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Roland Siezen

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Genes & Molecular Sequences

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TOP 3
Lex Overmars; Roland Siezen; Robert Kerkhoven; Christof Francke
MGcV: the microbial genomic context viewer for comparative genome analysis.
Eline H Huisjes; Michiel Kleerebezem; Marijke A H Luttik; Marinka J H Almering; Markus M M Bisschops; Dieu H N Dang; Roland Siezen; Antonius J A van Maris; Jack T. Pronk
Toward pectin fermentation by Saccharomyces cerevisiae: expression of the first two steps of a bacterial pathway for D-galacturonate metabolism.
Yu Zhao; Roland Siezen; Tjakko Abee; Martien P M Caspers; Remco Kort
Complete genome sequence of Geobacillus thermoglucosidans TNO-09.020, a thermophilic sporeformer associated with a dairy-processing environment.

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All Publications

2013: Lex Overmars; Roland Siezen; Robert Kerkhoven; Christof Francke
MGcV: the microbial genomic context viewer for comparative genome analysis.
BMC genomics 2013;14():209.
2012: Eline H Huisjes; Michiel Kleerebezem; Marijke A H Luttik; Marinka J H Almering; Markus M M Bisschops; Dieu H N Dang; Roland Siezen; Antonius J A van Maris; Jack T. Pronk
Toward pectin fermentation by Saccharomyces cerevisiae: expression of the first two steps of a bacterial pathway for D-galacturonate metabolism.
Journal of biotechnology 2012;162(2-3):303-10.
2012: Yu Zhao; Roland Siezen; Tjakko Abee; Martien P M Caspers; Remco Kort
Complete genome sequence of Geobacillus thermoglucosidans TNO-09.020, a thermophilic sporeformer associated with a dairy-processing environment.
Journal of bacteriology 2012;194(15):4118.
2012: Mengjin Liu; Arjen Nauta; Celine Prakash; Roland Siezen; Christof Francke
Computational analysis of cysteine and methionine metabolism and its regulation in dairy starter and related bacteria.
Journal of bacteriology 2012;194(13):3522-33.
2012: Lars Axelsson; Hans Blom; Jos Boekhorst; Michiel Kleerebezem; Kristine Naterstad; Bernadet Renckens; Ida Rud; Sacha A F T van Hijum; Roland Siezen
Genome sequence of the naturally plasmid-free Lactobacillus plantarum strain NC8 (CCUG 61730).
Journal of bacteriology 2012;194(9):2391-2.
2012: Tom Groot Kormelink; Yanick Hagemeijer; Eric Koenders; Lex Overmars; Roland Siezen; Willem M de Vos; Christof Francke
Comparative genome analysis of central nitrogen metabolism and its control by GlnR in the class Bacilli.
BMC genomics 2012;13():191.
2012: Jumamurat R Bayjanov; Douwe Molenaar; Roland Siezen; Vesela A Tzeneva; Sacha A F T van Hijum
PhenoLink--a web-tool for linking phenotype to ~omics data for bacteria: application to gene-trait matching for Lactobacillus plantarum strains.
BMC genomics 2012;13():170.
2011: Jos Boekhorst; Greer Wilson; Roland Siezen
Searching in microbial genomes for encoded small proteins.
Microbial biotechnology 2011;4(3):308-13.
2011: Michiel Wels; Lex Overmars; Christof Francke; Michiel Kleerebezem; Roland Siezen
Reconstruction of the regulatory network of Lactobacillus plantarum WCFS1 on basis of correlated gene expression and conserved regulatory motifs.
Microbial biotechnology 2011;4(3):333-44.
2011: Anita C Schürch; Kristin Kremer; Albert Kiers; Martin J Boeree; Roland Siezen; Dick van Soolingen
Preferential deletion events in the direct repeat locus of Mycobacterium tuberculosis.
Journal of clinical microbiology 2011;49(4):1318-22.
2011: Anita C Schürch; Kristin Kremer; Robin M Warren; Nguyen V Hung; Yanlin Zhao; Kanglin Wan; Martin J Boeree; Roland Siezen; Noel H Smith; Dick Van Soolingen
Mutations in the regulatory network underlie the recent clonal expansion of a dominant subclone of the Mycobacterium tuberculosis Beijing genotype.
Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases 2011;11(3):587-97.
2011: Christof Francke; Tom Groot Kormelink; Yanick Hagemeijer; Roy Moezelaar; Lex Overmars; Vincent Sluijter; Roland Siezen
Comparative analyses imply that the enigmatic Sigma factor 54 is a central controller of the bacterial exterior.
BMC genomics 2011;12():385.
2011: Ronnie Machielsen; Roland Siezen; Sacha A F T van Hijum; Johan E T van Hylckama Vlieg
Molecular description and industrial potential of Tn6098 conjugative transfer conferring alpha-galactoside metabolism in Lactococcus lactis.
Applied and environmental microbiology 2011;77(2):555-63.
2011: Mark de Been; Christof Francke; Roland Siezen; Tjakko Abee
Novel sigmaB regulation modules of Gram-positive bacteria involve the use of complex hybrid histidine kinases.
Microbiology (Reading, England) 2011;157(Pt 1):3-12.
2010: Anita C Schürch; Kristin Kremer; Olaf Daviena; Albert Kiers; Martin J Boeree; Roland Siezen; Dick Van Soolingen
High-resolution typing by integration of genome sequencing data in a large tuberculosis cluster.
Journal of clinical microbiology 2010;48(9):3403-6.
2010: Roland Siezen; Jumamurat Bayjanov; Bernadet Renckens; Michiel Wels; Sacha A F T van Hijum; Douwe Molenaar; Johan E T van Hylckama Vlieg
Complete genome sequence of Lactococcus lactis subsp. lactis KF147, a plant-associated lactic acid bacterium.
Journal of bacteriology 2010;192(10):2649-50.
2010: Jumamurat R Bayjanov; Roland Siezen; Sacha A F T van Hijum
PanCGHweb: a web tool for genotype calling in pangenome CGH data.
