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Stewart Shuman

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TOP 3
Mihaela-Carmen Unciuleac; Stewart Shuman
Distinctive Effects of Domain Deletions on the Manganese-Dependent DNA Polymerase and DNA Phosphorylase Activities of Mycobacterium smegmatis Polynucleotide Phosphorylase.
Lyudmila Yakovleva; Stewart Shuman
Chemical mutagenesis of vaccinia DNA topoisomerase lysine 167 provides insights to the catalysis of DNA transesterification.
Richa Gupta; Sergey Korolev; Olga Koroleva; Mikhail Ryzhikov; Stewart Shuman; Mihaela Unciuleac; Michael S Glickman
A dual role for mycobacterial RecO in RecA-dependent homologous recombination and RecA-independent single-strand annealing.

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All Publications

2013: Mihaela-Carmen Unciuleac; Stewart Shuman
Distinctive Effects of Domain Deletions on the Manganese-Dependent DNA Polymerase and DNA Phosphorylase Activities of Mycobacterium smegmatis Polynucleotide Phosphorylase.
Biochemistry 2013;52(17):2967-81.
2013: Lyudmila Yakovleva; Stewart Shuman
Chemical mutagenesis of vaccinia DNA topoisomerase lysine 167 provides insights to the catalysis of DNA transesterification.
Biochemistry 2013;52(5):984-91.
2013: Richa Gupta; Sergey Korolev; Olga Koroleva; Mikhail Ryzhikov; Stewart Shuman; Mihaela Unciuleac; Michael S Glickman
A dual role for mycobacterial RecO in RecA-dependent homologous recombination and RecA-independent single-strand annealing.
Nucleic acids research 2013;41(4):2284-95.
2013: Ushati Das; Stewart Shuman
Mechanism of RNA 2',3'-cyclic phosphate end healing by T4 polynucleotide kinase-phosphatase.
Nucleic acids research 2013;41(1):355-65.
2012: Hui Zhu; Hitesh Bhattarai; Stewart Shuman; Han-Guang Yan; Michael S Glickman
Characterization of Mycobacterium smegmatis PolD2 and PolD1 as RNA/DNA polymerases homologous to the POL domain of bacterial DNA ligase D.
Biochemistry 2012;51(51):10147-58.
2012: Beate Schwer; Ana M Sanchez; Stewart Shuman
Punctuation and syntax of the RNA polymerase II CTD code in fission yeast.
Proceedings of the National Academy of Sciences of the United States of America 2012;109(44):18024-9.
2012: Anupam K Chakravarty; Stewart Shuman
The sequential 2',3'-cyclic phosphodiesterase and 3'-phosphate/5'-OH ligation steps of the RtcB RNA splicing pathway are GTP-dependent.
Nucleic acids research 2012;40(17):8558-67.
2012: Poulami Samai; Stewart Shuman
Kinetic analysis of DNA strand joining by Chlorella virus DNA ligase and the role of nucleotidyltransferase motif VI in ligase adenylylation.
The Journal of biological chemistry 2012;287(34):28609-18.
2012: Lyudmila Yakovleva; Stewart Shuman
Mycobacterium smegmatis SftH exemplifies a distinctive clade of superfamily II DNA-dependent ATPases with 3' to 5' translocase and helicase activities.
Nucleic acids research 2012;40(15):7465-75.
2012: Jonathan Chang; Beate Schwer; Stewart Shuman
Structure-function analysis and genetic interactions of the yeast branchpoint binding protein Msl5.
Nucleic acids research 2012;40(10):4539-52.
2012: Heather Ordonez; Mihaela Unciuleac; Stewart Shuman
Mycobacterium smegmatis RqlH defines a novel clade of bacterial RecQ-like DNA helicases with ATP-dependent 3'-5' translocase and duplex unwinding activities.
Nucleic acids research 2012;40(10):4604-14.
2012: Anupam K Chakravarty; Brian T Chait; Roman Subbotin; Stewart Shuman
RNA ligase RtcB splices 3'-phosphate and 5'-OH ends via covalent RtcB-(histidinyl)-GMP and polynucleotide-(3')pp(5')G intermediates.
Proceedings of the National Academy of Sciences of the United States of America 2012;109(16):6072-7.
2012: Aswin Natarajan; Stewart Shuman; Deniz B Temel; Kaushik Dutta; Pravin A Nair; Ranajeet Ghose
Solution structure and DNA-binding properties of the phosphoesterase domain of DNA ligase D.
Nucleic acids research 2012;40(5):2076-88.
2012: Paul Smith; Li Kai Wang; Pravin A Nair; Stewart Shuman
The adenylyltransferase domain of bacterial Pnkp defines a unique RNA ligase family.
Proceedings of the National Academy of Sciences of the United States of America 2012;109(7):2296-301.
2012: Hua Cao; Richard C Condit; Peihong Dai; Chee-Mun Fang; Alan N Houghton; Hao Li; Jia Liu; Grant McFadden; Taha Merghoub; PAULA PITHA-ROWE; Stewart Shuman; Fangjin Wang; Weiyi Wang; James W Young; Jianda Yuan; Liang Deng
Innate immune response of human plasmacytoid dendritic cells to poxvirus infection is subverted by vaccinia E3 via its Z-DNA/RNA binding domain.
PloS one 2012;7(5):e36823.
2012: Ushati Das; Paul Smith; Stewart Shuman
Structural insights to the metal specificity of an archaeal member of the LigD 3'-phosphoesterase DNA repair enzyme family.
Nucleic acids research 2012;40(2):828-36.
2011: Peihong Dai; Francesca Avogadri; Hua Cao; Chee-Mun Fang; Katherine A Fitzgerald; Alan N Houghton; Xiaoyu Hu; Grant McFadden; Taha Merghoub; Tanvi Parikh; PAULA PITHA-ROWE; Masmudur M Rahman; Stewart Shuman; Weiyi Wang; Liang Deng
Myxoma virus induces type I interferon production in murine plasmacytoid dendritic cells via a TLR9/MyD88-, IRF5/IRF7-, and IFNAR-dependent pathway.
Journal of virology 2011;85(20):10814-25.
2011: Kaushik Dutta; Pravin A Nair; Aswin Natarajan; Stewart Shuman; Ranajeet Ghose
Sequence-specific 1H, 13C and 15N assignments of the phosphoesterase (PE) domain of Pseudomonas aeruginosa DNA ligase D (LigD).
Biomolecular NMR assignments 2011;5(2):151-5.
2011: Naoko Tanaka; Birthe Meineke; Stewart Shuman
RtcB, a novel RNA ligase, can catalyze tRNA splicing and HAC1 mRNA splicing in vivo.
The Journal of biological chemistry 2011;286(35):30253-7.
2011: Beate Schwer; Hediye Erdjument-Bromage; Stewart Shuman
Composition of yeast snRNPs and snoRNPs in the absence of trimethylguanosine caps reveals nuclear cap binding protein as a gained U1 component implicated in the cold-sensitivity of tgs1Δ cells.
Nucleic acids research 2011;39(15):6715-28.
2011: Beate Schwer; Stewart Shuman
Deciphering the RNA polymerase II CTD code in fission yeast.
Molecular cell 2011;43(2):311-8.
2011: Agnidipta Ghosh; Stewart Shuman; Christopher D Lima
Structural insights to how mammalian capping enzyme reads the CTD code.
Molecular cell 2011;43(2):299-310.
2011: Zhicheng R Qiu; Stewart Shuman; Beate Schwer
An essential role for trimethylguanosine RNA caps in Saccharomyces cerevisiae meiosis and their requirement for splicing of SAE3 and PCH2 meiotic pre-mRNAs.
Nucleic acids research 2011;39(13):5633-46.
2011: Poulami Samai; Stewart Shuman
Structure-function analysis of the OB and latch domains of chlorella virus DNA ligase.
The Journal of biological chemistry 2011;286(25):22642-52.
2011: Poulami Samai; Stewart Shuman
Functional dissection of the DNA interface of the nucleotidyltransferase domain of chlorella virus DNA ligase.
The Journal of biological chemistry 2011;286(15):13314-26.
2011: Paul Smith; Pravin A Nair; Ushati Das; Hui Zhu; Stewart Shuman
Structures and activities of archaeal members of the LigD 3'-phosphoesterase DNA repair enzyme superfamily.
Nucleic acids research 2011;39(8):3310-20.
2011: Zhicheng R Qiu; Beate Schwer; Stewart Shuman
Determinants of Nam8-dependent splicing of meiotic pre-mRNAs.
Nucleic acids research 2011;39(8):3427-45.
2011: Naoko Tanaka; Stewart Shuman
RtcB is the RNA ligase component of an Escherichia coli RNA repair operon.
The Journal of biological chemistry 2011;286(10):7727-31.
2011: Anupam K Chakravarty; Stewart Shuman
RNA 3'-phosphate cyclase (RtcA) catalyzes ligase-like adenylylation of DNA and RNA 5'-monophosphate ends.
The Journal of biological chemistry 2011;286(6):4117-22.
2011: Richa Gupta; Daniel Barkan; Gil Redelman-Sidi; Stewart Shuman; Michael S Glickman
Mycobacteria exploit three genetically distinct DNA double-strand break repair pathways.
Molecular microbiology 2011;79(2):316-30.
2011: Birthe Meineke; Beate Schwer; Raffael Schaffrath; Stewart Shuman
Determinants of eukaryal cell killing by the bacterial ribotoxin PrrC.
Nucleic acids research 2011;39(2):687-700.
2010: Poulami Samai; Paul Smith; Stewart Shuman
Structure of a CRISPR-associated protein Cas2 from Desulfovibrio vulgaris.
Acta crystallographica. Section F, Structural biology and crystallization communications 2010;66(Pt 12):1552-6.
2010: Mihaela-Carmen Unciuleac; Stewart Shuman
Double strand break unwinding and resection by the mycobacterial helicase-nuclease AdnAB in the presence of single strand DNA-binding protein (SSB).
The Journal of biological chemistry 2010;285(45):34319-29.
2010: Daniel A Koster; Aurélien Crut; Stewart Shuman; Mary-Ann Bjornsti; Nynke H Dekker
Cellular strategies for regulating DNA supercoiling: a single-molecule perspective.
Cell 2010;142(4):519-30.
2010: Pravin A Nair; Paul Smith; Stewart Shuman
Structure of bacterial LigD 3'-phosphoesterase unveils a DNA repair superfamily.
Proceedings of the National Academy of Sciences of the United States of America 2010;107(29):12822-7.
2010: Asmita Patel; Lyudmila Yakovleva; Stewart Shuman; Alfonso Mondragón
Crystal structure of a bacterial topoisomerase IB in complex with DNA reveals a secondary DNA binding site.
Structure (London, England : 1993) 2010;18(6):725-33.
2010: Jonathan Chang; Beate Schwer; Stewart Shuman
Mutational analyses of trimethylguanosine synthase (Tgs1) and Mud2: proteins implicated in pre-mRNA splicing.
RNA (New York, N.Y.) 2010;16(5):1018-31.
2010: Susanne Schneider; Yi Pei; Stewart Shuman; Beate Schwer
Separable functions of the fission yeast Spt5 carboxyl-terminal domain (CTD) in capping enzyme binding and transcription elongation overlap with those of the RNA polymerase II CTD.
Molecular and cellular biology 2010;30(10):2353-64.
