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Raafat El-Maghrabi

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Song-Gun Kim; Michael Cavalier; M Raafat El-Maghrabi; Yong-Hwan Lee
A direct substrate-substrate interaction found in the kinase domain of the bifunctional enzyme, 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase.
Perumal Ramasamy; M Raafat El-Maghrabi; Gary Halada; Lisa M Miller; Miriam H Rafailovich
Examination of interactions of oppositely charged proteins in gels.
Song-Gun Kim; Nathan P Manes; M Raafat El-Maghrabi; Yong-Hwan Lee
Crystal structure of the hypoxia-inducible form of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFKFB3): a possible new target for cancer therapy.

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2007: Song-Gun Kim; Michael Cavalier; M Raafat El-Maghrabi; Yong-Hwan Lee
A direct substrate-substrate interaction found in the kinase domain of the bifunctional enzyme, 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase.
Journal of molecular biology 2007;370(1):14-26.
2007: Perumal Ramasamy; M Raafat El-Maghrabi; Gary Halada; Lisa M Miller; Miriam H Rafailovich
Examination of interactions of oppositely charged proteins in gels.
Langmuir : the ACS journal of surfaces and colloids 2007;23(4):2021-9.
2006: Song-Gun Kim; Nathan P Manes; M Raafat El-Maghrabi; Yong-Hwan Lee
Crystal structure of the hypoxia-inducible form of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFKFB3): a possible new target for cancer therapy.
The Journal of biological chemistry 2006;281(5):2939-44.
2005: Subir R Maitra; Sikha Bhaduri; M Raafat El-Maghrabi; Marc J Shapiro
Inhibition of matrix metalloproteinase on hepatic transforming growth factor beta1 and caspase-3 activation in hemorrhage.
Academic emergency medicine : official journal of the Society for Academic Emergency Medicine 2005;12(9):797-803.
2005: Subir R Maitra; Marc J Shapiro; Sikha Bhaduri; M Raafat El-Maghrabi
Effect of chemically modified tetracycline on transforming growth factor-beta1 and caspase-3 activation in liver of septic rats.
Critical care medicine 2005;33(7):1577-81.
2005: Nathan P Manes; M Raafat El-Maghrabi
The kinase activity of human brain 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase is regulated via inhibition by phosphoenolpyruvate.
Archives of biochemistry and biophysics 2005;438(2):125-36.
2003: Subir R Maitra; Elliott Chen; Dominik Rosa; Patrick D Valane; M Raafat El-Maghrabi; Collin E M Brathwaite
Modulations of signal transduction pathways during sepsis and the effects of insulin and mifepristone.
Academic emergency medicine : official journal of the Society for Academic Emergency Medicine 2003;10(1):1-8.
2000: Subir R Maitra; S Wang; M Raafat El-Maghrabi
Effects of RU486 on Glu-6-pase gene expression in hemorrhage and resuscitation.
Shock (Augusta, Ga.) 2000;14(5):578-81.
2000: Subir R Maitra; S Wang; M Raafat El-Maghrabi; Mark C Henry
Regulation of liver and kidney glucose-6-phosphatase gene expression in hemorrhage and resuscitation.
Academic emergency medicine : official journal of the Society for Academic Emergency Medicine 2000;7(7):731-8.
2000: Subir R Maitra; S Wang; Collin E M Brathwaite; M Raafat El-Maghrabi
Alterations in glucose-6-phosphatase gene expression in sepsis.
The Journal of trauma 2000;49(1):38-42.
1999: Subir R Maitra; M L Gestring; M Raafat El-Maghrabi; C H Lang; Mark C Henry
Endotoxin-induced alterations in hepatic glucose-6-phosphatase activity and gene expression.
Molecular and cellular biochemistry 1999;196(1-2):79-83.
1997: Subir R Maitra; M Gestring; M Raafat El-Maghrabi
Alterations in hepatic 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase and glucose-6-phosphatase gene expression after hemorrhagic hypotension and resuscitation.
