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Alfred Pingoud

Research Profile

Chemicals & Drugs

Concepts & Ideas

Living Beings

Escherichia coli

Genes & Molecular Sequences

Amino Acid Sequences

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Physiology

Mutagenesis, Site-Directed

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Polymerase Chain Reaction

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Publications

TOP 3
Lilia Gabsalilow; Alfred Pingoud; Benno Schierling; Peter Friedhoff; Wolfgang Wende
Site- and strand-specific nicking of DNA by fusion proteins derived from MutH and I-SceI or TALE repeats.
Eliza J R Peterson; Alfred Pingoud; William P Janzen; Dmitri Kireev; Marika Midon; Andrea F Moon; Lars C Pedersen; Scott Singleton
Inhibitors of Streptococcus pneumoniae surface endonuclease EndA discovered by high-throughput screening using a PicoGreen fluorescence assay.
José M Otero; Alfred Pingoud; Benno Schierling; Pablo Guardado Calvo; Antonio Luis Llamas-Saiz; Ann Josée Noël; Wolfgang Wende; Mark van Raaij
High-resolution structures of Thermus thermophilus enoyl-acyl carrier protein reductase in the apo form, in complex with NAD+ and in complex with NAD+ and triclosan.

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All Publications

2013: Lilia Gabsalilow; Alfred Pingoud; Benno Schierling; Peter Friedhoff; Wolfgang Wende
Site- and strand-specific nicking of DNA by fusion proteins derived from MutH and I-SceI or TALE repeats.
Nucleic acids research 2013;41(7):e83.
2013: Eliza J R Peterson; Alfred Pingoud; William P Janzen; Dmitri Kireev; Marika Midon; Andrea F Moon; Lars C Pedersen; Scott Singleton
Inhibitors of Streptococcus pneumoniae surface endonuclease EndA discovered by high-throughput screening using a PicoGreen fluorescence assay.
Journal of biomolecular screening 2013;18(3):247-57.
2012: José M Otero; Alfred Pingoud; Benno Schierling; Pablo Guardado Calvo; Antonio Luis Llamas-Saiz; Ann Josée Noël; Wolfgang Wende; Mark van Raaij
High-resolution structures of Thermus thermophilus enoyl-acyl carrier protein reductase in the apo form, in complex with NAD+ and in complex with NAD+ and triclosan.
Acta crystallographica. Section F, Structural biology and crystallization communications 2012;68(Pt 10):1139-48.
2012: Daniel Kutscher; Albert Jeltsch; Alfred Pingoud; Gregor Meiss
Identification of ICAD-derived peptides capable of inhibiting caspase-activated DNase.
The FEBS journal 2012;279(16):2917-28.
2012: Benno Schierling; Wolfgang Wende; Alfred Pingoud
Redesigning the single-chain variant of the restriction endonuclease PvuII by circular permutation.
FEBS letters 2012;586(12):1736-41.
2012: Jasmina Dikić; Samuel Clarke; Michael Kokkinidis; Carolin Menges; Alfred Pingoud; Wolfgang Wende; Pierre Desbiolles
The rotation-coupled sliding of EcoRV.
Nucleic acids research 2012;40(9):4064-70.
2012: Marika Midon; Peter Friedhoff; Oleg Gimadutdinow; Gregor Meiss; Alfred Pingoud
Chemical rescue of active site mutants of S. pneumoniae surface endonuclease EndA and other nucleases of the HNH family by imidazole.
Chembiochem : a European journal of chemical biology 2012;13(5):713-21.
2012: Benno Schierling; Wolfgang Wende; Toni Cathomen; Nadine Dannemann; Lilia Gabsalilow; Alfred Pingoud
A novel zinc-finger nuclease platform with a sequence-specific cleavage module.
Nucleic acids research 2012;40(6):2623-38.
2012: Ines Fonfara; Alfred Pingoud; Ute Curth; Wolfgang Wende
Creating highly specific nucleases by fusion of active restriction endonucleases and catalytically inactive homing endonucleases.
Nucleic acids research 2012;40(2):847-60.
2011: Le Thi Hien; Elena A Kubareva; Alfred Pingoud; Benno Schierling; Eugene M Volkov; Wolfgang Wende; Timofei S Zatsepin; Tatiana S Oretskaya
Restriction endonuclease SsoII with photoregulated activity--a "molecular gate" approach.
Bioconjugate chemistry 2011;22(7):1366-73.
2011: Alfred Pingoud; Wolfgang Wende
Generation of novel nucleases with extended specificity by rational and combinatorial strategies.
Chembiochem : a European journal of chemical biology 2011;12(10):1495-500.
2011: Andrea F Moon; Marika Midon; Gregor Meiss; Alfred Pingoud; Robert E London; Lars C Pedersen
Structural insights into catalytic and substrate binding mechanisms of the strategic EndA nuclease from Streptococcus pneumoniae.
Nucleic acids research 2011;39(7):2943-53.
2011: Marika Midon; Patrick Schäfer; Alfred Pingoud; Mahua Ghosh; Andrea F Moon; Matthew J Cuneo; Robert E London; Gregor Meiss
Mutational and biochemical analysis of the DNA-entry nuclease EndA from Streptococcus pneumoniae.
Nucleic acids research 2011;39(2):623-34.
2010: Benno Schierling; Ann-Josée Noël; Wolfgang Wende; Le Thi Hien; Eugeny Volkov; Elena Kubareva; Tatiana Oretskaya; Michael Kokkinidis; Andreas Römpp; Bernhard Spengler; Alfred Pingoud
Controlling the enzymatic activity of a restriction enzyme by light.
Proceedings of the National Academy of Sciences of the United States of America 2010;107(4):1361-6.
2009: Vera Pingoud; Wolfgang Wende; Peter Friedhoff; Monika Reuter; Jürgen Alves; Albert Jeltsch; Letif Mones; Monika Fuxreiter; Alfred Pingoud
On the divalent metal ion dependence of DNA cleavage by restriction endonucleases of the EcoRI family.
Journal of molecular biology 2009;393(1):140-60.
2009: Pawan Singh; Pankaj Tripathi; George H Silva; Alfred Pingoud; K Muniyappa
Characterization of Mycobacterium leprae RecA intein, a LAGLIDADG homing endonuclease, reveals a unique mode of DNA binding, helical distortion, and cleavage compared with a canonical LAGLIDADG homing endonuclease.
The Journal of biological chemistry 2009;284(38):25912-28.
2009: Tessa M Geel; Gregor Meiss; Bernardina T van der Gun; Bart Jan Kroesen; Lou F de Leij; Mindaugas Zaremba; Arūnas Silanskas; Michael Kokkinidis; Alfred Pingoud; Marcel H Ruiters; Pamela M McLaughlin; Marianne G Rots
Endonucleases induced TRAIL-insensitive apoptosis in ovarian carcinoma cells.
Experimental cell research 2009;315(15):2487-95.
2009: Le Tkhi Khien; B Shirling; A Iu Riazanova; Timofei S Zatsepin; Eugeny M Volkov; Elena Kubareva; TI Velichko; Alfred Pingoud; T S Oretskaia
[New derivatives of azobenzene for the directed modification of proteins].
