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Tohru Yoshimura

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Daisuke Sasaki; Masahiro Fujihashi; Yuki Iwata; Motomichi Murakami; Tohru Yoshimura; Hisashi Hemmi; Kunio Miki
Structure and mutation analysis of archaeal geranylgeranyl reductase.
Takae Yamauchi; Masaru Goto; Hui-Yuan Wu; Takuma Uo; Tohru Yoshimura; Hisaaki Mihara; Tatsuo Kurihara; Ikuko Miyahara; Ken Hirotsu; Nobuyoshi Esaki
Serine racemase with catalytically active lysinoalanyl residue.
Geok-Yong Yow; Takuma Uo; Tohru Yoshimura; Nobuyoshi Esaki
Physiological role of D-amino acid-N-acetyltransferase of Saccharomyces cerevisiae: detoxification of D-amino acids.

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2011: Daisuke Sasaki; Masahiro Fujihashi; Yuki Iwata; Motomichi Murakami; Tohru Yoshimura; Hisashi Hemmi; Kunio Miki
Structure and mutation analysis of archaeal geranylgeranyl reductase.
Journal of molecular biology 2011;409(4):543-57.
2009: Takae Yamauchi; Masaru Goto; Hui-Yuan Wu; Takuma Uo; Tohru Yoshimura; Hisaaki Mihara; Tatsuo Kurihara; Ikuko Miyahara; Ken Hirotsu; Nobuyoshi Esaki
Serine racemase with catalytically active lysinoalanyl residue.
Journal of biochemistry 2009;145(4):421-4.
2006: Geok-Yong Yow; Takuma Uo; Tohru Yoshimura; Nobuyoshi Esaki
Physiological role of D-amino acid-N-acetyltransferase of Saccharomyces cerevisiae: detoxification of D-amino acids.
Archives of microbiology 2006;185(1):39-46.
2005: Kazuaki Yoshimune; Andrey Galkin; Liudmila Kulakova; Tohru Yoshimura; Nobuyoshi Esaki
Cold-active DnaK of an Antarctic psychrotroph Shewanella sp. Ac10 supporting the growth of dnaK-null mutant of Escherichia coli at cold temperatures.
Extremophiles : life under extreme conditions 2005;9(2):145-50.
2005: Kazuaki Yoshimune; Andrey Galkin; Liudmila Kulakova; Tohru Yoshimura; Nobuyoshi Esaki
DnaK from Vibrio proteolyticus: complementation of a dnaK-null mutant of Escherichia coli and the role of its ATPase domain.
Journal of bioscience and bioengineering 2005;99(2):136-42.
2004: Geok-Yong Yow; Takuma Uo; Tohru Yoshimura; Nobuyoshi Esaki
D-amino acid N-acetyltransferase of Saccharomyces cerevisiae: a close homologue of histone acetyltransferase Hpa2p acting exclusively on free D-amino acids.
Archives of microbiology 2004;182(5):396-403.
2003: Tatsuo Kurihara; Hisaaki Mihara; Shin-ichiro Kato; Tohru Yoshimura; Nobuyoshi Esaki
Assembly of iron-sulfur clusters mediated by cysteine desulfurases, IscS, CsdB and CSD, from Escherichia coli.
Biochimica et biophysica acta 2003;1647(1-2):303-9.
2003: Tohru Yoshimura; Nobuyoshi Esak
Amino acid racemases: functions and mechanisms.
Journal of bioscience and bioengineering 2003;96(2):103-9.
2002: Takuma Uo; Tohru Yoshimura; Tozo Nishiyama; Nobuyoshi Esaki
Gene cloning, purification, and characterization of 2,3-diaminopropionate ammonia-lyase from Escherichia coli.
Bioscience, biotechnology, and biochemistry 2002;66(12):2639-44.
2002: Kazuaki Yoshimune; Tohru Yoshimura; Toru Nakayama; Tokuzo Nishino; Nobuyoshi Esaki
Hsc62, Hsc56, and GrpE, the third Hsp70 chaperone system of Escherichia coli.
Biochemical and biophysical research communications 2002;293(5):1389-95.
2002: Akira Watanabe; Tohru Yoshimura; Bunzo Mikami; Hideyuki Hayashi; Hiroyuki Kagamiyama; Nobuyoshi Esaki
Reaction mechanism of alanine racemase from Bacillus stearothermophilus: x-ray crystallographic studies of the enzyme bound with N-(5'-phosphopyridoxyl)alanine.
The Journal of biological chemistry 2002;277(21):19166-72.
