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Gary J. Bassell

Research Profile

Chemicals & Drugs

Anatomy

Living Beings

Disorders

Fragile X Syndrome

Physiology

Protein Biosynthesis

Co-author Network

Publications

TOP 3
Sharon A Swanger; Yuncen A He; Joel D Richter; Gary J. Bassell
Dendritic GluN2A Synthesis Mediates Activity-Induced NMDA Receptor Insertion.
Lei Xing; Gary J. Bassell
mRNA localization: an orchestration of assembly, traffic and synthesis.
Vijayalaxmi Nalavadi; Philip Picard-Fraser; Andrew M Swanson; Toru Takumi; Laura E Griffin; Gary J. Bassell
Regulation of zipcode binding protein 1 transport dynamics in axons by myosin Va.

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All Publications

2013: Sharon A Swanger; Yuncen A He; Joel D Richter; Gary J. Bassell
Dendritic GluN2A Synthesis Mediates Activity-Induced NMDA Receptor Insertion.
The Journal of neuroscience : the official journal of the Society for Neuroscience 2013;33(20):8898-908.
2013: Lei Xing; Gary J. Bassell
mRNA localization: an orchestration of assembly, traffic and synthesis.
Traffic (Copenhagen, Denmark) 2013;14(1):2-14.
2012: Vijayalaxmi Nalavadi; Philip Picard-Fraser; Andrew M Swanson; Toru Takumi; Laura E Griffin; Gary J. Bassell
Regulation of zipcode binding protein 1 transport dynamics in axons by myosin Va.
The Journal of neuroscience : the official journal of the Society for Neuroscience 2012;32(43):15133-41.
2012: Claudia Fallini; Gary J. Bassell; wilfried rossoll
The ALS disease protein TDP-43 is actively transported in motor neuron axons and regulates axon outgrowth.
Human molecular genetics 2012;21(16):3703-18.
2012: Tsuyoshi Udagawa; R Suzanne Zukin; Jihae Shin; Sharon A Swanger; Koichi Takeuchi; Gary J. Bassell; Jong Heon Kim; Lori J Lorenz; Vijayalaxmi Nalavadi; Joel D Richter
Bidirectional control of mRNA translation and synaptic plasticity by the cytoplasmic polyadenylation complex.
Molecular cell 2012;47(2):253-66.
2012: Claudia Fallini; Gary J. Bassell; wilfried rossoll
Spinal muscular atrophy: the role of SMN in axonal mRNA regulation.
Brain research 2012;1462():81-92.
2012: Jennifer C Wilhelm; Mei Xu; Tiffany Holmes; Kelly Shukkwan Lau; Gary J. Bassell; Sarah Chmielewski; Delia Cucoranu; Arthur W English
Cooperative roles of BDNF expression in neurons and Schwann cells are modulated by exercise to facilitate nerve regeneration.
The Journal of neuroscience : the official journal of the Society for Neuroscience 2012;32(14):5002-9.
2012: Lei Xing; Xiaodi Yao; Kathryn R Williams; Gary J. Bassell
Negative regulation of RhoA translation and signaling by hnRNP-Q1 affects cellular morphogenesis.
Molecular biology of the cell 2012;23(8):1500-9.
2012: Vijayalaxmi Nalavadi; Christina Gross; Ravi S Muddashetty; Gary J. Bassell
Dephosphorylation-induced ubiquitination and degradation of FMRP in dendrites: a role in immediate early mGluR-stimulated translation.
The Journal of neuroscience : the official journal of the Society for Neuroscience 2012;32(8):2582-7.
2012: Eric B Dammer; Duc M Duong; Claudia Fallini; Yair M Gozal; James J Lah; Allan Levey; Junmin Peng; wilfried rossoll; Gary J. Bassell; Ping Xu; Nicholas T Seyfried
Coaggregation of RNA-binding proteins in a model of TDP-43 proteinopathy with selective RGG motif methylation and a role for RRM1 ubiquitination.
PloS one 2012;7(6):e38658.
2012: Marcello Ceci; Maria Teresa Ciotti; Francesca Paoletti; Chiara Parisi; Luana Pistillo; Kristy Welshhans; Gary J. Bassell; Rossella Brandi; Antonino Cattaneo
RACK1 is a ribosome scaffold protein for β-actin mRNA/ZBP1 complex.
PloS one 2012;7(4):e35034.