Shock (Augusta, Ga.) 1997;8(5):385-8.
1995: D Argaud; A J Lange; T C Becker; David A Okar; M Raafat El-Maghrabi; Christopher B Newgard; S J Pilkis
Adenovirus-mediated overexpression of liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase in gluconeogenic rat hepatoma cells. Paradoxical effect on Fru-2,6-P2 levels.
The Journal of biological chemistry 1995;270(41):24229-36.
1995: M Raafat El-Maghrabi; A J Lange; W Jiang; K Yamagata; M Stoffel; J Takeda; A A Fernald; Michelle M Le Beau; Graeme I Bell; L Baker
Human fructose-1,6-bisphosphatase gene (FBP1): exon-intron organization, localization to chromosome bands 9q22.2-q22.3, and mutation screening in subjects with fructose-1,6-bisphosphatase deficiency.
Genomics 1995;27(3):520-5.
1994: Subir R Maitra; W Pan; A J Lange; M Raafat El-Maghrabi; Naji N Abumrad; S J Pilkis
Glucose-6-phosphatase gene expression and activity are modulated in hemorrhagic shock: evidence for a new heat-sensitive activator.
Biochemical and biophysical research communications 1994;204(2):716-24.
1994: A J Lange; D Argaud; M Raafat El-Maghrabi; W Pan; Subir R Maitra; S J Pilkis
Isolation of a cDNA for the catalytic subunit of rat liver glucose-6-phosphatase: regulation of gene expression in FAO hepatoma cells by insulin, dexamethasone and cAMP.
Biochemical and biophysical research communications 1994;201(1):302-9.
1993: Irwin Kurland; M Raafat El-Maghrabi; S J Pilkis
Lack of evidence for a role of Cys-138 as a base catalyst in the skeletal muscle 6-phosphofructo-2-kinase reaction.
Biochemical and biophysical research communications 1993;195(1):229-36.
1993: Nada A Abumrad; M Raafat El-Maghrabi; Ez-Zoubir Amri; E Lopez; Paul A Grimaldi
Cloning of a rat adipocyte membrane protein implicated in binding or transport of long-chain fatty acids that is induced during preadipocyte differentiation. Homology with human CD36.
The Journal of biological chemistry 1993;268(24):17665-8.
1993: Irwin Kurland; L Li; A J Lange; J J Correia; M Raafat El-Maghrabi; S J Pilkis
Regulation of rat 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase. Role of the NH2-terminal region.
The Journal of biological chemistry 1993;268(19):14056-64.
1993: C Espinet; A M Vargas; M Raafat El-Maghrabi; A J Lange; S J Pilkis
Expression of the liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase mRNA in FAO-1 cells.
The Biochemical journal 1993;293 ( Pt 1)():173-9.
1993: M Raafat El-Maghrabi; M Gidh-Jain; L R Austin; S J Pilkis
Isolation of a human liver fructose-1,6-bisphosphatase cDNA and expression of the protein in Escherichia coli. Role of ASP-118 and ASP-121 in catalysis.
The Journal of biological chemistry 1993;268(13):9466-72.
1992: A J Lange; C Espinet; R Hall; M Raafat El-Maghrabi; A M Vargas; R J Miksicek; Daryl K Granner; S J Pilkis
Regulation of gene expression of rat skeletal muscle/liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase. Isolation and characterization of a glucocorticoid response element in the first intron of the gene.
The Journal of biological chemistry 1992;267(22):15673-80.
1992: M Raafat El-Maghrabi; L R Austin; J J Correia; S J Pilkis
Lysine 274 is essential for fructose 2,6-bisphosphate inhibition of fructose-1,6-bisphosphatase.
The Journal of biological chemistry 1992;267(10):6526-30.