2002: Shin-ichiro Kato; Hisaaki Mihara; Tatsuo Kurihara; Yasuhiro Takahashi; Umechiyo Tokumoto; Tohru Yoshimura; Nobuyoshi Esaki
Cys-328 of IscS and Cys-63 of IscU are the sites of disulfide bridge formation in a covalently bound IscS/IscU complex: implications for the mechanism of iron-sulfur cluster assembly.
Proceedings of the National Academy of Sciences of the United States of America 2002;99(9):5948-52.
2001: Takuma Uo; Tohru Yoshimura; N Tanaka; K Takegawa; Nobuyoshi Esaki
Functional characterization of alanine racemase from Schizosaccharomyces pombe: a eucaryotic counterpart to bacterial alanine racemase.
Journal of bacteriology 2001;183(7):2226-33.
2000: A Gutierrez; Tohru Yoshimura; Yoshihiro Fuchikami; Nobuyoshi Esaki
Modulation of activity and substrate specificity by modifying the backbone length of the distant interdomain loop of D-amino acid aminotransferase.
European journal of biochemistry / FEBS 2000;267(24):7218-23.
2000: Shin-ichiro Kato; Hisaaki Mihara; Tatsuo Kurihara; Tohru Yoshimura; Nobuyoshi Esaki
Gene cloning, purification, and characterization of two cyanobacterial NifS homologs driving iron-sulfur cluster formation.
Bioscience, biotechnology, and biochemistry 2000;64(11):2412-9.
2000: Kwang-Hwan Jhee; Tohru Yoshimura; Edith Wilson Miles; S Takeda; Ikuko Miyahara; Ken Hirotsu; K Soda; Y Kawata; Nobuyoshi Esaki
Stereochemistry of the transamination reaction catalyzed by aminodeoxychorismate lyase from Escherichia coli: close relationship between fold type and stereochemistry.
Journal of biochemistry 2000;128(4):679-86.
2000: T Nakai; H Mizutani; Ikuko Miyahara; Ken Hirotsu; S Takeda; Kwang-Hwan Jhee; Tohru Yoshimura; Nobuyoshi Esaki
Three-dimensional structure of 4-amino-4-deoxychorismate lyase from Escherichia coli.
Journal of biochemistry 2000;128(1):29-38.
2000: Hisaaki Mihara; Tatsuo Kurihara; Tohru Yoshimura; Nobuyoshi Esaki
Kinetic and mutational studies of three NifS homologs from Escherichia coli: mechanistic difference between L-cysteine desulfurase and L-selenocysteine lyase reactions.
Journal of biochemistry 2000;127(4):559-67.
2000: Hisaaki Mihara; Tatsuo Kurihara; Takeshi Watanabe; Tohru Yoshimura; Nobuyoshi Esaki
cDNA cloning, purification, and characterization of mouse liver selenocysteine lyase. Candidate for selenium delivery protein in selenoprotein synthesis.
The Journal of biological chemistry 2000;275(9):6195-200.
1999: Akira Watanabe; Tohru Yoshimura; Bunzo Mikami; Nobuyoshi Esaki
Tyrosine 265 of alanine racemase serves as a base abstracting alpha-hydrogen from L-alanine: the counterpart residue to lysine 39 specific to D-alanine.
Journal of biochemistry 1999;126(4):781-6.
1999: Akira Watanabe; Y Kurokawa; Tohru Yoshimura; Nobuyoshi Esaki
Role of tyrosine 265 of alanine racemase from Bacillus stearothermophilus.
Journal of biochemistry 1999;125(6):987-90.
1999: Akira Watanabe; Y Kurokawa; Tohru Yoshimura; Tatsuo Kurihara; K Soda; Nobuyoshi Esaki; A Watababe
Role of lysine 39 of alanine racemase from Bacillus stearothermophilus that binds pyridoxal 5'-phosphate. Chemical rescue studies of Lys39 --> Ala mutant.
The Journal of biological chemistry 1999;274(7):4189-94.
1999: Liudmila Kulakova; Andrey Galkin; Tatsuo Kurihara; Tohru Yoshimura; Nobuyoshi Esaki
Cold-active serine alkaline protease from the psychrotrophic bacterium Shewanella strain ac10: gene cloning and enzyme purification and characterization.
Applied and environmental microbiology 1999;65(2):611-7.
1998: Y Kurokawa; Akira Watanabe; Tohru Yoshimura; Nobuyoshi Esaki; K Soda
Transamination as a side-reaction catalyzed by alanine racemase of Bacillus stearothermophilus.
Journal of biochemistry 1998;124(6):1163-9.
