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Carlos Fontes

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Aldino Viegas; Ana L Carvalho; Daniel F Duarte; Carlos M G A Fontes; Filipe Freire; Anjos L Macedo; Maria João Romão; João Sardinha; Eurico J Cabrita
Solution structure, dynamics and binding studies of a family 11 carbohydrate-binding module from Clostridium thermocellum (CtCBM11).
Ana S H Costa; Carlos M G A Fontes; Virgínia M R Pires; Cristina P M Alfaia; R J B Bessa; Marta P Silva; José A M Prates
Genetic background and diet impact beef fatty acid composition and stearoyl-CoA desaturase mRNA expression.
Mark A Currie; Fernando M V Dias; Carlos M G A Fontes; Edward A Bayer; Kate Cameron; Steven P Smith; Holly L Spencer; Zongchao Jia
Small angle X-ray scattering analysis of Clostridium thermocellum cellulosome N-terminal complexes reveals a highly dynamic structure.

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2013: Aldino Viegas; Ana L Carvalho; Daniel F Duarte; Carlos M G A Fontes; Filipe Freire; Anjos L Macedo; Maria João Romão; João Sardinha; Eurico J Cabrita
Solution structure, dynamics and binding studies of a family 11 carbohydrate-binding module from Clostridium thermocellum (CtCBM11).
The Biochemical journal 2013;451(2):289-300.
2013: Ana S H Costa; Carlos M G A Fontes; Virgínia M R Pires; Cristina P M Alfaia; R J B Bessa; Marta P Silva; José A M Prates
Genetic background and diet impact beef fatty acid composition and stearoyl-CoA desaturase mRNA expression.
Lipids 2013;48(4):369-81.
2013: Mark A Currie; Fernando M V Dias; Carlos M G A Fontes; Edward A Bayer; Kate Cameron; Steven P Smith; Holly L Spencer; Zongchao Jia
Small angle X-ray scattering analysis of Clostridium thermocellum cellulosome N-terminal complexes reveals a highly dynamic structure.
The Journal of biological chemistry 2013;288(11):7978-85.
2013: Ana S H Costa; Paulo Costa; Carlos M G A Fontes; José P C Lemos; José Santos-Silva; Jorge A Simões; R J B Bessa; José A M Prates
Carcass fat partitioning and meat quality of Alentejana and Barrosã young bulls fed high or low maize silage diets.
Meat science 2013;93(3):405-12.
2013: Ana S Luís; Shabir Najmudin; José A M Prates; Artur Rogowski; Max J Temple; Immacolata Venditto; Jie Xue; Arnaud Baslé; Luís M A Ferreira; Carlos M G A Fontes; J Paul Knox; Harry J Gilbert
Understanding how noncatalytic carbohydrate binding modules can display specificity for xyloglucan.
The Journal of biological chemistry 2013;288(7):4799-809.
2013: Immacolata Venditto; Max J Temple; Arnaud Baslé; Luís M A Ferreira; Carlos M G A Fontes; Harry J Gilbert; Ana S Luís; Shabir Najmudin
Overproduction, purification, crystallization and preliminary X-ray characterization of the C-terminal family 65 carbohydrate-binding module (CBM65B) of endoglucanase Cel5A from Eubacterium cellulosolvens.
Acta crystallographica. Section F, Structural biology and crystallization communications 2013;69(Pt 2):191-4.
2012: Joana L A Brás; Ana L Carvalho; Luís M A Ferreira; Harry J Gilbert; Shabir Najmudin; José A M Prates; Maria João Romão; Victor D Alves; David N Bolam; Carlos M G A Fontes
Novel Clostridium thermocellum type I cohesin-dockerin complexes reveal a single binding mode.
The Journal of biological chemistry 2012;287(53):44394-405.
2012: Kate Cameron; Luís M A Ferreira; Carlos M G A Fontes; Victor D Alves; Pedro Bule; Shabir Najmudin
Purification, crystallization and preliminary X-ray characterization of the Acetivibrio cellulolyticus type I cohesin ScaC in complex with the ScaB dockerin.
Acta crystallographica. Section F, Structural biology and crystallization communications 2012;68(Pt 9):1030-3.
