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Eric Geertsma

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Chemicals & Drugs

Physiology

Gene Expression Regulation, Bacterial

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Publications

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Ravi K R Marreddy; Hjalmar P Permentier; Joao P C Pinto; Fabrizia Fusetti; Eric R Geertsma; Jan Kok; Oscar P Kuipers; Justina C Wolters; Bert Poolman
The response of Lactococcus lactis to membrane protein production.
Daniel M Linares; Eric R Geertsma; Bert Poolman
Evolved Lactococcus lactis strains for enhanced expression of recombinant membrane proteins.
Ravi Marreddy; Eric R Geertsma; Hjalmar P Permentier; Joao P C Pinto; Jan Kok; Bert Poolman
Amino acid accumulation limits the overexpression of proteins in Lactococcus lactis.

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All Publications

2011: Ravi K R Marreddy; Hjalmar P Permentier; Joao P C Pinto; Fabrizia Fusetti; Eric R Geertsma; Jan Kok; Oscar P Kuipers; Justina C Wolters; Bert Poolman
The response of Lactococcus lactis to membrane protein production.
PloS one 2011;6(8):e24060.
2010: Daniel M Linares; Eric R Geertsma; Bert Poolman
Evolved Lactococcus lactis strains for enhanced expression of recombinant membrane proteins.
Journal of molecular biology 2010;401(1):45-55.
2010: Ravi Marreddy; Eric R Geertsma; Hjalmar P Permentier; Joao P C Pinto; Jan Kok; Bert Poolman
Amino acid accumulation limits the overexpression of proteins in Lactococcus lactis.
PloS one 2010;5(4):e10317.
2010: Eric R Geertsma; Bert Poolman
Production of membrane proteins in Escherichia coli and Lactococcus lactis.
Methods in molecular biology (Clifton, N.J.) 2010;601():17-38.
2009: Johanna M Kuiper; Radek Pluta; Wim H C Huibers; Fabrizia Fusetti; Eric R Geertsma; Bert Poolman
A method for site-specific labeling of multiple protein thiols.
Protein science : a publication of the Protein Society 2009;18(5):1033-41.
2008: Eric R Geertsma; Maarten Groeneveld; Dirk-Jan Slotboom; Bert Poolman
Quality control of overexpressed membrane proteins.
Proceedings of the National Academy of Sciences of the United States of America 2008;105(15):5722-7.
2008: Eric R Geertsma; N A B Nik Mahmood; Gea K Schuurman-Wolters; Bert Poolman
Membrane reconstitution of ABC transporters and assays of translocator function.
Nature protocols 2008;3(2):256-66.
2007: Eric R Geertsma; Bert Poolman
High-throughput cloning and expression in recalcitrant bacteria.
Nature methods 2007;4(9):705-7.
2007: Ria H Duurkens; Menno B Tol; Eric R Geertsma; Hjalmar P Permentier; Dirk-Jan Slotboom
Flavin binding to the high affinity riboflavin transporter RibU.
The Journal of biological chemistry 2007;282(14):10380-6.
2007: Bert Poolman; Eric R Geertsma; Dirk-Jan Slotboom
Biochemistry. A missing link in membrane protein evolution.
Science (New York, N.Y.) 2007;315(5816):1229-31.
2006: Catherine M Burgess; Dirk-Jan Slotboom; Eric R Geertsma; Ria H Duurkens; Bert Poolman; Douwe van Sinderen
The riboflavin transporter RibU in Lactococcus lactis: molecular characterization of gene expression and the transport mechanism.
Journal of bacteriology 2006;188(8):2752-60.
2005: Eric R Geertsma; Ria H Duurkens; Bert Poolman
The activity of the lactose transporter from Streptococcus thermophilus is increased by phosphorylated IIA and the action of beta-galactosidase.
Biochemistry 2005;44(48):15889-97.
2005: Eric R Geertsma; Ria H Duurkens; Bert Poolman
Functional interactions between the subunits of the lactose transporter from Streptococcus thermophilus.
Journal of molecular biology 2005;350(1):102-11.
2005: Elisa B Vervoort; Jelle B Bultema; Gea K Schuurman-Wolters; Eric R Geertsma; Jaap Broos; Bert Poolman
The first cytoplasmic loop of the mannitol permease from Escherichia coli is accessible for sulfhydryl reagents from the periplasmic side of the membrane.
Journal of molecular biology 2005;346(3):733-43.
2005: Mark K Doeven; Joost H A Folgering; Victor Krasnikov; Eric R Geertsma; Geert van den Bogaart; Bert Poolman
Distribution, lateral mobility and function of membrane proteins incorporated into giant unilamellar vesicles.
Biophysical journal 2005;88(2):1134-42.
2005: Bert Poolman; Mark K Doeven; Eric R Geertsma; Esther Biemans-Oldehinkel; Wil N Konings; Douglas C Rees
Functional analysis of detergent-solubilized and membrane-reconstituted ATP-binding cassette transporters.
Methods in enzymology 2005;400():429-59.
2003: Eric R Geertsma; Ria H Duurkens; Bert Poolman
Identification of the dimer interface of the lactose transport protein from Streptococcus thermophilus.
Journal of molecular biology 2003;332(5):1165-74.
2000: Liesbeth M Veenhoff; Eric R Geertsma; J Knol; Bert Poolman
Close approximation of putative alpha -helices II, IV, VII, X, and XI in the translocation pathway of the lactose transport protein of Streptococcus thermophilus.
The Journal of biological chemistry 2000;275(31):23834-40.