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José Luis Fernández

Research Profile

Procedures

Anatomy

Physiology

DNA Fragmentation

Disorders

DNA Damage

Chemicals & Drugs

Activities & Behaviors

Image Processing, Computer-Assisted

Living Beings

Cricetinae

Co-author Network

Publications

TOP 3
Rebeca Santiso; María Tamayo; Carlos Fernández; Jaime Gosálvez; Steve Johnston; Carlos Losada; Alfonso Mariño; José Luis Fernández
DNA fragmentation dynamics allows the assessment of cryptic sperm damage in human: evaluation of exposure to ionizing radiation, hyperthermia, acidic pH and nitric oxide.
C González-Marín; R Roy; Carmen López-Fernández; B Diez; M J Carabaño; José Luis Fernández; M E Kjelland; J F Moreno; Jaime Gosálvez
Bacteria in bovine semen can increase sperm DNA fragmentation rates: a kinetic experimental approach.
Carmen López-Fernández; Stephen D Johnston; José Luis Fernández; R J Wilson; Jaime Gosálvez
Fragmentation dynamics of frozen-thawed ram sperm DNA is modulated by sperm concentration.

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All Publications

2012: Rebeca Santiso; María Tamayo; Carlos Fernández; Jaime Gosálvez; Steve Johnston; Carlos Losada; Alfonso Mariño; José Luis Fernández
DNA fragmentation dynamics allows the assessment of cryptic sperm damage in human: evaluation of exposure to ionizing radiation, hyperthermia, acidic pH and nitric oxide.
Mutation research 2012;734(1-2):41-9.
2011: C González-Marín; R Roy; Carmen López-Fernández; B Diez; M J Carabaño; José Luis Fernández; M E Kjelland; J F Moreno; Jaime Gosálvez
Bacteria in bovine semen can increase sperm DNA fragmentation rates: a kinetic experimental approach.
Animal reproduction science 2011;123(3-4):139-48.
2010: Carmen López-Fernández; Stephen D Johnston; José Luis Fernández; R J Wilson; Jaime Gosálvez
Fragmentation dynamics of frozen-thawed ram sperm DNA is modulated by sperm concentration.
Theriogenology 2010;74(8):1362-70.
2009: Jaime Gosálvez; Elva I Cortés-Gutiérrez; Rocio Nuñez; José Luis Fernández; Pedro Caballero; Carmen López-Fernández; William V Holt
A dynamic assessment of sperm DNA fragmentation versus sperm viability in proven fertile human donors.
Fertility and sterility 2009;92(6):1915-9.
2009: Carmen López-Fernández; Matthew J G Gage; Francisca Arroyo; Altea Gosálbez; Ana M Larrán; José Luis Fernández; Jaime Gosálvez
Rapid rates of sperm DNA damage after activation in tench (Tinca tinca: Teleostei, Cyprinidae) measured using a sperm chromatin dispersion test.
Reproduction (Cambridge, England) 2009;138(2):257-66.
2009: Jaime Gosálvez; Elva Cortés-Gutierez; Carmen López-Fernández; José Luis Fernández; Pedro Caballero; Rocio Nuñez
Sperm deoxyribonucleic acid fragmentation dynamics in fertile donors.
Fertility and sterility 2009;92(1):170-3.
2008: Carmen López-Fernández; José Luis Fernández; Altea Gosálbez; Francisca Arroyo; J M Vázquez; William V Holt; Jaime Gosálvez
Dynamics of sperm DNA fragmentation in domestic animals III. Ram.
Theriogenology 2008;70(6):898-908.
2008: Adriana Cerná; Carmen López-Fernández; José Luis Fernández; Susana Moreno Díaz de la Espina; Consuelo De la Torre; Jaime Gosálvez
Triplex configuration in the nick-free DNAs that constitute the chromosomal scaffolds in grasshopper spermatids.
Chromosoma 2008;117(1):15-24.
2008: Carmen López-Fernández; Begoña Pérez-Llano; Pedro García-Casado; R Sala; Altea Gosálbez; Francisca Arroyo; José Luis Fernández; Jaime Gosálvez
Sperm DNA fragmentation in a random sample of the Spanish boar livestock.
Animal reproduction science 2008;103(1-2):87-98.
2007: Carmen López-Fernández; F Crespo; Francisca Arroyo; José Luis Fernández; P Arana; Stephen D Johnston; Jaime Gosálvez
Dynamics of sperm DNA fragmentation in domestic animals II. The stallion.
Theriogenology 2007;68(9):1240-50.