Bioinformatics (Oxford, England) 2010;26(9):1256-7.
2010: Michiel Kleerebezem; Pascal Hols; Elvis Bernard; Thomas Jules Rolain; Miaomiao Zhou; Roland Siezen; Peter A Bron
The extracellular biology of the lactobacilli.
FEMS microbiology reviews 2010;34(2):199-230.
2010: Roland Siezen; Vesela A Tzeneva; Anna Castioni; Michiel Wels; Hoa T K Phan; Jan Rademaker; Marjo J C Starrenburg; Michiel Kleerebezem; Douwe Molenaar; Johan E T van Hylckama Vlieg
Phenotypic and genomic diversity of Lactobacillus plantarum strains isolated from various environmental niches.
Environmental microbiology 2010;12(3):758-73.
2010: Mark de Been; Marcel H Tempelaars; Willem van Schaik; Roy Moezelaar; Roland Siezen; Tjakko Abee
A novel hybrid kinase is essential for regulating the sigma(B)-mediated stress response of Bacillus cereus.
Environmental microbiology 2010;12(3):730-45.
2010: Miaomiao Zhou; Daniel Theunissen; Michiel Wels; Roland Siezen
LAB-Secretome: a genome-scale comparative analysis of the predicted extracellular and surface-associated proteins of Lactic Acid Bacteria.
BMC genomics 2010;11():651.
2010: Marnix H Medema; Miaomiao Zhou; Sacha A F T van Hijum; Jolein Gloerich; Hans J C T Wessels; Roland Siezen; Marc Strous
A predicted physicochemically distinct sub-proteome associated with the intracellular organelle of the anammox bacterium Kuenenia stuttgartiensis.
BMC genomics 2010;11():299.
2010: Mengjin Liu; Jumamurat R Bayjanov; Bernadet Renckens; Arjen Nauta; Roland Siezen
The proteolytic system of lactic acid bacteria revisited: a genomic comparison.
BMC genomics 2010;11():36.
2010: Anita C Schürch; Kristin Kremer; Albert Kiers; Olaf Daviena; Martin J Boeree; Roland Siezen; Noel H Smith; Dick Van Soolingen
The tempo and mode of molecular evolution of Mycobacterium tuberculosis at patient-to-patient scale.
Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases 2010;10(1):108-14.
2009: Mengjin Liu; Roland Siezen; Arjen Nauta
In silico prediction of horizontal gene transfer events in Lactobacillus bulgaricus and Streptococcus thermophilus reveals protocooperation in yogurt manufacturing.
Applied and environmental microbiology 2009;75(12):4120-9.
2009: Michiel Wels; Roger S Bongers; Jos Boekhorst; Douwe Molenaar; Mark H.J. Sturme; WILLEM DE VOS; Roland Siezen; Michiel Kleerebezem
Large intergenic cruciform-like supermotifs in the Lactobacillus plantarum genome.
Journal of bacteriology 2009;191(10):3420-3.
2009: Ashok Kumar Patel; Niels van Oosterwijk; Vijay Kumar Singh; Henriëtte J Rozeboom; Kor H Kalk; Roland Siezen; Medicherla V Jagannadham; Bauke W Dijkstra
Crystallization and preliminary X-ray analysis of carnein, a serine protease from Ipomoea carnea.
Acta crystallographica. Section F, Structural biology and crystallization communications 2009;65(Pt 4):383-5.
2009: Jumamurat R Bayjanov; Michiel Wels; Marjo Starrenburg; Johan E T van Hylckama Vlieg; Roland Siezen; Douwe Molenaar
PanCGH: a genotype-calling algorithm for pangenome CGH data.
Bioinformatics (Oxford, England) 2009;25(3):309-14.
2008: Mark de Been; Marieke J Bart; Tjakko Abee; Roland Siezen; Christof Francke
The identification of response regulator-specific binding sites reveals new roles of two-component systems in Bacillus cereus and closely related low-GC Gram-positives.
Environmental microbiology 2008;10(10):2796-809.
2008: Jolanda M Lambert; Roland Siezen; WILLEM DE VOS; Michiel Kleerebezem
Improved annotation of conjugated bile acid hydrolase superfamily members in Gram-positive bacteria.
Microbiology (Reading, England) 2008;154(Pt 8):2492-500.
2008: Mengjin Liu; Arjen Nauta; Christof Francke; Roland Siezen
Comparative genomics of enzymes in flavor-forming pathways from amino acids in lactic acid bacteria.
Applied and environmental microbiology 2008;74(15):4590-600.
2008: Nobuhiro Iritani; Harry Vennema; J Joukje Siebenga; Roland Siezen; Bernadet Renckens; Yoshiyuki Seto; Atsushi Kaida; Marion Koopmans
Genetic analysis of the capsid gene of genotype GII.2 noroviruses.
Journal of virology 2008;82(15):7336-45.
2008: Michiel Wels; Tom Groot Kormelink; Michiel Kleerebezem; Roland Siezen; Christof Francke
An in silico analysis of T-box regulated genes and T-box evolution in prokaryotes, with emphasis on prediction of substrate specificity of transporters.
BMC genomics 2008;9():330.
2008: Miaomiao Zhou; Jos Boekhorst; Christof Francke; Roland Siezen
LocateP: genome-scale subcellular-location predictor for bacterial proteins.
BMC bioinformatics 2008;9():173.
2008: Christof Francke; Robert Kerkhoven; Michiel Wels; Roland Siezen
A generic approach to identify Transcription Factor-specific operator motifs; Inferences for LacI-family mediated regulation in Lactobacillus plantarum WCFS1.
BMC genomics 2008;9():145.
2008: Richard A Notebaart; Bas Teusink; Roland Siezen; Balázs Papp
Co-regulation of metabolic genes is better explained by flux coupling than by network distance.
PLoS computational biology 2008;4(1):e26.
2008: Peter van Baarlen; H Peter van Esse; Roland Siezen; Bart P H J Thomma
Challenges in plant cellular pathway reconstruction based on gene expression profiling.