Bioorganicheskaia khimiia 2009;35(5):610-7.
2009: Andreas Biebricher; Wolfgang Wende; Christophe Escudé; Alfred Pingoud; Pierre Desbiolles
Tracking of single quantum dot labeled EcoRV sliding along DNA manipulated by double optical tweezers.
Biophysical journal 2009;96(8):L50-2.
2008: Isabelle Bonnet; Andreas Biebricher; Pierre-Louis Porté; Claude Loverdo; Olivier Bénichou; Raphaël Voituriez; Christophe Escudé; Wolfgang Wende; Alfred Pingoud; Pierre Desbiolles
Sliding and jumping of single EcoRV restriction enzymes on non-cognate DNA.
Nucleic acids research 2008;36(12):4118-27.
2007: Alfred Pingoud; George H Silva
Precision genome surgery.
Nature biotechnology 2007;25(7):743-4.
2007: Alfred Pingoud; Wolfgang Wende
A sliding restriction enzyme pauses.
Structure (London, England : 1993) 2007;15(4):391-3.
2007: Mahua Ghosh; Gregor Meiss; Alfred Pingoud; Robert E London; Lars C Pedersen
The nuclease a-inhibitor complex is characterized by a novel metal ion bridge.
The Journal of biological chemistry 2007;282(8):5682-90.
2007: Philipp Rathert; Tamás Raskó; Markus Roth; Krystyna Slaska-Kiss; Alfred Pingoud; Antal Kiss; Albert Jeltsch
Reversible inactivation of the CG specific SssI DNA (cytosine-C5)-methyltransferase with a photocleavable protecting group.
Chembiochem : a European journal of chemical biology 2007;8(2):202-7.
2007: Peter Friedhoff; Alfred Pingoud
Engineering site-specific endonucleases.
Methods in molecular biology (Clifton, N.J.) 2007;352():111-23.
2006: George H Silva; Marlene Belfort; Wolfgang Wende; Alfred Pingoud
From monomeric to homodimeric endonucleases and back: engineering novel specificity of LAGLIDADG enzymes.
Journal of molecular biology 2006;361(4):744-54.
2005: A E Sud'ina; Timofei S Zatsepin; Vera Pingoud; Alfred Pingoud; Tatiana S Oretskaya; Elena Kubareva
Affinity modification of the restriction endonuclease SsoII by 2'-aldehyde-containing double stranded DNAs.
Biochemistry. Biokhimii͡a 2005;70(8):941-7.
2005: Mahua Ghosh; Gregor Meiss; Alfred Pingoud; Robert E London; Lars C Pedersen
Structural insights into the mechanism of nuclease A, a betabeta alpha metal nuclease from Anabaena.
The Journal of biological chemistry 2005;280(30):27990-7.
2005: Vera Pingoud; Hildegard Geyer; Rudolf Geyer; Elena Kubareva; Janusz Bujnicki; Alfred Pingoud
Identification of base-specific contacts in protein-DNA complexes by photocrosslinking and mass spectrometry: a case study using the restriction endonuclease SsoII.
Molecular bioSystems 2005;1(2):135-41.
2005: Alfred Pingoud; M Fuxreiter; Vera Pingoud; Wolfgang Wende
Type II restriction endonucleases: structure and mechanism.
Cellular and molecular life sciences : CMLS 2005;62(6):685-707.
2005: Christian Korn; Sebastian R Scholz; Oleg Gimadutdinow; Rudi Lurz; Alfred Pingoud; Gregor Meiss
Interaction of DNA fragmentation factor (DFF) with DNA reveals an unprecedented mechanism for nuclease inhibition and suggests that DFF can be activated in a DNA-bound state.
The Journal of biological chemistry 2005;280(7):6005-15.
2005: Vera Pingoud; Anna Sudina; Hildegard Geyer; Janusz Bujnicki; Rudi Lurz; Gerhild Lüder; Richard Morgan; Elena Kubareva; Alfred Pingoud
Specificity changes in the evolution of type II restriction endonucleases: a biochemical and bioinformatic analysis of restriction enzymes that recognize unrelated sequences.
The Journal of biological chemistry 2005;280(6):4289-98.
2005: Kristin Eisenschmidt; Thomas Lanio; András Simoncsits; Albert Jeltsch; Vera Pingoud; Wolfgang Wende; Alfred Pingoud
Developing a programmed restriction endonuclease for highly specific DNA cleavage.
Nucleic acids research 2005;33(22):7039-47.
2004: Patrick Schäfer; Sebastian R Scholz; Oleg Gimadutdinow; Iwona A Cymerman; Janusz Bujnicki; Adolf Ruiz-Carrillo; Alfred Pingoud; Gregor Meiss
Structural and functional characterization of mitochondrial EndoG, a sugar non-specific nuclease which plays an important role during apoptosis.
Journal of molecular biology 2004;338(2):217-28.
2004: Ann-Josée Noël; Wolfgang Wende; Alfred Pingoud
DNA recognition by the homing endonuclease PI-SceI involves a divalent metal ion cofactor-induced conformational change.
The Journal of biological chemistry 2004;279(8):6794-804.
2004: Shawn Steuer; Vera Pingoud; Alfred Pingoud; Wolfgang Wende
Chimeras of the homing endonuclease PI-SceI and the homologous Candida tropicalis intein: a study to explore the possibility of exchanging DNA-binding modules to obtain highly specific endonucleases with altered specificity.
Chembiochem : a European journal of chemical biology 2004;5(2):206-13.
2003: Jochen Wilhelm; Alfred Pingoud
Real-time polymerase chain reaction.
Chembiochem : a European journal of chemical biology 2003;4(11):1120-8.
2003: Björn Tews; Jochen Wilhelm; Daniel Summerer; Michael Strerath; Andreas Marx; Peter Friedhoff; Alfred Pingoud; Meinhard Hahn
Application of the C4'-alkylated deoxyribose primer system (CAPS) in allele-specific real-time PCR for increased selectivity in discrimination of single nucleotide sequence variants.
Biological chemistry 2003;384(10-11):1533-41.
2003: Sebastian R Scholz; Christian Korn; Janusz Bujnicki; Oleg Gimadutdinow; Alfred Pingoud; Gregor Meiss
Experimental evidence for a beta beta alpha-Me-finger nuclease motif to represent the active site of the caspase-activated DNase.
Biochemistry 2003;42(31):9288-94.
2003: Aspasia Spyridaki; Claudia Matzen; Thomas Lanio; Albert Jeltsch; András Simoncsits; Alekos Athanasiadis; Eva Scheuring-Vanamee; Michael Kokkinidis; Alfred Pingoud
Structural and biochemical characterization of a new Mg(2+) binding site near Tyr94 in the restriction endonuclease PvuII.
Journal of molecular biology 2003;331(2):395-406.
2003: Vera Pingoud; Charlotte Conzelmann; Steffen Kinzebach; Anna Sudina; Valeri Metelev; Elena Kubareva; Janusz Bujnicki; Rudi Lurz; Gerhild Lüder; Shuang-Yong Xu; Alfred Pingoud
PspGI, a type II restriction endonuclease from the extreme thermophile Pyrococcus sp.: structural and functional studies to investigate an evolutionary relationship with several mesophilic restriction enzymes.
