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Alfred Wittinghofer

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Denise Wätzlich; Alfred Wittinghofer; Yong-Xiang Chen; Katja Gotthardt; Mandy Miertzschke; Ingrid Vetter; Shehab Ismail
The interplay between RPGR, PDEδ and Arl2/3 regulate the ciliary targeting of farnesylated cargo.
Begoña Sot; Elmar Behrmann; Stefan Raunser; Alfred Wittinghofer
Ras GTPase activating (RasGAP) activity of the dual specificity GAP protein Rasal requires colocalization and C2 domain binding to lipid membranes.
Shehab A Ismail; Carolin Koerner; Mandy Miertzschke; Ingrid R Vetter; Yong-Xiang Chen; Alfred Wittinghofer
Structural basis for Arl3-specific release of myristoylated ciliary cargo from UNC119.

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2013: Denise Wätzlich; Alfred Wittinghofer; Yong-Xiang Chen; Katja Gotthardt; Mandy Miertzschke; Ingrid Vetter; Shehab Ismail
The interplay between RPGR, PDEδ and Arl2/3 regulate the ciliary targeting of farnesylated cargo.
EMBO reports 2013;14(5):465-72.
2013: Begoña Sot; Elmar Behrmann; Stefan Raunser; Alfred Wittinghofer
Ras GTPase activating (RasGAP) activity of the dual specificity GAP protein Rasal requires colocalization and C2 domain binding to lipid membranes.
Proceedings of the National Academy of Sciences of the United States of America 2013;110(1):111-6.
2012: Shehab A Ismail; Carolin Koerner; Mandy Miertzschke; Ingrid R Vetter; Yong-Xiang Chen; Alfred Wittinghofer
Structural basis for Arl3-specific release of myristoylated ciliary cargo from UNC119.
The EMBO journal 2012;31(20):4085-94.
2012: Bernd K Gilsbach; Franz Y Ho; Peter J M van Haastert; Ingrid R Vetter; Alfred Wittinghofer; Arjan Kortholt
Roco kinase structures give insights into the mechanism of Parkinson disease-related leucine-rich-repeat kinase 2 mutations.
Proceedings of the National Academy of Sciences of the United States of America 2012;109(26):10322-7.
2012: Anchal Chandra; Hernán E Grecco; Michael Hanzal-Bayer; Christian Hedberg; Shehab A Ismail; David Perera; Venkat Pisupati; Ferdinandos Skoulidis; Ashok R Venkitaraman; Alfred Wittinghofer; Liam Cassidy; Philippe I H Bastiaens
The GDI-like solubilizing factor PDEδ sustains the spatial organization and signalling of Ras family proteins.
Nature cell biology 2012;14(2):148-58.
2011: Shehab A Ismail; Alexandra Rusinova; Gemma Triola; Herbert Waldmann; Philippe I H Bastiaens; Martin Bierbaum; Anchal Chandra; Yong-Xiang Chen; Lothar Gremer; Alfred Wittinghofer
Arl2-GTP and Arl3-GTP regulate a GDI-like transport system for farnesylated cargo.
Nature chemical biology 2011;7(12):942-9.
2011: Elena Mucha; Antje Schaefer; Alfred Wittinghofer; Inka Fricke; Antje Berken
Rho proteins of plants--functional cycle and regulation of cytoskeletal dynamics.
European journal of cell biology 2011;90(11):934-43.
2011: Mandy Miertzschke; Lotte Søgaard-Andersen; Ingrid R Vetter; Edina Hot; Daniela Keilberg; Carolin Koerner; Simone Leonardy; Alfred Wittinghofer
Structural analysis of the Ras-like G protein MglA and its cognate GAP MglB and implications for bacterial polarity.
The EMBO journal 2011;30(20):4185-97.
2011: Antje Schaefer; Antje Berken; Mandy Miertzschke; Alfred Wittinghofer
Dimeric plant RhoGAPs are regulated by its CRIB effector motif to stimulate a sequential GTP hydrolysis.
Journal of molecular biology 2011;411(4):808-22.
2011: Eldar Zent; Ingrid Vetter; Alfred Wittinghofer
Structural and biochemical properties of Sept7, a unique septin required for filament formation.
Biological chemistry 2011;392(8-9):791-7.
2011: Alfred Wittinghofer
Highlight on septins.
Biological chemistry 2011;392(8-9):679-80.
2011: Alfred Wittinghofer; Ingrid R Vetter
Structure-function relationships of the G domain, a canonical switch motif.
Annual review of biochemistry 2011;80():943-71.
2011: Antje Schaefer; Antje Berken; Kathrin Höhner; Alfred Wittinghofer
The unique plant RhoGAPs are dimeric and contain a CRIB motif required for affinity and specificity towards cognate small G proteins.
Biopolymers 2011;95(6):420-33.
2011: Lothar Gremer; Torsten Merbitz-Zahradnik; Radovan Dvorsky; Ion C Cirstea; Christian Peter Kratz; Martin Zenker; Alfred Wittinghofer; Mohammad Reza Ahmadian
Germline KRAS mutations cause aberrant biochemical and physical properties leading to developmental disorders.
Human mutation 2011;32(1):33-43.
2010: Gia Machaidze; Andrea Sokoll; Atsushi Shimada; Ariel Lustig; Antonina Mazur; Alfred Wittinghofer; Ueli Aebi; Hans Georg Mannherz
Actin filament bundling and different nucleating effects of mouse Diaphanous-related formin FH2 domains on actin/ADF and actin/cofilin complexes.
Journal of molecular biology 2010;403(4):529-45.
2010: Yong-Xiang Chen; Sebastian Koch; Katharina Uhlenbrock; Katrin Weise; Debapratim Das; Lothar Gremer; Luc Brunsveld; Alfred Wittinghofer; Roland Winter; Gemma Triola; Herbert Waldmann
Synthesis of the Rheb and K-Ras4B GTPases.
Angewandte Chemie (International ed. in English) 2010;49(35):6090-5.
2010: Simone Leonardy; Mandy Miertzschke; Iryna Bulyha; Eva Sperling; Alfred Wittinghofer; Lotte Søgaard-Andersen
Regulation of dynamic polarity switching in bacteria by a Ras-like G-protein and its cognate GAP.
The EMBO journal 2010;29(14):2276-89.
2010: Shehab A Ismail; Ingrid R Vetter; Begona Sot; Alfred Wittinghofer
The structure of an Arf-ArfGAP complex reveals a Ca2+ regulatory mechanism.
Cell 2010;141(5):812-21.
2010: Sabine Böhme; Simon Meyer; André Krüger; Heinz-Jürgen Steinhoff; Alfred Wittinghofer; Johann P Klare
Stabilization of G domain conformations in the tRNA-modifying MnmE-GidA complex observed with double electron electron resonance spectroscopy.
The Journal of biological chemistry 2010;285(22):16991-7000.
2010: Ina C Rosnizeck; Thorsten Graf; Michael Spoerner; Jens Tränkle; Daniel Filchtinski; Christian Herrmann; Lothar Gremer; Ingrid R Vetter; Alfred Wittinghofer; Burkhard König; Hans Robert Kalbitzer
Stabilizing a weak binding state for effectors in the human ras protein by cyclen complexes.
Angewandte Chemie (International ed. in English) 2010;49(22):3830-3.
2010: Begoña Sot; Carsten Kötting; Delia Deaconescu; Yan Suveyzdis; Klaus Gerwert; Alfred Wittinghofer
Unravelling the mechanism of dual-specificity GAPs.
The EMBO journal 2010;29(7):1205-14.
2010: Arjan Kortholt; Parvin Bolourani; Holger Rehmann; Ineke Keizer-Gunnink; Gerald Weeks; Alfred Wittinghofer; Peter J M Van Haastert
A Rap/phosphatidylinositol 3-kinase pathway controls pseudopod formation [corrected].
Molecular biology of the cell 2010;21(6):936-45.
