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Massimo Stefani

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Massimo Stefani
Structural features and cytotoxicity of amyloid oligomers: implications in Alzheimer's disease and other diseases with amyloid deposits.
Elisa Evangelisti; Caterina Sgromo; Matteo Becatti; Roberta Cascella; Cristina Cecchi; Fabrizio Chiti; Christopher M Dobson; Massimo Stefani
Membrane lipid composition and its physicochemical properties define cell vulnerability to aberrant protein oligomers.
Massimo Stefani
Biochemical and biophysical features of both oligomer/fibril and cell membrane in amyloid cytotoxicity.

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All Publications

2012: Massimo Stefani
Structural features and cytotoxicity of amyloid oligomers: implications in Alzheimer's disease and other diseases with amyloid deposits.
Progress in neurobiology 2012;99(3):226-45.
2012: Elisa Evangelisti; Caterina Sgromo; Matteo Becatti; Roberta Cascella; Cristina Cecchi; Fabrizio Chiti; Christopher M Dobson; Massimo Stefani
Membrane lipid composition and its physicochemical properties define cell vulnerability to aberrant protein oligomers.
Journal of cell science 2012;125(Pt 10):2416-27.
2010: Massimo Stefani
Biochemical and biophysical features of both oligomer/fibril and cell membrane in amyloid cytotoxicity.
The FEBS journal 2010;277(22):4602-13.
2010: Massimo Stefani
Structural polymorphism of amyloid oligomers and fibrils underlies different fibrillization pathways: immunogenicity and cytotoxicity.
Current protein & peptide science 2010;11(5):343-54.
2010: Stefania Rigacci; Valentina Guidotti; monica bucciantini; Matteo Parri; Chiara Nediani; Elisabetta Cerbai; Massimo Stefani; Andrea Berti
Oleuropein aglycon prevents cytotoxic amyloid aggregation of human amylin.
The Journal of nutritional biochemistry 2010;21(8):726-35.
2010: Silvia Campioni; Benedetta Mannini; Mariagioia Zampagni; Anna Pensalfini; Claudia Parrini; Elisa Evangelisti; Annalisa Relini; Massimo Stefani; Christopher M Dobson; Cristina Cecchi; Fabrizio Chiti
A causative link between the structure of aberrant protein oligomers and their toxicity.
Nature chemical biology 2010;6(2):140-7.
2010: Massimo Stefani
Protein aggregation diseases: toxicity of soluble prefibrillar aggregates and their clinical significance.
Methods in molecular biology (Clifton, N.J.) 2010;648():25-41.
2009: Cristina Cecchi; Daniela Nichino; Mariagioia Zampagni; Caterina Bernacchioni; Elisa Evangelisti; Anna Pensalfini; gianfranco liguri; Alessandra Gliozzi; Massimo Stefani; Annalisa Relini
A protective role for lipid raft cholesterol against amyloid-induced membrane damage in human neuroblastoma cells.
Biochimica et biophysica acta 2009;1788(10):2204-16.
2009: Francesca Magherini; Laura Pieri; Francesca Guidi; Chiara Giangrande; Angela Amoresano; monica bucciantini; Massimo Stefani; Alessandra Modesti
Proteomic analysis of cells exposed to prefibrillar aggregates of HypF-N.
Biochimica et biophysica acta 2009;1794(8):1243-50.
2009: Laura Pieri; monica bucciantini; Patrizio Guasti; Jimmy Savistchenko; Ronald Melki; Massimo Stefani
Synthetic lipid vesicles recruit native-like aggregates and affect the aggregation process of the prion Ure2p: insights on vesicle permeabilization and charge selectivity.
Biophysical journal 2009;96(8):3319-30.
2009: Sofia Giorgetti; Sara Raimondi; Silvia Cassinelli; monica bucciantini; Massimo Stefani; Gina Gregorini; Giulia Albonico; Remigio Moratti; Giovanni Montagna; Monica Stoppini; Vittorio Bellotti
beta2-Microglobulin is potentially neurotoxic, but the blood brain barrier is likely to protect the brain from its toxicity.
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association 2009;24(4):1176-81.
2009: Massimo Stefani; gianfranco liguri
Cholesterol in Alzheimer's disease: unresolved questions.
Current Alzheimer research 2009;6(1):15-29.
2008: Massimo Stefani
Protein folding and misfolding on surfaces.
International journal of molecular sciences 2008;9(12):2515-42.
2008: Cristina Cecchi; Anna Pensalfini; gianfranco liguri; Serena Baglioni; Claudia Fiorillo; Simone Guadagna; Mariagioia Zampagni; Lucia Formigli; Daniele Nosi; Massimo Stefani
Differentiation increases the resistance of neuronal cells to amyloid toxicity.