Journal of molecular biology 2003;329(5):913-29.
2003: Jochen Wilhelm; Alfred Pingoud; Meinhard Hahn
Validation of an algorithm for automatic quantification of nucleic acid copy numbers by real-time polymerase chain reaction.
Analytical biochemistry 2003;317(2):218-25.
2003: Jochen Wilhelm; Alfred Pingoud; Meinhard Hahn
Real-time PCR-based method for the estimation of genome sizes.
Nucleic acids research 2003;31(10):e56.
2003: Richard J Roberts; Marlene Belfort; Timothy Bestor; Ashok S Bhagwat; Thomas A Bickle; Jurate Bitinaite; Robert Blumenthal; Sergey Kh Degtyarev; David T F Dryden; Kevin Dybvig; Keith Firman; Elizaveta S Gromova; Richard I Gumport; Stephen E Halford; Stanley Martin Hattman; Joseph Heitman; David P Hornby; Arvydas Janulaitis; Albert Jeltsch; Jytte Josephsen; Antal Kiss; Todd R Klaenhammer; Ichizo Kobayashi; Huimin Kong; Detlev H Krüger; Sanford Lacks; Martin G Marinus; Michiko Miyahara; Richard D Morgan; Noreen E Murray; Valakunja Nagaraja; Andrzej Piekarowicz; Alfred Pingoud; Elisabeth Raleigh; Desirazu N Rao; Norbert Reich; Vladimir Repin; Eric U Selker; Pang-Chui Shaw; Daniel C. Stein; Barry L Stoddard; Waclaw Szybalski; Thomas A Trautner; James L Van Etten; Jorge M B Vitor; Geoffrey G Wilson; Shuang-yong Xu
A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes.
Nucleic acids research 2003;31(7):1805-12.
2003: Jochen Wilhelm; Alfred Pingoud; Meinhard Hahn
SoFAR: software for fully automatic evaluation of real-time PCR data.
BioTechniques 2003;34(2):324-32.
2003: Melanie Königshoff; Jochen Wilhelm; Rainer M Bohle; Alfred Pingoud; Meinhard Hahn
HER-2/neu gene copy number quantified by real-time PCR: comparison of gene amplification, heterozygosity, and immunohistochemical status in breast cancer tissue.
Clinical chemistry 2003;49(2):219-29.
2003: Peter Friedhoff; Evangelos Thomas; Alfred Pingoud
Tyr212: a key residue involved in strand discrimination by the DNA mismatch repair endonuclease MutH.
Journal of molecular biology 2003;325(2):285-97.
2003: Thomas Lanio; Albert Jeltsch; Alfred Pingoud
High-throughput purification of polyHis-tagged recombinant fusion proteins.
Methods in molecular biology (Clifton, N.J.) 2003;205():199-203.
2002: Erik Werner; Wolfgang Wende; Alfred Pingoud; Udo Heinemann
High resolution crystal structure of domain I of the Saccharomyces cerevisiae homing endonuclease PI-SceI.
Nucleic acids research 2002;30(18):3962-71.
2002: Evangelos Thomas; Alfred Pingoud; Peter Friedhoff
An efficient method for the preparation of long heteroduplex DNA as substrate for mismatch repair by the Escherichia coli MutHLS system.
Biological chemistry 2002;383(9):1459-62.
2002: Jochen Wilhelm; Hendrik Reuter; Björn Tews; Alfred Pingoud; Meinhard Hahn
Detection and quantification of insertion/deletion variations by allele-specific real-time PCR: application for genotyping and chimerism analysis.
Biological chemistry 2002;383(9):1423-33.
2002: Thomas W Kirby; Geoffrey A Mueller; Eugene F DeRose; Mark S Lebetkin; Gregor Meiss; Alfred Pingoud; Robert E London
The nuclease A inhibitor represents a new variation of the rare PR-1 fold.
Journal of molecular biology 2002;320(4):771-82.
2002: Sebastian R Scholz; Christian Korn; Oleg Gimadutdinow; Michael Knoblauch; Alfred Pingoud; Gregor Meiss
The effect of ICAD-S on the formation and intracellular distribution of a nucleolytically active caspase-activated DNase.
Nucleic acids research 2002;30(14):3045-51.
2002: Kristin Eisenschmidt; Thomas Lanio; Albert Jeltsch; Alfred Pingoud
A fluorimetric assay for on-line detection of DNA cleavage by restriction endonucleases.
Journal of biotechnology 2002;96(2):185-91.
2002: Uwe Pieper; Detlef H Groll; Sandra Wünsch; Frank-Ulrich Gast; Christian Speck; Norbert Mücke; Alfred Pingoud
The GTP-dependent restriction enzyme McrBC from Escherichia coli forms high-molecular mass complexes with DNA and produces a cleavage pattern with a characteristic 10-base pair repeat.
Biochemistry 2002;41(16):5245-54.
2002: Uwe Pieper; Alfred Pingoud
A mutational analysis of the PD...D/EXK motif suggests that McrC harbors the catalytic center for DNA cleavage by the GTP-dependent restriction enzyme McrBC from Escherichia coli.
Biochemistry 2002;41(16):5236-44.
2002: Vera Pingoud; Elena Kubareva; Gudrun Stengel; Peter Friedhoff; Janusz Bujnicki; Claus Urbanke; Anna Sudina; Alfred Pingoud
Evolutionary relationship between different subgroups of restriction endonucleases.
The Journal of biological chemistry 2002;277(16):14306-14.
2002: Christian Korn; Sebastian R Scholz; Oleg Gimadutdinow; Alfred Pingoud; Gregor Meiss
Involvement of conserved histidine, lysine and tyrosine residues in the mechanism of DNA cleavage by the caspase-3 activated DNase CAD.
Nucleic acids research 2002;30(6):1325-32.
2002: Peter Friedhoff; Babak Sheybani; Evangelos Thomas; Christian Merz; Alfred Pingoud
Haemophilus influenzae and Vibrio cholerae genes for mutH are able to fully complement a mutH defect in Escherichia coli.
FEMS microbiology letters 2002;208(1):123-8.
2001: Gregor Meiss; Sebastian R Scholz; Christian Korn; Oleg Gimadutdinow; Alfred Pingoud
Identification of functionally relevant histidine residues in the apoptotic nuclease CAD.
Nucleic acids research 2001;29(19):3901-9.
2001: Alfred Pingoud; Albert Jeltsch
Structure and function of type II restriction endonucleases.
Nucleic acids research 2001;29(18):3705-27.
2001: M Hahn; J Wilhelm; Alfred Pingoud
Influence of fluorophor dye labels on the migration behavior of polymerase chain reaction--amplified short tandem repeats during denaturing capillary electrophoresis.
Electrophoresis 2001;22(13):2691-700.
2001: Peter Friedhoff; Rudi Lurz; Gerhild Lüder; Alfred Pingoud
Sau3AI, a monomeric type II restriction endonuclease that dimerizes on the DNA and thereby induces DNA loops.
The Journal of biological chemistry 2001;276(26):23581-8.
2001: Frank-Ulrich Gast; Alfred Pingoud
Nucleic acid quantification by delta pH measurement.
Journal of biotechnology 2001;88(2):107-17.
