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Richard Brimacombe

This is a preview profile on BiomedExperts - the first literature-based scientific social network. It brings the right researchers together and allows them to collaborate online. Collexis and Dell provide the BiomedExperts network of +1.5 Million pre-calculated profiles free of charge to researchers worldwide.

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Living Beings

Escherichia coli

Anatomy

Ribosomes

Concepts & Ideas

Chemicals & Drugs

Phenomena

Ultraviolet Rays

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Co-Publications
Name
20
Dontsova, Olga
14
Rinke-Appel, Jutta
12
Mueller, Florian
11
Sergiev, Petr
10
Bogdanov, Alexey
9
Bogdanov, Alexey
9
Dokudovskaya, SS
8
van Heel, Marin
6
Stöffler, G
5
Baranov, Pavel
5
Stark, Holger
5
Leonov, Andrei
4
Osswald, Monika
3
Matadeen, R
3
Orlova, Elena

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Publications

TOP 3
Sergiev Petr V; Lesnyak Dmitry V; Burakovsky Dmitry E; Kiparisov Sergey V; Leonov Andrei A; Bogdanov Alexey A; Brimacombe Richard; Dontsova Olga A
Alteration in location of a conserved GTPase-associated center of the ribosome induced by mutagenesis influences the structure of peptidyltransferase center and activity of elongation factor G.
Sergiev Petr V; Lesnyak Dmitry V; Kiparisov Sergey V; Burakovsky Dmitry E; Leonov Andrei A; Bogdanov Alexey A; Brimacombe Richard; Dontsova Olga A
Function of the ribosomal E-site: a mutagenesis study.
Leonov Andrei A; Sergiev Petr V; Bogdanov Alexey A; Brimacombe Richard; Dontsova Olga A
Affinity purification of ribosomes with a lethal G2655C mutation in 23 S rRNA that affects the translocation.

