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Felix Frolow

Research Profile

Concepts & Ideas

Procedures

X Ray Crystallography

Chemicals & Drugs

Genes & Molecular Sequences

Amino Acid Sequences

Phenomena

Anatomy

Cellulosomes

Living Beings

Clostridium thermocellum

Co-author Network

Publications

TOP 3
Maly Levy-Assaraf; Inna Rozman Grinberg; Linda J W Shimon; Milana Voronov-Goldman; Gloria Weiserman; Bryan A White; Edward A Bayer; Ilya Borovok; Sadanari Jindou; Raphael Lamed; Felix Frolow
Crystal structure of an uncommon cellulosome-related protein module from Ruminococcus flavefaciens that resembles papain-like cysteine peptidases.
Orly Salama-Alber; Linda J W Shimon; Edward A Bayer; Y Gat; Raphael Lamed; Felix Frolow
Crystallization and preliminary X-ray characterization of a type III cohesin-dockerin complex from the cellulosome system of Ruminococcus flavefaciens.
Oren Yaniv; Edward A Bayer; Raphael Lamed; Svetlana Petkun; Linda J W Shimon; Felix Frolow
A single mutation reforms the binding activity of an adhesion-deficient family 3 carbohydrate-binding module.

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All Publications

2013: Maly Levy-Assaraf; Inna Rozman Grinberg; Linda J W Shimon; Milana Voronov-Goldman; Gloria Weiserman; Bryan A White; Edward A Bayer; Ilya Borovok; Sadanari Jindou; Raphael Lamed; Felix Frolow
Crystal structure of an uncommon cellulosome-related protein module from Ruminococcus flavefaciens that resembles papain-like cysteine peptidases.
PloS one 2013;8(2):e56138.
2012: Orly Salama-Alber; Linda J W Shimon; Edward A Bayer; Y Gat; Raphael Lamed; Felix Frolow
Crystallization and preliminary X-ray characterization of a type III cohesin-dockerin complex from the cellulosome system of Ruminococcus flavefaciens.
Acta crystallographica. Section F, Structural biology and crystallization communications 2012;68(Pt 9):1116-9.
2012: Oren Yaniv; Edward A Bayer; Raphael Lamed; Svetlana Petkun; Linda J W Shimon; Felix Frolow
A single mutation reforms the binding activity of an adhesion-deficient family 3 carbohydrate-binding module.
Acta crystallographica. Section D, Biological crystallography 2012;68(Pt 7):819-28.
2012: Oren Yaniv; Felix Frolow; Sadanari Jindou; Raphael Lamed; Edward A Bayer
A simple method for determining specificity of carbohydrate-binding modules for purified and crude insoluble polysaccharide substrates.
Methods in molecular biology (Clifton, N.J.) 2012;908():101-7.
2012: Oren Yaniv; Felix Frolow; Raphael Lamed; Maly Levy-Assraf; Edward A Bayer
Interactions between family 3 carbohydrate binding modules (CBMs) and cellulosomal linker peptides.
Methods in enzymology 2012;510():247-59.
2011: Oren Yaniv; Linda J W Shimon; Edward A Bayer; Raphael Lamed; Felix Frolow
Scaffoldin-borne family 3b carbohydrate-binding module from the cellulosome of Bacteroides cellulosolvens: structural diversity and significance of calcium for carbohydrate binding.
Acta crystallographica. Section D, Biological crystallography 2011;67(Pt 6):506-15.
2011: Milana Voronov-Goldman; Raphael Lamed; Ilit Noach; Ilya Borovok; Moria Kwiat; Sonia Rosenheck; Linda J W Shimon; Edward A Bayer; Felix Frolow
Noncellulosomal cohesin from the hyperthermophilic archaeon Archaeoglobus fulgidus.
Proteins 2011;79(1):50-60.
2011: Noa Cohen-Hadar; Shira Lagziel-Simis; Yariv Wine; Felix Frolow; Amihay Freeman
Re-structuring protein crystals porosity for biotemplating by chemical modification of lysine residues.
Biotechnology and bioengineering 2011;108(1):1-11.
2010: Ilit Noach; Maly Levy-Assaraf; Raphael Lamed; Linda J W Shimon; Felix Frolow; Edward A Bayer