2010: Naoko Tanaka; Paul Smith; Stewart Shuman
Structure of the RNA 3'-phosphate cyclase-adenylate intermediate illuminates nucleotide specificity and covalent nucleotidyl transfer.
Structure (London, England : 1993) 2010;18(4):449-57.
2010: Li Kai Wang; Stewart Shuman
Mutational analysis of the 5'-OH oligonucleotide phosphate acceptor site of T4 polynucleotide kinase.
Nucleic acids research 2010;38(4):1304-11.
2010: Hui Zhu; Stewart Shuman
Gap filling activities of Pseudomonas DNA ligase D (LigD) polymerase and functional interactions of LigD with the DNA end-binding Ku protein.
The Journal of biological chemistry 2010;285(7):4815-25.
2010: Ruchi Jain; Stewart Shuman
Bacterial Hen1 is a 3' terminal RNA ribose 2'-O-methyltransferase component of a bacterial RNA repair cassette.
RNA (New York, N.Y.) 2010;16(2):316-23.
2010: Mihaela-Carmen Unciuleac; Stewart Shuman
Characterization of the mycobacterial AdnAB DNA motor provides insights into the evolution of bacterial motor-nuclease machines.
The Journal of biological chemistry 2010;285(4):2632-41.
2010: Andrea Piserchio; Pravin A Nair; Stewart Shuman; Ranajeet Ghose
Solution NMR studies of Chlorella virus DNA ligase-adenylate.
Journal of molecular biology 2010;395(2):291-308.
2010: Delphine Benarroch; Marzena Jankowska-Anyszka; Janusz Stepinski; Edward Darzynkiewicz; Stewart Shuman
Cap analog substrates reveal three clades of cap guanine-N2 methyltransferases with distinct methyl acceptor specificities.
RNA (New York, N.Y.) 2010;16(1):211-20.
2009: Naoko Tanaka; Stewart Shuman
Structure-activity relationships in human RNA 3'-phosphate cyclase.
RNA (New York, N.Y.) 2009;15(10):1865-74.
2009: Beate Schwer; Susanne Schneider; Yi Pei; Anna Aronova; Stewart Shuman
Characterization of the Schizosaccharomyces pombe Spt5-Spt4 complex.
RNA (New York, N.Y.) 2009;15(7):1241-50.
2009: Stewart Shuman
DNA ligases: progress and prospects.
The Journal of biological chemistry 2009;284(26):17365-9.
2009: Krishna Murari Sinha; Mihaela-Carmen Unciuleac; Michael S Glickman; Stewart Shuman
AdnAB: a new DSB-resecting motor-nuclease from mycobacteria.
Genes & development 2009;23(12):1423-37.
2009: Andrea Piserchio; Pravin A Nair; Stewart Shuman; Ranajeet Ghose
Sequence-specific 1H N, 13C, and 15N backbone resonance assignments of the 34 kDa Paramecium bursaria Chlorella virus 1 (PBCV1) DNA ligase.
Biomolecular NMR assignments 2009;3(1):77-80.
2009: Niroshika Keppetipola; Ruchi Jain; Birthe Meineke; Melinda Diver; Stewart Shuman
Structure-activity relationships in Kluyveromyces lactis gamma-toxin, a eukaryal tRNA anticodon nuclease.
RNA (New York, N.Y.) 2009;15(6):1036-44.
2009: Krishna Murari Sinha; Michael S Glickman; Stewart Shuman
Mutational analysis of Mycobacterium UvrD1 identifies functional groups required for ATP hydrolysis, DNA unwinding, and chemomechanical coupling.
Biochemistry 2009;48(19):4019-30.
2009: Ruchi Jain; Stewart Shuman
Characterization of a thermostable archaeal polynucleotide kinase homologous to human Clp1.
RNA (New York, N.Y.) 2009;15(5):923-31.
2009: Delphine Benarroch; Zhicheng R Qiu; Beate Schwer; Stewart Shuman
Characterization of a mimivirus RNA cap guanine-N2 methyltransferase.
RNA (New York, N.Y.) 2009;15(4):666-74.
2009: Li Kai Wang; Hui Zhu; Stewart Shuman
Structure-guided Mutational Analysis of the Nucleotidyltransferase Domain of Escherichia coli DNA Ligase (LigA).
The Journal of biological chemistry 2009;284(13):8486-94.
2008: Agnidipta Ghosh; Stewart Shuman; Christopher D Lima
The structure of Fcp1, an essential RNA polymerase II CTD phosphatase.
Molecular cell 2008;32(4):478-90.
2008: Stéphane Hausmann; Sushuang Zheng; Michael Costanzo; Renee L Brost; Dominique Garcin; Charles Boone; Stewart Shuman; Beate Schwer
Genetic and biochemical analysis of yeast and human cap trimethylguanosine synthase: functional overlap of 2,2,7-trimethylguanosine caps, small nuclear ribonucleoprotein components, pre-mRNA splicing factors, and RNA decay pathways.
The Journal of biological chemistry 2008;283(46):31706-18.
2008: Ruchi Jain; Stewart Shuman
Polyphosphatase activity of CthTTM, a bacterial triphosphate tunnel metalloenzyme.
The Journal of biological chemistry 2008;283(45):31047-57.
2008: Niroshika Keppetipola; Stewart Shuman
A phosphate-binding histidine of binuclear metallophosphodiesterase enzymes is a determinant of 2',3'-cyclic nucleotide phosphodiesterase activity.
The Journal of biological chemistry 2008;283(45):30942-9.
2008: Sushuang Zheng; Stewart Shuman
Mutational analysis of vaccinia virus mRNA cap (guanine-N7) methyltransferase reveals essential contributions of the N-terminal peptide that closes over the active site.
RNA (New York, N.Y.) 2008;14(11):2297-304.
2008: Liang Deng; Peihong Dai; Tanvi Parikh; Hua Cao; Vijay Bhoj; Qinmiao Sun; Zhijian J Chen; Taha Merghoub; Alan N Houghton; Stewart Shuman
Vaccinia virus subverts a mitochondrial antiviral signaling protein-dependent innate immune response in keratinocytes through its double-stranded RNA binding protein, E3.
Journal of virology 2008;82(21):10735-46.
2008: Krishna Murari Sinha; Nicolas C Stephanou; Mihaela-Carmen Unciuleac; Michael S Glickman; Stewart Shuman
Domain requirements for DNA unwinding by mycobacterial UvrD2, an essential DNA helicase.
Biochemistry 2008;47(36):9355-64.
2008: Alejandro Ramirez; Stewart Shuman; Beate Schwer
Human RNA 5'-kinase (hClp1) can function as a tRNA splicing enzyme in vivo.
RNA (New York, N.Y.) 2008;14(9):1737-45.
2008: Li Kai Wang; Pravin A Nair; Stewart Shuman
Structure-guided mutational analysis of the OB, HhH, and BRCT domains of Escherichia coli DNA ligase.
The Journal of biological chemistry 2008;283(34):23343-52.
2008: Jayakrishnan Nandakumar; Beate Schwer; Raffael Schaffrath; Stewart Shuman
RNA repair: an antidote to cytotoxic eukaryal RNA damage.
Molecular cell 2008;31(2):278-86.
2008: Lyudmila Yakovleva; Shengxi Chen; Sidney Hecht; Stewart Shuman
Chemical and traditional mutagenesis of vaccinia DNA topoisomerase provides insights to cleavage site recognition and transesterification chemistry.
The Journal of biological chemistry 2008;283(23):16093-103.
2008: Aurélien Crut; Pravin A Nair; Daniel A Koster; Stewart Shuman; Nynke H Dekker
Dynamics of phosphodiester synthesis by DNA ligase.
Proceedings of the National Academy of Sciences of the United States of America 2008;105(19):6894-9.
2008: Delphine Benarroch; Paul Smith; Stewart Shuman
Characterization of a trifunctional mimivirus mRNA capping enzyme and crystal structure of the RNA triphosphatase domain.
Structure (London, England : 1993) 2008;16(4):501-12.
2008: Sushuang Zheng; Stewart Shuman
Structure-function analysis of vaccinia virus mRNA cap (guanine-N7) methyltransferase.
RNA (New York, N.Y.) 2008;14(4):696-705.
2008: Hui Zhu; Stewart Shuman
Bacterial nonhomologous end joining ligases preferentially seal breaks with a 3'-OH monoribonucleotide.
The Journal of biological chemistry 2008;283(13):8331-9.
2008: Jideofor Aniukwu; Michael S Glickman; Stewart Shuman
The pathways and outcomes of mycobacterial NHEJ depend on the structure of the broken DNA ends.
Genes & development 2008;22(4):512-27.
2008: Beate Schwer; Anna Aronova; Alejandro Ramirez; Peter Braun; Stewart Shuman
Mammalian 2',3' cyclic nucleotide phosphodiesterase (CNP) can function as a tRNA splicing enzyme in vivo.
RNA (New York, N.Y.) 2008;14(2):204-10.
2007: Liang Deng; Peihong Dai; Anthony Ciro; Donald F Smee; Hakim Djaballah; Stewart Shuman
Identification of novel antipoxviral agents: mitoxantrone inhibits vaccinia virus replication by blocking virion assembly.
Journal of virology 2007;81(24):13392-402.
2007: Stewart Shuman; Michael S Glickman
Bacterial DNA repair by non-homologous end joining.
Nature reviews. Microbiology 2007;5(11):852-61.
2007: Hui Zhu; Paul Smith; Li Kai Wang; Stewart Shuman
Structure-function analysis of the 3' phosphatase component of T4 polynucleotide kinase/phosphatase.
Virology 2007;366(1):126-36.
2007: Stewart Shuman
Transcriptional networking cap-tures the 7SK RNA 5'-gamma-methyltransferase.
Molecular cell 2007;27(4):517-9.
2007: Pravin A Nair; Jayakrishnan Nandakumar; Paul Smith; Mark Odell; Christopher D Lima; Stewart Shuman
Structural basis for nick recognition by a minimal pluripotent DNA ligase.
Nature structural & molecular biology 2007;14(8):770-8.
2007: Li Kai Wang; Jayakrishnan Nandakumar; Beate Schwer; Stewart Shuman
The C-terminal domain of T4 RNA ligase 1 confers specificity for tRNA repair.
RNA (New York, N.Y.) 2007;13(8):1235-44.
2007: Nicolas C Stephanou; Feng Gao; Paola Bongiorno; Sabine Ehrt; Dirk Schnappinger; Stewart Shuman; Michael S Glickman
Mycobacterial nonhomologous end joining mediates mutagenic repair of chromosomal double-strand DNA breaks.
Journal of bacteriology 2007;189(14):5237-46.
2007: Krishna Murari Sinha; Nicolas C Stephanou; Feng Gao; Michael S Glickman; Stewart Shuman
Mycobacterial UvrD1 is a Ku-dependent DNA helicase that plays a role in multiple DNA repair events, including double-strand break repair.
The Journal of biological chemistry 2007;282(20):15114-25.
2007: Jayakrishnan Nandakumar; Pravin A Nair; Stewart Shuman
Last stop on the road to repair: structure of E. coli DNA ligase bound to nicked DNA-adenylate.
Molecular cell 2007;26(2):257-71.
2007: Niroshika Keppetipola; Ruchi Jain; Stewart Shuman
Novel triphosphate phosphohydrolase activity of Clostridium thermocellum TTM, a member of the triphosphate tunnel metalloenzyme superfamily.
The Journal of biological chemistry 2007;282(16):11941-9.
