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Seth Darst

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TOP 3
Albert Weixlbaumer; Robert Landick; Katherine Leon; Seth A Darst
Structural basis of transcriptional pausing in bacteria.
Joseph Osmundson; Lars F Westblade; Ann Hochschild; Cristina Montero-Diez; Seth A Darst
Promoter-specific transcription inhibition in Staphylococcus aureus by a phage protein.
Kelly-Anne Twist; Seyyed I Husnain; Josef D Franke; Deepti Jain; Elizabeth A Campbell; Bryce E Nickels; Mark S Thomas; Seth A Darst; Lars F Westblade
A novel method for the production of in vivo-assembled, recombinant Escherichia coli RNA polymerase lacking the α C-terminal domain.

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All Publications

2013: Albert Weixlbaumer; Robert Landick; Katherine Leon; Seth A Darst
Structural basis of transcriptional pausing in bacteria.
Cell 2013;152(3):431-41.
2012: Joseph Osmundson; Lars F Westblade; Ann Hochschild; Cristina Montero-Diez; Seth A Darst
Promoter-specific transcription inhibition in Staphylococcus aureus by a phage protein.
Cell 2012;151(5):1005-16.
2011: Kelly-Anne Twist; Seyyed I Husnain; Josef D Franke; Deepti Jain; Elizabeth A Campbell; Bryce E Nickels; Mark S Thomas; Seth A Darst; Lars F Westblade
A novel method for the production of in vivo-assembled, recombinant Escherichia coli RNA polymerase lacking the α C-terminal domain.
Protein science : a publication of the Protein Society 2011;20(6):986-95.
2011: Devendra B Srivastava; Seth A Darst
Derepression of bacterial transcription-repair coupling factor is associated with a profound conformational change.
Journal of molecular biology 2011;406(2):275-84.
2011: Zhanna Berdygulova; Lars F Westblade; Laurence Florens; Eugene V Koonin; Brian T Chait; Erlan Ramanculov; Michael Washburn; Seth A Darst; Konstantin Severinov; Leonid Minakhin
Temporal regulation of gene expression of the Thermus thermophilus bacteriophage P23-45.
Journal of molecular biology 2011;405(1):125-42.
2010: Matthew J Bick; Jacob J Banik; Seth A Darst; Sean F Brady
The 2.7 Å resolution structure of the glycopeptide sulfotransferase Teg14.
Acta crystallographica. Section D, Biological crystallography 2010;66(Pt 12):1278-86.
2010: Lars F Westblade; Elizabeth A Campbell; Chirangini Pukhrambam; Julio C Padovan; Bryce E Nickels; Valerie Lamour; Seth A Darst
Structural basis for the bacterial transcription-repair coupling factor/RNA polymerase interaction.
Nucleic acids research 2010;38(22):8357-69.
2010: Radhika Subramanian; Elizabeth M Wilson-Kubalek; Christopher P Arthur; Matthew J Bick; Elizabeth A Campbell; Seth A Darst; Ronald A Milligan; Tarun M Kapoor
Insights into antiparallel microtubule crosslinking by PRC1, a conserved nonmotor microtubule binding protein.
Cell 2010;142(3):433-43.
2010: Jie Chen; Seth A Darst; Devarajan Thirumalai
Promoter melting triggered by bacterial RNA polymerase occurs in three steps.
Proceedings of the National Academy of Sciences of the United States of America 2010;107(28):12523-8.
2010: Matthew J Bick; Jacob J Banik; Seth A Darst; Sean F Brady
Crystal structures of the glycopeptide sulfotransferase Teg12 in a complex with the teicoplanin aglycone.
Biochemistry 2010;49(19):4159-68.
2010: Julian Spagnuolo; Natacha Opalka; Wesley X Wen; Dragana Gagic; Elodie Chabaud; Pierdomenico Bellini; Matthew D Bennett; Gillian E Norris; Seth A Darst; Marjorie Russel; Jasna Rakonjac
Identification of the gate regions in the primary structure of the secretin pIV.
Molecular microbiology 2010;76(1):133-50.
2010: Pukhrambam Grihanjali Devi; Elizabeth A Campbell; Seth A Darst; Bryce E Nickels
Utilization of variably spaced promoter-like elements by the bacterial RNA polymerase holoenzyme during early elongation.