2012: Christina Gross; Gary J. Bassell
Excess protein synthesis in FXS patient lymphoblastoid cells can be rescued with a p110β-selective inhibitor.
Molecular medicine (Cambridge, Mass.) 2012;18():336-45.
2012: Christina Gross; Elizabeth M Berry-Kravis; Gary J. Bassell
Therapeutic strategies in fragile X syndrome: dysregulated mGluR signaling and beyond.
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology 2012;37(1):178-95.
2011: Gary J. Bassell
Fragile balance: RNA editing tunes the synapse.
Nature neuroscience 2011;14(12):1492-4.
2011: Christopher J Donnelly; Arthur W English; Sung Ok Yoon; Catherine B Kirn-Safran; Chunsu Jiang; Soonmoon Yoo; Mei Xu; Jan van Minnen; N Carolyn Schanen; Chhavy Tep; Dianna E Willis; Gary J. Bassell; Jeffery L Twiss
Limited availability of ZBP1 restricts axonal mRNA localization and nerve regeneration capacity.
The EMBO journal 2011;30(22):4665-77.
2011: Liqun Liu-Yesucevitz; Eric Klann; Stephen Warren; Anne C Hart; Gary J. Bassell; Aaron D Gitler; Joel D Richter; Benjamin Wolozin
Local RNA translation at the synapse and in disease.
The Journal of neuroscience : the official journal of the Society for Neuroscience 2011;31(45):16086-93.
2011: Dianna E Willis; Mei Xu; Sung Ok Yoon; Gary J. Bassell; Christopher J Donnelly; Arthur W English; Marina Erenstheyn; Marvin Kendall; Catherine B Kirn-Safran; N Carolyn Schanen; Chhavy Tep; Jeffery L Twiss
Axonal Localization of transgene mRNA in mature PNS and CNS neurons.
The Journal of neuroscience : the official journal of the Society for Neuroscience 2011;31(41):14481-7.
2011: Sharon A Swanger; Gary J. Bassell
Making and breaking synapses through local mRNA regulation.
Current opinion in genetics & development 2011;21(4):414-21.
2011: Changhui Pak; Hans-Hilger Ropers; Subhabrata Sanyal; Sara N Stahley; Andreas Tzschach; Kathryn R Williams; Sara W Leung; Guanglu Liu; Kenneth H Moberg; Marzieh Mohseni; Hossein Najmabadi; John J Noto; Seyedeh Sedigheh Abedini; Luciano H Apponi; Gary J. Bassell; Farkhondeh Behjati; Sharon Burdick; Anita H Corbett; Yue Feng; Masoud Garshasbi; Christina Gross; Hao Hu; Brenda Huang; Lars R Jensen; Kimia Kahrizi; Seth M Kelly; Andreas W Kuss
Mutation of the conserved polyadenosine RNA binding protein, ZC3H14/dNab2, impairs neural function in Drosophila and humans.
Proceedings of the National Academy of Sciences of the United States of America 2011;108(30):12390-5.
2011: Kristy Welshhans; Gary J. Bassell
Netrin-1-induced local β-actin synthesis and growth cone guidance requires zipcode binding protein 1.
The Journal of neuroscience : the official journal of the Society for Neuroscience 2011;31(27):9800-13.
2011: Ravi S Muddashetty; Vijayalaxmi C Nalavadi; Stephen Warren; Lei Xing; Xiaodi Yao; Christina Gross; Oskar Laur; Gary J. Bassell
Reversible inhibition of PSD-95 mRNA translation by miR-125a, FMRP phosphorylation, and mGluR signaling.
Molecular cell 2011;42(5):673-88.
2011: T N Ivanova; A Matthews; Christina Gross; R C Mappus; C Gollnick; A Swanson; Gary J. Bassell; Robert LIU
Arc/Arg3.1 mRNA expression reveals a subcellular trace of prior sound exposure in adult primary auditory cortex.
Neuroscience 2011;181():117-26.
2011: Cyril Jayakumar Peter; Matthew Evans; Venugopal Thayanithy; Naoko Taniguchi-Ishigaki; Ingolf Bach; Adrianne Kolpak; Gary J. Bassell; wilfried rossoll; Christian L Lorson; Zheng-Zheng Bao; Elliot J Androphy
The COPI vesicle complex binds and moves with survival motor neuron within axons.
Human molecular genetics 2011;20(9):1701-11.
2011: Christina Gross; Xiaodi Yao; Dan L Pong; Andreas Jeromin; Gary J. Bassell