2007: Ligeng Tian; Stewart Shuman
Vaccinia topoisomerase mutants illuminate roles for Phe59, Gly73, Gln69 and Phe215.
Virology 2007;359(2):466-76.
2007: Niroshika Keppetipola; Stewart Shuman
Characterization of the 2',3' cyclic phosphodiesterase activities of Clostridium thermocellum polynucleotide kinase-phosphatase and bacteriophage lambda phosphatase.
Nucleic acids research 2007;35(22):7721-32.
2007: Sushuang Zheng; Stewart Shuman; Beate Schwer
Sinefungin resistance of Saccharomyces cerevisiae arising from Sam3 mutations that inactivate the AdoMet transporter or from increased expression of AdoMet synthase plus mRNA cap guanine-N7 methyltransferase.
Nucleic acids research 2007;35(20):6895-903.
2007: Niroshika Keppetipola; Jayakrishnan Nandakumar; Stewart Shuman
Reprogramming the tRNA-splicing activity of a bacterial RNA repair enzyme.
Nucleic acids research 2007;35(11):3624-30.
2007: Hui Zhu; Stewart Shuman
Characterization of Agrobacterium tumefaciens DNA ligases C and D.
Nucleic acids research 2007;35(11):3631-45.
2007: Stéphane Hausmann; Alejandro Ramirez; Susanne Schneider; Beate Schwer; Stewart Shuman
Biochemical and genetic analysis of RNA cap guanine-N2 methyltransferases from Giardia lamblia and Schizosaccharomyces pombe.
Nucleic acids research 2007;35(5):1411-20.
2007: Amy C Raymond; Stewart Shuman
Deinococcus radiodurans RNA ligase exemplifies a novel ligase clade with a distinctive N-terminal module that is important for 5'-PO4 nick sealing and ligase adenylylation but dispensable for phosphodiester formation at an adenylylated nick.
Nucleic acids research 2007;35(3):839-49.
2006: Li Kai Wang; Beate Schwer; Stewart Shuman
Structure-guided mutational analysis of T4 RNA ligase 1.
RNA (New York, N.Y.) 2006;12(12):2126-34.
2006: Lyudmila Yakovleva; Jacob Lai; Eric T Kool; Stewart Shuman
Nonpolar nucleobase analogs illuminate requirements for site-specific DNA cleavage by vaccinia topoisomerase.
The Journal of biological chemistry 2006;281(47):35914-21.
2006: Sushuang Zheng; Stéphane Hausmann; Quansheng Liu; Agnidipta Ghosh; Beate Schwer; Christopher D Lima; Stewart Shuman
Mutational analysis of Encephalitozoon cuniculi mRNA cap (guanine-N7) methyltransferase, structure of the enzyme bound to sinefungin, and evidence that cap methyltransferase is the target of sinefungin's antifungal activity.
The Journal of biological chemistry 2006;281(47):35904-13.
2006: Jayakrishnan Nandakumar; Stewart Shuman; Christopher D Lima
RNA ligase structures reveal the basis for RNA specificity and conformational changes that drive ligation forward.
Cell 2006;127(1):71-84.
2006: Liang Deng; Peihong Dai; Wanhong Ding; Richard D Granstein; Stewart Shuman
Vaccinia virus infection attenuates innate immune responses and antigen presentation by epidermal dendritic cells.
Journal of virology 2006;80(20):9977-87.
2006: Delphine Benarroch; Stewart Shuman
Characterization of mimivirus NAD+-dependent DNA ligase.
Virology 2006;353(1):133-43.
2006: Lyudmila Yakovleva; Stewart Shuman
Nucleotide misincorporation, 3'-mismatch extension, and responses to abasic sites and DNA adducts by the polymerase component of bacterial DNA ligase D.
The Journal of biological chemistry 2006;281(35):25026-40.
2006: Beate Schwer; Stewart Shuman
Genetic analysis of poxvirus mRNA cap methyltransferase: suppression of conditional mutations in the stimulatory D12 subunit by second-site mutations in the catalytic D1 subunit.
Virology 2006;352(1):145-56.
2006: Chunling Gong; Paul Smith; Stewart Shuman
Structure-function analysis of Plasmodium RNA triphosphatase and description of a triphosphate tunnel metalloenzyme superfamily that includes Cet1-like RNA triphosphatases and CYTH proteins.
RNA (New York, N.Y.) 2006;12(8):1468-74.
2006: Beate Schwer; Stéphane Hausmann; Susanne Schneider; Stewart Shuman
Poxvirus mRNA cap methyltransferase. Bypass of the requirement for the stimulatory subunit by mutations in the catalytic subunit and evidence for intersubunit allostery.
The Journal of biological chemistry 2006;281(28):18953-60.
2006: Niroshika Keppetipola; Stewart Shuman
Distinct enzymic functional groups are required for the phosphomonoesterase and phosphodiesterase activities of Clostridium thermocellum polynucleotide kinase/phosphatase.
The Journal of biological chemistry 2006;281(28):19251-9.
2006: Lyudmila Yakovleva; Christopher J Handy; Haruhiko Yagi; Jane M Sayer; Donald M Jerina; Stewart Shuman
Intercalating polycyclic aromatic hydrocarbon-DNA adducts poison DNA religation by Vaccinia topoisomerase and act as roadblocks to digestion by exonuclease III.
Biochemistry 2006;45(24):7644-53.
2006: Hui Zhu; Stewart Shuman
Substrate specificity and structure-function analysis of the 3'-phosphoesterase component of the bacterial NHEJ protein, DNA ligase D.
The Journal of biological chemistry 2006;281(20):13873-81.
2006: David L Akey; Alexandra Martins; Jideofor Aniukwu; Michael S Glickman; Stewart Shuman; James M Berger
Crystal structure and nonhomologous end-joining function of the ligase component of Mycobacterium DNA ligase D.
The Journal of biological chemistry 2006;281(19):13412-23.
2006: Asmita Patel; Stewart Shuman; Alfonso Mondragón
Crystal structure of a bacterial type IB DNA topoisomerase reveals a preassembled active site in the absence of DNA.
The Journal of biological chemistry 2006;281(9):6030-7.
2006: Hui Zhu; Jayakrishnan Nandakumar; Jideofor Aniukwu; Li Kai Wang; Michael S Glickman; Christopher D Lima; Stewart Shuman
Atomic structure and nonhomologous end-joining function of the polymerase component of bacterial DNA ligase D.
Proceedings of the National Academy of Sciences of the United States of America 2006;103(6):1711-6.
2006: Yi Pei; Hongyan Du; Juliet Singer; Courtney Stamour; Selena Granitto; Stewart Shuman; Robert P Fisher
Cyclin-dependent kinase 9 (Cdk9) of fission yeast is activated by the CDK-activating kinase Csk1, overlaps functionally with the TFIIH-associated kinase Mcs6, and associates with the mRNA cap methyltransferase Pcm1 in vivo.
Molecular and cellular biology 2006;26(3):777-88.
2006: Li Kai Wang; Beate Schwer; Markus Englert; Hildburg Beier; Stewart Shuman
Structure-function analysis of the kinase-CPD domain of yeast tRNA ligase (Trl1) and requirements for complementation of tRNA splicing by a plant Trl1 homolog.
Nucleic acids research 2006;34(2):517-27.
2006: Delphine Benarroch; Jean-Michel Claverie; Didier Raoult; Stewart Shuman
Characterization of mimivirus DNA topoisomerase IB suggests horizontal gene transfer between eukaryal viruses and bacteria.
Journal of virology 2006;80(1):314-21.
2006: Niroshika Keppetipola; Stewart Shuman
Mechanism of the phosphatase component of Clostridium thermocellum polynucleotide kinase-phosphatase.
RNA (New York, N.Y.) 2006;12(1):73-82.
2005: Man-Hee Suh; Ping Ye; Mincheng Zhang; Stéphane Hausmann; Stewart Shuman; Averell Gnatt; Jianhua Fu
Fcp1 directly recognizes the C-terminal domain (CTD) and interacts with a site on RNA polymerase II distinct from the CTD.
Proceedings of the National Academy of Sciences of the United States of America 2005;102(48):17314-9.
2005: Stéphane Hausmann; Hisashi Koiwa; Shankarling Krishnamurthy; Michael Hampsey; Stewart Shuman
Different strategies for carboxyl-terminal domain (CTD) recognition by serine 5-specific CTD phosphatases.
The Journal of biological chemistry 2005;280(45):37681-8.
2005: Hui Zhu; Li Kai Wang; Stewart Shuman
Essential constituents of the 3'-phosphoesterase domain of bacterial DNA ligase D, a nonhomologous end-joining enzyme.
The Journal of biological chemistry 2005;280(40):33707-15.
2005: Niroshika Keppetipola; Stewart Shuman
Characterization of a thermophilic ATP-dependent DNA ligase from the euryarchaeon Pyrococcus horikoshii.
Journal of bacteriology 2005;187(20):6902-8.
2005: Stéphane Hausmann; Stewart Shuman
Giardia lamblia RNA cap guanine-N2 methyltransferase (Tgs2).
The Journal of biological chemistry 2005;280(37):32101-6.
2005: Alexandra Martins; Stewart Shuman
An end-healing enzyme from Clostridium thermocellum with 5' kinase, 2',3' phosphatase, and adenylyltransferase activities.
RNA (New York, N.Y.) 2005;11(8):1271-80.
2005: Hui Zhu; Stewart Shuman
Novel 3'-ribonuclease and 3'-phosphatase activities of the bacterial non-homologous end-joining protein, DNA ligase D.
The Journal of biological chemistry 2005;280(28):25973-81.
2005: Jayakrishnan Nandakumar; Stewart Shuman
Dual mechanisms whereby a broken RNA end assists the catalysis of its repair by T4 RNA ligase 2.
The Journal of biological chemistry 2005;280(25):23484-9.
2005: Li Kai Wang; Stewart Shuman
Structure-function analysis of yeast tRNA ligase.
RNA (New York, N.Y.) 2005;11(6):966-75.
2005: Stéphane Hausmann; Sushuang Zheng; Carme Fabrega; Stewart W Schneller; Christopher D Lima; Stewart Shuman
Encephalitozoon cuniculi mRNA cap (guanine N-7) methyltransferase: methyl acceptor specificity, inhibition BY S-adenosylmethionine analogs, and structure-guided mutational analysis.
The Journal of biological chemistry 2005;280(21):20404-12.
2005: Anita Changela; Alexandra Martins; Stewart Shuman; Alfonso Mondragón
Crystal structure of baculovirus RNA triphosphatase complexed with phosphate.
The Journal of biological chemistry 2005;280(18):17848-56.
2005: Ligeng Tian; Christopher D Claeboe; Sidney Hecht; Stewart Shuman
Mechanistic plasticity of DNA topoisomerase IB: phosphate electrostatics dictate the need for a catalytic arginine.
Structure (London, England : 1993) 2005;13(4):513-20.
2005: Sébastien Pfeffer; Alain Sewer; Mariana Lagos-Quintana; Robert Sheridan; Chris Sander; Friedrich A Grässer; Linda F van Dyk; Kiong Ho; Stewart Shuman; Minchen Chien; James J Russo; Jingyue Ju; Glenn Randall; Brett D Lindenbach; Charles M Rice; Viviana Simon; David D Ho; Mihaela Zavolan; Thomas Tuschl
Identification of microRNAs of the herpesvirus family.
Nature methods 2005;2(4):269-76.