Neurochemical research 2008;33(12):2516-31.
2008: Francesca Pellistri; monica bucciantini; Annalisa Relini; Daniele Nosi; Alessandra Gliozzi; Mauro Robello; Massimo Stefani
Nonspecific interaction of prefibrillar amyloid aggregates with glutamatergic receptors results in Ca2+ increase in primary neuronal cells.
The Journal of biological chemistry 2008;283(44):29950-60.
2008: Cristina Cecchi; Fabiana Rosati; Anna Pensalfini; Lucia Formigli; Daniele Nosi; gianfranco liguri; F Dichiara; M Morello; Giovanna Danza; Giuseppe Pieraccini; Alessandro Peri; Mario Serio; Massimo Stefani
Seladin-1/DHCR24 protects neuroblastoma cells against Abeta toxicity by increasing membrane cholesterol content.
Journal of cellular and molecular medicine 2008;12(5B):1990-2002.
2008: Francesco Bemporad; Joerg Gsponer; Harri I Hopearuoho; Georgia Plakoutsi; Gianmarco Stati; Massimo Stefani; Niccolò Taddei; Michele Vendruscolo; Fabrizio Chiti
Biological function in a non-native partially folded state of a protein.
The EMBO journal 2008;27(10):1525-35.
2008: Stefania Rigacci; monica bucciantini; Annalisa Relini; Alessandra Pesce; Alessandra Gliozzi; Andrea Berti; Massimo Stefani
The (1-63) region of the p53 transactivation domain aggregates in vitro into cytotoxic amyloid assemblies.
Biophysical journal 2008;94(9):3635-46.
2008: Cristina Cecchi; Anna Pensalfini; Massimo Stefani; Silvana Baglioni; Claudia Fiorillo; Silvia Cappadona; Roberto Caporale; Daniele Nosi; Marco Ruggiero; gianfranco liguri
Replicating neuroblastoma cells in different cell cycle phases display different vulnerability to amyloid toxicity.
Journal of molecular medicine (Berlin, Germany) 2008;86(2):197-209.
2007: Massimo Stefani
Generic cell dysfunction in neurodegenerative disorders: role of surfaces in early protein misfolding, aggregation, and aggregate cytotoxicity.
The Neuroscientist : a review journal bringing neurobiology, neurology and psychiatry 2007;13(5):519-31.
2006: Matteo Ramazzotti; Claudia Parrini; Massimo Stefani; Giampaolo Manao; Donatella Degl'Innocenti
The intrachain disulfide bridge is responsible of the unusual stability properties of novel acylphosphatase from Escherichia coli.
FEBS letters 2006;580(30):6763-8.
2006: Claudio Canale; Silvia Torrassa; Pasquale Rispoli; Annalisa Relini; Ranieri Rolandi; monica bucciantini; Massimo Stefani; Alessandra Gliozzi
Natively folded HypF-N and its early amyloid aggregates interact with phospholipid monolayers and destabilize supported phospholipid bilayers.
Biophysical journal 2006;91(12):4575-88.
2006: Massimo Stefani
Biological surfaces as catalysts of amyloid aggregate nucleation and primary sites of amyloid toxicity.
The Italian journal of biochemistry 2006;55(3-4):194-204.
2006: Silvana Baglioni; Fiorella Casamenti; monica bucciantini; Leila M Luheshi; Niccolò Taddei; Fabrizio Chiti; Christopher M Dobson; Massimo Stefani
Prefibrillar amyloid aggregates could be generic toxins in higher organisms.
The Journal of neuroscience : the official journal of the Society for Neuroscience 2006;26(31):8160-7.
2006: Laura Pieri; monica bucciantini; Daniele Nosi; Lucia Formigli; Jimmy Savistchenko; Ronald Melki; Massimo Stefani
The yeast prion Ure2p native-like assemblies are toxic to mammalian cells regardless of their aggregation state.
The Journal of biological chemistry 2006;281(22):15337-44.
2006: Cristina Cecchi; Anna Pensalfini; Silvana Baglioni; Claudia Fiorillo; Roberto Caporale; Lucia Formigli; gianfranco liguri; Massimo Stefani
Differing molecular mechanisms appear to underlie early toxicity of prefibrillar HypF-N aggregates to different cell types.
The FEBS journal 2006;273(10):2206-22.
2006: Francesco Bemporad; Niccolò Taddei; Massimo Stefani; Fabrizio Chiti
Assessing the role of aromatic residues in the amyloid aggregation of human muscle acylphosphatase.