Fragile X mental retardation protein regulates protein expression and mRNA translation of the potassium channel Kv4.2.
The Journal of neuroscience : the official journal of the Society for Neuroscience 2011;31(15):5693-8.
2011: Claudia Fallini; Honglai Zhang; Yuehang Su; Vincenzo Silani; Robert H Singer; wilfried rossoll; Gary J. Bassell
The survival of motor neuron (SMN) protein interacts with the mRNA-binding protein HuD and regulates localization of poly(A) mRNA in primary motor neuron axons.
The Journal of neuroscience : the official journal of the Society for Neuroscience 2011;31(10):3914-25.
2011: Liang Han; Christine E Holt; Rachel C Lynn; Yukio Sasaki; Zhexing Wen; Gary J. Bassell; Marie-Laure Baudet; James Q ZHENG
Regulation of chemotropic guidance of nerve growth cones by microRNA.
Molecular brain 2011;4():40.
2011: Sharon A Swanger; Xiaodi Yao; Christina Gross; Gary J. Bassell
Automated 4D analysis of dendritic spine morphology: applications to stimulus-induced spine remodeling and pharmacological rescue in a disease model.
Molecular brain 2011;4():38.
2011: Sharon A Swanger; Gary J. Bassell; Christina Gross
High-resolution fluorescence in situ hybridization to detect mRNAs in neuronal compartments in vitro and in vivo.
Methods in molecular biology (Clifton, N.J.) 2011;714():103-23.
2010: Ming-Chung Kan; Aparna Oruganty-Das; Amalene Cooper-Morgan; Guang Jin; Sharon A Swanger; Gary J. Bassell; Harvey Florman; Klaus Van Leyen; Joel D Richter
CPEB4 is a cell survival protein retained in the nucleus upon ischemia or endoplasmic reticulum calcium depletion.
Molecular and cellular biology 2010;30(24):5658-71.
2010: Christina Gross; Mika Nakamoto; Xiaodi Yao; Chi-Bun Chan; So Y Yim; Keqiang Ye; Stephen Warren; Gary J. Bassell
Excess phosphoinositide 3-kinase subunit synthesis and activity as a novel therapeutic target in fragile X syndrome.
The Journal of neuroscience : the official journal of the Society for Neuroscience 2010;30(32):10624-38.
2010: Yukio Sasaki; Kristy Welshhans; Zhexing Wen; Jiaqi Yao; Mei Xu; Yoshio Goshima; James Q ZHENG; Gary J. Bassell
Phosphorylation of zipcode binding protein 1 is required for brain-derived neurotrophic factor signaling of local beta-actin synthesis and growth cone turning.
The Journal of neuroscience : the official journal of the Society for Neuroscience 2010;30(28):9349-58.
2010: Claudia Fallini; Gary J. Bassell; Wilfried Rossoll
High-efficiency transfection of cultured primary motor neurons to study protein localization, trafficking, and function.
Molecular neurodegeneration 2010;5():17.
2009: Philip J Santangelo; Aaron W Lifland; Paul Curt; Yukio Sasaki; Gary J. Bassell; Michael E Lindquist; James Crowe
Single molecule-sensitive probes for imaging RNA in live cells.
Nature methods 2009;6(5):347-9.
2009: Ravi Muddashetty; Gary J. Bassell
A boost in microRNAs shapes up the neuron.
The EMBO journal 2009;28(6):617-8.
2009: Chanxia Li; Gary J. Bassell; Yukio Sasaki
Fragile X Mental Retardation Protein is Involved in Protein Synthesis-Dependent Collapse of Growth Cones Induced by Semaphorin-3A.
Frontiers in neural circuits 2009;3():11.
2009: Wilfried Rossoll; Gary J. Bassell
Spinal muscular atrophy and a model for survival of motor neuron protein function in axonal ribonucleoprotein complexes.
Results and problems in cell differentiation 2009;48():289-326.
2008: Gary J. Bassell; Stephen Warren
Fragile X syndrome: loss of local mRNA regulation alters synaptic development and function.
Neuron 2008;60(2):201-14.
2008: Usha Narayanan; Vijayalaxmi Nalavadi; Mika Nakamoto; George Thomas; Stephanie Ceman; Gary J. Bassell; Stephen Warren
