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Richard Burgess

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Amanda M Esch; Jennifer A Lamberski; Janet E Mertz; Nancy E Thompson; Richard R Burgess
Production and characterization of monoclonal antibodies to estrogen-related receptor alpha (ERRα) and use in immunoaffinity chromatography.
Richard R Burgess; James D Watson
Fusion tags: a collection of papers.
Robert S Chumanov; Richard R Burgess
Artifact-inducing enrichment of ethylenediaminetetraacetic acid and ethyleneglycoltetraacetic acid on anion exchange resins.

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All Publications

2012: Amanda M Esch; Jennifer A Lamberski; Janet E Mertz; Nancy E Thompson; Richard R Burgess
Production and characterization of monoclonal antibodies to estrogen-related receptor alpha (ERRα) and use in immunoaffinity chromatography.
Protein expression and purification 2012;84(1):47-58.
2012: Richard R Burgess; James D Watson
Fusion tags: a collection of papers.
Protein expression and purification 2012;81(1):A1-2.
2011: Robert S Chumanov; Richard R Burgess
Artifact-inducing enrichment of ethylenediaminetetraacetic acid and ethyleneglycoltetraacetic acid on anion exchange resins.
Analytical biochemistry 2011;412(1):34-9.
2011: Peter Kuhn; Rob Chumanov; Yidan Wang; Ying Ge; Richard R Burgess; Wei Xu
Automethylation of CARM1 allows coupling of transcription and mRNA splicing.
Nucleic acids research 2011;39(7):2717-26.
2011: Robert S Chumanov; Peter A Kuhn; Wei Xu; Richard R Burgess
Expression and purification of full-length mouse CARM1 from transiently transfected HEK293T cells using HaloTag technology.
Protein expression and purification 2011;76(2):145-53.
2010: Robert S Chumanov; Richard R Burgess
Expression, purification, and refolding of active Nrf2 transcription factor fused to protein transduction TAT tag.
Protein expression and purification 2010;74(2):280-8.
2010: Catherine E Vrentas; Tamas Gaal; Richard R Burgess; Richard L Gourse
An improved procedure for the purification of the Escherichia coli RNA polymerase omega subunit.
Protein expression and purification 2010;71(2):190-4.
2010: Kai Zhao; Mingzhu Liu; Richard R Burgess
Promoter and regulon analysis of nitrogen assimilation factor, sigma54, reveal alternative strategy for E. coli MG1655 flagellar biosynthesis.
Nucleic acids research 2010;38(4):1273-83.
2010: Xiaoping Sun; Elizabeth A Barlow; Shidong Ma; Stacy R Hagemeier; Sarah J Duellman; Richard R Burgess; Ross Tellam; Rajiv Khanna; Shannon C Kenney
Hsp90 inhibitors block outgrowth of EBV-infected malignant cells in vitro and in vivo through an EBNA1-dependent mechanism.
Proceedings of the National Academy of Sciences of the United States of America 2010;107(7):3146-51.
2009: Nancy E Thompson; Bryan T Glaser; Katherine M Foley; Zachary F Burton; Richard R Burgess
Minimal promoter systems reveal the importance of conserved residues in the B-finger of human transcription factor IIB.
The Journal of biological chemistry 2009;284(37):24754-66.
2009: Sarah J Duellman; Katie L Thompson; Joshua J Coon; Richard R Burgess
Phosphorylation sites of Epstein-Barr virus EBNA1 regulate its function.
The Journal of general virology 2009;90(Pt 9):2251-9.
2009: Sarah J Duellman; Richard R Burgess
Large-scale Epstein-Barr virus EBNA1 protein purification.
Protein expression and purification 2009;63(2):128-33.
2009: Richard R Burgess
Important but little known (or forgotten) artifacts in protein biochemistry.
Methods in enzymology 2009;463():813-20.
2009: Richard R Burgess
Elution of proteins from gels.
Methods in enzymology 2009;463():565-72.
2009: Nancy E Thompson; Katherine M Foley; Elizabeth S Stalder; Richard R Burgess
Identification, production, and use of polyol-responsive monoclonal antibodies for immunoaffinity chromatography.
Methods in enzymology 2009;463():475-94.
2009: Richard R Burgess
Protein precipitation techniques.