2005: Chunling Gong; Paola Bongiorno; Alexandra Martins; Nicolas C Stephanou; Hui Zhu; Stewart Shuman; Michael S Glickman
Mechanism of nonhomologous end-joining in mycobacteria: a low-fidelity repair system driven by Ku, ligase D and ligase C.
Nature structural & molecular biology 2005;12(4):304-12.
2005: Hui Zhu; Stewart Shuman
Structure-guided mutational analysis of the nucleotidyltransferase domain of Escherichia coli NAD+-dependent DNA ligase (LigA).
The Journal of biological chemistry 2005;280(13):12137-44.
2005: Stéphane Hausmann; Melissa A Altura; Matthew Witmer; Steven M Singer; Heidi G Elmendorf; Stewart Shuman
Yeast-like mRNA capping apparatus in Giardia lamblia.
The Journal of biological chemistry 2005;280(13):12077-86.
2005: Daniel A Koster; Vincent Croquette; Cees Dekker; Stewart Shuman; Nynke H Dekker
Friction and torque govern the relaxation of DNA supercoils by eukaryotic topoisomerase IB.
Nature 2005;434(7033):671-4.
2005: Stéphane Hausmann; Stewart Shuman
Specificity and mechanism of RNA cap guanine-N2 methyltransferase (Tgs1).
The Journal of biological chemistry 2005;280(6):4021-4.
2005: Hui Zhu; Stewart Shuman
A primer-dependent polymerase function of pseudomonas aeruginosa ATP-dependent DNA ligase (LigD).
The Journal of biological chemistry 2005;280(1):418-27.
2005: M Fahad Khalid; Masad J Damha; Stewart Shuman; Beate Schwer
Structure-function analysis of yeast RNA debranching enzyme (Dbr1), a manganese-dependent phosphodiesterase.
Nucleic acids research 2005;33(19):6349-60.
2005: Fernando Moreno-Herrero; Laurent Holtzer; Daniel A Koster; Stewart Shuman; Cees Dekker; Nynke H Dekker
Atomic force microscopy shows that vaccinia topoisomerase IB generates filaments on DNA in a cooperative fashion.
Nucleic acids research 2005;33(18):5945-53.
2005: Rana Sawaya; Beate Schwer; Stewart Shuman
Structure-function analysis of the yeast NAD+-dependent tRNA 2'-phosphotransferase Tpt1.
RNA (New York, N.Y.) 2005;11(1):107-13.
2004: Alexandra Martins; Stewart Shuman
An RNA ligase from Deinococcus radiodurans.
The Journal of biological chemistry 2004;279(49):50654-61.
2004: Vladimir V Didenko; Candace L Minchew; Stewart Shuman; David S Baskin
Semi-artificial Fluorescent Molecular Machine for DNA Damage Detection.
Nano letters 2004;4(12):2461-6.
2004: Stewart Shuman; Christopher D Lima
The polynucleotide ligase and RNA capping enzyme superfamily of covalent nucleotidyltransferases.
Current opinion in structural biology 2004;14(6):757-64.
2004: Jayakrishnan Nandakumar; Stewart Shuman
How an RNA ligase discriminates RNA versus DNA damage.
Molecular cell 2004;16(2):211-21.
2004: Hisashi Koiwa; Stéphane Hausmann; Woo Young Bang; Akihiro Ueda; Naoko Kondo; Akihiro Hiraguri; Toshiyuki Fukuhara; Jeong Dong Bahk; Dae-Jin Yun; Ray A Bressan; Paul M Hasegawa; Stewart Shuman
Arabidopsis C-terminal domain phosphatase-like 1 and 2 are essential Ser-5-specific C-terminal domain phosphatases.
Proceedings of the National Academy of Sciences of the United States of America 2004;101(40):14539-44.
2004: Ligeng Tian; Jane M Sayer; Donald M Jerina; Stewart Shuman
Individual nucleotide bases, not base pairs, are critical for triggering site-specific DNA cleavage by vaccinia topoisomerase.
The Journal of biological chemistry 2004;279(38):39718-26.
2004: Stewart Shuman
NAD+ specificity of bacterial DNA ligase revealed.
Structure (London, England : 1993) 2004;12(8):1335-6.
2004: Jayakrishnan Nandakumar; Kiong Ho; Christopher D Lima; Stewart Shuman
RNA substrate specificity and structure-guided mutational analysis of bacteriophage T4 RNA ligase 2.
The Journal of biological chemistry 2004;279(30):31337-47.
2004: Hui Zhu; Shenmin Yin; Stewart Shuman
Characterization of polynucleotide kinase/phosphatase enzymes from Mycobacteriophages omega and Cjw1 and vibriophage KVP40.
The Journal of biological chemistry 2004;279(25):26358-69.
2004: Stéphane Hausmann; Beate Schwer; Stewart Shuman
An encephalitozoon cuniculi ortholog of the RNA polymerase II carboxyl-terminal domain (CTD) serine phosphatase Fcp1.
Biochemistry 2004;43(22):7111-20.
2004: Lyudmila Yakovleva; Christopher J Handy; Jane M Sayer; Michael C Pirrung; Donald M Jerina; Stewart Shuman
Benzo[c]phenanthrene adducts and nogalamycin inhibit DNA transesterification by vaccinia topoisomerase.
The Journal of biological chemistry 2004;279(22):23335-42.
2004: Chunling Gong; Alexandra Martins; Paola Bongiorno; Michael S Glickman; Stewart Shuman
Biochemical and genetic analysis of the four DNA ligases of mycobacteria.
The Journal of biological chemistry 2004;279(20):20594-606.
2004: Alexandra Martins; Stewart Shuman
Characterization of a baculovirus enzyme with RNA ligase, polynucleotide 5'-kinase, and polynucleotide 3'-phosphatase activities.
The Journal of biological chemistry 2004;279(18):18220-31.
2004: Stéphane Hausmann; Hediye Erdjument-Bromage; Stewart Shuman
Schizosaccharomyces pombe carboxyl-terminal domain (CTD) phosphatase Fcp1: distributive mechanism, minimal CTD substrate, and active site mapping.
The Journal of biological chemistry 2004;279(12):10892-900.
2004: Beate Schwer; Rana Sawaya; Kiong Ho; Stewart Shuman
Portability and fidelity of RNA-repair systems.
Proceedings of the National Academy of Sciences of the United States of America 2004;101(9):2788-93.
2004: Shiping Liao; Chengde Mao; Jens J Birktoft; Stewart Shuman; Nadrian C Seeman
Resolution of undistorted symmetric immobile DNA junctions by vaccinia topoisomerase I.
Biochemistry 2004;43(6):1520-31.
2004: Stephanie C Schroeder; Diego A R Zorio; Beate Schwer; Stewart Shuman; David Bentley
A function of yeast mRNA cap methyltransferase, Abd1, in transcription by RNA polymerase II.
Molecular cell 2004;13(3):377-87.
2004: Shenmin Yin; C Kiong Ho; Eric S Miller; Stewart Shuman
Characterization of bacteriophage KVP40 and T4 RNA ligase 2.
Virology 2004;319(1):141-51.
2004: Kiong Ho; Li Kai Wang; Christopher D Lima; Stewart Shuman
Structure and mechanism of RNA ligase.
Structure (London, England : 1993) 2004;12(2):327-39.
2004: Carme Fabrega; Stéphane Hausmann; Vincent Shen; Stewart Shuman; Christopher D Lima
Structure and mechanism of mRNA cap (guanine-N7) methyltransferase.
Molecular cell 2004;13(1):77-89.
2004: Ligeng Tian; Christopher D Claeboe; Sidney Hecht; Stewart Shuman
Remote phosphate contacts trigger assembly of the active site of DNA topoisomerase IB.
Structure (London, England : 1993) 2004;12(1):31-40.
2003: Chunling Gong; Alexandra Martins; Stewart Shuman
Structure-function analysis of Trypanosoma brucei RNA triphosphatase and evidence for a two-metal mechanism.
The Journal of biological chemistry 2003;278(51):50843-52.
2003: Rana Sawaya; Beate Schwer; Stewart Shuman
Genetic and biochemical analysis of the functional domains of yeast tRNA ligase.
The Journal of biological chemistry 2003;278(45):43928-38.
2003: Yi Pei; Stewart Shuman
Characterization of the Schizosaccharomyces pombe Cdk9/Pch1 protein kinase: Spt5 phosphorylation, autophosphorylation, and mutational analysis.
The Journal of biological chemistry 2003;278(44):43346-56.
2003: Lyudmila Yakovleva; Ligeng Tian; Jane M Sayer; Govind P Kalena; Heiko Kroth; Donald M Jerina; Stewart Shuman
Site-specific DNA transesterification by vaccinia topoisomerase: effects of benzo[alpha]pyrene-dA, 8-oxoguanine, 8-oxoadenine and 2-aminopurine modifications.
The Journal of biological chemistry 2003;278(43):42170-7.
2003: Mark Odell; Lucy Malinina; Verl Sriskanda; Marianna Teplova; Stewart Shuman
Analysis of the DNA joining repertoire of Chlorella virus DNA ligase and a new crystal structure of the ligase-adenylate intermediate.
Nucleic acids research 2003;31(17):5090-100.
2003: Stéphane Hausmann; Yi Pei; Stewart Shuman
Homodimeric quaternary structure is required for the in vivo function and thermal stability of Saccharomyces cerevisiae and Schizosaccharomyces pombe RNA triphosphatases.
The Journal of biological chemistry 2003;278(33):30487-96.
2003: Li Kai Wang; Kiong Ho; Yi Pei; Stewart Shuman
Mutational analysis of bacteriophage T4 RNA ligase 1. Different functional groups are required for the nucleotidyl transfer and phosphodiester bond formation steps of the ligation reaction.
The Journal of biological chemistry 2003;278(32):29454-62.
2003: Rana Sawaya; Stewart Shuman
Mutational analysis of the guanylyltransferase component of Mammalian mRNA capping enzyme.
Biochemistry 2003;42(27):8240-9.
2003: Ligeng Tian; Christopher D Claeboe; Sidney Hecht; Stewart Shuman
Guarding the genome: electrostatic repulsion of water by DNA suppresses a potent nuclease activity of topoisomerase IB.
Molecular cell 2003;12(1):199-208.
2003: Nayanendu Saha; Stewart Shuman; Beate Schwer
Yeast-based genetic system for functional analysis of poxvirus mRNA cap methyltransferase.
Journal of virology 2003;77(13):7300-7.
2003: Carme Fabrega; Vincent Shen; Stewart Shuman; Christopher D Lima
Structure of an mRNA capping enzyme bound to the phosphorylated carboxy-terminal domain of RNA polymerase II.
Molecular cell 2003;11(6):1549-61.
2003: Shenmin Yin; Kiong Ho; Stewart Shuman
Structure-function analysis of T4 RNA ligase 2.
The Journal of biological chemistry 2003;278(20):17601-8.
2003: Chunling Gong; Stewart Shuman
Mapping the active site of vaccinia virus RNA triphosphatase.
Virology 2003;309(1):125-34.
2003: Stéphane Hausmann; Stewart Shuman
Defining the active site of Schizosaccharomyces pombe C-terminal domain phosphatase Fcp1.
The Journal of biological chemistry 2003;278(16):13627-32.
2003: Ligeng Tian; Jane M Sayer; Heiko Kroth; Govind P Kalena; Donald M Jerina; Stewart Shuman
Benzo[a]pyrene-dG adduct interference illuminates the interface of vaccinia topoisomerase with the DNA minor groove.