Molecular microbiology 2010;75(3):607-22.
2010: William J Lane; Seth A Darst
Molecular evolution of multisubunit RNA polymerases: sequence analysis.
Journal of molecular biology 2010;395(4):671-85.
2010: William J Lane; Seth A Darst
Molecular evolution of multisubunit RNA polymerases: structural analysis.
Journal of molecular biology 2010;395(4):686-704.
2010: Natacha Opalka; Jesse Brown; William J Lane; Kelly-Anne F Twist; Robert Landick; Francisco J Asturias; Seth A Darst
Complete structural model of Escherichia coli RNA polymerase from a hybrid approach.
PLoS biology 2010;8(9):.
2009: Valerie Lamour; Lars F Westblade; Elizabeth A Campbell; Seth A Darst
Crystal structure of the in vivo-assembled Bacillus subtilis Spx/RNA polymerase alpha subunit C-terminal domain complex.
Journal of structural biology 2009;168(2):352-6.
2009: Andrey Feklistov; Seth A Darst
Promoter recognition by bacterial alternative sigma factors: the price of high selectivity?
Genes & development 2009;23(20):2371-5.
2009: Matthew J Bick; Valérie Lamour; Kanagalaghatta R Rajashankar; Yuliya Gordiyenko; Carol V Robinson; Seth A Darst
How to switch off a histidine kinase: crystal structure of Geobacillus stearothermophilus KinB with the inhibitor Sda.
Journal of molecular biology 2009;386(1):163-77.
2008: Valérie Lamour; Steven T Rutherford; Konstantin Kuznedelov; Udupi A Ramagopal; Richard L Gourse; Konstantin Severinov; Seth A Darst
Crystal structure of Escherichia coli Rnk, a new RNA polymerase-interacting protein.
Journal of molecular biology 2008;383(2):367-79.
2008: Edmund C Schwartz; Alexander Shekhtman; Kaushik Dutta; Matthew R. Pratt; David Cowburn; Seth A Darst; Tom W Muir
A full-length group 1 bacterial sigma factor adopts a compact structure incompatible with DNA binding.
Chemistry & biology 2008;15(10):1091-103.
2008: Andrey Feklistov; Vladimir Mekler; Qiaorong Jiang; Lars F Westblade; Herbert Irschik; Rolf Jansen; Arkady Mustaev; Seth A Darst; Richard H. Ebright
Rifamycins do not function by allosteric modulation of binding of Mg2+ to the RNA polymerase active center.
Proceedings of the National Academy of Sciences of the United States of America 2008;105(39):14820-5.
2008: Elizabeth A Campbell; Lars F Westblade; Seth A Darst
Regulation of bacterial RNA polymerase sigma factor activity: a structural perspective.
Current opinion in microbiology 2008;11(2):121-7.
2008: Lars F Westblade; Leonid Minakhin; Konstantin Kuznedelov; Alan J Tackett; Emmanuel J Chang; Rachel A Mooney; Irina Vvedenskaya; Qing Jun Wang; David Fenyö; Michael P Rout; Robert Landick; Brian T Chait; Konstantin Severinov; Seth A Darst
Rapid isolation and identification of bacteriophage T4-encoded modifications of Escherichia coli RNA polymerase: a generic method to study bacteriophage/host interactions.
Journal of proteome research 2008;7(3):1244-50.
2007: Georgia A Patikoglou; Lars F Westblade; Elizabeth A Campbell; Valérie Lamour; William J Lane; Seth A Darst
Crystal structure of the Escherichia coli regulator of sigma70, Rsd, in complex with sigma70 domain 4.
Journal of molecular biology 2007;372(3):649-59.
2007: Elizabeth A Campbell; Roger Greenwell; Jennifer R Anthony; Sheng Wang; Lionel Lim; Kalyan Das; Heidi Sofia; Timothy J Donohue; Seth A Darst
A conserved structural module regulates transcriptional responses to diverse stress signals in bacteria.
Molecular cell 2007;27(5):793-805.
2007: Alexandra M Deaconescu; Nigel J Savery; Seth A Darst
The bacterial transcription repair coupling factor.
Current opinion in structural biology 2007;17(1):96-102.