2001: Frank-Ulrich Gast; I Franke; Gregor Meiss; Alfred Pingoud
Immobilization of sugar-non-specific nucleases by utilizing the streptavidin--biotin interaction.
Journal of biotechnology 2001;87(2):131-41.
2001: Jochen Wilhelm; Alfred Pingoud; Meinhard Hahn
Comparison between Taq DNA polymerase and its Stoffel fragment for quantitative real-time PCR with hybridization probes.
BioTechniques 2001;30(5):1052-6, 1058, 1060 passim.
2001: Gregor Meiss; Oleg Gimadutdinow; Peter Friedhoff; Alfred Pingoud
Microtiter-plate assay and related assays for nonspecific endonucleases.
Methods in molecular biology (Clifton, N.J.) 2001;160():37-48.
2001: Albert Jeltsch; Alfred Pingoud
Methods for determining activity and specificity of DNA binding and DNA cleavage by class II restriction endonucleases.
Methods in molecular biology (Clifton, N.J.) 2001;160():287-308.
2000: S Schöttler; Wolfgang Wende; Vera Pingoud; Alfred Pingoud
Identification of Asp218 and Asp326 as the principal Mg2+ binding ligands of the homing endonuclease PI-SceI.
Biochemistry 2000;39(51):15895-900.
2000: Wolfgang Wende; S Schöttler; W Grindl; F Christ; Shawn Steuer; A J Nöel; Vera Pingoud; Alfred Pingoud
[Analysis of binding and cleavage of DNA by the gene conversion PI-SCEI endonuclease using site-directed mutagenesis].
Molekuliarnaia biologiia 2000;34(6):1054-64.
2000: Jochen Wilhelm; Meinhard Hahn; Alfred Pingoud
Influence of DNA target melting behavior on real-time PCR quantification.
Clinical chemistry 2000;46(11):1738-43.
2000: Gregor Meiss; U Böttcher; Christian Korn; Oleg Gimadutdinow; Alfred Pingoud
Vectors for dual expression of target genes in bacterial and mammalian cells.
BioTechniques 2000;29(3):476-8, 480.
2000: Christian Korn; Gregor Meiss; Frank-Ulrich Gast; Oleg Gimadutdinow; Claus Urbanke; Alfred Pingoud
Genetic engineering of Escherichia coli to produce a 1:1 complex of the anabaena sp. PCC 7120 nuclease NucA and its inhibitor NuiA.
Gene 2000;253(2):221-9.
2000: Thomas Lanio; Albert Jeltsch; Alfred Pingoud
Automated purification of His6-tagged proteins allows exhaustive screening of libraries generated by random mutagenesis.
BioTechniques 2000;29(2):338-42.
2000: F Christ; Shawn Steuer; Hubert Thole; Wolfgang Wende; Alfred Pingoud; Vera Pingoud
A model for the PI-SceIxDNA complex based on multiple base and phosphate backbone-specific photocross-links.
Journal of molecular biology 2000;300(4):867-75.
2000: Thomas Lanio; Albert Jeltsch; Alfred Pingoud
On the possibilities and limitations of rational protein design to expand the specificity of restriction enzymes: a case study employing EcoRV as the target.
Protein engineering 2000;13(4):275-81.
2000: Gregor Meiss; Oleg Gimadutdinow; B Haberland; Alfred Pingoud
Mechanism of DNA cleavage by the DNA/RNA-non-specific Anabaena sp. PCC 7120 endonuclease NucA and its inhibition by NuiA.
Journal of molecular biology 2000;297(2):521-34.
2000: Elena Kubareva; Hubert Thole; Anna Karyagina; Tatiana S Oretskaya; Alfred Pingoud; Vera Pingoud
Identification of a base-specific contact between the restriction endonuclease SsoII and its recognition sequence by photocross-linking.
Nucleic acids research 2000;28(5):1085-91.
1999: F Christ; S Schoettler; Wolfgang Wende; Shawn Steuer; Alfred Pingoud; Vera Pingoud
The monomeric homing endonuclease PI-SceI has two catalytic centres for cleavage of the two strands of its DNA substrate.
The EMBO journal 1999;18(24):6908-16.
1999: Uwe Pieper; Thomas Schweitzer; Detlef H Groll; Alfred Pingoud
Defining the location and function of domains of McrB by deletion mutagenesis.
Biological chemistry 1999;380(10):1225-30.
1999: Uwe Pieper; Thomas Schweitzer; Detlef H Groll; Frank-Ulrich Gast; Alfred Pingoud
The GTP-binding domain of McrB: more than just a variation on a common theme?
Journal of molecular biology 1999;292(3):547-56.
1999: T K Srivastava; Peter Friedhoff; Alfred Pingoud; Seturam B Katti
Application of oligonucleoside methylphosphonates in the studies on phosphodiester hydrolysis by Serratia endonuclease.
Nucleosides & nucleotides 1999;18(9):1945-60.
1999: Gregor Meiss; Frank-Ulrich Gast; Alfred Pingoud
The DNA/RNA non-specific Serratia nuclease prefers double-stranded A-form nucleic acids as substrates.
Journal of molecular biology 1999;288(3):377-90.
1999: Vera Pingoud; Hubert Thole; F Christ; W Grindl; Wolfgang Wende; Alfred Pingoud
Photocross-linking of the homing endonuclease PI-SceI to its recognition sequence.
The Journal of biological chemistry 1999;274(15):10235-43.
1999: Giedrius Sasnauskas; Albert Jeltsch; Alfred Pingoud; Virginijus Siksnys
Plasmid DNA cleavage by MunI restriction enzyme: single-turnover and steady-state kinetic analysis.
Biochemistry 1999;38(13):4028-36.
1999: Peter Friedhoff; I Franke; Gregor Meiss; Wolfgang Wende; K L Krause; Alfred Pingoud
A similar active site for non-specific and specific endonucleases.
Nature structural biology 1999;6(2):112-3.
1999: Peter Friedhoff; I Franke; K L Krause; Alfred Pingoud
Cleavage experiments with deoxythymidine 3',5'-bis-(p-nitrophenyl phosphate) suggest that the homing endonuclease I-PpoI follows the same mechanism of phosphodiester bond hydrolysis as the non-specific Serratia nuclease.
FEBS letters 1999;443(2):209-14.
1999: I Franke; Gregor Meiss; Alfred Pingoud
On the advantage of being a dimer, a case study using the dimeric Serratia nuclease and the monomeric nuclease from Anabaena sp. strain PCC 7120.
The Journal of biological chemistry 1999;274(2):825-32.
1999: Meinhard Hahn; Alfred Pingoud
Quantitative PCR with Internal Standardization and OLA-ELISA Product Analysis for the p53 Tumor Suppressor Gene.
Methods in molecular medicine 1999;26():217-31.
1999: I Franke; Alfred Pingoud
Synthesis and biochemical characterization of obligatory dimers of the sugar non-specific nuclease from Serratia marcescens using specifically designed bismaleimidoalkanes as SH-specific crosslinking reagents.
Journal of protein chemistry 1999;18(1):137-46.
1998: C Schulze; Albert Jeltsch; I Franke; Claus Urbanke; Alfred Pingoud
Crosslinking the EcoRV restriction endonuclease across the DNA-binding site reveals transient intermediates and conformational changes of the enzyme during DNA binding and catalytic turnover.
