FIND

researchers

DISCOVER

relevant articles

PROMOTE

your research

now for free

Heini Dirr

Research Profile

Chemicals & Drugs

Concepts & Ideas

Physiology

Phenomena

Living Beings

Swine

Co-author Network

Publications

TOP 3
Bradley Peter; Sylvia Fanucchi; Nomxolisi Chloë Mina-Liz Ngubane; Heini W Dirr
Membrane mimetics induce helix formation and oligomerization of the chloride intracellular channel protein 1 transmembrane domain.
Gavin R Owen; Stoyan Stoychev; Ikechukwu Achilonu; Heini W Dirr
JNK1β1 is phosphorylated during expression in E. coli and in vitro by MKK4 at three identical novel sites.
Gavin R Owen; Ikechukwu Achilonu; Heini W Dirr
High yield purification of JNK1β1 and activation by in vitro reconstitution of the MEKK1→MKK4→JNK MAPK phosphorylation cascade.

Register for free to view:

Visualized networks:

See your personal network in
sophisticated graphical views

GeoTargeted Searches:

Locate experts around the world
and connect with global collaborators

Research Profiles:

See the research activity
of experts around the globe

Geonetwork of Heini Dirr (preview)

Cities where this author has publications
Cities where co-authors of this author have publications

Flash Player Required

All Publications

2013: Bradley Peter; Sylvia Fanucchi; Nomxolisi Chloë Mina-Liz Ngubane; Heini W Dirr
Membrane mimetics induce helix formation and oligomerization of the chloride intracellular channel protein 1 transmembrane domain.
Biochemistry 2013;52(16):2739-49.
2013: Gavin R Owen; Stoyan Stoychev; Ikechukwu Achilonu; Heini W Dirr
JNK1β1 is phosphorylated during expression in E. coli and in vitro by MKK4 at three identical novel sites.
Biochemical and biophysical research communications 2013;432(4):683-8.
2013: Gavin R Owen; Ikechukwu Achilonu; Heini W Dirr
High yield purification of JNK1β1 and activation by in vitro reconstitution of the MEKK1→MKK4→JNK MAPK phosphorylation cascade.
Protein expression and purification 2013;87(2):87-99.
2012: Derryn Legg-E'silva; Stoyan Stoychev; Ikechukwu Achilonu; Sylvia Fanucchi; Manuel Fernandes; Heini W Dirr
Role of arginine 29 and glutamic acid 81 interactions in the conformational stability of human chloride intracellular channel 1.
Biochemistry 2012;51(40):7854-62.
2012: Ikechukwu Achilonu; Megan Cross; Sylvia Fanucchi; Manuel Fernandes; Heini W Dirr
Role of individual histidines in the pH-dependent global stability of human chloride intracellular channel 1.
Biochemistry 2012;51(5):995-1004.
2011: Nishal Parbhoo; Stoyan H Stoychev; Ikechukwu Achilonu; Roslin J Adamson; Sylvia Fanucchi; Manuel Fernandes; Samantha Gildenhuys; Heini W Dirr
A conserved interdomain interaction is a determinant of folding cooperativity in the GST fold.
Biochemistry 2011;50(32):7067-75.
2011: David Balchin; Heini W Dirr; Yasien Sayed
Energetics of ligand binding to human glutathione transferase A1-1: Tyr-9 associated localisation of the C-terminal helix is ligand-dependent.
Biophysical chemistry 2011;156(2-3):153-8.
2010: David Balchin; Sylvia Fanucchi; Ikechukwu Achilonu; Roslin Adamson; Jonathan Burke; Manuel Fernandes; Samantha Gildenhuys; Heini W Dirr
Stability of the domain interface contributes towards the catalytic function at the H-site of class alpha glutathione transferase A1-1.
Biochimica et biophysica acta 2010;1804(12):2228-33.
2010: Ikechukwu Achilonu; Samantha Gildenhuys; Loren Fisher; Jonathan Burke; Sylvia Fanucchi; B Trevor Sewell; Manuel Fernandes; Heini W Dirr