Trends in plant science 2008;13(1):44-50.
2008: Roland Siezen; Marjo Starrenburg; Jos Boekhorst; Bernadet Renckens; Douwe Molenaar; Johan E T van Hylckama Vlieg
Genome-scale genotype-phenotype matching of two Lactococcus lactis isolates from plants identifies mechanisms of adaptation to the plant niche.
Applied and environmental microbiology 2008;74(2):424-36.
2007: Cindy van der Meer-van Kraaij; Roland Siezen; Evelien Kramer; Marjolein Reinders; Hans Blokzijl; Roelof van der Meer; Jaap Keijer
Dietary modulation and structure prediction of rat mucosal pentraxin (Mptx) protein and loss of function in humans.
Genes & nutrition 2007;2(3):275-85.
2007: Mark H.J. Sturme; Christof Francke; Roland Siezen; WILLEM DE VOS; Michiel Kleerebezem
Making sense of quorum sensing in lactobacilli: a special focus on Lactobacillus plantarum WCFS1.
Microbiology (Reading, England) 2007;153(Pt 12):3939-47.
2007: J Joukje Siebenga; Harry Vennema; Bernadet Renckens; Erwin De Bruin; Bas van der Veer; Roland Siezen; Marion Koopmans
Epochal evolution of GGII.4 norovirus capsid proteins from 1995 to 2006.
Journal of virology 2007;81(18):9932-41.
2007: Roland Siezen; Bernadet Renckens; Jos Boekhorst
Evolution of prokaryotic subtilases: genome-wide analysis reveals novel subfamilies with different catalytic residues.
Proteins 2007;67(3):681-94.
2006: Bas Teusink; Anne Wiersma; Douwe Molenaar; Christof Francke; WILLEM DE VOS; Roland Siezen; Eddy J. Smid
Analysis of growth of Lactobacillus plantarum WCFS1 on a complex medium using a genome-scale metabolic model.
The Journal of biological chemistry 2006;281(52):40041-8.
2006: Jos Boekhorst; Michiel Wels; Michiel Kleerebezem; Roland Siezen
The predicted secretome of Lactobacillus plantarum WCFS1 sheds light on interactions with its environment.
Microbiology (Reading, England) 2006;152(Pt 11):3175-83.
2006: Mark de Been; Christof Francke; Roy Moezelaar; Tjakko Abee; Roland Siezen
Comparative analysis of two-component signal transduction systems of Bacillus cereus, Bacillus thuringiensis and Bacillus anthracis.
Microbiology (Reading, England) 2006;152(Pt 10):3035-48.
2006: Maaike C de Vries; Roland Siezen; Janneke G E Wijman; Yuan Zhao; Michiel Kleerebezem; WILLEM DE VOS; Elaine E Vaughan
Comparative and functional analysis of the rRNA-operons and their tRNA gene complement in different lactic acid bacteria.
Systematic and applied microbiology 2006;29(5):358-67.
2006: Johan E T van Hylckama Vlieg; Jan Rademaker; Herwig Bachmann; Douwe Molenaar; William J Kelly; Roland Siezen
Natural diversity and adaptive responses of Lactococcus lactis.
Current opinion in biotechnology 2006;17(2):183-90.
2006: Richard A Notebaart; Frank H J van Enckevort; Christof Francke; Roland Siezen; Bas Teusink
Accelerating the reconstruction of genome-scale metabolic networks.
BMC bioinformatics 2006;7():296.
2006: Roland Siezen; Jos Boekhorst; Lidia Muscariello; Douwe Molenaar; Bernadet Renckens; Michiel Kleerebezem
Lactobacillus plantarum gene clusters encoding putative cell-surface protein complexes for carbohydrate utilization are conserved in specific gram-positive bacteria.
BMC genomics 2006;7():126.
2006: Michiel Wels; Christof Francke; Robert Kerkhoven; Michiel Kleerebezem; Roland Siezen
Predicting cis-acting elements of Lactobacillus plantarum by comparative genomics with different taxonomic subgroups.
Nucleic acids research 2006;34(7):1947-58.
2006: Jos Boekhorst; Quinta Helmer; Michiel Kleerebezem; Roland Siezen
Comparative analysis of proteins with a mucus-binding domain found exclusively in lactic acid bacteria.
Microbiology (Reading, England) 2006;152(Pt 1):273-80.
2005: Mengjin Liu; Frank H J van Enckevort; Roland Siezen
Genome update: lactic acid bacteria genome sequencing is booming.
Microbiology (Reading, England) 2005;151(Pt 12):3811-4.
2005: Roland Siezen; Bernadet Renckens; Iris I Van Swam; Sander Peters; Richard van Kranenburg; Michiel Kleerebezem; WILLEM DE VOS
Complete sequences of four plasmids of Lactococcus lactis subsp. cremoris SK11 reveal extensive adaptation to the dairy environment.
Applied and environmental microbiology 2005;71(12):8371-82.
2005: Bas Teusink; Frank H J van Enckevort; Christof Francke; Anne Wiersma; Arno Wegkamp; Eddy J. Smid; Roland Siezen
In silico reconstruction of the metabolic pathways of Lactobacillus plantarum: comparing predictions of nutrient requirements with those from growth experiments.
Applied and environmental microbiology 2005;71(11):7253-62.
2005: Christof Francke; Roland Siezen; Bas Teusink
Reconstructing the metabolic network of a bacterium from its genome.
Trends in microbiology 2005;13(11):550-8.
2005: Douwe Molenaar; Françoise Bringel; Frank H J Schuren; WILLEM DE VOS; Roland Siezen; Michiel Kleerebezem
Exploring Lactobacillus plantarum genome diversity by using microarrays.
Journal of bacteriology 2005;187(17):6119-27.
2005: Jos Boekhorst; Mark de Been; Michiel Kleerebezem; Roland Siezen
Genome-wide detection and analysis of cell wall-bound proteins with LPxTG-like sorting motifs.
Journal of bacteriology 2005;187(14):4928-34.