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All Publications

2005: Sergiev Petr V; Lesnyak Dmitry V; Burakovsky Dmitry E; Kiparisov Sergey V; Leonov Andrei A; Bogdanov Alexey A; Brimacombe Richard; Dontsova Olga A
Alteration in location of a conserved GTPase-associated center of the ribosome induced by mutagenesis influences the structure of peptidyltransferase center and activity of elongation factor G.
The Journal of biological chemistry 2005;280(36):31882-9.
2005: Sergiev Petr V; Lesnyak Dmitry V; Kiparisov Sergey V; Burakovsky Dmitry E; Leonov Andrei A; Bogdanov Alexey A; Brimacombe Richard; Dontsova Olga A
Function of the ribosomal E-site: a mutagenesis study.
Nucleic acids research 2005;33(18):6048-56.
2003: Leonov Andrei A; Sergiev Petr V; Bogdanov Alexey A; Brimacombe Richard; Dontsova Olga A
Affinity purification of ribosomes with a lethal G2655C mutation in 23 S rRNA that affects the translocation.
The Journal of biological chemistry 2003;278(28):25664-70.
2002: Rinke-Appel Jutta; Osswald Monika; von Knoblauch Klaus; Mueller Florian; Brimacombe Richard; Sergiev Petr; Avdeeva Olga; Bogdanov Alexey; Dontsova Olga
Crosslinking of 4.5S RNA to the Escherichia coli ribosome in the presence or absence of the protein Ffh.
RNA (New York, N.Y.) 2002;8(5):612-25.
2002: Avdeeva Olga N; Myasnikov Alexander G; Sergiev Petr V; Bogdanov Alexey A; Brimacombe Richard; Dontsova Olga A
Construction of the 'minimal' SRP that interacts with the translating ribosome but not with specific membrane receptors in Escherichia coli.
FEBS letters 2002;514(1):70-3.
2001: Kubarenko A V; Sergiev P V; Bogdanov A A; Brimacombe R; Dontsova O A
A protonated base pair participating in rRNA tertiary structural interactions.
Nucleic acids research 2001;29(24):5067-70.
2001: Moll I; Huber M; Grill S; Sairafi P; Mueller F; Brimacombe R; Londei P; Bläsi U
Evidence against an Interaction between the mRNA downstream box and 16S rRNA in translation initiation.
Journal of bacteriology 2001;183(11):3499-505.
2001: Matadeen R; Sergiev P; Leonov A; Pape T; van der Sluis E; Mueller F; Osswald M; von Knoblauch K; Brimacombe R; Bogdanov A; van Heel M; Dontsova O
Direct localization by cryo-electron microscopy of secondary structural elements in Escherichia coli 23 S rRNA which differ from the corresponding regions in Haloarcula marismortui.
Journal of molecular biology 2001;307(5):1341-9.
2001: Sergiev P; Leonov A; Dokudovskaya S; Shpanchenko O; Dontsova O; Bogdanov A; Rinke-Appel J; Mueller F; Osswald M; von Knoblauch K; Brimacombe R
Correlating the X-ray structures for halo- and thermophilic ribosomal subunits with biochemical data for the Escherichia coli ribosome.
Cold Spring Harbor symposia on quantitative biology 2001;66():87-100.
2000: Mueller F; Sommer I; Baranov P; Matadeen R; Stoldt M; Wöhnert J; Görlach M; van Heel M; Brimacombe R
The 3D arrangement of the 23 S and 5 S rRNA in the Escherichia coli 50 S ribosomal subunit based on a cryo-electron microscopic reconstruction at 7.5 A resolution.
Journal of molecular biology 2000;298(1):35-59.
1999: Matadeen R; Patwardhan A; Gowen B; Orlova E V; Pape T; Cuff M; Mueller F; Brimacombe R; van Heel M
The Escherichia coli large ribosomal subunit at 7.5 A resolution.
Structure (London, England : 1993) 1999;7(12):1575-83.
1999: Osswald M; Brimacombe R
The environment of 5S rRNA in the ribosome: cross-links to 23S rRNA from sites within helices II and III of the 5S molecule.
Nucleic acids research 1999;27(11):2283-90.
1999: Baranov P V; Kubarenko A V; Gurvich O L; Shamolina T A; Brimacombe R
The Database of Ribosomal Cross-links: an update.
Nucleic acids research 1999;27(1):184-5.
1998: Choi K M; Atkins J F; Gesteland R F; Brimacombe R
Flexibility of the nascent polypeptide chain within the ribosome--contacts from the peptide N-terminus to a specific region of the 30S subunit.
European journal of biochemistry / FEBS 1998;255(2):409-13.
1998: Dube P; Bacher G; Stark H; Mueller F; Zemlin F; van Heel M; Brimacombe R