Protein science : a publication of the Protein Society 2006;15(4):862-70.
2006: Alessandra Corazza; Camillo Rosano; Katiuscia Pagano; Vera Alverdi; Gennaro Esposito; Cristina Capanni; Francesco Bemporad; Georgia Plakoutsi; Massimo Stefani; Fabrizio Chiti; Simone Zuccotti; Martino Bolognesi; Paolo Viglino
Structure, conformational stability, and enzymatic properties of acylphosphatase from the hyperthermophile Sulfolobus solfataricus.
Proteins 2006;62(1):64-79.
2005: Cristina Cecchi; Silvana Baglioni; Claudia Fiorillo; Anna Pensalfini; gianfranco liguri; Daniele Nosi; Stefania Rigacci; monica bucciantini; Massimo Stefani
Insights into the molecular basis of the differing susceptibility of varying cell types to the toxicity of amyloid aggregates.
Journal of cell science 2005;118(Pt 15):3459-70.
2005: Giulia Calloni; Sara Zoffoli; Massimo Stefani; Christopher M Dobson; Fabrizio Chiti
Investigating the effects of mutations on protein aggregation in the cell.
The Journal of biological chemistry 2005;280(11):10607-13.
2005: monica bucciantini; Stefania Rigacci; Andrea Berti; Laura Pieri; Cristina Cecchi; Daniele Nosi; Lucia Formigli; Fabrizio Chiti; Massimo Stefani
Patterns of cell death triggered in two different cell lines by HypF-N prefibrillar aggregates.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2005;19(3):437-9.
2005: Martino Calamai; Claudio Canale; Annalisa Relini; Massimo Stefani; Fabrizio Chiti; Christopher M Dobson
Reversal of protein aggregation provides evidence for multiple aggregated States.
Journal of molecular biology 2005;346(2):603-16.
2005: Simone Zuccotti; Camillo Rosano; Francesco Bemporad; Massimo Stefani; Martino Bolognesi
Preliminary characterization of two different crystal forms of acylphosphatase from the hyperthermophile archaeon Sulfolobus solfataricus.
Acta crystallographica. Section F, Structural biology and crystallization communications 2005;61(Pt 1):144-6.
2004: Massimo Stefani
Protein misfolding and aggregation: new examples in medicine and biology of the dark side of the protein world.
Biochimica et biophysica acta 2004;1739(1):5-25.
2004: monica bucciantini; Giulia Calloni; Fabrizio Chiti; Lucia Formigli; Daniele Nosi; Christopher M Dobson; Massimo Stefani
Prefibrillar amyloid protein aggregates share common features of cytotoxicity.
The Journal of biological chemistry 2004;279(30):31374-82.
2004: Francesco Bemporad; Cristina Capanni; Martino Calamai; Maria Luisa Tutino; Massimo Stefani; Fabrizio Chiti
Studying the folding process of the acylphosphatase from Sulfolobus solfataricus. A comparative analysis with other proteins from the same superfamily.
Biochemistry 2004;43(28):9116-26.
2004: Simone Zuccotti; Camillo Rosano; Matteo Ramazzotti; Donatella Degl'Innocenti; Massimo Stefani; Giampaolo Manao; Martino Bolognesi
Three-dimensional structural characterization of a novel Drosophila melanogaster acylphosphatase.
Acta crystallographica. Section D, Biological crystallography 2004;60(Pt 6):1177-9.
2004: Annalisa Relini; Silvia Torrassa; Ranieri Rolandi; Alessandra Gliozzi; Camillo Rosano; Claudio Canale; Martino Bolognesi; Georgia Plakoutsi; monica bucciantini; Fabrizio Chiti; Massimo Stefani
Monitoring the process of HypF fibrillization and liposome permeabilization by protofibrils.
Journal of molecular biology 2004;338(5):943-57.
2004: Georgia Plakoutsi; Niccolò Taddei; Massimo Stefani; Fabrizio Chiti
Aggregation of the Acylphosphatase from Sulfolobus solfataricus: the folded and partially unfolded states can both be precursors for amyloid formation.
The Journal of biological chemistry 2004;279(14):14111-9.
2004: Cristina Capanni; Niccolò Taddei; Silvia Gabrielli; Luigi Messori; Pierluigi Orioli; Fabrizio Chiti; Massimo Stefani; Giampietro Ramponi
Investigation of the effects of copper ions on protein aggregation using a model system.
Cellular and molecular life sciences : CMLS 2004;61(7-8):982-91.