2006: Margareta K Sorenson; Seth A Darst
Disulfide cross-linking indicates that FlgM-bound and free sigma28 adopt similar conformations.
Proceedings of the National Academy of Sciences of the United States of America 2006;103(45):16722-7.
2006: William J Lane; Seth A Darst
The structural basis for promoter -35 element recognition by the group IV sigma factors.
PLoS biology 2006;4(9):e269.
2006: Konstantin Kuznedelov; Valérie Lamour; Georgia A Patikoglou; Mark Chlenov; Seth A Darst; Konstantin Severinov
Recombinant Thermus aquaticus RNA polymerase for structural studies.
Journal of molecular biology 2006;359(1):110-21.
2006: Alexandra M Deaconescu; Anna L Chambers; Abigail Smith; Bryce E Nickels; Ann Hochschild; Nigel J Savery; Seth A Darst
Structural basis for bacterial transcription-coupled DNA repair.
Cell 2006;124(3):507-20.
2006: Valérie Lamour; Brian P Hogan; Dorothy A Erie; Seth A Darst
Crystal structure of Thermus aquaticus Gfh1, a Gre-factor paralog that inhibits rather than stimulates transcript cleavage.
Journal of molecular biology 2006;356(1):179-88.
2005: Alexandra M Deaconescu; Seth A Darst
Crystallization and preliminary structure determination of Escherichia coli Mfd, the transcription-repair coupling factor.
Acta crystallographica. Section F, Structural biology and crystallization communications 2005;61(Pt 12):1062-4.
2005: Rachel Anne Mooney; Seth A Darst; Robert Landick
Sigma and RNA polymerase: an on-again, off-again relationship?
Molecular cell 2005;20(3):335-45.
2005: Mark Chlenov; Shoko Masuda; Katsuhiko S Murakami; Vadim Nikiforov; Seth A Darst; Arkady Mustaev
Structure and function of lineage-specific sequence insertions in the bacterial RNA polymerase beta' subunit.
Journal of molecular biology 2005;353(1):138-54.
2005: Deepti Jain; Youngchang Kim; Karen L Maxwell; Steven Beasley; Rongguang Zhang; Gary N Gussin; Aled M Edwards; Seth A Darst
Crystal structure of bacteriophage lambda cII and its DNA complex.
Molecular cell 2005;19(2):259-69.
2005: Brian D Gregory; Bryce E Nickels; Seth A Darst; Ann Hochschild
An altered-specificity DNA-binding mutant of Escherichia coli sigma70 facilitates the analysis of sigma70 function in vivo.
Molecular microbiology 2005;56(5):1208-19.
2005: Elizabeth A Campbell; Olga Pavlova; Nikolay Zenkin; Fred Leon; Herbert Irschik; Rolf Jansen; Konstantin Severinov; Seth A Darst
Structural, functional, and genetic analysis of sorangicin inhibition of bacterial RNA polymerase.
The EMBO journal 2005;24(4):674-82.
2004: Shoko Masuda; Katsuhiko S Murakami; Sheng Wang; C Anders Olson; Jill R Donigian; Fred Leon; Seth A Darst; Elizabeth A Campbell
Crystal structures of the ADP and ATP bound forms of the Bacillus anti-sigma factor SpoIIAB in complex with the anti-anti-sigma SpoIIAA.
Journal of molecular biology 2004;340(5):941-56.
2004: Margareta K Sorenson; Soumya S Ray; Seth A Darst
Crystal structure of the flagellar sigma/anti-sigma complex sigma(28)/FlgM reveals an intact sigma factor in an inactive conformation.
Molecular cell 2004;14(1):127-38.
2004: Seth A Darst
New inhibitors targeting bacterial RNA polymerase.
Trends in biochemical sciences 2004;29(4):159-60.
2004: Catherine L Lawson; David Swigon; Katsuhiko S Murakami; Seth A Darst; Helen M Berman; Richard H. Ebright
Catabolite activator protein: DNA binding and transcription activation.
Current opinion in structural biology 2004;14(1):10-20.
2004: Deepti Jain; Bryce E Nickels; Li Sun; Ann Hochschild; Seth A Darst
Structure of a ternary transcription activation complex.
Molecular cell 2004;13(1):45-53.