2009: Simon Meyer; Alfred Wittinghofer; Wim Versées
G-domain dimerization orchestrates the tRNA wobble modification reaction in the MnmE/GidA complex.
Journal of molecular biology 2009;392(4):910-22.
2009: Simon Meyer; Sabine Böhme; André Krüger; Heinz-Jürgen Steinhoff; Johann P Klare; Alfred Wittinghofer
Kissing G domains of MnmE monitored by X-ray crystallography and pulse electron paramagnetic resonance spectroscopy.
PLoS biology 2009;7(10):e1000212.
2009: Stefan Veltel; Alfred Wittinghofer
RPGR and RP2: targets for the treatment of X-linked retinitis pigmentosa?
Expert opinion on therapeutic targets 2009;13(10):1239-51.
2009: Minhajuddin Sirajuddin; Marian Farkasovsky; Eldar Zent; Alfred Wittinghofer
GTP-induced conformational changes in septins and implications for function.
Proceedings of the National Academy of Sciences of the United States of America 2009;106(39):16592-7.
2009: Elif Yaman; Raphael Gasper; Carolin Koerner; Alfred Wittinghofer; Uygar H Tazebay
RasGEF1A and RasGEF1B are guanine nucleotide exchange factors that discriminate between Rap GTP-binding proteins and mediate Rap2-specific nucleotide exchange.
The FEBS journal 2009;276(16):4607-16.
2009: Raphael Gasper; Simon Meyer; Katja Gotthardt; Minhajuddin Sirajuddin; Alfred Wittinghofer
It takes two to tango: regulation of G proteins by dimerization.
Nature reviews. Molecular cell biology 2009;10(6):423-9.
2009: Katharina Uhlenbrock; Matthias Weiwad; Reinhard Wetzker; Gunter Fischer; Alfred Wittinghofer; Ignacio Rubio
Reassessment of the role of FKBP38 in the Rheb/mTORC1 pathway.
FEBS letters 2009;583(6):965-70.
2009: Christian Ottmann; Michael Weyand; Takeshi Sassa; Takatsugu Inoue; Nobuo Kato; Alfred Wittinghofer; Claudia Oecking
A structural rationale for selective stabilization of anti-tumor interactions of 14-3-3 proteins by cotylenin A.
Journal of molecular biology 2009;386(4):913-9.
2008: Michael Lammers; Simon Meyer; Dorothee Kühlmann; Alfred Wittinghofer
Specificity of interactions between mDia isoforms and Rho proteins.
The Journal of biological chemistry 2008;283(50):35236-46.
2008: Holger Rehmann; M Brüning; C Berghaus; M Schwarten; K Köhler; H Stocker; R Stoll; Fried J T Zwartkruis; Alfred Wittinghofer
Biochemical characterisation of TCTP questions its function as a guanine nucleotide exchange factor for Rheb.
FEBS letters 2008;582(20):3005-10.
2008: Lothar Gremer; Bernd Gilsbach; Mohammad Reza Ahmadian; Alfred Wittinghofer
Fluoride complexes of oncogenic Ras mutants to study the Ras-RasGap interaction.
Biological chemistry 2008;389(9):1163-71.
2008: Katja Gotthardt; Michael Weyand; Arjan Kortholt; Peter J M Van Haastert; Alfred Wittinghofer
Structure of the Roc-COR domain tandem of C. tepidum, a prokaryotic homologue of the human LRRK2 Parkinson kinase.
The EMBO journal 2008;27(16):2239-49.
2008: Stefan Veltel; Aleksandra Kravchenko; Shehab Ismail; Alfred Wittinghofer
Specificity of Arl2/Arl3 signaling is mediated by a ternary Arl3-effector-GAP complex.
FEBS letters 2008;582(17):2501-7.
2008: S Meyer; A Scrima; W Versées; Alfred Wittinghofer
Crystal structures of the conserved tRNA-modifying enzyme GidA: implications for its interaction with MnmE and substrate.
Journal of molecular biology 2008;380(3):532-47.
2008: Nils Schrader; Carolin Koerner; Katja Koessmeier; Jan-Amadé Bangert; Alfred Wittinghofer; Raphael Stoll; Ingrid R Vetter
The crystal structure of the Ran-Nup153ZnF2 complex: a general Ran docking site at the nuclear pore complex.
Structure (London, England : 1993) 2008;16(7):1116-25.
2008: Raphael Gasper; Christoph Thomas; Mohammad Reza Ahmadian; Alfred Wittinghofer
The role of the conserved switch II glutamate in guanine nucleotide exchange factor-mediated nucleotide exchange of GTP-binding proteins.
Journal of molecular biology 2008;379(1):51-63.
2008: Carsten Kötting; Angela Kallenbach; Yan Suveyzdis; Alfred Wittinghofer; Klaus Gerwert
The GAP arginine finger movement into the catalytic site of Ras increases the activation entropy.
Proceedings of the National Academy of Sciences of the United States of America 2008;105(17):6260-5.
2008: Andrea Scrima; Christoph Thomas; Delia Deaconescu; Alfred Wittinghofer
The Rap-RapGAP complex: GTP hydrolysis without catalytic glutamine and arginine residues.
The EMBO journal 2008;27(7):1145-53.
2008: Stefan Veltel; Raphael Gasper; Elke Eisenacher; Alfred Wittinghofer
The retinitis pigmentosa 2 gene product is a GTPase-activating protein for Arf-like 3.
Nature structural & molecular biology 2008;15(4):373-80.
2008: Antje Berken; Alfred Wittinghofer
Structure and function of Rho-type molecular switches in plants.
Plant physiology and biochemistry : PPB / Société française de physiologie végétale 2008;46(3):380-93.
2007: Minhajuddin Sirajuddin; Marian Farkasovsky; Florian Hauer; Dorothee Kühlmann; Ian G Macara; Michael Weyand; Holger Stark; Alfred Wittinghofer
Structural insight into filament formation by mammalian septins.
Nature 2007;449(7160):311-5.
2007: Roland Koller-Eichhorn; Tobias Marquardt; Robert Gail; Alfred Wittinghofer; Dirk Kostrewa; Ulrike Kutay; Christian Kambach
Human OLA1 defines an ATPase subfamily in the Obg family of GTP-binding proteins.
The Journal of biological chemistry 2007;282(27):19928-37.
2007: Johannes L Bos; Holger Rehmann; Alfred Wittinghofer
GEFs and GAPs: critical elements in the control of small G proteins.
Cell 2007;129(5):865-77.
2007: Chakrabarti Partha; Oliver Daumke; Yan Suveyzdis; Carsten Kötting; Klaus Gerwert; Alfred Wittinghofer
Insight into catalysis of a unique GTPase reaction by a combined biochemical and FTIR approach.
Journal of molecular biology 2007;367(4):983-95.
2007: Christian Ottmann; Sergio Marco; Nina Jaspert; Caroline Marcon; Nicolas Schauer; Michael Weyand; Caroline Vandermeeren; Geoffrey Duby; Marc Boutry; Alfred Wittinghofer; Jean-Louis Rigaud; Claudia Oecking
Structure of a 14-3-3 coordinated hexamer of the plant plasma membrane H+ -ATPase by combining X-ray crystallography and electron cryomicroscopy.
Molecular cell 2007;25(3):427-40.
2007: Christian Ottmann; Lubna Yasmin; Michael Weyand; Jeffrey L Veesenmeyer; Maureen H Diaz; Ruth H Palmer; Matthew S Francis; Alan R Hauser; Alfred Wittinghofer; Bengt Hallberg
Phosphorylation-independent interaction between 14-3-3 and exoenzyme S: from structure to pathogenesis.
The EMBO journal 2007;26(3):902-13.
2007: Christoph Thomas; Inka Fricke; Andrea Scrima; Antje Berken; Alfred Wittinghofer
Structural evidence for a common intermediate in small G protein-GEF reactions.
Molecular cell 2007;25(1):141-9.
