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Luís Ferreira

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Ana S Luís; Shabir Najmudin; José A M Prates; Artur Rogowski; Max J Temple; Immacolata Venditto; Jie Xue; Arnaud Baslé; Luís M A Ferreira; Carlos M G A Fontes; J Paul Knox; Harry J Gilbert
Understanding how noncatalytic carbohydrate binding modules can display specificity for xyloglucan.
Immacolata Venditto; Max J Temple; Arnaud Baslé; Luís M A Ferreira; Carlos M G A Fontes; Harry J Gilbert; Ana S Luís; Shabir Najmudin
Overproduction, purification, crystallization and preliminary X-ray characterization of the C-terminal family 65 carbohydrate-binding module (CBM65B) of endoglucanase Cel5A from Eubacterium cellulosolvens.
Joana L A Brás; Ana L Carvalho; Luís M A Ferreira; Harry J Gilbert; Shabir Najmudin; José A M Prates; Maria João Romão; Victor D Alves; David N Bolam; Carlos M G A Fontes
Novel Clostridium thermocellum type I cohesin-dockerin complexes reveal a single binding mode.

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All Publications

2013: Ana S Luís; Shabir Najmudin; José A M Prates; Artur Rogowski; Max J Temple; Immacolata Venditto; Jie Xue; Arnaud Baslé; Luís M A Ferreira; Carlos M G A Fontes; J Paul Knox; Harry J Gilbert
Understanding how noncatalytic carbohydrate binding modules can display specificity for xyloglucan.
The Journal of biological chemistry 2013;288(7):4799-809.
2013: Immacolata Venditto; Max J Temple; Arnaud Baslé; Luís M A Ferreira; Carlos M G A Fontes; Harry J Gilbert; Ana S Luís; Shabir Najmudin
Overproduction, purification, crystallization and preliminary X-ray characterization of the C-terminal family 65 carbohydrate-binding module (CBM65B) of endoglucanase Cel5A from Eubacterium cellulosolvens.
Acta crystallographica. Section F, Structural biology and crystallization communications 2013;69(Pt 2):191-4.
2012: Joana L A Brás; Ana L Carvalho; Luís M A Ferreira; Harry J Gilbert; Shabir Najmudin; José A M Prates; Maria João Romão; Victor D Alves; David N Bolam; Carlos M G A Fontes
Novel Clostridium thermocellum type I cohesin-dockerin complexes reveal a single binding mode.
The Journal of biological chemistry 2012;287(53):44394-405.
2012: Kate Cameron; Luís M A Ferreira; Carlos M G A Fontes; Victor D Alves; Pedro Bule; Shabir Najmudin
Purification, crystallization and preliminary X-ray characterization of the Acetivibrio cellulolyticus type I cohesin ScaC in complex with the ScaB dockerin.
Acta crystallographica. Section F, Structural biology and crystallization communications 2012;68(Pt 9):1030-3.
2012: Joana L A Brás; Ana Luisa Carvalho; Luís M A Ferreira; Harry J Gilbert; Shabir Najmudin; José A M Prates; Maria João Romão; Aldino Viegas; Victor D Alves; Carlos M G A Fontes
Escherichia coli expression, purification, crystallization, and structure determination of bacterial cohesin-dockerin complexes.
Methods in enzymology 2012;510():395-415.
2010: Teresa Ribeiro; Teresa Santos-Silva; Victor D Alves; Fernando M V Dias; Ana S Luís; José A M Prates; Luís M A Ferreira; Maria J Romão; Carlos M G A Fontes
Family 42 carbohydrate-binding modules display multiple arabinoxylan-binding interfaces presenting different ligand affinities.
Biochimica et biophysica acta 2010;1804(10):2054-62.
2009: Márcia A S Correia; Virgínia M R Pires; Harry J Gilbert; David N Bolam; Vânia O Fernandes; Victor D Alves; José A M Prates; Luís M A Ferreira; Carlos M G A Fontes
Family 6 carbohydrate-binding modules display multiple beta1,3-linked glucan-specific binding interfaces.
FEMS microbiology letters 2009;300(1):48-57.
