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James Cregg

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Volodymyr Y Nazarko; Andriy A Sibirny; Oleh V Stasyk; James M Cregg; Jean-Claude Farré; Taras Y Nazarko; Stanislaw Ulaszewski; Dirk Warnecke; Suresh Subramani
Atg35, a micropexophagy-specific protein that regulates micropexophagic apparatus formation in Pichia pastoris.
Tamara Wriessnegger; Anthony Jay Sunga; James M Cregg; Günther Daum
Identification of phosphatidylserine decarboxylases 1 and 2 from Pichia pastoris.
James M Cregg; Ilya Tolstorukov; Anasua Kusari; Jay Sunga; Knut Madden; Thomas Chappell
Expression in the yeast Pichia pastoris.

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All Publications

2011: Volodymyr Y Nazarko; Andriy A Sibirny; Oleh V Stasyk; James M Cregg; Jean-Claude Farré; Taras Y Nazarko; Stanislaw Ulaszewski; Dirk Warnecke; Suresh Subramani
Atg35, a micropexophagy-specific protein that regulates micropexophagic apparatus formation in Pichia pastoris.
Autophagy 2011;7(4):375-85.
2009: Tamara Wriessnegger; Anthony Jay Sunga; James M Cregg; Günther Daum
Identification of phosphatidylserine decarboxylases 1 and 2 from Pichia pastoris.
FEMS yeast research 2009;9(6):911-22.
2009: James M Cregg; Ilya Tolstorukov; Anasua Kusari; Jay Sunga; Knut Madden; Thomas Chappell
Expression in the yeast Pichia pastoris.
Methods in enzymology 2009;463():169-89.
2008: Anthony Jay Sunga; Ilya Tolstorukov; James M Cregg
Posttransformational vector amplification in the yeast Pichia pastoris.
FEMS yeast research 2008;8(6):870-6.
2008: Franz S Hartner; Claudia Ruth; David Langenegger; Sabrina N Johnson; Petr Hyka; Geoffrey P Lin-Cereghino; Joan Lin-Cereghino; Karin Kovar; James M Cregg; Anton Glieder
Promoter library designed for fine-tuned gene expression in Pichia pastoris.
Nucleic acids research 2008;36(12):e76.
2007: Tamara Wriessnegger; Georg M Gübitz; Erich Leitner; Elisabeth Ingolic; James M Cregg; Bernard J de la Cruz; Günther Daum
Lipid composition of peroxisomes from the yeast Pichia pastoris grown on different carbon sources.
Biochimica et biophysica acta 2007;1771(4):455-61.
2007: Leonardo M Damasceno; Kyle A Anderson; Gerd Ritter; James M Cregg; Lloyd J Old; Carl A Batt
Cooverexpression of chaperones for enhanced secretion of a single-chain antibody fragment in Pichia pastoris.
Applied microbiology and biotechnology 2007;74(2):381-9.
2007: Ilya Tolstorukov; James M Cregg
Classical genetics.
Methods in molecular biology (Clifton, N.J.) 2007;389():189-202.
2007: James M Cregg
DNA-mediated transformation.
Methods in molecular biology (Clifton, N.J.) 2007;389():27-42.
2007: James M Cregg
Introduction: distinctions between Pichia pastoris and other expression systems.
Methods in molecular biology (Clifton, N.J.) 2007;389():1-10.
2006: Philipp Ternes; Petra Sperling; Sandra Albrecht; Stephan Franke; James M Cregg; Dirk Warnecke; Ernst Heinz
Identification of fungal sphingolipid C9-methyltransferases by phylogenetic profiling.
The Journal of biological chemistry 2006;281(9):5582-92.
2006: Geoff P Lin-Cereghino; Laurie Godfrey; Bernard J de la Cruz; Sabrina Johnson; Samone Khuongsathiene; Ilya Tolstorukov; Mingda Yan; Joan Lin-Cereghino; Marten Veenhuis; Suresh Subramani; James M Cregg
Mxr1p, a key regulator of the methanol utilization pathway and peroxisomal genes in Pichia pastoris.
Molecular and cellular biology 2006;26(3):883-97.
2006: Sébastien Léon; Lan Zhang; W Hayes McDonald; John Yates; James M Cregg; Suresh Subramani
Dynamics of the peroxisomal import cycle of PpPex20p: ubiquitin-dependent localization and regulation.
The Journal of cell biology 2006;172(1):67-78.
2006: Oleh V Stasyk; Olena G Stasyk; Richard D Mathewson; Jean-Claude Farré; Volodymyr Y Nazarko; Olena S Krasovska; Suresh Subramani; James M Cregg; Andriy A Sibirny
Atg28, a novel coiled-coil protein involved in autophagic degradation of peroxisomes in the methylotrophic yeast Pichia pastoris.