2005: Richard van Kranenburg; Natasa Golic; Roger S Bongers; Rob J Leer; WILLEM DE VOS; Roland Siezen; Michiel Kleerebezem
Functional analysis of three plasmids from Lactobacillus plantarum.
Applied and environmental microbiology 2005;71(3):1223-30.
2005: Richard A Notebaart; Martijn A Huynen; Bas Teusink; Roland Siezen; Berend Snel
Correlation between sequence conservation and the genomic context after gene duplication.
Nucleic acids research 2005;33(19):6164-71.
2005: Eddy J. Smid; F J H van Enckevort; Arno Wegkamp; Jos Boekhorst; Douwe Molenaar; Jeroen Hugenholtz; Roland Siezen; Bas Teusink
Metabolic models for rational improvement of lactic acid bacteria as cell factories.
Journal of applied microbiology 2005;98(6):1326-31.
2004: Tjakko Abee; Willem van Schaik; Roland Siezen
Impact of genomics on microbial food safety.
Trends in biotechnology 2004;22(12):653-60.
2004: Jos Boekhorst; Roland Siezen; Marie-Camille Zwahlen; David Vilanova; R David Pridmore; Annick Mercenier; Michiel Kleerebezem; WILLEM DE VOS; Harald Brüssow; Frank Desiere
The complete genomes of Lactobacillus plantarum and Lactobacillus johnsonii reveal extensive differences in chromosome organization and gene content.
Microbiology (Reading, England) 2004;150(Pt 11):3601-11.
2004: Robert Kerkhoven; Frank H J van Enckevort; Jos Boekhorst; Douwe Molenaar; Roland Siezen
Visualization for genomics: the Microbial Genome Viewer.
Bioinformatics (Oxford, England) 2004;20(11):1812-4.
2004: Roland Siezen; Frank H J van Enckevort; Michiel Kleerebezem; Bas Teusink
Genome data mining of lactic acid bacteria: the impact of bioinformatics.
Current opinion in biotechnology 2004;15(2):105-15.
2003: Marco Ventura; Carlos Canchaya; Michiel Kleerebezem; WILLEM DE VOS; Roland Siezen; Harald Brüssow
The prophage sequences of Lactobacillus plantarum strain WCFS1.
Virology 2003;316(2):245-55.
2003: Michiel Kleerebezem; Jos Boekhorst; Richard van Kranenburg; Douwe Molenaar; Oscar P Kuipers; Rob Leer; Renato Tarchini; Sander A Peters; Hans M Sandbrink; Mark W E J Fiers; Willem J Stiekema; René M Klein Lankhorst; Peter A Bron; Sally M Hoffer; Masja Nierop Groot; Robert Kerkhoven; Maaike de Vries; Björn Ursing; WILLEM DE VOS; Roland Siezen
Complete genome sequence of Lactobacillus plantarum WCFS1.
Proceedings of the National Academy of Sciences of the United States of America 2003;100(4):1990-5.
2002: Todd Klaenhammer; Eric Altermann; Fabrizio Arigoni; Alexander Bolotin; Fred Breidt; Jeffrey Broadbent; Raul Cano; Stephane Chaillou; Josef Deutscher; Mike Gasson; Maarten van de Guchte; Jean Guzzo; Axel Hartke; Trevor Hawkins; Pascal Hols; Robert Hutkins; Michiel Kleerebezem; Jan Kok; Oscar Kuipers; Mark Lubbers; Emmanuelle Maguin; Larry McKay; david mills; Arjen Nauta; Ross Overbeek; Herman Pel; David Pridmore; Milton Saier; Douwe van Sinderen; Alexei Sorokin; James Steele; Daniel O'Sullivan; WILLEM DE VOS; Bart Weimer; Monique Zagorec; Roland Siezen
Discovering lactic acid bacteria by genomics.
Antonie van Leeuwenhoek 2002;82(1-4):29-58.
2002: Roland Siezen; J Kok; T Abee; G Schaafsma
Lactic acid bacteria: genetics, metabolism and applications.
Antonie van Leeuwenhoek 2002;82(1-4):1.
2002: Leon D Kluskens; Wilfried G B Voorhorst; Roland Siezen; Ruth M Schwerdtfeger; Garabed Antranikian; John van der Oost; WILLEM DE VOS
Molecular characterization of fervidolysin, a subtilisin-like serine protease from the thermophilic bacterium Fervidobacterium pennivorans.
Extremophiles : life under extreme conditions 2002;6(3):185-94.
2002: Björn M Ursing; Frank H J van Enckevort; Jack Leunissen; Roland Siezen
EXProt--a database for EXPerimentally verified Protein functions.
In silico biology 2002;2(1):1-4.
2002: Björn M Ursing; Frank H J van Enckevort; Jack Leunissen; Roland Siezen
EXProt: a database for proteins with an experimentally verified function.
Nucleic acids research 2002;30(1):50-1.
2002: María Fernández; Michiel Kleerebezem; Oscar P Kuipers; Roland Siezen; Richard van Kranenburg
Regulation of the metC-cysK operon, involved in sulfur metabolism in Lactococcus lactis.
Journal of bacteriology 2002;184(1):82-90.
2001: Nicolaas C Gey van Pittius; Junaid Gamieldien; Winston Hide; Gordon D Brown; Roland Siezen; Albert D Beyers
The ESAT-6 gene cluster of Mycobacterium tuberculosis and other high G+C Gram-positive bacteria.
Genome biology 2001;2(10):RESEARCH0044.
2001: WILLEM DE VOS; Wilfried G B Voorhorst; M Dijkgraaf; Leon D Kluskens; John van der Oost; Roland Siezen
Purification, characterization, and molecular modeling of pyrolysin and other extracellular thermostable serine proteases from hyperthermophilic microorganisms.
Methods in enzymology 2001;330():383-93.
2000: P G Bruinenberg; WILLEM DE VOS; Roland Siezen
Deletion of various carboxy-terminal domains of Lactococcus lactis SK11 proteinase: effects on activity, specificity, and stability of the truncated enzyme.