2008: P I P Ponte; J A M Prates; J P Crespo; D G Crespo; J L Mourão; S P Alves; R J B Bessa; M A Chaveiro-Soares; L T Gama; Luís M A Ferreira; C M G A Fontes
Restricting the intake of a cereal-based feed in free-range-pastured poultry: effects on performance and meat quality.
Poultry science 2008;87(10):2032-42.
2008: T Ribeiro; P I P Ponte; Catarina I P D Guerreiro; H M Santos; L Falcão; J P B Freire; Luís M A Ferreira; José A M Prates; Carlos M G A Fontes; M M Lordelo
A family 11 carbohydrate-binding module (CBM) improves the efficacy of a recombinant cellulase used to supplement barley-based diets for broilers at lower dosage rates.
British poultry science 2008;49(5):600-8.
2008: P I P Ponte; J A M Prates; J P Crespo; D G Crespo; J L Mourão; S P Alves; R J B Bessa; M A Chaveiro-Soares; Luís M A Ferreira; C M G A Fontes
Improving the lipid nutritive value of poultry meat through the incorporation of a dehydrated leguminous-based forage in the diet for broiler chicks.
Poultry science 2008;87(8):1587-94.
2008: Catarina I P D Guerreiro; T Ribeiro; P I P Ponte; M M S Lordelo; L Falcao; J P B Freire; Luís M A Ferreira; José A M Prates; Carlos M G A Fontes
Role of a family 11 carbohydrate-binding module in the function of a recombinant cellulase used to supplement a barley-based diet for broiler chickens.
British poultry science 2008;49(4):446-54.
2008: P I P Ponte; M M S Lordelo; Catarina I P D Guerreiro; M C Soares; J L Mourao; J P Crespo; D G Crespo; José A M Prates; Luís M A Ferreira; Carlos M G A Fontes
Crop beta-glucanase activity limits the effectiveness of a recombinant cellulase used to supplement a barley-based feed for free-range broilers.
British poultry science 2008;49(3):347-59.
2008: P I P Ponte; SP Alves; R J B Bessa; Luís M A Ferreira; LT Gama; J L A Brás; Carlos M G A Fontes; José A M Prates
Influence of pasture intake on the fatty acid composition, and cholesterol, tocopherols, and tocotrienols content in meat from free-range broilers.
Poultry science 2008;87(1):80-8.
2008: P I P Ponte; C M C Rosado; J P Crespo; D G Crespo; J L Mourão; M A Chaveiro-Soares; J L A Brás; I Mendes; LT Gama; José A M Prates; Luís M A Ferreira; Carlos M G A Fontes
Pasture intake improves the performance and meat sensory attributes of free-range broilers.
Poultry science 2008;87(1):71-9.
2007: Ana L Carvalho; Fernando M V Dias; Tibor Nagy; José A M Prates; Mark R Proctor; Nicola Smith; Edward A Bayer; Gideon J Davies; Luís M A Ferreira; Maria João Romão; Carlos M G A Fontes; Harry J Gilbert
Evidence for a dual binding mode of dockerin modules to cohesins.
Proceedings of the National Academy of Sciences of the United States of America 2007;104(9):3089-94.
2006: Maria S J Centeno; Arun Goyal; José A M Prates; Luís M A Ferreira; Harry J Gilbert; Carlos M G A Fontes
Novel modular enzymes encoded by a cellulase gene cluster in Cellvibrio mixtus.
FEMS microbiology letters 2006;265(1):26-34.
2006: Carlos Martinez-Fleites; Catarina I P D Guerreiro; Martin J Baumann; Edward J Taylor; José A M Prates; Luís M A Ferreira; Carlos M G A Fontes; Harry Brumer; Gideon J Davies
Crystal structures of Clostridium thermocellum xyloglucanase, XGH74A, reveal the structural basis for xyloglucan recognition and degradation.
The Journal of biological chemistry 2006;281(34):24922-33.
2006: Maria S J Centeno; Catarina I P D Guerreiro; Fernando M V Dias; Carl Morland; Louise E Tailford; Arun Goyal; José A M Prates; Luís M A Ferreira; Rui M H Caldeira; Emmanuel F Mongodin; Karen E Nelson; Harry J Gilbert; Carlos M G A Fontes
Galactomannan hydrolysis and mannose metabolism in Cellvibrio mixtus.
