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Kunio Fujiwara

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Kunio Fujiwara; Tsubasa Miyazaki; Tetsuya Saita; Masashi Shin; Yohei Yoshizaki; Shuichi Nagata
Immunocytochemistry for vancomycin using a monoclonal antibody that reveals accumulation of the drug in rat kidney and liver.
Kunio Fujiwara; Tsubasa Miyazaki; Masashi Shin; Yohei Yoshizaki; Tetsuya Saita
An in vivo role of Mrp2 in the rat hepatocytes by immunocytochemistry for amoxicillin using the transporter-deficient EHBR.
Kunio Fujiwara; Tsubasa Miyazaki; Masashi Shin; Yohei Yoshizaki; Tetsuya Saita
Immunocytochemistry for bestatin and its application to drug accumulation studies in rat intestine and kidney.

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All Publications

2012: Kunio Fujiwara; Tsubasa Miyazaki; Tetsuya Saita; Masashi Shin; Yohei Yoshizaki; Shuichi Nagata
Immunocytochemistry for vancomycin using a monoclonal antibody that reveals accumulation of the drug in rat kidney and liver.
Antimicrobial agents and chemotherapy 2012;56(11):5883-91.
2012: Kunio Fujiwara; Tsubasa Miyazaki; Masashi Shin; Yohei Yoshizaki; Tetsuya Saita
An in vivo role of Mrp2 in the rat hepatocytes by immunocytochemistry for amoxicillin using the transporter-deficient EHBR.
Journal of molecular histology 2012;43(3):371-8.
2011: Kunio Fujiwara; Tsubasa Miyazaki; Masashi Shin; Yohei Yoshizaki; Tetsuya Saita
Immunocytochemistry for bestatin and its application to drug accumulation studies in rat intestine and kidney.
Journal of molecular histology 2011;42(6):589-96.
2011: Kunio Fujiwara; Masashi Shin; Tsubasa Miyazaki
A probable relationship between characteristic accumulation of doxorubicin and P-glycoprotein transporter in rat liver.
Journal of molecular histology 2011;42(5):409-15.
2011: Kunio Fujiwara
[Distribution and accumulation of antibiotics in cells and tissues and toxicity studies by immunocytochemistry].
Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan 2011;131(6):949-60.
2011: Kunio Fujiwara; Masashi Shin; David M Hougaard; Tetsuya Saita
Distribution study of peplomycin in rat kidney revealed by immunocytochemistry using monoclonal antibodies.
Histochemistry and cell biology 2011;135(1):93-101.
2011: Kunio Fujiwara; Masashi Shin; Tsubasa Miyazaki; Yasuhiro Maruta
Immunocytochemistry for amoxicillin and its use for studying uptake of the drug in the intestine, liver, and kidney of rats.
Antimicrobial agents and chemotherapy 2011;55(1):62-71.
2010: Masashi Shin; Hayato Matsunaga; Kunio Fujiwara
Differences in accumulation of anthracyclines daunorubicin, doxorubicin and epirubicin in rat tissues revealed by immunocytochemistry.
Histochemistry and cell biology 2010;133(6):677-82.
2009: Masashi Shin; Lars-Inge Larsson; David M Hougaard; Kunio Fujiwara
Daunomycin accumulation and induction of programmed cell death in rat hair follicles.
Cell and tissue research 2009;337(3):429-38.
2009: Kunio Fujiwara; Masashi Shin; Hayato Matsunaga; Tetsuya Saita; Lars-Inge Larsson
Light-microscopic immunocytochemistry for gentamicin and its use for studying uptake of the drug in kidney.
Antimicrobial agents and chemotherapy 2009;53(8):3302-7.
2008: Masashi Shin; Hiromichi Nakamuta; Naoko Oda-Ueda; Lars-Inge Larsson; Kunio Fujiwara
Immunocytochemical demonstration of polyamines in nucleoli and nuclei.
Histochemistry and cell biology 2008;129(5):659-65.
2007: Koji Ohara; Masashi Shin; Hiromichi Nakamuta; Lars-Inge Larsson; David M Hougaard; Kunio Fujiwara
Immunocytochemical studies on the distribution pattern of daunomycin in rat gastrointestinal tract.
Histochemistry and cell biology 2007;128(3):285-90.
2007: Masashi Shin; Lars-Inge Larsson; Kunio Fujiwara
Polyamines in spermatocytes and residual bodies of rat testis.
Histochemistry and cell biology 2007;127(6):649-55.
2007: Koji Ohara; Masashi Shin; Lars-Inge Larsson; Kunio Fujiwara
Improved immunocytochemical detection of daunomycin.
Histochemistry and cell biology 2007;127(6):603-8.