S6K1 phosphorylates and regulates fragile X mental retardation protein (FMRP) with the neuronal protein synthesis-dependent mammalian target of rapamycin (mTOR) signaling cascade.
The Journal of biological chemistry 2008;283(27):18478-82.
2008: Jason B Dictenberg; Sharon A Swanger; Laura N Antar; Robert H Singer; Gary J. Bassell
A direct role for FMRP in activity-dependent dendritic mRNA transport links filopodial-spine morphogenesis to fragile X syndrome.
Developmental cell 2008;14(6):926-39.
2008: Gabriela E Oprea; Sandra Kröber; Michelle L McWhorter; wilfried rossoll; Stefan Müller; Michael Krawczak; Gary J. Bassell; Christine E Beattie; Brunhilde Wirth
Plastin 3 is a protective modifier of autosomal recessive spinal muscular atrophy.
Science (New York, N.Y.) 2008;320(5875):524-7.
2008: Gary J. Bassell; Christina Gross
Reducing glutamate signaling pays off in fragile X.
Nature medicine 2008;14(3):249-50.
2008: Houping Wang; Jason B Dictenberg; Li Ku; Wen Li; Gary J. Bassell; Yue Feng
Dynamic association of the fragile X mental retardation protein as a messenger ribonucleoprotein between microtubules and polyribosomes.
Molecular biology of the cell 2008;19(1):105-14.
2007: Usha Narayanan; Vijayalaxmi Nalavadi; Mika Nakamoto; David C Pallas; Stephanie Ceman; Gary J. Bassell; Stephen Warren
FMRP phosphorylation reveals an immediate-early signaling pathway triggered by group I mGluR and mediated by PP2A.
The Journal of neuroscience : the official journal of the Society for Neuroscience 2007;27(52):14349-57.
2007: Mika Nakamoto; Vijayalaxmi Nalavadi; Michael P Epstein; Usha Narayanan; Gary J. Bassell; Stephen Warren
Fragile X mental retardation protein deficiency leads to excessive mGluR5-dependent internalization of AMPA receptors.
Proceedings of the National Academy of Sciences of the United States of America 2007;104(39):15537-42.
2007: Dianna E Willis; Erna A van Niekerk; Yukio Sasaki; Mariano Mesngon; Tanuja T Merianda; Gervan Williams; Marvin Kendall; Deanna S Smith; Gary J. Bassell; Jeffery L Twiss
Extracellular stimuli specifically regulate localized levels of individual neuronal mRNAs.
The Journal of cell biology 2007;178(6):965-80.
2007: Honglai Zhang; Lei Xing; Robert H Singer; Gary J. Bassell
QNQKE targeting motif for the SMN-Gemin multiprotein complexin neurons.
Journal of neuroscience research 2007;85(12):2657-67.
2007: Ravi S Muddashetty; Sofija Kelić; Christina Gross; Mei Xu; Gary J. Bassell
Dysregulated metabotropic glutamate receptor-dependent translation of AMPA receptor and postsynaptic density-95 mRNAs at synapses in a mouse model of fragile X syndrome.
The Journal of neuroscience : the official journal of the Society for Neuroscience 2007;27(20):5338-48.
2006: Tessa L Carrel; Michelle L McWhorter; Eileen Workman; Honglai Zhang; Elizabeth C Wolstencroft; Christian L Lorson; Gary J. Bassell; Arthur H M Burghes; Christine E Beattie
Survival motor neuron function in motor axons is independent of functions required for small nuclear ribonucleoprotein biogenesis.
The Journal of neuroscience : the official journal of the Society for Neuroscience 2006;26(43):11014-22.
2006: Jiaqi Yao; Yukio Sasaki; Zhexing Wen; Gary J. Bassell; James Q ZHENG
An essential role for beta-actin mRNA localization and translation in Ca2+-dependent growth cone guidance.
Nature neuroscience 2006;9(10):1265-73.
2006: Michael A Kiebler; Gary J. Bassell
Neuronal RNA granules: movers and makers.
Neuron 2006;51(6):685-90.
2006: Honglai Zhang; Lei Xing; wilfried rossoll; Hynek Wichterle; Robert H Singer; Gary J. Bassell