2007: Thamara Viloria; Nicolás Garrido; José Luis Fernández; José Remohí; Antonio Pellicer; Marcos Meseguer
Sperm selection by swim-up in terms of deoxyribonucleic acid fragmentation as measured by the sperm chromatin dispersion test is altered in heavy smokers.
Fertility and sterility 2007;88(2):523-5.
2007: Joaquina de la Torre; Carmen López-Fernández; Miguel Pita; José Luis Fernández; Stephen D Johnston; Jaime Gosálvez
Simultaneous observation of DNA fragmentation and protein loss in the boar spermatozoon following application of the sperm chromatin dispersion (SCD) test.
Journal of andrology 2007;28(4):533-40.
2007: Miguel Pita; José Luis Fernández; Jaime Gosálvez
Whole-comparative genomic hybridization and "cell code" estimation: an application for assessment of cellular chimerism.
European journal of medical research 2007;12(5):206-11.
2007: Rebeca Santiso; Lourdes Muriel; Vicente Goyanes; Enrique Segrelles; Jaime Gosálvez; José Luis Fernández
Evidence of modified nuclear protein matrix in human spermatozoa with fragmented deoxyribonucleic acid.
Fertility and sterility 2007;87(1):191-4.
2007: Lourdes Muriel; Vicente Goyanes; Enrique Segrelles; Jaime Gosálvez; Juan G Alvarez; José Luis Fernández
Increased aneuploidy rate in sperm with fragmented DNA as determined by the sperm chromatin dispersion (SCD) test and FISH analysis.
Journal of andrology 2007;28(1):38-49.
2006: Carmen López-Fernández; Francisca Arroyo; José Luis Fernández; Jaime Gosálvez
Interstitial telomeric sequence blocks in constitutive pericentromeric heterochromatin from Pyrgomorpha conica (Orthoptera) are enriched in constitutive alkali-labile sites.
Mutation research 2006;599(1-2):36-44.
2006: Lourdes Muriel; Marcos Meseguer; José Luis Fernández; Juan Alvarez; José Remohí; Antonio Pellicer; Nicolás Garrido
Value of the sperm chromatin dispersion test in predicting pregnancy outcome in intrauterine insemination: a blind prospective study.
Human reproduction (Oxford, England) 2006;21(3):738-44.
2006: Lourdes Muriel; Nicolás Garrido; José Luis Fernández; José Remohí; Antonio Pellicer; María-José de los Santos; Marcos Meseguer
Value of the sperm deoxyribonucleic acid fragmentation level, as measured by the sperm chromatin dispersion test, in the outcome of in vitro fertilization and intracytoplasmic sperm injection.
Fertility and sterility 2006;85(2):371-83.
2006: Maria Enciso; Carmen López-Fernández; José Luis Fernández; Pedro García; Altea Gosálbez; Jaime Gosálvez
A new method to analyze boar sperm DNA fragmentation under bright-field or fluorescence microscopy.
Theriogenology 2006;65(2):308-16.
2006: Maria Enciso; Lourdes Muriel; José Luis Fernández; Vicente Goyanes; Enrique Segrelles; Mercedes Marcos; Juan Manuel Montejo; Manolo Ardoy; Alberto Pacheco; Jaime Gosálvez
Infertile men with varicocele show a high relative proportion of sperm cells with intense nuclear damage level, evidenced by the sperm chromatin dispersion test.
Journal of andrology 2006;27(1):106-11.
2005: José Luis Fernández; Lourdes Muriel; Vicente Goyanes; Enrique Segrelles; Jaime Gosálvez; Maria Enciso; Marie LaFromboise; Christopher De Jonge
Halosperm is an easy, available, and cost-effective alternative for determining sperm DNA fragmentation.
Fertility and sterility 2005;84(4):860.
2005: Silvia Rodríguez; Vicente Goyanes; Enrique Segrelles; María Blasco; Jaime Gosálvez; José Luis Fernández
Critically short telomeres are associated with sperm DNA fragmentation.
Fertility and sterility 2005;84(4):843-5.
2005: José Luis Fernández; Lourdes Muriel; Vicente Goyanes; Enrique Segrelles; Jaime Gosálvez; Maria Enciso; Marie LaFromboise; Christopher De Jonge
Simple determination of human sperm DNA fragmentation with an improved sperm chromatin dispersion test.
Fertility and sterility 2005;84(4):833-42.
2005: Alejandro Mosquera; Jaime Gosálvez; Laure Sabatier; José Luis Fernández