2004: Donatella Degl'Innocenti; Niccolò Taddei; Matteo Ramazzotti; Massimo Stefani; Fabrizio Chiti; Giampietro Ramponi
Selection of antibody fragments specific for an alpha-helix region of acylphosphatase.
Journal of molecular recognition : JMR 2004;17(1):62-6.
2003: Martino Calamai; Niccolò Taddei; Massimo Stefani; Giampietro Ramponi; Fabrizio Chiti
Relative influence of hydrophobicity and net charge in the aggregation of two homologous proteins.
Biochemistry 2003;42(51):15078-83.
2003: Massimo Stefani
What the use of disease-unrelated model proteins can tell us about the molecular basis of amyloid aggregation and toxicity.
The Italian journal of biochemistry 2003;52(4):162-76.
2003: Massimo Stefani; Christopher M Dobson
Protein aggregation and aggregate toxicity: new insights into protein folding, misfolding diseases and biological evolution.
Journal of molecular medicine (Berlin, Germany) 2003;81(11):678-99.
2003: Fabrizio Chiti; Massimo Stefani; Niccolò Taddei; Giampietro Ramponi; Christopher M Dobson
Rationalization of the effects of mutations on peptide and protein aggregation rates.
Nature 2003;424(6950):805-8.
2003: Giulia Calloni; Niccolò Taddei; Kevin W Plaxco; Giampietro Ramponi; Massimo Stefani; Fabrizio Chiti
Comparison of the folding processes of distantly related proteins. Importance of hydrophobic content in folding.
Journal of molecular biology 2003;330(3):577-91.
2002: Fabrizio Chiti; Martino Calamai; Niccolò Taddei; Massimo Stefani; Giampietro Ramponi; Christopher M Dobson
Studies of the aggregation of mutant proteins in vitro provide insights into the genetics of amyloid diseases.
Proceedings of the National Academy of Sciences of the United States of America 2002;99 Suppl 4():16419-26.
2002: Camillo Rosano; Simone Zuccotti; monica bucciantini; Massimo Stefani; Giampietro Ramponi; Martino Bolognesi
Crystal structure and anion binding in the prokaryotic hydrogenase maturation factor HypF acylphosphatase-like domain.
Journal of molecular biology 2002;321(5):785-96.
2002: monica bucciantini; Elisa Giannoni; Fabrizio Chiti; Fabiana Baroni; Lucia Formigli; Jesús Zurdo; Niccolò Taddei; Giampietro Ramponi; Christopher M Dobson; Massimo Stefani
Inherent toxicity of aggregates implies a common mechanism for protein misfolding diseases.
Nature 2002;416(6880):507-11.
2002: Camillo Rosano; Simone Zuccotti; Massimo Stefani; monica bucciantini; Giampietro Ramponi; Martino Bolognesi
Crystallization and preliminary X-ray characterization of the acylphosphatase-like domain from the Escherichia coli hydrogenase maturation factor HypF.
Acta crystallographica. Section D, Biological crystallography 2002;58(Pt 3):524-5.
2002: Fabrizio Chiti; Niccolò Taddei; Fabiana Baroni; Cristina Capanni; Massimo Stefani; Giampietro Ramponi; Christopher M Dobson
Kinetic partitioning of protein folding and aggregation.
Nature structural biology 2002;9(2):137-43.
2001: Fabrizio Chiti; monica bucciantini; Cristina Capanni; Niccolò Taddei; Christopher M Dobson; Massimo Stefani
Solution conditions can promote formation of either amyloid protofilaments or mature fibrils from the HypF N-terminal domain.
Protein science : a publication of the Protein Society 2001;10(12):2541-7.
2001: Niccolò Taddei; Cristina Capanni; Fabrizio Chiti; Massimo Stefani; Christopher M Dobson; Giampietro Ramponi
Folding and aggregation are selectively influenced by the conformational preferences of the alpha-helices of muscle acylphosphatase.
The Journal of biological chemistry 2001;276(40):37149-54.
2001: Fabrizio Chiti; Niccolò Taddei; Massimo Stefani; Christopher M Dobson; Giampietro Ramponi
Reduction of the amyloidogenicity of a protein by specific binding of ligands to the native conformation.
Protein science : a publication of the Protein Society 2001;10(4):879-86.
2001: Fabrizio Chiti; Palma Mangione; Alessia Andreola; Sofia Giorgetti; Massimo Stefani; Christopher M Dobson; Vittorio Bellotti; Niccolò Taddei
Detection of two partially structured species in the folding process of the amyloidogenic protein beta 2-microglobulin.
Journal of molecular biology 2001;307(1):379-91.