Multiprotein complexes of the survival of motor neuron protein SMN with Gemins traffic to neuronal processes and growth cones of motor neurons.
The Journal of neuroscience : the official journal of the Society for Neuroscience 2006;26(33):8622-32.
2006: Sonja Y Grooms; Kyung-Min Noh; Roodland Regis; Gary J. Bassell; Monique K Bryan; Carroll Reed; R Suzanne Zukin
Activity bidirectionally regulates AMPA receptor mRNA abundance in dendrites of hippocampal neurons.
The Journal of neuroscience : the official journal of the Society for Neuroscience 2006;26(32):8339-51.
2006: Laura N Antar; Chanxia Li; Honglai Zhang; Carroll Reed; Gary J. Bassell
Local functions for FMRP in axon growth cone motility and activity-dependent regulation of filopodia and spine synapses.
Molecular and cellular neurosciences 2006;32(1-2):37-48.
2006: Gary J. Bassell; Jeffery L Twiss
RNA exodus to Israel: RNA controlling function in the far reaches of the neuron. Workshop on RNA control on neuronal function.
EMBO reports 2006;7(1):31-5.
2005: Stefan Hüttelmaier; Daniel Zenklusen; Marcell Lederer; Jason B Dictenberg; Mike Lorenz; Xiuhua Meng; Gary J. Bassell; John Condeelis; Robert H Singer
Spatial regulation of beta-actin translation by Src-dependent phosphorylation of ZBP1.
Nature 2005;438(7067):512-5.
2005: Laura N Antar; Jason B Dictenberg; M Plociniak; Rownak Afroz; Gary J. Bassell
Localization of FMRP-associated mRNA granules and requirement of microtubules for activity-dependent trafficking in hippocampal neurons.
Genes, brain, and behavior 2005;4(6):350-9.
2005: Thanh T Le; Lan T Pham; Matthew E R Butchbach; Honglai L Zhang; Umrao R Monani; Daniel D Coovert; Tatiana O Gavrilina; Lei Xing; Gary J. Bassell; Arthur H M Burghes
SMNDelta7, the major product of the centromeric survival motor neuron (SMN2) gene, extends survival in mice with spinal muscular atrophy and associates with full-length SMN.
Human molecular genetics 2005;14(6):845-57.
2004: Catherine L Smith; Rownak Afroz; Gary J. Bassell; Henry Furneaux; Nora I Perrone-Bizzozero; Richard W Burry
GAP-43 mRNA in growth cones is associated with HuD and ribosomes.
Journal of neurobiology 2004;61(2):222-35.
2004: Gary J. Bassell; Sofija Kelic
Binding proteins for mRNA localization and local translation, and their dysfunction in genetic neurological disease.
Current opinion in neurobiology 2004;14(5):574-81.
2004: Laura N Antar; Rownak Afroz; Jason B Dictenberg; Carroll Reed; Gary J. Bassell
Metabotropic glutamate receptor activation regulates fragile x mental retardation protein and FMR1 mRNA localization differentially in dendrites and at synapses.
The Journal of neuroscience : the official journal of the Society for Neuroscience 2004;24(11):2648-55.
2004: Rob Willemsen; Ben A Oostra; Gary J. Bassell; Jason B Dictenberg
The fragile X syndrome: from molecular genetics to neurobiology.
Mental retardation and developmental disabilities research reviews 2004;10(1):60-7.
2003: Taesun Eom; Laura N Antar; Robert H Singer; Gary J. Bassell
Localization of a beta-actin messenger ribonucleoprotein complex with zipcode-binding protein modulates the density of dendritic filopodia and filopodial synapses.
The Journal of neuroscience : the official journal of the Society for Neuroscience 2003;23(32):10433-44.
2003: Honglai L Zhang; Feng Pan; Daewha Hong; Shailesh M Shenoy; Robert H Singer; Gary J. Bassell
Active transport of the survival motor neuron protein and the role of exon-7 in cytoplasmic localization.
The Journal of neuroscience : the official journal of the Society for Neuroscience 2003;23(16):6627-37.
2003: Dhanrajan M Tiruchinapalli; Yuri Oleynikov; Sofija Kelic; Shailesh M Shenoy; Adam Hartley; Patric Stanton; Robert H Singer; Gary J. Bassell