FEMS microbiology letters 2006;261(1):123-32.
2006: Edward J Taylor; Tracey M Gloster; Johan P Turkenburg; Florence Vincent; Andrzej M Brzozowski; Claude Dupont; François Shareck; Maria S J Centeno; José A M Prates; Vladimír Puchart; Luís M A Ferreira; Carlos M G A Fontes; Peter Biely; Gideon J Davies
Structure and activity of two metal ion-dependent acetylxylan esterases involved in plant cell wall degradation reveals a close similarity to peptidoglycan deacetylases.
The Journal of biological chemistry 2006;281(16):10968-75.
2006: Shabir Najmudin; Catarina I P D Guerreiro; Ana L Carvalho; José A M Prates; Márcia A S Correia; Victor D Alves; Luís M A Ferreira; Maria João Romão; Harry J Gilbert; David N Bolam; Carlos M G A Fontes
Xyloglucan is recognized by carbohydrate-binding modules that interact with beta-glucan chains.
The Journal of biological chemistry 2006;281(13):8815-28.
2005: Shabir Najmudin; Catarina I P D Guerreiro; Luís M A Ferreira; Maria João Romão; Carlos M G A Fontes; José A M Prates
Overexpression, purification and crystallization of the two C-terminal domains of the bifunctional cellulase ctCel9D-Cel44A from Clostridium thermocellum.
Acta crystallographica. Section F, Structural biology and crystallization communications 2005;61(Pt 12):1043-5.
2005: Ana L Carvalho; Virginia M R Pires; Tracey M Gloster; Johan P Turkenburg; José A M Prates; Luís M A Ferreira; Maria João Romão; Gideon J Davies; Carlos M G A Fontes; Harry J Gilbert
Insights into the structural determinants of cohesin-dockerin specificity revealed by the crystal structure of the type II cohesin from Clostridium thermocellum SdbA.
Journal of molecular biology 2005;349(5):909-15.
2004: Carlos M G A Fontes; P I P Ponte; T C Reis; MC Soares; LT Gama; Fernando M V Dias; Luís M A Ferreira
A family 6 carbohydrate-binding module potentiates the efficiency of a recombinant xylanase used to supplement cereal-based diets for poultry.
British poultry science 2004;45(5):648-56.
2004: Fernando M V Dias; Arun Goyal; Harry J Gilbert; Luís M A Ferreira; Carlos M G A Fontes
The N-terminal family 22 carbohydrate-binding module of xylanase 10B of Clostridium themocellum is not a thermostabilizing domain.
FEMS microbiology letters 2004;238(1):71-8.
2004: Ana L Carvalho; Arun Goyal; José A M Prates; David N Bolam; Harry J Gilbert; Virgínia M R Pires; Luís M A Ferreira; Antoni Planas; Maria João Romão; Carlos M G A Fontes
The family 11 carbohydrate-binding module of Clostridium thermocellum Lic26A-Cel5E accommodates beta-1,4- and beta-1,3-1,4-mixed linked glucans at a single binding site.
The Journal of biological chemistry 2004;279(33):34785-93.
2004: Fernando M V Dias; Florence Vincent; Gavin Pell; José A M Prates; Maria S J Centeno; Louise E Tailford; Luís M A Ferreira; Carlos M G A Fontes; Gideon J Davies; Harry J Gilbert
Insights into the molecular determinants of substrate specificity in glycoside hydrolase family 5 revealed by the crystal structure and kinetics of Cellvibrio mixtus mannosidase 5A.
The Journal of biological chemistry 2004;279(24):25517-26.
2004: Virgínia M R Pires; Joanna L Henshaw; José A M Prates; David N Bolam; Luís M A Ferreira; Carlos M G A Fontes; Bernard Henrissat; Antoni Planas; Harry J Gilbert; Mirjam Czjzek
The crystal structure of the family 6 carbohydrate binding module from Cellvibrio mixtus endoglucanase 5a in complex with oligosaccharides reveals two distinct binding sites with different ligand specificities.
The Journal of biological chemistry 2004;279(20):21560-8.