2000: Thelma A Pertinhez; Daizo Hamada; Lorna J Smith; Fabrizio Chiti; Niccolò Taddei; Massimo Stefani; Christopher M Dobson
Initial denaturing conditions influence the slow folding phase of acylphosphatase associated with proline isomerization.
Protein science : a publication of the Protein Society 2000;9(8):1466-73.
2000: Niccolò Taddei; Fabrizio Chiti; Tania Fiaschi; monica bucciantini; Cristina Capanni; Massimo Stefani; L Serrano; Christopher M Dobson; Giampietro Ramponi
Stabilisation of alpha-helices by site-directed mutagenesis reveals the importance of secondary structure in the transition state for acylphosphatase folding.
Journal of molecular biology 2000;300(3):633-47.
1999: Fabrizio Chiti; Niccolò Taddei; P M White; monica bucciantini; Francesca Magherini; Massimo Stefani; Christopher M Dobson
Mutational analysis of acylphosphatase suggests the importance of topology and contact order in protein folding.
Nature structural biology 1999;6(11):1005-9.
1999: monica bucciantini; Paola Chiarugi; Paolo Cirri; Letizia Taddei; Massimo Stefani; Giovanni Raugei; Pär Nordlund; Giampietro Ramponi
The low Mr phosphotyrosine protein phosphatase behaves differently when phosphorylated at Tyr131 or Tyr132 by Src kinase.
FEBS letters 1999;456(1):73-8.
1999: Fabrizio Chiti; P Webster; Niccolò Taddei; Anne Clark; Massimo Stefani; Giampietro Ramponi; Christopher M Dobson
Designing conditions for in vitro formation of amyloid protofilaments and fibrils.
Proceedings of the National Academy of Sciences of the United States of America 1999;96(7):3590-4.
1999: Niccolò Taddei; Fabrizio Chiti; Paola Paoli; Tania Fiaschi; monica bucciantini; Massimo Stefani; Christopher M Dobson; Giampietro Ramponi
Thermodynamics and kinetics of folding of common-type acylphosphatase: comparison to the highly homologous muscle isoenzyme.
Biochemistry 1999;38(7):2135-42.
1998: Fabrizio Chiti; Niccolò Taddei; Nico A J van Nuland; Francesca Magherini; Massimo Stefani; Giampietro Ramponi; Christopher M Dobson
Structural characterization of the transition state for folding of muscle acylphosphatase.
Journal of molecular biology 1998;283(4):893-903.
1998: Fabrizio Chiti; Francesca Magherini; Niccolò Taddei; C Ilardi; Massimo Stefani; monica bucciantini; Christopher M Dobson; Giampietro Ramponi
Studies on enzymatic activity and conformational stability of muscle acylphosphatase mutated at conserved lysine residues.
Protein engineering 1998;11(7):557-61.
1998: Riccardo Marzocchini; monica bucciantini; Massimo Stefani; Niccolò Taddei; M G Thunnissen; Pär Nordlund; Giampietro Ramponi
Expression, purification and preliminary crystal analysis of the human low Mr phosphotyrosine protein phosphatase isoform 1.
FEBS letters 1998;426(1):52-6.
1998: Fabrizio Chiti; Nico A J van Nuland; Niccolò Taddei; Francesca Magherini; Massimo Stefani; Giampietro Ramponi; Christopher M Dobson
Conformational stability of muscle acylphosphatase: the role of temperature, denaturant concentration, and pH.
Biochemistry 1998;37(5):1447-55.
1998: monica bucciantini; Massimo Stefani; Niccolò Taddei; Fabrizio Chiti; Stefania Rigacci; Giampietro Ramponi
Sequence-specific recognition of peptide substrates by the low Mr phosphotyrosine protein phosphatase isoforms.
FEBS letters 1998;422(2):213-7.
1997: Giampietro Ramponi; Massimo Stefani
Structure and function of the low Mr phosphotyrosine protein phosphatases.
Biochimica et biophysica acta 1997;1341(2):137-56.
1997: Niccolò Taddei; Fabrizio Chiti; Francesca Magherini; Massimo Stefani; Marjolein M G M Thunnissen; Pär Nordlund; Giampietro Ramponi
Structural and kinetic investigations on the 15-21 and 42-45 loops of muscle acylphosphatase: evidence for their involvement in enzyme catalysis and conformational stabilization.
Biochemistry 1997;36(23):7217-24.
1997: Giampietro Ramponi; Massimo Stefani
Structural, catalytic, and functional properties of low M(r), phosphotyrosine protein phosphatases. Evidence of a long evolutionary history.