Methods in enzymology 2009;463():331-42.
2009: Richard R Burgess
Refolding solubilized inclusion body proteins.
Methods in enzymology 2009;463():259-82.
2009: Richard R Burgess
Preparing a purification summary table.
Methods in enzymology 2009;463():29-34.
2009: Richard R Burgess
Use of bioinformatics in planning a protein purification.
Methods in enzymology 2009;463():21-8.
2009: Bryan T Glaser; Veit Bergendahl; Larry C Anthony; Brian Olson; Richard R Burgess
Studying the salt dependence of the binding of sigma70 and sigma32 to core RNA polymerase using luminescence resonance energy transfer.
PloS one 2009;4(8):e6490.
2009: Sarah J Duellman; Richard R Burgess
Antigen-binding properties of monoclonal antibodies reactive with EBNA1 and use in immunoaffinity chromatography.
PloS one 2009;4(2):e4614.
2007: Bryan T Glaser; Veit Bergendahl; Nancy E Thompson; Brian Olson; Richard R Burgess
LRET-based HTS of a small-compound library for inhibitors of bacterial RNA polymerase.
Assay and drug development technologies 2007;5(6):759-68.
2007: Mitchell D Probasco; Nancy E Thompson; Richard R Burgess
Immunoaffinity purification and characterization of RNA polymerase from Shewanella oneidensis.
Protein expression and purification 2007;55(1):23-30.
2006: Zakee L Sabree; Veit Bergendahl; Mark R Liles; Richard R Burgess; Robert M Goodman; Jo Handelsman
Identification and characterization of the gene encoding the Acidobacterium capsulatum major sigma factor.
Gene 2006;376(1):144-51.
2006: Sarah J Duellman; Richard R Burgess
Overproduction in Escherichia coli and purification of Epstein-Barr virus EBNA-1.
Protein expression and purification 2006;47(2):434-40.
2006: Jennifer A Lamberski; Nancy E Thompson; Richard R Burgess
Expression and purification of a single-chain variable fragment antibody derived from a polyol-responsive monoclonal antibody.
Protein expression and purification 2006;47(1):82-92.
2006: Nancy E Thompson; Debra Bridges Jensen; Jennifer A Lamberski; Richard R Burgess
Purification of protein complexes by immunoaffinity chromatography: application to transcription machinery.
Genetic engineering 2006;27():81-100.
2005: Marni Raffaelle; Elenita I Kanin; Jennifer Vogt; Richard R Burgess; Aseem Z Ansari
Holoenzyme switching and stochastic release of sigma factors from RNA polymerase in vivo.
Molecular cell 2005;20(3):357-66.
2004: Nancy E Thompson; Katherine M Foley; Richard R Burgess
Antigen-binding properties of monoclonal antibodies reactive with human TATA-binding protein and use in immunoaffinity chromatography.
Protein expression and purification 2004;36(2):186-97.
2004: Dillon Phan; Chien-Jui Cheng; Matthew Galfione; Funda Vakar-Lopez; James Tunstead; Nancy E Thompson; Richard R Burgess; Sonia M Najjar; Li-Yuan Yu-Lee; Sue-Hwa Lin
Identification of Sp2 as a transcriptional repressor of carcinoembryonic antigen-related cell adhesion molecule 1 in tumorigenesis.
Cancer research 2004;64(9):3072-8.
2004: Sarah J Duellman; Nancy E Thompson; Richard R Burgess
An epitope tag derived from human transcription factor IIB that reacts with a polyol-responsive monoclonal antibody.
Protein expression and purification 2004;35(1):147-55.
2003: Nancy E Thompson; Terrance M Arthur; Richard R Burgess
Development of an epitope tag for the gentle purification of proteins by immunoaffinity chromatography: application to epitope-tagged green fluorescent protein.
Analytical biochemistry 2003;323(2):171-9.
2003: Veit Bergendahl; Nancy E Thompson; Katherine M Foley; Brian M Olson; Richard R Burgess
A cross-reactive polyol-responsive monoclonal antibody useful for isolation of core RNA polymerase from many bacterial species.
Protein expression and purification 2003;31(1):155-60.
2003: Veit Bergendahl; Bryan T Glaser; Richard R Burgess
A fast Western blot procedure improved for quantitative analysis by direct fluorescence labeling of primary antibodies.