2004: Joanna L Henshaw; David N Bolam; Virgínia M R Pires; Mirjam Czjzek; Bernard Henrissat; Luís M A Ferreira; Carlos M G A Fontes; Harry J Gilbert
The family 6 carbohydrate binding module CmCBM6-2 contains two ligand-binding sites with distinct specificities.
The Journal of biological chemistry 2004;279(20):21552-9.
2004: P I P Ponte; I Mendes; M Quaresma; M N M Aguiar; J P C Lemos; Luís M A Ferreira; M A C Soares; C M Alfaia; José A M Prates; Carlos M G A Fontes
Cholesterol levels and sensory characteristics of meat from broilers consuming moderate to high levels of alfalfa.
Poultry science 2004;83(5):810-4.
2004: Gavin Pell; Edward J Taylor; Tracey M Gloster; Johan P Turkenburg; Carlos M G A Fontes; Luís M A Ferreira; Tibor Nagy; Samantha J Clark; Gideon J Davies; Harry J Gilbert
The mechanisms by which family 10 glycoside hydrolases bind decorated substrates.
The Journal of biological chemistry 2004;279(10):9597-605.
2003: Ana L Carvalho; Fernando M V Dias; José A M Prates; Tibor Nagy; Harry J Gilbert; Gideon J Davies; Luís M A Ferreira; Maria João Romão; Carlos M G A Fontes
Cellulosome assembly revealed by the crystal structure of the cohesin-dockerin complex.
Proceedings of the National Academy of Sciences of the United States of America 2003;100(24):13809-14.
2003: M M R E Niza; Cristina Lobo Vilela; Luís M A Ferreira
Feline pansteatitis revisited: hazards of unbalanced home-made diets.
Journal of feline medicine and surgery 2003;5(5):271-7.
2002: Tibor Nagy; kaveh emami; Carlos M G A Fontes; Luís M A Ferreira; David R Humphry; Harry J Gilbert
The membrane-bound alpha-glucuronidase from Pseudomonas cellulosa hydrolyzes 4-O-methyl-D-glucuronoxylooligosaccharides but not 4-O-methyl-D-glucuronoxylan.
Journal of bacteriology 2002;184(17):4925-9.
2002: kaveh emami; Tibor Nagy; Carlos M G A Fontes; Luís M A Ferreira; Harry J Gilbert
Evidence for temporal regulation of the two Pseudomonas cellulosa xylanases belonging to glycoside hydrolase family 11.
Journal of bacteriology 2002;184(15):4124-33.
2001: Mirjam Czjzek; David N Bolam; Amor Mosbah; Julie Allouch; Carlos M G A Fontes; Luís M A Ferreira; Olivier Bornet; Véronique Zamboni; Hervé Darbon; Nicola L Smith; Gary W Black; Bernard Henrissat; Harry J Gilbert
The location of the ligand-binding site of carbohydrate-binding modules that have evolved from a common sequence is not conserved.
The Journal of biological chemistry 2001;276(51):48580-7.
2001: José A M Prates; Nicolas Tarbouriech; Simon Charnock; Carlos M G A Fontes; Luís M A Ferreira; Gideon J Davies
The structure of the feruloyl esterase module of xylanase 10B from Clostridium thermocellum provides insights into substrate recognition.
Structure (London, England : 1993) 2001;9(12):1183-90.
2001: Hefang Xie; Harry J Gilbert; Simon Charnock; Gideon J Davies; Michael P Williamson; Pete Simpson; Srinivasarao Raghothama; Carlos M G A Fontes; Fernando M V Dias; Luís M A Ferreira; David N Bolam
Clostridium thermocellum Xyn10B carbohydrate-binding module 22-2: the role of conserved amino acids in ligand binding.
Biochemistry 2001;40(31):9167-76.
2000: Carlos M G A Fontes; Harry J Gilbert; GP Hazlewood; Jonathan H Clarke; José A M Prates; Vincent A McKie; Tibor Nagy; TH Fernandes; Luís M A Ferreira
A novel Cellvibrio mixtus family 10 xylanase that is both intracellular and expressed under non-inducing conditions.
Microbiology (Reading, England) 2000;146 ( Pt 8)():1959-67.
2000: Simon Charnock; David N Bolam; Johan P Turkenburg; Harry J Gilbert; Luís M A Ferreira; Gideon J Davies; Carlos M G A Fontes
The X6 "thermostabilizing" domains of xylanases are carbohydrate-binding modules: structure and biochemistry of the Clostridium thermocellum X6b domain.
