers Stephen G
Synthesis and testing of 2-deoxy-2,2-dihaloglycosides as mechanism-based inhibitors of alpha-glycosidases.
The Journal of organic chemistry 2008;73(8):3070-7.
2008: Eltis Lindsay D; Withers Stephen G
Nature teaches but can be bettered.
Current opinion in chemical biology 2008;12(2):115-7.
2008: Maurus Robert; Begum Anjuman; Williams Leslie K; Fredriksen Jason R; Zhang Ran; Withers Stephen G; Brayer Gary D
Alternative catalytic anions differentially modulate human alpha-amylase activity and specificity.
Biochemistry 2008;47(11):3332-44.
2008: Damager Iben; Buchini Sabrina; Amaya Maria F; Buschiazzo Alejandro; Alzari Pedro; Frasch Alberto C; Watts Andrew; Withers Stephen G
Kinetic and mechanistic analysis of Trypanosoma cruzi trans-sialidase reveals a classical ping-pong mechanism with acid/base catalysis.
Biochemistry 2008;47(11):3507-12.
2008: Steiner Andreas J; Stütz Arnold E; Wrodnigg Tanja M; Tarling Chris A; Withers Stephen G; Hermetter Albin; Schmidinger Hannes
Glycosidase profiling with immobilised glycosidase-inhibiting iminoalditols--a proof-of-concept study.
Bioorganic & medicinal chemistry letters 2008;18(6):1922-5.
2008: Tarling Chris A; Woods Kate; Zhang Ran; Brastianos Harry C; Brayer Gary D; Andersen Raymond J; Withers Stephen G
The search for novel human pancreatic alpha-amylase inhibitors: high-throughput screening of terrestrial and marine natural product extracts.
Chembiochem : a European journal of chemical biology 2008;9(3):433-8.
2008: Lairson L L; Henrissat B; Davies G J; Withers S G
Glycosyltransferases: structures, functions, and mechanisms.
Annual review of biochemistry 2008;77():521-55.
2008: Buchini Sabrina; Buschiazzo Alejandro; Withers Stephen G
A new generation of specific Trypanosoma cruzi trans-sialidase inhibitors.
Angewandte Chemie (International ed. in English) 2008;47(14):2700-3.
2007: Hekmat Omid; Florizone Christine; Kim Young-Wan; Eltis Lindsay D; Warren R Antony J; Withers Stephen G
Specificity fingerprinting of retaining beta-1,4-glycanases in the Cellulomonas fimi secretome using two fluorescent mechanism-based probes.
Chembiochem : a European journal of chemical biology 2007;8(17):2125-32.
2007: Chen Hong-Ming; Withers Stephen G
Syntheses of the 3- and 4-thio analogues of 4-nitrophenyl 2-acetamido-2-deoxy-beta-D-gluco- and galactopyranoside.
Carbohydrate research 2007;342(15):2212-22.
2007: Kim Young-Wan; Chen Hong-Ming; Kim Jin Hyo; Müllegger Johannes; Mahuran Don; Withers Stephen G
Thioglycoligase-based assembly of thiodisaccharides: screening as beta-galactosidase inhibitors.
Chembiochem : a European journal of chemical biology 2007;8(13):1495-9.
2007: Steiner Andreas J; Stütz Arnold E; Tarling Chris A; Withers Stephen G; Wrodnigg Tanja M
Iminoalditol-amino acid hybrids: synthesis and evaluation as glycosidase inhibitors.
Carbohydrate research 2007;342(12-13):1850-8.
2007: Yip Vivian L Y; Thompson John; Withers Stephen G
Mechanism of GlvA from Bacillus subtilis: a detailed kinetic analysis of a 6-phospho-alpha-glucosidase from glycoside hydrolase family 4.
Biochemistry 2007;46(34):9840-52.
2007: Schubert Mario; Poon David K Y; Wicki Jacqueline; Tarling Chris A; Kwan Emily M; Nielsen Jens E; Withers Stephen G; McIntosh Lawrence P
Probing electrostatic interactions along the reaction pathway of a glycoside hydrolase: histidine characterization by NMR spectroscopy.
Biochemistry 2007;46(25):7383-95.
2007: Chiu Cecilia P C; Lairson Luke L; Gilbert Michel; Wakarchuk Warren W; Withers Stephen G; Strynadka Natalie C J
Structural analysis of the alpha-2,3-sialyltransferase Cst-I from Campylobacter jejuni in apo and substrate-analogue bound forms.
Biochemistry 2007;46(24):7196-204.
2007: Wicki Jacqueline; Schloegl Johann; Tarling Chris A; Withers Stephen G
Recruitment of both uniform and differential binding energy in enzymatic catalysis: xylanases from families 10 and 11.
Biochemistry 2007;46(23):6996-7005.
2007: Shaikh Fathima Aidha; Müllegger Johannes; He Shouming; Withers Stephen G
Identification of the catalytic nucleophile in Family 42 beta-galactosidases by intermediate trapping and peptide mapping: YesZ from Bacillus subtilis.
FEBS letters 2007;581(13):2441-6.
2007: Chen Hong-Ming; Withers Stephen G
Facile synthesis of 2,4-dinitrophenyl alpha-D-glycopyranosides as chromogenic substrates for alpha-glycosidases.
Chembiochem : a European journal of chemical biology 2007;8(7):719-22.
2007: Caines Matthew E C; Vaughan Mark D; Tarling Chris A; Hancock Susan M; Warren R Antony J; Withers Stephen G; Strynadka Natalie C J
Structural and mechanistic analyses of endo-glycoceramidase II, a membrane-associated family 5 glycosidase in the Apo and GM3 ganglioside-bound forms.
The Journal of biological chemistry 2007;282(19):14300-8.
2007: Wicki Jacqueline; Williams Spencer J; Withers Stephen G
Transition-state mimicry by glycosidase inhibitors: a critical kinetic analysis.
Journal of the American Chemical Society 2007;129(15):4530-1.
2007: Poon David K Y; Ludwiczek Martin L; Schubert Mario; Kwan Emily M; Withers Stephen G; McIntosh Lawrence P
NMR spectroscopic characterization of a beta-(1,4)-glycosidase along its reaction pathway: stabilization upon formation of the glycosyl-enzyme intermediate.
Biochemistry 2007;46(7):1759-70.
2007: Lairson Luke L; Wakarchuk Warren W; Withers Stephen G
Alternative donor substrates for inverting and retaining glycosyltransferases.
Chemical communications (Cambridge, England) 2007;(4):365-7.
2007: Poon David K Y; Withers Stephen G; McIntosh Lawrence P
Direct demonstration of the flexibility of the glycosylated proline-threonine linker in the Cellulomonas fimi Xylanase Cex through NMR spectroscopic analysis.
The Journal of biological chemistry 2007;282(3):2091-100.
2007: Tesic Milica; Wicki Jacqueline; Poon David K Y; Withers Stephen G; Douglas Donald J
Gas phase noncovalent protein complexes that retain solution binding properties: Binding of xylobiose inhibitors to the beta-1, 4 exoglucanase from cellulomonas fimi.
Journal of the American Society for Mass Spectrometry 2007;18(1):64-73.
2007: Caines Matthew E C; Hancock Susan M; Tarling Chris A; Wrodnigg Tanja M; Stick Robert V; Stütz Arnold E; Vasella Andrea; Withers Stephen G; Strynadka Natalie C J
The structural basis of glycosidase inhibition by five-membered iminocyclitols: the clan a glycoside hydrolase endoglycoceramidase as a model system.
Angewandte Chemie (International ed. in English) 2007;46(24):4474-6.