1992: Irwin Kurland; M Raafat El-Maghrabi; J J Correia; S J Pilkis
Rat liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase. Properties of phospho- and dephospho- forms and of two mutants in which Ser32 has been changed by site-directed mutagenesis.
The Journal of biological chemistry 1992;267(7):4416-23.
1991: A J Lange; M Raafat El-Maghrabi; S J Pilkis
Isolation of bovine liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase cDNA: bovine liver and heart forms of the enzyme are separate gene products.
Archives of biochemistry and biophysics 1991;290(1):258-63.
1991: M Raafat El-Maghrabi; S J Pilkis
Expression of rat liver fructose-1,6-bisphosphatase in Escherichia coli.
Biochemical and biophysical research communications 1991;176(1):137-44.
1991: M Raafat El-Maghrabi; A J Lange; L Kümmel; S J Pilkis
The rat fructose-1,6-bisphosphatase gene. Structure and regulation of expression.
The Journal of biological chemistry 1991;266(4):2115-20.
1990: K Lin; Irwin Kurland; L Z Xu; A J Lange; J Pilkis; M Raafat El-Maghrabi; S J Pilkis
Expression of mammalian liver glycolytic/gluconeogenic enzymes in Escherichia coli: recovery of active enzyme is strain and temperature dependent.
Protein expression and purification 1990;1(2):169-76.
1990: S J Pilkis; M Raafat El-Maghrabi; T H Claus
Fructose-2,6-bisphosphate in control of hepatic gluconeogenesis. From metabolites to molecular genetics.
Diabetes care 1990;13(6):582-99.
1989: C T Chien; Albert Tauler; A J Lange; K Chan; R L Printz; M Raafat El-Maghrabi; Daryl K Granner; S J Pilkis
Expression of rat hepatic glucokinase in Escherichia coli.
Biochemical and biophysical research communications 1989;165(2):817-25.
1989: Albert Tauler; A J Lange; M Raafat El-Maghrabi; S J Pilkis
Expression of rat liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase and its kinase domain in Escherichia coli.
Proceedings of the National Academy of Sciences of the United States of America 1989;86(19):7316-20.
1989: A J Lange; L Kummel; M Raafat El-Maghrabi; Albert Tauler; A Colosia; A Marker; S J Pilkis
Sequence of the 5'-flanking region of the rat 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase gene: regulation by glucocorticoids.
Biochemical and biophysical research communications 1989;162(2):753-60.
1989: A J Marker; A D Colosia; Albert Tauler; D H Solomon; Y Cayre; A J Lange; M Raafat El-Maghrabi; S J Pilkis
Glucocorticoid regulation of hepatic 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase gene expression.
The Journal of biological chemistry 1989;264(12):7000-4.
1988: A D Colosia; A J Marker; A J Lange; M Raafat El-Maghrabi; Daryl K Granner; Albert Tauler; J Pilkis; S J Pilkis
Induction of rat liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase mRNA by refeeding and insulin.
The Journal of biological chemistry 1988;263(35):18669-77.
1988: Paul Kountz; S Freeman; A G Cook; M Raafat El-Maghrabi; J R Knowles; S J Pilkis
The stereochemical course of phospho group transfer catalyzed by rat liver 6-phosphofructo-2-kinase.
The Journal of biological chemistry 1988;263(31):16069-72.
1988: M Raafat El-Maghrabi; J Pilkis; A J Marker; A D Colosia; G D'Angelo; B A Fraser; S J Pilkis
cDNA sequence of rat liver fructose-1,6-bisphosphatase and evidence for down-regulation of its mRNA by insulin.
Proceedings of the National Academy of Sciences of the United States of America 1988;85(22):8430-4.
1988: Mark Lively; M Raafat El-Maghrabi; J Pilkis; G D'Angelo; A D Colosia; J A Ciavola; B A Fraser; S J Pilkis
Complete amino acid sequence of rat liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase.
The Journal of biological chemistry 1988;263(2):839-49.
