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Akikazu Fujita

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Akikazu Fujita; Nami Ozato-Sakurai; Toyoshi Fujimoto; Hitomi Suzuki
A method for efficient observation of intracellular membranes of monolayer culture cells by quick-freeze and freeze-fracture electron microscopy.
Nami Ozato-Sakurai; Akikazu Fujita; Toyoshi Fujimoto
The distribution of phosphatidylinositol 4,5-bisphosphate in acinar cells of rat pancreas revealed with the freeze-fracture replica labeling method.
Akikazu Fujita; Jinglei Cheng; Toyoshi Fujimoto
Quantitative electron microscopy for the nanoscale analysis of membrane lipid distribution.

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2012: Akikazu Fujita; Nami Ozato-Sakurai; Toyoshi Fujimoto; Hitomi Suzuki
A method for efficient observation of intracellular membranes of monolayer culture cells by quick-freeze and freeze-fracture electron microscopy.
Journal of electron microscopy 2012;61(6):441-6.
2011: Nami Ozato-Sakurai; Akikazu Fujita; Toyoshi Fujimoto
The distribution of phosphatidylinositol 4,5-bisphosphate in acinar cells of rat pancreas revealed with the freeze-fracture replica labeling method.
PloS one 2011;6(8):e23567.
2010: Akikazu Fujita; Jinglei Cheng; Toyoshi Fujimoto
Quantitative electron microscopy for the nanoscale analysis of membrane lipid distribution.
Nature protocols 2010;5(4):661-9.
2010: Akikazu Fujita; Toyoshi Fujimoto
High-resolution molecular localization by freeze-fracture replica labeling.
Methods in molecular biology (Clifton, N.J.) 2010;657():205-16.
2009: Jinglei Cheng; Akikazu Fujita; Yuki Ohsaki; Michitaka Suzuki; Yuki Shinohara; Toyoshi Fujimoto
Quantitative electron microscopy shows uniform incorporation of triglycerides into existing lipid droplets.
Histochemistry and cell biology 2009;132(3):281-91.
2009: Akikazu Fujita; Jinglei Cheng; Kumi Tauchi-Sato; Tadaomi Takenawa; Toyoshi Fujimoto
A distinct pool of phosphatidylinositol 4,5-bisphosphate in caveolae revealed by a nanoscale labeling technique.
Proceedings of the National Academy of Sciences of the United States of America 2009;106(23):9256-61.
2009: Yuki Ohsaki; Jinglei Cheng; Michitaka Suzuki; Yuki Shinohara; Akikazu Fujita; Toyoshi Fujimoto
Biogenesis of cytoplasmic lipid droplets: from the lipid ester globule in the membrane to the visible structure.
Biochimica et biophysica acta 2009;1791(6):399-407.
2009: Akikazu Fujita; Jinglei Cheng; Toyoshi Fujimoto
Segregation of GM1 and GM3 clusters in the cell membrane depends on the intact actin cytoskeleton.
Biochimica et biophysica acta 2009;1791(5):388-96.
2008: Yuki Ohsaki; Jinglei Cheng; Michitaka Suzuki; Akikazu Fujita; Toyoshi Fujimoto
Lipid droplets are arrested in the ER membrane by tight binding of lipidated apolipoprotein B-100.
Journal of cell science 2008;121(Pt 14):2415-22.
2007: Akikazu Fujita; Toyoshi Fujimoto
Quantitative retention of membrane lipids in the freeze-fracture replica.
Histochemistry and cell biology 2007;128(5):385-9.
2007: Eiko Tsuiki; Akikazu Fujita; Yuki Ohsaki; Jinglei Cheng; Toshiaki Irie; Kiwamu Yoshikawa; Haruki Senoo; Kazuaki Mishima; Takashi Kitaoka; Toyoshi Fujimoto
All-trans-retinol generated by rhodopsin photobleaching induces rapid recruitment of TIP47 to lipid droplets in the retinal pigment epithelium.
Investigative ophthalmology & visual science 2007;48(6):2858-67.
2007: Akikazu Fujita; Jinglei Cheng; Minako Hirakawa; Koichi Furukawa; Susumu Kusunoki; Toyoshi Fujimoto
Gangliosides GM1 and GM3 in the living cell membrane form clusters susceptible to cholesterol depletion and chilling.
Molecular biology of the cell 2007;18(6):2112-22.
2006: Jinglei Cheng; Yuki Ohsaki; Kumi Tauchi-Sato; Akikazu Fujita; Toyoshi Fujimoto
Cholesterol depletion induces autophagy.
Biochemical and biophysical research communications 2006;351(1):246-52.
2006: Yuki Ohsaki; Jinglei Cheng; Akikazu Fujita; Toshinobu Tokumoto; Toyoshi Fujimoto
Cytoplasmic lipid droplets are sites of convergence of proteasomal and autophagic degradation of apolipoprotein B.
Molecular biology of the cell 2006;17(6):2674-83.