The role of a topologically conserved isoleucine in glutathione transferase structure, stability and function.
Acta crystallographica. Section F, Structural biology and crystallization communications 2010;66(Pt 7):776-80.
2010: Samantha Gildenhuys; Louise Wallace; Jonathan P Burke; David Balchin; Yasien Sayed; Heini W Dirr
Class Pi glutathione transferase unfolds via a dimeric and not monomeric intermediate: functional implications for an unstable monomer.
Biochemistry 2010;49(24):5074-81.
2010: Samantha Gildenhuys; Marina Dobreva; Nichole Kinsley; Yasien Sayed; Jonathan Burke; Stephen Pelly; Graeme P Gordon; Muhammed Sayed; Trevor Sewell; Heini W Dirr
Arginine 15 stabilizes an S(N)Ar reaction transition state and the binding of anionic ligands at the active site of human glutathione transferase A1-1.
Biophysical chemistry 2010;146(2-3):118-25.
2009: Stoyan H Stoychev; Christos Nathaniel; Sylvia Fanucchi; Melissa Brock; Sheng Li; Kyle Asmus; Virgil L Woods; Heini W Dirr
Structural dynamics of soluble chloride intracellular channel protein CLIC1 examined by amide hydrogen-deuterium exchange mass spectrometry.
Biochemistry 2009;48(35):8413-21.
2008: Sylvia Fanucchi; Roslin Adamson; Heini W Dirr
Formation of an unfolding intermediate state of soluble chloride intracellular channel protein CLIC1 at acidic pH.
Biochemistry 2008;47(44):11674-81.
2008: Salerwe Mosebi; Lynn Morris; Heini W Dirr; Yasien Sayed
Active-site mutations in the South african human immunodeficiency virus type 1 subtype C protease have a significant impact on clinical inhibitor binding: kinetic and thermodynamic study.
Journal of virology 2008;82(22):11476-9.
2008: Samantha Gildenhuys; Louise Wallace; Heini W Dirr
Stability and unfolding of reduced Escherichia coli glutaredoxin 2: a monomeric structural homologue of the glutathione transferase family.
Biochemistry 2008;47(40):10801-8.
2008: Nichole Kinsley; Yasien Sayed; Salerwe Mosebi; Richard N Armstrong; Heini W Dirr
Characterization of the binding of 8-anilinonaphthalene sulfonate to rat class Mu GST M1-1.
Biophysical chemistry 2008;137(2-3):100-4.
2006: Lawrence C Thompson; John Walters; Jonathan Burke; James F Parsons; Richard N Armstrong; Heini W Dirr
Double mutation at the subunit interface of glutathione transferase rGSTM1-1 results in a stable, folded monomer.
Biochemistry 2006;45(7):2267-73.
2006: Carla S Alves; Diane C Kuhnert; Yasien Sayed; Heini W Dirr
The intersubunit lock-and-key motif in human glutathione transferase A1-1: role of the key residues Met51 and Phe52 in function and dimer stability.
The Biochemical journal 2006;393(Pt 2):523-8.
2005: Simona G Codreanu; Lawrence C Thompson; David L Hachey; Heini W Dirr; Richard N Armstrong
Influence of the dimer interface on glutathione transferase structure and dynamics revealed by amide H/D exchange mass spectrometry.
Biochemistry 2005;44(31):10605-12.
2005: Diane C Kuhnert; Yasien Sayed; Salerwe Mosebi; Muhammed Sayed; Trevor Sewell; Heini W Dirr
Tertiary interactions stabilise the C-terminal region of human glutathione transferase A1-1: a crystallographic and calorimetric study.
Journal of molecular biology 2005;349(4):825-38.
2005: Heini W Dirr; Tessa Little; Diane C Kuhnert; Yasien Sayed
A conserved N-capping motif contributes significantly to the stabilization and dynamics of the C-terminal region of class Alpha glutathione S-transferases.
The Journal of biological chemistry 2005;280(20):19480-7.