2012: Kimiya Mizutani; Makiko Sakka; Xiaoyang Zhang; Vânia O Fernandes; Carlos M G A Fontes; Harry J Gilbert; Adam Jackson; Shuichi Karita; Tetsuya Kimura; Ana S Luís; Kazuo Sakka
Influence of a mannan binding family 32 carbohydrate binding module on the activity of the appended mannanase.
Applied and environmental microbiology 2012;78(14):4781-7.
2012: Saprativ P Das; Carlos M G A Fontes; Shadab Ahmed; Debasish Das; Dinesh Goyal; Rajeev Ravindran; Arun Goyal
Bioethanol production involving recombinant C. thermocellum hydrolytic hemicellulase and fermentative microbes.
Applied biochemistry and biotechnology 2012;167(6):1475-88.
2012: Joana L A Brás; Ana Luisa Carvalho; Luís M A Ferreira; Harry J Gilbert; Shabir Najmudin; José A M Prates; Maria João Romão; Aldino Viegas; Victor D Alves; Carlos M G A Fontes
Escherichia coli expression, purification, crystallization, and structure determination of bacterial cohesin-dockerin complexes.
Methods in enzymology 2012;510():395-415.
2012: Ana S H Costa; Marta Estevão; Carlos M G A Fontes; Paula A Lopes; Susana V Martins; Mário Pinho; Rui M A Pinto; Hugo Pissarra; SP Alves; Jorge J Correia; José A M Prates
Contrasting cellularity and fatty acid composition in fat depots from Alentejana and Barrosã bovine breeds fed high and low forage diets.
International journal of biological sciences 2012;8(2):214-27.
2011: Joana L A Brás; Márcia A S Correia; Carlos M G A Fontes; José A M Prates; Maria João Romão; Shabir Najmudin
Purification, crystallization and preliminary X-ray characterization of the pentamodular arabinoxylanase CtXyl5A from Clostridium thermocellum.
Acta crystallographica. Section F, Structural biology and crystallization communications 2011;67(Pt 7):833-6.
2011: Cedric Y Montanier; Samuel J Polizzi; José A M Prates; Yanping Zhu; Arnaud Baslé; Márcia A S Correia; Pedro M Coutinho; James E Flint; Carlos M G A Fontes; Bernard Henrissat; Richard J Lewis; Lauren S McKee; Harry J Gilbert
A novel, noncatalytic carbohydrate-binding module displays specificity for galactose-containing polysaccharides through calcium-mediated oligomerization.
The Journal of biological chemistry 2011;286(25):22499-509.
2011: Márcia A S Correia; Susan J Firbank; Carlos M G A Fontes; Richard J Lewis; Koushik Mazumder; William S York; Yanping Zhu; Joana L A Brás; Harry J Gilbert
Structure and function of an arabinoxylan-specific xylanase.
The Journal of biological chemistry 2011;286(25):22510-20.
2011: T Ribeiro; M M S Lordelo; P I P Ponte; B Maçãs; José A M Prates; M Aguiar Fontes; L Falcão; J P B Freire; L M A Ferreira; Carlos M G A Fontes
Levels of endogenous β-glucanase activity in barley affect the efficacy of exogenous enzymes used to supplement barley-based diets for poultry.
Poultry science 2011;90(6):1245-56.
2011: Begoña García-Alvarez; Roberto Melero; Fernando M V Dias; José A M Prates; Carlos M G A Fontes; Steven P Smith; Maria João Romão; Ana L Carvalho; Oscar Llorca
Molecular architecture and structural transitions of a Clostridium thermocellum mini-cellulosome.
Journal of molecular biology 2011;407(4):571-80.
2011: Ana S Luís; Victor D Alves; Maria João Romão; José A M Prates; Carlos M G A Fontes; Shabir Najmudin
Overproduction, purification, crystallization and preliminary X-ray characterization of a novel carbohydrate-binding module of endoglucanase Cel5A from Eubacterium cellulosolvens.
Acta crystallographica. Section F, Structural biology and crystallization communications 2011;67(Pt 4):491-3.