Interstitial telomeric DNA sequences of Chinese hamster cells are hypersensitive to nitric oxide damage, and DNA-PKcs has a specific local role in its repair.
Genes, chromosomes & cancer 2005;44(1):76-84.
2005: Raquel Losada; María Teresa Rivero; Predrag Slijepcevic; Vicente Goyanes; José Luis Fernández
Effect of Wortmannin on the repair profiles of DNA double-strand breaks in the whole genome and in interstitial telomeric sequences of Chinese hamster cells.
Mutation research 2005;570(1):119-28.
2004: Carmen López-Fernández; E Pradillo; M Zabal-Aguirre; José Luis Fernández; C García de la Vega; Jaime Gosálvez
Telomeric and interstitial telomeric-like DNA sequences in Orthoptera genomes.
Genome / National Research Council Canada = Génome / Conseil national de recherches Canada 2004;47(4):757-63.
2004: María Teresa Rivero; Alejandro Mosquera; Vicente Goyanes; Predrag Slijepcevic; José Luis Fernández
Differences in repair profiles of interstitial telomeric sites between normal and DNA double-strand break repair deficient Chinese hamster cells.
Experimental cell research 2004;295(1):161-72.
2004: Lourdes Muriel; Enrique Segrelles; Vicente Goyanes; Jaime Gosálvez; José Luis Fernández
Structure of human sperm DNA and background damage, analysed by in situ enzymatic treatment and digital image analysis.
Molecular human reproduction 2004;10(3):203-9.
2003: María Teresa Rivero; Fernando Vázquez-Gundín; Lourdes Muriel; Vicente Goyanes; Jaime Gosálvez; José Luis Fernández
Patterns of DNA migration in two-dimensional single-cell gel electrophoresis analyzed by DNA breakage detection-fluorescence in situ hybridization.
Environmental and molecular mutagenesis 2003;42(3):223-7.
2003: José Luis Fernández; Lourdes Muriel; María Teresa Rivero; Vicente Goyanes; Rosana Vazquez; Juan G Alvarez
The sperm chromatin dispersion test: a simple method for the determination of sperm DNA fragmentation.
Journal of andrology 2003;24(1):59-66.
2002: José Luis Fernández; Jaime Gosálvez
Application of FISH to detect DNA damage. DNA breakage detection-FISH (DBD-FISH).
Methods in molecular biology (Clifton, N.J.) 2002;203():203-16.
2001: María Teresa Rivero; Fernando Vázquez-Gundín; Vicente Goyanes; A Campos; M Blasco; Jaime Gosálvez; José Luis Fernández
High frequency of constitutive alkali-labile sites in mouse major satellite DNA, detected by DNA breakage detection-fluorescence in situ hybridization.
Mutation research 2001;483(1-2):43-50.
2001: José Luis Fernández; Fernando Vázquez-Gundín; María Teresa Rivero; A Genescá; Jaime Gosálvez; Vicente Goyanes
DBD-fish on neutral comets: simultaneous analysis of DNA single- and double-strand breaks in individual cells.
Experimental cell research 2001;270(1):102-9.
2001: José Luis Fernández; Fernando Vázquez-Gundín; María Teresa Rivero; Vicente Goyanes; Jaime Gosálvez
Evidence of abundant constitutive alkali-labile sites in human 5 bp classical satellite DNA loci by DBD-FISH.
Mutation research 2001;473(2):163-8.
2001: Ismael Buño; José Luis Fernández; Carmen López-Fernández; José L Díez-Martín; Jaime Gosálvez
Sau3A in situ digestion of human chromosome 3 pericentrometric heterochromatin. I. Differential digestion of alpha-satellite and satellite 1 DNA sequences.
Genome / National Research Council Canada = Génome / Conseil national de recherches Canada 2001;44(1):120-7.
2000: Fernando Vázquez-Gundín; Jaime Gosálvez; J de la Torre; José Luis Fernández
DNA breakage detection-fish (DBD-FISH): effect of unwinding time.
Mutation research 2000;453(1):83-8.
2000: José Luis Fernández; Fernando Vázquez-Gundín; A Delgado; Vicente Goyanes; JL Ramiro-Díaz; J de la Torre; Jaime Gosálvez
DNA breakage detection-FISH (DBD-FISH) in human spermatozoa: technical variants evidence different structural features.
Mutation research 2000;453(1):77-82.
2000: Joaquina de la Torre; Angel Martínez-Ramírez; José Luis Fernández; José L Díez-Martín; Alfonso Gómez-Pineda; Jaime Gosálvez; Carmen López-Fernández