1988: S J Pilkis; M Raafat El-Maghrabi; T H Claus
Hormonal regulation of hepatic gluconeogenesis and glycolysis.
Annual review of biochemistry 1988;57():755-83.
1988: S J Pilkis; T H Claus; M Raafat El-Maghrabi
The role of cyclic AMP in rapid and long-term regulation of gluconeogenesis and glycolysis.
Advances in second messenger and phosphoprotein research 1988;22():175-91.
1987: Albert Tauler; M Raafat El-Maghrabi; S J Pilkis
Functional homology of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase, phosphoglycerate mutase, and 2,3-bisphosphoglycerate mutase.
The Journal of biological chemistry 1987;262(35):16808-15.
1987: S J Pilkis; Mark Lively; M Raafat El-Maghrabi
Active site sequence of hepatic fructose-2,6-bisphosphatase. Homology in primary structure with phosphoglycerate mutase.
The Journal of biological chemistry 1987;262(26):12672-5.
1987: M Raafat El-Maghrabi; T M Pate; G D'Angelo; J J Correia; Mark Lively; S J Pilkis
Rat liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase. Identification of essential sulfhydryl residues in the primary sequence of the enzyme.
The Journal of biological chemistry 1987;262(24):11714-20.
1987: A D Colosia; Mark Lively; M Raafat El-Maghrabi; S J Pilkis
Isolation of a cDNA clone for rat liver 6-phosphofructo 2-kinase/fructose 2,6-bisphosphatase.
Biochemical and biophysical research communications 1987;143(3):1092-8.
1986: Paul Kountz; R W McCain; M Raafat El-Maghrabi; S J Pilkis
Hepatic 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase: phosphate dependence and effects of other oxyanions.
Archives of biochemistry and biophysics 1986;251(1):104-13.
1986: S J Pilkis; M M McGrane; Paul Kountz; M Raafat El-Maghrabi; J Pilkis; Bruce E Maryanoff; Allen B Reitz; S J Benkovic
The effect of arabinose 1,5-bisphosphate on rat hepatic 6-phosphofructo-1-kinase and fructose-1,6-bisphosphatase.
Biochemical and biophysical research communications 1986;138(1):159-66.
1986: H B Stewart; M Raafat El-Maghrabi; S J Pilkis
Mechanism of activation of fructose-2,6-bisphosphatase by cAMP-dependent protein kinase.
The Journal of biological chemistry 1986;261(19):8793-8.
1986: M Raafat El-Maghrabi; J J Correia; P J Heil; T M Pate; C E Cobb; S J Pilkis
Tissue distribution, immunoreactivity, and physical properties of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase.
Proceedings of the National Academy of Sciences of the United States of America 1986;83(14):5005-9.
1986: S J Pilkis; E Fox; L Wolfe; L Rothbarth; A Colosia; H B Stewart; M Raafat El-Maghrabi
Hormonal modulation of key hepatic regulatory enzymes in the gluconeogenic/glycolytic pathway.
Annals of the New York Academy of Sciences 1986;478():1-19.
1985: F Nyfeler; M Raafat El-Maghrabi; S J Pilkis
Effect of pentobarbital on fructose 2,6-bisphosphate metabolism in isolated rat hepatocytes.
The American journal of physiology 1985;249(5 Pt 1):E525-33.
1985: H B Stewart; M Raafat El-Maghrabi; S J Pilkis
Evidence for a phosphoenzyme intermediate in the reaction pathway of rat hepatic fructose-2,6-bisphosphatase.
The Journal of biological chemistry 1985;260(24):12935-41.
1985: S J Pilkis; J Pilkis; M Raafat El-Maghrabi; T H Claus
The sugar phosphate specificity of rat hepatic 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase.
The Journal of biological chemistry 1985;260(12):7551-6.
1985: Paul Kountz; M Raafat El-Maghrabi; S J Pilkis
Isolation and characterization of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase from bovine liver.
