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Roland Freudl

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Sandra Scheele; Hendrik Hellmuth; Karl-Heinz Maurer; Dan Oertel; Johannes Bongaerts; Michael Bott; Stefan Evers; Roland Freudl
Secretory production of an FAD cofactor-containing cytosolic enzyme (sorbitol-xylitol oxidase from Streptomyces coelicolor) using the twin-arginine translocation (Tat) pathway of Corynebacterium glutamicum.
Frank Lausberg; Antonia Heyer; Ava Rebecca Chattopadhyay; Lothar Eggeling; Roland Freudl
A tetracycline inducible expression vector for Corynebacterium glutamicum allowing tightly regulable gene expression.
Liuyang Diao; Qilei Dong; Zhaohui Xu; Sheng Yang; Jiahai Zhou; Roland Freudl
Functional implementation of the posttranslational SecB-SecA protein-targeting pathway in Bacillus subtilis.

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All Publications

2013: Sandra Scheele; Hendrik Hellmuth; Karl-Heinz Maurer; Dan Oertel; Johannes Bongaerts; Michael Bott; Stefan Evers; Roland Freudl
Secretory production of an FAD cofactor-containing cytosolic enzyme (sorbitol-xylitol oxidase from Streptomyces coelicolor) using the twin-arginine translocation (Tat) pathway of Corynebacterium glutamicum.
Microbial biotechnology 2013;6(2):202-6.
2012: Frank Lausberg; Antonia Heyer; Ava Rebecca Chattopadhyay; Lothar Eggeling; Roland Freudl
A tetracycline inducible expression vector for Corynebacterium glutamicum allowing tightly regulable gene expression.
Plasmid 2012;68(2):142-7.
2012: Liuyang Diao; Qilei Dong; Zhaohui Xu; Sheng Yang; Jiahai Zhou; Roland Freudl
Functional implementation of the posttranslational SecB-SecA protein-targeting pathway in Bacillus subtilis.
Applied and environmental microbiology 2012;78(3):651-9.
2012: Julia Fröbel; Frank Lausberg; Anne-Sophie Blümmel; Roland Freudl; Patrick Rose; Matthias Müller
Transmembrane insertion of twin-arginine signal peptides is driven by TatC and regulated by TatB.
Nature communications 2012;3():1311.
2012: Peter Rohe; Deepak Venkanna; Roland Freudl; Britta Kleine; Marco Oldiges
An automated workflow for enhancing microbial bioprocess optimization on a novel microbioreactor platform.
Microbial cell factories 2012;11():144.
2012: Frank Lausberg; Matthias Müller; Patrick Rose; Stefan Fleckenstein; Julia Fröbel; Peter Kreutzenbeck; Roland Freudl
Genetic evidence for a tight cooperation of TatB and TatC during productive recognition of twin-arginine (Tat) signal peptides in Escherichia coli.
PloS one 2012;7(6):e39867.
2010: Chao Yang; Cunjiang Song; Roland Freudl; Ashok Mulchandani; Chuanling Qiao
Twin-arginine translocation of methyl parathion hydrolase in Bacillus subtilis.
Environmental science & technology 2010;44(19):7607-12.
2010: Thijs R H M Kouwen; Allan K Nielsen; Emma L Denham; Jean-Yves François Dubois; Ronald Dorenbos; Michael D Rasmussen; Wim J Quax; Roland Freudl; Jan Maarten van Dijl
Contributions of the pre- and pro-regions of a Staphylococcus hyicus lipase to secretion of a heterologous protein by Bacillus subtilis.
Applied and environmental microbiology 2010;76(3):659-69.
2010: Chao Yang; Roland Freudl; Chuanling Qiao; Ashok Mulchandani
Cotranslocation of methyl parathion hydrolase to the periplasm and of organophosphorus hydrolase to the cell surface of Escherichia coli by the Tat pathway and ice nucleation protein display system.
Applied and environmental microbiology 2010;76(2):434-40.
2009: Chao Yang; Roland Freudl; Chuanling Qiao
Export of methyl parathion hydrolase to the periplasm by the twin-arginine translocation pathway in Escherichia coli.
Journal of agricultural and food chemistry 2009;57(19):8901-5.
2009: Daisuke Matsui; Tadao Oikawa; Noriaki Arakawa; Shintaro Osumi; Frank Lausberg; Norma Stäbler; Roland Freudl; Lothar Eggeling
A periplasmic, pyridoxal-5'-phosphate-dependent amino acid racemase in Pseudomonas taetrolens.
Applied microbiology and biotechnology 2009;83(6):1045-54.