2007: Holger Rehmann; Alfred Wittinghofer; Johannes L Bos
Capturing cyclic nucleotides in action: snapshots from crystallographic studies.
Nature reviews. Molecular cell biology 2007;8(1):63-73.
2006: Luc Brunsveld; Jürgen Kuhlmann; Kirill Alexandrov; Alfred Wittinghofer; Roger S Goody; Herbert Waldmann
Lipidated ras and rab peptides and proteins--synthesis, structure, and function.
Angewandte Chemie (International ed. in English) 2006;45(40):6622-46.
2006: Carsten Kötting; Marco Blessenohl; Yan Suveyzdis; Roger S Goody; Alfred Wittinghofer; Klaus Gerwert
A phosphoryl transfer intermediate in the GTPase reaction of Ras in complex with its GTPase-activating protein.
Proceedings of the National Academy of Sciences of the United States of America 2006;103(38):13911-6.
2006: Raphael Gasper; Andrea Scrima; Alfred Wittinghofer
Structural insights into HypB, a GTP-binding protein that regulates metal binding.
The Journal of biological chemistry 2006;281(37):27492-502.
2006: Arjan Kortholt; Holger Rehmann; Helmut Kae; Leonard Bosgraaf; Ineke Keizer-Gunnink; Gerald Weeks; Alfred Wittinghofer; peter van haastert
Characterization of the GbpD-activated Rap1 pathway regulating adhesion and cell polarity in Dictyostelium discoideum.
The Journal of biological chemistry 2006;281(33):23367-76.
2006: Andrea Scrima; Alfred Wittinghofer
Dimerisation-dependent GTPase reaction of MnmE: how potassium acts as GTPase-activating element.
The EMBO journal 2006;25(12):2940-51.
2006: Christoph Thomas; Michael Weyand; Alfred Wittinghofer; Antje Berken
Purification and crystallization of the catalytic PRONE domain of RopGEF8 and its complex with Rop4 from Arabidopsis thaliana.
Acta crystallographica. Section F, Structural biology and crystallization communications 2006;62(Pt 6):607-10.
2006: Sabine Kupzig; Delia Deaconescu; Dalila Bouyoucef; Simon A Walker; Qing Liu; Christian L Polte; Oliver Daumke; Toshimasa Ishizaki; Peter J Lockyer; Alfred Wittinghofer; Peter J Cullen
GAP1 family members constitute bifunctional Ras and Rap GTPase-activating proteins.
The Journal of biological chemistry 2006;281(15):9891-900.
2006: Agnidipta Ghosh; Gerrit J K Praefcke; Louis Renault; Alfred Wittinghofer; Christian Herrmann
How guanylate-binding proteins achieve assembly-stimulated processive cleavage of GTP to GMP.
Nature 2006;440(7080):101-4.
2006: Holger Rehmann; Joost Das; Puck Knipscheer; Alfred Wittinghofer; Johannes L Bos
Structure of the cyclic-AMP-responsive exchange factor Epac2 in its auto-inhibited state.
Nature 2006;439(7076):625-8.
2006: Karin Kühnel; Stefan Veltel; Ilme Schlichting; Alfred Wittinghofer
Crystal structure of the human retinitis pigmentosa 2 protein and its interaction with Arl3.
Structure (London, England : 1993) 2006;14(2):367-78.
2006: Alfred Wittinghofer
Phosphoryl transfer in Ras proteins, conclusive or elusive?
Trends in biochemical sciences 2006;31(1):20-3.
2005: Michael Lammers; Rolf Rose; Andrea Scrima; Alfred Wittinghofer
The regulation of mDia1 by autoinhibition and its release by Rho*GTP.
The EMBO journal 2005;24(23):4176-87.
2005: Antje Berken; Christoph Thomas; Alfred Wittinghofer
A new family of RhoGEFs activates the Rop molecular switch in plants.
Nature 2005;436(7054):1176-80.
2005: Michael Hanzal-Bayer; Marco Linari; Alfred Wittinghofer
Properties of the interaction of Arf-like protein 2 with PDEdelta.
Journal of molecular biology 2005;350(5):1074-82.
2005: Marian Farkasovsky; Peter Herter; Beate Voss; Alfred Wittinghofer
Nucleotide binding and filament assembly of recombinant yeast septin complexes.
Biological chemistry 2005;386(7):643-56.
2005: Rolf Rose; Michael Weyand; M Lammers; T Ishizaki; Mohammad Reza Ahmadian; Alfred Wittinghofer
Structural and mechanistic insights into the interaction between Rho and mammalian Dia.
Nature 2005;435(7041):513-8.
2005: Oliver Rocks; Anna Peyker; Martin Kahms; Peter J Verveer; Carolin Koerner; Maria Lumbierres; Jürgen Kuhlmann; Herbert Waldmann; Alfred Wittinghofer; Philippe I H Bastiaens
An acylation cycle regulates localization and activity of palmitoylated Ras isoforms.
Science (New York, N.Y.) 2005;307(5716):1746-52.
2005: Robert Gail; Ronald Frank; Alfred Wittinghofer
Systematic peptide array-based delineation of the differential beta-catenin interaction with Tcf4, E-cadherin, and adenomatous polyposis coli.
The Journal of biological chemistry 2005;280(8):7107-17.
2005: Michael Spoerner; Andrea Nuehs; Petra Ganser; Christian Herrmann; Alfred Wittinghofer; Hans Robert Kalbitzer
Conformational states of Ras complexed with the GTP analogue GppNHp or GppCH2p: implications for the interaction with effector proteins.
Biochemistry 2005;44(6):2225-36.
2005: Rolf Rose; Alfred Wittinghofer; Michael Weyand
The purification and crystallization of mDia1 in complex with RhoC.
Acta crystallographica. Section F, Structural biology and crystallization communications 2005;61(Pt 2):225-7.
2005: Andrea Scrima; Ingrid R Vetter; M-Eugenia Armengod; Alfred Wittinghofer
The structure of the TrmE GTP-binding protein and its implications for tRNA modification.
The EMBO journal 2005;24(1):23-33.
2005: Michael Rossbach; Oliver Daumke; Claudia Klinger; Alfred Wittinghofer; Michael Kaufmann
Crystal structure of THEP1 from the hyperthermophile Aquifex aeolicus: a variation of the RecA fold.
BMC structural biology 2005;5():7.
2004: Michael Spoerner; Alfred Wittinghofer; Hans Robert Kalbitzer
Perturbation of the conformational equilibria in Ras by selective mutations as studied by 31P NMR spectroscopy.
FEBS letters 2004;578(3):305-10.
2004: Karin Kühnel; Thomas Jarchau; Eva Wolf; Ilme Schlichting; Ulrich Walter; Alfred Wittinghofer; Sergei V Strelkov
The VASP tetramerization domain is a right-handed coiled coil based on a 15-residue repeat.
Proceedings of the National Academy of Sciences of the United States of America 2004;101(49):17027-32.
2004: Chakrabarti Partha; Yan Suveyzdis; Alfred Wittinghofer; Klaus Gerwert
Fourier transform infrared spectroscopy on the Rap.RapGAP reaction, GTPase activation without an arginine finger.
The Journal of biological chemistry 2004;279(44):46226-33.
2004: Laurent Soulère; Courtney Aldrich; Oliver Daumke; Robert Gail; Lars Kissau; Alfred Wittinghofer; Herbert Waldmann
Synthesis of GTP-derived Ras ligands.
Chembiochem : a European journal of chemical biology 2004;5(10):1448-53.
2004: Oliver Daumke; Michael Weyand; Chakrabarti Partha; Ingrid R Vetter; Alfred Wittinghofer
The GTPase-activating protein Rap1GAP uses a catalytic asparagine.
Nature 2004;429(6988):197-201.
2004: Oliver Daumke; Alfred Wittinghofer; Michael Weyand
Purification, crystallization and preliminary structural characterization of human Rap1GAP.
Acta crystallographica. Section D, Biological crystallography 2004;60(Pt 4):752-4.