2011: Joana L A Brás; Alan Cartmell; Ana Luísa M Carvalho; Genny Verzé; Edward A Bayer; Yael Vazana; Márcia A S Correia; José A M Prates; Supriya Ratnaparkhe; Alisdair B Boraston; Maria J Romão; Carlos M G A Fontes; Harry J Gilbert
Structural insights into a unique cellulase fold and mechanism of cellulose hydrolysis.
Proceedings of the National Academy of Sciences of the United States of America 2011;108(13):5237-42.
2010: Shabir Najmudin; Benedita A Pinheiro; José A M Prates; Harry J Gilbert; Maria João Romão; Carlos M G A Fontes
Putting an N-terminal end to the Clostridium thermocellum xylanase Xyn10B story: crystal structure of the CBM22-1-GH10 modules complexed with xylohexaose.
Journal of structural biology 2010;172(3):353-62.
2010: Teresa Ribeiro; Teresa Santos-Silva; Victor D Alves; Fernando M V Dias; Ana S Luís; José A M Prates; Luís M A Ferreira; Maria J Romão; Carlos M G A Fontes
Family 42 carbohydrate-binding modules display multiple arabinoxylan-binding interfaces presenting different ligand affinities.
Biochimica et biophysica acta 2010;1804(10):2054-62.
2010: Márcia A S Correia; D Wade Abbott; Tracey M Gloster; Vânia O Fernandes; José A M Prates; Cedric Montanier; Claire Dumon; Michael P Williamson; Richard B Tunnicliffe; Ziyuan Liu; James E Flint; Gideon J Davies; Bernard Henrissat; Pedro M Coutinho; Carlos M G A Fontes; Harry J Gilbert
Signature active site architectures illuminate the molecular basis for ligand specificity in family 35 carbohydrate binding module.
Biochemistry 2010;49(29):6193-205.
2010: Cristina M M Alfaia; Susana P Alves; Anabela F Lopes; Maria J E Fernandes; Ana S H Costa; Carlos M G A Fontes; Matilde L F Castro; Rui J B Bessa; José A M Prates
Effect of cooking methods on fatty acids, conjugated isomers of linoleic acid and nutritional quality of beef intramuscular fat.
Meat science 2010;84(4):769-77.
2010: Carlos M G A Fontes; Harry J Gilbert
Cellulosomes: highly efficient nanomachines designed to deconstruct plant cell wall complex carbohydrates.
Annual review of biochemistry 2010;79():655-81.
2009: Benedita A Pinheiro; Harry J Gilbert; Kazutaka Sakka; Kazuo Sakka; Vânia O Fernandes; José A M Prates; Victor D Alves; David N Bolam; Luís M A Ferreira; Carlos M G A Fontes
Functional insights into the role of novel type I cohesin and dockerin domains from Clostridium thermocellum.
The Biochemical journal 2009;424(3):375-84.
2009: Márcia A S Correia; Virgínia M R Pires; Harry J Gilbert; David N Bolam; Vânia O Fernandes; Victor D Alves; José A M Prates; Luís M A Ferreira; Carlos M G A Fontes
Family 6 carbohydrate-binding modules display multiple beta1,3-linked glucan-specific binding interfaces.
FEMS microbiology letters 2009;300(1):48-57.
2009: Cedric Montanier; Victoria A Money; Virginia M R Pires; James E Flint; Benedita A Pinheiro; Arun Goyal; José A M Prates; Atsushi Izumi; Henrik Stålbrand; Carl Morland; Alan Cartmell; Katarina Kolenova; Evangelos Topakas; Eleanor J Dodson; David N Bolam; Gideon J Davies; Carlos M G A Fontes; Harry J Gilbert
The active site of a carbohydrate esterase displays divergent catalytic and noncatalytic binding functions.
PLoS biology 2009;7(3):e71.
2009: Cedric Montanier; Alicia Lammerts van Bueren; Claire Dumon; James E Flint; Marcia A Correia; Jose A Prates; Susan J Firbank; Richard J Lewis; Gilles G Grondin; Mariana G Ghinet; Tracey M Gloster; Cecile Herve; J Paul Knox; Brian G Talbot; Johan P Turkenburg; Janne Kerovuo; Ryszard Brzezinski; Carlos M G A Fontes; Gideon J Davies; Alisdair B Boraston; Harry J Gilbert
Evidence that family 35 carbohydrate binding modules display conserved specificity but divergent function.