2008: Thijs R H M Kouwen; Jean-Yves François Dubois; Roland Freudl; Wim J Quax; Jan Maarten van Dijl
Modulation of thiol-disulfide oxidoreductases for increased production of disulfide-bond-containing proteins in Bacillus subtilis.
Applied and environmental microbiology 2008;74(24):7536-45.
2008: Michael Caspers; Roland Freudl
Corynebacterium glutamicum possesses two secA homologous genes that are essential for viability.
Archives of microbiology 2008;189(6):605-10.
2007: Claire M L Barrett; Roland Freudl; Colin Robinson
Twin arginine translocation (Tat)-dependent export in the apparent absence of TatABC or TatA complexes using modified Escherichia coli TatA subunits that substitute for TatB.
The Journal of biological chemistry 2007;282(50):36206-13.
2007: Daniel Meissner; Angela Vollstedt; Jan Maarten van Dijl; Roland Freudl
Comparative analysis of twin-arginine (Tat)-dependent protein secretion of a heterologous model protein (GFP) in three different Gram-positive bacteria.
Applied microbiology and biotechnology 2007;76(3):633-42.
2007: Peter Kreutzenbeck; Carsten Kröger; Frank Lausberg; Natascha Blaudeck; Georg A Sprenger; Roland Freudl
Escherichia coli twin arginine (Tat) mutant translocases possessing relaxed signal peptide recognition specificities.
The Journal of biological chemistry 2007;282(11):7903-11.
2006: Elise Darmon; Ronald Dorenbos; Jochen Meens; Roland Freudl; Haike Antelmann; Michael Hecker; Oscar P Kuipers; Sierd Bron; Wim J Quax; Jean-Yves François Dubois; Jan Maarten van Dijl
A disulfide bond-containing alkaline phosphatase triggers a BdbC-dependent secretion stress response in Bacillus subtilis.
Applied and environmental microbiology 2006;72(11):6876-85.
2006: Ulf Brockmeier; Michael Caspers; Roland Freudl; Alexander Jockwer; Thomas Noll; Thorsten Eggert
Systematic screening of all signal peptides from Bacillus subtilis: a powerful strategy in optimizing heterologous protein secretion in Gram-positive bacteria.
Journal of molecular biology 2006;362(3):393-402.
2006: Volker Thiemann; Bodo Saake; Angela Vollstedt; Thomas Schäfer; Jürgen Puls; Costanzo Bertoldo; Roland Freudl; Garabed Antranikian
Heterologous expression and characterization of a novel branching enzyme from the thermoalkaliphilic anaerobic bacterium Anaerobranca gottschalkii.
Applied microbiology and biotechnology 2006;72(1):60-71.
2005: Natascha Blaudeck; Peter Kreutzenbeck; Matthias Müller; Georg A Sprenger; Roland Freudl
Isolation and characterization of bifunctional Escherichia coli TatA mutant proteins that allow efficient tat-dependent protein translocation in the absence of TatB.
The Journal of biological chemistry 2005;280(5):3426-32.
2003: Natascha Blaudeck; Peter Kreutzenbeck; Roland Freudl; Georg A Sprenger
Genetic analysis of pathway specificity during posttranslational protein translocation across the Escherichia coli plasma membrane.
Journal of bacteriology 2003;185(9):2811-9.
2001: KH van Wely; Jelto Swaving; Roland Freudl; Arnold J M Driessen
Translocation of proteins across the cell envelope of Gram-positive bacteria.
FEMS microbiology reviews 2001;25(4):437-54.
2001: S Dilsen; W Paul; D Herforth; A Sandgathe; J Altenbach-Rehm; Roland Freudl; Christian Wandrey; Dirk Weuster-Botz
Evaluation of parallel operated small-scale bubble columns for microbial process development using Staphylococcus carnosus.
Journal of biotechnology 2001;88(1):77-84.
2001: Natascha Blaudeck; Georg A Sprenger; Roland Freudl; Thomas Wiegert
Specificity of signal peptide recognition in tat-dependent bacterial protein translocation.
Journal of bacteriology 2001;183(2):604-10.
2000: KH van Wely; Jelto Swaving; M Klein; Roland Freudl; Arnold J M Driessen
The carboxyl terminus of the Bacillus subtilis SecA is dispensable for protein secretion and viability.
Microbiology (Reading, England) 2000;146 ( Pt 10)():2573-81.
2000: S Dilsen; W Paul; A Sandgathe; D Tippe; Roland Freudl; Jörg Thömmes; Maria-Regina Kula; Ralf Takors; Christian Wandrey; Dirk Weuster-Botz
Fed-batch production of recombinant human calcitonin precursor fusion protein using Staphylococcus carnosus as an expression-secretion system.
