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Alberto Di Donato

Research Profile

Chemicals & Drugs

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Genes & Molecular Sequences

Amino Acid Sequences

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Publications

TOP 3
Laura Bertini; Valeria Cafaro; Paola Capasso; Carlo Caporale; Carla Caruso; Silvia Proietti; Alberto Di Donato
Deepening TOL and TOU catabolic pathways of Pseudomonas sp. OX1: cloning, sequencing and characterization of the lower pathways.
Eugenio Notomista; Alessandro Pezzella; Roberta Scognamiglio; Luca Troncone; Alberto Di Donato; Giuliana Donadio; Viviana Izzo
Tuning the specificity of the recombinant multicomponent toluene o-xylene monooxygenase from Pseudomonas sp. strain OX1 for the biosynthesis of tyrosol from 2-phenylethanol.
Eugenio Notomista; Francesca Pennacchio; Valeria Cafaro; Giovanni Smaldone; Viviana Izzo; Luca Troncone; Mario Varcamonti; Alberto Di Donato
The marine isolate Novosphingobium sp. PP1Y shows specific adaptation to use the aromatic fraction of fuels as the sole carbon and energy source.

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All Publications

2013: Laura Bertini; Valeria Cafaro; Paola Capasso; Carlo Caporale; Carla Caruso; Silvia Proietti; Alberto Di Donato
Deepening TOL and TOU catabolic pathways of Pseudomonas sp. OX1: cloning, sequencing and characterization of the lower pathways.
Biochimie 2013;95(2):241-50.
2011: Eugenio Notomista; Alessandro Pezzella; Roberta Scognamiglio; Luca Troncone; Alberto Di Donato; Giuliana Donadio; Viviana Izzo
Tuning the specificity of the recombinant multicomponent toluene o-xylene monooxygenase from Pseudomonas sp. strain OX1 for the biosynthesis of tyrosol from 2-phenylethanol.
Applied and environmental microbiology 2011;77(15):5428-37.
2011: Eugenio Notomista; Francesca Pennacchio; Valeria Cafaro; Giovanni Smaldone; Viviana Izzo; Luca Troncone; Mario Varcamonti; Alberto Di Donato
The marine isolate Novosphingobium sp. PP1Y shows specific adaptation to use the aromatic fraction of fuels as the sole carbon and energy source.
Microbial ecology 2011;61(3):582-94.
2011: Viviana Izzo; Gabriella Leo; Roberta Scognamiglio; Luca Troncone; Leila Birolo; Alberto Di Donato
PHK from phenol hydroxylase of Pseudomonas sp. OX1. Insight into the role of an accessory protein in bacterial multicomponent monooxygenases.
Archives of biochemistry and biophysics 2011;505(1):48-59.
2010: Giuseppe Olivieri; Maria Elena Russo; Alberto Di Donato; Antonio Marzocchella; Piero Salatino
Unstable steady state operations of substrate inhibited cultures by dissolved oxygen control.
Journal of biotechnology 2010;156(4):302-8.
2009: Eugenio Notomista; Valeria Cafaro; Giuseppe Bozza; Alberto Di Donato
Molecular determinants of the regioselectivity of toluene/o-xylene monooxygenase from Pseudomonas sp. strain OX1.
Applied and environmental microbiology 2009;75(3):823-36.
2008: Serena Leone; Rosa Lanzetta; Roberta Scognamiglio; Fabiana Alfieri; Viviana Izzo; Alberto Di Donato; Michelangelo Parrilli; Otto Holst; Antonio Molinaro
The structure of the O-specific polysaccharide from the lipopolysaccharide of Pseudomonas sp. OX1 cultivated in the presence of the azo dye Orange II.
Carbohydrate research 2008;343(4):674-84.
2006: Loredana Siani; Rosanna Papa; Alberto Di Donato; Giovanni Sannia
Recombinant expression of Toluene o-Xylene Monooxygenase (ToMO) from Pseudomonas stutzeri OX1 in the marine Antarctic bacterium Pseudoalteromonas haloplanktis TAC125.
Journal of biotechnology 2006;126(3):334-41.
2006: Serena Leone; Antonio Molinaro; Fabiana Alfieri; Valeria Cafaro; Rosa Lanzetta; Alberto Di Donato; Michelangelo Parrilli
The biofilm matrix of Pseudomonas sp. OX1 grown on phenol is mainly constituted by alginate oligosaccharides.
Carbohydrate research 2006;341(14):2456-61.
2006: Eugenio Notomista; José M Mancheño; Orlando Crescenzi; Alberto Di Donato; José G Gavilanes; Giuseppe D'Alessio
The role of electrostatic interactions in the antitumor activity of dimeric RNases.