The EMBO journal 1998;17(22):6757-66.
1998: S Schöttler; C Wenz; Thomas Lanio; Albert Jeltsch; Alfred Pingoud
Protein engineering of the restriction endonuclease EcoRV--structure-guided design of enzyme variants that recognize the base pairs flanking the recognition site.
European journal of biochemistry / FEBS 1998;258(1):184-91.
1998: Vera Pingoud; W Grindl; Wolfgang Wende; Hubert Thole; Alfred Pingoud
Structural and functional analysis of the homing endonuclease PI-sceI by limited proteolytic cleavage and molecular cloning of partial digestion products.
Biochemistry 1998;37(22):8233-43.
1998: F Stahl; Wolfgang Wende; C Wenz; Albert Jeltsch; Alfred Pingoud
Intra- vs intersubunit communication in the homodimeric restriction enzyme EcoRV: Thr 37 and Lys 38 involved in indirect readout are only important for the catalytic activity of their own subunit.
Biochemistry 1998;37(16):5682-8.
1998: W Grindl; Wolfgang Wende; Vera Pingoud; Alfred Pingoud
The protein splicing domain of the homing endonuclease PI-sceI is responsible for specific DNA binding.
Nucleic acids research 1998;26(8):1857-62.
1998: I Franke; Gregor Meiss; D Blecher; Oleg Gimadutdinow; Claus Urbanke; Alfred Pingoud
Genetic engineering, production and characterisation of monomeric variants of the dimeric Serratia marcescens endonuclease.
FEBS letters 1998;425(3):517-22.
1998: F Stahl; Wolfgang Wende; Albert Jeltsch; Alfred Pingoud
The mechanism of DNA cleavage by the type II restriction enzyme EcoRV: Asp36 is not directly involved in DNA cleavage but serves to couple indirect readout to catalysis.
Biological chemistry 1998;379(4-5):467-73.
1998: C Wenz; M Hahn; Alfred Pingoud
Engineering of variants of the restriction endonuclease EcoRV that depend in their cleavage activity on the flexibility of sequences flanking the recognition site.
Biochemistry 1998;37(8):2234-42.
1998: Albert Jeltsch; Alfred Pingoud
Kinetic characterization of linear diffusion of the restriction endonuclease EcoRV on DNA.
Biochemistry 1998;37(8):2160-9.
1998: Gregor Meiss; I Franke; Oleg Gimadutdinow; Claus Urbanke; Alfred Pingoud
Biochemical characterization of Anabaena sp. strain PCC 7120 non-specific nuclease NucA and its inhibitor NuiA.
European journal of biochemistry / FEBS 1998;251(3):924-34.
1998: Thomas Lanio; Albert Jeltsch; Alfred Pingoud
Towards the design of rare cutting restriction endonucleases: using directed evolution to generate variants of EcoRV differing in their substrate specificity by two orders of magnitude.
Journal of molecular biology 1998;283(1):59-69.
1997: Detlef H Groll; Albert Jeltsch; U Selent; Alfred Pingoud
Does the restriction endonuclease EcoRV employ a two-metal-Ion mechanism for DNA cleavage?
Biochemistry 1997;36(38):11389-401.
1997: Uwe Pieper; Thomas BRINKMANN; T Krüger; M Noyer-Weidner; Alfred Pingoud
Characterization of the interaction between the restriction endonuclease McrBC from E. coli and its cofactor GTP.
Journal of molecular biology 1997;272(2):190-9.
1997: Frank-Ulrich Gast; T Brinkmann; Uwe Pieper; T Krüger; M Noyer-Weidner; Alfred Pingoud
The recognition of methylated DNA by the GTP-dependent restriction endonuclease McrBC resides in the N-terminal domain of McrB.
Biological chemistry 1997;378(9):975-82.
1997: Alfred Pingoud; Albert Jeltsch
Recognition and cleavage of DNA by type-II restriction endonucleases.
European journal of biochemistry / FEBS 1997;246(1):1-22.
1996: B Kolmes; I Franke; Peter Friedhoff; Alfred Pingoud
Analysis of the reaction mechanism of the non-specific endonuclease of Serratia marcescens using an artificial minimal substrate.
FEBS letters 1996;397(2-3):343-6.
1996: Thomas Lanio; U Selent; C Wenz; Wolfgang Wende; A Schulz; M Adiraj; Seturam B Katti; Alfred Pingoud
EcoRV-T94V: a mutant restriction endonuclease with an altered substrate specificity towards modified oligodeoxynucleotides.
Protein engineering 1996;9(11):1005-10.
1996: Wolfgang Wende; W Grindl; F Christ; Alfred Pingoud; Vera Pingoud
Binding, bending and cleavage of DNA substrates by the homing endonuclease Pl-SceI.
Nucleic acids research 1996;24(21):4123-32.
1996: Peter Friedhoff; Gregor Meiss; B Kolmes; Uwe Pieper; Oleg Gimadutdinow; Claus Urbanke; Alfred Pingoud
Kinetic analysis of the cleavage of natural and synthetic substrates by the Serratia nuclease.
European journal of biochemistry / FEBS 1996;241(2):572-80.
1996: Wolfgang Wende; F Stahl; Alfred Pingoud
The production and characterization of artificial heterodimers of the restriction endonuclease EcoRV.
Biological chemistry 1996;377(10):625-32.
1996: Albert Jeltsch; C Wenz; F Stahl; Alfred Pingoud
Linear diffusion of the restriction endonuclease EcoRV on DNA is essential for the in vivo function of the enzyme.
The EMBO journal 1996;15(18):5104-11.
1996: Peter Friedhoff; S E Matzen; Gregor Meiss; Alfred Pingoud
A quantitative microtiter plate nuclease assay based on ethidium/DNA fluorescence.
Analytical biochemistry 1996;240(2):283-8.
1996: Peter Friedhoff; B Kolmes; Oleg Gimadutdinow; Wolfgang Wende; K L Krause; Alfred Pingoud
Analysis of the mechanism of the Serratia nuclease using site-directed mutagenesis.
Nucleic acids research 1996;24(14):2632-9.
1996: Albert Jeltsch; C Wenz; Wolfgang Wende; U Selent; Alfred Pingoud
Engineering novel restriction endonucleases: principles and applications.
Trends in biotechnology 1996;14(7):235-8.
1996: F Stahl; Wolfgang Wende; Albert Jeltsch; Alfred Pingoud
Introduction of asymmetry in the naturally symmetric restriction endonuclease EcoRV to investigate intersubunit communication in the homodimeric protein.
Proceedings of the National Academy of Sciences of the United States of America 1996;93(12):6175-80.
1996: Albert Jeltsch; T Sobotta; Alfred Pingoud
Structure prediction of the EcoRV DNA methyltransferase based on mutant profiling, secondary structure analysis, comparison with known structures of methyltransferases and isolation of catalytically inactive single mutants.
Protein engineering 1996;9(5):413-23.
1996: C Wenz; Albert Jeltsch; Alfred Pingoud
Probing the indirect readout of the restriction enzyme EcoRV. Mutational analysis of contacts to the DNA backbone.
The Journal of biological chemistry 1996;271(10):5565-73.
