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Frédéric Ducancel

Research Profile

Genes & Molecular Sequences

Amino Acid Sequences

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Chemicals & Drugs

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Physiology

Mutagenesis, Site-Directed

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Publications

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Céline Ortega; André Pèlegrin; Sophie Richard; Narciso Costa; Jean-Yves Couraud; Frédéric Ducancel; Amaury Herbet; Nathalie Kersual; Isabelle Navarro-Teulon; Didier Boquet
High level prokaryotic expression of anti-Müllerian inhibiting substance type II receptor diabody, a new recombinant antibody for in vivo ovarian cancer imaging.
Bertrand Allard; Didier Boquet; Aurélie Borrull; Frédérique Deshayes; Frédéric Ducancel; Patricia Lamourette; Fabienne Priam; Anne Wijkhuisen; Jean-Yves Couraud
Generation and characterization of rendomab-B1, a monoclonal antibody displaying potent and specific antagonism of the human endothelin B receptor.
Jie-Xian Dong; Frédéric Ducancel; Hong-Tao Lei; Zhen-Feng Li; Yu-Dong Shen; Yuan-Ming Sun; Zhen-Lin Xu; Jin-Yi Yang; Jean-Claude Boulain; Hong Wang
Development of a single-chain variable fragment-alkaline phosphatase fusion protein and a sensitive direct competitive chemiluminescent enzyme immunoassay for detection of ractopamine in pork.

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All Publications

2013: Céline Ortega; André Pèlegrin; Sophie Richard; Narciso Costa; Jean-Yves Couraud; Frédéric Ducancel; Amaury Herbet; Nathalie Kersual; Isabelle Navarro-Teulon; Didier Boquet
High level prokaryotic expression of anti-Müllerian inhibiting substance type II receptor diabody, a new recombinant antibody for in vivo ovarian cancer imaging.
Journal of immunological methods 2013;387(1-2):11-20.
2013: Bertrand Allard; Didier Boquet; Aurélie Borrull; Frédérique Deshayes; Frédéric Ducancel; Patricia Lamourette; Fabienne Priam; Anne Wijkhuisen; Jean-Yves Couraud
Generation and characterization of rendomab-B1, a monoclonal antibody displaying potent and specific antagonism of the human endothelin B receptor.
mAbs 2013;5(1):56-69.
2012: Jie-Xian Dong; Frédéric Ducancel; Hong-Tao Lei; Zhen-Feng Li; Yu-Dong Shen; Yuan-Ming Sun; Zhen-Lin Xu; Jin-Yi Yang; Jean-Claude Boulain; Hong Wang
Development of a single-chain variable fragment-alkaline phosphatase fusion protein and a sensitive direct competitive chemiluminescent enzyme immunoassay for detection of ractopamine in pork.
Analytica chimica acta 2012;736():85-91.
2012: Frédéric Ducancel; Bruno H Muller
Molecular engineering of antibodies for therapeutic and diagnostic purposes.
mAbs 2012;4(4):445-57.
2012: Sophie Sibéril; Enrico A Stura; Dominique Bourel; christophe de romeuf; Frédéric Ducancel; Wolf-Herman Fridman; Sylvie Jorieux; Renée Ménez; Jean-Luc Teillaud
Effect of zinc on human IgG1 and its FcγR interactions.
Immunology letters 2012;143(1):60-9.
2012: Aude Violette; Daniel Biass; Frédéric Ducancel; sebastien dutertre; Igor Križaj; Adrijana Leonardi; Florian Noguier; David Piquemal; Reto Stöcklin; Yves Terrat; Philippe Favreau
Recruitment of glycosyl hydrolase proteins in a cone snail venomous arsenal: further insights into biomolecular features of Conus venoms.
Marine drugs 2012;10(2):258-80.
2012: Gilles Mourier; Adelajda Zorba; Fabrice Beau; Florence Cordier; Tolga Coskun; Muriel DELEPIERRE; Frédéric Ducancel; XingHuang Gao; Mariana Hajj; Amaury Herbet; Elodie Marcon; Denis Servent
Pharmacological and structural characterization of long-sarafotoxins, a new family of endothelin-like peptides: Role of the C-terminus extension.