A PCR product derived from female DNA with regional localization on the Y chromosome.
Genome / National Research Council Canada = Génome / Conseil national de recherches Canada 2000;43(3):580-3.
1999: Ismael Buño; Joaquina de la Torre; E Torroja; José Luis Fernández; Carmen López-Fernández; José L Díez-Martín; Jaime Gosálvez
Chimerism quantification after sex-matched BMT: how probable is it to find donor/recipient pairs with distinguishable cells?
Cancer genetics and cytogenetics 1999;113(2):152-5.
1999: Ismael Buño; José L Díez-Martín; Carmen López-Fernández; José Luis Fernández; Jaime Gosálvez
Polymorphisms for the size of heterochromatic regions allow sex-independent quantification of post-BMT chimerism targeting metaphase and interphase cells.
Haematologica 1999;84(2):138-41.
1998: Diana Valverde; Fernando Vázquez-Gundín; Elisabeth del Rio; M Calaf; José Luis Fernández; Montserrat Baiget
Analysis of the IRBP gene as a cause of RP in 45 ARRP Spanish families. Autosomal recessive retinitis pigmentosa. Interstitial retinol binding protein. Spanish Multicentric and Multidisciplinary Group for Research into Retinitis Pigmentosa.
Ophthalmic genetics 1998;19(4):197-202.
1998: José Luis Fernández; Vicente Goyanes; JL Ramiro-Díaz; Jaime Gosálvez
Application of FISH for in situ detection and quantification of DNA breakage.
Cytogenetics and cell genetics 1998;82(3-4):251-6.
1998: José Luis Fernández; Vicente Goyanes; JL Ramiro-Díaz; Jaime Gosálvez
Evidence of a differential organization of chromatin containing terminal or interstitial (TTAGGG)n repeats by in situ digestion with nucleases.
Cytogenetics and cell genetics 1998;82(3-4):195-8.
1997: Jaime Gosálvez; Ismael Buño; Carmen López-Fernández; José Luis Fernández; Vicente Goyanes
Image analysis of murine chromatin fiber structure after in situ digestion with restriction endonucleases.
Analytical and quantitative cytology and histology / the International Academy of Cytology [and] American Society of Cytology 1997;19(3):207-14.
1997: José Luis Fernández; Diana Valverde; Vicente Goyanes; Ismael Buño; Jaime Gosálvez
AluI in situ digestion of human alphoid and classical satellite DNA regions: high-resolution digital image analysis of FISH signals from condensed and extended chromatin.
Cytogenetics and cell genetics 1997;76(1-2):94-100.
1996: José Luis Fernández; Carmen López-Fernández; Jaime Gosálvez; Vicente Goyanes
Differential sensitivity of some human alphoid and classical satellite DNA regions from lymphocyte chromosomes to in situ exonuclease III digestion.
Genome / National Research Council Canada = Génome / Conseil national de recherches Canada 1996;39(6):1210-3.
1996: Jaime Gosálvez; Carmen López-Fernández; Ismael Buño; N Polo; Pilar Llamas; Manuel-Nicolás Fernández; José Luis Fernández; José L Díez-Martín
Restriction endonuclease in situ digestion (REISD) and fluorescence in situ hybridization (FISH) as complementary methods to analyze chimerism and residual disease after bone marrow transplantation.
Cancer genetics and cytogenetics 1996;89(2):141-5.
1996: Ismael Buño; Carmen López-Fernández; José Luis Fernández; Pilar Llamas; J L Díez-Martin; Jaime Gosálvez
Improving chimaerism quantification in bone marrow transplant recipients by image processing and analysis after restriction endonuclease in situ digestion (IPA-REISD).
Leukemia 1996;10(7):1232-6.
1996: Jaime Gosálvez; Carmen López-Fernández; José Luis Fernández; Vicente Goyanes; Ismael Buño
Digital image analysis of chromatin fibre phenotype after "in situ' digestion with restriction endonucleases.
Cell biology international 1996;20(3):213-7.
1996: José Luis Fernández; Diana Valverde; Jaime Gosálvez; C Pineiro; S Pereira; Vicente Goyanes
Chromosome fragments with alphoid sequences derived from a pseudoisodicentric Y chromosome.
Journal of medical genetics 1996;33(1):84-6.