1996: Albert Jeltsch; Alfred Pingoud
Horizontal gene transfer contributes to the wide distribution and evolution of type II restriction-modification systems.
Journal of molecular evolution 1996;42(2):91-6.
1995: Gregor Meiss; Peter Friedhoff; M Hahn; Oleg Gimadutdinow; Alfred Pingoud
Sequence preferences in cleavage of dsDNA and ssDNA by the extracellular Serratia marcescens endonuclease.
Biochemistry 1995;34(37):11979-88.
1995: M Hahn; V Dörsam; Peter Friedhoff; A Fritz; Alfred Pingoud
Quantitative polymerase chain reaction with enzyme-linked immunosorbent assay detection of selectively digested amplified sample and control DNA.
Analytical biochemistry 1995;229(2):236-48.
1995: Albert Jeltsch; M Kröger; Alfred Pingoud
Evidence for an evolutionary relationship among type-II restriction endonucleases.
Gene 1995;160(1):7-16.
1995: M Hahn; S E Matzen; Jürgen Serth; Alfred Pingoud
Semiautomated quantitative detection of loss of heterozygosity in the tumor suppressor gene p53.
BioTechniques 1995;18(6):1040-7.
1995: Albert Jeltsch; M Pleckaityte; U Selent; Heiner Wolfes; Virginijus Siksnys; Alfred Pingoud
Evidence for substrate-assisted catalysis in the DNA cleavage of several restriction endonucleases.
Gene 1995;157(1-2):157-62.
1995: Albert Jeltsch; H Maschke; U Selent; C Wenz; E Köhler; Bernard A Connolly; H Thorogood; Alfred Pingoud
DNA binding specificity of the EcoRV restriction endonuclease is increased by Mg2+ binding to a metal ion binding site distinct from the catalytic center of the enzyme.
Biochemistry 1995;34(18):6239-46.
1995: M Hahn; R Fislage; Alfred Pingoud
Polymorphism of the pentanucleotide repeat d(AAAAT) within intron 1 of the human tumor suppressor gene p53 (17p13.1).
Human genetics 1995;95(4):471-2.
1995: Albert Jeltsch; Jürgen Alves; Claus Urbanke; G Maass; H Eckstein; Z Lianshan; E Bayer; Alfred Pingoud
A dodecapeptide comprising the extended chain-alpha 4 region of the restriction endonuclease EcoRI specifically binds to the EcoRI recognition site.
The Journal of biological chemistry 1995;270(10):5122-9.
1994: T Oswald; Wolfgang Wende; Alfred Pingoud; Ursula Rinas
Comparison of N-terminal affinity fusion domains: effect on expression level and product heterogeneity of recombinant restriction endonuclease EcoRV.
Applied microbiology and biotechnology 1994;42(1):73-7.
1994: C Wenz; U Selent; Wolfgang Wende; Albert Jeltsch; Heiner Wolfes; Alfred Pingoud
Protein engineering of the restriction endonuclease EcoRV: replacement of an amino acid residue in the DNA binding site leads to an altered selectivity towards unmodified and modified substrates.
Biochimica et biophysica acta 1994;1219(1):73-80.
1994: Albert Jeltsch; Jürgen Alves; Heiner Wolfes; G Maass; Alfred Pingoud
Pausing of the restriction endonuclease EcoRI during linear diffusion on DNA.
Biochemistry 1994;33(34):10215-9.
1994: Peter Friedhoff; Oleg Gimadutdinow; Alfred Pingoud
Identification of catalytically relevant amino acids of the extracellular Serratia marcescens endonuclease by alignment-guided mutagenesis.
Nucleic acids research 1994;22(16):3280-7.
1994: Peter Friedhoff; Oleg Gimadutdinow; T Rüter; Wolfgang Wende; Claus Urbanke; Hubert Thole; Alfred Pingoud
A procedure for renaturation and purification of the extracellular Serratia marcescens nuclease from genetically engineered Escherichia coli.
Protein expression and purification 1994;5(1):37-43.
1993: Peter Friedhoff; M Hahn; Heiner Wolfes; Alfred Pingoud
Quantitative polymerase chain reaction with oligodeoxynucleotide ligation assay/enzyme-linked immunosorbent assay detection.
Analytical biochemistry 1993;215(1):9-16.
1993: Albert Jeltsch; Jürgen Alves; Heiner Wolfes; G Maass; Alfred Pingoud
Substrate-assisted catalysis in the cleavage of DNA by the EcoRI and EcoRV restriction enzymes.
Proceedings of the National Academy of Sciences of the United States of America 1993;90(18):8499-503.
1993: Albert Jeltsch; A Fritz; Jürgen Alves; Heiner Wolfes; Alfred Pingoud
A fast and accurate enzyme-linked immunosorbent assay for the determination of the DNA cleavage activity of restriction endonucleases.
Analytical biochemistry 1993;213(2):234-40.
1993: Timo Stöver; E Köhler; U Fagin; Wolfgang Wende; Heiner Wolfes; Alfred Pingoud
Determination of the DNA bend angle induced by the restriction endonuclease EcoRV in the presence of Mg2+.
The Journal of biological chemistry 1993;268(12):8645-50.
1993: M Hahn; Jürgen Serth; R Fislage; H Wolfes; E Allhoff; V Jonas; Alfred Pingoud
Polymerase chain reaction detection of a highly polymorphic VNTR segment in intron 1 of the human p53 gene.
Clinical chemistry 1993;39(3):549-50.
1993: Albert Jeltsch; Jürgen Alves; Thomas Oelgeschläger; Heiner Wolfes; G Maass; Alfred Pingoud
Mutational analysis of the function of Gln115 in the EcoRI restriction endonuclease, a critical amino acid for recognition of the inner thymidine residue in the sequence -GAATTC- and for coupling specific DNA binding to catalysis.
Journal of molecular biology 1993;229(1):221-34.
1993: Alfred Pingoud; J Alves; R Geiger
Restriction enzymes.
Methods in molecular biology (Clifton, N.J.) 1993;16():107-200.
1992: Jürgen Serth; W Weber; M Frech; Alfred Wittinghofer; Alfred Pingoud
Binding of the H-ras p21 GTPase activating protein by the activated epidermal growth factor receptor leads to inhibition of the p21 GTPase activity in vitro.
Biochemistry 1992;31(28):6361-5.
1992: Albert Jeltsch; Jürgen Alves; G Maass; Alfred Pingoud
On the catalytic mechanism of EcoRI and EcoRV. A detailed proposal based on biochemical results, structural data and molecular modelling.
FEBS letters 1992;304(1):4-8.
1992: U Selent; T Rüter; E Köhler; M Liedtke; V Thielking; Jürgen Alves; Thomas Oelgeschläger; Heiner Wolfes; F Peters; Alfred Pingoud
A site-directed mutagenesis study to identify amino acid residues involved in the catalytic function of the restriction endonuclease EcoRV.
Biochemistry 1992;31(20):4808-15.
1992: V Thielking; U Selent; E Köhler; A Landgraf; Heiner Wolfes; Jürgen Alves; Alfred Pingoud
Mg2+ confers DNA binding specificity to the EcoRV restriction endonuclease.
Biochemistry 1992;31(15):3727-32.