Biochemistry 2000;39(17):5013-21.
1999: A C Fernandes; Carlos M G A Fontes; Harry J Gilbert; GP Hazlewood; TH Fernandes; Luís M A Ferreira
Homologous xylanases from Clostridium thermocellum: evidence for bi-functional activity, synergism between xylanase catalytic modules and the presence of xylan-binding domains in enzyme complexes.
The Biochemical journal 1999;342 ( Pt 1)():105-10.
1998: Carlos M G A Fontes; Jonathan H Clarke; GP Hazlewood; TH Fernandes; Harry J Gilbert; Luís M A Ferreira
Identification of tandemly repeated type VI cellulose-binding domains in an endoglucanase from the aerobic soil bacterium Cellvibrio mixtus.
Applied microbiology and biotechnology 1998;49(5):552-9.
1997: Carlos M G A Fontes; Jonathan H Clarke; GP Hazlewood; TH Fernandes; Harry J Gilbert; Luís M A Ferreira
Possible roles for a non-modular, thermostable and proteinase-resistant cellulase from the mesophilic aerobic soil bacterium Cellvibrio mixtus.
Applied microbiology and biotechnology 1997;48(4):473-9.
1997: Gary W Black; JE Rixon; Jonathan H Clarke; GP Hazlewood; Luís M A Ferreira; David N Bolam; Harry J Gilbert
Cellulose binding domains and linker sequences potentiate the activity of hemicellulases against complex substrates.
Journal of biotechnology 1997;57(1-3):59-69.
1995: J Hall; Gary W Black; Luís M A Ferreira; S J Millward-Sadler; bassam rushdi ali; GP Hazlewood; Harry J Gilbert
The non-catalytic cellulose-binding domain of a novel cellulase from Pseudomonas fluorescens subsp. cellulosa is important for the efficient hydrolysis of Avicel.
The Biochemical journal 1995;309 ( Pt 3)():749-56.
1993: Luís M A Ferreira; T M Wood; Gary Williamson; Craig B Faulds; GP Hazlewood; Gary W Black; Harry J Gilbert
A modular esterase from Pseudomonas fluorescens subsp. cellulosa contains a non-catalytic cellulose-binding domain.
The Biochemical journal 1993;294 ( Pt 2)():349-55.
1992: JE Rixon; Luís M A Ferreira; A J Durrant; J I Laurie; GP Hazlewood; Harry J Gilbert
Characterization of the gene celD and its encoded product 1,4-beta-D-glucan glucohydrolase D from Pseudomonas fluorescens subsp. cellulosa.
The Biochemical journal 1992;285 ( Pt 3)():947-55.
1992: GP Hazlewood; J I Laurie; Luís M A Ferreira; Harry J Gilbert
Pseudomonas fluorescens subsp. cellulosa: an alternative model for bacterial cellulase.
The Journal of applied bacteriology 1992;72(3):244-51.
1991: Luís M A Ferreira; GP Hazlewood; P J Barker; Harry J Gilbert
The cellodextrinase from Pseudomonas fluorescens subsp. cellulosa consists of multiple functional domains.
The Biochemical journal 1991;279 ( Pt 3)():793-9.
1990: L E Kellett; D M Poole; Luís M A Ferreira; A J Durrant; GP Hazlewood; Harry J Gilbert
Xylanase B and an arabinofuranosidase from Pseudomonas fluorescens subsp. cellulosa contain identical cellulose-binding domains and are encoded by adjacent genes.
The Biochemical journal 1990;272(2):369-76.
1990: Luís M A Ferreira; A J Durrant; J Hall; GP Hazlewood; Harry J Gilbert
Spatial separation of protein domains is not necessary for catalytic activity or substrate binding in a xylanase.
The Biochemical journal 1990;269(1):261-4.
1990: Harry J Gilbert; J Hall; GP Hazlewood; Luís M A Ferreira
The N-terminal region of an endoglucanase from Pseudomonas fluorescens subspecies cellulosa constitutes a cellulose-binding domain that is distinct from the catalytic centre.
Molecular microbiology 1990;4(5):759-67.