1996: José Luis Fernández; Vicente Goyanes; Carmen López-Fernández; Ismael Buño; Jaime Gosálvez
Quantification of C-ERB-B2 gene amplification in breast cancer cells using fluorescence in situ hybridization and digital image analysis.
Cancer genetics and cytogenetics 1996;86(1):18-21.
1995: J L Bella; José Luis Fernández; Jaime Gosálvez
C-banding plus fluorochrome staining shows differences in C-, G-, and R-bands in human and mouse metaphase chromosomes.
Genome / National Research Council Canada = Génome / Conseil national de recherches Canada 1995;38(5):864-8.
1995: Jaime Gosálvez; Carmen López-Fernández; José Luis Fernández; Vicente Goyanes; Ismael Buño
Digital image analysis of chromatin fibre phenotype after "in situ" digestion with restriction endonucleases.
Cell biology international 1995;19(10):827-32.
1995: José Luis Fernández; Jaime Gosálvez; Vicente Goyanes
High frequency of mutagen-induced chromatid exchanges at interstitial telomere-like DNA sequence blocks of Chinese hamster cells.
Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology 1995;3(5):281-4.
1995: José Luis Fernández; A Campos; V Goyanes; C Losada; C Veiras; A A Edwards
X-ray biological dosimetry performed by selective painting of human chromosomes 1 and 2.
International journal of radiation biology 1995;67(3):295-302.
1995: José Luis Fernández; A Campos; Carmen López-Fernández; Jaime Gosálvez; Vicente Goyanes
Difference in constitutive heterochromatin behaviour between human amniocytes and lymphocytes detected by a sequential in situ exonuclease III digestion-random primer extension procedure.
Journal of medical genetics 1995;32(1):32-5.
1994: José Luis Fernández; S Pereira; A Campos; Vicente Goyanes
Assessment of Yqh translocations.
Journal of medical genetics 1994;31(12):978-9.
1994: José Luis Fernández; S Pereira; A Campos; Jaime Gosálvez; Vicente Goyanes
An extra band within the human 9qh+ region that behaves like the surrounding constitutive heterochromatin.
Journal of medical genetics 1994;31(8):632-4.
1994: V Goyanes; José Luis Fernández; S Pereira; A Campos; E Rodriguez; M González; M Rodriguez; O Espido; M García
Frequency of sister chromatid exchanges in humans at 14-16 week foetal stage and at birth.
Cytobios 1994;77(309):67-72.
1994: José Luis Fernández; A Campos; Vicente Goyanes; Ismael Buño; Jaime Gosálvez
Sister chromatid differentiation after in situ detection of ultraviolet-induced DNA breaks under electron microscopy.
Biology of the cell / under the auspices of the European Cell Biology Organization 1994;82(1):33-7.
1994: José Luis Fernández; Vicente Goyanes; Carmen López-Fernández; Jaime Gosálvez
Human alpha-satellite DNAs are not susceptible to undercondensation after 5-azacytidine treatment.
Cytogenetics and cell genetics 1994;65(1-2):92-4.
1994: José Luis Fernández; Vicente Goyanes; S Pereira; Carmen López-Fernández; Jaime Gosálvez
5-azacytidine produces differential undercondensation of alpha, beta and classical human satellite DNAs.
Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology 1994;2(1):29-35.
1993: Jaime Gosálvez; Carmen López-Fernández; José Luis Fernández; Vicente Goyanes
Oligonucleotide priming in situ to detect induced DNA breaks under electron microscopy.
Trends in genetics : TIG 1993;9(5):156-7.
1993: Jaime Gosálvez; Carmen López-Fernández; C García de la Vega; R Mezzanotte; José Luis Fernández; Vicente Goyanes
Selective digestion of mouse chromosomes with restriction endonucleases. Oligonucleotide priming of single-stranded DNA produced with exonuclease III.
Genome / National Research Council Canada = Génome / Conseil national de recherches Canada 1993;36(2):230-4.
1993: José Luis Fernández; Jaime Gosálvez; Vicente Goyanes
Detection of DNA strand breaks induced by hydroxyl radicals in nuclear and chromosomal chromatin by electron microscopy.
Cytobios 1993;73(294-295):189-95.