The FEBS journal 2006;273(16):3687-97.
2006: Loredana Siani; Ambra Viggiani; Eugenio Notomista; Alessandro Pezzella; Alberto Di Donato
The role of residue Thr249 in modulating the catalytic efficiency and substrate specificity of catechol-2,3-dioxygenase from Pseudomonas stutzeri OX1.
The FEBS journal 2006;273(13):2963-76.
2006: Ambra Viggiani; G Olivieri; Loredana Siani; Alberto Di Donato; A Marzocchella; P Salatino; Paola Barbieri; Enrica Galli
An airlift biofilm reactor for the biodegradation of phenol by Pseudomonas stutzeri OX1.
Journal of biotechnology 2006;123(4):464-77.
2005: Valeria Cafaro; Eugenio Notomista; Paola Capasso; Alberto Di Donato
Mutation of glutamic acid 103 of toluene o-xylene monooxygenase as a means to control the catabolic efficiency of a recombinant upper pathway for degradation of methylated aromatic compounds.
Applied and environmental microbiology 2005;71(8):4744-50.
2005: Valeria Cafaro; Eugenio Notomista; Paola Capasso; Alberto Di Donato
Regiospecificity of two multicomponent monooxygenases from Pseudomonas stutzeri OX1: molecular basis for catabolic adaptation of this microorganism to methylated aromatic compounds.
Applied and environmental microbiology 2005;71(8):4736-43.
2005: Viviana Izzo; Eugenio Notomista; Alessandra Picardi; Francesca Pennacchio; Alberto Di Donato
The thermophilic archaeon Sulfolobus solfataricus is able to grow on phenol.
Research in microbiology 2005;156(5-6):677-89.
2005: Antonello Merlino; Lelio Mazzarella; Anna Carannante; Anna Di Fiore; Alberto Di Donato; Eugenio Notomista; Filomena Sica
The importance of dynamic effects on the enzyme activity: X-ray structure and molecular dynamics of onconase mutants.
The Journal of biological chemistry 2005;280(18):17953-60.
2005: Serena Leone; Viviana Izzo; Rosa Lanzetta; Antonio Molinaro; Michelangelo Parrilli; Alberto Di Donato
The structure of the O-polysaccharide from Pseudomonas stutzeri OX1 containing two different 4-acylamido-4,6-dideoxy-residues, tomosamine and perosamine.
Carbohydrate research 2005;340(4):651-6.
2004: Ambra Viggiani; Loredana Siani; Eugenio Notomista; Leila Birolo; Piero Pucci; Alberto Di Donato
The role of the conserved residues His-246, His-199, and Tyr-255 in the catalysis of catechol 2,3-dioxygenase from Pseudomonas stutzeri OX1.
The Journal of biological chemistry 2004;279(47):48630-9.
2004: Serena Leone; Viviana Izzo; Luisa Sturiale; Domenico Garozzo; Rosa Lanzetta; Michelangelo Parrilli; Antonio Molinaro; Alberto Di Donato
Structure of minor oligosaccharides from the lipopolysaccharide fraction from Pseudomonas stutzeri OX1.
Carbohydrate research 2004;339(16):2657-65.
2004: Matthew H Sazinsky; Joel Bard; Alberto Di Donato; Stephen J Lippard
Crystal structure of the toluene/o-xylene monooxygenase hydroxylase from Pseudomonas stutzeri OX1. Insight into the substrate specificity, substrate channeling, and active site tuning of multicomponent monooxygenases.
The Journal of biological chemistry 2004;279(29):30600-10.
2004: Serena Leone; Viviana Izzo; alba silipo; Luisa Sturiale; Domenico Garozzo; Rosa Lanzetta; Michelangelo Parrilli; Antonio Molinaro; Alberto Di Donato
A novel type of highly negatively charged lipooligosaccharide from Pseudomonas stutzeri OX1 possessing two 4,6-O-(1-carboxy)-ethylidene residues in the outer core region.
European journal of biochemistry / FEBS 2004;271(13):2691-704.
2004: Valeria Cafaro; Viviana Izzo; Roberta Scognamiglio; Eugenio Notomista; Paola Capasso; Annarita Casbarra; Piero Pucci; Alberto Di Donato
Phenol hydroxylase and toluene/o-xylene monooxygenase from Pseudomonas stutzeri OX1: interplay between two enzymes.
Applied and environmental microbiology 2004;70(4):2211-9.
2003: Eugenio Notomista; Armin Lahm; Alberto Di Donato; Anna Tramontano
Evolution of bacterial and archaeal multicomponent monooxygenases.
