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Timothy Dafforn

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Mohd Firdaus Abdul-Wahab; Timothy R Dafforn; Takayuki Homma; Kazuhiro Nagata; Dee Olerenshaw; Michael Wright; Andrew D Miller
The pH sensitivity of murine heat shock protein 47 (HSP47) binding to collagen is affected by mutations in the breach histidine cluster.
Raúl Pacheco-Gómez; Alison Rodger; David I Roper; Corinne J I Smith; Stephen Addinall; Xi Cheng; Matthew R Hicks; Timothy R Dafforn
Tetramerization of ZapA is required for FtsZ bundling.
Matthew R Hicks; Søren V Hoffmann; Nykola C Jones; Yu-pin Lin; Alison Rodger; Timothy R Dafforn
Rapid injection linear dichroism for studying the kinetics of biological processes.

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2013: Mohd Firdaus Abdul-Wahab; Timothy R Dafforn; Takayuki Homma; Kazuhiro Nagata; Dee Olerenshaw; Michael Wright; Andrew D Miller
The pH sensitivity of murine heat shock protein 47 (HSP47) binding to collagen is affected by mutations in the breach histidine cluster.
The Journal of biological chemistry 2013;288(6):4452-61.
2013: Raúl Pacheco-Gómez; Alison Rodger; David I Roper; Corinne J I Smith; Stephen Addinall; Xi Cheng; Matthew R Hicks; Timothy R Dafforn
Tetramerization of ZapA is required for FtsZ bundling.
The Biochemical journal 2013;449(3):795-802.
2012: Matthew R Hicks; Søren V Hoffmann; Nykola C Jones; Yu-pin Lin; Alison Rodger; Timothy R Dafforn
Rapid injection linear dichroism for studying the kinetics of biological processes.
Analytical chemistry 2012;84(15):6561-6.
2012: Gillian L McNab; Elizabeth Sapey; Timothy R Dafforn; Alice Wood; Robert A Stockley
A novel model and molecular therapy for Z alpha-1 antitrypsin deficiency.
Mammalian genome : official journal of the International Mammalian Genome Society 2012;23(3-4):241-9.
2012: Daniel J Turner; Timothy R Dafforn; Ian Portman; Alison Rodger; David I Roper; Corinne J Smith; Matthew S Turner
The mechanics of FtsZ fibers.
Biophysical journal 2012;102(4):731-8.
2012: Raúl Pacheco-Gómez; Charles W Penn; Alison Rodger; Emma Stanley; Susan Stokoe; John Ward; Hannah J England; Matthew R Hicks; Julia Kraemer; Timothy R Dafforn
Detection of pathogenic bacteria using a homogeneous immunoassay based on shear alignment of virus particles and linear dichroism.
Analytical chemistry 2012;84(1):91-7.
2011: Vasilis Kosmoliaptsis; J Andrew Bradley; Afzal N Chaudhry; Timothy R Dafforn; David J Halsall; Craig J Taylor
High-resolution, three-dimensional modeling of human leukocyte antigen class I structure and surface electrostatic potential reveals the molecular basis for alloantibody binding epitopes.
Human immunology 2011;72(11):1049-59.
2011: Mohammed Jamshad; Yu-Pin Lin; Timothy J Knowles; Rosemary A Parslow; Craig Harris; Mark Wheatley; David R Poyner; Roslyn M Bill; Owen R T Thomas; Michael Overduin; Timothy R Dafforn
Surfactant-free purification of membrane proteins with intact native membrane environment.
Biochemical Society transactions 2011;39(3):813-8.
2011: Raúl Pacheco-Gómez; Timothy R Dafforn; David I Roper; Alison Rodger
The pH dependence of polymerization and bundling by the essential bacterial cytoskeletal protein FtsZ.
PloS one 2011;6(6):e19369.