2004: Atsushi Shimada; Miklós Nyitrai; Ingrid R Vetter; Dorothee Kühlmann; Beáta Bugyi; Shuh Narumiya; Michael A Geeves; Alfred Wittinghofer
The core FH2 domain of diaphanous-related formins is an elongated actin binding protein that inhibits polymerization.
Molecular cell 2004;13(4):511-22.
2003: Michael J Seewald; Astrid Kraemer; Marian Farkasovsky; Carolin Körner; Alfred Wittinghofer; Ingrid R Vetter
Biochemical characterization of the Ran-RanBP1-RanGAP system: are RanBP proteins and the acidic tail of RanGAP required for the Ran-RanGAP GTPase reaction?
Molecular and cellular biology 2003;23(22):8124-36.
2003: Holger Rehmann; Frank Schwede; Stein Ove Døskeland; Alfred Wittinghofer; Johannes L Bos
Ligand-mediated activation of the cAMP-responsive guanine nucleotide exchange factor Epac.
The Journal of biological chemistry 2003;278(40):38548-56.
2003: Markus C Schlumberger; Andrea Friebel; Gretel Buchwald; Klaus Scheffzek; Alfred Wittinghofer; Wolf-Dietrich Hardt
Amino acids of the bacterial toxin SopE involved in G nucleotide exchange on Cdc42.
The Journal of biological chemistry 2003;278(29):27149-59.
2003: Holger Rehmann; Alma Rueppel; Johannes L Bos; Alfred Wittinghofer
Communication between the regulatory and the catalytic region of the cAMP-responsive guanine nucleotide exchange factor Epac.
The Journal of biological chemistry 2003;278(26):23508-14.
2003: Martin Würtele; Christian Jelich-Ottmann; Alfred Wittinghofer; Claudia Oecking
Structural view of a fungal toxin acting on a 14-3-3 regulatory complex.
The EMBO journal 2003;22(5):987-94.
2003: H Bea Kuiperij; Johan de Rooij; Holger Rehmann; Miranda van Triest; Alfred Wittinghofer; Johannes L Bos; Fried J T Zwartkruis
Characterisation of PDZ-GEFs, a family of guanine nucleotide exchange factors specific for Rap1 and Rap2.
Biochimica et biophysica acta 2003;1593(2-3):141-9.
2003: Holger Rehmann; Balaji Prakash; Eva Wolf; Alma Rueppel; Johan de Rooij; Johannes L Bos; Alfred Wittinghofer
Structure and regulation of the cAMP-binding domains of Epac2.
Nature structural biology 2003;10(1):26-32.
2002: Astrid Kraemer; Thilo Brinkmann; Ina Plettner; Roger S Goody; Alfred Wittinghofer
Fluorescently labelled guanine nucleotide binding proteins to analyse elementary steps of GAP-catalysed reactions.
Journal of molecular biology 2002;324(4):763-74.
2002: Alfred Wittinghofer
Obg, a G domain with a beautiful extension.
Structure (London, England : 1993) 2002;10(11):1471-2.
2002: Gretel Buchwald; Andrea Friebel; Jorge E Galán; Wolf-Dietrich Hardt; Alfred Wittinghofer; Klaus Scheffzek
Structural basis for the reversible activation of a Rho protein by the bacterial toxin SopE.
The EMBO journal 2002;21(13):3286-95.
2002: Michael Hanzal-Bayer; Louis Renault; Pietro Roversi; Alfred Wittinghofer; Roman C Hillig
The complex of Arl2-GTP and PDE delta: from structure to function.
The EMBO journal 2002;21(9):2095-106.
2002: Thilo Brinkmann; Oliver Daumke; Ulrike Herbrand; Dorothee Kühlmann; Patricia Stege; Mohammad Reza Ahmadian; Alfred Wittinghofer
Rap-specific GTPase activating protein follows an alternative mechanism.
The Journal of biological chemistry 2002;277(15):12525-31.
2002: Mohammad Reza Ahmadian; Alfred Wittinghofer; Gudula Schmidt
The actin filament architecture: tightly regulated by the cells, manipulated by pathogens. International Titisee Conference on the actin cytoskeleton: from signalling to bacterial pathogenesis.
EMBO reports 2002;3(3):214-8.
2002: Michael J Seewald; Carolin Körner; Alfred Wittinghofer; Ingrid R Vetter
RanGAP mediates GTP hydrolysis without an arginine finger.
Nature 2002;415(6872):662-6.
2002: Mohammad Reza Ahmadian; Alfred Wittinghofer; Christian Herrmann
Fluorescence methods in the study of small GTP-binding proteins.
Methods in molecular biology (Clifton, N.J.) 2002;189():45-63.
2001: Ingrid R Vetter; Alfred Wittinghofer
The guanine nucleotide-binding switch in three dimensions.
Science (New York, N.Y.) 2001;294(5545):1299-304.
2001: Klaus Scheffzek; P Grünewald; Sabine Wohlgemuth; W Kabsch; H Tu; Michael Wigler; Alfred Wittinghofer; Christian Herrmann
The Ras-Byr2RBD complex: structural basis for Ras effector recognition in yeast.
Structure (London, England : 1993) 2001;9(11):1043-50.
2001: R Gail; B Costisella; Mohammad Reza Ahmadian; Alfred Wittinghofer
Ras-mediated cleavage of a GTP analogue by a novel mechanism.
Chembiochem : a European journal of chemical biology 2001;2(7-8):570-5.
2001: Gretel Buchwald; E Hostinova; M G Rudolph; A Kraemer; A Sickmann; H E Meyer; Klaus Scheffzek; Alfred Wittinghofer
Conformational switch and role of phosphorylation in PAK activation.
Molecular and cellular biology 2001;21(15):5179-89.
2001: C Allin; Mohammad Reza Ahmadian; Alfred Wittinghofer; Klaus Gerwert
Monitoring the GAP catalyzed H-Ras GTPase reaction at atomic resolution in real time.
Proceedings of the National Academy of Sciences of the United States of America 2001;98(14):7754-9.
2001: MG Rudolph; Thomas Linnemann; P Grunewald; Alfred Wittinghofer; Ingrid R Vetter; Christian Herrmann
Thermodynamics of Ras/effector and Cdc42/effector interactions probed by isothermal titration calorimetry.
The Journal of biological chemistry 2001;276(26):23914-21.
2001: Michael Spoerner; Christian Herrmann; Ingrid R Vetter; Hans Robert Kalbitzer; Alfred Wittinghofer
Dynamic properties of the Ras switch I region and its importance for binding to effectors.
Proceedings of the National Academy of Sciences of the United States of America 2001;98(9):4944-9.
2001: Louis Renault; Jürgen Kuhlmann; A Henkel; Alfred Wittinghofer
Structural basis for guanine nucleotide exchange on Ran by the regulator of chromosome condensation (RCC1).
Cell 2001;105(2):245-55.
2001: A Kraemer; Holger Rehmann; Robbert Cool; C Theiss; Johan de Rooij; Johannes L Bos; Alfred Wittinghofer
Dynamic interaction of cAMP with the Rap guanine-nucleotide exchange factor Epac1.
Journal of molecular biology 2001;306(5):1167-77.
2001: M Würtele; Louis Renault; Joseph T Barbieri; Alfred Wittinghofer; E Wolf
Structure of the ExoS GTPase activating domain.
FEBS letters 2001;491(1-2):26-9.
2001: M Rohrer; Thomas F Prisner; O Brügmann; H Käss; Michael Spoerner; Alfred Wittinghofer; Hans Robert Kalbitzer
Structure of the metal-water complex in Ras x GDP studied by high-field EPR spectroscopy and 31P NMR spectroscopy.
Biochemistry 2001;40(7):1884-9.
2001: M Würtele; E Wolf; Kristin J Pederson; G Buchwald; Mohammad Reza Ahmadian; Joseph T Barbieri; Alfred Wittinghofer
How the Pseudomonas aeruginosa ExoS toxin downregulates Rac.