The international journal of biochemistry & cell biology 1997;29(2):279-92.
1997: Massimo Stefani; Niccolò Taddei; Giampietro Ramponi
Insights into acylphosphatase structure and catalytic mechanism.
Cellular and molecular life sciences : CMLS 1997;53(2):141-51.
1996: Niccolò Taddei; Massimo Stefani; Francesca Magherini; Fabrizio Chiti; Alessandra Modesti; Giovanni Raugei; Giampietro Ramponi
Looking for residues involved in the muscle acylphosphatase catalytic mechanism and structural stabilization: role of Asn41, Thr42, and Thr46.
Biochemistry 1996;35(22):7077-83.
1996: Niccolò Taddei; Francesca Magherini; Fabrizio Chiti; monica bucciantini; Giovanni Raugei; Massimo Stefani; Giampietro Ramponi
C-terminal region contributes to muscle acylphosphatase three-dimensional structure stabilisation.
FEBS letters 1996;384(2):172-6.
1996: Alessandra Modesti; Niccolò Taddei; Fabrizio Chiti; monica bucciantini; Francesca Magherini; Stefania Rigacci; Massimo Stefani; Giovanni Raugei; Giampietro Ramponi
Properties of Cys21-mutated muscle acylphosphatases.
Journal of protein chemistry 1996;15(1):27-34.
1995: Alessandra Modesti; Niccolò Taddei; monica bucciantini; Massimo Stefani; B Colombini; Giovanni Raugei; Giampietro Ramponi
Expression, purification, and characterization of acylphosphatase muscular isoenzyme as fusion protein with glutathione S-transferase.
Protein expression and purification 1995;6(6):799-805.
1995: Niccolò Taddei; Alessandra Modesti; monica bucciantini; Massimo Stefani; Francesca Magherini; M Vecchi; Giovanni Raugei; Giampietro Ramponi
Properties of N-terminus truncated and C-terminus mutated muscle acylphosphatases.
FEBS letters 1995;362(2):175-9.
1994: Niccolò Taddei; M Buck; R W Broadhurst; Massimo Stefani; Giampietro Ramponi; Christopher M Dobson
Equilibrium unfolding studies of horse muscle acylphosphatase.
European journal of biochemistry / FEBS 1994;225(3):811-7.
1994: Massimo Stefani; monica bucciantini; Niccolò Taddei; G Mincione; Giampaolo Manao; Giampietro Ramponi
Antisense peptides to the 43-57 region of acylphosphatase and to the 46-60 region of two isoenzymes of a low-M(r) phosphotyrosine protein phosphatase do not interact with the corresponding proteins.
Biotechnology and applied biochemistry 1994;20 ( Pt 2)():241-9.
1994: Niccolò Taddei; Massimo Stefani; M Vecchi; Alessandra Modesti; Giovanni Raugei; monica bucciantini; Francesca Magherini; Giampietro Ramponi
Arginine-23 is involved in the catalytic site of muscle acylphosphatase.
Biochimica et biophysica acta 1994;1208(1):75-80.
1994: Niccolò Taddei; Paola Chiarugi; Paolo Cirri; Tania Fiaschi; Massimo Stefani; Guido Camici; Giovanni Raugei; Giampietro Ramponi
Aspartic-129 is an essential residue in the catalytic mechanism of the low M(r) phosphotyrosine protein phosphatase.
FEBS letters 1994;350(2-3):328-32.
1994: X D Su; Niccolò Taddei; Massimo Stefani; Giampietro Ramponi; Pär Nordlund
The crystal structure of a low-molecular-weight phosphotyrosine protein phosphatase.
Nature 1994;370(6490):575-8.
1994: X D Su; E G Agango; Niccolò Taddei; monica bucciantini; Massimo Stefani; Giampietro Ramponi; Pär Nordlund
Crystallisation of a low molecular weight phosphotyrosine protein phosphatase from bovine liver.
FEBS letters 1994;343(2):107-8.
1993: Massimo Stefani; Anna Caselli; monica bucciantini; Luigia Pazzagli; F Dolfi; Guido Camici; Giampaolo Manao; Giampietro Ramponi
Dephosphorylation of tyrosine phosphorylated synthetic peptides by rat liver phosphotyrosine protein phosphatase isoenzymes.
FEBS letters 1993;326(1-3):131-4.
1992: Andrea Berti; Donatella Degl'Innocenti; Massimo Stefani; Giampietro Ramponi
Expression and turnover of acylphosphatase (muscular isoenzyme) in L6 myoblasts during myogenesis.