The Journal of biological chemistry 2003;278(11):9905-11.
2003: Alexandra Martins; Stewart Shuman
Mapping the triphosphatase active site of baculovirus mRNA capping enzyme LEF4 and evidence for a two-metal mechanism.
Nucleic acids research 2003;31(5):1455-63.
2003: Yi Pei; Beate Schwer; Stewart Shuman
Interactions between fission yeast Cdk9, its cyclin partner Pch1, and mRNA capping enzyme Pct1 suggest an elongation checkpoint for mRNA quality control.
The Journal of biological chemistry 2003;278(9):7180-8.
2002: Alexandra Martins; Stewart Shuman
The domain order of mammalian capping enzyme can be inverted and baculovirus phosphatase can function in cap formation in vivo.
Virology 2002;304(2):167-75.
2002: Alexandra Martins; Stewart Shuman
Mutational analysis of baculovirus phosphatase identifies structural residues important for triphosphatase activity in vitro and in vivo.
Biochemistry 2002;41(45):13403-9.
2002: Kiong Ho; Stewart Shuman
Bacteriophage T4 RNA ligase 2 (gp24.1) exemplifies a family of RNA ligases found in all phylogenetic domains.
Proceedings of the National Academy of Sciences of the United States of America 2002;99(20):12709-14.
2002: Ya-Lin Chiu; Kiong Ho; Nayanendu Saha; Beate Schwer; Stewart Shuman; Tariq M Rana
Tat stimulates cotranscriptional capping of HIV mRNA.
Molecular cell 2002;10(3):585-97.
2002: Stewart Shuman
What messenger RNA capping tells us about eukaryotic evolution.
Nature reviews. Molecular cell biology 2002;3(8):619-25.
2002: Li Kai Wang; Christopher D Lima; Stewart Shuman
Structure and mechanism of T4 polynucleotide kinase: an RNA repair enzyme.
The EMBO journal 2002;21(14):3873-80.
2002: Stéphane Hausmann; Stewart Shuman
Characterization of the CTD phosphatase Fcp1 from fission yeast. Preferential dephosphorylation of serine 2 versus serine 5.
The Journal of biological chemistry 2002;277(24):21213-20.
2002: Yi Pei; Stewart Shuman
Interactions between fission yeast mRNA capping enzymes and elongation factor Spt5.
The Journal of biological chemistry 2002;277(22):19639-48.
2002: Chunling Gong; Stewart Shuman
Chlorella virus RNA triphosphatase. Mutational analysis and mechanism of inhibition by tripolyphosphate.
The Journal of biological chemistry 2002;277(18):15317-24.
2002: Verl Sriskanda; Stewart Shuman
Conserved residues in domain Ia are required for the reaction of Escherichia coli DNA ligase with NAD+.
The Journal of biological chemistry 2002;277(12):9695-700.
2002: Verl Sriskanda; Stewart Shuman
Role of nucleotidyl transferase motif V in strand joining by chlorella virus DNA ligase.
The Journal of biological chemistry 2002;277(12):9661-7.
2002: Berit Olsen Krogh; Stewart Shuman
Proton relay mechanism of general acid catalysis by DNA topoisomerase IB.
The Journal of biological chemistry 2002;277(8):5711-4.
2002: Berit Olsen Krogh; Stewart Shuman
A poxvirus-like type IB topoisomerase family in bacteria.
Proceedings of the National Academy of Sciences of the United States of America 2002;99(4):1853-8.
2002: Li Kai Wang; Stewart Shuman
Mutational analysis defines the 5'-kinase and 3'-phosphatase active sites of T4 polynucleotide kinase.
Nucleic acids research 2002;30(4):1073-80.
2002: Verl Sriskanda; Stewart Shuman
Role of nucleotidyltransferase motifs I, III and IV in the catalysis of phosphodiester bond formation by Chlorella virus DNA ligase.
Nucleic acids research 2002;30(4):903-11.
2002: Stéphane Hausmann; Christian P Vivarès; Stewart Shuman
Characterization of the mRNA capping apparatus of the microsporidian parasite Encephalitozoon cuniculi.
The Journal of biological chemistry 2002;277(1):96-103.
2001: Verl Sriskanda; Stewart Shuman
A second NAD(+)-dependent DNA ligase (LigB) in Escherichia coli.
Nucleic acids research 2001;29(24):4930-4.
2001: Kiong Ho; Stewart Shuman
Trypanosoma brucei RNA triphosphatase. Antiprotozoal drug target and guide to eukaryotic phylogeny.
The Journal of biological chemistry 2001;276(49):46182-6.
2001: Alexandra Martins; Stewart Shuman
Mutational analysis of baculovirus capping enzyme Lef4 delineates an autonomous triphosphatase domain and structural determinants of divalent cation specificity.
The Journal of biological chemistry 2001;276(49):45522-9.
2001: Stéphane Hausmann; Kiong Ho; Beate Schwer; Stewart Shuman
An essential function of Saccharomyces cerevisiae RNA triphosphatase Cet1 is to stabilize RNA guanylyltransferase Ceg1 against thermal inactivation.
The Journal of biological chemistry 2001;276(39):36116-24.
2001: Verl Sriskanda; Richard W Moyer; Stewart Shuman
NAD+-dependent DNA ligase encoded by a eukaryotic virus.
The Journal of biological chemistry 2001;276(39):36100-9.
2001: Berit Olsen Krogh; Stewart Shuman
Vaccinia topoisomerase mutants illuminate conformational changes during closure of the protein clamp and assembly of a functional active site.
The Journal of biological chemistry 2001;276(39):36091-9.
2001: N Saha; Stewart Shuman
Effects of alanine cluster mutations in the D12 subunit of vaccinia virus mRNA (guanine-N7) methyltransferase.
Virology 2001;287(1):40-8.
2001: M Bisaillon; Stewart Shuman
Functional groups required for the stability of yeast RNA triphosphatase in vitro and in vivo.
The Journal of biological chemistry 2001;276(32):30514-20.
2001: Yi Pei; Stéphane Hausmann; Kiong Ho; Beate Schwer; Stewart Shuman
The length, phosphorylation state, and primary structure of the RNA polymerase II carboxyl-terminal domain dictate interactions with mRNA capping enzymes.
The Journal of biological chemistry 2001;276(30):28075-82.
2001: Li Kai Wang; Stewart Shuman
Domain structure and mutational analysis of T4 polynucleotide kinase.
The Journal of biological chemistry 2001;276(29):26868-74.
2001: Berit Olsen Krogh; Christopher D Claeboe; Sidney Hecht; Stewart Shuman
Effect of 2'-5' phosphodiesters on DNA transesterification by vaccinia topoisomerase.
The Journal of biological chemistry 2001;276(24):20907-12.
2001: M Bisaillon; Stewart Shuman
Structure-function analysis of the active site tunnel of yeast RNA triphosphatase.
The Journal of biological chemistry 2001;276(20):17261-6.
2001: Anita Changela; Kiong Ho; A Martins; Stewart Shuman; Alfonso Mondragón
Structure and mechanism of the RNA triphosphatase component of mammalian mRNA capping enzyme.
The EMBO journal 2001;20(10):2575-86.
2001: K Lehman; Kiong Ho; Stewart Shuman
Importance of homodimerization for the in vivo function of yeast RNA triphosphatase.
The Journal of biological chemistry 2001;276(18):14996-5002.
2001: Y L Chiu; E Coronel; Kiong Ho; Stewart Shuman; Tariq M Rana
HIV-1 Tat protein interacts with mammalian capping enzyme and stimulates capping of TAR RNA.
The Journal of biological chemistry 2001;276(16):12959-66.
2001: Y Ramanathan; S M Rajpara; Syed M Reza; E Lees; Stewart Shuman; Michael B Mathews; Tsafi Pe'ery
Three RNA polymerase II carboxyl-terminal domain kinases display distinct substrate preferences.
The Journal of biological chemistry 2001;276(14):10913-20.
2001: Kiong Ho; Stewart Shuman
A yeast-like mRNA capping apparatus in Plasmodium falciparum.
Proceedings of the National Academy of Sciences of the United States of America 2001;98(6):3050-5.
2001: Kiong Ho; Chunling Gong; Stewart Shuman
RNA triphosphatase component of the mRNA capping apparatus of Paramecium bursaria Chlorella virus 1.
Journal of virology 2001;75(4):1744-50.
2001: Beate Schwer; K Lehman; Nayanendu Saha; Stewart Shuman
Characterization of the mRNA capping apparatus of Candida albicans.
The Journal of biological chemistry 2001;276(3):1857-64.
2001: Yi Pei; Beate Schwer; Stéphane Hausmann; Stewart Shuman
Characterization of Schizosaccharomyces pombe RNA triphosphatase.
Nucleic acids research 2001;29(2):387-96.
2001: Eckhard Jankowsky; C H Gross; Stewart Shuman; Anna Marie Pyle
Active disruption of an RNA-protein interaction by a DExH/D RNA helicase.
Science (New York, N.Y.) 2001;291(5501):121-5.
2001: Stewart Shuman
The mRNA capping apparatus as drug target and guide to eukaryotic phylogeny.
Cold Spring Harbor symposia on quantitative biology 2001;66():301-12.
2001: Y Pei; B Schwer; Julia E Saiz; Robert P Fisher; Stewart Shuman
RNA triphosphatase is essential in Schizosaccharomyces pombe and Candida albicans.
BMC microbiology 2001;1():29.
2001: Stewart Shuman
Analysis of topoisomerase-DNA interactions by electrophoretic mobility shift assay.
Methods in molecular biology (Clifton, N.J.) 2001;95():65-74.
2001: Stewart Shuman
Structure, mechanism, and evolution of the mRNA capping apparatus.
Progress in nucleic acid research and molecular biology 2001;66():1-40.
2000: Alexandra Martins; Stewart Shuman
Mechanism of phosphoanhydride cleavage by baculovirus phosphatase.
The Journal of biological chemistry 2000;275(45):35070-6.
2000: M Odell; Verl Sriskanda; Stewart Shuman; Dimitar B Nikolov
Crystal structure of eukaryotic DNA ligase-adenylate illuminates the mechanism of nick sensing and strand joining.
Molecular cell 2000;6(5):1183-93.
2000: Chonghui Cheng; Stewart Shuman
Recombinogenic flap ligation pathway for intrinsic repair of topoisomerase IB-induced double-strand breaks.
Molecular and cellular biology 2000;20(21):8059-68.
2000: S C Schroeder; Beate Schwer; Stewart Shuman; David Bentley
Dynamic association of capping enzymes with transcribing RNA polymerase II.
Genes & development 2000;14(19):2435-40.
2000: G Woodfield; Chonghui Cheng; Stewart Shuman; Alex B Burgin
Vaccinia topoisomerase and Cre recombinase catalyze direct ligation of activated DNA substrates containing a 3'-para-nitrophenyl phosphate ester.
Nucleic acids research 2000;28(17):3323-31.
2000: Beate Schwer; Nayanendu Saha; X Mao; H W Chen; Stewart Shuman
Structure-function analysis of yeast mRNA cap methyltransferase and high-copy suppression of conditional mutants by AdoMet synthase and the ubiquitin conjugating enzyme Cdc34p.
Genetics 2000;155(4):1561-76.
2000: JoAnn Sekiguchi; Chonghui Cheng; Stewart Shuman
Resolution of a Holliday junction by vaccinia topoisomerase requires a spacer DNA segment 3' of the CCCTT/ cleavage sites.