Biochimie 2012;94(2):461-70.
2012: Mirian A F Hayashi; Frédéric Ducancel; Katsuhiro Konno
Natural Peptides with Potential Applications in Drug Development, Diagnosis, and/or Biotechnology.
International journal of peptides 2012;2012():757838.
2011: Enrico A Stura; Marc Bossus; Colette Jolivet-Reynaud; Sandrine Michel; Bruno H Muller; Frédéric Ducancel
Crystal structure of human prostate-specific antigen in a sandwich antibody complex.
Journal of molecular biology 2011;414(4):530-44.
2011: Bruno H Muller; Catherine Ott; Alain Ruffion; Alexandra Savatier; Enrico A Stura; Laurence Becquart; Marc Bossus; Narciso Costa; Guillaume L'Hostis; Sandrine Michel; Frédéric Ducancel
In vitro affinity maturation of an anti-PSA antibody for prostate cancer diagnostic assay.
Journal of molecular biology 2011;414(4):545-62.
2011: Bertrand Allard; Didier Boquet; Frédérique Deshayes; Frédéric Ducancel; Fabienne Priam; Anne Wijkhuisen; Jean-Yves Couraud
Electroporation-aided DNA immunization generates polyclonal antibodies against the native conformation of human endothelin B receptor.
DNA and cell biology 2011;30(9):727-37.
2008: Renée Ménez; Sandrine Michel; Bruno H Muller; Marc Bossus; Frédéric Ducancel; Colette Jolivet-Reynaud; Enrico A Stura
Crystal structure of a ternary complex between human prostate-specific antigen, its substrate acyl intermediate and an activating antibody.
Journal of molecular biology 2008;376(4):1021-33.
2006: Olivier Dubreuil; Bruno Muller; Marc Bossus; Alexandra Savatier; Frédéric Ducancel
[Directed molecular evolution of antibodies].
Journal de la Société de biologie 2006;200(4):355-63.
2005: Marc Graille; Enrico A Stura; Marc Bossus; Bruno H Muller; Odile Letourneur; Nicole Battail-Poirot; Geneviève Sibaï; Marie Gauthier; Dominique Rolland; Marie Hélène Le Du; Frédéric Ducancel
Crystal structure of the complex between the monomeric form of Toxoplasma gondii surface antigen 1 (SAG1) and a monoclonal antibody that mimics the human immune response.
Journal of molecular biology 2005;354(2):447-58.
2005: Loïc Quinton; Jean-Pierre Le Caer; Gilles Phan; Caroline Ligny-Lemaire; Julie Bourdais-Jomaron; Frédéric Ducancel; Julia Chamot-Rooke
Characterization of toxins within crude venoms by combined use of Fourier transform mass spectrometry and cloning.
Analytical chemistry 2005;77(20):6630-9.
2005: Olivier Dubreuil; Marc Bossus; Marc Graille; Maëlle Bilous; Alexandra Savatier; Michel Jolivet; André Ménez; Enrico A Stura; Frédéric Ducancel
Fine tuning of the specificity of an anti-progesterone antibody by first and second sphere residue engineering.
The Journal of biological chemistry 2005;280(26):24880-7.
2005: Lucía Chávez-Gutiérrez; Julie Bourdais; Gonzalo Aranda; Miguel Angel Vargas; Edna Matta-Camacho; Frédéric Ducancel; Lorenzo Segovia; Patricia Joseph-Bravo; Jean-Louis Charli
A truncated isoform of pyroglutamyl aminopeptidase II produced by exon extension has dominant-negative activity.
Journal of neurochemistry 2005;92(4):807-17.
2004: Mirian A F Hayashi; Caroline Ligny-Lemaire; Zvi Wollberg; Michaël Wery; Andrzej Galat; Tomohisa Ogawa; Bruno H Muller; Hung Lamthanh; Yvon Doljansky; Avner Bdolah; Reto Stöcklin; Frédéric Ducancel
Long-sarafotoxins: characterization of a new family of endothelin-like peptides.