2003: Kelly-Anne Wilson; Markus Kalkum; Jennifer Ottesen; Julia Yuzenkova; Brian T Chait; Robert Landick; Tom Muir; Konstantin Severinov; Seth A Darst
Structure of microcin J25, a peptide inhibitor of bacterial RNA polymerase, is a lassoed tail.
Journal of the American Chemical Society 2003;125(41):12475-83.
2003: Natacha Opalka; Mark Chlenov; Pablo Chacon; William J Rice; Willy Wriggers; Seth A Darst
Structure and function of the transcription elongation factor GreB bound to bacterial RNA polymerase.
Cell 2003;114(3):335-45.
2003: Simon L Dove; Seth A Darst; Ann Hochschild
Region 4 of sigma as a target for transcription regulation.
Molecular microbiology 2003;48(4):863-74.
2003: Elizabeth A Campbell; Jonathan L Tupy; Tanja M Gruber; Sheng Wang; Meghan M. Sharp; Carol A Gross; Seth A Darst
Crystal structure of Escherichia coli sigmaE with the cytoplasmic domain of its anti-sigma RseA.
Molecular cell 2003;11(4):1067-78.
2003: Katsuhiko S Murakami; Seth A Darst
Bacterial RNA polymerases: the wholo story.
Current opinion in structural biology 2003;13(1):31-9.
2003: Natacha Opalka; Roland Beckmann; Nicolas Boisset; Martha N Simon; Marjorie Russel; Seth A Darst
Structure of the filamentous phage pIV multimer by cryo-electron microscopy.
Journal of molecular biology 2003;325(3):461-70.
2003: Katsuhiko S Murakami; Shoko Masuda; Seth A Darst
Crystallographic analysis of Thermus aquaticus RNA polymerase holoenzyme and a holoenzyme/promoter DNA complex.
Methods in enzymology 2003;370():42-53.
2002: Bryce E Nickels; Simon L Dove; Katsuhiko S Murakami; Seth A Darst; Ann Hochschild
Protein-protein and protein-DNA interactions of sigma70 region 4 involved in transcription activation by lambdacI.
Journal of molecular biology 2002;324(1):17-34.
2002: Julio A Camarero; Alexander Shekhtman; Elizabeth A Campbell; Mark Chlenov; Tanja M Gruber; Donald A Bryant; Seth A Darst; David Cowburn; Tom W Muir
Autoregulation of a bacterial sigma factor explored by using segmental isotopic labeling and NMR.
Proceedings of the National Academy of Sciences of the United States of America 2002;99(13):8536-41.
2002: Katsuhiko S Murakami; Shoko Masuda; Elizabeth A Campbell; Oriana Muzzin; Seth A Darst
Structural basis of transcription initiation: an RNA polymerase holoenzyme-DNA complex.
Science (New York, N.Y.) 2002;296(5571):1285-90.
2002: Katsuhiko S Murakami; Shoko Masuda; Seth A Darst
Structural basis of transcription initiation: RNA polymerase holoenzyme at 4 A resolution.
Science (New York, N.Y.) 2002;296(5571):1280-4.
2002: Seth A Darst; Natacha Opalka; Pablo Chacon; Andrey Polyakov; Catherine Richter; Gongyi Zhang; Willy Wriggers
Conformational flexibility of bacterial RNA polymerase.
Proceedings of the National Academy of Sciences of the United States of America 2002;99(7):4296-301.
2002: Elizabeth A Campbell; Shoko Masuda; Jing L Sun; Oriana Muzzin; C Anders Olson; Sheng Wang; Seth A Darst
Crystal structure of the Bacillus stearothermophilus anti-sigma factor SpoIIAB with the sporulation sigma factor sigmaF.
Cell 2002;108(6):795-807.
2002: Elizabeth A Campbell; Oriana Muzzin; Mark Chlenov; Jing L Sun; C Anders Olson; Oren Weinman; Michelle L Trester-Zedlitz; Seth A Darst
Structure of the bacterial RNA polymerase promoter specificity sigma subunit.
Molecular cell 2002;9(3):527-39.
2001: Tatyana Naryshkina; Arkady Mustaev; Seth A Darst; Konstantin Severinov
The beta ' subunit of Escherichia coli RNA polymerase is not required for interaction with initiating nucleotide but is necessary for interaction with rifampicin.
The Journal of biological chemistry 2001;276(16):13308-13.