Journal of immunological methods 2003;277(1-2):117-25.
2003: Veit Bergendahl; Tomasz Heyduk; Richard R Burgess
Luminescence resonance energy transfer-based high-throughput screening assay for inhibitors of essential protein-protein interactions in bacterial RNA polymerase.
Applied and environmental microbiology 2003;69(3):1492-8.
2003: Veit Bergendahl; Richard R Burgess
Studying sigma-core interactions in Escherichia coli RNA polymerase by electrophoretic shift assays and luminescence resonance energy transfer.
Methods in enzymology 2003;370():192-205.
2003: Larry C Anthony; Katherine M Foley; Nancy E Thompson; Richard R Burgess
Expression, purification of, and monoclonal antibodies to sigma factors from Escherichia coli.
Methods in enzymology 2003;370():181-92.
2002: Larry C Anthony; Richard R Burgess
Conformational flexibility in sigma70 region 2 during transcription initiation.
The Journal of biological chemistry 2002;277(48):46433-41.
2002: Veit Bergendahl; Larry C Anthony; Tomasz Heyduk; Richard R Burgess
On-column tris(2-carboxyethyl)phosphine reduction and IC5-maleimide labeling during purification of a RpoC fragment on a nickel-nitrilotriacetic acid Column.
Analytical biochemistry 2002;307(2):368-74.
2002: Richard R Burgess; Nancy E Thompson
Advances in gentle immunoaffinity chromatography.
Current opinion in biotechnology 2002;13(4):304-8.
2002: Larry C Anthony; Alan Anthony Dombkowski; Richard R Burgess
Using disulfide bond engineering to study conformational changes in the beta'260-309 coiled-coil region of Escherichia coli RNA polymerase during sigma(70) binding.
Journal of bacteriology 2002;184(10):2634-41.
2001: Tamas Gaal; Wilma Ross; ST Estrem; L H Nguyen; Richard R Burgess; Richard L Gourse
Promoter recognition and discrimination by EsigmaS RNA polymerase.
Molecular microbiology 2001;42(4):939-54.
2001: Tanja M Gruber; D Markov; Meghan M. Sharp; Brian A Young; Chi Zen Lu; Hong Ji Zhong; Irina Artsimovitch; Kati Geszvain; Terrance M Arthur; Richard R Burgess; Robert Landick; K Severinov; Carol A Gross
Binding of the initiation factor sigma(70) to core RNA polymerase is a multistep process.
Molecular cell 2001;8(1):21-31.
2001: Brian A Young; Larry C Anthony; Tanja M Gruber; Terrance M Arthur; Ewa Heyduk; Chi Zen Lu; Meghan M. Sharp; Tomasz Heyduk; Richard R Burgess; Carol A Gross
A coiled-coil from the RNA polymerase beta' subunit allosterically induces selective nontemplate strand binding by sigma(70).
Cell 2001;105(7):935-44.
2001: Nancy E Thompson; Richard R Burgess
Identification of polyol-responsive monoclonal antibodies for use in immunoaffinity chromatography.
Current protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.] 2001;Chapter 11():Unit11.18.
2001: Richard R Burgess; Larry C Anthony
How sigma docks to RNA polymerase and what sigma does.
Current opinion in microbiology 2001;4(2):126-31.
2000: C I Wooddell; Richard R Burgess
Topology of yeast RNA polymerase II subunits in transcription elongation complexes studied by photoaffinity cross-linking.
Biochemistry 2000;39(44):13405-21.
2000: Irina Artsimovitch; Vladimir Svetlov; Larry C Anthony; Richard R Burgess; Robert Landick
RNA polymerases from Bacillus subtilis and Escherichia coli differ in recognition of regulatory signals in vitro.
Journal of bacteriology 2000;182(21):6027-35.
2000: Larry C Anthony; Irina Artsimovitch; Vladimir Svetlov; Robert Landick; Richard R Burgess
Rapid purification of His(6)-tagged Bacillus subtilis core RNA polymerase.
Protein expression and purification 2000;19(3):350-4.
2000: Terrance M Arthur; Larry C Anthony; Richard R Burgess
Mutational analysis of beta '260-309, a sigma 70 binding site located on Escherichia coli core RNA polymerase.