Modular arrangement of a cellulosomal scaffoldin subunit revealed from the crystal structure of a cohesin dyad.
Journal of molecular biology 2010;399(2):294-305.
2010: Edi Goihberg; Moshe Peretz; Shoshana Tel-Or; Orly Dym; Linda Shimon; Felix Frolow; Yigal Burstein
Biochemical and structural properties of chimeras constructed by exchange of cofactor-binding domains in alcohol dehydrogenases from thermophilic and mesophilic microorganisms.
Biochemistry 2010;49(9):1943-53.
2010: Svetlana Petkun; Sadanari Jindou; Linda J W Shimon; Sonia Rosenheck; Edward A Bayer; Raphael Lamed; Felix Frolow
Structure of a family 3b' carbohydrate-binding module from the Cel9V glycoside hydrolase from Clostridium thermocellum: structural diversity and implications for carbohydrate binding.
Acta crystallographica. Section D, Biological crystallography 2010;66(Pt 1):33-43.
2009: Orly Alber; Ilit Noach; Marco T Rincon; Harry J Flint; Linda J W Shimon; Raphael Lamed; Felix Frolow; Edward A Bayer
Cohesin diversity revealed by the crystal structure of the anchoring cohesin from Ruminococcus flavefaciens.
Proteins 2009;77(3):699-709.
2009: Yariv Wine; Noa Cohen-Hadar; Raphael Lamed; Amihay Freeman; Felix Frolow
Modification of protein crystal packing by systematic mutations of surface residues: implications on biotemplating and crystal porosity.
Biotechnology and bioengineering 2009;104(3):444-57.
2009: Ilit Noach; Felix Frolow; Orly Alber; Raphael Lamed; Linda J W Shimon; Edward A Bayer
Intermodular linker flexibility revealed from crystal structures of adjacent cellulosomal cohesins of Acetivibrio cellulolyticus.
Journal of molecular biology 2009;391(1):86-97.
2009: Tal Burstein; Michal Shulman; Sadanari Jindou; Svetlana Petkun; Felix Frolow; Yuval Shoham; Edward A Bayer; Raphael Lamed
Physical association of the catalytic and helper modules of a family-9 glycoside hydrolase is essential for activity.
FEBS letters 2009;583(5):879-84.
2009: Milana Voronov-Goldman; Ilit Noach; Raphael Lamed; Linda J W Shimon; Ilya Borovok; Edward A Bayer; Felix Frolow
Crystallization and preliminary X-ray analysis of a cohesin-like module from AF2375 of the archaeon Archaeoglobus fulgidus.
Acta crystallographica. Section F, Structural biology and crystallization communications 2009;65(Pt 3):275-8.
2009: Oded Suad; Haim Rozenberg; Ran Brosh; Yael Diskin-Posner; Naama Kessler; Linda J W Shimon; Felix Frolow; Atar Liran; Varda Rotter; Zippora Shakked
Structural basis of restoring sequence-specific DNA binding and transactivation to mutant p53 by suppressor mutations.
Journal of molecular biology 2009;385(1):249-65.
2009: Lev Weiner; Irina Shin; Linda J W Shimon; Talia Miron; Meir Wilchek; David Mirelman; Felix Frolow; Aharon Rabinkov
Thiol-disulfide organization in alliin lyase (alliinase) from garlic (Allium sativum).
Protein science : a publication of the Protein Society 2009;18(1):196-205.
2008: Edi Goihberg; Orly Dym; Shoshana Tel-Or; Linda J W Shimon; Felix Frolow; Moshe Peretz; Yigal Burstein
Thermal stabilization of the protozoan Entamoeba histolytica alcohol dehydrogenase by a single proline substitution.
Proteins 2008;72(2):711-9.
2008: Orly Alber; Ilit Noach; Raphael Lamed; Linda J W Shimon; Edward A Bayer; Felix Frolow
Preliminary X-ray characterization of a novel type of anchoring cohesin from the cellulosome of Ruminococcus flavefaciens.
Acta crystallographica. Section F, Structural biology and crystallization communications 2008;64(Pt 2):77-80.
2008: Ilit Noach; Orly Alber; Edward A Bayer; Raphael Lamed; Maly Levy-Assaraf; Linda J W Shimon; Felix Frolow