Peptides 2004;25(8):1243-51.
2004: Jean-Claude Boulain; Frédéric Ducancel
Expression of recombinant alkaline phosphatase conjugates in Escherichia coli.
Methods in molecular biology (Clifton, N.J.) 2004;267():101-12.
2003: Diego Butera; Karen Skielka; Mary Ann McLane; Carrie Paquette-Straub; Frédéric Ducancel; Ana Maria Moura da Silva
Cloning, expression, and characterization of a bi-functional disintegrin/alkaline phosphatase hybrid protein.
Protein expression and purification 2003;31(2):286-91.
2003: Frédéric Ducancel
[Antibody diversity is endless!].
Médecine sciences : M/S 2003;19(8-9):790-2.
2003: Renée Ménez; Marc Bossus; Bruno H Muller; Geneviève Sibaï; Pascal Dalbon; Frédéric Ducancel; Colette Jolivet-Reynaud; Enrico A Stura
Crystal structure of a hydrophobic immunodominant antigenic site on hepatitis C virus core protein complexed to monoclonal antibody 19D9D6.
Journal of immunology (Baltimore, Md. : 1950) 2003;170(4):1917-24.
2002: Frédéric Ducancel
The sarafotoxins.
Toxicon : official journal of the International Society on Toxinology 2002;40(11):1541-5.
2002: Fabienne Lachaise; Fida Nassar; Frédéric Ducancel; Ruben Italiano; Ghislaine Martin
Characterization and tissue-specific expression of the Drosophila transaldolase gene.
Gene 2002;299(1-2):263-70.
2002: Balkiss Bouhaouala-Zahar; Rym Benkhalifa; Najet Srairi; Ilhem Zenouaki; Caroline Ligny-Lemaire; Pascal Drevet; François Sampieri; Marcel Pelhate; Mohamed El Ayeb; André Ménez; Habib Karoui; Frédéric Ducancel
A chimeric scorpion alpha-toxin displays de novo electrophysiological properties similar to those of alpha-like toxins.
European journal of biochemistry / FEBS 2002;269(12):2831-41.
2002: C Monnet; Florence Bettsworth; Enrico A Stura; Marie Hélène Le Du; Renée Ménez; L Derrien; Sophie Zinn-Justin; Bernard Gilquin; Geneviève Sibaï; Nicole Battail-Poirot; Michel Jolivet; André Ménez; M Arnaud; Frédéric Ducancel; Jean-Baptiste Charbonnier
Highly specific anti-estradiol antibodies: structural characterisation and binding diversity.
Journal of molecular biology 2002;315(4):699-712.
2001: Bruno H Muller; C Lamoure; Marie Hélène Le Du; L Cattolico; Evelyne Lajeunesse; F Lemaître; A Pearson; Frédéric Ducancel; André Ménez; Jean-Claude Boulain
Improving Escherichia coli alkaline phosphatase efficacy by additional mutations inside and outside the catalytic pocket.
Chembiochem : a European journal of chemical biology 2001;2(7-8):517-23.
2001: Florence Bettsworth; C Monnet; Benedicte Jardin-Watelet; Nicole Battail-Poirot; Bernard Gilquin; Michel Jolivet; André Menez; M Arnaud; Frédéric Ducancel
Functional characterization of two anti-estradiol antibodies as deduced from modelling and site-directed mutagenesis experiments.
Journal of molecular recognition : JMR 2001;14(2):99-109.
2000: N Germain; K Mérienne; Sophie Zinn-Justin; Jean-Claude Boulain; Frédéric Ducancel; André Ménez
Molecular and structural basis of the specificity of a neutralizing acetylcholine receptor-mimicking antibody, using combined mutational and molecular modeling analyses.
The Journal of biological chemistry 2000;275(28):21578-86.
2000: A Ricciardi; Marie Hélène Le Du; M Khayati; F Dajas; Jean-Claude Boulain; André Menez; Frédéric Ducancel
Do structural deviations between toxins adopting the same fold reflect functional differences?
The Journal of biological chemistry 2000;275(24):18302-10.