The Journal of biological chemistry 2000;275(30):23113-9.
2000: Patrick Cramer; David A Bushnell; J Fu; Averell Gnatt; B Maier-Davis; Nancy E Thompson; Richard R Burgess; A M Edwards; PR David; Roger D Kornberg
Architecture of RNA polymerase II and implications for the transcription mechanism.
Science (New York, N.Y.) 2000;288(5466):640-9.
2000: Richard R Burgess; Terrance M Arthur; B C Pietz
Mapping protein-protein interaction domains using ordered fragment ladder far-western analysis of hexahistidine-tagged fusion proteins.
Methods in enzymology 2000;328():141-57.
1999: Nancy E Thompson; Richard R Burgess
Immunoaffinity purification of the RAP30 subunit of human transcription factor IIF.
Protein expression and purification 1999;17(2):260-6.
1999: S A McMahan; Richard R Burgess
Mapping protease susceptibility sites on the Escherichia coli transcription factor sigma70.
Biochemistry 1999;38(38):12424-31.
1999: J Fu; Averell Gnatt; David A Bushnell; Grant J Jensen; Nancy E Thompson; Richard R Burgess; PR David; Roger D Kornberg
Yeast RNA polymerase II at 5 A resolution.
Cell 1999;98(6):799-810.
1998: Terrance M Arthur; Richard R Burgess
Localization of a sigma70 binding site on the N terminus of the Escherichia coli RNA polymerase beta' subunit.
The Journal of biological chemistry 1998;273(47):31381-7.
1998: Vladimir Svetlov; K Nolan; Richard R Burgess
Rpb3, stoichiometry and sequence determinants of the assembly into yeast RNA polymerase II in vivo.
The Journal of biological chemistry 1998;273(18):10827-30.
1998: Richard R Burgess; Terrance M Arthur; B C Pietz
Interaction of Escherichia coli sigma 70 with core RNA polymerase.
Cold Spring Harbor symposia on quantitative biology 1998;63():277-87.
1997: L H Nguyen; Richard R Burgess
Comparative analysis of the interactions of Escherichia coli sigma S and sigma 70 RNA polymerase holoenzyme with the stationary-phase-specific bolAp1 promoter.
Biochemistry 1997;36(7):1748-54.
1997: M J Breyer; Nancy E Thompson; Richard R Burgess
Identification of the epitope for a highly cross-reactive monoclonal antibody on the major sigma factor of bacterial RNA polymerase.
Journal of bacteriology 1997;179(4):1404-8.
1996: L Rao; D P Jones; L H Nguyen; S A McMahan; Richard R Burgess
Epitope mapping using histidine-tagged protein fragments: application to Escherichia coli RNA polymerase sigma 70.
Analytical biochemistry 1996;241(2):173-9.
1996: L H Nguyen; Richard R Burgess
Overproduction and purification of sigmaS, the Escherichia coli stationary phase specific sigma transcription factor.
Protein expression and purification 1996;8(1):17-22.
1996: Paolo Landini; L I Hajec; L H Nguyen; Richard R Burgess; Michael R Volkert
The leucine-responsive regulatory protein (Lrp) acts as a specific repressor for sigma s-dependent transcription of the Escherichia coli aidB gene.
Molecular microbiology 1996;20(5):947-55.
1996: S A McMahan; Richard R Burgess
Single-step synthesis and characterization of biotinylated nitrilotriacetic acid, a unique reagent for the detection of histidine-tagged proteins immobilized on nitrocellulose.
Analytical biochemistry 1996;236(1):101-6.
1996: Nancy E Thompson; Richard R Burgess
Immunoaffinity purification of RNA polymerase II and transcription factors using polyol-responsive monoclonal antibodies.
Methods in enzymology 1996;274():513-26.
1996: Richard R Burgess
Purification of overproduced Escherichia coli RNA polymerase sigma factors by solubilizing inclusion bodies and refolding from Sarkosyl.
Methods in enzymology 1996;273():145-9.
1995: Nancy E Thompson; L A Strasheim; K M Nolan; Richard R Burgess
Accessibility of epitopes on human transcription factor IIB in the native protein and in a complex with DNA.
The Journal of biological chemistry 1995;270(9):4735-40.