Applied microbiology and biotechnology 2000;54(3):361-9.
1999: M Vrljic; J Garg; A Bellmann; S Wachi; Roland Freudl; M J Malecki; Hermann Sahm; V J Kozina; Lothar Eggeling; M H Saier; L Eggeling; M H Saier
The LysE superfamily: topology of the lysine exporter LysE of Corynebacterium glutamicum, a paradyme for a novel superfamily of transmembrane solute translocators.
Journal of molecular microbiology and biotechnology 1999;1(2):327-36.
1999: Albert Bolhuis; A Matzen; Hanne-Leena Hyyryläinen; Vesa P Kontinen; Rob Meima; J Chapuis; Gerard Venema; Sierd Bron; Roland Freudl; Jan Maarten van Dijl
Signal peptide peptidase- and ClpP-like proteins of Bacillus subtilis required for efficient translocation and processing of secretory proteins.
The Journal of biological chemistry 1999;274(35):24585-92.
1999: D Halbig; Thomas Wiegert; Natascha Blaudeck; Roland Freudl; Georg A Sprenger
The efficient export of NADP-containing glucose-fructose oxidoreductase to the periplasm of Zymomonas mobilis depends both on an intact twin-arginine motif in the signal peptide and on the generation of a structural export signal induced by cofactor binding.
European journal of biochemistry / FEBS 1999;263(2):543-51.
1999: D Halbig; Bo Hou; Roland Freudl; Georg A Sprenger; Ralf Bernd Klösgen
Bacterial proteins carrying twin-R signal peptides are specifically targeted by the delta pH-dependent transport machinery of the thylakoid membrane system.
FEBS letters 1999;447(1):95-8.
1999: M Herbort; M Klein; E H Manting; Arnold J M Driessen; Roland Freudl
Temporal expression of the Bacillus subtilis secA gene, encoding a central component of the preprotein translocase.
Journal of bacteriology 1999;181(2):493-500.
1997: Jochen Meens; M Herbort; M Klein; Roland Freudl
Use of the pre-pro part of Staphylococcus hyicus lipase as a carrier for secretion of Escherichia coli outer membrane protein A (OmpA) prevents proteolytic degradation of OmpA by cell-associated protease(s) in two different gram-positive bacteria.
Applied and environmental microbiology 1997;63(7):2814-20.
1996: M Klein; Georg A Sprenger; Roland Freudl
Cloning, nucleotide sequence, and functional expression of the Escherichia coli enolase (eno) gene in a temperature-sensitive eno mutant strain.
DNA sequence : the journal of DNA sequencing and mapping 1996;6(6):351-5.
1995: M Klein; J Meens; Roland Freudl
Functional characterization of the Staphylococcus carnosus SecA protein in Escherichia coli and Bacillus subtilis secA mutant strains.
FEMS microbiology letters 1995;131(3):271-7.
1995: JP van der Wolk; M Klose; J G de Wit; Tanneke den Blaauwen; Roland Freudl; Arnold J M Driessen
Identification of the magnesium-binding domain of the high-affinity ATP-binding site of the Bacillus subtilis and Escherichia coli SecA protein.
The Journal of biological chemistry 1995;270(32):18975-82.
1994: M Klein; B Hofmann; M Klose; Roland Freudl
Isolation and characterization of a Bacillus subtilis secA mutant allele conferring resistance to sodium azide.
FEMS microbiology letters 1994;124(3):393-7.
1994: J Meens; M Klose; Roland Freudl
The Staphylococcus carnosus secE gene: cloning, nucleotide sequence, and functional characterization in Escherichia coli secE mutant strains.
FEMS microbiology letters 1994;117(1):113-9.
1993: J Meens; E Frings; M Klose; Roland Freudl
An outer membrane protein (OmpA) of Escherichia coli can be translocated across the cytoplasmic membrane of Bacillus subtilis.
Molecular microbiology 1993;9(4):847-55.
1993: JP van der Wolk; M Klose; Eefjan Breukink; R A Demel; Ben de Kruijff; Roland Freudl; Arnold J M Driessen
Characterization of a Bacillus subtilis SecA mutant protein deficient in translocation ATPase and release from the membrane.
Molecular microbiology 1993;8(1):31-42.
1993: M Klose; K L Schimz; JP van der Wolk; Arnold J M Driessen; Roland Freudl
Lysine 106 of the putative catalytic ATP-binding site of the Bacillus subtilis SecA protein is required for functional complementation of Escherichia coli secA mutants in vivo.
The Journal of biological chemistry 1993;268(6):4504-10.