Nucleic acids research 2000;28(14):2658-63.
2000: M Klemm; Chonghui Cheng; Geoffrey D Cassell; Stewart Shuman; Anca M Segall
Peptide inhibitors of DNA cleavage by tyrosine recombinases and topoisomerases.
Journal of molecular biology 2000;299(5):1203-16.
2000: Berit Olsen Krogh; Stewart Shuman
Catalytic mechanism of DNA topoisomerase IB.
Molecular cell 2000;5(6):1035-41.
2000: Verl Sriskanda; Zvi Kelman; Jerard Hurwitz; Stewart Shuman
Characterization of an ATP-dependent DNA ligase from the thermophilic archaeon Methanobacterium thermoautotrophicum.
Nucleic acids research 2000;28(11):2221-8.
2000: Kiong Ho; Alexandra Martins; Stewart Shuman
A yeast-based genetic system for functional analysis of viral mRNA capping enzymes.
Journal of virology 2000;74(12):5486-94.
2000: Berit Olsen Krogh; Stewart Shuman
DNA strand transfer catalyzed by vaccinia topoisomerase: peroxidolysis and hydroxylaminolysis of the covalent protein-DNA intermediate.
Biochemistry 2000;39(21):6422-32.
2000: James T Stivers; G J Jagadeesh; Barbara Nawrot; Wojciech J Stec; Stewart Shuman
Stereochemical outcome and kinetic effects of Rp- and Sp-phosphorothioate substitutions at the cleavage site of vaccinia type I DNA topoisomerase.
Biochemistry 2000;39(18):5561-72.
2000: Chonghui Cheng; Stewart Shuman
DNA strand transfer catalyzed by vaccinia topoisomerase: ligation of DNAs containing a 3' mononucleotide overhang.
Nucleic acids research 2000;28(9):1893-8.
2000: Yi Pei; K Lehman; L Tian; Stewart Shuman
Characterization of Candida albicans RNA triphosphatase and mutational analysis of its active site.
Nucleic acids research 2000;28(9):1885-92.
2000: Eckhard Jankowsky; C H Gross; Stewart Shuman; Anna Marie Pyle
The DExH protein NPH-II is a processive and directional motor for unwinding RNA.
Nature 2000;403(6768):447-51.
1999: Kiong Ho; K Lehman; Stewart Shuman
An essential surface motif (WAQKW) of yeast RNA triphosphatase mediates formation of the mRNA capping enzyme complex with RNA guanylyltransferase.
Nucleic acids research 1999;27(24):4671-8.
1999: Chonghui Cheng; Stewart Shuman
Site-specific DNA transesterification by vaccinia topoisomerase: role of specific phosphates and nucleosides.
Biochemistry 1999;38(50):16599-612.
1999: Berit Olsen Krogh; Chonghui Cheng; Alex B Burgin; Stewart Shuman
Melanoplus sanguinipes entomopoxvirus DNA topoisomerase: site-specific DNA transesterification and effects of 5'-bridging phosphorothiolates.
Virology 1999;264(2):441-51.
1999: Christopher D Lima; Li Kai Wang; Stewart Shuman
Structure and mechanism of yeast RNA triphosphatase: an essential component of the mRNA capping apparatus.
Cell 1999;99(5):533-43.
1999: Verl Sriskanda; Beate Schwer; Kiong Ho; Stewart Shuman
Mutational analysis of Escherichia coli DNA ligase identifies amino acids required for nick-ligation in vitro and for in vivo complementation of the growth of yeast cells deleted for CDC9 and LIG4.
Nucleic acids research 1999;27(20):3953-63.
1999: Yi Pei; Kiong Ho; Beate Schwer; Stewart Shuman
Mutational analyses of yeast RNA triphosphatases highlight a common mechanism of metal-dependent NTP hydrolysis and a means of targeting enzymes to pre-mRNAs in vivo by fusion to the guanylyltransferase component of the capping apparatus.
The Journal of biological chemistry 1999;274(41):28865-74.
1999: K Lehman; Beate Schwer; Kiong Ho; I Rouzankina; Stewart Shuman
A conserved domain of yeast RNA triphosphatase flanking the catalytic core regulates self-association and interaction with the guanylyltransferase component of the mRNA capping apparatus.
The Journal of biological chemistry 1999;274(32):22668-78.
1999: N Saha; Beate Schwer; Stewart Shuman
Characterization of human, Schizosaccharomyces pombe, and Candida albicans mRNA cap methyltransferases and complete replacement of the yeast capping apparatus by mammalian enzymes.
The Journal of biological chemistry 1999;274(23):16553-62.
1999: M Odell; Stewart Shuman
Footprinting of Chlorella virus DNA ligase bound at a nick in duplex DNA.
The Journal of biological chemistry 1999;274(20):14032-9.
1999: Kiong Ho; Stewart Shuman
Distinct roles for CTD Ser-2 and Ser-5 phosphorylation in the recruitment and allosteric activation of mammalian mRNA capping enzyme.
Molecular cell 1999;3(3):405-11.
1999: Alexandra Martins; CH Gross; Stewart Shuman
Mutational analysis of vaccinia virus nucleoside triphosphate phosphohydrolase I, a DNA-dependent ATPase of the DExH box family.
Journal of virology 1999;73(2):1302-8.
1998: Kiong Ho; Yi Pei; Stewart Shuman
Yeast and viral RNA 5' triphosphatases comprise a new nucleoside triphosphatase family.
The Journal of biological chemistry 1998;273(51):34151-6.
1998: CH Gross; Stewart Shuman
RNA 5'-triphosphatase, nucleoside triphosphatase, and guanylyltransferase activities of baculovirus LEF-4 protein.
Journal of virology 1998;72(12):10020-8.
1998: Verl Sriskanda; Stewart Shuman
Mutational analysis of Chlorella virus DNA ligase: catalytic roles of domain I and motif VI.
Nucleic acids research 1998;26(20):4618-25.
1998: Stewart Shuman
Vaccinia virus DNA topoisomerase: a model eukaryotic type IB enzyme.
Biochimica et biophysica acta 1998;1400(1-3):321-37.
1998: Kiong Ho; Beate Schwer; Stewart Shuman
Genetic, physical, and functional interactions between the triphosphatase and guanylyltransferase components of the yeast mRNA capping apparatus.
Molecular and cellular biology 1998;18(9):5189-98.
1998: CH Gross; Stewart Shuman
Characterization of a baculovirus-encoded RNA 5'-triphosphatase.
Journal of virology 1998;72(9):7057-63.
1998: Verl Sriskanda; Stewart Shuman
Specificity and fidelity of strand joining by Chlorella virus DNA ligase.
Nucleic acids research 1998;26(15):3536-41.
1998: CH Gross; Stewart Shuman
The nucleoside triphosphatase and helicase activities of vaccinia virus NPH-II are essential for virus replication.
Journal of virology 1998;72(6):4729-36.
1998: Chonghui Cheng; Stewart Shuman
A catalytic domain of eukaryotic DNA topoisomerase I.
The Journal of biological chemistry 1998;273(19):11589-95.
1998: Beate Schwer; X Mao; Stewart Shuman
Accelerated mRNA decay in conditional mutants of yeast mRNA capping enzyme.
Nucleic acids research 1998;26(9):2050-7.
1998: Kiong Ho; Verl Sriskanda; Susan McCracken; David Bentley; Beate Schwer; Stewart Shuman
The guanylyltransferase domain of mammalian mRNA capping enzyme binds to the phosphorylated carboxyl-terminal domain of RNA polymerase II.
The Journal of biological chemistry 1998;273(16):9577-85.
1998: Stewart Shuman
Polynucleotide ligase activity of eukaryotic topoisomerase I.
Molecular cell 1998;1(5):741-8.
1998: Chonghui Cheng; P Kussie; N Pavletich; Stewart Shuman
Conservation of structure and mechanism between eukaryotic topoisomerase I and site-specific recombinases.
Cell 1998;92(6):841-50.
1998: Liang Deng; Stewart Shuman
Vaccinia NPH-I, a DExH-box ATPase, is the energy coupling factor for mRNA transcription termination.
Genes & development 1998;12(4):538-46.
1998: Beate Schwer; P Linder; Stewart Shuman
Effects of deletion mutations in the yeast Ces1 protein on cell growth and morphology and on high copy suppression of mutations in mRNA capping enzyme and translation initiation factor 4A.
Nucleic acids research 1998;26(3):803-9.
1998: Verl Sriskanda; Stewart Shuman
Chlorella virus DNA ligase: nick recognition and mutational analysis.
Nucleic acids research 1998;26(2):525-31.
1998: J Wittschieben; BO Petersen; Stewart Shuman
Replacement of the active site tyrosine of vaccinia DNA topoisomerase by glutamate, cysteine or histidine converts the enzyme into a site-specific endonuclease.
Nucleic acids research 1998;26(2):490-6.
1998: Susan McCracken; Emanuel Rosonina; Nova Fong; M Sikes; A Beyer; K O'Hare; Stewart Shuman; David Bentley
Role of RNA polymerase II carboxy-terminal domain in coordinating transcription with RNA processing.
Cold Spring Harbor symposia on quantitative biology 1998;63():301-9.
1997: Liang Deng; Stewart Shuman
Elongation properties of vaccinia virus RNA polymerase: pausing, slippage, 3' end addition, and termination site choice.
Biochemistry 1997;36(50):15892-9.
1997: Susan McCracken; Nova Fong; Emanuel Rosonina; Krassimir Yankulov; G Brothers; David Siderovski; A Hessel; S Foster; Stewart Shuman; David L Bentley
5'-Capping enzymes are targeted to pre-mRNA by binding to the phosphorylated carboxy-terminal domain of RNA polymerase II.
Genes & development 1997;11(24):3306-18.
1997: JoAnn Sekiguchi; Stewart Shuman
Site-specific ribonuclease activity of eukaryotic DNA topoisomerase I.
Molecular cell 1997;1(1):89-97.
1997: L Yu; Alexandra Martins; Liang Deng; Stewart Shuman
Structure-function analysis of the triphosphatase component of vaccinia virus mRNA capping enzyme.
Journal of virology 1997;71(12):9837-43.
1997: JoAnn Sekiguchi; Stewart Shuman
Nick sensing by vaccinia virus DNA ligase requires a 5' phosphate at the nick and occupancy of the adenylate binding site on the enzyme.
Journal of virology 1997;71(12):9679-84.
1997: Stewart Shuman
Origins of mRNA identity: capping enzymes bind to the phosphorylated C-terminal domain of RNA polymerase II.
Proceedings of the National Academy of Sciences of the United States of America 1997;94(24):12758-60.
1997: Stewart Shuman; D G Bear; JoAnn Sekiguchi
Intramolecular synapsis of duplex DNA by vaccinia topoisomerase.
The EMBO journal 1997;16(21):6584-9.
1997: JoAnn Sekiguchi; Stewart Shuman
Mutational analysis of vaccinia virus topoisomerase identifies residues involved in DNA binding.
Nucleic acids research 1997;25(18):3649-56.
1997: S P Wang; Liang Deng; Kiong Ho; Stewart Shuman
Phylogeny of mRNA capping enzymes.
Proceedings of the National Academy of Sciences of the United States of America 1997;94(18):9573-8.
1997: JoAnn Sekiguchi; Stewart Shuman
Novobiocin inhibits vaccinia virus replication by blocking virus assembly.
Virology 1997;235(1):129-37.