Applied and environmental microbiology 2000;66(7):2859-65.
2000: L Fernández; M M Beerthuyzen; J Brown; Roland Siezen; T Coolbear; R Holland; O P Kuipers
Cloning, characterization, controlled overexpression, and inactivation of the major tributyrin esterase gene of Lactococcus lactis.
Applied and environmental microbiology 2000;66(4):1360-8.
1999: C van Kraaij; WILLEM DE VOS; Roland Siezen; Oscar P Kuipers
Lantibiotics: biosynthesis, mode of action and applications.
Natural product reports 1999;16(5):575-87.
1999: Roland Siezen
Multi-domain, cell-envelope proteinases of lactic acid bacteria.
Antonie van Leeuwenhoek 1999;76(1-4):139-55.
1998: C van Kraaij; Eefjan Breukink; M A Noordermeer; Rudy A Demel; Roland Siezen; Oscar P Kuipers; Ben de Kruijff
Pore formation by nisin involves translocation of its C-terminal part across the membrane.
Biochemistry 1998;37(46):16033-40.
1998: Eefjan Breukink; C van Kraaij; Annemieke van Dalen; Rudy A Demel; Roland Siezen; Ben de Kruijff; Oscar P Kuipers
The orientation of nisin in membranes.
Biochemistry 1998;37(22):8153-62.
1997: H W van den Hooven; HS Rollema; Roland Siezen; C W Hilbers; Oscar P Kuipers
Structural features of the final intermediate in the biosynthesis of the lantibiotic nisin. Influence of the leader peptide.
Biochemistry 1997;36(46):14137-45.
1997: Wilfried G B Voorhorst; A Warner; WILLEM DE VOS; Roland Siezen
Homology modelling of two subtilisin-like proteases from the hyperthermophilic archaea Pyrococcus furiosus and Thermococcus stetteri.
Protein engineering 1997;10(8):905-14.
1997: C van Kraaij; Eefjan Breukink; HS Rollema; Roland Siezen; Rudy A Demel; Ben de Kruijff; Oscar P Kuipers
Influence of charge differences in the C-terminal part of nisin on antimicrobial activity and signaling capacity.
European journal of biochemistry / FEBS 1997;247(1):114-20.
1997: Eefjan Breukink; C van Kraaij; Rudy A Demel; Roland Siezen; Oscar P Kuipers; Ben de Kruijff
The C-terminal region of nisin is responsible for the initial interaction of nisin with the target membrane.
Biochemistry 1997;36(23):6968-76.
1997: Roland Siezen; Jack Leunissen
Subtilases: the superfamily of subtilisin-like serine proteases.
Protein science : a publication of the Protein Society 1997;6(3):501-23.
1996: H S Rollema; J W Metzger; P Both; Oscar P Kuipers; Roland Siezen
Structure and biological activity of chemically modified nisin A species.
European journal of biochemistry / FEBS 1996;241(3):716-22.
1996: Mary C Booth; C P Bogie; H G Sahl; Roland Siezen; Kenneth L Hatter; Michael S Gilmore
Structural analysis and proteolytic activation of Enterococcus faecalis cytolysin, a novel lantibiotic.
Molecular microbiology 1996;21(6):1175-84.
1996: Wilfried G B Voorhorst; R I Eggen; A C Geerling; C Platteeuw; Roland Siezen; W M Vos
Isolation and characterization of the hyperthermostable serine protease, pyrolysin, and its gene from the hyperthermophilic archaeon Pyrococcus furiosus.
The Journal of biological chemistry 1996;271(34):20426-31.
1996: H W van den Hooven; F M Lagerwerf; W Heerma; Johan Haverkamp; J C Piard; Cornelis W Hilbers; Roland Siezen; Oscar P Kuipers; H S Rollema
The structure of the lantibiotic lacticin 481 produced by Lactococcus lactis: location of the thioether bridges.
FEBS letters 1996;391(3):317-22.
1996: Roland Siezen; O P Kuipers; WILLEM DE VOS
Comparison of lantibiotic gene clusters and encoded proteins.
Antonie van Leeuwenhoek 1996;69(2):171-84.
1996: Oscar P Kuipers; Gabriele Bierbaum; B Ottenwälder; Helen M Dodd; Nikki Horn; J Metzger; Thomas Kupke; V Gnau; R Bongers; P van den Bogaard; H Kosters; H S Rollema; WILLEM DE VOS; Roland Siezen; G Jung; Friedrich Götz; Hans-Georg Sahl; Michael J Gasson
Protein engineering of lantibiotics.
Antonie van Leeuwenhoek 1996;69(2):161-69.
1996: Rudy A Demel; T Peelen; Roland Siezen; Ben de Kruijff; Oscar P Kuipers
Nisin Z, mutant nisin Z and lacticin 481 interactions with anionic lipids correlate with antimicrobial activity. A monolayer study.
European journal of biochemistry / FEBS 1996;235(1-2):267-74.
1996: Roland Siezen
Subtilases: subtilisin-like serine proteases.
Advances in experimental medicine and biology 1996;379():75-93.
1996: Roland Siezen
Modelling and engineering of enzyme/substrate interactions in subtilisin-like enzymes of unknown 3-dimensional structure.
Advances in experimental medicine and biology 1996;379():63-73.
1995: WILLEM DE VOS; Oscar P Kuipers; J R van der Meer; Roland Siezen
Maturation pathway of nisin and other lantibiotics: post-translationally modified antimicrobial peptides exported by gram-positive bacteria.
Molecular microbiology 1995;17(3):427-37.
1995: H S Rollema; Oscar P Kuipers; P Both; WILLEM DE VOS; Roland Siezen
Improvement of solubility and stability of the antimicrobial peptide nisin by protein engineering.
Applied and environmental microbiology 1995;61(8):2873-8.
1995: M van de Kamp; H W van den Hooven; RN Konings; Gabriele Bierbaum; Hans-Georg Sahl; Oscar P Kuipers; Roland Siezen; WILLEM DE VOS; Cornelis W Hilbers; F J van de Ven
Elucidation of the primary structure of the lantibiotic epilancin K7 from Staphylococcus epidermidis K7. Cloning and characterisation of the epilancin-K7-encoding gene and NMR analysis of mature epilancin K7.