2010: Abdenour Soufi; Anyaporn Sawasdichai; Anshuman Shukla; Peter Noy; Timothy R Dafforn; Corinne Smith; Padma-Sheela Jayaraman; Kevin Gaston
DNA compaction by the higher-order assembly of PRH/Hex homeodomain protein oligomers.
Nucleic acids research 2010;38(21):7513-25.
2010: Angeliki Damianoglou; Alison Rodger; Catherine Pridmore; Timothy R Dafforn; Jackie A Mosely; John M Sanderson; Matthew R Hicks
The synergistic action of melittin and phospholipase A2 with lipid membranes: development of linear dichroism for membrane-insertion kinetics.
Protein and peptide letters 2010;17(11):1351-62.
2010: Karen E Marshall; Matthew R Hicks; Thomas L Williams; Søren Vrønning Hoffmann; Alison Rodger; Timothy R Dafforn; Louise C Serpell
Characterizing the assembly of the Sup35 yeast prion fragment, GNNQQNY: structural changes accompany a fiber-to-crystal switch.
Biophysical journal 2010;98(2):330-8.
2009: Xinwang Cao; Unal Coskun; Manfred Rössle; Sabine B Buschhorn; Michal Grzybek; Timothy R Dafforn; Marc Lenoir; Michael Overduin; Kai Simons
Golgi protein FAPP2 tubulates membranes.
Proceedings of the National Academy of Sciences of the United States of America 2009;106(50):21121-5.
2009: Vasilis Kosmoliaptsis; Afzal N Chaudhry; Linda D Sharples; Halsall David; Timothy R Dafforn; J Andrew Bradley; Craig J Taylor
Predicting HLA class I alloantigen immunogenicity from the number and physiochemical properties of amino acid polymorphisms.
Transplantation 2009;88(6):791-8.
2009: Benjamin M Bulheller; Alison Rodger; Matthew R Hicks; Timothy R Dafforn; Louise C Serpell; Karen E Marshall; Elizabeth H C Bromley; Patrick J S King; Kevin J Channon; Derek N Woolfson; Jonathan D Hirst
Flow linear dichroism of some prototypical proteins.
Journal of the American Chemical Society 2009;131(37):13305-14.
2009: Matthew R Hicks; Timothy R Dafforn; Angeliki Damianoglou; Paul Wormell; Alison Rodger; Søren V Hoffmann
Synchrotron radiation linear dichroism spectroscopy of the antibiotic peptide gramicidin in lipid membranes.
The Analyst 2009;134(8):1623-8.
2009: Alexander J Lawson; Elizabeth A Walker; Scott A White; Timothy R Dafforn; Paul M Stewart; Jonathan P Ride
Mutations of key hydrophobic surface residues of 11 beta-hydroxysteroid dehydrogenase type 1 increase solubility and monodispersity in a bacterial expression system.
Protein science : a publication of the Protein Society 2009;18(7):1552-63.
2009: Timothy J Knowles; Rachael Finka; Corinne Smith; Yu-Pin Lin; Timothy R Dafforn; Michael Overduin
Membrane proteins solubilized intact in lipid containing nanoparticles bounded by styrene maleic acid copolymer.
Journal of the American Chemical Society 2009;131(22):7484-5.
2009: Sarah Nicholls; Karen P Piper; Fiyaz Mohammed; Timothy R Dafforn; Stefan Tenzer; Mahboob Salim; Premini Mahendra; Charles Craddock; Peter van Endert; Hansjörg Schild; Mark Cobbold; Victor H Engelhard; Paul A H Moss; Benjamin E Willcox
Secondary anchor polymorphism in the HA-1 minor histocompatibility antigen critically affects MHC stability and TCR recognition.
Proceedings of the National Academy of Sciences of the United States of America 2009;106(10):3889-94.
2009: Keiichiro Kami; Martyn Chidgey; Timothy R Dafforn; Michael Overduin
The desmoglein-specific cytoplasmic region is intrinsically disordered in solution and interacts with multiple desmosomal protein partners.