2006: Poon David K Y; Schubert Mario; Au Jason; Okon Mark; Withers Stephen G; McIntosh Lawrence P
Unambiguous determination of the ionization state of a glycoside hydrolase active site lysine by 1H-15N heteronuclear correlation spectroscopy.
Journal of the American Chemical Society 2006;128(48):15388-9.
2006: Lairson Luke L; Watts Andrew G; Wakarchuk Warren W; Withers Stephen G
Using substrate engineering to harness enzymatic promiscuity and expand biological catalysis.
Nature chemical biology 2006;2(12):724-8.
2006: Hancock Susan M; Vaughan Mark D; Withers Stephen G
Engineering of glycosidases and glycosyltransferases.
Current opinion in chemical biology 2006;10(5):509-19.
2006: Yip Vivian L Y; Withers Stephen G
Family 4 glycosidases carry out efficient hydrolysis of thioglycosides by an alpha,beta-elimination mechanism.
Angewandte Chemie (International ed. in English) 2006;45(37):6179-82.
2006: Blair David E; Hekmat Omid; Schüttelkopf Alexander W; Shrestha Binesh; Tokuyasu Ken; Withers Stephen G; van Aalten Daan M F
Structure and mechanism of chitin deacetylase from the fungal pathogen Colletotrichum lindemuthianum.
Biochemistry 2006;45(31):9416-26.
2006: Aharoni Amir; Thieme Karena; Chiu Cecilia P C; Buchini Sabrina; Lairson Luke L; Chen Hongming; Strynadka Natalie C J; Wakarchuk Warren W; Withers Stephen G
High-throughput screening methodology for the directed evolution of glycosyltransferases.
Nature methods 2006;3(8):609-14.
2006: Kim Young-Wan; Chen Hongming; Kim Jin Hyo; Withers Stephen G
Catalytic properties of a mutant beta-galactosidase from Xanthomonas manihotis engineered to synthesize galactosyl-thio-beta-1,3 and -beta-1,4-glycosides.
FEBS letters 2006;580(18):4377-81.
2006: Müllegger Johannes; Chen Hong-Ming; Chan Wing Yiu; Reid Stephen P; Jahn Michael; Warren R Antony J; Salleh Hamzah Mohammed; Withers Stephen G
Thermostable glycosynthases and thioglycoligases derived from Thermotoga maritima beta-glucuronidase.
Chembiochem : a European journal of chemical biology 2006;7(7):1028-30.
2006: Mayer Christoph; Vocadlo David J; Mah Melanie; Rupitz Karen; Stoll Dominik; Warren R A J; Withers Stephen G
Characterization of a beta-N-acetylhexosaminidase and a beta-N-acetylglucosaminidase/beta-glucosidase from Cellulomonas fimi.
The FEBS journal 2006;273(13):2929-41.
2006: Wrodnigg Tanja M; Stütz Arnold E; Tarling Chris A; Withers Stephen G
Fine tuning of beta-glucosidase inhibitory activity in the 2,5-dideoxy-2,5-imino-D-mannitol (DMDP) system.
Carbohydrate research 2006;341(10):1717-22.
2006: Gordon Roni D; Sivarajah Prashanth; Satkunarajah Malathy; Ma Dengbo; Tarling Chris A; Vizitiu Dragos; Withers Stephen G; Rini James M
X-ray crystal structures of rabbit N-acetylglucosaminyltransferase I (GnT I) in complex with donor substrate analogues.
Journal of molecular biology 2006;360(1):67-79.
2006: Lemieux M Joanne; Mark Brian L; Cherney Maia M; Withers Stephen G; Mahuran Don J; James Michael N G
Crystallographic structure of human beta-hexosaminidase A: interpretation of Tay-Sachs mutations and loss of GM2 ganglioside hydrolysis.
Journal of molecular biology 2006;359(4):913-29.
2006: Kim Young-Wan; Fox David T; Hekmat Omid; Kantner Terrence; McIntosh Lawrence P; Warren R Antony J; Withers Stephen G
Glycosynthase-based synthesis of xylo-oligosaccharides using an engineered retaining xylanase from Cellulomonas fimi.
Organic & biomolecular chemistry 2006;4(10):2025-32.
2006: Vaughan Mark D; Johnson Karl; DeFrees Shawn; Tang Xiaoping; Warren R Antony J; Withers Stephen G
Glycosynthase-mediated synthesis of glycosphingolipids.
Journal of the American Chemical Society 2006;128(19):6300-1.
2006: Müllegger Johannes; Chen Hong Ming; Warren R Antony J; Withers Stephen G
Glycosylation of a neoglycoprotein by using glycosynthase and thioglycoligase approaches: the generation of a thioglycoprotein.
Angewandte Chemie (International ed. in English) 2006;45(16):2585-8.
2006: Rye Carl S; Matte Allan; Cygler Miroslaw; Withers Stephen G
An atypical approach identifies TYR234 as the key base catalyst in chondroitin AC lyase.
Chembiochem : a European journal of chemical biology 2006;7(4):631-7.
2006: Yip Vivian L Y; Withers Stephen G
Breakdown of oligosaccharides by the process of elimination.
Current opinion in chemical biology 2006;10(2):147-55.
2006: Greimel Peter; Häusler Herwig; Lundt Inge; Rupitz Karen; Stütz Arnold E; Tarling Chris A; Withers Stephen G; Wrodnigg Tanja M
Fluorescent glycosidase inhibiting 1,5-dideoxy-1,5-iminoalditols.
Bioorganic & medicinal chemistry letters 2006;16(8):2067-70.
2006: Lundt Inge; Steiner Andreas J; Stütz Arnold E; Tarling Chris A; Ully Stefan; Withers Stephen G; Wrodnigg Tanja M
Fluorescently tagged iminoalditol glycosidase inhibitors as novel biological probes and diagnostics.
Bioorganic & medicinal chemistry 2006;14(6):1737-42.
2006: Kim Young-Wan; Lovering Andrew L; Chen Hongming; Kantner Terrence; McIntosh Lawrence P; Strynadka Natalie C J; Withers Stephen G
Expanding the thioglycoligase strategy to the synthesis of alpha-linked thioglycosides allows structural investigation of the parent enzyme/substrate complex.
Journal of the American Chemical Society 2006;128(7):2202-3.
2006: Watts Andrew G; Oppezzo Pablo; Withers Stephen G; Alzari Pedro M; Buschiazzo Alejandro
Structural and kinetic analysis of two covalent sialosyl-enzyme intermediates on Trypanosoma rangeli sialidase.
The Journal of biological chemistry 2006;281(7):4149-55.
2006: Yip Vivian L Y; Withers Stephen G
Mechanistic analysis of the unusual redox-elimination sequence employed by Thermotoga maritima BglT: a 6-phospho-beta-glucosidase from glycoside hydrolase family 4.
Biochemistry 2006;45(2):571-80.
2006: Williams Spencer J; Hekmat Omid; Withers Stephen G
Synthesis and testing of mechanism-based protein-profiling probes for retaining endo-glycosidases.
Chembiochem : a European journal of chemical biology 2006;7(1):116-24.
2006: Salleh Hamzah M; Müllegger Johannes; Reid Stephen P; Chan Wing Y; Hwang Jiyoung; Warren R Antony J; Withers Stephen G
Cloning and characterization of Thermotoga maritima beta-glucuronidase.
Carbohydrate research 2006;341(1):49-59.
2005: Kim Young-Wan; Chen Hongming; Withers Stephen G
Enzymatic transglycosylation of xylose using a glycosynthase.
Carbohydrate research 2005;340(18):2735-41.