1994: S A McMahan; Richard R Burgess
Use of aryl azide cross-linkers to investigate protein-protein interactions: an optimization of important conditions as applied to Escherichia coli RNA polymerase and localization of a sigma 70-alpha cross-link to the C-terminal region of alpha.
Biochemistry 1994;33(40):12092-9.
1994: Nancy E Thompson; Richard R Burgess
Purification of recombinant human transcription factor IIB by immunoaffinity chromatography.
Protein expression and purification 1994;5(5):468-75.
1993: D R Gentry; Richard R Burgess
Cross-linking of Escherichia coli RNA polymerase subunits: identification of beta' as the binding site of omega.
Biochemistry 1993;32(41):11224-7.
1993: L H Nguyen; D B Jensen; Nancy E Thompson; D R Gentry; Richard R Burgess
In vitro functional characterization of overproduced Escherichia coli katF/rpoS gene product.
Biochemistry 1993;32(41):11112-7.
1993: L H Nguyen; D B Jensen; Richard R Burgess
Overproduction and purification of sigma 32, the Escherichia coli heat shock transcription factor.
Protein expression and purification 1993;4(5):425-33.
1992: Nancy E Thompson; D A Hager; Richard R Burgess
Isolation and characterization of a polyol-responsive monoclonal antibody useful for gentle purification of Escherichia coli RNA polymerase.
Biochemistry 1992;31(30):7003-8.
1992: A B Buermeyer; Nancy E Thompson; L A Strasheim; Richard R Burgess; Peggy J Farnham
The HIP1 initiator element plays a role in determining the in vitro requirement of the dihydrofolate reductase gene promoter for the C-terminal domain of RNA polymerase II.
Molecular and cellular biology 1992;12(5):2250-9.
1992: D J Jin; Richard R Burgess; J P Richardson; Carol A Gross
Termination efficiency at rho-dependent terminators depends on kinetic coupling between RNA polymerase and rho.
Proceedings of the National Academy of Sciences of the United States of America 1992;89(4):1453-7.
1991: S A Lesley; M A Brow; Richard R Burgess
Use of in vitro protein synthesis from polymerase chain reaction-generated templates to study interaction of Escherichia coli transcription factors with core RNA polymerase and for epitope mapping of monoclonal antibodies.
The Journal of biological chemistry 1991;266(4):2632-8.
1990: S A Lesley; S B Jovanovich; Y C Tse-Dinh; Richard R Burgess
Identification of a heat shock promoter in the topA gene of Escherichia coli.
Journal of bacteriology 1990;172(12):6871-4.
1990: S I Gunderson; M W Knuth; Richard R Burgess
The human U1 snRNA promoter correctly initiates transcription in vitro and is activated by PSE1.
Genes & development 1990;4(12A):2048-60.
1990: M W Knuth; S I Gunderson; Nancy E Thompson; L A Strasheim; Richard R Burgess
Purification and characterization of proximal sequence element-binding protein 1, a transcription activating protein related to Ku and TREF that binds the proximal sequence element of the human U1 promoter.
The Journal of biological chemistry 1990;265(29):17911-20.
1990: D R Gentry; Richard R Burgess
Overproduction and purification of the omega subunit of Escherichia coli RNA polymerase.
Protein expression and purification 1990;1(1):81-6.
1990: D A Hager; D J Jin; Richard R Burgess
Use of Mono Q high-resolution ion-exchange chromatography to obtain highly pure and active Escherichia coli RNA polymerase.
Biochemistry 1990;29(34):7890-4.
1990: P H Blum; S B Jovanovich; M P McCann; J E Schultz; S A Lesley; Richard R Burgess; A Matin
Cloning and in vivo and in vitro regulation of cyclic AMP-dependent carbon starvation genes from Escherichia coli.
Journal of bacteriology 1990;172(7):3813-20.
1990: Nancy E Thompson; D B Aronson; Richard R Burgess
Purification of eukaryotic RNA polymerase II by immunoaffinity chromatography. Elution of active enzyme with protein stabilizing agents from a polyol-responsive monoclonal antibody.
The Journal of biological chemistry 1990;265(12):7069-77.
1990: T H Steinberg; D E Mathews; R D Durbin; Richard R Burgess
Tagetitoxin: a new inhibitor of eukaryotic transcription by RNA polymerase III.