1997: J Wittschieben; Stewart Shuman
Mechanism of DNA transesterification by vaccinia topoisomerase: catalytic contributions of essential residues Arg-130, Gly-132, Tyr-136 and Lys-167.
Nucleic acids research 1997;25(15):3001-8.
1997: JoAnn Sekiguchi; Stewart Shuman
Ligation of RNA-containing duplexes by vaccinia DNA ligase.
Biochemistry 1997;36(29):9073-9.
1997: AJ Doherty; K Håkansson; Kiong Ho; Stewart Shuman; Dale B Wigley
Crystallization of the RNA guanylyltransferase of Chlorella virus PBCV-1.
Acta crystallographica. Section D, Biological crystallography 1997;53(Pt 4):482-4.
1997: Li Kai Wang; J Wittschieben; Stewart Shuman
Mutational analysis of 26 residues of vaccinia DNA topoisomerase identifies Ser-204 as important for DNA binding and cleavage.
Biochemistry 1997;36(26):7944-50.
1997: JoAnn Sekiguchi; Chonghui Cheng; Stewart Shuman
Kinetic analysis of DNA and RNA strand transfer reactions catalyzed by vaccinia topoisomerase.
The Journal of biological chemistry 1997;272(25):15721-8.
1997: S P Wang; Stewart Shuman
Structure-function analysis of the mRNA cap methyltransferase of Saccharomyces cerevisiae.
The Journal of biological chemistry 1997;272(23):14683-9.
1997: BO Petersen; Stewart Shuman
DNA strand transfer reactions catalyzed by vaccinia topoisomerase: hydrolysis and glycerololysis of the covalent protein-DNA intermediate.
Nucleic acids research 1997;25(11):2091-7.
1997: K Håkansson; AJ Doherty; Stewart Shuman; Dale B Wigley
X-ray crystallography reveals a large conformational change during guanyl transfer by mRNA capping enzymes.
Cell 1997;89(4):545-53.
1997: BO Petersen; Richard L Hall; Richard W Moyer; Stewart Shuman
Characterization of a DNA topoisomerase encoded by Amsacta moore entomopoxvirus.
Virology 1997;230(2):197-206.
1997: Li Kai Wang; Stewart Shuman
Deletions at the carboxyl terminus of vaccinia DNA topoisomerase affect DNA binding and enhance distributivity in DNA relaxation.
Biochemistry 1997;36(13):3909-16.
1997: Chonghui Cheng; Stewart Shuman
Characterization of an ATP-dependent DNA ligase encoded by Haemophilus influenzae.
Nucleic acids research 1997;25(7):1369-74.
1997: Chonghui Cheng; Li Kai Wang; JoAnn Sekiguchi; Stewart Shuman
Mutational analysis of 39 residues of vaccinia DNA topoisomerase identifies Lys-220, Arg-223, and Asn-228 as important for covalent catalysis.
The Journal of biological chemistry 1997;272(13):8263-9.
1997: Kiong Ho; J L Van Etten; Stewart Shuman
Characterization of an ATP-dependent DNA ligase encoded by Chlorella virus PBCV-1.
Journal of virology 1997;71(3):1931-7.
1997: JoAnn Sekiguchi; Stewart Shuman
Domain structure of vaccinia DNA ligase.
Nucleic acids research 1997;25(4):727-34.
1997: BO Petersen; Stewart Shuman
Histidine 265 is important for covalent catalysis by vaccinia topoisomerase and is conserved in all eukaryotic type I enzymes.
The Journal of biological chemistry 1997;272(7):3891-6.
1997: Stewart Shuman
A proposed mechanism of mRNA synthesis and capping by vesicular stomatitis virus.
Virology 1997;227(1):1-6.
1997: Liang Deng; Stewart Shuman
Transcription termination by vaccinia RNA polymerase entails recognition of specific phosphates in the nascent RNA.
The Journal of biological chemistry 1997;272(1):695-8.
1996: CH Gross; Stewart Shuman
Vaccinia virions lacking the RNA helicase nucleoside triphosphate phosphohydrolase II are defective in early transcription.
Journal of virology 1996;70(12):8549-57.
1996: Liang Deng; Stewart Shuman
An ATPase component of the transcription elongation complex is required for factor-dependent transcription termination by vaccinia RNA polymerase.
The Journal of biological chemistry 1996;271(46):29386-92.
1996: BO Petersen; J Wittschieben; Stewart Shuman
Mutations within a conserved region of vaccinia topoisomerase affect the DNA cleavage-religation equilibrium.
Journal of molecular biology 1996;263(2):181-95.
1996: Kiong Ho; J L Van Etten; Stewart Shuman
Expression and characterization of an RNA capping enzyme encoded by Chlorella virus PBCV-1.
Journal of virology 1996;70(10):6658-64.
1996: L Yu; Stewart Shuman
Mutational analysis of the RNA triphosphatase component of vaccinia virus mRNA capping enzyme.
Journal of virology 1996;70(9):6162-8.
1996: Liang Deng; J Hagler; Stewart Shuman
Factor-dependent release of nascent RNA by ternary complexes of vaccinia RNA polymerase.
The Journal of biological chemistry 1996;271(32):19556-62.
1996: JoAnn Sekiguchi; Stewart Shuman
Covalent DNA binding by vaccinia topoisomerase results in unpairing of the thymine base 5' of the scissile bond.
The Journal of biological chemistry 1996;271(32):19436-42.
1996: JoAnn Sekiguchi; Stewart Shuman
Identification of contacts between topoisomerase I and its target DNA by site-specific photocrosslinking.
The EMBO journal 1996;15(13):3448-57.
1996: Stewart Shuman
Closing the gap on DNA ligase.
Structure (London, England : 1993) 1996;4(6):653-6.
1996: B Schwer; Stewart Shuman
Conditional inactivation of mRNA capping enzyme affects yeast pre-mRNA splicing in vivo.
RNA (New York, N.Y.) 1996;2(6):574-83.
1996: X Mao; Stewart Shuman
Vaccinia virus mRNA (guanine-7-)methyltransferase: mutational effects on cap methylation and AdoHcy-dependent photo-cross-linking of the cap to the methyl acceptor site.
Biochemistry 1996;35(21):6900-10.
1996: CH Gross; Stewart Shuman
Vaccinia virus RNA helicase: nucleic acid specificity in duplex unwinding.
Journal of virology 1996;70(4):2615-9.
1996: Kiong Ho; Stewart Shuman
Mutational analysis of the vaccinia virus E3 protein defines amino acid residues involved in E3 binding to double-stranded RNA.
Journal of virology 1996;70(4):2611-4.
1996: CH Gross; Stewart Shuman
The QRxGRxGRxxxG motif of the vaccinia virus DExH box RNA helicase NPH-II is required for ATP hydrolysis and RNA unwinding but not for RNA binding.
Journal of virology 1996;70(3):1706-13.
1996: Kiong Ho; Stewart Shuman
Physical and functional characterization of the double-stranded RNA binding protein encoded by the vaccinia virus E3 gene.
Virology 1996;217(1):272-84.
1996: S P Wang; Stewart Shuman
A temperature-sensitive mutation of the vaccinia virus E11 gene encoding a 15-kDa virion component.
Virology 1996;216(1):252-7.
1996: X Mao; Beate Schwer; Stewart Shuman
Mutational analysis of the Saccharomyces cerevisiae ABD1 gene: cap methyltransferase activity is essential for cell growth.
Molecular and cellular biology 1996;16(2):475-80.
1996: JoAnn Sekiguchi; James T Stivers; A S Mildvan; Stewart Shuman
Mechanism of inhibition of vaccinia DNA topoisomerase by novobiocin and coumermycin.
The Journal of biological chemistry 1996;271(4):2313-22.
1996: JoAnn Sekiguchi; N C Seeman; Stewart Shuman
Resolution of Holliday junctions by eukaryotic DNA topoisomerase I.
Proceedings of the National Academy of Sciences of the United States of America 1996;93(2):785-9.
1996: B Schwer; Stewart Shuman
Multicopy suppressors of temperature-sensitive mutations of yeast mRNA capping enzyme.
Gene expression 1996;5(6):331-44.
1995: Stewart Shuman
Vaccinia virus DNA ligase: specificity, fidelity, and inhibition.
Biochemistry 1995;34(49):16138-47.
1995: P Cong; Stewart Shuman
Mutational analysis of mRNA capping enzyme identifies amino acids involved in GTP binding, enzyme-guanylate formation, and GMP transfer to RNA.
Molecular and cellular biology 1995;15(11):6222-31.
1995: M Ericsson; S Cudmore; Stewart Shuman; R C Condit; Gareth Griffiths; Jacomine Krijnse Locker
Characterization of ts 16, a temperature-sensitive mutant of vaccinia virus.
Journal of virology 1995;69(11):7072-86.
1995: S Wang; Stewart Shuman
Vaccinia virus morphogenesis is blocked by temperature-sensitive mutations in the F10 gene, which encodes protein kinase 2.
Journal of virology 1995;69(10):6376-88.
1995: E M Kane; Stewart Shuman
Adenosine N1-oxide inhibits vaccinia virus replication by blocking translation of viral early mRNAs.
Journal of virology 1995;69(10):6352-8.
1995: Stewart Shuman; Beate Schwer
RNA capping enzyme and DNA ligase: a superfamily of covalent nucleotidyl transferases.
Molecular microbiology 1995;17(3):405-10.
1995: Stewart Shuman; X M Ru
Mutational analysis of vaccinia DNA ligase defines residues essential for covalent catalysis.
Virology 1995;211(1):73-83.
1995: X Mao; Beate Schwer; Stewart Shuman
Yeast mRNA cap methyltransferase is a 50-kilodalton protein encoded by an essential gene.
Molecular and cellular biology 1995;15(8):4167-74.
1995: CH Gross; Stewart Shuman
Mutational analysis of vaccinia virus nucleoside triphosphate phosphohydrolase II, a DExH box RNA helicase.
Journal of virology 1995;69(8):4727-36.
1995: Y Luo; X Mao; Liang Deng; P Cong; Stewart Shuman
The D1 and D12 subunits are both essential for the transcription termination factor activity of vaccinia virus capping enzyme.
Journal of virology 1995;69(6):3852-6.
1995: JoAnn Sekiguchi; Stewart Shuman
Proteolytic footprinting of vaccinia topoisomerase bound to DNA.
The Journal of biological chemistry 1995;270(19):11636-45.
1995: Stewart Shuman
Capping enzyme in eukaryotic mRNA synthesis.
Progress in nucleic acid research and molecular biology 1995;50():101-29.
1994: James T Stivers; Stewart Shuman; Albert S Mildvan
Vaccinia DNA topoisomerase I: kinetic evidence for general acid-base catalysis and a conformational step.
Biochemistry 1994;33(51):15449-58.
1994: Stewart Shuman
Novel approach to molecular cloning and polynucleotide synthesis using vaccinia DNA topoisomerase.
The Journal of biological chemistry 1994;269(51):32678-84.
1994: JoAnn Sekiguchi; Stewart Shuman
Vaccinia topoisomerase binds circumferentially to DNA.
The Journal of biological chemistry 1994;269(50):31731-4.
1994: JoAnn Sekiguchi; Stewart Shuman
Requirements for noncovalent binding of vaccinia topoisomerase I to duplex DNA.
Nucleic acids research 1994;22(24):5360-5.