Nature structural biology 2001;8(1):23-6.
2000: Roman C Hillig; Michael Hanzal-Bayer; Marco Linari; J Becker; Alfred Wittinghofer; Louis Renault
Structural and biochemical properties show ARL3-GDP as a distinct GTP binding protein.
Structure (London, England : 1993) 2000;8(12):1239-45.
2000: B Klockow; Mohammad Reza Ahmadian; C Block; Alfred Wittinghofer
Oncogenic insertional mutations in the P-loop of Ras are overactive in MAP kinase signaling.
Oncogene 2000;19(47):5367-76.
2000: C I Villa Braslavsky; Christine Nowak; Dirk Görlich; Alfred Wittinghofer; Jürgen Kuhlmann
Different structural and kinetic requirements for the interaction of Ran with the Ran-binding domains from RanBP2 and importin-beta.
Biochemistry 2000;39(38):11629-39.
2000: Kim M T de Bruyn; Johan de Rooij; RM Wolthuis; Holger Rehmann; J Wesenbeek; Robbert Cool; Alfred Wittinghofer; Johannes L Bos
RalGEF2, a pleckstrin homology domain containing guanine nucleotide exchange factor for Ral.
The Journal of biological chemistry 2000;275(38):29761-6.
2000: Balaji Prakash; Louis Renault; Gerrit J K Praefcke; Christian Herrmann; Alfred Wittinghofer
Triphosphate structure of guanylate-binding protein 1 and implications for nucleotide binding and GTPase mechanism.
The EMBO journal 2000;19(17):4555-64.
2000: Johan de Rooij; Holger Rehmann; Miranda van Triest; Robbert Cool; Alfred Wittinghofer; Johannes L Bos
Mechanism of regulation of the Epac family of cAMP-dependent RapGEFs.
The Journal of biological chemistry 2000;275(27):20829-36.
2000: G Schmidt; Alfred Wittinghofer
Priming of PC12 cells for semiquantitative microinjection studies involving Ras.
FEBS letters 2000;474(2-3):184-8.
2000: F Huber; Wolfram Gronwald; Sabine Wohlgemuth; Christian Herrmann; Matthias Geyer; Alfred Wittinghofer; Hans Robert Kalbitzer
Sequential NMR assignment of the RAS-binding domain of Byr2.
Journal of biomolecular NMR 2000;16(4):355-6.
2000: Balaji Prakash; Gerrit J K Praefcke; Louis Renault; Alfred Wittinghofer; Christian Herrmann
Structure of human guanylate-binding protein 1 representing a unique class of GTP-binding proteins.
Nature 2000;403(6769):567-71.
2000: B Bader; K Kuhn; D J Owen; H Waldmann; Alfred Wittinghofer; J Kuhlmann
Bioorganic synthesis of lipid-modified proteins for the study of signal transduction.
Nature 2000;403(6766):223-6.
1999: Johan de Rooij; N M Boenink; Miranda van Triest; Robbert Cool; Alfred Wittinghofer; Johannes L Bos
PDZ-GEF1, a guanine nucleotide exchange factor specific for Rap1 and Rap2.
The Journal of biological chemistry 1999;274(53):38125-30.
1999: Robbert Cool; G Schmidt; C U Lenzen; Heino Prinz; D Vogt; Alfred Wittinghofer
The Ras mutant D119N is both dominant negative and activated.
Molecular and cellular biology 1999;19(9):6297-305.
1999: B Bauer; Gladys Mirey; Ingrid R Vetter; J A García-Ranea; Carmen Galvez; Alfred Wittinghofer; Jacques Camonis; Robbert Cool
Effector recognition by the small GTP-binding proteins Ras and Ral.
The Journal of biological chemistry 1999;274(25):17763-70.
1999: Mohammad Reza Ahmadian; T Zor; D Vogt; W Kabsch; Zvi Selinger; Alfred Wittinghofer; Klaus Scheffzek
Guanosine triphosphatase stimulation of oncogenic Ras mutants.
Proceedings of the National Academy of Sciences of the United States of America 1999;96(12):7065-70.
1999: Roman C Hillig; Louis Renault; Ingrid R Vetter; T Drell; Alfred Wittinghofer; J Becker
The crystal structure of rna1p: a new fold for a GTPase-activating protein.
Molecular cell 1999;3(6):781-91.
1999: Ingrid R Vetter; A Arndt; Ulrike Kutay; Dirk Görlich; Alfred Wittinghofer
Structural view of the Ran-Importin beta interaction at 2.3 A resolution.
Cell 1999;97(5):635-46.
1999: Ingrid R Vetter; Thomas Linnemann; Sabine Wohlgemuth; Matthias Geyer; Hans Robert Kalbitzer; Christian Herrmann; Alfred Wittinghofer
Structural and biochemical analysis of Ras-effector signaling via RalGDS.
FEBS letters 1999;451(2):175-80.
1999: Thomas Linnemann; Matthias Geyer; B K Jaitner; C Block; Hans Robert Kalbitzer; Alfred Wittinghofer; Christian Herrmann
Thermodynamic and kinetic characterization of the interaction between the Ras binding domain of AF6 and members of the Ras subfamily.
The Journal of biological chemistry 1999;274(19):13556-62.
1999: N van den Berghe; Robbert Cool; Alfred Wittinghofer
Discriminatory residues in Ras and Rap for guanine nucleotide exchange factor recognition.
The Journal of biological chemistry 1999;274(16):11078-85.
1999: MG Rudolph; Alfred Wittinghofer; Ingrid R Vetter
Nucleotide binding to the G12V-mutant of Cdc42 investigated by X-ray diffraction and fluorescence spectroscopy: two different nucleotide states in one crystal.
Protein science : a publication of the Protein Society 1999;8(4):778-87.
1999: Ingrid R Vetter; Christine Nowak; T Nishimoto; Jürgen Kuhlmann; Alfred Wittinghofer
Structure of a Ran-binding domain complexed with Ran bound to a GTP analogue: implications for nuclear transport.
Nature 1999;398(6722):39-46.
1999: Peter Herter; Cornelius Kuhnen; Klaus-Michael Müller; Alfred Wittinghofer; Oliver Müller
Intracellular distribution of beta-catenin in colorectal adenomas, carcinomas and Peutz-Jeghers polyps.
Journal of cancer research and clinical oncology 1999;125(5):297-304.
1999: Louis Renault; N Nassar; Alfred Wittinghofer; M Roth; Ingrid R Vetter
Crystallization and preliminary X-ray analysis of human RCC1, the regulator of chromosome condensation.
Acta crystallographica. Section D, Biological crystallography 1999;55(Pt 1):272-5.
1998: Johan de Rooij; Fried J T Zwartkruis; M H G Verheijen; Robbert Cool; S M Nijman; Alfred Wittinghofer; Johannes L Bos
Epac is a Rap1 guanine-nucleotide-exchange factor directly activated by cyclic AMP.
Nature 1998;396(6710):474-7.
1998: M Daub; J Jöckel; T Quack; Christoph K Weber; F Schmitz; Ulf R Rapp; Alfred Wittinghofer; C Block
The RafC1 cysteine-rich domain contains multiple distinct regulatory epitopes which control Ras-dependent Raf activation.
Molecular and cellular biology 1998;18(11):6698-710.
1998: D Esser; B Bauer; RM Wolthuis; Alfred Wittinghofer; Robbert Cool; P Bayer
Structure determination of the Ras-binding domain of the Ral-specific guanine nucleotide exchange factor Rlf.
Biochemistry 1998;37(39):13453-62.
1998: Klaus Scheffzek; Mohammad Reza Ahmadian; L Wiesmüller; W Kabsch; Patricia Stege; F Schmitz; Alfred Wittinghofer
Structural analysis of the GAP-related domain from neurofibromin and its implications.
The EMBO journal 1998;17(15):4313-27.
1998: Alfred Wittinghofer
Signal transduction via Ras.