The Journal of biological chemistry 1990;265(1):499-505.
1989: S A Lesley; Richard R Burgess
Characterization of the Escherichia coli transcription factor sigma 70: localization of a region involved in the interaction with core RNA polymerase.
Biochemistry 1989;28(19):7728-34.
1989: Nancy E Thompson; T H Steinberg; D B Aronson; Richard R Burgess
Inhibition of in vivo and in vitro transcription by monoclonal antibodies prepared against wheat germ RNA polymerase II that react with the heptapeptide repeat of eukaryotic RNA polymerase II.
The Journal of biological chemistry 1989;264(19):11511-20.
1989: S B Jovanovich; M T Record; Richard R Burgess
In an Escherichia coli coupled transcription-translation system, expression of the osmoregulated gene proU is stimulated at elevated potassium concentrations and by an extract from cells grown at high osmolality.
The Journal of biological chemistry 1989;264(14):7821-5.
1989: S B Jovanovich; S A Lesley; Richard R Burgess
In vitro use of monoclonal antibodies in Escherichia coli S-30 extracts to determine the RNA polymerase sigma subunit required by a promoter.
The Journal of biological chemistry 1989;264(7):3794-8.
1989: D R Gentry; Richard R Burgess
rpoZ, encoding the omega subunit of Escherichia coli RNA polymerase, is in the same operon as spoT.
Journal of bacteriology 1989;171(3):1271-7.
1988: S I Gunderson; J T Murphy; M W Knuth; T H Steinberg; J H Dahlberg; Richard R Burgess
Binding of transcription factors to the promoter of the human U1 RNA gene studied by footprinting.
The Journal of biological chemistry 1988;263(33):17603-10.
1988: M S Strickland; Nancy E Thompson; Richard R Burgess
Structure and function of the sigma-70 subunit of Escherichia coli RNA polymerase. Monoclonal antibodies: localization of epitopes by peptide mapping and effects on transcription.
Biochemistry 1988;27(15):5755-62.
1988: K A Chapman; S I Gunderson; M Anello; R D Wells; Richard R Burgess
Bacteriophage T7 late promoters with point mutations: quantitative footprinting and in vivo expression.
Nucleic acids research 1988;16(10):4511-24.
1988: S B Jovanovich; M Martinell; M T Record; Richard R Burgess
Rapid response to osmotic upshift by osmoregulated genes in Escherichia coli and Salmonella typhimurium.
Journal of bacteriology 1988;170(2):534-9.
1987: K A Chapman; Richard R Burgess
Construction of bacteriophage T7 late promoters with point mutations and characterization by in vitro transcription properties.
Nucleic acids research 1987;15(13):5413-32.
1987: S A Lesley; N E Thompson; Richard R Burgess
Studies of the role of the Escherichia coli heat shock regulatory protein sigma 32 by the use of monoclonal antibodies.
The Journal of biological chemistry 1987;262(11):5404-7.
1987: S I Gunderson; K A Chapman; Richard R Burgess
Interactions of T7 RNA polymerase with T7 late promoters measured by footprinting with methidiumpropyl-EDTA-iron(II).
Biochemistry 1987;26(6):1539-46.
1987: J T Murphy; J T Skuzeski; E Lund; T H Steinberg; Richard R Burgess; J E Dahlberg
Functional elements of the human U1 RNA promoter. Identification of five separate regions required for efficient transcription and template competition.
The Journal of biological chemistry 1987;262(4):1795-803.
1984: J M Skuzeski; E Lund; J T Murphy; T H Steinberg; Richard R Burgess; J E Dahlberg
Synthesis of human U1 RNA. II. Identification of two regions of the promoter essential for transcription initiation at position +1.
The Journal of biological chemistry 1984;259(13):8345-52.
1982: R S Boston; T J Miller; Janet E Mertz; Richard R Burgess
In Vitro Synthesis and Processing of Wheat alpha-Amylase : TRANSLATION OF GIBBERELLIC ACID-INDUCED WHEAT ALEURONE LAYER RNA BY WHEAT GERM AND XENOPUS LAEVIS OOCYTE SYSTEMS.
Plant physiology 1982;69(1):150-4.