2004: Doris Kolobe; Yasien Sayed; Heini W Dirr
Characterization of bromosulphophthalein binding to human glutathione S-transferase A1-1: thermodynamics and inhibition kinetics.
The Biochemical journal 2004;382(Pt 2):703-9.
2003: Salerwe Mosebi; Yasien Sayed; Jonathan Burke; Heini W Dirr
Residue 219 impacts on the dynamics of the C-terminal region in glutathione transferase A1-1: implications for stability and catalytic and ligandin functions.
Biochemistry 2003;42(51):15326-32.
2003: Adrián Velázquez-Campoy; Sonia Vega; Erin Fleming; Usman Bacha; Yasien Sayed; Heini W Dirr; Ernesto Freire
Protease inhibition in African subtypes of HIV-1.
AIDS reviews 2003;5(3):165-71.
2003: Chris Nathaniel; Louise Wallace; Jonathan Burke; Heini W Dirr
The role of an evolutionarily conserved cis-proline in the thioredoxin-like domain of human class Alpha glutathione transferase A1-1.
The Biochemical journal 2003;372(Pt 1):241-6.
2002: Judith A T Hornby; Simona G Codreanu; Richard N Armstrong; Heini W Dirr
Molecular recognition at the dimer interface of a class mu glutathione transferase: role of a hydrophobic interaction motif in dimer stability and protein function.
Biochemistry 2002;41(48):14238-47.
2002: Jiann-Kae Luo; Judith A T Hornby; Louise Wallace; Jihong Chen; Richard N Armstrong; Heini W Dirr
Impact of domain interchange on conformational stability and equilibrium folding of chimeric class micro glutathione transferases.
Protein science : a publication of the Protein Society 2002;11(9):2208-17.
2002: Yasien Sayed; Judith A T Hornby; Marimar Lopez; Heini W Dirr
Thermodynamics of the ligandin function of human class Alpha glutathione transferase A1-1: energetics of organic anion ligand binding.
The Biochemical journal 2002;363(Pt 2):341-6.
2001: Jacqueline van der Spuy; Michael Cheetham; Heini W Dirr; Gregory L Blatch
The cochaperone murine stress-inducible protein 1: overexpression, purification, and characterization.
Protein expression and purification 2001;21(3):462-9.
2000: Victoria M Longshaw; Heini W Dirr; Gregory L Blatch; M Lässle
The in vitro phosphorylation of the co-chaperone mSTI1 by cell cycle kinases substantiates a predicted casein kinase II-p34cdc2-NLS (CcN) motif.
Biological chemistry 2000;381(11):1133-8.
2000: Judith A T Hornby; Jiann-Kae Luo; J M Stevens; Louise Wallace; W Kaplan; Richard N Armstrong; Heini W Dirr
Equilibrium folding of dimeric class mu glutathione transferases involves a stable monomeric intermediate.
Biochemistry 2000;39(40):12336-44.
2000: Fritha Hennessy; Michael Cheetham; Heini W Dirr; Gregory L Blatch
Analysis of the levels of conservation of the J domain among the various types of DnaJ-like proteins.
Cell stress & chaperones 2000;5(4):347-58.
2000: Louise Wallace; Jonathan Burke; Heini W Dirr
Domain-domain interface packing at conserved Trp-20 in class alpha glutathione transferase impacts on protein stability.
Biochimica et biophysica acta 2000;1478(2):325-32.
2000: J M Stevens; Richard N Armstrong; Heini W Dirr
Electrostatic interactions affecting the active site of class sigma glutathione S-transferase.
The Biochemical journal 2000;347 Pt 1():193-7.
2000: Jacqueline van der Spuy; B D Kana; Heini W Dirr; Gregory L Blatch
Heat shock cognate protein 70 chaperone-binding site in the co-chaperone murine stress-inducible protein 1 maps to within three consecutive tetratricopeptide repeat motifs.
The Biochemical journal 2000;345 Pt 3():645-51.
2000: Yasien Sayed; Louise Wallace; Heini W Dirr