European journal of biochemistry / FEBS 1995;230(2):587-600.
1995: Roland Siezen; H S Rollema; Oscar P Kuipers; WILLEM DE VOS
Homology modelling of the Lactococcus lactis leader peptidase NisP and its interaction with the precursor of the lantibiotic nisin.
Protein engineering 1995;8(2):117-25.
1995: Oscar P Kuipers; H S Rollema; Roland Siezen; WILLEM DE VOS
Lactococcal expression systems for protein engineering of nisin.
Developments in biological standardization 1995;85():605-13.
1994: P G Bruinenberg; WILLEM DE VOS; Roland Siezen
Prevention of C-terminal autoprocessing of Lactococcus lactis SK11 cell-envelope proteinase by engineering of an essential surface loop.
The Biochemical journal 1994;302 ( Pt 3)():957-63.
1994: P G Bruinenberg; P Doesburg; AC Alting; F A Exterkate; WILLEM DE VOS; Roland Siezen
Evidence for a large dispensable segment in the subtilisin-like catalytic domain of the Lactococcus lactis cell-envelope proteinase.
Protein engineering 1994;7(8):991-6.
1994: Roland Siezen; John W M Creemers; W J Van de Ven
Homology modelling of the catalytic domain of human furin. A model for the eukaryotic subtilisin-like proprotein convertases.
European journal of biochemistry / FEBS 1994;222(2):255-66.
1994: J R van der Meer; H S Rollema; Roland Siezen; M M Beerthuyzen; Oscar P Kuipers; WILLEM DE VOS
Influence of amino acid substitutions in the nisin leader peptide on biosynthesis and secretion of nisin by Lactococcus lactis.
The Journal of biological chemistry 1994;269(5):3555-62.
1993: Roland Siezen; P G Bruinenberg; P Vos; I van Alen-Boerrigter; Monique Nijhuis; AC Alting; F A Exterkate; WILLEM DE VOS
Engineering of the substrate-binding region of the subtilisin-like, cell-envelope proteinase of Lactococcus lactis.
Protein engineering 1993;6(8):927-37.
1993: John W M Creemers; Roland Siezen; Anton J M Roebroek; Torik A Y Ayoubi; Danny Huylebroeck; Wim J M Van De Ven
Modulation of furin-mediated proprotein processing activity by site-directed mutagenesis.
The Journal of biological chemistry 1993;268(29):21826-34.
1993: Oscar P Kuipers; H S Rollema; WILLEM DE VOS; Roland Siezen
Biosynthesis and secretion of a precursor of nisin Z by Lactococcus lactis, directed by the leader peptide of the homologous lantibiotic subtilin from Bacillus subtilis.
FEBS letters 1993;330(1):23-7.
1993: Guus Simons; W van den Heuvel; T Reynen; A Frijters; G Rutten; C J Slangen; M Groenen; WILLEM DE VOS; Roland Siezen
Overproduction of bovine beta-casein in Escherichia coli and engineering of its main chymosin cleavage site.
Protein engineering 1993;6(7):763-70.
1993: Oscar P Kuipers; M M Beerthuyzen; Roland Siezen; WILLEM DE VOS
Characterization of the nisin gene cluster nisABTCIPR of Lactococcus lactis. Requirement of expression of the nisA and nisI genes for development of immunity.
European journal of biochemistry / FEBS 1993;216(1):281-91.
1993: J R van der Meer; J Polman; M M Beerthuyzen; Roland Siezen; Oscar P Kuipers; WILLEM DE VOS
Characterization of the Lactococcus lactis nisin A operon genes nisP, encoding a subtilisin-like serine protease involved in precursor processing, and nisR, encoding a regulatory protein involved in nisin biosynthesis.
Journal of bacteriology 1993;175(9):2578-88.
1993: WILLEM DE VOS; J W Mulders; Roland Siezen; Jeroen Hugenholtz; Oscar P Kuipers
Properties of nisin Z and distribution of its gene, nisZ, in Lactococcus lactis.
Applied and environmental microbiology 1993;59(1):213-8.
1992: Oscar P Kuipers; H S Rollema; W M Yap; H J Boot; Roland Siezen; WILLEM DE VOS
Engineering dehydrated amino acid residues in the antimicrobial peptide nisin.
The Journal of biological chemistry 1992;267(34):24340-6.
1992: P S Tan; I J van Alen-Boerrigter; Bert Poolman; Roland Siezen; WILLEM DE VOS; Wil N Konings
Characterization of the Lactococcus lactis pepN gene encoding an aminopeptidase homologous to mammalian aminopeptidase N.
FEBS letters 1992;306(1):9-16.
1991: J W Mulders; I J Boerrigter; H S Rollema; Roland Siezen; WILLEM DE VOS
Identification and characterization of the lantibiotic nisin Z, a natural nisin variant.
European journal of biochemistry / FEBS 1991;201(3):581-4.
1991: Roland Siezen; WILLEM DE VOS; Jack Leunissen; B W Dijkstra
Homology modelling and protein engineering strategy of subtilases, the family of subtilisin-like serine proteinases.
Protein engineering 1991;4(7):719-37.
1991: P Vos; I J Boerrigter; Girbe Buist; A J Haandrikman; Monique Nijhuis; M B de Reuver; Roland Siezen; Gerard Venema; WILLEM DE VOS; Jan Kok
Engineering of the Lactococcus lactis serine proteinase by construction of hybrid enzymes.
Protein engineering 1991;4(4):479-84.
1990: Wim J M Van De Ven; Jan Voorberg; R Fontijn; H Pannekoek; Ans M W van den Ouweland; H L van Duijnhoven; Anton J M Roebroek; Roland Siezen
Furin is a subtilisin-like proprotein processing enzyme in higher eukaryotes.
Molecular biology reports 1990;14(4):265-75.