2005: Duncan Gillian; Tarling Chris A; Bingle Wade H; Nomellini John F; Yamage Mat; Dorocicz Irene R; Withers Stephen G; Smit John
Evaluation of a new system for developing particulate enzymes based on the surface (S)-layer protein (RsaA) of Caulobacter crescentus: fusion with the beta-1,4-glycanase (Cex) from the cellulolytic bacterium Cellulomonas fimi yields a robust, catalytically active product.
Applied biochemistry and biotechnology 2005;127(2):95-110.
2005: Hekmat Omid; Kim Young-Wan; Williams Spencer J; He Shouming; Withers Stephen G
Active-site peptide "fingerprinting" of glycosidases in complex mixtures by mass spectrometry. Discovery of a novel retaining beta-1,4-glycanase in Cellulomonas fimi.
The Journal of biological chemistry 2005;280(42):35126-35.
2005: Vocadlo David J; Withers Stephen G
Detailed comparative analysis of the catalytic mechanisms of beta-N-acetylglucosaminidases from families 3 and 20 of glycoside hydrolases.
Biochemistry 2005;44(38):12809-18.
2005: Xia Zuyong; Azurmendi Hugo F; Lairson Luke L; Withers Stephen G; Gabelli Sandra B; Bianchet Mario A; Amzel L Mario; Mildvan Albert S
Mutational, structural, and kinetic evidence for a dissociative mechanism in the GDP-mannose mannosyl hydrolase reaction.
Biochemistry 2005;44(25):8989-97.
2005: Rempel Brian P; Clarke Lorne A; Withers Stephen G
A homology model for human alpha-l-iduronidase: insights into human disease.
Molecular genetics and metabolism 2005;85(1):28-37.
2005: Li Chunmin; Begum Anjuman; Numao Shin; Park Kwan Hwa; Withers Stephen G; Brayer Gary D
Acarbose rearrangement mechanism implied by the kinetic and structural analysis of human pancreatic alpha-amylase in complex with analogues and their elongated counterparts.
Biochemistry 2005;44(9):3347-57.
2005: Maurus Robert; Begum Anjuman; Kuo Hsin-Hen; Racaza Andrew; Numao Shin; Andersen Carsten; Tams Jeppe W; Vind Jesper; Overall Christopher M; Withers Stephen G; Brayer Gary D
Structural and mechanistic studies of chloride induced activation of human pancreatic alpha-amylase.
Protein science : a publication of the Protein Society 2005;14(3):743-55.
2005: Vocadlo David J; Withers Stephen G
The chemical synthesis of 2-deoxy-2-fluorodisaccharide probes of the hen egg white lysozyme mechanism.
Carbohydrate research 2005;340(3):379-88.
2005: Varrot Annabelle; Yip Vivian L Y; Li Yunsong; Rajan Shyamala S; Yang Xiaojing; Anderson Wayne F; Thompson John; Withers Stephen G; Davies Gideon J
NAD+ and metal-ion dependent hydrolysis by family 4 glycosidases: structural insight into specificity for phospho-beta-D-glucosides.
Journal of molecular biology 2005;346(2):423-35.
2005: Müllegger Johannes; Jahn Michael; Chen Hong-Ming; Warren R Antony J; Withers Stephen G
Engineering of a thioglycoligase: randomized mutagenesis of the acid-base residue leads to the identification of improved catalysts.
Protein engineering, design & selection : PEDS 2005;18(1):33-40.
2005: Lovering Andrew L; Lee Seung Seo; Kim Young-Wan; Withers Stephen G; Strynadka Natalie C J
Mechanistic and structural analysis of a family 31 alpha-glycosidase and its glycosyl-enzyme intermediate.
The Journal of biological chemistry 2005;280(3):2105-15.
2004: Gloster Tracey M; Macdonald James M; Tarling Chris A; Stick Robert V; Withers Stephen G; Davies Gideon J
Structural, thermodynamic, and kinetic analyses of tetrahydrooxazine-derived inhibitors bound to beta-glucosidases.
The Journal of biological chemistry 2004;279(47):49236-42.
2004: Numao Shin; Damager Iben; Li Chunmin; Wrodnigg Tanja M; Begum Anjuman; Overall Christopher M; Brayer Gary D; Withers Stephen G
In situ extension as an approach for identifying novel alpha-amylase inhibitors.
The Journal of biological chemistry 2004;279(46):48282-91.
2004: Lairson Luke L; Withers Stephen G
Mechanistic analogies amongst carbohydrate modifying enzymes.
Chemical communications (Cambridge, England) 2004;(20):2243-8.
2004: Tarling Chris A; Withers Stephen G
The synthesis of a series of modified mannotrisaccharides as probes of the enzymes involved in the early stages of mammalian complex N-glycan formation.
Carbohydrate research 2004;339(15):2487-97.
2004: Yip Vivian L Y; Withers Stephen G
Nature's many mechanisms for the degradation of oligosaccharides.
Organic & biomolecular chemistry 2004;2(19):2707-13.
2004: Kim Young-Wan; Lee Seung Seo; Warren R Antony J; Withers Stephen G
Directed evolution of a glycosynthase from Agrobacterium sp. increases its catalytic activity dramatically and expands its substrate repertoire.
The Journal of biological chemistry 2004;279(41):42787-93.
2004: Rajan Shyamala S; Yang Xiaojing; Collart Frank; Yip Vivian L Y; Withers Stephen G; Varrot Annabelle; Thompson John; Davies Gideon J; Anderson Wayne F
Novel catalytic mechanism of glycoside hydrolysis based on the structure of an NAD+/Mn2+ -dependent phospho-alpha-glucosidase from Bacillus subtilis.
Structure (London, England : 1993) 2004;12(9):1619-29.
2004: Gloster Tracey M; Williams Spencer J; Roberts Shirley; Tarling Chris A; Wicki Jacqueline; Withers Stephen G; Davies Gideon J
Atomic resolution analyses of the binding of xylobiose-derived deoxynojirimycin and isofagomine to xylanase Xyn10A.
Chemical communications (Cambridge, England) 2004;(16):1794-5.
2004: Yip Vivian L Y; Varrot Annabelle; Davies Gideon J; Rajan Shyamala S; Yang Xiaojing; Thompson John; Anderson Wayne F; Withers Stephen G
An unusual mechanism of glycoside hydrolysis involving redox and elimination steps by a family 4 beta-glycosidase from Thermotoga maritima.
Journal of the American Chemical Society 2004;126(27):8354-5.
2004: Damager Iben; Numao Shin; Chen Hongming; Brayer Gary D; Withers Stephen G
Synthesis and characterisation of novel chromogenic substrates for human pancreatic alpha-amylase.
Carbohydrate research 2004;339(10):1727-37.
2004: Wrodnigg Tanja M; Diness Frederik; Gruber Christoph; Häusler Herwig; Lundt Inge; Rupitz Karen; Steiner Andreas J; Stütz Arnold E; Tarling Chris A; Withers Stephen G; Wölfler Heidrun
Probing the aglycon binding site of a beta-glucosidase: a collection of C-1-modified 2,5-dideoxy-2,5-imino-D-mannitol derivatives and their structure-activity relationships as competitive inhibitors.
Bioorganic & medicinal chemistry 2004;12(13):3485-95.
2004: Lairson Luke L; Chiu Cecilia P C; Ly Hoa D; He Shouming; Wakarchuk Warren W; Strynadka Natalie C J; Withers Stephen G
Intermediate trapping on a mutant retaining alpha-galactosyltransferase identifies an unexpected aspartate residue.
The Journal of biological chemistry 2004;279(27):28339-44.
2004: Watts Andrew G; Withers Stephen G
Glycosynthase-catalysed formation of modified polysaccharide microstructures .
The Biochemical journal 2004;380(Pt 3):e9-10.