2000: Marie Hélène Le Du; A Ricciardi; M Khayati; Renée Ménez; Jean-Claude Boulain; André Ménez; Frédéric Ducancel
Stability of a structural scaffold upon activity transfer: X-ray structure of a three fingers chimeric protein.
Journal of molecular biology 2000;296(4):1017-26.
2000: Yoshiro Chuman; Ikuo Nobuhisa; Tomohisa Ogawa; Masanobu Deshimaru; Takahito Chijiwa; N H Tan; Yasuyuki Fukumaki; Yasuyuki Shimohigashi; Frédéric Ducancel; Jean-Claude Boulain; André Ménez; Motonori Ohno
Regional and accelerated molecular evolution in group I snake venom gland phospholipase A2 isozymes.
Toxicon : official journal of the International Society on Toxinology 2000;38(3):449-62.
1998: Michel Léonetti; Robert Thai; J Cotton; S Leroy; Pascal Drevet; Frédéric Ducancel; Jean-Claude Boulain; André Ménez
Increasing immunogenicity of antigens fused to Ig-binding proteins by cell surface targeting.
Journal of immunology (Baltimore, Md. : 1950) 1998;160(8):3820-7.
1998: K Lobeck; Pascal Drevet; Michel Léonetti; C Fromen-Romano; Frédéric Ducancel; Evelyne Lajeunesse; C Lemaire; André Ménez
Towards a recombinant vaccine against diphtheria toxin.
Infection and immunity 1998;66(2):418-23.
1998: Motonori Ohno; Renée Ménez; Tomohisa Ogawa; J M Danse; Yasuyuki Shimohigashi; C Fromen; Frédéric Ducancel; Sophie Zinn-Justin; Marie Hélène Le Du; Jean-Claude Boulain; T Tamiya; André Ménez
Molecular evolution of snake toxins: is the functional diversity of snake toxins associated with a mechanism of accelerated evolution?
Progress in nucleic acid research and molecular biology 1998;59():307-64.
1997: C Fromen-Romano; Bernard Maillère; Pascal Drevet; Evelyne Lajeunesse; Frédéric Ducancel; Jean-Claude Boulain; André Ménez
Transformation of a non-enzymatic toxin into a toxoid by genetic engineering.
Protein engineering 1997;10(10):1213-20.
1997: K Mérienne; N Germain; Sophie Zinn-Justin; Jean-Claude Boulain; Frédéric Ducancel; André Ménez
The functional architecture of an acetylcholine receptor-mimicking antibody.
The Journal of biological chemistry 1997;272(38):23775-83.
1997: Pascal Drevet; C Lemaire; Sylvaine Gasparini; Sophie Zinn-Justin; Evelyne Lajeunesse; Frédéric Ducancel; S Pinkasfeld; Marie Courçon; O Tremeau; Jean-Claude Boulain; André Ménez
High-level production and isotope labeling of snake neurotoxins, disulfide-rich proteins.
Protein expression and purification 1997;10(3):293-300.
1997: P Garnier; Frédéric Ducancel; T Ogawa; Jean-Claude Boulain; Françoise Goudey-Perrière; Claude Perrière; A Ménez
Complete amino-acid sequence of the beta-subunit of VTX from venom of the stonefish (Synanceia verrucosa) as identified from cDNA cloning experiments.
Biochimica et biophysica acta 1997;1337(1):1-5.
1996: Frédéric Ducancel; K Mérienne; C Fromen-Romano; O Trémeau; L Pillet; Pascal Drevet; Sophie Zinn-Justin; Jean-Claude Boulain; André Ménez
Mimicry between receptors and antibodies. Identification of snake toxin determinants recognized by the acetylcholine receptor and an acetylcholine receptor-mimicking monoclonal antibody.
The Journal of biological chemistry 1996;271(49):31345-53.
1996: B Bouhaouala-Zahar; Frédéric Ducancel; I Zenouaki; R Ben Khalifa; Lamia Borchani; Marcel Pelhate; Jean-Claude Boulain; Mohamed El Ayeb; A Ménez; Habib Karoui
A recombinant insect-specific alpha-toxin of Buthus occitanus tunetanus scorpion confers protection against homologous mammal toxins.