1994: Stewart Shuman; Y Liu; Beate Schwer
Covalent catalysis in nucleotidyl transfer reactions: essential motifs in Saccharomyces cerevisiae RNA capping enzyme are conserved in Schizosaccharomyces pombe and viral capping enzymes and among polynucleotide ligases.
Proceedings of the National Academy of Sciences of the United States of America 1994;91(25):12046-50.
1994: J Wittschieben; Stewart Shuman
Mutational analysis of vaccinia DNA topoisomerase defines amino acid residues essential for covalent catalysis.
The Journal of biological chemistry 1994;269(47):29978-83.
1994: JoAnn Sekiguchi; Stewart Shuman
Stimulation of vaccinia topoisomerase I by nucleoside triphosphates.
The Journal of biological chemistry 1994;269(47):29760-4.
1994: X Mao; Stewart Shuman
Intrinsic RNA (guanine-7) methyltransferase activity of the vaccinia virus capping enzyme D1 subunit is stimulated by the D12 subunit. Identification of amino acid residues in the D1 protein required for subunit association and methyl group transfer.
The Journal of biological chemistry 1994;269(39):24472-9.
1994: Liang Deng; Stewart Shuman
A role for the H4 subunit of vaccinia RNA polymerase in transcription initiation at a viral early promoter.
The Journal of biological chemistry 1994;269(19):14323-8.
1994: B Schwer; Stewart Shuman
Mutational analysis of yeast mRNA capping enzyme.
Proceedings of the National Academy of Sciences of the United States of America 1994;91(10):4328-32.
1994: J Hagler; Y Luo; Stewart Shuman
Factor-dependent transcription termination by vaccinia RNA polymerase. Kinetic coupling and requirement for ATP hydrolysis.
The Journal of biological chemistry 1994;269(13):10050-60.
1994: James T Stivers; Stewart Shuman; Albert S Mildvan
Vaccinia DNA topoisomerase I: single-turnover and steady-state kinetic analysis of the DNA strand cleavage and ligation reactions.
Biochemistry 1994;33(1):327-39.
1993: Y Luo; Stewart Shuman
RNA binding properties of vaccinia virus capping enzyme.
The Journal of biological chemistry 1993;268(28):21253-62.
1993: Stewart Shuman; J Turner
Site-specific interaction of vaccinia virus topoisomerase I with base and sugar moieties in duplex DNA.
The Journal of biological chemistry 1993;268(25):18943-50.
1993: Stewart Shuman
Vaccinia virus RNA helicase. Directionality and substrate specificity.
The Journal of biological chemistry 1993;268(16):11798-802.
1993: E M Kane; Stewart Shuman
Vaccinia virus morphogenesis is blocked by a temperature-sensitive mutation in the I7 gene that encodes a virion component.
Journal of virology 1993;67(5):2689-98.
1993: P Cong; Stewart Shuman
Covalent catalysis in nucleotidyl transfer. A KTDG motif essential for enzyme-GMP complex formation by mRNA capping enzyme is conserved at the active sites of RNA and DNA ligases.
The Journal of biological chemistry 1993;268(10):7256-60.
1993: J Hagler; Stewart Shuman
Nascent RNA cleavage by purified ternary complexes of vaccinia RNA polymerase.
The Journal of biological chemistry 1993;268(3):2166-73.
1992: Stewart Shuman
Vaccinia virus RNA helicase: an essential enzyme related to the DE-H family of RNA-dependent NTPases.
Proceedings of the National Academy of Sciences of the United States of America 1992;89(22):10935-9.
1992: J Hagler; Stewart Shuman
Structural analysis of ternary complexes of vaccinia RNA polymerase.
Proceedings of the National Academy of Sciences of the United States of America 1992;89(21):10099-103.
1992: E M Kane; Stewart Shuman
Temperature-sensitive mutations in the vaccinia virus H4 gene encoding a component of the virion RNA polymerase.
Journal of virology 1992;66(10):5752-62.
1992: Stewart Shuman
Two classes of DNA end-joining reactions catalyzed by vaccinia topoisomerase I.
The Journal of biological chemistry 1992;267(24):16755-8.
1992: P Cong; Stewart Shuman
Methyltransferase and subunit association domains of vaccinia virus mRNA capping enzyme.
The Journal of biological chemistry 1992;267(23):16424-9.
1992: S G Morham; Stewart Shuman
Covalent and noncovalent DNA binding by mutants of vaccinia DNA topoisomerase I.
The Journal of biological chemistry 1992;267(22):15984-92.
1992: J Hagler; Stewart Shuman
Ternary complex formation by vaccinia virus RNA polymerase at an early viral promoter: analysis by native gel electrophoresis.
Journal of virology 1992;66(5):2982-9.
1992: Stewart Shuman
DNA strand transfer reactions catalyzed by vaccinia topoisomerase I.
The Journal of biological chemistry 1992;267(12):8620-7.
1992: J Hagler; Stewart Shuman
Stability of ternary transcription complexes of vaccinia virus RNA polymerase at promoter-proximal positions.
The Journal of biological chemistry 1992;267(11):7644-54.
1992: J Hagler; Stewart Shuman
A freeze-frame view of eukaryotic transcription during elongation and capping of nascent mRNA.
Science (New York, N.Y.) 1992;255(5047):983-6.
1991: Stewart Shuman
Recombination mediated by vaccinia virus DNA topoisomerase I in Escherichia coli is sequence specific.
Proceedings of the National Academy of Sciences of the United States of America 1991;88(22):10104-8.
1991: Y Luo; Stewart Shuman
Antitermination of vaccinia virus early transcription: possible role of RNA secondary structure.
Virology 1991;185(1):432-6.
1991: Stewart Shuman
Site-specific interaction of vaccinia virus topoisomerase I with duplex DNA. Minimal DNA substrate for strand cleavage in vitro.
The Journal of biological chemistry 1991;266(30):20576-7.
1991: Y Luo; J Hagler; Stewart Shuman
Discrete functional stages of vaccinia virus early transcription during a single round of RNA synthesis in vitro.
The Journal of biological chemistry 1991;266(20):13303-10.
1991: Stewart Shuman
Site-specific interaction of vaccinia virus topoisomerase I with duplex DNA. Minimal DNA substrate for strand cleavage in vitro.
The Journal of biological chemistry 1991;266(17):11372-9.
1991: Stewart Shuman
Site-specific DNA cleavage by vaccinia virus DNA topoisomerase I. Role of nucleotide sequence and DNA secondary structure.
The Journal of biological chemistry 1991;266(3):1796-803.
1990: Stewart Shuman; J Prescott
Specific DNA cleavage and binding by vaccinia virus DNA topoisomerase I.
The Journal of biological chemistry 1990;265(29):17826-36.
1990: Stewart Shuman; S G Morham
Domain structure of vaccinia virus mRNA capping enzyme. Activity of the Mr 95,000 subunit expressed in Escherichia coli.
The Journal of biological chemistry 1990;265(20):11967-72.
1990: Stewart Shuman
Catalytic activity of vaccinia mRNA capping enzyme subunits coexpressed in Escherichia coli.
The Journal of biological chemistry 1990;265(20):11960-6.
1990: S G Morham; Stewart Shuman
Phenotypic selection and characterization of mutant alleles of a eukaryotic DNA topoisomerase I.
Genes & development 1990;4(4):515-24.
1990: Stewart Shuman; Bernard Moss
Purification and use of vaccinia virus messenger RNA capping enzyme.
Methods in enzymology 1990;181():170-80.
1989: Stewart Shuman; Bernard Moss
Bromouridine triphosphate inhibits transcription termination and mRNA release by vaccinia virions.
The Journal of biological chemistry 1989;264(35):21356-60.
1989: Stewart Shuman; E M Kane; S G Morham
Mapping the active-site tyrosine of vaccinia virus DNA topoisomerase I.
Proceedings of the National Academy of Sciences of the United States of America 1989;86(24):9793-7.
1989: E G Niles; G J Lee-Chen; Stewart Shuman; Bernard Moss; S S Broyles
Vaccinia virus gene D12L encodes the small subunit of the viral mRNA capping enzyme.
Virology 1989;172(2):513-22.
1989: Stewart Shuman
Functional domains of vaccinia virus mRNA capping enzyme. Analysis by limited tryptic digestion.
The Journal of biological chemistry 1989;264(16):9690-5.
1989: Stewart Shuman
Vaccinia DNA topoisomerase I promotes illegitimate recombination in Escherichia coli.
Proceedings of the National Academy of Sciences of the United States of America 1989;86(10):3489-93.
1989: Stewart Shuman; M Golder; Bernard Moss
Insertional mutagenesis of the vaccinia virus gene encoding a type I DNA topoisomerase: evidence that the gene is essential for virus growth.
Virology 1989;170(1):302-6.
1988: Stewart Shuman; M Golder; Bernard Moss
Characterization of vaccinia virus DNA topoisomerase I expressed in Escherichia coli.
The Journal of biological chemistry 1988;263(31):16401-7.
1988: S S Broyles; L Yuen; Stewart Shuman; Bernard Moss
Purification of a factor required for transcription of vaccinia virus early genes.
The Journal of biological chemistry 1988;263(22):10754-60.
1988: Stewart Shuman; Bernard Moss
Vaccinia virus poly(A) polymerase. Specificity for nucleotides and nucleotide analogs.
The Journal of biological chemistry 1988;263(17):8405-12.
1988: Stewart Shuman; Bernard Moss
Factor-dependent transcription termination by vaccinia virus RNA polymerase. Evidence that the cis-acting termination signal is in nascent RNA.
The Journal of biological chemistry 1988;263(13):6220-5.
1987: Stewart Shuman; Bernard Moss
Identification of a vaccinia virus gene encoding a type I DNA topoisomerase.
Proceedings of the National Academy of Sciences of the United States of America 1987;84(21):7478-82.
1987: Stewart Shuman; S S Broyles; Bernard Moss
Purification and characterization of a transcription termination factor from vaccinia virions.
The Journal of biological chemistry 1987;262(25):12372-80.
1982: Stewart Shuman
RNA capping by HeLa cell RNA guanylyltransferase. Characterization of a covalent protein-guanylate intermediate.
The Journal of biological chemistry 1982;257(12):7237-45.
1981: Stewart Shuman; Jerard Hurwitz
Mechanism of mRNA capping by vaccinia virus guanylyltransferase: characterization of an enzyme--guanylate intermediate.
Proceedings of the National Academy of Sciences of the United States of America 1981;78(1):187-91.
1980: Stewart Shuman; Martin I Surks; Henry Furneaux; Jerard Hurwitz
Purification and characterization of a GTP-pyrophosphate exchange activity from vaccinia virions. Association of the GTP-pyrophosphate exchange activity with vaccinia mRNA guanylyltransferase . RNA (guanine-7-)methyltransferase complex (capping enzyme).
The Journal of biological chemistry 1980;255(23):11588-98.
1980: E Spencer; Stewart Shuman; Jerard Hurwitz
Purification and properties of vaccinia virus DNA-dependent RNA polymerase.
The Journal of biological chemistry 1980;255(11):5388-95.
1980: Stewart Shuman; E Spencer; Henry Furneaux; Jerard Hurwitz
The role of ATP in in vitro vaccinia virus RNA synthesis effects of AMP-PNP and ATP gamma S.
The Journal of biological chemistry 1980;255(11):5396-403.
1979: Stewart Shuman; Jerard Hurwitz
5'-Hydroxyl polyribonucleotide kinase from HeLa cell nuclei. Purification and properties.
The Journal of biological chemistry 1979;254(20):10396-404.