Journal of molecular biology 2009;386(2):531-43.
2009: Sarah M Batt; Lewis E H Bingle; Timothy R Dafforn; Christopher M Thomas
Bacterial genome partitioning: N-terminal domain of IncC protein encoded by broad-host-range plasmid RK2 modulates oligomerisation and DNA binding.
Journal of molecular biology 2009;385(5):1361-74.
2008: Matthew R Hicks; Angeliki Damianoglou; Alison Rodger; Timothy R Dafforn
Folding and membrane insertion of the pore-forming peptide gramicidin occur as a concerted process.
Journal of molecular biology 2008;383(2):358-66.
2008: Joanne Oates; Matthew Hicks; Timothy R Dafforn; Daniel DiMaio; Ann M Dixon
In vitro dimerization of the bovine papillomavirus E5 protein transmembrane domain.
Biochemistry 2008;47(34):8985-92.
2008: Matthew Conner; Matthew R Hicks; Timothy R Dafforn; Timothy J Knowles; Christian Ludwig; Susan Staddon; Michael Overduin; Ulrich L Günther; Johannes Thome; Mark Wheatley; David R Poyner; Alex C Conner
Functional and biophysical analysis of the C-terminus of the CGRP-receptor; a family B GPCR.
Biochemistry 2008;47(32):8434-44.
2008: Mohammed Jamshad; Sundaresan Rajesh; Zania Stamataki; Jane A McKeating; Timothy R Dafforn; Michael Overduin; Roslyn M Bill
Structural characterization of recombinant human CD81 produced in Pichia pastoris.
Protein expression and purification 2008;57(2):206-16.
2007: U Mirian Obiozo; T Harma C Brondijk; Andrew J White; Gijs I van Boxel; Timothy R Dafforn; Scott A White; J Baz Jackson
Substitution of tyrosine 146 in the dI component of proton-translocating transhydrogenase leads to reversible dissociation of the active dimer into inactive monomers.
The Journal of biological chemistry 2007;282(50):36434-43.
2007: Nigel C Brissett; Robert S Pitcher; Raquel Juarez; Angel J Picher; Andrew J Green; Timothy R Dafforn; Gavin C Fox; Luis Blanco; Aidan J Doherty
Structure of a NHEJ polymerase-mediated DNA synaptic complex.
Science (New York, N.Y.) 2007;318(5849):456-9.
2007: Elaine Small; Rachel Marrington; Alison Rodger; David Scott; Katherine Sloan; David I Roper; Timothy R Dafforn; Stephen G Addinall
FtsZ polymer-bundling by the Escherichia coli ZapA orthologue, YgfE, involves a conformational change in bound GTP.
Journal of molecular biology 2007;369(1):210-21.
2007: Tina Bhakta; Simon J Whitehead; John S Snaith; Timothy R Dafforn; John Wilkie; Sundaresan Rajesh; Scott A White; J Baz Jackson
Structures of the dI2dIII1 complex of proton-translocating transhydrogenase with bound, inactive analogues of NADH and NADPH reveal active site geometries.
Biochemistry 2007;46(11):3304-18.
2007: Timothy R Dafforn
So how do you know you have a macromolecular complex?
Acta crystallographica. Section D, Biological crystallography 2007;63(Pt 1):17-25.
2006: Yi-Pin Chang; Ravi Mahadeva; Wun-Shaing W Chang; Anshuman Shukla; Timothy R Dafforn; Yen-Ho Chu
Identification of a 4-mer peptide inhibitor that effectively blocks the polymerization of pathogenic Z alpha1-antitrypsin.
American journal of respiratory cell and molecular biology 2006;35(5):540-8.
2006: Andrew S Robertson; Didier Belorgey; David Gubb; Timothy R Dafforn; David A Lomas
Inhibitory activity of the Drosophila melanogaster serpin Necrotic is dependent on lysine residues in the D-helix.