Correlation of the expansion segments in mammalian rRNA with the fine structure of the 80 S ribosome; a cryoelectron microscopic reconstruction of the rabbit reticulocyte ribosome at 21 A resolution.
Journal of molecular biology 1998;279(2):403-21.
1998: Baranov P V; Gurvich O L; Bogdanov A A; Brimacombe R; Dontsova O A
New features of 23S ribosomal RNA folding: the long helix 41-42 makes a "U-turn" inside the ribosome.
RNA (New York, N.Y.) 1998;4(6):658-68.
1998: Sergiev P; Dokudovskaya S; Romanova E; Topin A; Bogdanov A; Brimacombe R; Dontsova O
The environment of 5S rRNA in the ribosome: cross-links to the GTPase-associated area of 23S rRNA.
Nucleic acids research 1998;26(11):2519-25.
1998: Tanaka I; Nakagawa A; Hosaka H; Wakatsuki S; Mueller F; Brimacombe R
Matching the crystallographic structure of ribosomal protein S7 to a three-dimensional model of the 16S ribosomal RNA.
RNA (New York, N.Y.) 1998;4(5):542-50.
1998: Choi K M; Brimacombe R
The path of the growing peptide chain through the 23S rRNA in the 50S ribosomal subunit; a comparative cross-linking study with three different peptide families.
Nucleic acids research 1998;26(4):887-95.
1998: Baranov P V; Sergiev P V; Dontsova O A; Bogdanov A A; Brimacombe R
The Database of Ribosomal Cross links (DRC).
Nucleic acids research 1998;26(1):187-9.
1997: Stark H; Rodnina M V; Rinke-Appel J; Brimacombe R; Wintermeyer W; van Heel M
Visualization of elongation factor Tu on the Escherichia coli ribosome.
Nature 1997;389(6649):403-6.
1997: Mueller F; Stark H; van Heel M; Rinke-Appel J; Brimacombe R
A new model for the three-dimensional folding of Escherichia coli 16 S ribosomal RNA. III. The topography of the functional centre.
Journal of molecular biology 1997;271(4):566-87.
1997: Mueller F; Brimacombe R
A new model for the three-dimensional folding of Escherichia coli 16 S ribosomal RNA. II. The RNA-protein interaction data.
Journal of molecular biology 1997;271(4):545-65.
1997: Mueller F; Brimacombe R
A new model for the three-dimensional folding of Escherichia coli 16 S ribosomal RNA. I. Fitting the RNA to a 3D electron microscopic map at 20 A.
Journal of molecular biology 1997;271(4):524-44.
1997: Baranov P V; Dokudovskaya S S; Oretskaya T S; Dontsova O A; Bogdanov A A; Brimacombe R
A new technique for the characterization of long-range tertiary contacts in large RNA molecules: insertion of a photolabel at a selected position in 16S rRNA within the Escherichia coli ribosome.
Nucleic acids research 1997;25(12):2266-73.
1997: Sergiev P V; Lavrik I N; Wlasoff V A; Dokudovskaya S S; Dontsova O A; Bogdanov A A; Brimacombe R
The path of mRNA through the bacterial ribosome: a site-directed crosslinking study using new photoreactive derivatives of guanosine and uridine.
RNA (New York, N.Y.) 1997;3(5):464-75.
1997: Stark H; Orlova E V; Rinke-Appel J; Jünke N; Mueller F; Rodnina M; Wintermeyer W; Brimacombe R; van Heel M
Arrangement of tRNAs in pre- and posttranslocational ribosomes revealed by electron cryomicroscopy.
Cell 1997;88(1):19-28.
1996: Dokudovskaya S; Dontsova O; Shpanchenko O; Bogdanov A; Brimacombe R
Loop IV of 5S ribosomal RNA has contacts both to domain II and to domain V of the 23S RNA.
RNA (New York, N.Y.) 1996;2(2):146-52.
1995: Rinke-Appel J; Jünke N; Osswald M; Brimacombe R
The ribosomal environment of tRNA: crosslinks to rRNA from positions 8 and 20:1 in the central fold of tRNA located at the A, P, or E site.
RNA (New York, N.Y.) 1995;1(10):1018-28.
1995: Mueller F; Döring T; Erdemir T; Greuer B; Jünke N; Osswald M; Rinke-Appel J; Stade K; Thamm S; Brimacombe R
Getting closer to an understanding of the three-dimensional structure of ribosomal RNA.
Biochemistry and cell biology = Biochimie et biologie cellulaire 1995;73(11-12):767-73.
1995: Osswald M; Döring T; Brimacombe R
The ribosomal neighbourhood of the central fold of tRNA: cross-links from position 47 of tRNA located at the A, P or E site.
Nucleic acids research 1995;23(22):4635-41.