2004: Amaya María Fernanda; Watts Andrew G; Damager Iben; Wehenkel Annemarie; Nguyen Tong; Buschiazzo Alejandro; Paris Gastón; Frasch A Carlos; Withers Stephen G; Alzari Pedro M
Structural insights into the catalytic mechanism of Trypanosoma cruzi trans-sialidase.
Structure (London, England : 1993) 2004;12(5):775-84.
2004: Sulzenbacher Gerlind; Bignon Christophe; Nishimura Takeshi; Tarling Chris A; Withers Stephen G; Henrissat Bernard; Bourne Yves
Crystal structure of Thermotoga maritima alpha-L-fucosidase. Insights into the catalytic mechanism and the molecular basis for fucosidosis.
The Journal of biological chemistry 2004;279(13):13119-28.
2004: Rye Carl S; Withers Stephen G
The synthesis of a novel thio-linked disaccharide of chondroitin as a potential inhibitor of polysaccharide lyases.
Carbohydrate research 2004;339(3):699-703.
2004: Jahn Michael; Chen Hongming; Müllegger Johannes; Marles Jennifer; Warren R Antony J; Withers Stephen G
Thioglycosynthases: double mutant glycosidases that serve as scaffolds for thioglycoside synthesis.
Chemical communications (Cambridge, England) 2004;(3):274-5.
2004: Chiu Cecilia P C; Watts Andrew G; Lairson Luke L; Gilbert Michel; Lim Daniel; Wakarchuk Warren W; Withers Stephen G; Strynadka Natalie C J
Structural analysis of the sialyltransferase CstII from Campylobacter jejuni in complex with a substrate analog.
Nature structural & molecular biology 2004;11(2):163-70.
2004: Paal Krisztina; Ito Makoto; Withers Stephen G
Paenibacillus sp. TS12 glucosylceramidase: kinetic studies of a novel sub-family of family 3 glycosidases and identification of the catalytic residues.
The Biochemical journal 2004;378(Pt 1):141-9.
2004: Yang Jin Kuk; Yoon Hye Jin; Ahn Hyung Jun; Lee Byung Il; Pedelacq Jean-Denis; Liong Elaine C; Berendzen Joel; Laivenieks Maris; Vieille Claire; Zeikus Gregory J; Vocadlo David J; Withers Stephen G; Suh Se Won
Crystal structure of beta-D-xylosidase from Thermoanaerobacterium saccharolyticum, a family 39 glycoside hydrolase.
Journal of molecular biology 2004;335(1):155-65.
2004: Gibson Robert P; Tarling Chris A; Roberts Shirley; Withers Stephen G; Davies Gideon J
The donor subsite of trehalose-6-phosphate synthase: binary complexes with UDP-glucose and UDP-2-deoxy-2-fluoro-glucose at 2 A resolution.
The Journal of biological chemistry 2004;279(3):1950-5.
2003: Lee Seung Seo; Yu Shukun; Withers Stephen G
Detailed dissection of a new mechanism for glycoside cleavage: alpha-1,4-glucan lyase.
Biochemistry 2003;42(44):13081-90.
2003: Tarling Chris A; He Shouming; Sulzenbacher Gerlind; Bignon Christophe; Bourne Yves; Henrissat Bernard; Withers Stephen G
Identification of the catalytic nucleophile of the family 29 alpha-L-fucosidase from Thermotoga maritima through trapping of a covalent glycosyl-enzyme intermediate and mutagenesis.
The Journal of biological chemistry 2003;278(48):47394-9.
2003: Numao Shin; Kuntz Douglas A; Withers Stephen G; Rose David R
Insights into the mechanism of Drosophila melanogaster Golgi alpha-mannosidase II through the structural analysis of covalent reaction intermediates.
The Journal of biological chemistry 2003;278(48):48074-83.
2003: Hekmat Omid; Tokuyasu Ken; Withers Stephen G
Subsite structure of the endo-type chitin deacetylase from a deuteromycete, Colletotrichum lindemuthianum: an investigation using steady-state kinetic analysis and MS.
The Biochemical journal 2003;374(Pt 2):369-80.
2003: Ducros Valérie M-A; Tarling Chris A; Zechel David L; Brzozowski A Marek; Frandsen Torben P; von Ossowski Ingemar; Schülein Martin; Withers Stephen G; Davies Gideon J
Anatomy of glycosynthesis: structure and kinetics of the Humicola insolens Cel7B E197A and E197S glycosynthase mutants.
Chemistry & biology 2003;10(7):619-28.
2003: Nieman Catharine E; Wong Alexander W; He Shouming; Clarke Lorne; Hopwood John J; Withers Stephen G
Family 39 alpha-l-iduronidases and beta-D-xylosidases react through similar glycosyl-enzyme intermediates: identification of the human iduronidase nucleophile.
Biochemistry 2003;42(26):8054-65.
2003: Jahn Michael; Stoll Dominik; Warren R Antony J; Szabó Lóránd; Singh Pritpal; Gilbert Harry J; Ducros Valérie M A; Davies Gideon J; Withers Stephen G
Expansion of the glycosynthase repertoire to produce defined manno-oligosaccharides.
Chemical communications (Cambridge, England) 2003;(12):1327-9.
2003: Watts Andrew G; Damager Iben; Amaya Maria L; Buschiazzo Alejandro; Alzari Pedro; Frasch Alberto C; Withers Stephen G
Trypanosoma cruzi trans-sialidase operates through a covalent sialyl-enzyme intermediate: tyrosine is the catalytic nucleophile.
Journal of the American Chemical Society 2003;125(25):7532-3.
2003: Varrot Annabelle; Tarling Chris A; Macdonald James M; Stick Robert V; Zechel David L; Withers Stephen G; Davies Gideon J
Direct observation of the protonation state of an imino sugar glycosidase inhibitor upon binding.
Journal of the American Chemical Society 2003;125(25):7496-7.
2003: Zechel David L; Reid Stephen P; Stoll Dominik; Nashiru Oyekanmi; Warren R Antony J; Withers Stephen G
Mechanism, mutagenesis, and chemical rescue of a beta-mannosidase from cellulomonas fimi.
Biochemistry 2003;42(23):7195-204.
2003: Gloster Tracey; Williams Spencer J; Tarling Chris A; Roberts Shirley; Dupont Claude; Jodoin Pascalé; Shareck François; Withers Stephen G; Davies Gideon J
A xylobiose-derived isofagomine lactam glycosidase inhibitor binds as its amide tautomer.
Chemical communications (Cambridge, England) 2003;(8):944-5.
2003: Poon David K Y; Webster Philip; Withers Stephen G; McIntosh Lawrence P
Characterizing the pH-dependent stability and catalytic mechanism of the family 11 xylanase from the alkalophilic Bacillus agaradhaerens.
Carbohydrate research 2003;338(5):415-21.
2003: Jahn Michael; Marles Jennifer; Warren R Antony J; Withers Stephen G
Thioglycoligases: mutant glycosidases for thioglycoside synthesis.
Angewandte Chemie (International ed. in English) 2003;42(3):352-4.
2002: Withers Stephen G; Wakarchuk Warren W; Strynadka Natalie C J
One step closer to a sweet conclusion.
Chemistry & biology 2002;9(12):1270-3.
2002: Williams Spencer J; Mark Brian L; Vocadlo David J; James Michael N G; Withers Stephen G
Aspartate 313 in the Streptomyces plicatus hexosaminidase plays a critical role in substrate-assisted catalysis by orienting the 2-acetamido group and stabilizing the transition state.
The Journal of biological chemistry 2002;277(42):40055-65.
2002: Rye Carl S; Withers Stephen G
Development of an assay and determination of kinetic parameters for chondroitin AC lyase using defined synthetic substrates.