2007: Kunio Fujiwara; Masashi Shin; David M Hougaard; Lars-Inge Larsson
Distribution of anticancer antibiotic daunomycin in the rat heart and kidney revealed by immunocytochemistry using monoclonal antibodies.
Histochemistry and cell biology 2007;127(1):69-77.
2006: Masashi Shin; Kae Hirokawa; Kunio Fujiwara
Immunoelectron microscopic study of polyamines in the gastrointestinal tract of rat.
Histochemistry and cell biology 2006;125(4):369-75.
2005: Kunio Fujiwara; Hironori Takatsu; Kazuhiro Tsukamoto
Immunocytochemistry for drugs containing an aliphatic primary amino group in the molecule, anticancer antibiotic daunomycin as a model.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2005;53(4):467-74.
2001: Kunio Fujiwara; M Karasuyama; I Murata; T Tanabe; M Yabuuchi; Y Inoue; D Tsuru
Immunoelectron-microscopic demonstration of histamine depletion in the gastric enterochromaffin-like cells of rats treated with alpha-fluoromethylhistidine.
Cell and tissue research 2001;306(2):295-300.
2001: Kunio Fujiwara; T Tanabe; M Yabuuchi; R Ueoka; D Tsuru
A monoclonal antibody against the glutaraldehyde-conjugated polyamine, putrescine: application to immunocytochemistry.
Histochemistry and cell biology 2001;115(6):471-7.
1999: Kunio Fujiwara; I Murata; S Yagisawa; T Tanabe; M Yabuuchi; R Sakakibara; D Tsuru
Glutaraldehyde (GA)-hapten adducts, but without a carrier protein, for use in a specificity study on an antibody against a GA-conjugated hapten compound: histamine monoclonal antibody (AHA-2) as a model.
Journal of biochemistry 1999;126(6):1170-4.
1999: Kunio Fujiwara; Y Masuyama; S Yagisawa; T Tanabe; M Yabuuchi; D Tsuru
Histamine monoclonal antibody for brain immunocytochemistry.
Journal of biochemistry 1999;126(3):503-9.
1999: Kunio Fujiwara; G Bai; C Tamura; D Tsuru
Immunocytochemical localization of histamine in enterochromaffin-like (ECL) cells in rat oxyntic mucosa: a transmission electron microscopy study using monoclonal antibodies and conventional glutaraldehyde-based fixation.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 1999;47(8):1031-8.
1998: Kunio Fujiwara; G Bai; T Kitagawa; D Tsuru
Immunoelectron microscopic study for polyamines.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 1998;46(11):1321-8.
1998: Kunio Fujiwara; Y Kaminishi; Y Inoue; M Yabuuchi
Monoclonal antibody monospecific to glycine for brain immunocytochemistry.
Brain research 1998;806(2):210-8.
1998: Kunio Fujiwara; Y Kaminishi; T Kitagawa; D Tsuru; M Yabuuchi; H Kanetake; K Nomata
Preparation of monoclonal antibodies against N-(gamma-maleimidobutyryloxy)succinimide (GMBS)-conjugated acetylspermine, and development of an enzyme-linked immunosorbent assay (ELISA) for N1,N12-diacetylspermine.
Journal of biochemistry 1998;124(1):244-9.
1998: G Bai; Kunio Fujiwara; Y Kaminishi; H Z Li; T Kitagawa
Enzyme immunoassay for a characteristic protein in the animal crude drug lumbricus.
Biological & pharmaceutical bulletin 1998;21(3):197-201.
1997: G Bai; Kunio Fujiwara; H Tanimori; T Kitagawa
Development and application of a sandwich enzyme immunoassay for Glycyrrhizae Radix protein (GRP) using monoclonal antibodies.
Biological & pharmaceutical bulletin 1997;20(12):1224-8.
1997: Kunio Fujiwara; G Bai; T Kitagawa
Polyamine-like immunoreactivity in rat neurons.
Brain research 1997;767(1):166-71.
1997: T Kitagawa; G Bai; Kunio Fujiwara; A Akahori; S Sonoi; S Kondo; H Z Li
Application of a licorice root specific protein to a general method for the assay of licorice root components in traditional Chinese medicines.
Biological & pharmaceutical bulletin 1997;20(6):589-95.
1997: T Kitagawa; Z Yang; G Bai; Kunio Fujiwara; A Akahori; S Sonoi; S Kondo
A general enzyme immunoassay for the licorice root component contained in traditional Chinese medicines.
Biological & pharmaceutical bulletin 1997;20(3):211-6.
1997: Kunio Fujiwara; T Kitagawa; Y Inoue; G Alonso
Monoclonal antibodies against glutaraldehyde-conjugated histamine: application to immunocytochemistry.