1990: Martien van Asseldonk; G Rutten; M Oteman; Roland Siezen; WILLEM DE VOS; Guus Simons
Cloning of usp45, a gene encoding a secreted protein from Lactococcus lactis subsp. lactis MG1363.
Gene 1990;95(1):155-60.
1990: P J van den Oetelaar; P F van Someren; J A Thomson; Roland Siezen; H J Hoenders
A dynamic quaternary structure of bovine alpha-crystallin as indicated from intermolecular exchange of subunits.
Biochemistry 1990;29(14):3488-93.
1989: P Vos; Guus Simons; Roland Siezen; WILLEM DE VOS
Primary structure and organization of the gene for a procaryotic, cell envelope-located serine proteinase.
The Journal of biological chemistry 1989;264(23):13579-85.
1989: Roland Siezen
Eye lens aging in the spiny dogfish (Squalus acanthias) I. Age determination from lens weight.
Current eye research 1989;8(7):707-12.
1989: P Vos; Martien van Asseldonk; F van Jeveren; Roland Siezen; Guus Simons; WILLEM DE VOS
A maturation protein is essential for production of active forms of Lactococcus lactis SK11 serine proteinase located in or secreted from the cell envelope.
Journal of bacteriology 1989;171(5):2795-802.
1989: Roland Siezen; C M Coppin; E D Kaplan; D Dwyer; J A Thomson
Oxidative modifications to crystallins induced in calf lenses in vitro by hydrogen peroxide.
Experimental eye research 1989;48(2):225-35.
1989: J A Thomson; Roland Siezen; E D Kaplan; M Messmer; B Chakrabarti
Comparative studies of beta s-crystallins from human, bovine, rat and rabbit lenses.
Current eye research 1989;8(2):139-49.
1988: Roland Siezen; E D Kaplan
Optimal resolution of eye lens gamma-crystallins by cation-exchange high-performance liquid chromatography on SynChropak CM300.
Journal of chromatography 1988;444():239-50.
1988: Roland Siezen
Reversible osmotic cataracts in spiny dogfish (Squalus acanthias) eye lens.
Experimental eye research 1988;46(6):987-90.
1988: Roland Siezen; E Wu; E D Kaplan; J A Thomson; George B. Benedek
Rat lens gamma-crystallins. Characterization of the six gene products and their spatial and temporal distribution resulting from differential synthesis.
Journal of molecular biology 1988;199(3):475-90.
1988: Roland Siezen; C Hom; E D Kaplan; J A Thomson; George B. Benedek
Heterogeneity of gamma-crystallins from spiny dogfish (Squalus acanthias) eye lens.
Experimental eye research 1988;46(1):81-93.
1987: Roland Siezen; J A Thomson; E D Kaplan; George B. Benedek
Human lens gamma-crystallins: isolation, identification, and characterization of the expressed gene products.
Proceedings of the National Academy of Sciences of the United States of America 1987;84(17):6088-92.
1987: K Mandal; B Chakrabarti; J Thomson; Roland Siezen
Structure and stability of gamma-crystallins. Denaturation and proteolysis behavior.
The Journal of biological chemistry 1987;262(17):8096-102.
1987: K Mandal; S K Bose; B Chakrabarti; Roland Siezen
Structure and stability of gamma-crystallins. II. Differences in microenvironments and spatial arrangements of cysteine residues.
Biochimica et biophysica acta 1987;911(3):277-84.
1986: Roland Siezen; R D Anello; J A Thomson
Interactions of lens proteins. Concentration dependence of beta-crystallin aggregation.
Experimental eye research 1986;43(3):293-303.
1985: K Mandal; S K Bose; B Chakrabarti; Roland Siezen
Structure and stability of gamma-crystallins. I. Spectroscopic evaluation of secondary and tertiary structure in solution.
Biochimica et biophysica acta 1985;832(2):156-64.
1985: Roland Siezen; C M Coppin; George B. Benedek
Permanent suppression of phase separation cataract in calf lens using amine modification agents.
Biochemical and biophysical research communications 1985;133(1):239-47.
1985: Roland Siezen; George B. Benedek
Controlled modulation of the phase separation and opacification temperature of purified bovine gamma IV-crystallin.
Current eye research 1985;4(10):1077-85.
1985: Roland Siezen; M R Fisch; C Slingsby; George B. Benedek
Opacification of gamma-crystallin solutions from calf lens in relation to cold cataract formation.
Proceedings of the National Academy of Sciences of the United States of America 1985;82(6):1701-5.
1985: Roland Siezen; E D Kaplan; R D Anello
Superior resolution of gamma-crystallins from microdissected eye lens by cation-exchange high-performance liquid chromatography.
Biochemical and biophysical research communications 1985;127(1):153-60.
1984: Roland Siezen; E F van Bruggen; M S Tai; M C Crossin; G Kegeles
Models for the analytical ultracentrifuge behavior of Helix pomatia alpha-hemocyanin. I. Experimental testing of previous models.
Biophysical chemistry 1984;19(2):99-112.
1984: Roland Siezen
Ultrafiltration studies of lens protein interactions. A re-evaluation.
Experimental eye research 1984;38(2):207-10.
1984: Roland Siezen
Interactions of lens proteins. Ultrafiltration is unsuitable to detect self- or mixed-association.
Biophysical chemistry 1984;19(1):49-55.
1983: Roland Siezen; E A Owen
Physicochemical characterization of high-molecular-weight alpha-crystallin subpopulations from the calf lens nucleus.
Biochimica et biophysica acta 1983;749(3):227-37.
1983: Roland Siezen; P Argos
Structural homology of lens crystallins. III. Secondary structure estimation from circular dichroism and prediction from amino acid sequences.
Biochimica et biophysica acta 1983;748(1):56-67.
1983: Roland Siezen; E A Owen; Y Kubota; T Ooi
Structural homology of lens crystallins. II. Homology expressed by correlation coefficients and hydropathy profiles.
Biochimica et biophysica acta 1983;748(1):48-55.