Archives of biochemistry and biophysics 1992;294(1):261-4.
1991: Massimo Stefani; Gianni Cappugi; Luigia Pazzagli; Guido Camici; Giampaolo Manao; Niccolò Taddei; M Buck; Giampietro Ramponi
Preparation and properties of des-Tyr98 and des-Arg97-Tyr98 acylphosphatase (muscular isoenzyme).
International journal of peptide and protein research 1991;38(3):278-84.
1990: Massimo Stefani; Donatella Degl'Innocenti; Andrea Berti; Gianni Cappugi; Giampaolo Manao; Guido Camici; Giampietro Ramponi
Purification and characterization of acylphosphatase erythrocyte isoenzyme from turkey muscle.
Journal of protein chemistry 1990;9(5):633-40.
1990: Donatella Degl'Innocenti; Andrea Berti; Massimo Stefani; gianfranco liguri; Giampietro Ramponi
Immunoaffinity purification and immunoassay determination of human erythrocyte acylphosphatase.
Biotechnology and applied biochemistry 1990;12(4):450-9.
1990: Donatella Degl'Innocenti; Andrea Berti; Massimo Stefani; Giampietro Ramponi
Generation of monoclonal antibodies to human acylphosphatase (muscular isoenzyme) and application in solid-phase immunoassay.
Biotechnology and applied biochemistry 1990;12(3):292-300.
1989: Vladimir Saudek; R J Williams; Massimo Stefani; Giampietro Ramponi
Mobility of secondary structure units of horse-muscle acylphosphatase. Relation to antigenicity.
European journal of biochemistry / FEBS 1989;185(1):99-103.
1989: Vladimir Saudek; J Boyd; R J Williams; Massimo Stefani; Giampietro Ramponi
The sequence-specific assignment of the 1H-NMR spectrum of an enzyme, horse-muscle acylphosphatase.
European journal of biochemistry / FEBS 1989;182(1):85-93.
1989: Vladimir Saudek; Mark R Wormald; R J Williams; J Boyd; Massimo Stefani; Giampietro Ramponi
Identification and description of beta-structure in horse muscle acylphosphatase by nuclear magnetic resonance spectroscopy.
Journal of molecular biology 1989;207(2):405-15.
1989: Guido Camici; Giampaolo Manao; Gianni Cappugi; A Modesti; Massimo Stefani; Giampietro Ramponi
The complete amino acid sequence of the low molecular weight cytosolic acid phosphatase.
The Journal of biological chemistry 1989;264(5):2560-7.
1988: Giampietro Ramponi; gianfranco liguri; Chiara Nediani; Massimo Stefani; Niccolò Taddei; Paolo Nassi
Acylphosphatase increases the rate of ethanol production from glucose in cell-free extracts of Saccharomyces cerevisiae.
Biotechnology and applied biochemistry 1988;10(5):408-13.
1988: Massimo Stefani; Andrea Berti; Guido Camici; Giampaolo Manao; Donatella Degl'Innocenti; G Prakash; Riccardo Marzocchini; Giampietro Ramponi
Horse brain acylphosphatase: purification and characterization.
FEBS letters 1988;236(1):209-16.
1988: Giampaolo Manao; Gianni Cappugi; Alessandra Modesti; Massimo Stefani; Riccardo Marzocchini; Donatella Degl'Innocenti; Guido Camici
Guinea pig acylphosphatase: the amino acid sequence.
Journal of protein chemistry 1988;7(4):417-26.
1988: Andrea Berti; Massimo Stefani; Donatella Degl'Innocenti; M Ruggiero; Vincenzo Chiarugi; G Ramponi
Effect of exogenously added acylphosphatases on inositol lipid metabolism in human platelets.
FEBS letters 1988;235(1-2):229-32.
1987: Andrea Berti; Donatella Degl'Innocenti; Massimo Stefani; gianfranco liguri; G Ramponi
Quantitative determination of acylphosphatase levels in horse tissues by enzyme-linked immunosorbent assay.
The Italian journal of biochemistry 1987;36(2):82-91.
1986: Andrea Berti; Massimo Stefani; Guido Camici; Giampaolo Manao; Gianni Cappugi; Donatella Degl'Innocenti; G Ramponi
Purification and characterization of rabbit muscle acylphosphatase in the thiol (-SH) form.
International journal of peptide and protein research 1986;28(1):15-21.
1986: Guido Camici; Giampaolo Manao; A Modesti; Massimo Stefani; Andrea Berti; Gianni Cappugi; G Ramponi
The complete amino acid sequence of bovine skeletal muscle acylphosphatase.