Analytical biochemistry 2002;308(1):77-82.
2002: Ducros Valérie M-A; Zechel David L; Murshudov Garib N; Gilbert Harry J; Szabó Lóránd; Stoll Dominik; Withers Stephen G; Davies Gideon J
Substrate distortion by a beta-mannanase: snapshots of the Michaelis and covalent-intermediate complexes suggest a B(2,5) conformation for the transition state.
Angewandte Chemie (International ed. in English) 2002;41(15):2824-7.
2002: Rye Carl S; Withers Stephen G
Elucidation of the mechanism of polysaccharide cleavage by chondroitin AC lyase from Flavobacterium heparinum.
Journal of the American Chemical Society 2002;124(33):9756-67.
2002: Vocadlo David J; Wicki Jacqueline; Rupitz Karen; Withers Stephen G
A case for reverse protonation: identification of Glu160 as an acid/base catalyst in Thermoanaerobacterium saccharolyticum beta-xylosidase and detailed kinetic analysis of a site-directed mutant.
Biochemistry 2002;41(31):9736-46.
2002: Vocadlo David J; Wicki Jacqueline; Rupitz Karen; Withers Stephen G
Mechanism of Thermoanaerobacterium saccharolyticum beta-xylosidase: kinetic studies.
Biochemistry 2002;41(31):9727-35.
2002: Rye Carl S; Withers Stephen G
Synthesis and evaluation of potential inhibitors of chondroitin AC lyase from Flavobacterium heparinum.
The Journal of organic chemistry 2002;67(13):4505-12.
2002: Tolborg Jakob F; Petersen Lars; Jensen Knud J; Mayer Christoph; Jakeman David L; Warren R Antony J; Withers Stephen G
Solid-phase oligosaccharide and glycopeptide synthesis using glycosynthases.
The Journal of organic chemistry 2002;67(12):4143-9.
2002: Lee Seung Seo; Yu Shukun; Withers Stephen G
alpha-1,4-Glucan lyase performs a trans-elimination via a nucleophilic displacement followed by a syn-elimination.
Journal of the American Chemical Society 2002;124(18):4948-9.
2002: Ly Hoa D; Lougheed Brenda; Wakarchuk Warren W; Withers Stephen G
Mechanistic studies of a retaining alpha-galactosyltransferase from Neisseria meningitidis.
Biochemistry 2002;41(16):5075-85.
2002: Rydberg Edwin H; Li Chunmin; Maurus Robert; Overall Christopher M; Brayer Gary D; Withers Stephen G
Mechanistic analyses of catalysis in human pancreatic alpha-amylase: detailed kinetic and structural studies of mutants of three conserved carboxylic acids.
Biochemistry 2002;41(13):4492-502.
2002: Numao Shin; Maurus Robert; Sidhu Gary; Wang Yili; Overall Christopher M; Brayer Gary D; Withers Stephen G
Probing the role of the chloride ion in the mechanism of human pancreatic alpha-amylase.
Biochemistry 2002;41(1):215-25.
2002: Wicki Jacqueline; Rose David R; Withers Stephen G
Trapping covalent intermediates on beta-glycosidases.
Methods in enzymology 2002;354():84-105.
2001: Zechel D L; Withers S G
Dissection of nucleophilic and acid-base catalysis in glycosidases.
Current opinion in chemical biology 2001;5(6):643-9.
2001: Mark B L; Vocadlo D J; Zhao D; Knapp S; Withers S G; James M N
Biochemical and structural assessment of the 1-N-azasugar GalNAc-isofagomine as a potent family 20 beta-N-acetylhexosaminidase inhibitor.
The Journal of biological chemistry 2001;276(45):42131-7.
2001: Lee S S; He S; Withers S G
Identification of the catalytic nucleophile of the Family 31 alpha-glucosidase from Aspergillus niger via trapping of a 5-fluoroglycosyl-enzyme intermediate.
The Biochemical journal 2001;359(Pt 2):381-6.
2001: Brun E; Brumer H; MacKenzie L F; Withers S G; McIntosh L P
Assignment of selectively 13C-labeled cellopentaose synthesized using an engineered glycosynthase.
Journal of biomolecular NMR 2001;21(1):67-8.
2001: Joshi M D; Sidhu G; Nielsen J E; Brayer G D; Withers S G; McIntosh L P
Dissecting the electrostatic interactions and pH-dependent activity of a family 11 glycosidase.
Biochemistry 2001;40(34):10115-39.
2001: Blanchard J E; Withers S G
Rapid screening of the aglycone specificity of glycosidases: applications to enzymatic synthesis of oligosaccharides.
Chemistry & biology 2001;8(7):627-33.
2001: Blanchard J E; Gal L; He S; Foisy J; Warren R A; Withers S G
The identification of the catalytic nucleophiles of two beta-galactosidases from glycoside hydrolase family 35.
Carbohydrate research 2001;333(1):7-17.
2001: Zechel D L; Reid S P; Nashiru O; Mayer C; Stoll D; Jakeman D L; Warren R A; Withers S G
Enzymatic synthesis of carbon-fluorine bonds.
Journal of the American Chemical Society 2001;123(18):4350-1.
2001: Hermetter A; Scholze H; Stütz A E; Withers S G; Wrodnigg T M
Powerful probes for glycosidases: novel, fluorescently tagged glycosidase inhibitors.
Bioorganic & medicinal chemistry letters 2001;11(10):1339-42.
2001: Mayer C; Jakeman D L; Mah M; Karjala G; Gal L; Warren R A; Withers S G
Directed evolution of new glycosynthases from Agrobacterium beta-glucosidase: a general screen to detect enzymes for oligosaccharide synthesis.
Chemistry & biology 2001;8(5):437-43.
2001: Wrodnigg T M; Withers S G; Stütz A E
Novel, lipophilic derivatives of 2,5-dideoxy-2,5-imino-D-mannitol (DMDP) are powerful beta-glucosidase inhibitors.
Bioorganic & medicinal chemistry letters 2001;11(8):1063-4.
2001: Mark B L; Vocadlo D J; Knapp S; Triggs-Raine B L; Withers S G; James M N
Crystallographic evidence for substrate-assisted catalysis in a bacterial beta-hexosaminidase.
The Journal of biological chemistry 2001;276(13):10330-7.
2001: Hou Y; Vocadlo D J; Leung A; Withers S G; Mahuran D
Characterization of the Glu and Asp residues in the active site of human beta-hexosaminidase B.
Biochemistry 2001;40(7):2201-9.
2001: Persson K; Ly H D; Dieckelmann M; Wakarchuk W W; Withers S G; Strynadka N C
Crystal structure of the retaining galactosyltransferase LgtC from Neisseria meningitidis in complex with donor and acceptor sugar analogs.
Nature structural biology 2001;8(2):166-75.
2000: Ly H D; Howard S; Shum K; He S; Zhu A; Withers S G
The synthesis, testing and use of 5-fluoro-alpha-D-galactosyl fluoride to trap an intermediate on green coffee bean alpha-galactosidase and identify the catalytic nucleophile.
Carbohydrate research 2000;329(3):539-47.
2000: Numao S; He S; Evjen G; Howard S; Tollersrud O K; Withers S G
Identification of Asp197 as the catalytic nucleophile in the family 38 alpha-mannosidase from bovine kidney lysosomes.
FEBS letters 2000;484(3):175-8.
2000: Stoll D; He S; Withers S G; Warren R A
Identification of Glu-519 as the catalytic nucleophile in beta-mannosidase 2A from Cellulomonas fimi.
The Biochemical journal 2000;351 Pt 3():833-8.