Autophagy 2006;2(1):30-8.
2005: Gerd Gellissen; Gotthard Kunze; Claude Gaillardin; James M Cregg; Enrico Berardi; Marten Veenhuis; Ida Van der klei
New yeast expression platforms based on methylotrophic Hansenula polymorpha and Pichia pastoris and on dimorphic Arxula adeninivorans and Yarrowia lipolytica - a comparison.
FEMS yeast research 2005;5(11):1079-96.
2005: William A Dunn; James M Cregg; Jan A K W Kiel; Ida Van der klei; Masahide Oku; Yasuyoshi Sakai; Andriy A Sibirny; Oleh V Stasyk; Marten Veenhuis
Pexophagy: the selective autophagy of peroxisomes.
Autophagy 2005;1(2):75-83.
2005: Der Thor; See Xiong; Claire C Orazem; An-Chun Kwan; James M Cregg; Joan Lin-Cereghino; Geoff P Lin-Cereghino
Cloning and characterization of the Pichia pastoris MET2 gene as a selectable marker.
FEMS yeast research 2005;5(10):935-42.
2005: Oriol Cos; Alícia Serrano; José Luis Montesinos; Pau Ferrer; James M Cregg; Francisco Valero
Combined effect of the methanol utilization (Mut) phenotype and gene dosage on recombinant protein production in Pichia pastoris fed-batch cultures.
Journal of biotechnology 2005;116(4):321-35.
2005: Dominic Esposito; William K Gillette; David A Miller; Troy E Taylor; Peter H Frank; Renqui Hu; Joseph Bekisz; Jessica Hernandez; James M Cregg; Kathy Zoon; James L Hartley
Gateway cloning is compatible with protein secretion from Pichia pastoris.
Protein expression and purification 2005;40(2):424-8.
2004: Anthony Jay Sunga; James M Cregg
The Pichia pastoris formaldehyde dehydrogenase gene (FLD1) as a marker for selection of multicopy expression strains of P. pastoris.
Gene 2004;330():39-47.
2004: Oleh V Stasyk; Olena G Stasyk; Janet Komduur; Marten Veenhuis; James M Cregg; Andriy A Sibirny
A hexose transporter homologue controls glucose repression in the methylotrophic yeast Hansenula polymorpha.
The Journal of biological chemistry 2004;279(9):8116-25.
2003: Daniel J Klionsky; James M Cregg; William A Dunn; Scott D Emr; Yasuyoshi Sakai; Ignacio V Sandoval; Andriy A Sibirny; Suresh Subramani; Michael Thumm; Marten Veenhuis; Yoshinori Ohsumi
A unified nomenclature for yeast autophagy-related genes.
Developmental cell 2003;5(4):539-45.
2003: Oleh V Stasyk; Taras Y Nazarko; Olena G Stasyk; Olena S Krasovska; Dirk Warnecke; Jean-Marc Nicaud; James M Cregg; Andriy A Sibirny
Sterol glucosyltransferases have different functional roles in Pichia pastoris and Yarrowia lipolytica.
Cell biology international 2003;27(11):947-52.
2002: Geoff P Lin Cereghino; Joan Lin Cereghino; Christine Ilgen; James M Cregg
Production of recombinant proteins in fermenter cultures of the yeast Pichia pastoris.
Current opinion in biotechnology 2002;13(4):329-32.
2002: Richard J S Baerends; Grietje J Sulter; Thomas W Jeffries; James M Cregg; Marten Veenhuis
Molecular characterization of the Hansenula polymorpha FLD1 gene encoding formaldehyde dehydrogenase.
Yeast (Chichester, England) 2002;19(1):37-42.
2001: MA Johnson; William B Snyder; Joan Lin Cereghino; Marten Veenhuis; Suresh Subramani; James M Cregg
Pichia pastoris Pex14p, a phosphorylated peroxisomal membrane protein, is part of a PTS-receptor docking complex and interacts with many peroxins.
Yeast (Chichester, England) 2001;18(7):621-41.
2001: Geoff P Lin-Cereghino; Joan Lin-Cereghino; Anthony Jay Sunga; MA Johnson; M Lim; M A Gleeson; James M Cregg
New selectable marker/auxotrophic host strain combinations for molecular genetic manipulation of Pichia pastoris.
Gene 2001;263(1-2):159-69.
2001: Geoff P Lin-Cereghino; Anthony Jay Sunga; Joan Lin-Cereghino; James M Cregg
Expression of foreign genes in the yeast Pichia pastoris.
Genetic engineering 2001;23():157-69.