2001: Seth A Darst
Bacterial RNA polymerase.
Current opinion in structural biology 2001;11(2):155-62.
2001: Elizabeth A Campbell; Nataliya Korzheva; Arkady Mustaev; Katsuhiko S Murakami; S Nair; Alex Goldfarb; Seth A Darst
Structural mechanism for rifampicin inhibition of bacterial rna polymerase.
Cell 2001;104(6):901-12.
2001: Leonid Minakhin; S Bhagat; A Brunning; Elizabeth A Campbell; Seth A Darst; Richard H. Ebright; Konstantin Severinov
Bacterial RNA polymerase subunit omega and eukaryotic RNA polymerase subunit RPB6 are sequence, structural, and functional homologs and promote RNA polymerase assembly.
Proceedings of the National Academy of Sciences of the United States of America 2001;98(3):892-7.
2000: Nataliya Korzheva; Arkady Mustaev; Maxim Kozlov; Arun Malhotra; Vadim Nikiforov; Alex Goldfarb; Seth A Darst
A structural model of transcription elongation.
Science (New York, N.Y.) 2000;289(5479):619-25.
2000: Elizabeth A Campbell; Seth A Darst
The anti-sigma factor SpoIIAB forms a 2:1 complex with sigma(F), contacting multiple conserved regions of the sigma factor.
Journal of molecular biology 2000;300(1):17-28.
2000: D Kulish; Jookyung Lee; Ivan B Lomakin; B Nowicka; Asis K Das; Seth A Darst; K Normet; Sergei Borukhov
The functional role of basic patch, a structural element of Escherichia coli transcript cleavage factors GreA and GreB.
The Journal of biological chemistry 2000;275(17):12789-98.
2000: Natacha Opalka; R A Mooney; Catherine Richter; Konstantin Severinov; Robert Landick; Seth A Darst
Direct localization of a beta-subunit domain on the three-dimensional structure of Escherichia coli RNA polymerase.
Proceedings of the National Academy of Sciences of the United States of America 2000;97(2):617-22.
1999: G Zhang; Elizabeth A Campbell; Leonid Minakhin; C Richter; Konstantin Severinov; Seth A Darst
Crystal structure of Thermus aquaticus core RNA polymerase at 3.3 A resolution.
Cell 1999;98(6):811-24.
1999: N Loizos; Seth A Darst
Mapping interactions of Escherichia coli GreB with RNA polymerase and ternary elongation complexes.
The Journal of biological chemistry 1999;274(33):23378-86.
1998: Seth A Darst; Andrey Polyakov; Catherine Richter; Gongyi Zhang
Insights into Escherichia coli RNA polymerase structure from a combination of x-ray and electron crystallography.
Journal of structural biology 1998;124(2-3):115-22.
1998: Andrey Polyakov; Catherine Richter; Arun Malhotra; D Koulich; Sergei Borukhov; Seth A Darst
Visualization of the binding site for the transcript cleavage factor GreB on Escherichia coli RNA polymerase.
Journal of molecular biology 1998;281(3):465-73.
1998: G Zhang; Seth A Darst
Structure of the Escherichia coli RNA polymerase alpha subunit amino-terminal domain.
Science (New York, N.Y.) 1998;281(5374):262-6.
1998: Seth A Darst
A new twist on protein crystallization.
Proceedings of the National Academy of Sciences of the United States of America 1998;95(14):7848-9.
1998: N Loizos; Seth A Darst
Mapping protein-ligand interactions by footprinting, a radical idea.
Structure (London, England : 1993) 1998;6(6):691-5.
1998: Oriana Muzzin; Elizabeth A Campbell; L Xia; Elena Severinova; Seth A Darst; Konstantin Severinov
Disruption of Escherichia coli hepA, an RNA polymerase-associated protein, causes UV sensitivity.
The Journal of biological chemistry 1998;273(24):15157-61.
1998: Elena Severinova; Konstantin Severinov; Seth A Darst
Inhibition of Escherichia coli RNA polymerase by bacteriophage T4 AsiA.
Journal of molecular biology 1998;279(1):9-18.
1998: Seth A Darst; Andrey Polyakov; Catherine Richter; Gongyi Zhang
Structural studies of Escherichia coli RNA polymerase.