Journal of molecular evolution 2003;56(4):435-45.
2002: Valeria Cafaro; Roberta Scognamiglio; Ambra Viggiani; Viviana Izzo; Irene Passaro; Eugenio Notomista; Fabrizio Dal Piaz; Angela Amoresano; Annarita Casbarra; Piero Pucci; Alberto Di Donato
Expression and purification of the recombinant subunits of toluene/o-xylene monooxygenase and reconstitution of the active complex.
European journal of biochemistry / FEBS 2002;269(22):5689-99.
2001: Eugenio Notomista; Francesca Catanzano; Giuseppe Graziano; Sonia Di Gaetano; Guido Barone; Alberto Di Donato
Contribution of chain termini to the conformational stability and biological activity of onconase.
Biochemistry 2001;40(31):9097-103.
2001: Roberta Scognamiglio; Eugenio Notomista; Paola Barbieri; Piero Pucci; Fabrizio Dal Piaz; A Tramontano; Alberto Di Donato
Conformational analysis of putative regulatory subunit D of the toluene/o-xylene-monooxygenase complex from Pseudomonas stutzeri OX1.
Protein science : a publication of the Protein Society 2001;10(3):482-90.
2001: Roberta Spadaccini; Orlando Crescenzi; Teodorico Tancredi; N De Casamassimi ; Gabriella Saviano; Roberta Scognamiglio; Alberto Di Donato; Piero A Temussi
Solution structure of a sweet protein: NMR study of MNEI, a single chain monellin.
Journal of molecular biology 2001;305(3):505-14.
2001: Giuseppe D'Alessio; Alberto Di Donato; Renata Piccoli; N Russo
Seminal ribonuclease: preparation of natural and recombinant enzyme, quaternary isoforms, isoenzymes, monomeric forms; assay for selective cytotoxicity of the enzyme.
Methods in enzymology 2001;341():248-63.
2000: Eugenio Notomista; Francesca Catanzano; Giuseppe Graziano; Fabrizio Dal Piaz; Guido Barone; Giuseppe D'Alessio; Alberto Di Donato
Onconase: an unusually stable protein.
Biochemistry 2000;39(30):8711-8.
1999: Eugenio Notomista; Valeria Cafaro; R Fusiello; Aurora Bracale; Giuseppe D'Alessio; Alberto Di Donato
Effective expression and purification of recombinant onconase, an antitumor protein.
FEBS letters 1999;463(3):211-5.
1999: A el-Joubary; Marta Bruix; Jorge Santoro; Valeria Cafaro; Roberta Scognamiglio; Alberto Di Donato; Giuseppe D'Alessio; Katalin E Kövér; Gyula Batta; L Szilágyi; Manuel Rico
1H and 15N sequential assignment and solution secondary structure of 15N labelled human pancreatic ribonuclease.
Journal of biomolecular NMR 1999;15(3):265-6.
1998: Valeria Cafaro; Aurora Bracale; Fabio Formiggini; Eugenio Notomista; Giuseppe D'Alessio; Alberto Di Donato
Protein engineering of ribonucleases.
Biochimie 1998;80(11):905-9.
1998: Francesca Catanzano; Giuseppe Graziano; Valeria Cafaro; Giuseppe D'Alessio; Alberto Di Donato; Guido Barone
Circular dichroism study of ribonuclease A mutants containing the minimal structural requirements for dimerization and swapping.
International journal of biological macromolecules 1998;23(4):277-85.
1998: F Del Vecchio Blanco; Valeria Cafaro; Antimo Di Maro; Roberta Scognamiglio; G Siniscalco; Augusto Parente; Alberto Di Donato
A recombinant ribosome-inactivating protein from the plant Phytolacca dioica L. produced from a synthetic gene.
FEBS letters 1998;437(3):241-5.
1998: Valeria Cafaro; Aurora Bracale; Antimo Di Maro; Salvatore Sorrentino; Giuseppe D'Alessio; Alberto Di Donato
New muteins of RNase A with enhanced antitumor action.
FEBS letters 1998;437(1-2):149-52.
1997: Francesca Catanzano; Giuseppe Graziano; Valeria Cafaro; Giuseppe D'Alessio; Alberto Di Donato; Guido Barone
From ribonuclease A toward bovine seminal ribonuclease: a step by step thermodynamic analysis.
Biochemistry 1997;36(47):14403-8.
1996: N Russo; V Nobile; Alberto Di Donato; James F Riordan; Bert L Vallee
The C-terminal region of human angiogenin has a dual role in enzymatic activity.
