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Bas Leeflang

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Sander S van Leeuwen; Bas Leeflang; Gerrit J Gerwig; Johannis P Kamerling
Development of a 1H NMR structural-reporter-group concept for the primary structural characterisation of alpha-D-glucans.
Véronique Blanchard; Rupali A Gadkari; Albert V E George; Satarupa Roy; Gerrit J Gerwig; Bas Leeflang; Rajan R Dighe; Rolf Boelens; Johannis P Kamerling
High-level expression of biologically active glycoprotein hormones in Pichia pastoris strains--selection of strain GS115, and not X-33, for the production of biologically active N-glycosylated 15N-labeled phCG.
Véronique Blanchard; Martin Frank; Bas Leeflang; Rolf Boelens; Johannis P Kamerling
The structural basis of the difference in sensitivity for PNGase F in the de-N-glycosylation of the native bovine pancreatic ribonucleases B and BS.

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All Publications

2008: Sander S van Leeuwen; Bas Leeflang; Gerrit J Gerwig; Johannis P Kamerling
Development of a 1H NMR structural-reporter-group concept for the primary structural characterisation of alpha-D-glucans.
Carbohydrate research 2008;343(6):1114-9.
2008: Véronique Blanchard; Rupali A Gadkari; Albert V E George; Satarupa Roy; Gerrit J Gerwig; Bas Leeflang; Rajan R Dighe; Rolf Boelens; Johannis P Kamerling
High-level expression of biologically active glycoprotein hormones in Pichia pastoris strains--selection of strain GS115, and not X-33, for the production of biologically active N-glycosylated 15N-labeled phCG.
Glycoconjugate journal 2008;25(3):245-57.
2008: Véronique Blanchard; Martin Frank; Bas Leeflang; Rolf Boelens; Johannis P Kamerling
The structural basis of the difference in sensitivity for PNGase F in the de-N-glycosylation of the native bovine pancreatic ribonucleases B and BS.
Biochemistry 2008;47(11):3435-46.
2007: Susanne P Jongen; Gerrit J Gerwig; Bas Leeflang; Kate Koles; Maurice L M Mannesse; Patrick H C Van Berkel; Frank R Pieper; Marian A Kroos; Arnold J J Reuser; Qun Zhou; Xiaoying Jin; Kate Zhang; Tim Edmunds; Johannis P Kamerling
N-glycans of recombinant human acid alpha-glucosidase expressed in the milk of transgenic rabbits.
Glycobiology 2007;17(6):600-19.
2007: Véronique Blanchard; Franck Chevalier; Anne Imberty; Bas Leeflang; Kazuyuki Sugahara; Johannis P Kamerling
Conformational studies on five octasaccharides isolated from chondroitin sulfate using NMR spectroscopy and molecular modeling.
Biochemistry 2007;46(5):1167-75.
2007: Gerrit van Meer; Bas Leeflang; Gerhard Liebisch; Gerd Schmitz; Felix M Goñi
The European lipidomics initiative: enabling technologies.
Methods in enzymology 2007;432():213-32.
2007: Véronique Blanchard; Rupali A Gadkari; Gerrit J Gerwig; Bas Leeflang; Rajan R Dighe; Johannis P Kamerling
Characterization of the N-linked oligosaccharides from human chorionic gonadotropin expressed in the methylotrophic yeast Pichia pastoris.
Glycoconjugate journal 2007;24(1):33-47.
2006: E Ab; A R Atkinson; L Banci; I Bertini; S Ciofi-Baffoni; K Brunner; T Diercks; V Dötsch; F Engelke; G E Folkers; C Griesinger; W Gronwald; U Günther; M Habeck; R N de Jong; H R Kalbitzer; B Kieffer; Bas Leeflang; S Loss; Claudio Luchinat; T Marquardsen; D Moskau; K P Neidig; Michael Nilges; M Piccioli; Roberta Pierattelli; W Rieping; T Schippmann; H Schwalbe; G Travé; J Trenner; J Wöhnert; M Zweckstetter; R Kaptein
NMR in the SPINE Structural Proteomics project.
Acta crystallographica. Section D, Biological crystallography 2006;62(Pt 10):1150-61.
2002: Mario van der Stelt; J Albert van Kuik; Monica Bari; Guus van Zadelhoff; Bas Leeflang; Gerrit A Veldink; Alessandro Finazzi-Agrò; Johannes Vliegenthart; Mauro Maccarrone
Oxygenated metabolites of anandamide and 2-arachidonoylglycerol: conformational analysis and interaction with cannabinoid receptors, membrane transporter, and fatty acid amide hydrolase.
Journal of medicinal chemistry 2002;45(17):3709-20.
2002: Gerrit J Gerwig; J Albert van Kuik; Bas Leeflang; Johannis P Kamerling; Johannes Vliegenthart; Craig D Karr; Edward L Jarroll
The Giardia intestinalis filamentous cyst wall contains a novel beta(1-3)-N-acetyl-D-galactosamine polymer: a structural and conformational study.