Crystallization and preliminary X-ray analysis of Acetivibrio cellulolyticus cellulosomal type II cohesin module: two versions having different linker lengths.
Acta crystallographica. Section F, Structural biology and crystallization communications 2008;64(Pt 1):58-61.
2007: Sadanari Jindou; Svetlana Petkun; Linda J W Shimon; Edward A Bayer; Raphael Lamed; Felix Frolow
Crystallization and preliminary diffraction studies of CBM3b of cellobiohydrolase 9A from Clostridium thermocellum.
Acta crystallographica. Section F, Structural biology and crystallization communications 2007;63(Pt 12):1044-7.
2007: Yariv Wine; Noa Cohen-Hadar; Amihay Freeman; Felix Frolow
Elucidation of the mechanism and end products of glutaraldehyde crosslinking reaction by X-ray structure analysis.
Biotechnology and bioengineering 2007;98(3):711-8.
2007: Linda J W Shimon; Aharon Rabinkov; Irina Shin; Talia Miron; David Mirelman; Meir Wilchek; Felix Frolow
Two structures of alliinase from Alliium sativum L.: apo form and ternary complex with aminoacrylate reaction intermediate covalently bound to the PLP cofactor.
Journal of molecular biology 2007;366(2):611-25.
2007: Izhar Karbat; Michael Turkov; Lior Cohen; Roy Kahn; Dalia Gordon; Michael Gurevitz; Felix Frolow
X-ray structure and mutagenesis of the scorpion depressant toxin LqhIT2 reveals key determinants crucial for activity and anti-insect selectivity.
Journal of molecular biology 2007;366(2):586-601.
2006: Noa Cohen-Hadar; Yariv Wine; Esther Nachliel; Dan Huppert; Menahem Gutman; Felix Frolow; Amihay Freeman
Monitoring the stability of crosslinked protein crystals biotemplates: a feasibility study.
Biotechnology and bioengineering 2006;94(5):1005-11.
2006: Linda J W Shimon; Edi Goihberg; Moshe Peretz; Yigal Burstein; Felix Frolow
Structure of alcohol dehydrogenase from Entamoeba histolytica.
Acta crystallographica. Section D, Biological crystallography 2006;62(Pt 5):541-7.
2005: Ilit Noach; Felix Frolow; Hilla Jakoby; Sonia Rosenheck; Lindaj W Shimon; Raphael Lamed; Edward A Bayer
Crystal structure of a type-II cohesin module from the Bacteroides cellulosolvens cellulosome reveals novel and distinctive secondary structural elements.
Journal of molecular biology 2005;348(1):1-12.
2004: Omri Drory; Felix Frolow; Nathan Nelson
Crystal structure of yeast V-ATPase subunit C reveals its stator function.
EMBO reports 2004;5(12):1148-52.
2004: Omri Drory; Adi Mor; Felix Frolow; Nathan Nelson
Expression, crystallization and phasing of vacuolar H(+)-ATPase subunit C (Vma5p) of Saccharomyces cerevisiae.
Acta crystallographica. Section D, Biological crystallography 2004;60(Pt 10):1906-9.
2004: Izhar Karbat; Felix Frolow; Oren Froy; Nicolas Gilles; Lior Cohen; Michael Turkov; Dalia Gordon; Michael Gurevitz
Molecular basis of the high insecticidal potency of scorpion alpha-toxins.
The Journal of biological chemistry 2004;279(30):31679-86.
2004: Inna Levin; Gal Meiri; Moshe Peretz; Yigal Burstein; Felix Frolow
The ternary complex of Pseudomonas aeruginosa alcohol dehydrogenase with NADH and ethylene glycol.
Protein science : a publication of the Protein Society 2004;13(6):1547-56.
2004: Adam Ben-Shem; Felix Frolow; Nathan Nelson
Evolution of photosystem I - from symmetry through pseudo-symmetry to asymmetry.
FEBS letters 2004;564(3):274-80.
2003: Adam Ben-Shem; Felix Frolow; Nathan Nelson
Crystal structure of plant photosystem I.
Nature 2003;426(6967):630-5.
2003: Adam Ben-Shem; Nathan Nelson; Felix Frolow
Crystallization and initial X-ray diffraction studies of higher plant photosystem I.
Acta crystallographica. Section D, Biological crystallography 2003;59(Pt 10):1824-7.
2003: Ilit Noach; Raphael Lamed; Qi Xu; Sonia Rosenheck; Linda J W Shimon; Edward A Bayer; Felix Frolow