Proceedings of the National Academy of Sciences of the United States of America 1996;93(8):3243-7.
1996: S Capasso; Alberto Di Donato; Luciana Esposito; Filomena Sica; Giosué Sorrentino; Luigi Vitagliano; Adriana Zagari; Lelio Mazzarella
Deamidation in proteins: the crystal structure of bovine pancreatic ribonuclease with an isoaspartyl residue at position 67.
Journal of molecular biology 1996;257(3):492-6.
1995: Bianca Stella Adinolfi; Valeria Cafaro; Giuseppe D'Alessio; Alberto Di Donato
Full antitumor action of recombinant seminal ribonuclease depends on the removal of its N-terminal methionine.
Biochemical and biophysical research communications 1995;213(2):525-32.
1995: N Russo; M de Nigris; A Ciardiello; Alberto Di Donato; Giuseppe D'Alessio
Expression in mammalian cells, purification and characterization of recombinant human pancreatic ribonuclease.
FEBS letters 1995;369(2-3):352.
1995: Alberto Di Donato; Valeria Cafaro; I Romeo; Giuseppe D'Alessio
Hints on the evolutionary design of a dimeric RNase with special bioactions.
Protein science : a publication of the Protein Society 1995;4(8):1470-7.
1995: Valeria Cafaro; Claudia De Lorenzo; Renata Piccoli; Aurora Bracale; M R Mastronicola; Alberto Di Donato; Giuseppe D'Alessio
The antitumor action of seminal ribonuclease and its quaternary conformations.
FEBS letters 1995;359(1):31-4.
1994: Giacinto Libertini; Alberto Di Donato
Reconstruction of ancestral sequences by the inferential method, a tool for protein engineering studies.
Journal of molecular evolution 1994;39(2):219-29.
1994: Alberto Di Donato; Valeria Cafaro; Giuseppe D'Alessio
Ribonuclease A can be transformed into a dimeric ribonuclease with antitumor activity.
The Journal of biological chemistry 1994;269(26):17394-6.
1993: N Russo; M de Nigris; A Ciardiello; Alberto Di Donato; Giuseppe D'Alessio
Expression in mammalian cells, purification and characterization of recombinant human pancreatic ribonuclease.
FEBS letters 1993;333(3):233-7.
1993: Alberto Di Donato; Valeria Cafaro; M de Nigris; M Rizzo; Giuseppe D'Alessio
The determinants of the dimeric structure of seminal ribonuclease are located in its N-terminal region.
Biochemical and biophysical research communications 1993;194(3):1440-5.
1993: Alberto Di Donato; M de Nigris; N Russo; S Di Biase; Giuseppe D'Alessio
A method for synthesizing genes and cDNAs by the polymerase chain reaction.
Analytical biochemistry 1993;212(1):291-3.
1993: M de Nigris; N Russo; Renata Piccoli; Giuseppe D'Alessio; Alberto Di Donato
Expression of bovine seminal ribonuclease in Escherichia coli.
Biochemical and biophysical research communications 1993;193(1):155-60.
1993: N Russo; M De Nigris; Alberto Di Donato; Giuseppe D'Alessio
Expression of native dimers of bovine seminal ribonuclease in a eukaryotic cell system.
FEBS letters 1993;318(3):242-4.
1993: Alberto Di Donato; M A Ciardiello; M de Nigris; Renata Piccoli; Lelio Mazzarella; Giuseppe D'Alessio
Selective deamidation of ribonuclease A. Isolation and characterization of the resulting isoaspartyl and aspartyl derivatives.
The Journal of biological chemistry 1993;268(7):4745-51.
1992: Giacinto Libertini; Alberto Di Donato
Computer-aided gene design.
Protein engineering 1992;5(8):821-5.
1992: Renata Piccoli; M Tamburrini; gennaro Piccialli; Alberto Di Donato; Augusto Parente; Giuseppe D'Alessio
The dual-mode quaternary structure of seminal RNase.
Proceedings of the National Academy of Sciences of the United States of America 1992;89(5):1870-4.
1991: Giuseppe D'Alessio; Alberto Di Donato; Augusto Parente; Renata Piccoli
Seminal RNase: a unique member of the ribonuclease superfamily.
Trends in biochemical sciences 1991;16(3):104-6.
1989: M Tamburrini; Renata Piccoli; Delia Picone; Alberto Di Donato; Giuseppe D'Alessio
Dissociation and reconstitution of bovine seminal RNAase: construction of a hyperactive hybrid dimer.
Journal of protein chemistry 1989;8(6):719-31.