2000: James M Cregg; Joan Lin Cereghino; J Shi; DR Higgins
Recombinant protein expression in Pichia pastoris.
Molecular biotechnology 2000;16(1):23-52.
2000: Joan Lin Cereghino; James M Cregg
Heterologous protein expression in the methylotrophic yeast Pichia pastoris.
FEMS microbiology reviews 2000;24(1):45-66.
1999: William B Snyder; Antonius Koller; A J Choy; MA Johnson; James M Cregg; Linda Rangell; Gilbert A Keller; Suresh Subramani
Pex17p is required for import of both peroxisome membrane and lumenal proteins and interacts with Pex19p and the peroxisome targeting signal-receptor docking complex in Pichia pastoris.
Molecular biology of the cell 1999;10(12):4005-19.
1999: Oleh V Stasyk; Ida Van der klei; Anna Rita Bellu; S Shen; Jan A K W Kiel; James M Cregg; Marten Veenhuis
A Pichia pastoris VPS15 homologue is required in selective peroxisome autophagy.
Current genetics 1999;36(5):262-9.
1999: G P Cereghino; James M Cregg
Applications of yeast in biotechnology: protein production and genetic analysis.
Current opinion in biotechnology 1999;10(5):422-7.
1999: Jan A K W Kiel; RE Hilbrands; Ida Van der klei; SW Rasmussen; Florian A Salomons; Meis van der Heide; Klaas Nico Faber; James M Cregg; Marten Veenhuis
Hansenula polymorpha Pex1p and Pex6p are peroxisome-associated AAA proteins that functionally and physically interact.
Yeast (Chichester, England) 1999;15(11):1059-78.
1999: MA Johnson; HR Waterham; G P Ksheminska; Lyubov R Fayura; Joan Lin Cereghino; Oleh V Stasyk; Marten Veenhuis; A R Kulachkovsky; Andriy A Sibirny; James M Cregg
Positive selection of novel peroxisome biogenesis-defective mutants of the yeast Pichia pastoris.
Genetics 1999;151(4):1379-91.
1998: S Shen; G Sulter; T W Jeffries; James M Cregg
A strong nitrogen source-regulated promoter for controlled expression of foreign genes in the yeast Pichia pastoris.
Gene 1998;216(1):93-102.
1998: Jianying Shi; Hongfa Zhu; Dennis N Arvidson; James M Cregg; Gebre Woldegiorgis
Deletion of the conserved first 18 N-terminal amino acid residues in rat liver carnitine palmitoyltransferase I abolishes malonyl-CoA sensitivity and binding.
Biochemistry 1998;37(31):11033-8.
1998: Ida Van der klei; RE Hilbrands; Jan A K W Kiel; SW Rasmussen; James M Cregg; Marten Veenhuis
The ubiquitin-conjugating enzyme Pex4p of Hansenula polymorpha is required for efficient functioning of the PTS1 import machinery.
The EMBO journal 1998;17(13):3608-18.
1998: James M Cregg; K A Russell
Transformation.
Methods in molecular biology (Clifton, N.J.) 1998;103():27-39.
1998: James M Cregg; S Shen; M Johnson; HR Waterham
Classical genetic manipulation.
Methods in molecular biology (Clifton, N.J.) 1998;103():17-26.
1998: DR Higgins; James M Cregg
Introduction to Pichia pastoris.
Methods in molecular biology (Clifton, N.J.) 1998;103():1-15.
1997: HR Waterham; K A Russell; Y Vries; James M Cregg
Peroxisomal targeting, import, and assembly of alcohol oxidase in Pichia pastoris.
The Journal of cell biology 1997;139(6):1419-31.
1997: Hongfa Zhu; Jianying Shi; Y de Vries; Dennis N Arvidson; James M Cregg; Gebre Woldegiorgis
Functional studies of yeast-expressed human heart muscle carnitine palmitoyltransferase I.
Archives of biochemistry and biophysics 1997;347(1):53-61.
1997: Hongfa Zhu; Jianying Shi; James M Cregg; Gebre Woldegiorgis
Reconstitution of highly expressed human heart muscle carnitine palmitoyltransferase I.
Biochemical and biophysical research communications 1997;239(2):498-502.
1997: Y de Vries; Dennis N Arvidson; H R Waterham; James M Cregg; Gebre Woldegiorgis
Functional characterization of mitochondrial carnitine palmitoyltransferases I and II expressed in the yeast Pichia pastoris.
Biochemistry 1997;36(17):5285-92.
1997: H R Waterham; M E Digan; P J Koutz; S V Lair; James M Cregg
Isolation of the Pichia pastoris glyceraldehyde-3-phosphate dehydrogenase gene and regulation and use of its promoter.