1983: Roland Siezen; E A Owen
Interactions of lens proteins. Self-association and mixed-association studies of bovine alpha-crystallin and gamma-crystallin.
Biophysical chemistry 1983;18(3):181-94.
1983: N Puri; R C Augusteyn; E A Owen; Roland Siezen
Immunochemical properties of vertebrate alpha-crystallins.
European journal of biochemistry / FEBS 1983;134(2):321-6.
1983: P Argos; Roland Siezen
Structural homology of lens crystallins. A method to detect protein structural homology from primary sequences.
European journal of biochemistry / FEBS 1983;131(1):143-8.
1982: Roland Siezen; D C Shaw
Physicochemical characterization of lens proteins of the squid Nototodarus gouldi and comparison with vertebrate crystallins.
Biochimica et biophysica acta 1982;704(2):304-20.
1982: Roland Siezen; J G Bindels
Stepwise dissociation/denaturation and reassociation/renaturation of bovine alpha-crystallin in urea and guanidine hydrochloride: sedimentation, fluorescence, near-ultraviolet and far-ultraviolet circular dichroism studies.
Experimental eye research 1982;34(6):969-83.
1982: Roland Siezen
Squid lens cytoskeleton and membrane proteins.
Current eye research 1982;2(9):579-89.
1981: Roland Siezen; L W Nichol; D J Winzor
Exclusion chromatography of concentrated hemoglobin solutions. Comparison of the self-association behavior of the oxy and deoxy forms of the alpha 2 beta 2 species.
Biophysical chemistry 1981;14(3):221-31.
1981: Roland Siezen
Reflections on the internal primary, secondary and tertiary structure homology of the eye lens proteins alpha-, beta- and gamma-crystallin.
FEBS letters 1981;133(1):1-8.
1980: Roland Siezen; J G Bindels; H J Hoenders
The quaternary structure of bovine alpha-crystallin. Effects of variation in alkaline pH, ionic strength, temperature and calcium ion concentration.
European journal of biochemistry / FEBS 1980;111(2):435-44.
1980: P R Wills; L W Nichol; Roland Siezen
The indefinite self-association of lysozyme: consideration of composition-dependent activity coefficients.
Biophysical chemistry 1980;11(1):71-82.
1980: Roland Siezen; J G Bindels; H J Hoenders
The quaternary structure of bovine alpha-crystallin. Chemical crosslinking with bifunctional imido esters.
European journal of biochemistry / FEBS 1980;107(1):243-9.
1979: L W Nichol; Roland Siezen; D J Winzor
Chromatographic evidence of the self-association of oxyhemoglobin in concentrated solutions: its biological implications.
Biophysical chemistry 1979;10(1):17-26.
1979: Roland Siezen; H J Hoenders
The quaternary structure of bovine alpha-crystallin. Surface probing by limited proteolysis in vitro.
European journal of biochemistry / FEBS 1979;96(3):431-40.
1979: Roland Siezen; J G Bindels; H J Hoenders
The interrelationship between monomeric, oligomeric and polymeric alpha-crystallin in the calf lens nucleus.
Experimental eye research 1979;28(5):551-67.
1979: Roland Siezen
Crosslinking of alpha-crystallin with bisimidoesters. Evidence for polyamidine formation at pH8 from an increase in positive charges on the polypeptide chains.
FEBS letters 1979;100(1):75-80.
1978: Roland Siezen; F G Coenders; H J Hoenders
Three classes of sulfhydryl group in bovine alpha-crystallin according to reactivity to various reagents.
Biochimica et biophysica acta 1978;537(2):456-65.
1978: Roland Siezen; J G Bindels; H J Hoenders
The quaternary structure of bovine alpha-crystallin. Size and charge microheterogeneity: more than 1000 different hybrids?
European journal of biochemistry / FEBS 1978;91(2):387-96.
1978: Roland Siezen; H Berger
The quaternary structure of bovine alpha-crystallin. Size and shape studies by sedimentation, small-angle X-ray scattering and quasi-elastic light scattering.
European journal of biochemistry / FEBS 1978;91(2):397-405.
1978: L W Nichol; Roland Siezen; D J Winzor
The study of multiple polymerization equilibria by glass bead exclusion chromatography with allowance for thermodynamic non-ideality effects.
Biophysical chemistry 1978;9(1):47-55.
1977: Roland Siezen; H J Hoenders
Limited tryptic digestion of alpha-crystallin from calf eye lens: Possible correlation between in vivo and in vitro degradation.
FEBS letters 1977;80(1):75-80.
1977: Roland Siezen; T H Mague
Gas-liquid chromatography of the N-heptafluorobutyryl isobutyl esters of fifty biologically interesting amino acids.
Journal of chromatography 1977;130():151-60.
1974: Roland Siezen; R van Driel
Structure and properties of hemocyanins. XIII. Dissociation of Helix pomatia alpha-hemocyanin at alkaline pH.
Journal of molecular biology 1974;90(1):91-102.
1974: Roland Siezen; E F van Bruggen
Structure and properties of hemocyanins. XII. Electron microscopy of dissociation products of Helix pomatia alpha-hemocyanin: quaternary structure.
Journal of molecular biology 1974;90(1):77-89.
1974: Roland Siezen
Structure and properties of hemocyanins. XIV. Reassociation of Helix pomatia alpha-hemocyanin.
Journal of molecular biology 1974;90(1):103-13.
1974: I Pilz; K Walder; Roland Siezen
Small-angle X-ray scattering studies of 1-10-size and 1-20-size molecules of Helix pomatia alpha-hemocyanin.
Zeitschrift für Naturforschung. Section C: Biosciences 1974;29(3):116-21.
1973: Roland Siezen; R van Driel
Structure and properties of hemocyanins. 8. Microheterogeneity of -hemocyanin of Helix pomatia.
Biochimica et biophysica acta 1973;295(1):131-9.
1969: W N Konings; Roland Siezen; M Gruber
Structure and properties of hemocyanins. VI. Association-dissociation behavior of Helix pomatia hemocyanin.
Biochimica et biophysica acta 1969;194(2):376-85.