2000: Rye C S; Withers S G
Glycosidase mechanisms.
Current opinion in chemical biology 2000;4(5):573-80.
2000: Notenboom V; Williams S J; Hoos R; Withers S G; Rose D R
Detailed structural analysis of glycosidase/inhibitor interactions: complexes of Cex from Cellulomonas fimi with xylobiose-derived aza-sugars.
Biochemistry 2000;39(38):11553-63.
2000: Hart D O; He S; Chany C J; Withers S G; Sims P F; Sinnott M L; Brumer H
Identification of Asp-130 as the catalytic nucleophile in the main alpha-galactosidase from Phanerochaete chrysosporium, a family 27 glycosyl hydrolase.
Biochemistry 2000;39(32):9826-36.
2000: Williams S J; Withers S G
Glycosyl fluorides in enzymatic reactions.
Carbohydrate research 2000;327(1-2):27-46.
2000: Joshi M D; Sidhu G; Pot I; Brayer G D; Withers S G; McIntosh L P
Hydrogen bonding and catalysis: a novel explanation for how a single amino acid substitution can change the pH optimum of a glycosidase.
Journal of molecular biology 2000;299(1):255-79.
2000: Brayer G D; Sidhu G; Maurus R; Rydberg E H; Braun C; Wang Y; Nguyen N T; Overall C M; Withers S G
Subsite mapping of the human pancreatic alpha-amylase active site through structural, kinetic, and mutagenesis techniques.
Biochemistry 2000;39(16):4778-91.
2000: Connelly G P; Withers S G; McIntosh L P
Analysis of the dynamic properties of Bacillus circulans xylanase upon formation of a covalent glycosyl-enzyme intermediate.
Protein science : a publication of the Protein Society 2000;9(3):512-24.
2000: Mayer C; Zechel D L; Reid S P; Warren R A; Withers S G
The E358S mutant of Agrobacterium sp. beta-glucosidase is a greatly improved glycosynthase.
FEBS letters 2000;466(1):40-4.
2000: Zechel D L; Withers S G
Glycosidase mechanisms: anatomy of a finely tuned catalyst.
Accounts of chemical research 2000;33(1):11-8.
2000: Vocadlo D J; Mayer C; He S; Withers S G
Mechanism of action and identification of Asp242 as the catalytic nucleophile of Vibrio furnisii N-acetyl-beta-D-glucosaminidase using 2-acetamido-2-deoxy-5-fluoro-alpha-L-idopyranosyl fluoride.
Biochemistry 2000;39(1):117-26.
2000: Vocadlo D J; Withers S G
Identification of active site residues in glycosidases by use of tandem mass spectrometry.
Methods in molecular biology (Clifton, N.J.) 2000;146():203-22.
1999: Lougheed B; Ly H D; Wakarchuk W W; Withers S G
Glycosyl fluorides can function as substrates for nucleotide phosphosugar-dependent glycosyltransferases.
The Journal of biological chemistry 1999;274(53):37717-22.
1999: Uitdehaag J C; Mosi R; Kalk K H; van der Veen B A; Dijkhuizen L; Withers S G; Dijkstra B W
X-ray structures along the reaction pathway of cyclodextrin glycosyltransferase elucidate catalysis in the alpha-amylase family.
Nature structural biology 1999;6(5):432-6.
1999: Sidhu G; Withers S G; Nguyen N T; McIntosh L P; Ziser L; Brayer G D
Sugar ring distortion in the glycosyl-enzyme intermediate of a family G/11 xylanase.
Biochemistry 1999;38(17):5346-54.
1999: Sulzenbacher G; Mackenzie L F; Wilson K S; Withers S G; Dupont C; Davies G J
The crystal structure of a 2-fluorocellotriosyl complex of the Streptomyces lividans endoglucanase CelB2 at 1.2 A resolution.
Biochemistry 1999;38(15):4826-33.
1999: Johnson P E; Brun E; MacKenzie L F; Withers S G; McIntosh L P
The cellulose-binding domains from Cellulomonas fimi beta-1, 4-glucanase CenC bind nitroxide spin-labeled cellooligosaccharides in multiple orientations.
Journal of molecular biology 1999;287(3):609-25.
1999: Rydberg E H; Sidhu G; Vo H C; Hewitt J; Côte H C; Wang Y; Numao S; MacGillivray R T; Overall C M; Brayer G D; Withers S G
Cloning, mutagenesis, and structural analysis of human pancreatic alpha-amylase expressed in Pichia pastoris.
Protein science : a publication of the Protein Society 1999;8(3):635-43.
1999: Mosi R; Withers S G
Synthesis and kinetic evaluation of 4-deoxymaltopentaose and 4-deoxymaltohexaose as inhibitors of muscle and potato alpha-glucan phosphorylases.
The Biochemical journal 1999;338 ( Pt 2)():251-6.
1998: Mosi R; Sham H; Uitdehaag J C; Ruiterkamp R; Dijkstra B W; Withers S G
Reassessment of acarbose as a transition state analogue inhibitor of cyclodextrin glycosyltransferase.
Biochemistry 1998;37(49):17192-8.
1998: Wong A W; He S; Grubb J H; Sly W S; Withers S G
Identification of Glu-540 as the catalytic nucleophile of human beta-glucuronidase using electrospray mass spectrometry.
The Journal of biological chemistry 1998;273(51):34057-62.
1998: Zechel D L; He S; Dupont C; Withers S G
Identification of Glu-120 as the catalytic nucleophile in Streptomyces lividans endoglucanase celB.
The Biochemical journal 1998;336 ( Pt 1)():139-45.
1998: Vocadlo D J; MacKenzie L F; He S; Zeikus G J; Withers S G
Identification of glu-277 as the catalytic nucleophile of Thermoanaerobacterium saccharolyticum beta-xylosidase using electrospray MS.
The Biochemical journal 1998;335 ( Pt 2)():449-55.
1998: MacKenzie L F; Sulzenbacher G; Divne C; Jones T A; Wöldike H F; Schülein M; Withers S G; Davies G J
Crystal structure of the family 7 endoglucanase I (Cel7B) from Humicola insolens at 2.2 A resolution and identification of the catalytic nucleophile by trapping of the covalent glycosyl-enzyme intermediate.
The Biochemical journal 1998;335 ( Pt 2)():409-16.
1998: Notenboom V; Birsan C; Nitz M; Rose D R; Warren R A; Withers S G
Insights into transition state stabilization of the beta-1,4-glycosidase Cex by covalent intermediate accumulation in active site mutants.
Nature structural biology 1998;5(9):812-8.
1998: Davies G J; Mackenzie L; Varrot A; Dauter M; Brzozowski A M; Schülein M; Withers S G
Snapshots along an enzymatic reaction coordinate: analysis of a retaining beta-glycoside hydrolase.
Biochemistry 1998;37(34):11707-13.
1998: Birsan C; Johnson P; Joshi M; MacLeod A; McIntosh L; Monem V; Nitz M; Rose D R; Tull D; Wakarchuck W W; Wang Q; Warren R A; White A; Withers S G
Mechanisms of cellulases and xylanases.
Biochemical Society transactions 1998;26(2):156-60.
1998: Zechel D L; Konermann L; Withers S G; Douglas D J
Pre-steady state kinetic analysis of an enzymatic reaction monitored by time-resolved electrospray ionization mass spectrometry.
Biochemistry 1998;37(21):7664-9.
1998: Notenboom V; Birsan C; Warren R A; Withers S G; Rose D R
Exploring the cellulose/xylan specificity of the beta-1,4-glycanase cex from Cellulomonas fimi through crystallography and mutation.
Biochemistry 1998;37(14):4751-8.