1995: Stark H; Mueller F; Orlova E V; Schatz M; Dube P; Erdemir T; Zemlin F; Brimacombe R; van Heel M
The 70S Escherichia coli ribosome at 23 A resolution: fitting the ribosomal RNA.
Structure (London, England : 1993) 1995;3(8):815-21.
1995: Stade K; Jünke N; Brimacombe R
Mapping the path of the nascent peptide chain through the 23S RNA in the 50S ribosomal subunit.
Nucleic acids research 1995;23(13):2371-80.
1995: Brimacombe R
The structure of ribosomal RNA: a three-dimensional jigsaw puzzle.
European journal of biochemistry / FEBS 1995;230(2):365-83.
1994: Rinke-Appel J; Jünke N; Brimacombe R; Lavrik I; Dokudovskaya S; Dontsova O; Bogdanov A
Contacts between 16S ribosomal RNA and mRNA, within the spacer region separating the AUG initiator codon and the Shine-Dalgarno sequence; a site-directed cross-linking study.
Nucleic acids research 1994;22(15):3018-25.
1994: Döring T; Mitchell P; Osswald M; Bochkariov D; Brimacombe R
The decoding region of 16S RNA; a cross-linking study of the ribosomal A, P and E sites using tRNA derivatized at position 32 in the anticodon loop.
The EMBO journal 1994;13(11):2677-85.
1994: Dontsova O; Tishkov V; Dokudovskaya S; Bogdanov A; Döring T; Rinke-Appel J; Thamm S; Greuer B; Brimacombe R
Stem-loop IV of 5S rRNA lies close to the peptidyltransferase center.
Proceedings of the National Academy of Sciences of the United States of America 1994;91(10):4125-9.
1994: Stade K; Riens S; Bochkariov D; Brimacombe R
Contacts between the growing peptide chain and the 23S RNA in the 50S ribosomal subunit.
Nucleic acids research 1994;22(8):1394-9.
1993: Dokudovskaya S S; Dontsova O A; Bogdanova S L; Bogdanov A A; Brimacombe R
mRNA-ribosome interactions.
Biotechnology and applied biochemistry 1993;18 ( Pt 2)():149-55.
1993: Rinke-Appel J; Jünke N; Brimacombe R; Dukudovskaya S; Dontsova O; Bogdanov A
Site-directed cross-linking of mRNA analogues to 16S ribosomal RNA; a complete scan of cross-links from all positions between '+1' and '+16' on the mRNA, downstream from the decoding site.
Nucleic acids research 1993;21(12):2853-9.
1993: Mitchell P; Stade K; Osswald M; Brimacombe R
Site-directed cross-linking studies on the E. coli tRNA-ribosome complex: determination of sites labelled with an aromatic azide attached to the variable loop or aminoacyl group of tRNA.
Nucleic acids research 1993;21(4):887-96.
1993: Brimacombe R; Mitchell P; Osswald M; Stade K; Bochkariov D
Clustering of modified nucleotides at the functional center of bacterial ribosomal RNA.
The FASEB journal : official publication of the Federation of American Societies for Experimental Biology 1993;7(1):161-7.
1992: Dontsova O; Dokudovskaya S; Kopylov A; Bogdanov A; Rinke-Appel J; Jünke N; Brimacombe R
Three widely separated positions in the 16S RNA lie in or close to the ribosomal decoding region; a site-directed cross-linking study with mRNA analogues.
The EMBO journal 1992;11(8):3105-16.
1992: Brimacombe R
Structure-function correlations (and discrepancies) in the 16S ribosomal RNA from Escherichia coli.
Biochimie 1992;74(4):319-26.
1992: Döring T; Greuer B; Brimacombe R
The topography of the 3'-terminal region of Escherichia coli 16S ribosomal RNA; an intra-RNA cross-linking study.
Nucleic acids research 1992;20(7):1593-7.
1992: Mitchell P; Osswald M; Brimacombe R
Identification of intermolecular RNA cross-links at the subunit interface of the Escherichia coli ribosome.
Biochemistry 1992;31(11):3004-11.
1991: Dontsova O; Kopylov A; Brimacombe R
The location of mRNA in the ribosomal 30S initiation complex; site-directed cross-linking of mRNA analogues carrying several photo-reactive labels simultaneously on either side of the AUG start codon.
The EMBO journal 1991;10(9):2613-20.
1991: Rinke-Appel J; Jünke N; Stade K; Brimacombe R
The path of mRNA through the Escherichia coli ribosome; site-directed cross-linking of mRNA analogues carrying a photo-reactive label at various points 3' to the decoding site.
The EMBO journal 1991;10(8):2195-202.
1991: Brimacombe R
RNA-protein interactions in the Escherichia coli ribosome.