The Italian journal of biochemistry 1986;35(1):1-15.
1985: Paolo Nassi; gianfranco liguri; N Landi; Andrea Berti; Massimo Stefani; B Pavolini; G Ramponi
Acylphosphatase from human skeletal muscle: purification, some properties and levels in normal and myopathic muscles.
Biochemical medicine 1985;34(2):166-75.
1985: Giampaolo Manao; Guido Camici; Gianni Cappugi; Massimo Stefani; gianfranco liguri; Andrea Berti; G Ramponi
Rabbit skeletal muscle acylphosphatase: the amino acid sequence of form Ra1.
Archives of biochemistry and biophysics 1985;241(2):418-24.
1985: Massimo Stefani; Guido Camici; Giampaolo Manao; Gianni Cappugi; gianfranco liguri; Donatella Degl'Innocenti; N Landi; Andrea Berti
Horse heart acylphosphatase: purification and characterization.
The Italian journal of biochemistry 1985;34(2):94-108.
1984: Giampaolo Manao; Guido Camici; A Modesti; gianfranco liguri; Andrea Berti; Massimo Stefani; Gianni Cappugi; G Ramponi
Human skeletal muscle acylphosphatase: the primary structure.
Molecular biology & medicine 1984;2(6):369-78.
1984: gianfranco liguri; Paolo Nassi; Guido Camici; Giampaolo Manao; Gianni Cappugi; Massimo Stefani; Andrea Berti; G Ramponi
Studies on synthesis and degradation rates and some molecular properties of guinea-pig muscle acylphosphatase.
The Biochemical journal 1984;217(2):499-505.
1984: Guido Camici; Giampaolo Manao; Massimo Stefani; Andrea Berti; Gianni Cappugi; gianfranco liguri; G Ramponi
Turkey muscle acylphosphatase: purification and comparative studies.
The Italian journal of biochemistry 1984;33(1):1-16.
1983: Guido Camici; Giampaolo Manao; Gianni Cappugi; Andrea Berti; Massimo Stefani; gianfranco liguri; G Ramponi
The primary structure of turkey muscle acylphosphatase.
European journal of biochemistry / FEBS 1983;137(1-2):269-77.
1983: Giampaolo Manao; Guido Camici; Massimo Stefani; Andrea Berti; Gianni Cappugi; gianfranco liguri; Paolo Nassi; G Ramponi
Affinity chromatographic purification of horse muscle acylphosphatase: evidence of the existence of multiple molecular forms.
Archives of biochemistry and biophysics 1983;226(2):414-24.
1982: gianfranco liguri; Massimo Stefani; Andrea Berti; Paolo Nassi; G Ramponi
Ca2+-ATPase from sarcoplasmic reticulum: acylphosphatase activity.
Archives of biochemistry and biophysics 1982;217(1):120-30.
1982: Andrea Berti; gianfranco liguri; Massimo Stefani; Paolo Nassi; G Ramponi
Purification of horse muscle acylphosphatase antibodies by affinity chromatography.
Physiological chemistry and physics 1982;14(3):307-11.
1981: Massimo Stefani; gianfranco liguri; Andrea Berti; Paolo Nassi; G Ramponi
Hydrolysis by horse muscle acylphosphatase of (Ca2+ + Mg2+)-ATPase phosphorylated intermediate.
Archives of biochemistry and biophysics 1981;208(1):37-41.
1980: gianfranco liguri; Massimo Stefani; Andrea Berti; Paolo Nassi; G Ramponi
Effect of acylphosphates on Ca2+ uptake by sarcoplasmic reticulum vesicles.
Archives of biochemistry and biophysics 1980;200(2):357-63.
1979: G Ramponi; G Camici; G Cappugi; P Nassi; G Manao; A Berti; Massimo Stefani
Horse muscle acylphosphatase: a more rapid purification procedure and crystallization.
The Italian journal of biochemistry 1979;28(6):433-40.
1979: Massimo Stefani; A Berti; G Camici; G Manao; G Cappugi; G Ramponi
Preparation and some properties of a dimeric form (S-S) of horse muscle acylphosphatase.
International journal of peptide and protein research 1979;14(3):227-33.
1978: Massimo Stefani; A Berti; G Camici; G Manao; G Cappugi; G Ramponi
Stability and kinetic behavior of carboxymethylated horse muscle acylphosphatase.
Physiological chemistry and physics 1978;10(4):367-73.
1978: A Berti; Massimo Stefani; G Camici; G Manao; G Ramponi
Stability of horse muscle acylphosphatase to heat and to urea.
Physiological chemistry and physics 1978;10(2):153-62.