Proceedings of the National Academy of Sciences of the United States of America 2009;106(9):3065-70.
2009: Shadab Ahmed; Sangeeta Bharali; Carlos M G A Fontes; Avishek Majumder; Ravi Kiran Purama; Arun Goyal
Structural and biochemical properties of lichenase from Clostridium thermocellum.
Indian journal of microbiology 2009;49(1):72-6.
2008: T Ribeiro; P I P Ponte; Catarina I P D Guerreiro; H M Santos; L Falcão; J P B Freire; Luís M A Ferreira; José A M Prates; Carlos M G A Fontes; M M Lordelo
A family 11 carbohydrate-binding module (CBM) improves the efficacy of a recombinant cellulase used to supplement barley-based diets for broilers at lower dosage rates.
British poultry science 2008;49(5):600-8.
2008: Shabir Najmudin; Benedita A Pinheiro; Maria João Romão; José A M Prates; Carlos M G A Fontes
Purification, crystallization and crystallographic analysis of Clostridium thermocellum endo-1,4-beta-D-xylanase 10B in complex with xylohexaose.
Acta crystallographica. Section F, Structural biology and crystallization communications 2008;64(Pt 8):715-8.
2008: Catarina I P D Guerreiro; T Ribeiro; P I P Ponte; M M S Lordelo; L Falcao; J P B Freire; Luís M A Ferreira; José A M Prates; Carlos M G A Fontes
Role of a family 11 carbohydrate-binding module in the function of a recombinant cellulase used to supplement a barley-based diet for broiler chickens.
British poultry science 2008;49(4):446-54.
2008: Márcia A S Correia; José A M Prates; Joana Brás; Carlos M G A Fontes; Joseph A Newman; Richard J Lewis; Harry J Gilbert; James E Flint
Crystal structure of a cellulosomal family 3 carbohydrate esterase from Clostridium thermocellum provides insights into the mechanism of substrate recognition.
Journal of molecular biology 2008;379(1):64-72.
2008: P I P Ponte; M M S Lordelo; Catarina I P D Guerreiro; M C Soares; J L Mourao; J P Crespo; D G Crespo; José A M Prates; Luís M A Ferreira; Carlos M G A Fontes
Crop beta-glucanase activity limits the effectiveness of a recombinant cellulase used to supplement a barley-based feed for free-range broilers.
British poultry science 2008;49(3):347-59.
2008: Aldino Viegas; Natércia F Brás; Nuno Cerqueira; Pedro Alexandrino Fernandes; José A M Prates; Carlos M G A Fontes; Marta Bruix; Maria João Romão; Ana L Carvalho; Maria João Ramos; Anjos L Macedo; Eurico J Cabrita
Molecular determinants of ligand specificity in family 11 carbohydrate binding modules: an NMR, X-ray crystallography and computational chemistry approach.
The FEBS journal 2008;275(10):2524-35.
2008: Catarina I P D Guerreiro; Carlos M G A Fontes; Miguel Gama; Lucília Domingues
Escherichia coli expression and purification of four antimicrobial peptides fused to a family 3 carbohydrate-binding module (CBM) from Clostridium thermocellum.
Protein expression and purification 2008;59(1):161-8.
2008: Victoria A Money; Alan Cartmell; Catarina I P D Guerreiro; Valerie M-A Ducros; Carlos M G A Fontes; Harry J Gilbert; Gideon J Davies
Probing the beta-1,3:1,4 glucanase, CtLic26A, with a thio-oligosaccharide and enzyme variants.
Organic & biomolecular chemistry 2008;6(5):851-3.
2008: P I P Ponte; SP Alves; R J B Bessa; Luís M A Ferreira; LT Gama; J L A Brás; Carlos M G A Fontes; José A M Prates
Influence of pasture intake on the fatty acid composition, and cholesterol, tocopherols, and tocotrienols content in meat from free-range broilers.
Poultry science 2008;87(1):80-8.