Preliminary X-ray characterization and phasing of a type II cohesin domain from the cellulosome of Acetivibrio cellulolyticus.
Acta crystallographica. Section D, Biological crystallography 2003;59(Pt 9):1670-3.
2003: Merav Geva; Felix Frolow; Miriam Eisenstein; Lia Addadi
Antibody recognition of chiral surfaces. Enantiomorphous crystals of leucine-leucine-tyrosine.
Journal of the American Chemical Society 2003;125(3):696-704.
2002: Oren Bogin; Inna Levin; Yael Hacham; Shoshana Tel-Or; Moshe Peretz; Felix Frolow; Yigal Burstein
Structural basis for the enhanced thermal stability of alcohol dehydrogenase mutants from the mesophilic bacterium Clostridium beijerinckii: contribution of salt bridging.
Protein science : a publication of the Protein Society 2002;11(11):2561-74.
2002: Deborah Bartfeld; Linda J W Shimon; Graeme C Couture; Dov Rabinovich; Felix Frolow; Ditsa Levanon; Yoram Groner; Zippora Shakked
DNA recognition by the RUNX1 transcription factor is mediated by an allosteric transition in the RUNT domain and by DNA bending.
Structure (London, England : 1993) 2002;10(10):1395-407.
2002: Linda J W Shimon; Aharon Rabinkov; Talia Miron; David Mirelman; Meir Wilchek; Felix Frolow
Alliin lyase (alliinase) from garlic (Allium sativum): crystallization and preliminary X-ray characterization.
Acta crystallographica. Section D, Biological crystallography 2002;58(Pt 8):1335-7.
2002: Linda J W Shimon; Moshe Peretz; Edi Goihberg; Yigal Burstein; Felix Frolow
Thermophilic alcohol dehydrogenase from the mesophile Entamoeba histolytica: crystallization and preliminary X-ray characterization.
Acta crystallographica. Section D, Biological crystallography 2002;58(Pt 3):546-8.
2001: J Hizver; Haim Rozenberg; Felix Frolow; Dov Rabinovich; Zippora Shakked
DNA bending by an adenine--thymine tract and its role in gene regulation.
Proceedings of the National Academy of Sciences of the United States of America 2001;98(15):8490-5.
2000: Linda J W Shimon; Sandrine Pagès; Anne Belaich; Jean-Pierre Belaich; Edward A Bayer; Raphael Lamed; Yuval Shoham; Felix Frolow
Structure of a family IIIa scaffoldin CBD from the cellulosome of Clostridium cellulolyticum at 2.2 A resolution.
Acta crystallographica. Section D, Biological crystallography 2000;56(Pt 12):1560-8.
2000: Moshe Mevarech; Felix Frolow; Lisa M Gloss
Halophilic enzymes: proteins with a grain of salt.
Biophysical chemistry 2000;86(2-3):155-64.
2000: Racheli Kreisberg; Ilya Borovok; Michaela Yanko; Felix Frolow; Yair Aharonowitz; Gerald Cohen
Structure-function studies of the non-heme iron active site of isopenicillin N synthase: some implications for catalysis.
Biophysical chemistry 2000;86(2-3):109-18.
1999: Y Korkhin; A J Kalb (Gilboa); Moshe Peretz; Oren Bogin; Yigal Burstein; Felix Frolow
Oligomeric integrity--the structural key to thermal stability in bacterial alcohol dehydrogenases.
Protein science : a publication of the Protein Society 1999;8(6):1241-9.
1998: Haim Rozenberg; Dov Rabinovich; Felix Frolow; Ramanujan S Hegde; Zippora Shakked
Structural code for DNA recognition revealed in crystal structures of papillomavirus E2-DNA targets.
Proceedings of the National Academy of Sciences of the United States of America 1998;95(26):15194-9.
1998: Y Korkhin; A J Kalb(Gilboa); Moshe Peretz; Oren Bogin; Yigal Burstein; Felix Frolow
NADP-dependent bacterial alcohol dehydrogenases: crystal structure, cofactor-binding and cofactor specificity of the ADHs of Clostridium beijerinckii and Thermoanaerobacter brockii.
Journal of molecular biology 1998;278(5):967-81.
1998: Oren Bogin; Moshe Peretz; Yael Hacham; Y Korkhin; Felix Frolow; A J Kalb(Gilboa); Yigal Burstein