Biological chemistry 1998;379(8-9):933-7.
1998: MG Rudolph; P Bayer; A Abo; J Kuhlmann; Ingrid R Vetter; Alfred Wittinghofer
The Cdc42/Rac interactive binding region motif of the Wiskott Aldrich syndrome protein (WASP) is necessary but not sufficient for tight binding to Cdc42 and structure formation.
The Journal of biological chemistry 1998;273(29):18067-76.
1998: Klaus Scheffzek; Mohammad Reza Ahmadian; Alfred Wittinghofer
GTPase-activating proteins: helping hands to complement an active site.
Trends in biochemical sciences 1998;23(7):257-62.
1998: C Lenzen; Robbert Cool; Heino Prinz; Jürgen Kuhlmann; Alfred Wittinghofer
Kinetic analysis by fluorescence of the interaction between Ras and the catalytic domain of the guanine nucleotide exchange factor Cdc25Mm.
Biochemistry 1998;37(20):7420-30.
1998: Louis Renault; N Nassar; Ingrid R Vetter; J Becker; C Klebe; M Roth; Alfred Wittinghofer
The 1.7 A crystal structure of the regulator of chromosome condensation (RCC1) reveals a seven-bladed propeller.
Nature 1998;392(6671):97-101.
1997: B K Jaitner; J Becker; Thomas Linnemann; Christian Herrmann; Alfred Wittinghofer; C Block
Discrimination of amino acids mediating Ras binding from noninteracting residues affecting raf activation by double mutant analysis.
The Journal of biological chemistry 1997;272(47):29927-33.
1997: Alfred Wittinghofer
Signaling mechanistics: aluminum fluoride for molecule of the year.
Current biology : CB 1997;7(11):R682-5.
1997: Jürgen Kuhlmann; Ian G Macara; Alfred Wittinghofer
Dynamic and equilibrium studies on the interaction of Ran with its effector, RanBP1.
Biochemistry 1997;36(40):12027-35.
1997: Matthias Geyer; Christian Herrmann; Sabine Wohlgemuth; Alfred Wittinghofer; Hans Robert Kalbitzer
Structure of the Ras-binding domain of RalGEF and implications for Ras binding and signalling.
Nature structural biology 1997;4(9):694-9.
1997: Mohammad Reza Ahmadian; Patricia Stege; Klaus Scheffzek; Alfred Wittinghofer
Confirmation of the arginine-finger hypothesis for the GAP-stimulated GTP-hydrolysis reaction of Ras.
Nature structural biology 1997;4(9):686-9.
1997: N van den Berghe; Robbert Cool; G Horn; Alfred Wittinghofer
Biochemical characterization of C3G: an exchange factor that discriminates between Rap1 and Rap2 and is not inhibited by Rap1A(S17N).
Oncogene 1997;15(7):845-50.
1997: Klaus Scheffzek; Mohammad Reza Ahmadian; W Kabsch; L Wiesmüller; A Lautwein; F Schmitz; Alfred Wittinghofer
The Ras-RasGAP complex: structural basis for GTPase activation and its loss in oncogenic Ras mutants.
Science (New York, N.Y.) 1997;277(5324):333-8.
1997: Alfred Wittinghofer; Klaus Scheffzek; Mohammad Reza Ahmadian
The interaction of Ras with GTPase-activating proteins.
FEBS letters 1997;410(1):63-7.
1997: Mohammad Reza Ahmadian; R Mittal; Alan Hall; Alfred Wittinghofer
Aluminum fluoride associates with the small guanine nucleotide binding proteins.
FEBS letters 1997;408(3):315-8.
1997: Mohammad Reza Ahmadian; U Hoffmann; Roger S Goody; Alfred Wittinghofer
Individual rate constants for the interaction of Ras proteins with GTPase-activating proteins determined by fluorescence spectroscopy.
Biochemistry 1997;36(15):4535-41.
1997: T Schweins; Klaus Scheffzek; R Assheuer; Alfred Wittinghofer
The role of the metal ion in the p21ras catalysed GTP-hydrolysis: Mn2+ versus Mg2+.
Journal of molecular biology 1997;266(4):847-56.
1997: Klaus Scheffzek; A Lautwein; A Scherer; S Franken; Alfred Wittinghofer
Crystallization and preliminary X-ray crystallographic study of the Ras-GTPase-activating domain of human p120GAP.
Proteins 1997;27(2):315-8.
1996: Klaus Scheffzek; A Lautwein; W Kabsch; Mohammad Reza Ahmadian; Alfred Wittinghofer
Crystal structure of the GTPase-activating domain of human p120GAP and implications for the interaction with Ras.
Nature 1996;384(6609):591-6.
1996: Alfred Wittinghofer; N Nassar
How Ras-related proteins talk to their effectors.
Trends in biochemical sciences 1996;21(12):488-91.
1996: T Schweins; Matthias Geyer; Hans Robert Kalbitzer; Alfred Wittinghofer; Arieh Warshel
Linear free energy relationships in the intrinsic and GTPase activating protein-stimulated guanosine 5'-triphosphate hydrolysis of p21ras.
Biochemistry 1996;35(45):14225-31.
1996: Matthias Geyer; T Schweins; Christian Herrmann; T Prisner; Alfred Wittinghofer; Hans Robert Kalbitzer
Conformational transitions in p21ras and in its complexes with the effector protein Raf-RBD and the GTPase activating protein GAP.
Biochemistry 1996;35(32):10308-20.
1996: N Nassar; G Horn; Christian Herrmann; C Block; R Janknecht; Alfred Wittinghofer
Ras/Rap effector specificity determined by charge reversal.
Nature structural biology 1996;3(8):723-9.
1996: RM Wolthuis; B Bauer; L J van 't Veer; Alida M M de Vries-Smits; Robbert Cool; M Spaargaren; Alfred Wittinghofer; Boudewijn M T Burgering; Johannes L Bos
RalGDS-like factor (Rlf) is a novel Ras and Rap 1A-associating protein.
Oncogene 1996;13(2):353-62.
1996: Mohammad Reza Ahmadian; L Wiesmüller; A Lautwein; F R Bischoff; Alfred Wittinghofer
Structural differences in the minimal catalytic domains of the GTPase-activating proteins p120GAP and neurofibromin.
The Journal of biological chemistry 1996;271(27):16409-15.
1996: R Mittal; Mohammad Reza Ahmadian; Roger S Goody; Alfred Wittinghofer
Formation of a transition-state analog of the Ras GTPase reaction by Ras-GDP, tetrafluoroaluminate, and GTPase-activating proteins.
Science (New York, N.Y.) 1996;273(5271):115-7.
1996: Christian Herrmann; G Horn; M Spaargaren; Alfred Wittinghofer
Differential interaction of the ras family GTP-binding proteins H-Ras, Rap1A, and R-Ras with the putative effector molecules Raf kinase and Ral-guanine nucleotide exchange factor.
The Journal of biological chemistry 1996;271(12):6794-800.
1996: C Block; R Janknecht; Christian Herrmann; N Nassar; Alfred Wittinghofer
Quantitative structure-activity analysis correlating Ras/Raf interaction in vitro to Raf activation in vivo.
Nature structural biology 1996;3(3):244-51.
1996: G Schmidt; C Lenzen; I Simon; R Deuter; Robbert Cool; Roger S Goody; Alfred Wittinghofer
Biochemical and biological consequences of changing the specificity of p21ras from guanosine to xanthosine nucleotides.
Oncogene 1996;12(1):87-96.
1995: C Block; Alfred Wittinghofer
Switching to Rac and Rho.
Structure (London, England : 1993) 1995;3(12):1281-4.
1995: Axel J Scheidig; S M Franken; J E Corrie; Gordon P Reid; Alfred Wittinghofer; Emil F Pai; Roger S Goody
X-ray crystal structure analysis of the catalytic domain of the oncogene product p21H-ras complexed with caged GTP and mant dGppNHp.