2008: P I P Ponte; C M C Rosado; J P Crespo; D G Crespo; J L Mourão; M A Chaveiro-Soares; J L A Brás; I Mendes; LT Gama; José A M Prates; Luís M A Ferreira; Carlos M G A Fontes
Pasture intake improves the performance and meat sensory attributes of free-range broilers.
Poultry science 2008;87(1):71-9.
2007: Cristina M M Alfaia; R J B Bessa; Matilde L F Castro; Carlos M G A Fontes; Paulo J L C Ribeiro; Maria J P Trigo; António J I Alfaia; José A M Prates
Irradiation effect on fatty acid composition and conjugated linoleic acid isomers in frozen lamb meat.
Meat science 2007;77(4):689-95.
2007: Susana V Martins; Paula A Lopes; Cristina M Alfaia; Verónica S Ribeiro; Teresa V Guerreiro; Carlos M G A Fontes; Matilde F Castro; Graça Soveral; José A M Prates
Contents of conjugated linoleic acid isomers in ruminant-derived foods and estimation of their contribution to daily intake in Portugal.
The British journal of nutrition 2007;98(6):1206-13.
2007: Cristina P M Alfaia; SP Alves; R J B Bessa; Matilde L F Castro; Carlos M G A Fontes; Susana I V Martins; Ana P V Portugal; José A M Prates
Influence of slaughter season and muscle type on fatty acid composition, conjugated linoleic acid isomeric distribution and nutritional quality of intramuscular fat in Arouquesa-PDO veal.
Meat science 2007;76(4):787-95.
2007: S Bharali; Carlos M G A Fontes; A Majumder; Ravi Kiran Purama; A Goyal
Functional characterization and mutation analysis of family 11, Carbohydrate-Binding Module (CtCBM11) of cellulosomal bifunctional cellulase from Clostridium thermocellum.
Indian journal of microbiology 2007;47(2):109-18.
2007: Ana L Carvalho; Fernando M V Dias; Tibor Nagy; José A M Prates; Mark R Proctor; Nicola Smith; Edward A Bayer; Gideon J Davies; Luís M A Ferreira; Maria João Romão; Carlos M G A Fontes; Harry J Gilbert
Evidence for a dual binding mode of dockerin modules to cohesins.
Proceedings of the National Academy of Sciences of the United States of America 2007;104(9):3089-94.
2007: Cristina M M Alfaia; SP Alves; R J B Bessa; Matilde L F Castro; Carlos M G A Fontes; Susana I V Martins; Ana P V Portugal; José A M Prates
Effect of slaughter season on fatty acid composition, conjugated linoleic acid isomers and nutritional value of intramuscular fat in Barrosã-PDO veal.
Meat science 2007;75(1):44-52.
2006: Maria S J Centeno; Arun Goyal; José A M Prates; Luís M A Ferreira; Harry J Gilbert; Carlos M G A Fontes
Novel modular enzymes encoded by a cellulase gene cluster in Cellvibrio mixtus.
FEMS microbiology letters 2006;265(1):26-34.
2006: Carlos Martinez-Fleites; Catarina I P D Guerreiro; Martin J Baumann; Edward J Taylor; José A M Prates; Luís M A Ferreira; Carlos M G A Fontes; Harry Brumer; Gideon J Davies
Crystal structures of Clostridium thermocellum xyloglucanase, XGH74A, reveal the structural basis for xyloglucan recognition and degradation.
The Journal of biological chemistry 2006;281(34):24922-33.
2006: Maria S J Centeno; Catarina I P D Guerreiro; Fernando M V Dias; Carl Morland; Louise E Tailford; Arun Goyal; José A M Prates; Luís M A Ferreira; Rui M H Caldeira; Emmanuel F Mongodin; Karen E Nelson; Harry J Gilbert; Carlos M G A Fontes
Galactomannan hydrolysis and mannose metabolism in Cellvibrio mixtus.
FEMS microbiology letters 2006;261(1):123-32.
2006: Edward J Taylor; Tracey M Gloster; Johan P Turkenburg; Florence Vincent; Andrzej M Brzozowski; Claude Dupont; François Shareck; Maria S J Centeno; José A M Prates; Vladimír Puchart; Luís M A Ferreira; Carlos M G A Fontes; Peter Biely; Gideon J Davies
Structure and activity of two metal ion-dependent acetylxylan esterases involved in plant cell wall degradation reveals a close similarity to peptidoglycan deacetylases.