1989: R Ambrosino; Guido Barone; G Castronuovo; O Cultrera; Alberto Di Donato; V Elia
A calorimetric approach to the study of the interactions of cytidine-3'-phosphate with bovine seminal ribonuclease.
Biopolymers 1989;28(8):1403-11.
1989: M Merola; A Martínez del Pozo; H Ueno; P Recsei; Alberto Di Donato; James M Manning; K Tanizawa; Y Masu; S Asano; H Tanaka
Site-directed mutagenesis of the cysteinyl residues and the active-site serine residue of bacterial D-amino acid transaminase.
Biochemistry 1989;28(2):505-9.
1988: Renata Piccoli; Alberto Di Donato; Giuseppe D'Alessio
Co-operativity in seminal ribonuclease function. Kinetic studies.
The Biochemical journal 1988;253(2):329-36.
1988: Patrizia Galletti; A Ciardiello; Diego Ingrosso; Alberto Di Donato; Giuseppe D'Alessio
Repair of isopeptide bonds by protein carboxyl O-methyltransferase: seminal ribonuclease as a model system.
Biochemistry 1988;27(5):1752-7.
1987: W J Fantl; Alberto Di Donato; James M Manning; Paul H Rogers; Arthur Arnone
Specifically carboxymethylated hemoglobin as an analogue of carbamino hemoglobin. Solution and X-ray studies of carboxymethylated hemoglobin and X-ray studies of carbamino hemoglobin.
The Journal of biological chemistry 1987;262(26):12700-13.
1987: W J Fantl; Lois R Manning; H Ueno; Alberto Di Donato; James M Manning
Properties of carboxymethylated cross-linked hemoglobin A.
Biochemistry 1987;26(18):5755-61.
1987: Alberto Di Donato; Renata Piccoli; Giuseppe D'Alessio
Co-operativity in seminal ribonuclease function: binding studies.
The Biochemical journal 1987;241(2):435-40.
1986: Alberto Di Donato; Patrizia Galletti; Giuseppe D'Alessio
Selective deamidation and enzymatic methylation of seminal ribonuclease.
Biochemistry 1986;25(26):8361-8.
1986: S Donadio; M Tamburrini; Alberto Di Donato; Renata Piccoli; Giuseppe D'Alessio
Site-directed alkylation and site-site interactions in bovine seminal ribonuclease.
European journal of biochemistry / FEBS 1986;157(3):475-80.
1986: M Tamburrini; Renata Piccoli; R De Prisco; Alberto Di Donato; Giuseppe D'Alessio
Fast and high-yielding procedures for the isolation of bovine seminal RNAase.
The Italian journal of biochemistry 1986;35(1):22-32.
1983: Seetharama A Acharya; Alberto Di Donato; Belur N Manjula; Vincent Fischetti; J M Manning
Influence of trifluoroacetic acid on retention times of histidine-containing tryptic peptides in reverse phase HPLC.
International journal of peptide and protein research 1983;22(1):78-82.
1983: Alberto Di Donato; R Fiore; A M Garzillo; G Marino
Interaction of AMP with cytosolic apo-aspartate aminotransferase.
FEBS letters 1983;153(1):98-102.
1983: S Andini; G D'Alessio; Alberto Di Donato; Livio Paolillo; Renata Piccoli; E Trivellone
Comparative proton NMR studies of bovine semen and pancreas ribonucleases.
Biochimica et biophysica acta 1983;742(3):530-8.
1982: Renata Piccoli; Alberto Di Donato; S Dudkin; Giuseppe D'Alessio
Bovine seminal ribonuclease: non-hyperbolic kinetics in the second reaction strep.
FEBS letters 1982;140(2):307-10.
1981: Alberto Di Donato; Giuseppe D'Alessio
Heterogeneity of bovine seminal ribonuclease.
Biochemistry 1981;20(25):7232-7.
1981: G D'Alessio; Alberto Di Donato; A Furia; E Leone; Massimo Libonati; Augusto Parente; H Suzuki
Bull semen RNAase revisited.
Journal of molecular biology 1981;146(2):269-74.
1979: Alberto Di Donato; G D'Alessio
Intrachain disulfide bridges of bovine seminal ribonuclease.
Biochimica et biophysica acta 1979;579(2):303-13.
1975: M Libonati; S Sorrentino; R Galli; R La Montagna; Alberto Di Donato
Degradation of DNA . RNA hybrids by aggregates of pancreatic ribonuclease.
Biochimica et biophysica acta 1975;407(3):292-8.
1973: Alberto Di Donato; G D'Alessio
Interchain disulfide bridges in ribonuclease BS-1.
Biochemical and biophysical research communications 1973;55(3):919-28.