Journal of molecular biology 1995;253(1):132-50.
1995: C Klebe; Heino Prinz; Alfred Wittinghofer; Roger S Goody
The kinetic mechanism of Ran--nucleotide exchange catalyzed by RCC1.
Biochemistry 1995;34(39):12543-52.
1995: Alfred Wittinghofer; Christian Herrmann
Ras-effector interactions, the problem of specificity.
FEBS letters 1995;369(1):52-6.
1995: N Nassar; G Horn; Christian Herrmann; A Scherer; F McCormick; Alfred Wittinghofer
The 2.2 A crystal structure of the Ras-binding domain of the serine/threonine kinase c-Raf1 in complex with Rap1A and a GTP analogue.
Nature 1995;375(6532):554-60.
1995: J Becker; F Melchior; Volker Gerke; F Ralf Bischoff; Herwig Ponstingl; Alfred Wittinghofer
RNA1 encodes a GTPase-activating protein specific for Gsp1p, the Ran/TC4 homologue of Saccharomyces cerevisiae.
The Journal of biological chemistry 1995;270(20):11860-5.
1995: L Wiesmüller; Klaus Scheffzek; Werner Kliche; Roger S Goody; Alfred Wittinghofer; Jochen Reinstein
Crystallization and preliminary X-ray analysis of UMP/CMP-kinase from Dictyostelium discoideum with the specific bisubstrate inhibitor P1-(adenosine 5')-P5-(uridine 5')-pentaphosphate (UP5A).
FEBS letters 1995;363(1-2):22-4.
1995: Klaus Scheffzek; C Klebe; Karin Fritz-Wolf; W Kabsch; Alfred Wittinghofer
Crystal structure of the nuclear Ras-related protein Ran in its GDP-bound form.
Nature 1995;374(6520):378-81.
1995: Christian Herrmann; George Martin; Alfred Wittinghofer
Quantitative analysis of the complex between p21ras and the Ras-binding domain of the human Raf-1 protein kinase.
The Journal of biological chemistry 1995;270(7):2901-5.
1995: C Klebe; F Ralf Bischoff; Herwig Ponstingl; Alfred Wittinghofer
Interaction of the nuclear GTP-binding protein Ran with its regulatory proteins RCC1 and RanGAP1.
Biochemistry 1995;34(2):639-47.
1995: H Rensland; J John; R Linke; I Simon; Ilme Schlichting; Alfred Wittinghofer; Roger S Goody
Substrate and product structural requirements for binding of nucleotides to H-ras p21: the mechanism of discrimination between guanosine and adenosine nucleotides.
Biochemistry 1995;34(2):593-9.
1995: C Lenzen; Robbert Cool; Alfred Wittinghofer
Analysis of intrinsic and CDC25-stimulated guanine nucleotide exchange of p21ras-nucleotide complexes by fluorescence measurements.
Methods in enzymology 1995;255():95-109.
1995: T Schweins; Matthias Geyer; Klaus Scheffzek; Arieh Warshel; Hans Robert Kalbitzer; Alfred Wittinghofer
Substrate-assisted catalysis as a mechanism for GTP hydrolysis of p21ras and other GTP-binding proteins.
Nature structural biology 1995;2(1):36-44.
1994: Axel J Scheidig; A Sanchez-Llorente; A Lautwein; Emil F Pai; J E Corrie; Gordon P Reid; Alfred Wittinghofer; Roger S Goody
Crystallographic studies on p21(H-ras) using the synchrotron Laue method: improvement of crystal quality and monitoring of the GTPase reaction at different time points.
Acta crystallographica. Section D, Biological crystallography 1994;50(Pt 4):512-20.
1994: T Schweins; Alfred Wittinghofer
GTP-binding proteins. Structures, interactions and relationships.
Current biology : CB 1994;4(6):547-50.
1994: F Ralf Bischoff; C Klebe; J Kretschmer; Alfred Wittinghofer; Herwig Ponstingl
RanGAP1 induces GTPase activity of nuclear Ras-related Ran.
Proceedings of the National Academy of Sciences of the United States of America 1994;91(7):2587-91.
1994: M Frech; Thomas A Darden; Lee G Pedersen; C K Foley; P S Charifson; M W Anderson; Alfred Wittinghofer
Role of glutamine-61 in the hydrolysis of GTP by p21H-ras: an experimental and theoretical study.
Biochemistry 1994;33(11):3237-44.
1994: Alfred Wittinghofer
The structure of transducin G alpha t: more to view than just ras.
Cell 1994;76(2):201-4.
1993: Alfred Wittinghofer
From EF-Tu to p21ras and back again.
Current biology : CB 1993;3(12):874-6.
1993: J John; H Rensland; Ilme Schlichting; Ingrid R Vetter; G D Borasio; Roger S Goody; Alfred Wittinghofer
Kinetic and structural analysis of the Mg(2+)-binding site of the guanine nucleotide-binding protein p21H-ras.
The Journal of biological chemistry 1993;268(2):923-9.
1993: Alfred Wittinghofer; S M Franken; Axel J Scheidig; H Rensland; A Lautwein; Emil F Pai; Roger S Goody
Three-dimensional structure and properties of wild-type and mutant H-ras-encoded p21.
Ciba Foundation symposium 1993;176():6-21; discussion 21-7.
1992: V Wolber; K Maeda; R Schumann; B Brandmeier; L Wiesmüller; Alfred Wittinghofer
A universal expression-purification system based on the coiled-coil interaction of myosin heavy chain.
Bio/technology (Nature Publishing Company) 1992;10(8):900-4.
1992: Jürgen Serth; W Weber; M Frech; Alfred Wittinghofer; Alfred Pingoud
Binding of the H-ras p21 GTPase activating protein by the activated epidermal growth factor receptor leads to inhibition of the p21 GTPase activity in vitro.
Biochemistry 1992;31(28):6361-5.
1992: M Y Mistou; E Jacquet; P Poullet; H Rensland; P Gideon; Ilme Schlichting; Alfred Wittinghofer; Andrea Parmeggiani
Mutations of Ha-ras p21 that define important regions for the molecular mechanism of the SDC25 C-domain, a guanine nucleotide dissociation stimulator.
The EMBO journal 1992;11(7):2391-7.
1992: O Müller; M Frech; P Gideon; Alfred Wittinghofer; Michael Schwarz
Differences in GTPase-activating protein activity between liver tumors and normal liver tissue in mice.
Oncogene 1992;7(7):1407-12.
1992: C K Foley; Lee G Pedersen; P S Charifson; Thomas A Darden; Alfred Wittinghofer; E F Pai; M W Anderson
Simulation of the solution structure of the H-ras p21-GTP complex.
Biochemistry 1992;31(21):4951-9.
1992: L Wiesmüller; Alfred Wittinghofer
Expression of the GTPase activating domain of the neurofibromatosis type 1 (NF1) gene in Escherichia coli and role of the conserved lysine residue.
The Journal of biological chemistry 1992;267(15):10207-10.
1992: P Gideon; J John; M Frech; A Lautwein; R Clark; J E Scheffler; Alfred Wittinghofer
Mutational and kinetic analyses of the GTPase-activating protein (GAP)-p21 interaction: the C-terminal domain of GAP is not sufficient for full activity.
Molecular and cellular biology 1992;12(5):2050-6.
1992: V Wolber; H Rensland; B Brandmeier; M Sagemann; R Hoffmann; Hans Robert Kalbitzer; Alfred Wittinghofer
Expression, purification and biochemical characterisation of the human immunodeficiency virus 1 nef gene product.
European journal of biochemistry / FEBS 1992;205(3):1115-21.
1992: Roger S Goody; Emil F Pai; Ilme Schlichting; H Rensland; Axel J Scheidig; S Franken; Alfred Wittinghofer
Studies on the structure and mechanism of H-ras p21.
Philosophical transactions of the Royal Society of London. Series B, Biological sciences 1992;336(1276):3-10; discussion 10-1.