1991: A Landgraf; B Reckmann; Alfred Pingoud
Direct analysis of polymerase chain reaction products using enzyme-linked immunosorbent assay techniques.
Analytical biochemistry 1991;198(1):86-91.
1991: V Thielking; U Selent; E Köhler; Heiner Wolfes; Uwe Pieper; R Geiger; Claus Urbanke; F K Winkler; Alfred Pingoud
Site-directed mutagenesis studies with EcoRV restriction endonuclease to identify regions involved in recognition and catalysis.
Biochemistry 1991;30(26):6416-22.
1991: K Bensch; Uwe Pieper; G Ott; N Schirmer; M Sprinzl; Alfred Pingoud
How many EF-Tu molecules participate in aminoacyl-tRNA binding?
Biochimie 1991;73(7-8):1045-50.
1991: Jürgen Serth; A Lautwein; M Frech; Alfred Wittinghofer; Alfred Pingoud
The inhibition of the GTPase activating protein-Ha-ras interaction by acidic lipids is due to physical association of the C-terminal domain of the GTPase activating protein with micellar structures.
The EMBO journal 1991;10(6):1325-30.
1991: A Landgraf; B Reckmann; Alfred Pingoud
Quantitative analysis of polymerase chain reaction (PCR) products using primers labeled with biotin and a fluorescent dye.
Analytical biochemistry 1991;193(2):231-5.
1990: Uwe Pieper; H J Ehbrecht; Anja Fliess; B Schick; Frances Jurnak; Alfred Pingoud
Genetic engineering, isolation and characterization of a truncated Escherichia coli elongation factor Tu comprising domains 2 and 3.
Biochimica et biophysica acta 1990;1087(2):147-56.
1990: Alfred Pingoud; Frank-Ulrich Gast; F Peters
The influence of the concentrations of elongation factors and tRNAs on the dynamics and accuracy of protein biosynthesis.
Biochimica et biophysica acta 1990;1050(1-3):252-8.
1990: V Thielking; Jürgen Alves; Anja Fliess; G Maass; Alfred Pingoud
Accuracy of the EcoRI restriction endonuclease: binding and cleavage studies with oligodeoxynucleotide substrates containing degenerate recognition sequences.
Biochemistry 1990;29(19):4682-91.
1990: Thomas Oelgeschläger; R Geiger; T Rüter; Jürgen Alves; Anja Fliess; Alfred Pingoud
Probing the function of individual amino acid residues in the DNA binding site of the EcoRI restriction endonuclease by analysing the toxicity of genetically engineered mutants.
Gene 1990;89(1):19-27.
1989: Jürgen Alves; Claus Urbanke; Anja Fliess; G Maass; Alfred Pingoud
Fluorescence stopped-flow kinetics of the cleavage of synthetic oligodeoxynucleotides by the EcoRI restriction endonuclease.
Biochemistry 1989;28(19):7879-88.
1989: G H Haase; M Brune; Jochen Reinstein; Emil F Pai; Alfred Pingoud; Alfred Wittinghofer
Adenylate kinases from thermosensitive Escherichia coli strains.
Journal of molecular biology 1989;207(1):151-62.
1989: Jürgen Alves; T Rüter; R Geiger; Anja Fliess; G Maass; Alfred Pingoud
Changing the hydrogen-bonding potential in the DNA binding site of EcoRI by site-directed mutagenesis drastically reduces the enzymatic activity, not, however, the preference of this restriction endonuclease for cleavage within the site-GAATTC-.
Biochemistry 1989;28(6):2678-84.
1989: R Geiger; T Rüter; Jürgen Alves; Anja Fliess; Heiner Wolfes; V Pingoud; Claus Urbanke; G Maass; Alfred Pingoud; A Düsterhöft
Genetic engineering of EcoRI mutants with altered amino acid residues in the DNA binding site: physicochemical investigations give evidence for an altered monomer/dimer equilibrium for the Gln144Lys145 and Gln144Lys145Lys200 mutants.
Biochemistry 1989;28(6):2667-77.
1988: Anja Fliess; Heiner Wolfes; F Seela; Alfred Pingoud
Analysis of the recognition mechanism involved in the EcoRV catalyzed cleavage of DNA using modified oligodeoxynucleotides.
Nucleic acids research 1988;16(24):11781-93.
1988: Alfred Pingoud; B M Willumsen; D Gallwitz; S B Masters; J W Hershey
The guanine-nucleotide binding proteins. EMBO-NATO-CEC advanced research workshop organized by L. Bosch, B. Kraal and A. Parmeggiani in Renesse, The Netherlands, August 6-11, 1988.
FEBS letters 1988;242(1):1-3.
1988: Alfred Pingoud; M Wehrmann; Uwe Pieper; Frank-Ulrich Gast; Claus Urbanke; Jürgen Alves; Juergen Feuerstein; Alfred Wittinghofer
Spectroscopic and hydrodynamic studies reveal structural differences in normal and transforming H-ras gene products.
Biochemistry 1988;27(13):4735-40.
1987: Frank-Ulrich Gast; F Peters; Alfred Pingoud
The role of translocation in ribosomal accuracy. Translocation rates for cognate and noncognate aminoacyl- and peptidyl-tRNAs on Escherichia coli ribosomes.
The Journal of biological chemistry 1987;262(25):11920-6.
1986: Heiner Wolfes; Jürgen Alves; Anja Fliess; R Geiger; Alfred Pingoud
Site directed mutagenesis experiments suggest that Glu 111, Glu 144 and Arg 145 are essential for endonucleolytic activity of EcoRI.
Nucleic acids research 1986;14(22):9063-80.
1986: Heiner Wolfes; Anja Fliess; F Winkler; Alfred Pingoud
Cross-linking of bromodeoxyuridine-substituted oligonucleotides to the EcoRI and EcoRV restriction endonucleases.
European journal of biochemistry / FEBS 1986;159(2):267-73.
1986: Anja Fliess; Heiner Wolfes; A Rosenthal; K Schwellnus; H Blöcker; R Frank; Alfred Pingoud
Role of thymidine residues in DNA recognition by the EcoRI and EcoRV restriction endonucleases.
Nucleic acids research 1986;14(8):3463-74.
1986: S Scholtissek; Alfred Pingoud; G Maass; M Zabeau
Polypeptide sequences involved in the cleavage of DNA by the restriction endonuclease EcoRI.
The Journal of biological chemistry 1986;261(5):2228-34.
1985: Heiner Wolfes; Anja Fliess; Alfred Pingoud
A comparison of the structural requirements for DNA cleavage by the isoschizomers HaeIII, BspRI and BsuRI.
European journal of biochemistry / FEBS 1985;150(1):105-10.
1985: T Sam; Alfred Pingoud; L Bosch
Mutant species of EF-Tu, altered at position 375, exhibit a reduced affinity for aminoacylated transfer-RNAs.
FEBS letters 1985;185(1):51-6.
1985: F U Gast; J Langowski; Alfred Pingoud
Product analysis of in vitro ribosomal protein synthesis for the assessment of kinetic parameters.
Analytical biochemistry 1985;147(2):364-8.
1985: H J Ehbrecht; Alfred Pingoud; Claus Urbanke; G Maass; C Gualerzi
Linear diffusion of restriction endonucleases on DNA.