1998: Yoshihiro Fuchikami; Tohru Yoshimura; A Gutierrez; K Soda; Nobuyoshi Esaki
Construction and properties of a fragmentary D-amino acid aminotransferase.
Journal of biochemistry 1998;124(5):905-10.
1998: Kazuaki Yoshimune; Tohru Yoshimura; Nobuyoshi Esaki
Hsc62, a new DnaK homologue of Escherichia coli.
Biochemical and biophysical research communications 1998;250(1):115-8.
1998: Takuma Uo; Tohru Yoshimura; Sakayu Shimizu; Nobuyoshi Esaki
Occurrence of pyridoxal 5'-phosphate-dependent serine racemase in silkworm, Bombyx mori.
Biochemical and biophysical research communications 1998;246(1):31-4.
1998: Y H Lim; Tohru Yoshimura; Y Kurokawa; Nobuyoshi Esaki; K Soda
Nonstereospecific transamination catalyzed by pyridoxal phosphate-dependent amino acid racemases of broad substrate specificity.
The Journal of biological chemistry 1998;273(7):4001-5.
1998: A Gutierrez; Tohru Yoshimura; Yoshihiro Fuchikami; K Soda; Nobuyoshi Esaki
A mutant D-amino acid aminotransferase with broad substrate specificity: construction by replacement of the interdomain loop Pro119-Arg120-Pro121 by Gly-Gly-Gly.
Protein engineering 1998;11(1):53-8.
1998: Lijun Liu; Tohru Yoshimura; K Endo; Keiko Kishimoto; Yoshihiro Fuchikami; J M Manning; Nobuyoshi Esaki; K Soda
Compensation for D-glutamate auxotrophy of Escherichia coli WM335 by D-amino acid aminotransferase gene and regulation of murI expression.
Bioscience, biotechnology, and biochemistry 1998;62(1):193-5.
1997: K Kishimoto; Tohru Yoshimura; K Soda; Nobuyoshi Esaki
Mutation of arginine 98, which serves as a substrate-recognition site of D-amino acid aminotransferase, can be partly compensated for by mutation of tyrosine 88 to an arginyl residue.
Journal of biochemistry 1997;122(6):1182-9.
1997: Andrey Galkin; Liudmila Kulakova; Tohru Yoshimura; K Soda; Nobuyoshi Esaki
Synthesis of optically active amino acids from alpha-keto acids with Escherichia coli cells expressing heterologous genes.
Applied and environmental microbiology 1997;63(12):4651-6.
1997: Hisaaki Mihara; Tatsuo Kurihara; Tohru Yoshimura; K Soda; Nobuyoshi Esaki
Cysteine sulfinate desulfinase, a NIFS-like protein of Escherichia coli with selenocysteine lyase and cysteine desulfurase activities. Gene cloning, purification, and characterization of a novel pyridoxal enzyme.
The Journal of biological chemistry 1997;272(36):22417-24.
1997: Lijun Liu; Tohru Yoshimura; K Endo; Nobuyoshi Esaki; K Soda
Cloning and expression of the glutamate racemase gene of Bacillus pumilus.
Journal of biochemistry 1997;121(6):1155-61.
1996: K H Jhee; Tohru Yoshimura; Nobuyoshi Esaki; K Soda
Stereospecificity of thermostable ornithine 5-aminotransferase for the hydrogen transfer in the L- and D-ornithine transamination.
Biochemistry 1996;35(30):9792-6.
1996: Tohru Yoshimura; K H Jhee; K Soda
Stereospecificity for the hydrogen transfer and molecular evolution of pyridoxal enzymes.
Bioscience, biotechnology, and biochemistry 1996;60(2):181-7.
1995: K H Jhee; Tohru Yoshimura; Nobuyoshi Esaki; K Yonaha; K Soda
Thermostable ornithine aminotransferase from Bacillus sp. YM-2: purification and characterization.
Journal of biochemistry 1995;118(1):101-8.
1995: K Kishimoto; Tohru Yoshimura; Nobuyoshi Esaki; Shigetoshi Sugio; J M Manning; K Soda
Role of leucine 201 of thermostable D-amino acid aminotransferase from a thermophile, Bacillus sp. YM-1.
Journal of biochemistry 1995;117(4):691-6.
1995: M Ashiuchi; Tohru Yoshimura; T Kitamura; Yasushi Kawata; J Nagai; S Gorlatov; Nobuyoshi Esaki; K Soda
In vivo effect of GroESL on the folding of glutamate racemase of Escherichia coli.
Journal of biochemistry 1995;117(3):495-8.