1992: S Tschauder; A J Driessen; Roland Freudl
Cloning and molecular characterization of the secY genes from Bacillus licheniformis and Staphylococcus carnosus: comparative analysis of nine members of the SecY family.
Molecular & general genetics : MGG 1992;235(1):147-52.
1992: Roland Freudl
Protein secretion in gram-positive bacteria.
Journal of biotechnology 1992;23(3):231-40.
1991: B Overhoff-Freundlieb; Roland Freudl
Suppression of an Escherichia coli secA(ts) mutant by a gene cloned from Staphylococcus carnosus.
FEMS microbiology letters 1991;68(2):143-9.
1991: B Overhoff; M Klein; M Spies; Roland Freudl
Identification of a gene fragment which codes for the 364 amino-terminal amino acid residues of a SecA homologue from Bacillus subtilis: further evidence for the conservation of the protein export apparatus in gram-positive and gram-negative bacteria.
Molecular & general genetics : MGG 1991;228(3):417-23.
1990: Roland Freudl; M Klose; U Henning
Export and sorting of the Escherichia coli outer membrane protein OmpA.
Journal of bioenergetics and biomembranes 1990;22(3):441-9.
1989: Roland Freudl
Insertion of peptides into cell-surface-exposed areas of the Escherichia coli OmpA protein does not interfere with export and membrane assembly.
Gene 1989;82(2):229-36.
1989: Roland Freudl; H Schwarz; M Degen; U Henning
A lower size limit exists for export of fragments of an outer membrane protein (OmpA) of Escherichia coli K-12.
Journal of molecular biology 1989;205(4):771-5.
1988: S MacIntyre; Roland Freudl; M L Eschbach; U Henning
An artificial hydrophobic sequence functions as either an anchor or a signal sequence at only one of two positions within the Escherichia coli outer membrane protein OmpA.
The Journal of biological chemistry 1988;263(35):19053-9.
1988: Roland Freudl; H Schwarz; S Kramps; I Hindennach; U Henning
Dihydrofolate reductase (mouse) and beta-galactosidase (Escherichia coli) can be translocated across the plasma membrane of E. coli.
The Journal of biological chemistry 1988;263(32):17084-91.
1988: Roland Freudl; U Henning
On the role of the mature part of an Escherichia coli outer membrane protein (OmpA) in translocation across the plasma membrane.
Journal of molecular biology 1988;203(2):517-9.
1988: M Klose; H Schwarz; S MacIntyre; Roland Freudl; M L Eschbach; U Henning
Internal deletions in the gene for an Escherichia coli outer membrane protein define an area possibly important for recognition of the outer membrane by this polypeptide.
The Journal of biological chemistry 1988;263(26):13291-6.
1988: Roland Freudl; S MacIntyre; M Degen; U Henning
Alterations to the signal peptide of an outer membrane protein (OmpA) of Escherichia coli K-12 can promote either the cotranslational or the posttranslational mode of processing.
The Journal of biological chemistry 1988;263(1):344-9.
1987: S MacIntyre; Roland Freudl; M Degen; I Hindennach; U Henning
The signal sequence of an Escherichia coli outer membrane protein can mediate translocation of a not normally secreted protein across the plasma membrane.
The Journal of biological chemistry 1987;262(17):8416-22.
1987: Roland Freudl; G Braun; N Honoré; S T Cole
Evolution of the enterobacterial sulA gene: a component of the SOS system encoding an inhibitor of cell division.
Gene 1987;52(1):31-40.
1987: Roland Freudl; H Schwarz; M Degen; U Henning
The signal sequence suffices to direct export of outer membrane protein OmpA of Escherichia coli K-12.
Journal of bacteriology 1987;169(1):66-71.
1986: Roland Freudl; H Schwarz; York-Dieter Stierhof; K Gamon; I Hindennach; U Henning
An outer membrane protein (OmpA) of Escherichia coli K-12 undergoes a conformational change during export.
The Journal of biological chemistry 1986;261(24):11355-61.
1986: Roland Freudl; S MacIntyre; M Degen; U Henning
Cell surface exposure of the outer membrane protein OmpA of Escherichia coli K-12.
Journal of molecular biology 1986;188(3):491-4.
1985: Roland Freudl; H Schwarz; M Klose; N R Movva; U Henning
The nature of information, required for export and sorting, present within the outer membrane protein OmpA of Escherichia coli K-12.
The EMBO journal 1985;4(13A):3593-8.
1985: Roland Freudl; G Braun; I Hindennach; U Henning
Lethal mutations in the structural gene of an outer membrane protein (OmpA) of Escherichia coli K12.
Molecular & general genetics : MGG 1985;201(1):76-81.