Cold Spring Harbor symposia on quantitative biology 1998;63():269-76.
1997: Konstantin Severinov; Seth A Darst
A mutant RNA polymerase that forms unusual open promoter complexes.
Proceedings of the National Academy of Sciences of the United States of America 1997;94(25):13481-6.
1997: Konstantin Severinov; R Mooney; Seth A Darst; Robert Landick
Tethering of the large subunits of Escherichia coli RNA polymerase.
The Journal of biological chemistry 1997;272(39):24137-40.
1997: Y Wang; Konstantin Severinov; N Loizos; David Fenyö; Ewa Heyduk; Tomasz Heyduk; Brian T Chait; Seth A Darst
Determinants for Escherichia coli RNA polymerase assembly within the beta subunit.
Journal of molecular biology 1997;270(5):648-62.
1997: D Koulich; M Orlova; Arun Malhotra; Andrej Sali; Seth A Darst; Sergei Borukhov
Domain organization of Escherichia coli transcript cleavage factors GreA and GreB.
The Journal of biological chemistry 1997;272(11):7201-10.
1996: Elena Severinova; Konstantin Severinov; David Fenyö; Michael T Marr; E N Brody; J W Roberts; Brian T Chait; Seth A Darst
Domain organization of the Escherichia coli RNA polymerase sigma 70 subunit.
Journal of molecular biology 1996;263(5):637-47.
1996: Konstantin Severinov; Arkady Mustaev; A Kukarin; O Muzzin; Irina Bass; Seth A Darst; Alex Goldfarb
Structural modules of the large subunits of RNA polymerase. Introducing archaebacterial and chloroplast split sites in the beta and beta' subunits of Escherichia coli RNA polymerase.
The Journal of biological chemistry 1996;271(44):27969-74.
1996: Arun Malhotra; Elena Severinova; Seth A Darst
Crystal structure of a sigma 70 subunit fragment from E. coli RNA polymerase.
Cell 1996;87(1):127-36.
1995: Konstantin Severinov; Arkady Mustaev; Elena Severinova; M Kozlov; Seth A Darst; Alex Goldfarb
The beta subunit Rif-cluster I is only angstroms away from the active center of Escherichia coli RNA polymerase.
The Journal of biological chemistry 1995;270(49):29428-32.
1995: Andrey Polyakov; Elena Severinova; Seth A Darst
Three-dimensional structure of E. coli core RNA polymerase: promoter binding and elongation conformations of the enzyme.
Cell 1995;83(3):365-73.
1995: Konstantin Severinov; D Markov; Elena Severinova; Vadim Nikiforov; R Landick; Seth A Darst; Alex Goldfarb
Streptolydigin-resistant mutants in an evolutionarily conserved region of the beta' subunit of Escherichia coli RNA polymerase.
The Journal of biological chemistry 1995;270(41):23926-9.
1995: Seth A Darst; A M Edwards
Epitaxial growth of protein crystals from two-dimensional crystals on lipid layers.
Current opinion in structural biology 1995;5(5):640-4.
1995: Konstantin Severinov; Arkady Mustaev; Elena Severinova; Irina Bass; Mikhail Kashlev; R Landick; Vadim Nikiforov; Alex Goldfarb; Seth A Darst
Assembly of functional Escherichia coli RNA polymerase containing beta subunit fragments.
Proceedings of the National Academy of Sciences of the United States of America 1995;92(10):4591-5.
1995: C E Stebbins; Sergei Borukhov; M Orlova; Andrey Polyakov; Alex Goldfarb; Seth A Darst
Crystal structure of the GreA transcript cleavage factor from Escherichia coli.
Nature 1995;373(6515):636-40.
1994: Seth A Darst; C E Stebbins; Sergei Borukhov; M Orlova; G Feng; R Landick; Alex Goldfarb
Crystallization of GreA, a transcript cleavage factor from Escherichia coli.
Journal of molecular biology 1994;242(4):582-5.
1994: Konstantin Severinov; David Fenyö; Elena Severinova; Arkady Mustaev; Brian T Chait; Alex Goldfarb; Seth A Darst
The sigma subunit conserved region 3 is part of "5'-face" of active center of Escherichia coli RNA polymerase.
The Journal of biological chemistry 1994;269(33):20826-8.