Biochimie 1991;73(7-8):927-36.
1991: Döring T; Greuer B; Brimacombe R
The three-dimensional folding of ribosomal RNA; localization of a series of intra-RNA cross-links in 23S RNA induced by treatment of Escherichia coli 50S ribosomal subunits with bis-(2-chloroethyl)-methylamine.
Nucleic acids research 1991;19(13):3517-24.
1990: Osswald M; Greuer B; Brimacombe R
Localization of a series of RNA-protein cross-link sites in the 23S and 5S ribosomal RNA from Escherichia coli, induced by treatment of 50S subunits with three different bifunctional reagents.
Nucleic acids research 1990;18(23):6755-60.
1990: Tate W; Greuer B; Brimacombe R
Codon recognition in polypeptide chain termination: site directed crosslinking of termination codon to Escherichia coli release factor 2.
Nucleic acids research 1990;18(22):6537-44.
1990: Brimacombe R; Gornicki P; Greuer B; Mitchell P; Osswald M; Rinke-Appel J; Schüler D; Stade K
The three-dimensional structure and function of Escherichia coli ribosomal RNA, as studied by cross-linking techniques.
Biochimica et biophysica acta 1990;1050(1-3):8-13.
1990: Mitchell P; Osswald M; Schueler D; Brimacombe R
Selective isolation and detailed analysis of intra-RNA cross-links induced in the large ribosomal subunit of E. coli: a model for the tertiary structure of the tRNA binding domain in 23S RNA.
Nucleic acids research 1990;18(15):4325-33.
1989: Stade K; Rinke-Appel J; Brimacombe R
Site-directed cross-linking of mRNA analogues to the Escherichia coli ribosome; identification of 30S ribosomal components that can be cross-linked to the mRNA at various points 5' with respect to the decoding site.
Nucleic acids research 1989;17(23):9889-908.
1988: Walleczek J; Schüler D; Stöffler-Meilicke M; Brimacombe R; Stöffler G
A model for the spatial arrangement of the proteins in the large subunit of the Escherichia coli ribosome.
The EMBO journal 1988;7(11):3571-6.
1988: Brimacombe R
The emerging three-dimensional structure and function of 16S ribosomal RNA.
Biochemistry 1988;27(12):4207-14.
1988: Stiege W; Kosack M; Stade K; Brimacombe R
Intra-RNA cross-linking in Escherichia coli 30S ribosomal subunits: selective isolation of cross-linked products by hybridization to specific cDNA fragments.
Nucleic acids research 1988;16(10):4315-29.
1988: Schüler D; Brimacombe R
The Escherichia coli 30S ribosomal subunit; an optimized three-dimensional fit between the ribosomal proteins and the 16S RNA.
The EMBO journal 1988;7(5):1509-13.
1988: Stiege W; Stade K; Schüler D; Brimacombe R
Covalent cross-linking of poly(A) to Escherichia coli ribosomes, and localization of the cross-link site within the 16S RNA.
Nucleic acids research 1988;16(6):2369-88.
1988: Wiener L; Schüler D; Brimacombe R
Protein binding sites on Escherichia coli 16S ribosomal RNA; RNA regions that are protected by proteins S7, S9 and S19, and by proteins S8, S15 and S17.
Nucleic acids research 1988;16(4):1233-50.
1988: Gulle H; Hoppe E; Osswald M; Greuer B; Brimacombe R; Stöffler G
RNA-protein cross-linking in Escherichia coli 50S ribosomal subunits; determination of sites on 23S RNA that are cross-linked to proteins L2, L4, L24 and L27 by treatment with 2-iminothiolane.
Nucleic acids research 1988;16(3):815-32.
1988: Brimacombe R; Atmadja J; Stiege W; Schüler D
A detailed model of the three-dimensional structure of Escherichia coli 16 S ribosomal RNA in situ in the 30 S subunit.
Journal of molecular biology 1988;199(1):115-36.
1988: Brimacombe R; Stiege W; Kyriatsoulis A; Maly P
Intra-RNA and RNA-protein cross-linking techniques in Escherichia coli ribosomes.
Methods in enzymology 1988;164():287-309.
1987: Gulle H; Brimacombe R; Stöffler-Meilicke M; Stöffler G
A rapid immunological spot test for the identification of proteins in covalently linked protein-nucleic acid complexes.
Journal of immunological methods 1987;102(2):183-6.
1987: Greuer B; Osswald M; Brimacombe R; Stöffler G