Histochemistry and cell biology 1997;107(1):39-45.
1996: Kunio Fujiwara; Y Masuyama; T Kitagawa
Immunocytochemical localization of polyamines in the gastrointestinal tracts of rats and mice.
Histochemistry and cell biology 1996;106(5):465-71.
1996: T Kitagawa; G Bai; Kunio Fujiwara; A Akahori; S Sonoi; S Kondo
A new method for the detection and quantitative measurement of the contents of trichosanthes root component composing Chinese traditional medicines.
Biological & pharmaceutical bulletin 1996;19(6):783-90.
1996: T Kitagawa; G Bai; Kunio Fujiwara
Specificities of five kinds of antisera produced against crude drugs, pinella tuber, hoelen, glycyrrhizae radix, trichosanthes root and panax ginseng.
Biological & pharmaceutical bulletin 1996;19(3):335-40.
1995: Kunio Fujiwara; H Kanetake; K Furukawa; Y Masuyama; G Bai; H Tanimori; T Kitagawa
Determination of urinary acetylpolyamines by a monoclonal antibody-based enzyme-linked immunosorbent assay (ELISA).
Journal of biochemistry 1995;118(6):1211-5.
1995: Kunio Fujiwara; Y Masuyama
Monoclonal antibody against the glutaraldehyde-conjugated polyamine, spermine.
Histochemistry and cell biology 1995;104(4):309-16.
1994: Kunio Fujiwara; K Furukawa; E Nakayama; H Shiku
Production and characterization of monoclonal antibodies against the polyamine, spermine: immunocytochemical localization in rat tissues.
Histochemistry 1994;102(5):397-404.
1994: Kunio Fujiwara; N Matsumoto; Y Masuyama; T Kitagawa; Y Inoue; K Inouye; D M Hougaard
New hapten-protein conjugation method using N-(m-aminobenzoyloxy) succinimide as a two-level heterobifunctional agent: thyrotropin-releasing hormone as a model peptide without free amino or carboxyl groups.
Journal of immunological methods 1994;175(1):123-9.
1994: Kunio Fujiwara
An evaluation of polyamine immunocytochemistry using immunocytochemical model systems.
Histochemistry 1994;101(4):287-94.
1993: Kunio Fujiwara; T Saita; T Kitagawa
Enzyme-linked immunosorbent assay (ELISA) for luteinizing hormone releasing hormone (LH-RH) using a heterobifunctional cross-linking agent, N-[beta-(4-diazophenyl)ethyl]maleimide.
Biological & pharmaceutical bulletin 1993;16(11):1073-7.
1993: Kunio Fujiwara; T Kitagawa
A new enzyme-linked immunosorbent assay (ELISA) for spermidine using glutaraldehyde coupling of the hapten to carrier-coated microtiter plates.
Journal of biochemistry 1993;114(5):708-13.
1993: Kunio Fujiwara; M Araki; T Kitagawa; Y Inoue
A new enzyme-linked immunosorbent assay (ELISA) for studying immunocytochemical procedures using an antiserum produced against spermidine as a model.
Histochemistry 1993;99(6):477-83.
1990: Kunio Fujiwara; N Matsumoto; T Kitagawa; K Inouye
The use of N-(aminobenzoyloxy) succinimide as a two-level heterobifunctional agent for the preparation of hapten-protein conjugates. Daunomycin as a model hapten with an amino group.
Journal of immunological methods 1990;134(2):227-35.
1988: Kunio Fujiwara; T Saita; N Takenawa; N Matsumoto; T Kitagawa
Enzyme-linked immunosorbent assay for the quantification of actinomycin D using beta-D-galactosidase as a label.
Cancer research 1988;48(17):4843-7.
1988: Kunio Fujiwara; N Matsumoto; S Yagisawa; H Tanimori; T Kitagawa; M Hirota; K Hiratani; K Fukushima; A Tomonaga; K Hara
Sandwich enzyme immunoassay of tumor-associated antigen sialosylated Lewisx using beta-D-galactosidase coupled to a monoclonal antibody of IgM isotype.
Journal of immunological methods 1988;112(1):77-83.
1988: Kunio Fujiwara; T Saita; T Kitagawa
The use of N-[beta-(4-diazophenyl)ethyl]maleimide as a coupling agent in the preparation of enzyme-antibody conjugates.
Journal of immunological methods 1988;110(1):47-53.
1987: Kunio Fujiwara; T Saita; T Shimoda; S Asai; K Hara
Isonicotinic acid hydrazide as an antigen.
The Journal of allergy and clinical immunology 1987;80(4):582-5.