1991: H Rensland; A Lautwein; Alfred Wittinghofer; Roger S Goody
Is there a rate-limiting step before GTP cleavage by H-ras p21?
Biochemistry 1991;30(46):11181-5.
1991: Alfred Wittinghofer; Emil F Pai
The structure of Ras protein: a model for a universal molecular switch.
Trends in biochemical sciences 1991;16(10):382-7.
1991: Jochen Reinstein; Ilme Schlichting; M Frech; Roger S Goody; Alfred Wittinghofer
p21 with a phenylalanine 28----leucine mutation reacts normally with the GTPase activating protein GAP but nevertheless has transforming properties.
The Journal of biological chemistry 1991;266(26):17700-6.
1991: Hans Robert Kalbitzer; K Maeda; A Rösch; Yuichiro Maéda; Matthias Geyer; W Beneicke; K P Neidig; Alfred Wittinghofer
C-terminal structure and mobility of rabbit skeletal muscle light meromyosin as studied by one- and two-dimensional 1H NMR spectroscopy and X-ray small-angle scattering.
Biochemistry 1991;30(32):8083-91.
1991: Hans Robert Kalbitzer; Alfred Wittinghofer
The effect of kirromycin on the metal ion coordination in complexes of elongation factor Tu from Bacillus stearothermophilus as inferred from the 17O-55Mn superhyperfine interaction.
Biochimica et biophysica acta 1991;1078(2):133-8.
1991: Jürgen Serth; A Lautwein; M Frech; Alfred Wittinghofer; Alfred Pingoud
The inhibition of the GTPase activating protein-Ha-ras interaction by acidic lipids is due to physical association of the C-terminal domain of the GTPase activating protein with micellar structures.
The EMBO journal 1991;10(6):1325-30.
1991: K Maeda; A Rösch; Yuichiro Maéda; Hans Robert Kalbitzer; Alfred Wittinghofer
Rabbit skeletal muscle myosin. Unfolded carboxyl-terminus and its role in molecular assembly.
FEBS letters 1991;281(1-2):23-6.
1991: I Bock-Möbius; M Brune; Alfred Wittinghofer; H Zimmermann; R Leberman; M T Dauvergne; S Zimmermann; B Brandmeier; Paul Rösch
Identification of valine/leucine/isoleucine and threonine/alanine/glycine proton-spin systems of Escherichia coli adenylate kinase by selective deuteration and selective protonation.
The Biochemical journal 1991;273(Pt 2)():311-6.
1990: Jochen Reinstein; Ilme Schlichting; Alfred Wittinghofer
Structurally and catalytically important residues in the phosphate binding loop of adenylate kinase of Escherichia coli.
Biochemistry 1990;29(32):7451-9.
1990: Jochen Reinstein; Ingrid R Vetter; Ilme Schlichting; Paul Rösch; Alfred Wittinghofer; Roger S Goody
Fluorescence and NMR investigations on the ligand binding properties of adenylate kinases.
Biochemistry 1990;29(32):7440-50.
1990: U Krengel; Ilme Schlichting; A Scherer; R Schumann; M Frech; J John; W Kabsch; Emil F Pai; Alfred Wittinghofer
Three-dimensional structures of H-ras p21 mutants: molecular basis for their inability to function as signal switch molecules.
Cell 1990;62(3):539-48.
1990: Emil F Pai; U Krengel; G A Petsko; Roger S Goody; W Kabsch; Alfred Wittinghofer
Refined crystal structure of the triphosphate conformation of H-ras p21 at 1.35 A resolution: implications for the mechanism of GTP hydrolysis.
The EMBO journal 1990;9(8):2351-9.
1990: M Frech; J John; V Pizon; Pierre Chardin; A Tavitian; R Clark; F McCormick; Alfred Wittinghofer
Inhibition of GTPase activating protein stimulation of Ras-p21 GTPase by the Krev-1 gene product.
Science (New York, N.Y.) 1990;249(4965):169-71.
1990: J John; R Sohmen; Juergen Feuerstein; R Linke; Alfred Wittinghofer; Roger S Goody
Kinetics of interaction of nucleotides with nucleotide-free H-ras p21.
Biochemistry 1990;29(25):6058-65.
1990: Ilme Schlichting; Steven C Almo; G Rapp; K Wilson; K Petratos; A Lentfer; Alfred Wittinghofer; W Kabsch; Emil F Pai; G A Petsko
Time-resolved X-ray crystallographic study of the conformational change in Ha-Ras p21 protein on GTP hydrolysis.
Nature 1990;345(6273):309-15.
1990: M Frech; Ilme Schlichting; Alfred Wittinghofer; Pierre Chardin
Guanine nucleotide binding properties of the mammalian RalA protein produced in Escherichia coli.
The Journal of biological chemistry 1990;265(11):6353-9.
1990: Hans Robert Kalbitzer; Juergen Feuerstein; Roger S Goody; Alfred Wittinghofer
Stereochemistry and lifetime of the GTP hydrolysis intermediate at the active site of elongation factor Tu from Bacillus stearothermophilus as inferred from the 17O-55Mn superhyperfine interaction.
European journal of biochemistry / FEBS 1990;188(2):355-9.
1990: Ilme Schlichting; J John; M Frech; Pierre Chardin; Alfred Wittinghofer; H Zimmermann; Paul Rösch
Proton NMR studies of transforming and nontransforming H-ras p21 mutants.
Biochemistry 1990;29(2):504-11.
1989: Ilme Schlichting; G Rapp; J John; Alfred Wittinghofer; Emil F Pai; Roger S Goody
Biochemical and crystallographic characterization of a complex of c-Ha-ras p21 and caged GTP with flash photolysis.
Proceedings of the National Academy of Sciences of the United States of America 1989;86(20):7687-90.
1989: Emil F Pai; W Kabsch; U Krengel; Kenneth C Holmes; J John; Alfred Wittinghofer
Structure of the guanine-nucleotide-binding domain of the Ha-ras oncogene product p21 in the triphosphate conformation.
Nature 1989;341(6239):209-14.
1989: J John; Ilme Schlichting; E Schiltz; Paul Rösch; Alfred Wittinghofer
C-terminal truncation of p21H preserves crucial kinetic and structural properties.
The Journal of biological chemistry 1989;264(22):13086-92.
1989: G H Haase; M Brune; Jochen Reinstein; Emil F Pai; Alfred Pingoud; Alfred Wittinghofer
Adenylate kinases from thermosensitive Escherichia coli strains.
Journal of molecular biology 1989;207(1):151-62.
1988: Alfred Pingoud; M Wehrmann; Uwe Pieper; Frank-Ulrich Gast; Claus Urbanke; Jürgen Alves; Juergen Feuerstein; Alfred Wittinghofer
Spectroscopic and hydrodynamic studies reveal structural differences in normal and transforming H-ras gene products.
Biochemistry 1988;27(13):4735-40.
1988: Jochen Reinstein; M Brune; Alfred Wittinghofer
Mutations in the nucleotide binding loop of adenylate kinase of Escherichia coli.
Biochemistry 1988;27(13):4712-20.
1988: I Bock; Jochen Reinstein; M Brune; Alfred Wittinghofer; Paul Rösch
Proton-nuclear magnetic resonance studies of the aromatic spin systems of Escherichia coli adenylate kinase.
Journal of molecular biology 1988;200(4):745-8.
1988: Ilme Schlichting; Alfred Wittinghofer; Paul Rösch
Proton NMR studies of the GDP.Mg2+ complex of the Ha-ras oncogene product p21.
Biochemical and biophysical research communications 1988;150(1):444-8.
1987: Juergen Feuerstein; Hans Robert Kalbitzer; J John; Roger S Goody; Alfred Wittinghofer
Characterisation of the metal-ion-GDP complex at the active sites of transforming and nontransforming p21 proteins by observation of the 17O-Mn superhyperfine coupling and by kinetic methods.
European journal of biochemistry / FEBS 1987;162(1):49-55.