1998: Howard S; Withers S G
Labeling and identification of the postulated acid/base catalyst in the alpha-glucosidase from Saccharomyces cerevisiae using a novel bromoketone C-glycoside.
Biochemistry 1998;37(11):3858-64.
1998: Lawson S L; Warren R A; Withers S G
Mechanistic consequences of replacing the active-site nucleophile Glu-358 in Agrobacterium sp. beta-glucosidase with a cysteine residue.
The Biochemical journal 1998;330 ( Pt 1)():203-9.
1998: Howard S; He S; Withers S G
Identification of the active site nucleophile in jack bean alpha-mannosidase using 5-fluoro-beta-L-gulosyl fluoride.
The Journal of biological chemistry 1998;273(4):2067-72.
1997: He S; Withers S G
Assignment of sweet almond beta-glucosidase as a family 1 glycosidase and identification of its active site nucleophile.
The Journal of biological chemistry 1997;272(40):24864-7.
1997: Howard S; Braun C; McCarter J; Moremen K W; Liao Y F; Withers S G
Human lysosomal and jack bean alpha-mannosidases are retaining glycosidases.
Biochemical and biophysical research communications 1997;238(3):896-8.
1997: Mosi R; He S; Uitdehaag J; Dijkstra B W; Withers S G
Trapping and characterization of the reaction intermediate in cyclodextrin glycosyltransferase by use of activated substrates and a mutant enzyme.
Biochemistry 1997;36(32):9927-34.
1997: Mackenzie L F; Davies G J; Schülein M; Withers S G
Identification of the catalytic nucleophile of endoglucanase I from Fusarium oxysporum by mass spectrometry.
Biochemistry 1997;36(19):5893-901.
1997: Lawson S L; Wakarchuk W W; Withers S G
Positioning the acid/base catalyst in a glycosidase: studies with Bacillus circulans xylanase.
Biochemistry 1997;36(8):2257-65.
1997: Mackenzie L F; Brooke G S; Cutfield J F; Sullivan P A; Withers S G
Identification of Glu-330 as the catalytic nucleophile of Candida albicans exo-beta-(1,3)-glucanase.
The Journal of biological chemistry 1997;272(6):3161-7.
1997: McCarter J D; Burgoyne D L; Miao S; Zhang S; Callahan J W; Withers S G
Identification of Glu-268 as the catalytic nucleophile of human lysosomal beta-galactosidase precursor by mass spectrometry.
The Journal of biological chemistry 1997;272(1):396-400.
1996: Lai E C; Morris S A; Street I P; Withers S G
Substituted glycals as probes of glycosidase mechanisms.
Bioorganic & medicinal chemistry 1996;4(11):1929-37.
1996: Stirtan W G; Withers S G
Phosphonate and alpha-fluorophosphonate analogue probes of the ionization state of pyridoxal 5'-phosphate (PLP) in glycogen phosphorylase.
Biochemistry 1996;35(47):15057-64.
1996: MacLeod A M; Tull D; Rupitz K; Warren R A; Withers S G
Mechanistic consequences of mutation of active site carboxylates in a retaining beta-1,4-glycanase from Cellulomonas fimi.
Biochemistry 1996;35(40):13165-72.
1996: Lawson S L; Wakarchuk W W; Withers S G
Effects of both shortening and lengthening the active site nucleophile of Bacillus circulans xylanase on catalytic activity.
Biochemistry 1996;35(31):10110-8.
1996: McIntosh L P; Hand G; Johnson P E; Joshi M D; Körner M; Plesniak L A; Ziser L; Wakarchuk W W; Withers S G
The pKa of the general acid/base carboxyl group of a glycosidase cycles during catalysis: a 13C-NMR study of bacillus circulans xylanase.
Biochemistry 1996;35(31):9958-66.
1996: Damude H G; Ferro V; Withers S G; Warren R A
Substrate specificity of endoglucanase A from Cellulomonas fimi: fundamental differences between endoglucanases and exoglucanases from family 6.
The Biochemical journal 1996;315 ( Pt 2)():467-72.
1996: Braun C; Lindhorst T; Madsen N B; Withers S G
Identification of Asp 549 as the catalytic nucleophile of glycogen-debranching enzyme via trapping of the glycosyl-enzyme intermediate.
Biochemistry 1996;35(17):5458-63.
1996: McCarter J D; Withers S G
Unequivocal identification of Asp-214 as the catalytic nucleophile of Saccharomyces cerevisiae alpha-glucosidase using 5-fluoro glycosyl fluorides.
The Journal of biological chemistry 1996;271(12):6889-94.
1996: Tull D; Burgoyne D L; Chow D T; Withers S G; Aebersold R
A mass spectrometry-based approach for probing enzyme active sites: identification of Glu 127 in Cellulomonas fimi exoglycanase as the residue modified by N-bromoacetyl cellobiosylamine.
Analytical biochemistry 1996;234(2):119-25.
1996: White A; Tull D; Johns K; Withers S G; Rose D R
Crystallographic observation of a covalent catalytic intermediate in a beta-glycosidase.
Nature structural biology 1996;3(2):149-54.
1995: Namchuk M N; Withers S G
Mechanism of Agrobacterium beta-glucosidase: kinetic analysis of the role of noncovalent enzyme/substrate interactions.
Biochemistry 1995;34(49):16194-202.
1995: Braun C; Brayer G D; Withers S G
Mechanism-based inhibition of yeast alpha-glucosidase and human pancreatic alpha-amylase by a new class of inhibitors. 2-Deoxy-2,2-difluoro-alpha-glycosides.
The Journal of biological chemistry 1995;270(45):26778-81.
1995: Wang Q; Trimbur D; Graham R; Warren R A; Withers S G
Identification of the acid/base catalyst in Agrobacterium faecalis beta-glucosidase by kinetic analysis of mutants.
Biochemistry 1995;34(44):14554-62.
1995: Gebler J C; Trimbur D E; Warren A J; Aebersold R; Namchuk M; Withers S G
Substrate-induced inactivation of a crippled beta-glucosidase mutant: identification of the labeled amino acid and mutagenic analysis of its role.
Biochemistry 1995;34(44):14547-53.
1995: Brayer G D; Luo Y; Withers S G
The structure of human pancreatic alpha-amylase at 1.8 A resolution and comparisons with related enzymes.
Protein science : a publication of the Protein Society 1995;4(9):1730-42.
1995: Ziser L; Setyawati I; Withers S G
Syntheses and testing of substrates and mechanism-based inactivators for xylanases.
Carbohydrate research 1995;274():137-53.
1995: Braun C; Withers S G
An assay for the transferase activity of glycogen debranching enzyme.
Carbohydrate research 1995;271(1):113-8.
1995: Street I P; Withers S G
Probing the ionization state of substrate alpha-D-glucopyranosyl phosphate bound to glycogen phosphorylase b.
The Biochemical journal 1995;308 ( Pt 3)():1017-23.
1995: Withers S G; Aebersold R
Approaches to labeling and identification of active site residues in glycosidases.
Protein science : a publication of the Protein Society 1995;4(3):361-72.
1995: Damude H G; Withers S G; Kilburn D G; Miller R C; Warren R A
Site-directed mutation of the putative catalytic residues of endoglucanase CenA from Cellulomonas fimi.
Biochemistry 1995;34(7):2220-4.
1995: Tull D; Miao S; Withers S G; Aebersold R
Identification of derivatized peptides without radiolabels: tandem mass spectrometric localization of the tagged active-site nucleophiles of two cellulases and a beta-glucosidase.
Analytical biochemistry 1995;224(2):509-14.
1994: Lai E C; Withers S G
Stereochemistry and kinetics of the hydration of 2-acetamido-D-glucal by beta-N-acetylhexosaminidases.