The Journal of biological chemistry 2006;281(16):10968-75.
2006: Shabir Najmudin; Catarina I P D Guerreiro; Ana L Carvalho; José A M Prates; Márcia A S Correia; Victor D Alves; Luís M A Ferreira; Maria João Romão; Harry J Gilbert; David N Bolam; Carlos M G A Fontes
Xyloglucan is recognized by carbohydrate-binding modules that interact with beta-glucan chains.
The Journal of biological chemistry 2006;281(13):8815-28.
2006: C M M Alfaia; R J B Bessa; M L F Castro; Carlos M G A Fontes; M R A Lourenço; S I V Martins; A P V Portugal; M A G Quaresma; V S S Ribeiro; José A M Prates
Fatty acid composition, conjugated linoleic acid isomers and cholesterol in beef from crossbred bullocks intensively produced and from Alentejana purebred bullocks reared according to Carnalentejana-PDO specifications.
Meat science 2006;72(3):425-36.
2005: Shabir Najmudin; Catarina I P D Guerreiro; Luís M A Ferreira; Maria João Romão; Carlos M G A Fontes; José A M Prates
Overexpression, purification and crystallization of the two C-terminal domains of the bifunctional cellulase ctCel9D-Cel44A from Clostridium thermocellum.
Acta crystallographica. Section F, Structural biology and crystallization communications 2005;61(Pt 12):1043-5.
2005: Edward J Taylor; Arun Goyal; Catarina I P D Guerreiro; José A M Prates; Victoria A Money; Natalie Ferry; Carl Morland; Antoni Planas; James A Macdonald; Robert V Stick; Harry J Gilbert; Carlos M G A Fontes; Gideon J Davies
How family 26 glycoside hydrolases orchestrate catalysis on different polysaccharides: structure and activity of a Clostridium thermocellum lichenase, CtLic26A.
The Journal of biological chemistry 2005;280(38):32761-7.
2005: Ana L Carvalho; Virginia M R Pires; Tracey M Gloster; Johan P Turkenburg; José A M Prates; Luís M A Ferreira; Maria João Romão; Gideon J Davies; Carlos M G A Fontes; Harry J Gilbert
Insights into the structural determinants of cohesin-dockerin specificity revealed by the crystal structure of the type II cohesin from Clostridium thermocellum SdbA.
Journal of molecular biology 2005;349(5):909-15.
2005: Nicolas Tarbouriech; José A M Prates; Carlos M G A Fontes; Gideon J Davies
Molecular determinants of substrate specificity in the feruloyl esterase module of xylanase 10B from Clostridium thermocellum.
Acta crystallographica. Section D, Biological crystallography 2005;61(Pt 2):194-7.
2004: Florence Vincent; Tracey M Gloster; James Macdonald; Carl Morland; Robert V Stick; Fernando M V Dias; José A M Prates; Carlos M G A Fontes; Harry J Gilbert; Gideon J Davies
Common inhibition of both beta-glucosidases and beta-mannosidases by isofagomine lactam reflects different conformational itineraries for pyranoside hydrolysis.
Chembiochem : a European journal of chemical biology 2004;5(11):1596-9.
2004: Carlos M G A Fontes; P I P Ponte; T C Reis; MC Soares; LT Gama; Fernando M V Dias; Luís M A Ferreira
A family 6 carbohydrate-binding module potentiates the efficiency of a recombinant xylanase used to supplement cereal-based diets for poultry.
British poultry science 2004;45(5):648-56.
2004: Fernando M V Dias; Arun Goyal; Harry J Gilbert; Luís M A Ferreira; Carlos M G A Fontes
The N-terminal family 22 carbohydrate-binding module of xylanase 10B of Clostridium themocellum is not a thermostabilizing domain.
FEMS microbiology letters 2004;238(1):71-8.