The Journal of biological chemistry 2006;281(36):26437-43.
2006: Matthew R Hicks; Alison Rodger; Christopher M Thomas; Sarah M Batt; Timothy R Dafforn
Restriction enzyme kinetics monitored by UV linear dichroism.
Biochemistry 2006;45(29):8912-7.
2006: Alison Rodger; Rachel Marrington; Michael A Geeves; Matthew Hicks; Lahari de Alwis; Halsall David; Timothy R Dafforn
Looking at long molecules in solution: what happens when they are subjected to Couette flow?
Physical chemistry chemical physics : PCCP 2006;8(27):3161-71.
2006: Luke Alderwick; Virginie Molle; Laurent Kremer; Alain J Cozzone; Timothy R Dafforn; Gurdyal S Besra; Klaus Fütterer
Molecular structure of EmbR, a response element of Ser/Thr kinase signaling in Mycobacterium tuberculosis.
Proceedings of the National Academy of Sciences of the United States of America 2006;103(8):2558-63.
2006: Nadège Pelte; Andrew S Robertson; zhen zou; Didier Belorgey; Timothy R Dafforn; Haobo Jiang; David Lomas; Jean-Marc Reichhart; David Gubb
Immune challenge induces N-terminal cleavage of the Drosophila serpin Necrotic.
Insect biochemistry and molecular biology 2006;36(1):37-46.
2005: Rachel Marrington; Timothy R Dafforn; Halsall David; James I MacDonald; Matthew Hicks; Alison Rodger
Validation of new microvolume Couette flow linear dichroism cells.
The Analyst 2005;130(12):1608-16.
2005: Stephen G Addinall; Kenneth A Johnson; Timothy R Dafforn; Corinne Smith; Alison Rodger; Raul Paco Gomez; Katherine Sloan; Anne Blewett; David Scott; David I Roper
Expression, purification and crystallization of the cell-division protein YgfE from Escherichia coli.
Acta crystallographica. Section F, Structural biology and crystallization communications 2005;61(Pt 3):305-7.
2005: Thomas H Millard; Guillaume Bompard; Man Yeung Heung; Timothy R Dafforn; David Scott; Laura M Machesky; Klaus Fütterer
Structural basis of filopodia formation induced by the IRSp53/MIM homology domain of human IRSp53.
The EMBO journal 2005;24(2):240-50.
2004: Rachel Marrington; Elaine Small; Alison Rodger; Timothy R Dafforn; Stephen G Addinall
FtsZ fiber bundling is triggered by a conformational change in bound GTP.
The Journal of biological chemistry 2004;279(47):48821-9.
2004: Timothy R Dafforn; Corinne J I Smith
Natively unfolded domains in endocytosis: hooks, lines and linkers.
EMBO reports 2004;5(11):1046-52.
2004: Timothy R Dafforn; Alison Rodger
Linear dichroism of biomolecules: which way is up?
Current opinion in structural biology 2004;14(5):541-6.
2004: Rachel Marrington; Timothy R Dafforn; Halsall David; Alison Rodger
Micro-volume couette flow sample orientation for absorbance and fluorescence linear dichroism.
Biophysical journal 2004;87(3):2002-12.
2004: Helen Parfrey; Timothy R Dafforn; Didier Belorgey; David A Lomas; Ravi Mahadeva
Inhibiting polymerization: new therapeutic strategies for Z alpha1-antitrypsin-related emphysema.
American journal of respiratory cell and molecular biology 2004;31(2):133-9.
2004: Nicholas A Watkins; Timothy R Dafforn; M Kuijpers; C Brown; Babak Javid; Paul J Lehner; C Navarrete; Willem H Ouwehand
Molecular studies of anti-HLA-A2 using light-chain shuffling: a structural model for HLA antibody binding.
Tissue antigens 2004;63(4):345-54.