The hydrophobic lock-and-key intersubunit motif of glutathione transferase A1-1: implications for catalysis, ligandin function and stability.
FEBS letters 2000;465(2-3):169-72.
1999: Louise Wallace; Heini W Dirr
Folding and assembly of dimeric human glutathione transferase A1-1.
Biochemistry 1999;38(50):16686-94.
1999: Heini W Dirr; Louise Wallace
Role of the C-terminal helix 9 in the stability and ligandin function of class alpha glutathione transferase A1-1.
Biochemistry 1999;38(47):15631-40.
1998: Louise Wallace; Gregory L Blatch; Heini W Dirr
A topologically conserved aliphatic residue in alpha-helix 6 stabilizes the hydrophobic core in domain II of glutathione transferases and is a structural determinant for the unfolding pathway.
The Biochemical journal 1998;336 ( Pt 2)():413-8.
1998: J M Stevens; Judith A T Hornby; Richard N Armstrong; Heini W Dirr
Class sigma glutathione transferase unfolds via a dimeric and a monomeric intermediate: impact of subunit interface on conformational stability in the superfamily.
Biochemistry 1998;37(44):15534-41.
1998: N Sluis-Cremer; L Wallace; J Burke; J Stevens; Heini W Dirr
Aflatoxin B1 and sulphobromophthalein binding to the dimeric human glutathione S-transferase A1-1: a fluorescence spectroscopic analysis.
European journal of biochemistry / FEBS 1998;257(2):434-42.
1998: Louise Wallace; N Sluis-Cremer; Heini W Dirr
Equilibrium and kinetic unfolding properties of dimeric human glutathione transferase A1-1.
Biochemistry 1998;37(15):5320-8.
1997: W Kaplan; P Hüsler; H Klump; J Erhardt; N Sluis-Cremer; Heini W Dirr
Conformational stability of pGEX-expressed Schistosoma japonicum glutathione S-transferase: a detoxification enzyme and fusion-protein affinity tag.
Protein science : a publication of the Protein Society 1997;6(2):399-406.
1996: N Sluis-Cremer; N Naidoo; Heini W Dirr
Class-pi glutathione S-transferase is unable to regain its native conformation after oxidative inactivation by hydrogen peroxide.
European journal of biochemistry / FEBS 1996;242(2):301-7.
1996: N Sluis-Cremer; N N Naidoo; W H Kaplan; T H Manoharan; W E Fahl; Heini W Dirr
Determination of a binding site for a non-substrate ligand in mammalian cytosolic glutathione S-transferases by means of fluorescence-resonance energy transfer.
European journal of biochemistry / FEBS 1996;241(2):484-8.
1996: J Erhardt; Heini W Dirr
Effect of glutathione, glutathione sulphonate and S-hexylglutathione on the conformational stability of class pi glutathione S-transferase.
FEBS letters 1996;391(3):313-6.
1995: N Sluis-Cremer; Heini W Dirr
Conformational stability of Cys45-alkylated and hydrogen peroxide-oxidised glutathione S-transferase.
FEBS letters 1995;371(2):94-8.
1995: J Erhardt; Heini W Dirr
Native dimer stabilizes the subunit tertiary structure of porcine class pi glutathione S-transferase.
European journal of biochemistry / FEBS 1995;230(2):614-20.
1995: P Bico; J Erhardt; W Kaplan; Heini W Dirr
Porcine class pi glutathione S-transferase: anionic ligand binding and conformational analysis.
Biochimica et biophysica acta 1995;1247(2):225-30.
1995: Peter Reinemer; Heini W Dirr; Robert Huber
X-ray structure methods for glutathione binding.
Methods in enzymology 1995;251():243-54.
1994: Heini W Dirr; Peter Reinemer; Robert Huber
Refined crystal structure of porcine class Pi glutathione S-transferase (pGST P1-1) at 2.1 A resolution.
Journal of molecular biology 1994;243(1):72-92.