Gene 1997;186(1):37-44.
1997: M Komori; SW Rasmussen; Jan A K W Kiel; Richard J S Baerends; James M Cregg; Ida Van der klei; Marten Veenhuis
The Hansenula polymorpha PEX14 gene encodes a novel peroxisomal membrane protein essential for peroxisome biogenesis.
The EMBO journal 1997;16(1):44-53.
1997: H R Waterham; James M Cregg
Peroxisome biogenesis.
BioEssays : news and reviews in molecular, cellular and developmental biology 1997;19(1):57-66.
1996: H R Waterham; Y De Vries; K A Russell; W Xie; Marten Veenhuis; James M Cregg
The Pichia pastoris PER6 gene encodes the yeast homologue of human PAF-1.
Annals of the New York Academy of Sciences 1996;804():773-4.
1996: Ben Distel; Ralf Erdmann; Stephen J Gould; Günter Blobel; D I Crane; James M Cregg; Gabriele Dodt; Y Fujiki; J M Goodman; W W Just; Jan A K W Kiel; Wolf-H Kunau; Paul B LAZAROW; Guy P Mannaerts; Hugo W Moser; T Osumi; R A Rachubinski; A Roscher; Suresh Subramani; Henk F Tabak; T Tsukamoto; David Valle; Ida Van der klei; P P van Veldhoven; M Veenhuis
A unified nomenclature for peroxisome biogenesis factors.
The Journal of cell biology 1996;135(1):1-3.
1996: H R Waterham; Y de Vries; K A Russel; W Xie; Marten Veenhuis; James M Cregg
The Pichia pastoris PER6 gene product is a peroxisomal integral membrane protein essential for peroxisome biogenesis and has sequence similarity to the Zellweger syndrome protein PAF-1.
Molecular and cellular biology 1996;16(5):2527-36.
1996: Richard J S Baerends; S W Rasmussen; R E Hilbrands; Meis van der Heide; Klaas Nico Faber; P T Reuvekamp; Jan A K W Kiel; James M Cregg; Ida Van der klei; Marten Veenhuis
The Hansenula polymorpha PER9 gene encodes a peroxisomal membrane protein essential for peroxisome assembly and integrity.
The Journal of biological chemistry 1996;271(15):8887-94.
1995: X Tan; V I Titorenko; Ida Van der klei; G J Sulter; Peter Haima; H R Waterham; M Eyers; W Harder; Marten Veenhuis; James M Cregg
Characterization of peroxisome-deficient mutants of Hansenula polymorpha.
Current genetics 1995;28(3):248-57.
1995: Ida Van der klei; R E Hilbrands; G J Swaving; H R Waterham; E G Vrieling; V I Titorenko; James M Cregg; W Harder; Marten Veenhuis
The Hansenula polymorpha PER3 gene is essential for the import of PTS1 proteins into the peroxisomal matrix.
The Journal of biological chemistry 1995;270(29):17229-36.
1995: H Liu; X Tan; K A Russell; Marten Veenhuis; James M Cregg
PER3, a gene required for peroxisome biogenesis in Pichia pastoris, encodes a peroxisomal membrane protein involved in protein import.
The Journal of biological chemistry 1995;270(18):10940-51.
1995: X Tan; H R Waterham; Marten Veenhuis; James M Cregg
The Hansenula polymorpha PER8 gene encodes a novel peroxisomal integral membrane protein involved in proliferation.
The Journal of cell biology 1995;128(3):307-19.
1994: H R Waterham; V I Titorenko; Peter Haima; James M Cregg; W Harder; Marten Veenhuis
The Hansenula polymorpha PER1 gene is essential for peroxisome biogenesis and encodes a peroxisomal matrix protein with both carboxy- and amino-terminal targeting signals.
The Journal of cell biology 1994;127(3):737-49.
1993: V I Titorenko; H R Waterham; James M Cregg; W Harder; Marten Veenhuis
Peroxisome biogenesis in the yeast Hansenula polymorpha is controlled by a complex set of interacting gene products.
Proceedings of the National Academy of Sciences of the United States of America 1993;90(16):7470-4.
1991: M J de Hoop; James M Cregg; I Keizer-Gunnink; K Sjollema; Marten Veenhuis; G Ab
Overexpression of alcohol oxidase in Pichia pastoris.
FEBS letters 1991;291(2):299-302.
1991: M de Hoop; S Asgeirsdottir; Mieke Blaauw; Marten Veenhuis; James M Cregg; M Gleeson; A B Geert
Mutations in the FAD-binding fold of alcohol oxidase from Hansenula polymorpha.
Protein engineering 1991;4(7):821-9.