European journal of biochemistry / FEBS 1996;238(3):653-60.
1995: A Carrier; Frédéric Ducancel; N B Settiawan; L Cattolico; Bernard Maillère; Michel Léonetti; Pascal Drevet; André Ménez; Jean-Claude Boulain
Recombinant antibody-alkaline phosphatase conjugates for diagnosis of human IgGs: application to anti-HBsAg detection.
Journal of immunological methods 1995;181(2):177-86.
1995: O Trémeau; C Lemaire; Pascal Drevet; S Pinkasfeld; Frédéric Ducancel; Jean-Claude Boulain; André Ménez
Genetic engineering of snake toxins. The functional site of Erabutoxin a, as delineated by site-directed mutagenesis, includes variant residues.
The Journal of biological chemistry 1995;270(16):9362-9.
1994: J M Danse; Edward G Rowan; Sylvaine Gasparini; Frédéric Ducancel; H Vatanpour; Louise C Young; G Poorheidari; Evelyne Lajeunesse; Pascal Drevet; R Ménez
On the site by which alpha-dendrotoxin binds to voltage-dependent potassium channels: site-directed mutagenesis reveals that the lysine triplet 28-30 is not essential for binding.
FEBS letters 1994;356(2-3):153-8.
1994: Mohamed Amiche; Frédéric Ducancel; A Mor; Jean-Claude Boulain; A Menez; Pierre Nicolas
Precursors of vertebrate peptide antibiotics dermaseptin b and adenoregulin have extensive sequence identities with precursors of opioid peptides dermorphin, dermenkephalin, and deltorphins.
The Journal of biological chemistry 1994;269(27):17847-52.
1994: Sophie Zinn-Justin; L Pillet; Frédéric Ducancel; A Thomas; J C Smith; Jean-Claude Boulain; André Ménez
Engineering of protein epitopes: a single deletion in a snake toxin generates full binding capacity to a previously unrecognized antibody.
Protein engineering 1994;7(7):917-23.
1994: Eric Ezan; Frédéric Ducancel; Daniel Gillet; Pascal Drevet; André Ménez; JM Grognet; Jean-Claude Boulain
Recombinant technology in the preparation of immunogen and enzymatic tracer. Application to the development of an enzyme immunoassay for rat prolactin.
Journal of immunological methods 1994;169(2):205-11.
1993: Daniel Gillet; Eric Ezan; Frédéric Ducancel; C Gaillard; T Ardouin; M Istin; André Ménez; Jean-Claude Boulain; JM Grognet
Enzyme immunoassay using a rat prolactin-alkaline phosphatase recombinant tracer.
Analytical chemistry 1993;65(13):1779-84.
1993: Frédéric Ducancel; Daniel Gillet; A Carrier; Evelyne Lajeunesse; André Ménez; Jean-Claude Boulain
Recombinant colorimetric antibodies: construction and characterization of a bifunctional F(ab)2/alkaline phosphatase conjugate produced in Escherichia coli.
Bio/technology (Nature Publishing Company) 1993;11(5):601-5.
1993: Mohamed Amiche; Frédéric Ducancel; Evelyne Lajeunesse; Jean-Claude Boulain; A Ménez; Pierre Nicolas
Molecular cloning of a cDNA encoding the precursor of adenoregulin from frog skin. Relationships with the vertebrate defensive peptides, dermaseptins.
Biochemical and biophysical research communications 1993;191(3):983-90.
1993: D Hodgson; Sylvaine Gasparini; Pascal Drevet; Frédéric Ducancel; F Bouet; Jean-Claude Boulain; J B Harris; A Menez
Production of recombinant notechis 11'2L, an enzymatically active mutant of a phospholipase A2 from Notechis scutatus scutatus venom, as directly generated by cleavage of a fusion protein produced in Escherichia coli.
European journal of biochemistry / FEBS 1993;212(2):441-6.
1993: Frédéric Ducancel; V Matre; C Dupont; Evelyne Lajeunesse; Zvi Wollberg; A Bdolah; E Kochva; Jean-Claude Boulain; André Ménez
Cloning and sequence analysis of cDNAs encoding precursors of sarafotoxins. Evidence for an unusual "rosary-type" organization.