Enhanced thermal stability of Clostridium beijerinckii alcohol dehydrogenase after strategic substitution of amino acid residues with prolines from the homologous thermophilic Thermoanaerobacter brockii alcohol dehydrogenase.
Protein science : a publication of the Protein Society 1998;7(5):1156-63.
1997: Linda J W Shimon; Edward A Bayer; Ely Morag; Raphael Lamed; Sima Yaron; Yuval Shoham; Felix Frolow
A cohesin domain from Clostridium thermocellum: the crystal structure provides new insights into cellulosome assembly.
Structure (London, England : 1993) 1997;5(3):381-90.
1997: Linda J W Shimon; Felix Frolow; Sima Yaron; Edward A Bayer; Raphael Lamed; Ely Morag; Yuval Shoham
Crystallization and preliminary X-ray analysis of a cohesin domain of the cellulosome from Clostridium thermocellum.
Acta crystallographica. Section D, Biological crystallography 1997;53(Pt 1):114-5.
1996: Sima Yaron; Linda J W Shimon; Felix Frolow; Raphael Lamed; Ely Morag; Yuval Shoham; Edward A Bayer
Expression, purification and crystallization of a cohesin domain from the cellulosome of Clostridium thermocellum.
Journal of biotechnology 1996;51(3):243-9.
1996: Y Korkhin; Felix Frolow; Oren Bogin; Moshe Peretz; A J Kalb; Yigal Burstein
Crystalline alcohol dehydrogenases from the mesophilic bacterium Clostridium beijerinckii and the thermophilic bacterium Thermoanaerobium brockii: preparation, characterization and molecular symmetry.
Acta crystallographica. Section D, Biological crystallography 1996;52(Pt 4):882-6.
1996: Felix Frolow; Michal Harel; Joel Sussman; M Mevarech; Menachem Shoham
Insights into protein adaptation to a saturated salt environment from the crystal structure of a halophilic 2Fe-2S ferredoxin.
Nature structural biology 1996;3(5):452-8.
1995: A J Kalb; Felix Frolow; J Yariv
Properties of a crystal of the complex of methyl D-arabinofuranoside with concanavalin A.
Acta crystallographica. Section D, Biological crystallography 1995;51(Pt 6):1077-9.
1994: Felix Frolow; A J Kalb; J Yariv
Structure of a unique twofold symmetric haem-binding site.
Nature structural biology 1994;1(7):453-60.
1994: Zippora Shakked; G Guzikevich-Guerstein; Felix Frolow; Dov Rabinovich; A Joachimiak; P B Sigler
Determinants of repressor/operator recognition from the structure of the trp operator binding site.
Nature 1994;368(6470):469-73.
1993: Felix Frolow; A J Kalb; J Yariv
Location of haem in bacterioferritin of E. coli.
Acta crystallographica. Section D, Biological crystallography 1993;49(Pt 6):597-600.
1993: L Inbar; Felix Frolow; Aviva Lapidot
The conformation of new tetrahydropyrimidine derivatives in solution and in the crystal.
European journal of biochemistry / FEBS 1993;214(3):897-906.
1993: U G Wagner; Katherine A Pattridge; Martha L Ludwig; W C Stallings; M M Werber; C Oefner; Felix Frolow; Joel Sussman
Comparison of the crystal structures of genetically engineered human manganese superoxide dismutase and manganese superoxide dismutase from Thermus thermophilus: differences in dimer-dimer interaction.
Protein science : a publication of the Protein Society 1993;2(5):814-25.
1993: Z Zhang; A Djebli; Menachem Shoham; Felix Frolow; Moshe Peretz; Yigal Burstein
Crystal parameters of an alcohol dehydrogenase from the extreme thermophile Thermoanaerobium brockii.
Journal of molecular biology 1993;230(1):353-5.
1992: Leemor Joshua-Tor; Felix Frolow; E Appella; H Hope; Dov Rabinovich; Joel Sussman
Three-dimensional structures of bulge-containing DNA fragments.
Journal of molecular biology 1992;225(2):397-431.