Biochemistry 1994;33(49):14743-9.
1994: McCarter J D; Withers S G
Mechanisms of enzymatic glycoside hydrolysis.
Current opinion in structural biology 1994;4(6):885-92.
1994: Zhang S; McCarter J D; Okamura-Oho Y; Yaghi F; Hinek A; Withers S G; Callahan J W
Kinetic mechanism and characterization of human beta-galactosidase precursor secreted by permanently transfected Chinese hamster ovary cells.
The Biochemical journal 1994;304 ( Pt 1)():281-8.
1994: White A; Withers S G; Gilkes N R; Rose D R
Crystal structure of the catalytic domain of the beta-1,4-glycanase cex from Cellulomonas fimi.
Biochemistry 1994;33(42):12546-52.
1994: McCarter J D; Adam M J; Hartman N G; Withers S G
In vivo inhibition of beta-glucosidase and beta-mannosidase activity in rats by 2-deoxy-2-fluoro-beta-glycosyl fluorides and recovery of activity in vivo and in vitro.
The Biochemical journal 1994;301 ( Pt 2)():343-8.
1994: Miao S; Ziser L; Aebersold R; Withers S G
Identification of glutamic acid 78 as the active site nucleophile in Bacillus subtilis xylanase using electrospray tandem mass spectrometry.
Biochemistry 1994;33(23):7027-32.
1994: Tull D; Withers S G
Mechanisms of cellulases and xylanases: a detailed kinetic study of the exo-beta-1,4-glycanase from Cellulomonas fimi.
Biochemistry 1994;33(20):6363-70.
1994: MacLeod A M; Lindhorst T; Withers S G; Warren R A
The acid/base catalyst in the exoglucanase/xylanase from Cellulomonas fimi is glutamic acid 127: evidence from detailed kinetic studies of mutants.
Biochemistry 1994;33(20):6371-6.
1994: Wang Q; Dechert U; Jirik F; Withers S G
Suicide inactivation of human prostatic acid phosphatase and a phosphotyrosine phosphatase.
Biochemical and biophysical research communications 1994;200(1):577-83.
1994: Miao S; McCarter J D; Grace M E; Grabowski G A; Aebersold R; Withers S G
Identification of Glu340 as the active-site nucleophile in human glucocerebrosidase by use of electrospray tandem mass spectrometry.
The Journal of biological chemistry 1994;269(15):10975-8.
1993: Black T S; Kiss L; Tull D; Withers S G
N-bromoacetyl-glycopyranosylamines as affinity labels for a beta-glucosidase and a cellulase.
Carbohydrate research 1993;250(1):195-202.
1993: McCarter J D; Adam M J; Braun C; Namchuk M; Tull D; Withers S G
Syntheses of 2-deoxy-2-fluoro mono- and oligo-saccharide glycosides from glycals and evaluation as glycosidase inhibitors.
Carbohydrate research 1993;249(1):77-90.
1993: Braun C; Meinke A; Ziser L; Withers S G
Simultaneous high-performance liquid chromatographic determination of both the cleavage pattern and the stereochemical outcome of the hydrolysis reactions catalyzed by various glycosidases.
Analytical biochemistry 1993;212(1):259-62.
1993: Wang Q; Tull D; Meinke A; Gilkes N R; Warren R A; Aebersold R; Withers S G
Glu280 is the nucleophile in the active site of Clostridium thermocellum CelC, a family A endo-beta-1,4-glucanase.
The Journal of biological chemistry 1993;268(19):14096-102.
1993: Burk D; Wang Y; Dombroski D; Berghuis A M; Evans S V; Luo Y; Withers S G; Brayer G D
Isolation, crystallization and preliminary diffraction analyses of human pancreatic alpha-amylase.
Journal of molecular biology 1993;230(3):1084-5.
1993: Challoner R; McDowell C A; Stirtan W; Withers S G
A solid-state 31P-NMR investigation of the allosteric transition in glycogen phosphorylase b.
Biophysical journal 1993;64(2):484-91.
1992: Street I P; Kempton J B; Withers S G
Inactivation of a beta-glucosidase through the accumulation of a stable 2-deoxy-2-fluoro-alpha-D-glucopyranosyl-enzyme intermediate: a detailed investigation.
Biochemistry 1992;31(41):9970-8.
1992: Kempton J B; Withers S G
Mechanism of Agrobacterium beta-glucosidase: kinetic studies.
Biochemistry 1992;31(41):9961-9.
1992: Withers S G; Rupitz K; Trimbur D; Warren R A
Mechanistic consequences of mutation of the active site nucleophile Glu 358 in Agrobacterium beta-glucosidase.
Biochemistry 1992;31(41):9979-85.
1992: McCarter J D; Adam M J; Withers S G
Binding energy and catalysis. Fluorinated and deoxygenated glycosides as mechanistic probes of Escherichia coli (lacZ) beta-galactosidase.
The Biochemical journal 1992;286 ( Pt 3)():721-7.
1992: Gebler J; Gilkes N R; Claeyssens M; Wilson D B; Béguin P; Wakarchuk W W; Kilburn D G; Miller R C; Warren R A; Withers S G
Stereoselective hydrolysis catalyzed by related beta-1,4-glucanases and beta-1,4-xylanases.
The Journal of biological chemistry 1992;267(18):12559-61.
1992: Gebler J C; Aebersold R; Withers S G
Glu-537, not Glu-461, is the nucleophile in the active site of (lac Z) beta-galactosidase from Escherichia coli.
The Journal of biological chemistry 1992;267(16):11126-30.
1992: Trimbur D E; Warren R A; Withers S G
Region-directed mutagenesis of residues surrounding the active site nucleophile in beta-glucosidase from Agrobacterium faecalis.
The Journal of biological chemistry 1992;267(15):10248-51.
1992: Percival M D; Withers S G
19F NMR investigations of the catalytic mechanism of phosphoglucomutase using fluorinated substrates and inhibitors.
Biochemistry 1992;31(2):505-12.
1992: Percival M D; Withers S G
Binding energy and catalysis: deoxyfluoro sugars as probes of hydrogen bonding in phosphoglucomutase.
Biochemistry 1992;31(2):498-505.
1991: Tull D; Withers S G; Gilkes N R; Kilburn D G; Warren R A; Aebersold R
Glutamic acid 274 is the nucleophile in the active site of a "retaining" exoglucanase from Cellulomonas fimi.
The Journal of biological chemistry 1991;266(24):15621-5.
1991: Withers S G; Umezawa K
Cyclophellitol: a naturally occurring mechanism-based inactivator of beta-glucosidases.
Biochemical and biophysical research communications 1991;177(1):532-7.
1990: Withers S G; Rupitz K
Measurement of active-site homology between potato and rabbit muscle alpha-glucan phosphorylases through use of a linear free energy relationship.
Biochemistry 1990;29(27):6405-9.
1989: Caron G; Withers S G
Conduritol aziridine: a new mechanism-based glucosidase inactivator.
Biochemical and biophysical research communications 1989;163(1):495-9.
1989: Street I P; Rupitz K; Withers S G
Fluorinated and deoxygenated substrates as probes of transition-state structure in glycogen phosphorylase.
Biochemistry 1989;28(4):1581-7.
1988: Withers S G; Rupitz K; Street I P
2-Deoxy-2-fluoro-D-glycosyl fluorides. A new class of specific mechanism-based glycosidase inhibitors.
The Journal of biological chemistry 1988;263(17):7929-32.
1988: Holme K R; Hall L D; Armstrong C R; Withers S G
Synthesis and evaluation of chitosan-based, affinity-chromatography materials.
Carbohydrate research 1988;173(2):285-91.

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