1994: K Kataoka; K Tanizawa; Toshiaki Fukui; Hiroshi Ueno; Tohru Yoshimura; Nobuyoshi Esaki; K Soda
Identification of active site lysyl residues of phenylalanine dehydrogenase by chemical modification with methyl acetyl phosphate combined with site-directed mutagenesis.
Journal of biochemistry 1994;116(6):1370-6.
1994: S Y Choi; Nobuyoshi Esaki; M Ashiuchi; Tohru Yoshimura; K Soda
Bacterial glutamate racemase has high sequence similarity with myoglobins and forms an equimolar inactive complex with hemin.
Proceedings of the National Academy of Sciences of the United States of America 1994;91(21):10144-7.
1994: K Kataoka; H Takada; K Tanizawa; Tohru Yoshimura; Nobuyoshi Esaki; T Ohshima; K Soda
Construction and characterization of chimeric enzyme consisting of an amino-terminal domain of phenylalanine dehydrogenase and a carboxy-terminal domain of leucine dehydrogenase.
Journal of biochemistry 1994;116(4):931-6.
1994: D W Kim; Tohru Yoshimura; Nobuyoshi Esaki; E Satoh; K Soda
Studies of the active-site lysyl residue of thermostable aspartate aminotransferase: combination of site-directed mutagenesis and chemical modification.
Journal of biochemistry 1994;115(1):93-7.
1994: Y Matsushima; D W Kim; Tohru Yoshimura; Seiki Kuramitsu; Hiroyuki Kagamiyama; Nobuyoshi Esaki; K Soda
Replacement of active-site lysine-239 of thermostable aspartate aminotransferase by S-(2-aminoethyl)cysteine: properties of the mutant enzyme.
Journal of biochemistry 1994;115(1):108-12.
1993: Tohru Yoshimura; M Ashiuchi; Nobuyoshi Esaki; C Kobatake; S Y Choi; K Soda
Expression of glr (murI, dga) gene encoding glutamate racemase in Escherichia coli.
The Journal of biological chemistry 1993;268(32):24242-6.
1993: K Kataoka; H Takada; Tohru Yoshimura; S Furuyoshi; Nobuyoshi Esaki; T Ohshima; K Soda
Site-directed mutagenesis of a hexapeptide segment involved in substrate recognition of phenylalanine dehydrogenase from Thermoactinomyces intermedius.
Journal of biochemistry 1993;114(1):69-75.
1992: S Y Choi; Nobuyoshi Esaki; Tohru Yoshimura; K Soda
Reaction mechanism of glutamate racemase, a pyridoxal phosphate-independent amino acid racemase.
Journal of biochemistry 1992;112(1):139-42.
1991: H Toyama; Nobuyoshi Esaki; Tohru Yoshimura; K Tanizawa; K Soda
Thermostable alanine racemase of Bacillus stearothermophilus: subunit dissociation and unfolding.
Journal of biochemistry 1991;110(2):279-83.
1991: H Toyama; K Tanizawa; Tohru Yoshimura; S Asano; Y H Lim; Nobuyoshi Esaki; K Soda
Thermostable alanine racemase of Bacillus stearothermophilus. Construction and expression of active fragmentary enzyme.
The Journal of biological chemistry 1991;266(21):13634-9.
1991: K Nishimura; K Tanizawa; Tohru Yoshimura; Nobuyoshi Esaki; S Futaki; J M Manning; K Soda
Effect of substitution of a lysyl residue that binds pyridoxal phosphate in thermostable D-amino acid aminotransferase by arginine and alanine.
Biochemistry 1991;30(16):4072-7.
1991: H Takada; Tohru Yoshimura; T Ohshima; Nobuyoshi Esaki; K Soda
Thermostable phenylalanine dehydrogenase of Thermoactinomyces intermedius: cloning, expression, and sequencing of its gene.
Journal of biochemistry 1991;109(3):371-6.
1991: S Y Choi; Nobuyoshi Esaki; Tohru Yoshimura; K Soda
Overproduction of glutamate racemase of Pediococcus pentosaceus in Escherichia coli clone cells and its purification.
Protein expression and purification 1991;2(1):90-3.
1991: T Yagi; H Misono; K Tanizawa; Tohru Yoshimura; K Soda
Characterization of the half and overall reactions catalyzed by L-lysine:2-oxoglutarate 6-aminotransferase.
Journal of biochemistry 1991;109(1):61-5.
1990: Tohru Yoshimura; Y Matsushima; K Tanizawa; M H Sung; T Yamauchi; M Wakayama; Nobuyoshi Esaki; K Soda
Substitution of S-(beta-aminoethyl)-cysteine for active-site lysine of thermostable aspartate aminotransferase.
Journal of biochemistry 1990;108(5):699-700.