1992: David L Ollis; E Cheah; Miroslaw Cygler; B Dijkstra; Felix Frolow; S M Franken; Michal Harel; S J Remington; Israel Silman; Joseph D Schrag
The alpha/beta hydrolase fold.
Protein engineering 1992;5(3):197-211.
1992: J Moradian-Oldak; Felix Frolow; Lia Addadi; Steve Weiner
Interactions between acidic matrix macromolecules and calcium phosphate ester crystals: relevance to carbonate apatite formation in biomineralization.
Proceedings. Biological sciences / The Royal Society 1992;247(1318):47-55.
1991: Michal Harel; C T Su; Felix Frolow; Yacov Ashani; Israel Silman; Joel Sussman
Refined crystal structures of "aged" and "non-aged" organophosphoryl conjugates of gamma-chymotrypsin.
Journal of molecular biology 1991;221(3):909-18.
1991: Joel Sussman; Michal Harel; Felix Frolow; C Oefner; A Goldman; L Toker; Israel Silman
Atomic structure of acetylcholinesterase from Torpedo californica: a prototypic acetylcholine-binding protein.
Science (New York, N.Y.) 1991;253(5022):872-9.
1991: Michal Harel; C T Su; Felix Frolow; Israel Silman; Joel Sussman
Gamma-chymotrypsin is a complex of alpha-chymotrypsin with its own autolysis products.
Biochemistry 1991;30(21):5217-25.
1990: Felix Frolow; Menachem Shoham
Crystallization and preliminary x-ray investigation of colicin E3 in complex with its immunity protein.
The Journal of biological chemistry 1990;265(18):10196-7.
1990: Miriam Eisenstein; Felix Frolow; Zippora Shakked; Dov Rabinovich
The structure and hydration of the A-DNA fragment d(GGGTACCC) at room temperature and low temperature.
Nucleic acids research 1990;18(11):3185-94.
1989: Zippora Shakked; G Guerstein-Guzikevich; Miriam Eisenstein; Felix Frolow; Dov Rabinovich
The conformation of the DNA double helix in the crystal is dependent on its environment.
Nature 1989;342(6248):456-60.
1989: U G Wagner; M M Werber; Y Beck; J R Hartman; Felix Frolow; Joel Sussman
Characterization of crystals of genetically engineered human manganese superoxide dismutase.
Journal of molecular biology 1989;206(4):787-8.
1989: H Hope; Felix Frolow; K von Böhlen; I Makowski; C Kratky; Y Halfon; H Danz; P Webster; K S Bartels; H G Wittmann
Cryocrystallography of ribosomal particles.
Acta crystallographica. Section B, Structural science 1989;45 ( Pt 2)():190-9.
1988: Miriam Eisenstein; H Hope; Tali E Haran; Felix Frolow; Zippora Shakked; Dov Rabinovich
Low-temperature study of the A-DNA fragment d(GGGCGCCC).
Acta crystallographica. Section B, Structural science 1988;44 ( Pt 6)():625-8.
1988: Joel Sussman; Michal Harel; Felix Frolow; L Varon; L Toker; A H Futerman; Israel Silman
Purification and crystallization of a dimeric form of acetylcholinesterase from Torpedo californica subsequent to solubilization with phosphatidylinositol-specific phospholipase C.
Journal of molecular biology 1988;203(3):821-3.
1988: Leemor Joshua-Tor; Dov Rabinovich; H Hope; Felix Frolow; E Appella; Joel Sussman
The three-dimensional structure of a DNA duplex containing looped-out bases.
Nature 1988;334(6177):82-4.
1988: Michal Harel; Menachem Shoham; Felix Frolow; H Eisenberg; M Mevarech; Ada Yonath; Joel Sussman
Crystallization of halophilic malate dehydrogenase from Halobacterium marismortui.
Journal of molecular biology 1988;200(3):609-10.
1987: I Makowski; Felix Frolow; Mark Saper; Menachem Shoham; H G Wittmann; Ada Yonath
Single crystals of large ribosomal particles from Halobacterium marismortui diffract to 6 A.
Journal of molecular biology 1987;193(4):819-22.
1986: Ada Yonath; Mark Saper; Felix Frolow; I Makowski; H G Wittmann
Characterization of single crystals of the large ribosomal particles from a mutant of Bacillus stearothermophilus.
Journal of molecular biology 1986;192(1):161-2.