Glycobiology 2002;12(8):499-505.
2000: Bas Leeflang; Elisabeth J Faber; Paul J A Erbel; Johannes Vliegenthart
Structure elucidation of glycoprotein glycans and of polysaccharides by NMR spectroscopy.
Journal of biotechnology 2000;77(1):115-22.
2000: J L Jiménez Blanco; Johannes J M van Rooijen; Paul J A Erbel; Bas Leeflang; Johannis P Kamerling; Johannes Vliegenthart
Conformational analysis of the Sd(a) determinant-containing tetrasaccharide and two mimics in aqueous solution by using 1H NMR ROESY spectroscopy in combination with MD simulations.
Journal of biomolecular NMR 2000;16(1):59-77.
1998: C W van Zuylen; T de Beer; Bas Leeflang; Rolf Boelens; Robert Kaptein; Johannis P Kamerling; Johannes Vliegenthart
Mobilities of the inner three core residues and the Man(alpha 1--6) branch of the glycan at Asn78 of the alpha-subunit of human chorionic gonadotropin are restricted by the protein.
Biochemistry 1998;37(7):1933-40.
1996: T De Beer; C W Van Zuylen; Bas Leeflang; K Hård; Rolf Boelens; Robert Kaptein; Johannis P Kamerling; Johannes Vliegenthart
NMR studies of the free alpha subunit of human chorionic gonadotropin. Structural influences of N-glycosylation and the beta subunit on the conformation of the alpha subunit.
European journal of biochemistry / FEBS 1996;241(1):229-42.
1996: A J Van den Berg; S F Horsten; J Jantina Kettenes-van den Bosch; CJ Beukelman; B H Kroes; Bas Leeflang; R P Labadie
Podacycline A and B, two cyclic peptides in the latex of Jatropha podagrica.
Phytochemistry 1996;42(1):129-33.
1996: S F Horsten; A J van den Berg; J Jantina Kettenes-van den Bosch; Bas Leeflang; R P Labadie
Cyclogossine A: a novel cyclic heptapeptide isolated from the latex of Jatropha gossypifolia.
Planta medica 1996;62(1):46-50.
1995: A J van den Berg; S F Horsten; J Jantina Kettenes-van den Bosch; B H Kroes; CJ Beukelman; Bas Leeflang; R P Labadie
Curcacycline A--a novel cyclic octapeptide isolated from the latex of Jatropha curcas L.
FEBS letters 1995;358(3):215-8.
1993: L M Kroon-Batenburg; Jan Kroon; Bas Leeflang
Conformational analysis of methyl beta-cellobioside by ROESY NMR spectroscopy and MD simulations in combination with the CROSREL method.
Carbohydrate research 1993;245(1):21-42.
1993: J W Timmermans; P de Waard; H Tournois; Bas Leeflang; Johannes Vliegenthart
NMR spectroscopy of nystose.
Carbohydrate research 1993;243(2):379-84.
1993: M Gruter; Bas Leeflang; J Kuiper; Johannis P Kamerling; Johannes Vliegenthart
Structural characterisation of the exopolysaccharide produced by Lactobacillus delbrückii subspecies bulgaricus rr grown in skimmed milk.
Carbohydrate research 1993;239():209-26.
1992: M Gruter; Bas Leeflang; J Kuiper; Johannis P Kamerling; Johannes Vliegenthart
Structure of the exopolysaccharide produced by Lactococcus lactis subspecies cremoris H414 grown in a defined medium or skimmed milk.
Carbohydrate research 1992;231():273-91.
1992: Bas Leeflang; Johannes Vliegenthart; L M Kroon-Batenburg; B P van Eijck; Jan Kroon
A 1H-NMR and MD study of intramolecular hydrogen bonds in methyl beta-cellobioside.
Carbohydrate research 1992;230(1):41-61.
1992: P de Waard; Bas Leeflang; Johannes Vliegenthart; Rolf Boelens; Geerten W Vuister; Robert Kaptein
Application of 2D and 3D NMR experiments to the conformational study of a diantennary oligosaccharide.
Journal of biomolecular NMR 1992;2(3):211-26.
1991: R A Hoffmann; Bas Leeflang; M M de Barse; Johannis P Kamerling; Johannes Vliegenthart
Characterisation by 1H-n.m.r. spectroscopy of oligosaccharides, derived from arabinoxylans of white endosperm of wheat, that contain the elements ----4)[alpha-L-Araf-(1----3)]-beta-D-Xylp-(1---- or ----4)[alpha- L-Araf-(1----2)][alpha-L-Araf-(1----3)]-beta-D-Xylp-(1----.
Carbohydrate research 1991;221():63-81.
1990: Bas Leeflang; J B Bouwstra; Janos Kerékgyártó; Johannis P Kamerling; Johannes Vliegenthart
ROESY studies and HSEA calculations on xylose-containing oligosaccharides related to N-glycoproteins.
Carbohydrate research 1990;208():117-26.
1990: J B Bouwstra; E C Spoelstra; P De Waard; Bas Leeflang; Johannis P Kamerling; Johannes Vliegenthart
Conformational studies on the N-linked carbohydrate chain of bromelain.
European journal of biochemistry / FEBS 1990;190(1):113-22.