Archives of biochemistry and biophysics 1985;238(2):531-43.
1984: M Raafat El-Maghrabi; T M Pate; J Pilkis; S J Pilkis
Effect of sulfhydryl modification on the activities of rat liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase.
The Journal of biological chemistry 1984;259(21):13104-10.
1984: M Raafat El-Maghrabi; T M Pate; K J Murray; S J Pilkis
Differential effects of proteolysis and protein modification on the activities of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase.
The Journal of biological chemistry 1984;259(21):13096-103.
1984: M Raafat El-Maghrabi; T M Pate; S J Pilkis
Characterization of the exchange reactions of rat liver 6-phosphofructo 2-kinase/fructose 2,6-bisphosphatase.
Biochemical and biophysical research communications 1984;123(2):749-56.
1984: K J Murray; M Raafat El-Maghrabi; Paul Kountz; T J Lukas; Thomas R Soderling; S J Pilkis
Amino acid sequence of the phosphorylation site of rat liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase.
The Journal of biological chemistry 1984;259(12):7673-81.
1984: S J Pilkis; D M Regen; H B Stewart; J Pilkis; T M Pate; M Raafat El-Maghrabi
Evidence for two catalytic sites on 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase. Dynamics of substrate exchange and phosphoryl enzyme formation.
The Journal of biological chemistry 1984;259(2):949-58.
1984: T H Claus; M Raafat El-Maghrabi; D M Regen; H B Stewart; M McGrane; Paul Kountz; F Nyfeler; J Pilkis; S J Pilkis
The role of fructose 2,6-bisphosphate in the regulation of carbohydrate metabolism.
Current topics in cellular regulation 1984;23():57-86.
1983: M M McGrane; M Raafat El-Maghrabi; S J Pilkis
Evidence for different forms of rat liver fructose 1,6-bisphosphatase.
Biochemical and biophysical research communications 1983;117(3):751-7.
1983: M M McGrane; M Raafat El-Maghrabi; S J Pilkis
The interaction of fructose 2,6-bisphosphate and AMP with rat hepatic fructose 1,6-bisphosphatase.
The Journal of biological chemistry 1983;258(17):10445-54.
1983: S J Pilkis; Mark Walderhaug; K Murray; Albert H Beth; S D Venkataramu; J Pilkis; M Raafat El-Maghrabi
6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase from rat liver.
The Journal of biological chemistry 1983;258(10):6135-41.
1983: S J Pilkis; T D Chrisman; M Raafat El-Maghrabi; A Colosia; E Fox; J Pilkis; T H Claus
The action of insulin on hepatic fructose 2,6-bisphosphate metabolism.
The Journal of biological chemistry 1983;258(3):1495-503.
1983: M Raafat El-Maghrabi; T H Claus; S J Pilkis
Substrate-directed regulation of cAMP-dependent phosphorylation.
Methods in enzymology 1983;99():212-9.
1983: S J Pilkis; T Chrisman; B Burgress; M McGrane; A Colosia; J Pilkis; T H Claus; M Raafat El-Maghrabi
Rat hepatic 6-phosphofructo 2-kinase/fructose 2,6-bisphosphatase: a unique bifunctional enzyme.
Advances in enzyme regulation 1983;21():147-73.
1982: S J Pilkis; M Raafat El-Maghrabi; M McGrane; J Pilkis; T H Claus
Regulation by glucagon of hepatic pyruvate kinase, 6-phosphofructo 1-kinase, and fructose-1,6-bisphosphatase.
Federation proceedings 1982;41(10):2623-8.
1982: M Raafat El-Maghrabi; T H Claus; J Pilkis; E Fox; S J Pilkis
Regulation of rat liver fructose 2,6-bisphosphatase.
The Journal of biological chemistry 1982;257(13):7603-7.