The Journal of biological chemistry 1985;260(10):6160-6.
1985: Alfred Pingoud
Spermidine increases the accuracy of type II restriction endonucleases. Suppression of cleavage at degenerate, non-symmetrical sites.
European journal of biochemistry / FEBS 1985;147(1):105-9.
1984: Alfred Pingoud; Claus Urbanke; J Alves; H J Ehbrecht; M Zabeau; C Gualerzi
Effect of polyamines and basic proteins on cleavage of DNA by restriction endonucleases.
Biochemistry 1984;23(24):5697-703.
1984: B A Connolly; F Eckstein; Alfred Pingoud
The stereochemical course of the restriction endonuclease EcoRI-catalyzed reaction.
The Journal of biological chemistry 1984;259(17):10760-3.
1984: B A Connolly; B V Potter; F Eckstein; Alfred Pingoud; L Grotjahn
Synthesis and characterization of an octanucleotide containing the EcoRI recognition sequence with a phosphorothioate group at the cleavage site.
Biochemistry 1984;23(15):3443-53.
1984: J Alves; Alfred Pingoud; W Haupt; J Langowski; F Peters; G Maass; C Wolff
The influence of sequences adjacent to the recognition site on the cleavage of oligodeoxynucleotides by the EcoRI endonuclease.
European journal of biochemistry / FEBS 1984;140(1):83-92.
1983: Alfred Pingoud; Frank-Ulrich Gast; W Block; F Peters
The elongation factor Tu from Escherichia coli, aminoacyl-tRNA, and guanosine tetraphosphate form a ternary complex which is bound by programmed ribosomes.
The Journal of biological chemistry 1983;258(23):14200-5.
1983: J Langowski; J Alves; Alfred Pingoud; G Maass
Does the specific recognition of DNA by the restriction endonuclease EcoRI involve a linear diffusion step? Investigation of the processivity of the EcoRI endonuclease.
Nucleic acids research 1983;11(2):501-13.
1982: Alfred Pingoud; W Block; A Wittinghofer; H Wolf; E Fischer
The elongation factor Tu binds aminoacyl-tRNA in the presence of GDP.
The Journal of biological chemistry 1982;257(19):11261-7.
1982: J Alves; Alfred Pingoud; J Langowski; Claus Urbanke; G Maass
Two identical subunits of the EcoRI restriction endonuclease Co-operate in the binding and cleavage of the palindromic substrate.
European journal of biochemistry / FEBS 1982;124(1):139-42.
1982: Alfred Pingoud; W Block; C Urbanke; H Wolf
The antibiotics kirromycin and pulvomycin bind to different sites on the elongation factor Tu from Escherichia coli.
European journal of biochemistry / FEBS 1982;123(2):261-5.
1981: M Goppelt; M Grol; Alfred Pingoud; K Schumacher
Interaction of myelin basic protein with mononuclear cells: the primary reaction for the MEM and EMT tests.
British journal of cancer 1981;44(6):838-45.
1981: M Goppelt; J Langowski; Alfred Pingoud; W Haupt; Claus Urbanke; H Mayer; G Maass
The effect of several nucleic acid binding drugs on the cleavage of d(GGAATTCC) and pBR 322 by the Eco RI restriction endonuclease.
Nucleic acids research 1981;9(22):6115-27.
1981: J Langowski; Claus Urbanke; Alfred Pingoud; G Maass
Transient cleavage kinetics of the Eco RI restriction endonuclease measured in a pulsed quench-flow apparatus: enzyme concentration-dependent activity change.
Nucleic acids research 1981;9(14):3483-90.
1981: W Block; Alfred Pingoud
The identification and analysis of nucleotides bound to the elongation factor Tu from Escherichia coli.
Analytical biochemistry 1981;114(1):112-7.
1981: Alfred Pingoud; W Block
The elongation factor Tu . guanosine tetraphosphate complex.
European journal of biochemistry / FEBS 1981;116(3):631-4.
1981: R Römer; W Block; Alfred Pingoud; H Wolf
A 1H NMR study of the Escherichia coli elongation-factor Tu with guanine nucleotides and the antibiotic kirromycin.
FEBS letters 1981;126(2):161-4.
1981: W Block; Alfred Pingoud; M Khan; P Kjellerup
The pharmacokinetics, bioequivalence and bioavailability of different formulations of metoclopramide in man.
Arzneimittel-Forschung 1981;31(6):1041-5.
1980: J Langowski; Alfred Pingoud; M Goppelt; G Maass
Inhibition of Eco RI action by polynucleotides. A characterization of the non-specific binding of the enzyme to DNA.
Nucleic acids research 1980;8(20):4727-36.
1980: Alfred Pingoud; C Urbanke
Aminoacyl transfer ribonucleic acid binding site of the bacterial elongation factor Tu.
Biochemistry 1980;19(10):2108-12.
1980: M Goppelt; Alfred Pingoud; G Maass; H Mayer; H Köster; R Frank
The interaction of the EcoRI restriction endonuclease with its substrate. A physico-chemical study employing natural and synthetic oligonucleotides and polynucleotides.
European journal of biochemistry / FEBS 1980;104(1):101-7.
1979: F Peters; Alfred Pingoud
Numerical analysis of binding studies: a direct procedure avoiding the pitfalls of a Scatchard analysis of equilibrium data for unknown binding models.
International journal of bio-medical computing 1979;10(5):401-15.
1979: Alfred Pingoud; C Urbanke
The determination of binding parameters from protection experiments. A quantitative assay for ternary complex formation of elongation factor Tu, GTP, and aminoacyl-tRNA.
Analytical biochemistry 1979;92(1):123-7.
1978: Alfred Pingoud; F Peters; G Maass
Chemical modification studies on the tyrosyl-tRNA synthetase/tRNA complex are in agreement with an antico-operative binding scheme.
Journal of molecular biology 1978;125(3):397-9.
1978: Alfred Pingoud; C Urbanke; H Wolf; G Maass
The binding of kirromycin to elongation factor Tu. Structural alterations are responsible for the inhibitory action.
European journal of biochemistry / FEBS 1978;86(1):153-7.
1977: Alfred Pingoud; C Urbanke; G Krauss; F Peters; G Maass
Ternary complex formation between elongation factor Tu, GTP and aminoacyl-tRNA: an equilibrium study.
European journal of biochemistry / FEBS 1977;78(2):403-9.
1977: Alfred Pingoud; R Kownatzki; G Maass
Fluoresceinylthiocarbamyl-tRNATyr: a useful derivative of tRNATyr (E.coli) for physicochemical studies.
Nucleic acids research 1977;4(2):327-38.
1976: D Riesner; Alfred Pingoud; D Boehme; F Peters; G Maass
Distinct steps in the specific binding of tRNA to aminoacyl-tRNA synthetase. Temperature-jump studies on the serine-specific system from yeast and the tyrosine-specific system from Escherichia coli.
European journal of biochemistry / FEBS 1976;68(1):71-80.
1975: G Krauss; Alfred Pingoud; D Boehme; D Riesner; F Peters; G Maas
Equivalent and non-equivalent binding sites for tRNA on aminoacyl-tRNA synthetases.
European journal of biochemistry / FEBS 1975;55(3):517-29.