2004: Corinne J Smith; Timothy R Dafforn; Helen Kent; Catherine A Sims; Kavita Khubchandani-Aswani; Lin Zhang; Helen R Saibil; Barbara M F Pearse
Location of auxilin within a clathrin cage.
Journal of molecular biology 2004;336(2):461-71.
2004: Timothy R Dafforn; Robert N Pike; Stephen P Bottomley
Physical characterization of serpin conformations.
Methods (San Diego, Calif.) 2004;32(2):150-8.
2004: Peter A Smethurst; Lotta Joutsi-Korhonen; Marie N O'Connor; Erica Wilson; Nicola S Jennings; Stephen F Garner; Yanjun Zhang; C Graham Knight; Timothy R Dafforn; Ashley Buckle; Martin J W IJsseldijk; Philip G de Groot; Nicholas A Watkins; Richard W Farndale; Willem H Ouwehand
Identification of the primary collagen-binding surface on human glycoprotein VI by site-directed mutagenesis and by a blocking phage antibody.
Blood 2004;103(3):903-11.
2004: James A Glossop; Timothy R Dafforn; Stefan G E Roberts
A conformational change in TFIIB is required for activator-mediated assembly of the preinitiation complex.
Nucleic acids research 2004;32(5):1829-35.
2004: Timothy R Dafforn; Jascindra Rajendra; Halsall David; Louise C Serpell; Alison Rodger
Protein fiber linear dichroism for structure determination and kinetics in a low-volume, low-wavelength couette flow cell.
Biophysical journal 2004;86(1 Pt 1):404-10.
2003: Noelene S Quinsey; Hazel L Fitton; Paul B Coughlin; James C Whisstock; Timothy R Dafforn; Robin W Carrell; Stephen P Bottomley; Robert N Pike
Introduction of a mutation in the shutter region of antithrombin (Phe77 --> Leu) increases affinity for heparin and decreases thermal stability.
Biochemistry 2003;42(34):10169-73.
2003: Helen Parfrey; Ravi Mahadeva; Neil A Ravenhill; Aiwu Zhou; Timothy R Dafforn; Richard C Foreman; David A Lomas
Targeting a surface cavity of alpha 1-antitrypsin to prevent conformational disease.
The Journal of biological chemistry 2003;278(35):33060-6.
2003: Clare Green; Gemma Brown; Timothy R Dafforn; Jean-Marc Reichhart; Terri Morley; David A Lomas; David Gubb
Drosophila necrotic mutations mirror disease-associated variants of human serpins.
Development (Cambridge, England) 2003;130(7):1473-8.
2003: Emma C Morris; Timothy R Dafforn; Sharon L Forsyth; Melinda A Missen; Anita J Horvath; Lynne Hampson; Ian N Hampson; Graeme Currie; Robin W Carrell; Paul B Coughlin
Murine serpin 2A is a redox-sensitive intracellular protein.
The Biochemical journal 2003;371(Pt 1):165-73.
2003: Damian C Crowther; Louise C Serpell; Timothy R Dafforn; Bibek Gooptu; David A Lomas
Nucleation of alpha 1-antichymotrypsin polymerization.
Biochemistry 2003;42(8):2355-63.
2003: Andrew S Robertson; Didier Belorgey; Kathryn S Lilley; David A Lomas; David Gubb; Timothy R Dafforn
Characterization of the necrotic protein that regulates the Toll-mediated immune response in Drosophila.
The Journal of biological chemistry 2003;278(8):6175-80.
2002: Ravi Mahadeva; Timothy R Dafforn; Robin W Carrell; David A Lomas
6-mer peptide selectively anneals to a pathogenic serpin conformation and blocks polymerization. Implications for the prevention of Z alpha(1)-antitrypsin-related cirrhosis.
The Journal of biological chemistry 2002;277(9):6771-4.
2001: Timothy R Dafforn; Marina Della; A D Miller
The molecular interactions of heat shock protein 47 (Hsp47) and their implications for collagen biosynthesis.