The Journal of biological chemistry 1993;268(5):3052-5.
1993: L Pillet; O Trémeau; Frédéric Ducancel; Pascal Drevet; Sophie Zinn-Justin; S Pinkasfeld; Jean-Claude Boulain; André Ménez
Genetic engineering of snake toxins. Role of invariant residues in the structural and functional properties of a curaremimetic toxin, as probed by site-directed mutagenesis.
The Journal of biological chemistry 1993;268(2):909-16.
1992: M Hervé; L Pillet; P Humbert; O Trémeau; Frédéric Ducancel; C Hirth; André Ménez
Role and environment of the conserved Lys27 of snake curaremimetic toxins as probed by chemical modifications, site-directed mutagenesis and photolabelling experiments.
European journal of biochemistry / FEBS 1992;208(1):125-31.
1992: Daniel Gillet; Frédéric Ducancel; E Pradel; Michel Léonetti; André Ménez; Jean-Claude Boulain
Insertion of a disulfide-containing neurotoxin into E. coli alkaline phosphatase: the hybrid retains both biological activities.
Protein engineering 1992;5(3):273-8.
1992: Frédéric Ducancel; Daniel Gillet; André Ménez; Jean-Claude Boulain
[Recombinant colorimetric antibodies: genetic construction and production in E. coli].
Comptes rendus de l'Académie des sciences. Série III, Sciences de la vie 1992;315(6):221-4.
1991: Frédéric Ducancel; Edward G Rowan; E Cassar; A L Harvey; A Ménez; Jean-Claude Boulain
Amino acid sequence of a muscarinic toxin deduced from the cDNA nucleotide sequence.
Toxicon : official journal of the International Society on Toxinology 1991;29(4-5):516-20.
1990: P Boyot; L Pillet; Frédéric Ducancel; Jean-Claude Boulain; O Tremeau; André Ménez
A recombinant snake neurotoxin generated by chemical cleavage of a hybrid protein recovers full biological properties.
FEBS letters 1990;266(1-2):87-90.
1990: Edward G Rowan; Frédéric Ducancel; Y Doljansky; Alan L Harvey; Jean-Claude Boulain; André Ménez
Nucleotide sequence encoding a 'synergistic-like' protein from the venom glands of Dendroaspis angusticeps.
Nucleic acids research 1990;18(6):1639.
1990: Frédéric Ducancel; G Guignery-Frelat; Jean-Claude Boulain; André Ménez
Nucleotide sequence and structure analysis of cDNAs encoding short-chain neurotoxins from venom glands of a sea snake (Aipysurus laevis).
Toxicon : official journal of the International Society on Toxinology 1990;28(1):119-23.
1989: Frédéric Ducancel; Jean-Claude Boulain; O Trémeau; André Ménez
Direct expression in E. coli of a functionally active protein A--snake toxin fusion protein.
Protein engineering 1989;3(2):139-43.
1988: C Bouchier; Frédéric Ducancel; G Guignery-Frelat; C Bon; Jean-Claude Boulain; André Ménez
Cloning and sequencing of cDNAs encoding the two subunits of Crotoxin.
Nucleic acids research 1988;16(18):9050.
1988: Frédéric Ducancel; G Guignery-Frelat; C Bouchier; André Ménez; Jean-Claude Boulain
Complete amino acid sequence of a PLA2 from the tiger snake Notechis scutatus scutatus as deduced from a complementary DNA.
Nucleic acids research 1988;16(18):9049.
1988: Frédéric Ducancel; G Guignery-Frelat; C Bouchier; André Ménez; Jean-Claude Boulain
Sequence analysis of a cDNA encoding a PLA2 from the sea-snake Aipysurus laevis.
Nucleic acids research 1988;16(18):9048.
1987: G Guignery-Frelat; Frédéric Ducancel; André Ménez; Jean-Claude Boulain
Sequence of a cDNA encoding a snake venom phospholipase A2.
Nucleic acids research 1987;15(14):5892.