RNA-protein cross-linking in Escherichia coli 30S ribosomal subunits; determination of sites on 16S RNA that are cross-linked to proteins S3, S4, S7, S9, S10, S11, S17, S18 and S21 by treatment with bis-(2-chloroethyl)-methylamine.
Nucleic acids research 1987;15(8):3241-55.
1987: Osswald M; Greuer B; Brimacombe R; Stöffler G; Bäumert H; Fasold H
RNA-protein cross-linking in Escherichia coli 30S ribosomal subunits; determination of sites on 16S RNA that are cross-linked to proteins S3, S4, S5, S7, S8, S9, S11, S13, S19 and S21 by treatment with methyl p-azidophenyl acetimidate.
Nucleic acids research 1987;15(8):3221-40.
1986: Stiege W; Atmadja J; Zobawa M; Brimacombe R
Investigation of the tertiary folding of Escherichia coli ribosomal RNA by intra-RNA cross-linking in vivo.
Journal of molecular biology 1986;191(1):135-8.
1986: Kyriatsoulis A; Maly P; Greuer B; Brimacombe R; Stöffler G; Frank R; Blöcker H
RNA-protein cross-linking in Escherichia coli ribosomal subunits: localization of sites on 16S RNA which are cross-linked to proteins S17 and S21 by treatment with 2-iminothiolane.
Nucleic acids research 1986;14(3):1171-86.
1986: Atmadja J; Stiege W; Zobawa M; Greuer B; Osswald M; Brimacombe R
The tertiary folding of Escherichia coli 16S RNA, as studied by in situ intra-RNA cross-linking of 30S ribosomal subunits with bis-(2-chloroethyl)-methylamine.
Nucleic acids research 1986;14(2):659-73.
1985: Atmadja J; Brimacombe R; Blöcker H; Frank R
Investigation of the tertiary folding of Escherichia coli 16S RNA by in situ intra-RNA cross-linking within 30S ribosomal subunits.
Nucleic acids research 1985;13(19):6919-36.
1984: Atmadja J; Brimacombe R; Maden B E
Xenopus laevis 18S ribosomal RNA: experimental determination of secondary structural elements, and locations of methyl groups in the secondary structure model.
Nucleic acids research 1984;12(6):2649-67.
1983: Maly P; Wower J; Zobawa M; Brimacombe R
Identification of tyrosine residues that are susceptible to lactoperoxidase-catalyzed iodination on the surface of Escherichia coli 50s ribosomal subunits or 70s ribosomes.
Biochemistry 1983;22(13):3157-62.
1983: Wower J; Maly P; Zobawa M; Brimacombe R
Identification of tyrosine residues that are susceptible to lactoperoxidase-catalyzed iodination on the surface of Escherichia coli 30S ribosomal subunit.
Biochemistry 1983;22(10):2339-46.
1983: Stiege W; Glotz C; Brimacombe R
Localisation of a series of intra-RNA cross-links in the secondary and tertiary structure of 23S RNA, induced by ultraviolet irradiation of Escherichia coli 50S ribosomal subunits.
Nucleic acids research 1983;11(6):1687-706.
1983: Wower I; Brimacombe R
The localization of multiple sites on 16S RNA which are cross-linked to proteins S7 and S8 in Escherichia coli 30S ribosomal subunits by treatment with 2-iminothiolane.
Nucleic acids research 1983;11(5):1419-37.
1982: Stiege W; Zwieb C; Brimacombe R
Precise localisation of three intra-RNA cross-links in 23S RNA and one in 5S RNA, induced by treatment of Escherichia coli 50S ribosomal subunits with bis-(2-chloroethyl)-methylamine.
Nucleic acids research 1982;10(22):7211-29.
1981: Wower I; Wower J; Meinke M; Brimacombe R
The use of 2-iminothiolane as an RNA-protein cross-linking agent in Escherichia coli ribosomes, and the localisation on 23S RNA of sites cross-linked to proteins L4, L6, L21, L23, L27 and L29.
Nucleic acids research 1981;9(17):4285-302.
1981: Zwieb C; Glotz C; Brimacombe R
Secondary structure comparisons between small subunit ribosomal RNA molecules from six different species.
Nucleic acids research 1981;9(15):3621-40.
1981: Glotz C; Zwieb C; Brimacombe R; Edwards K; Kössel H
Secondary structure of the large subunit ribosomal RNA from Escherichia coli, Zea mays chloroplast, and human and mouse mitochondrial ribosomes.
Nucleic acids research 1981;9(14):3287-306.
1980: Glotz C; Brimacombe R
An experimentally-derived model for the secondary structure of the 16S ribosomal RNA from Escherichia coli.
Nucleic acids research 1980;8(11):2377-95.

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