1982: T H Claus; J R Schlumpf; M Raafat El-Maghrabi; S J Pilkis
Regulation of the phosphorylation and activity of 6-phosphofructo 1-kinase in isolated hepatocytes by alpha-glycerolphosphate and fructose 2,6-bisphosphate.
The Journal of biological chemistry 1982;257(13):7541-8.
1982: M Raafat El-Maghrabi; E Fox; J Pilkis; S J Pilkis
Cyclic AMP-dependent phosphorylation of rat liver 6-phosphofructo 2-kinase, fructose 2,6-bisphosphatase.
Biochemical and biophysical research communications 1982;106(3):794-802.
1982: S J Pilkis; M Raafat El-Maghrabi; J Pilkis; T H Claus
Studies on the in vitro phosphorylation of 6-phosphofructo-1-kinase from rat liver.
Archives of biochemistry and biophysics 1982;215(2):379-89.
1982: S J Pilkis; M Raafat El-Maghrabi; M McGrane; J Pilkis; E Fox; T H Claus
Fructose 2,6-bisphosphate: a mediator of hormone action at the fructose 6-phosphate/fructose 1,6-bisphosphate substrate cycle.
Molecular and cellular endocrinology 1982;25(3):245-66.
1982: M Raafat El-Maghrabi; T H Claus; M M McGrane; S J Pilkis
Influence of phosphorylation on the interaction of effectors with rat liver pyruvate kinase.
The Journal of biological chemistry 1982;257(1):233-40.
1982: M Raafat El-Maghrabi; T H Claus; J Pilkis; S J Pilkis
Regulation of 6-phosphofructo-2-kinase activity by cyclic AMP-dependent phosphorylation.
Proceedings of the National Academy of Sciences of the United States of America 1982;79(2):315-9.
1981: P Neely; M Raafat El-Maghrabi; S J Pilkis; T H Claus
Effect of diabetes, insulin, starvation, and refeeding on the level of rat hepatic fructose 2,6-bisphosphate.
Diabetes 1981;30(12):1062-4.
1981: S J Pilkis; M Raafat El-Maghrabi; M M McGrane; J Pilkis; T H Claus
The role of fructose 2,6-bisphosphate in regulation of fructose-1,6-bisphosphatase.
The Journal of biological chemistry 1981;256(22):11489-95.
1981: M Raafat El-Maghrabi; T H Claus; J Pilkis; S J Pilkis
Partial purification of a rat liver enzyme that catalyzes the formation of fructose 2,6-bisphosphate.
Biochemical and biophysical research communications 1981;101(3):1071-7.
1981: S J Pilkis; M Raafat El-Maghrabi; J Pilkis; T Claus
Inhibition of fructose-1,6-bisphosphatase by fructose 2,6-bisphosphate.
The Journal of biological chemistry 1981;256(8):3619-22.
1981: S J Pilkis; M Raafat El-Maghrabi; J Pilkis; T H Claus; D A Cumming
Fructose 2,6-bisphosphate. A new activator of phosphofructokinase.
The Journal of biological chemistry 1981;256(7):3171-4.
1980: T H Claus; J R Schlumpf; M Raafat El-Maghrabi; J Pilkis; S J Pilkis
Mechanism of action of glucagon on hepatocyte phosphofructokinase activity.
Proceedings of the National Academy of Sciences of the United States of America 1980;77(11):6501-5.
1980: S J Pilkis; M Raafat El-Maghrabi; B Coven; T H Claus; H S Tager; D F Steiner; P S Keim; R L Heinrikson
Phosphorylation of rat hepatic fructose-1,6-bisphosphatase and pyruvate kinase.
The Journal of biological chemistry 1980;255(7):2770-5.
1980: M Raafat El-Maghrabi; W S Haston; D A Flockhart; T H Claus; S J Pilkis
Studies on the phosphorylation and dephosphorylation of L-type pyruvate kinase by the catalytic subunit of cyclic AMP-dependent protein kinase.
The Journal of biological chemistry 1980;255(2):668-75.