2004: Ana L Carvalho; Arun Goyal; José A M Prates; David N Bolam; Harry J Gilbert; Virgínia M R Pires; Luís M A Ferreira; Antoni Planas; Maria João Romão; Carlos M G A Fontes
The family 11 carbohydrate-binding module of Clostridium thermocellum Lic26A-Cel5E accommodates beta-1,4- and beta-1,3-1,4-mixed linked glucans at a single binding site.
The Journal of biological chemistry 2004;279(33):34785-93.
2004: Fernando M V Dias; Florence Vincent; Gavin Pell; José A M Prates; Maria S J Centeno; Louise E Tailford; Luís M A Ferreira; Carlos M G A Fontes; Gideon J Davies; Harry J Gilbert
Insights into the molecular determinants of substrate specificity in glycoside hydrolase family 5 revealed by the crystal structure and kinetics of Cellvibrio mixtus mannosidase 5A.
The Journal of biological chemistry 2004;279(24):25517-26.
2004: Virgínia M R Pires; Joanna L Henshaw; José A M Prates; David N Bolam; Luís M A Ferreira; Carlos M G A Fontes; Bernard Henrissat; Antoni Planas; Harry J Gilbert; Mirjam Czjzek
The crystal structure of the family 6 carbohydrate binding module from Cellvibrio mixtus endoglucanase 5a in complex with oligosaccharides reveals two distinct binding sites with different ligand specificities.
The Journal of biological chemistry 2004;279(20):21560-8.
2004: Joanna L Henshaw; David N Bolam; Virgínia M R Pires; Mirjam Czjzek; Bernard Henrissat; Luís M A Ferreira; Carlos M G A Fontes; Harry J Gilbert
The family 6 carbohydrate binding module CmCBM6-2 contains two ligand-binding sites with distinct specificities.
The Journal of biological chemistry 2004;279(20):21552-9.
2004: P I P Ponte; I Mendes; M Quaresma; M N M Aguiar; J P C Lemos; Luís M A Ferreira; M A C Soares; C M Alfaia; José A M Prates; Carlos M G A Fontes
Cholesterol levels and sensory characteristics of meat from broilers consuming moderate to high levels of alfalfa.
Poultry science 2004;83(5):810-4.
2004: Gavin Pell; Edward J Taylor; Tracey M Gloster; Johan P Turkenburg; Carlos M G A Fontes; Luís M A Ferreira; Tibor Nagy; Samantha J Clark; Gideon J Davies; Harry J Gilbert
The mechanisms by which family 10 glycoside hydrolases bind decorated substrates.
The Journal of biological chemistry 2004;279(10):9597-605.
2003: Ana L Carvalho; Fernando M V Dias; José A M Prates; Tibor Nagy; Harry J Gilbert; Gideon J Davies; Luís M A Ferreira; Maria João Romão; Carlos M G A Fontes
Cellulosome assembly revealed by the crystal structure of the cohesin-dockerin complex.
Proceedings of the National Academy of Sciences of the United States of America 2003;100(24):13809-14.
2002: Tibor Nagy; kaveh emami; Carlos M G A Fontes; Luís M A Ferreira; David R Humphry; Harry J Gilbert
The membrane-bound alpha-glucuronidase from Pseudomonas cellulosa hydrolyzes 4-O-methyl-D-glucuronoxylooligosaccharides but not 4-O-methyl-D-glucuronoxylan.
Journal of bacteriology 2002;184(17):4925-9.
2002: kaveh emami; Tibor Nagy; Carlos M G A Fontes; Luís M A Ferreira; Harry J Gilbert
Evidence for temporal regulation of the two Pseudomonas cellulosa xylanases belonging to glycoside hydrolase family 11.
Journal of bacteriology 2002;184(15):4124-33.
2001: Mirjam Czjzek; David N Bolam; Amor Mosbah; Julie Allouch; Carlos M G A Fontes; Luís M A Ferreira; Olivier Bornet; Véronique Zamboni; Hervé Darbon; Nicola L Smith; Gary W Black; Bernard Henrissat; Harry J Gilbert
The location of the ligand-binding site of carbohydrate-binding modules that have evolved from a common sequence is not conserved.
The Journal of biological chemistry 2001;276(51):48580-7.