The Journal of biological chemistry 2001;276(52):49310-9.
2001: Halsall David; A Rodger; Timothy R Dafforn
Linear dichroism for the detection of single base pair mutations.
Chemical communications (Cambridge, England) 2001;(23):2410-1.
2001: Aiwu Zhou; Richard Faint; Peter A Charlton; Timothy R Dafforn; Robin W Carrell; David A Lomas
Polymerization of plasminogen activator inhibitor-1.
The Journal of biological chemistry 2001;276(12):9115-22.
2001: AA Stoop; Eric Eldering; Timothy R Dafforn; Randy J Read; Hans Pannekoek
Different structural requirements for plasminogen activator inhibitor 1 (PAI-1) during latency transition and proteinase inhibition as evidenced by phage-displayed hypermutated PAI-1 libraries.
Journal of molecular biology 2001;305(4):773-83.
2000: Clare Green; Elena A Levashina; Carol McKimmie; Timothy R Dafforn; Jean-Marc Reichhart; David Gubb
The necrotic gene in Drosophila corresponds to one of a cluster of three serpin transcripts mapping at 43A1.2.
Genetics 2000;156(3):1117-27.
2000: Pasupathy Sivasothy; Timothy R Dafforn; Peter G W Gettins; David A Lomas
Pathogenic alpha 1-antitrypsin polymers are formed by reactive loop-beta-sheet A linkage.
The Journal of biological chemistry 2000;275(43):33663-8.
2000: PR Elliott; Xue Yuan Pei; Timothy R Dafforn; David A Lomas
Topography of a 2.0 A structure of alpha1-antitrypsin reveals targets for rational drug design to prevent conformational disease.
Protein science : a publication of the Protein Society 2000;9(7):1274-81.
2000: Klara J Belzar; Timothy R Dafforn; M Petitou; Robin W Carrell; James A Huntington
The effect of a reducing-end extension on pentasaccharide binding by antithrombin.
The Journal of biological chemistry 2000;275(12):8733-41.
2000: Aidan J Doherty; Timothy R Dafforn
Nick recognition by DNA ligases.
Journal of molecular biology 2000;296(1):43-56.
2000: Bibek Gooptu; Bart Hazes; Wun-Shaing W Chang; Timothy R Dafforn; Robin W Carrell; Randy J Read; David A Lomas
Inactive conformation of the serpin alpha(1)-antichymotrypsin indicates two-stage insertion of the reactive loop: implications for inhibitory function and conformational disease.
Proceedings of the National Academy of Sciences of the United States of America 2000;97(1):67-72.
1999: Timothy R Dafforn; Ravi Mahadeva; PR Elliott; Pasupathy Sivasothy; David A Lomas
A kinetic mechanism for the polymerization of alpha1-antitrypsin.
The Journal of biological chemistry 1999;274(14):9548-55.
1999: Ravi Mahadeva; Wun-Shaing W Chang; Timothy R Dafforn; D J Oakley; R C Foreman; J Calvin; D G Wight; David A Lomas
Heteropolymerization of S, I, and Z alpha1-antitrypsin and liver cirrhosis.
The Journal of clinical investigation 1999;103(7):999-1006.
1998: Nicholas J Beauchamp; Robert N Pike; M Daly; L Butler; Michael Makris; Timothy R Dafforn; A Zhou; Hazel L Fitton; F Eric Preston; Ian R Peake; Robin W Carrell
Antithrombins Wibble and Wobble (T85M/K): archetypal conformational diseases with in vivo latent-transition, thrombosis, and heparin activation.
Blood 1998;92(8):2696-706.
1998: Hazel L Fitton; Richard Skinner; Timothy R Dafforn; L Jin; Robert N Pike
The N-terminal segment of antithrombin acts as a steric gate for the binding of heparin.
Protein science : a publication of the Protein Society 1998;7(3):782-8.