Activity-dependent trafficking and dynamic localization of zipcode binding protein 1 and beta-actin mRNA in dendrites and spines of hippocampal neurons.
The Journal of neuroscience : the official journal of the Society for Neuroscience 2003;23(8):3251-61.
2003: Laura N Antar; Gary J. Bassell
Sunrise at the synapse: the FMRP mRNP shaping the synaptic interface.
Neuron 2003;37(4):555-8.
2002: Wei Gu; Feng Pan; Honglai Zhang; Gary J. Bassell; Robert H Singer
A predominantly nuclear protein affecting cytoplasmic localization of beta-actin mRNA in fibroblasts and neurons.
The Journal of cell biology 2002;156(1):41-51.
2001: Honglai L Zhang; T Eom; Yuri Oleynikov; Shailesh M Shenoy; D A Liebelt; Jason B Dictenberg; Robert H Singer; Gary J. Bassell
Neurotrophin-induced transport of a beta-actin mRNP complex increases beta-actin levels and stimulates growth cone motility.
Neuron 2001;31(2):261-75.
2001: Gary J. Bassell; Robert H Singer
Neuronal RNA localization and the cytoskeleton.
Results and problems in cell differentiation 2001;34():41-56.
1999: H L Zhang; Robert H Singer; Gary J. Bassell
Neurotrophin regulation of beta-actin mRNA and protein localization within growth cones.
The Journal of cell biology 1999;147(1):59-70.
1999: Gary J. Bassell; Yuri Oleynikov; Robert H Singer
The travels of mRNAs through all cells large and small.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 1999;13(3):447-54.
1998: T Ikezu; H Ueda; Bruce D Trapp; Kazutoshi Nishiyama; J F Sha; Daniela Volonte; Ferruccio Galbiati; A L Byrd; Gary J. Bassell; H Serizawa; William Stratton Lane; Michael P Lisanti; T Okamoto
Affinity-purification and characterization of caveolins from the brain: differential expression of caveolin-1, -2, and -3 in brain endothelial and astroglial cell types.
Brain research 1998;804(2):177-92.
1998: Gary J. Bassell; Honglai Zhang; A L Byrd; Andrea M Femino; Robert H Singer; K L Taneja; Lawrence Lifshitz; I M Herman; Kenneth S Kosik
Sorting of beta-actin mRNA and protein to neurites and growth cones in culture.
The Journal of neuroscience : the official journal of the Society for Neuroscience 1998;18(1):251-65.
1997: Gary J. Bassell; Robert H Singer
mRNA and cytoskeletal filaments.
Current opinion in cell biology 1997;9(1):109-15.
1996: R B Knowles; J H Sabry; Maryann E Martone; Thomas J Deerinck; M H Ellisman; Gary J. Bassell; Kenneth S Kosik
Translocation of RNA granules in living neurons.
The Journal of neuroscience : the official journal of the Society for Neuroscience 1996;16(24):7812-20.
1994: Mohan Somasundaran; Maria L Zapp; L K Beattie; L Pang; K S Byron; Gary J. Bassell; John L Sullivan; Robert H Singer
Localization of HIV RNA in mitochondria of infected cells: potential role in cytopathogenicity.
The Journal of cell biology 1994;126(6):1353-60.
1994: Gary J. Bassell; C M Powers; K L Taneja; Robert H Singer
Single mRNAs visualized by ultrastructural in situ hybridization are principally localized at actin filament intersections in fibroblasts.
The Journal of cell biology 1994;126(4):863-76.
1994: Gary J. Bassell; Robert H Singer; Kenneth S Kosik
Association of poly(A) mRNA with microtubules in cultured neurons.
Neuron 1994;12(3):571-82.
1994: Gary J. Bassell; K L Taneja; Edward Kislauskis; C L Sundell; C M Powers; A Ross; Robert H Singer
Actin filaments and the spatial positioning of mRNAS.
Advances in experimental medicine and biology 1994;358():183-9.
1993: Gary J. Bassell
High resolution distribution of mRNA within the cytoskeleton.
Journal of cellular biochemistry 1993;52(2):127-33.