2001: José A M Prates; Nicolas Tarbouriech; Simon Charnock; Carlos M G A Fontes; Luís M A Ferreira; Gideon J Davies
The structure of the feruloyl esterase module of xylanase 10B from Clostridium thermocellum provides insights into substrate recognition.
Structure (London, England : 1993) 2001;9(12):1183-90.
2001: Hefang Xie; Harry J Gilbert; Simon Charnock; Gideon J Davies; Michael P Williamson; Pete Simpson; Srinivasarao Raghothama; Carlos M G A Fontes; Fernando M V Dias; Luís M A Ferreira; David N Bolam
Clostridium thermocellum Xyn10B carbohydrate-binding module 22-2: the role of conserved amino acids in ligand binding.
Biochemistry 2001;40(31):9167-76.
2000: Carlos M G A Fontes; Harry J Gilbert; GP Hazlewood; Jonathan H Clarke; José A M Prates; Vincent A McKie; Tibor Nagy; TH Fernandes; Luís M A Ferreira
A novel Cellvibrio mixtus family 10 xylanase that is both intracellular and expressed under non-inducing conditions.
Microbiology (Reading, England) 2000;146 ( Pt 8)():1959-67.
2000: Simon Charnock; David N Bolam; Johan P Turkenburg; Harry J Gilbert; Luís M A Ferreira; Gideon J Davies; Carlos M G A Fontes
The X6 "thermostabilizing" domains of xylanases are carbohydrate-binding modules: structure and biochemistry of the Clostridium thermocellum X6b domain.
Biochemistry 2000;39(17):5013-21.
1999: A C Fernandes; Carlos M G A Fontes; Harry J Gilbert; GP Hazlewood; TH Fernandes; Luís M A Ferreira
Homologous xylanases from Clostridium thermocellum: evidence for bi-functional activity, synergism between xylanase catalytic modules and the presence of xylan-binding domains in enzyme complexes.
The Biochemical journal 1999;342 ( Pt 1)():105-10.
1999: Carlos M G A Fontes; S Ali; Harry J Gilbert; GP Hazlewood; Barry H Hirst; J Hall
Bacterial xylanase expression in mammalian cells and transgenic mice.
Journal of biotechnology 1999;72(1-2):95-101.
1998: Carlos M G A Fontes; Jonathan H Clarke; GP Hazlewood; TH Fernandes; Harry J Gilbert; Luís M A Ferreira
Identification of tandemly repeated type VI cellulose-binding domains in an endoglucanase from the aerobic soil bacterium Cellvibrio mixtus.
Applied microbiology and biotechnology 1998;49(5):552-9.
1997: S Ali; Carlos M G A Fontes; GP Hazlewood; Barry H Hirst; A J Clark; Harry J Gilbert; J Hall
Co-integration and expression of bacterial and genomic transgenes in the pancreatic and intestinal tissues of transgenic mice.
Gene 1997;202(1-2):203-8.
1997: Carlos M G A Fontes; Jonathan H Clarke; GP Hazlewood; TH Fernandes; Harry J Gilbert; Luís M A Ferreira
Possible roles for a non-modular, thermostable and proteinase-resistant cellulase from the mesophilic aerobic soil bacterium Cellvibrio mixtus.
Applied microbiology and biotechnology 1997;48(4):473-9.
1996: Jonathan H Clarke; K Davidson; Harry J Gilbert; Carlos M G A Fontes; GP Hazlewood
A modular xylanase from mesophilic Cellulomonas fimi contains the same cellulose-binding and thermostabilizing domains as xylanases from thermophilic bacteria.
FEMS microbiology letters 1996;139(1):27-35.
1995: Carlos M G A Fontes; J Hall; Barry H Hirst; GP Hazlewood; Harry J Gilbert
The resistance of cellulases and xylanases to proteolytic inactivation.
Applied microbiology and biotechnology 1995;43(1):52-7.
1995: Carlos M G A Fontes; GP Hazlewood; Ely Morag; J Hall; Barry H Hirst; Harry J Gilbert
Evidence for a general role for non-catalytic thermostabilizing domains in xylanases from thermophilic bacteria.
The Biochemical journal 1995;307 ( Pt 1)():151-8.