1994: P Bico; C Y Chen; M Jones; J Erhardt; Heini W Dirr
Class pi glutathione S-transferase: Meisenheimer complex formation.
Biochemistry and molecular biology international 1994;33(5):887-92.
1994: Heini W Dirr; Peter Reinemer; Robert Huber
X-ray crystal structures of cytosolic glutathione S-transferases. Implications for protein architecture, substrate recognition and catalytic function.
European journal of biochemistry / FEBS 1994;220(3):645-61.
1993: I Sinning; Gerard J Kleywegt; S W Cowan; Peter Reinemer; Heini W Dirr; Robert Huber; Gary L Gilliland; R N Armstrong; X Ji; P G Board
Structure determination and refinement of human alpha class glutathione transferase A1-1, and a comparison with the Mu and Pi class enzymes.
Journal of molecular biology 1993;232(1):192-212.
1992: Peter Reinemer; Heini W Dirr; Rudolf Ladenstein; Robert Huber; M Lo Bello; G Federici; M W Parker
Three-dimensional structure of class pi glutathione S-transferase from human placenta in complex with S-hexylglutathione at 2.8 A resolution.
Journal of molecular biology 1992;227(1):214-26.
1992: T H Manoharan; Andrew Gulick; Peter Reinemer; Heini W Dirr; Robert Huber; William E Fahl
Mutational substitution of residues implicated by crystal structure in binding the substrate glutathione to human glutathione S-transferase pi.
Journal of molecular biology 1992;226(2):319-22.
1991: Heini W Dirr; Peter Reinemer
Equilibrium unfolding of class pi glutathione S-transferase.
Biochemical and biophysical research communications 1991;180(1):294-300.
1991: Peter Reinemer; Heini W Dirr; Rudolf Ladenstein; J Schäffer; O Gallay; Robert Huber
The three-dimensional structure of class pi glutathione S-transferase in complex with glutathione sulfonate at 2.3 A resolution.
The EMBO journal 1991;10(8):1997-2005.
1991: Heini W Dirr; Karlheinz Mann; Robert Huber; Rudolf Ladenstein; Peter Reinemer
Class pi glutathione S-transferase from pig lung. Purification, biochemical characterization, primary structure and crystallization.
European journal of biochemistry / FEBS 1991;196(3):693-8.
1988: Heini W Dirr; J C Schabort
Purification and partial characterization of the glutathione S-transferase of rat erythrocytes.
Biochimica et biophysica acta 1988;957(2):173-7.
1987: Heini W Dirr; J C Schabort
Glutathione S-transferase composition of rat erythrocytes.
Biochemistry international 1987;15(2):381-4.
1987: Heini W Dirr; J C Schabort
Characterization of the aflatoxin B1-binding site of rat albumin.
Biochimica et biophysica acta 1987;913(3):300-7.
1987: Heini W Dirr
Effects of hydrogen ion and fatty acid concentrations on the binding of aflatoxin B1 to human albumin.
Biochemistry international 1987;14(4):727-33.
1987: Heini W Dirr; J C Schabort
Intracellular aflatoxin B1-binding proteins in rat liver.
Biochemistry international 1987;14(2):297-302.
1987: Heini W Dirr
Solvent effects on the spectroscopic properties of aflatoxin B1.
The International journal of biochemistry 1987;19(11):1137-40.
1986: Heini W Dirr; J C Schabort
Aflatoxin B1 transport in rat blood plasma. Binding to albumin in vivo and in vitro and spectrofluorimetric studies into the nature of the interaction.
Biochimica et biophysica acta 1986;881(3):383-90.
1986: Heini W Dirr; J C Schabort; C Weitz
Cucurbitacin delta 23-reductase from the fruit of Cucurbita maxima var. Green Hubbard. Physicochemical and fluorescence properties and enzyme-ligand interactions.
The Biochemical journal 1986;233(3):649-53.