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Gianfranco Gilardi

Research Profile

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Electrochemistry

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Protein Engineering

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Desulfovibrio vulgaris

Physiology

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Publications

TOP 3
Anastasia Sideri; Georgia Eleni Tsotsou; Giovanna Di Nardo; Abhineet Goyal; Gianfranco Gilardi
Hydroxylation of non-substituted polycyclic aromatic hydrocarbons by cytochrome P450 BM3 engineered by directed evolution.
Georgia Eleni Tsotsou; Loretta Lazzarato; Sheila J Sadeghi; Massimo Bertinaria; Giovanna Di Nardo; Roberta Fruttero; Gianfranco Gilardi
A rapid screening for cytochrome P450 catalysis on new chemical entities: cytochrome P450 BM3 and 1,2,5-oxadiazole derivatives.
Sheila J Sadeghi; Gianfranco Gilardi
Chimeric P450 enzymes: Activity of artificial redox fusions driven by different reductases for biotechnological applications.

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All Publications

2013: Anastasia Sideri; Georgia Eleni Tsotsou; Giovanna Di Nardo; Abhineet Goyal; Gianfranco Gilardi
Hydroxylation of non-substituted polycyclic aromatic hydrocarbons by cytochrome P450 BM3 engineered by directed evolution.
Journal of inorganic biochemistry 2013;120():1-7.
2013: Georgia Eleni Tsotsou; Loretta Lazzarato; Sheila J Sadeghi; Massimo Bertinaria; Giovanna Di Nardo; Roberta Fruttero; Gianfranco Gilardi
A rapid screening for cytochrome P450 catalysis on new chemical entities: cytochrome P450 BM3 and 1,2,5-oxadiazole derivatives.
Journal of biomolecular screening 2013;18(2):211-8.
2013: Sheila J Sadeghi; Gianfranco Gilardi
Chimeric P450 enzymes: Activity of artificial redox fusions driven by different reductases for biotechnological applications.
Biotechnology and applied biochemistry 2013;60(1):102-10.
2013: Giovanna Di Nardo; Gianfranco Gilardi
Human aromatase: Perspectives in biochemistry and biotechnology.
Biotechnology and applied biochemistry 2013;60(1):92-101.
2013: Gianluca Catucci; Gianfranco Gilardi; Massimo Maffei; Andrea Occhipinti; Sheila J Sadeghi
Effect of human flavin-containing monooxygenase 3 polymorphism on the metabolism of aurora kinase inhibitors.
International journal of molecular sciences 2013;14(2):2707-16.
2012: Silvia Castrignanò; Sheila J Sadeghi; Gianfranco Gilardi
Entrapment of human flavin-containing monooxygenase 3 in the presence of gold nanoparticles: TEM, FTIR and electrocatalysis.
Biochimica et biophysica acta 2012;1820(12):2072-8.
2012: Francesco Rua; Sheila J Sadeghi; Silvia Castrignanò; Giovanna Di Nardo; Gianfranco Gilardi
Engineering Macaca fascicularis cytochrome P450 2C20 to reduce animal testing for new drugs.
Journal of inorganic biochemistry 2012;117():277-84.
2012: Francesco Rua; Sheila J Sadeghi; Giovanna Di Nardo; Gianfranco Gilardi
Toward reduction in animal sacrifice for drugs: Molecular modeling of Macaca fascicularis P450 2C20 for virtual screening of Homo sapiens P450 2C8 substrates.
Biotechnology and applied biochemistry 2012;59(6):479-89.
2012: Daniela Minerdi; Sheila J Sadeghi; Ivan Zgrablic; Gianfranco Gilardi
Identification of a novel Baeyer-Villiger monooxygenase from Acinetobacter radioresistens: close relationship to the Mycobacterium tuberculosis prodrug activator EtaA.
Microbial biotechnology 2012;5(6):700-16.
2012: Sheila J Sadeghi; Giovanna Di Nardo; Silvia Ferrero; Gianfranco Gilardi
Drug-drug interactions and cooperative effects detected in electrochemically driven human cytochrome P450 3A4.
Bioelectrochemistry (Amsterdam, Netherlands) 2012;86():87-91.
2012: Gianfranco Gilardi; Jian-Jiang Zhong
Editorial.
Biotechnology and applied biochemistry 2012;59(4):269.
2012: Georgia E Tsotsou; Giovanna Di Nardo; Abhineet Goyal; Anastasia Sideri; Gianfranco Gilardi
Identification of mutant Asp251Gly/Gln307His of cytochrome P450 BM3 for the generation of metabolites of diclofenac, ibuprofen and tolbutamide.
Chemistry (Weinheim an der Bergstrasse, Germany) 2012;18(12):3582-8.
2012: Valentina E V Ferrero; Gianluca Catucci; Giovanna Di Nardo; Sheila J Sadeghi; Gianfranco Gilardi
Fluorescence detection of ligand binding to labeled cytochrome P450 BM3.
Dalton transactions (Cambridge, England : 2003) 2012;41(7):2018-25.
2012: Gianluca Catucci; Gianfranco Gilardi; Lars Jeuken; Sheila J Sadeghi
In vitro drug metabolism by C-terminally truncated human flavin-containing monooxygenase 3.
Biochemical pharmacology 2012;83(4):551-8.
2012: Giovanna Di Nardo; Gianfranco Gilardi
Optimization of the Bacterial Cytochrome P450 BM3 System for the Production of Human Drug Metabolites.
International journal of molecular sciences 2012;13(12):15901-24.
2012: Simone Morra; Giovanna Di Nardo; Gianfranco Gilardi; Alberto Giraudo; Paul W King; Francesca Valetti
Site saturation mutagenesis demonstrates a central role for cysteine 298 as proton donor to the catalytic site in CaHydA [FeFe]-hydrogenase.
PloS one 2012;7(10):e48400.
2011: Simone Morra; Sheila J Sadeghi; Francesca Valetti; Paul W King; Toby Meyer; Gianfranco Gilardi
Direct electrochemistry of an [FeFe]-hydrogenase on a TiO2 electrode.
Chemical communications (Cambridge, England) 2011;47(38):10566-8.
2011: Sara Maurelli; Valentina E V Ferrero; Mario Chiesa; Giovanna Di Nardo; Elio Giamello; Gianfranco Gilardi; Sabine Van Doorslaer
Direct spectroscopic evidence for binding of anastrozole to the iron heme of human aromatase. Peering into the mechanism of aromatase inhibition.
Chemical communications (Cambridge, England) 2011;47(38):10737-9.
2011: Andrea Fantuzzi; Lok Hang Mak; Ennio Capria; Vikash Dodhia; Paola Panicco; Stephen Collins; Gianfranco Gilardi
A new standardized electrochemical array for drug metabolic profiling with human cytochromes P450.
Analytical chemistry 2011;83(10):3831-9.
2011: Paola Panicco; Vikash R Dodhia; Andrea Fantuzzi; Gianfranco Gilardi
Enzyme-based amperometric platform to determine the polymorphic response in drug metabolism by cytochromes P450.
Analytical chemistry 2011;83(6):2179-86.
2011: Danilo Degregorio; Sheila J Sadeghi; Giovanna Di Nardo; Gianfranco Gilardi; Sandro P Solinas
Understanding uncoupling in the multiredox centre P450 3A4-BMR model system.
Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry 2011;16(1):109-16.
2011: Sheila J Sadeghi; Andrea Fantuzzi; Gianfranco Gilardi
Breakthrough in P450 bioelectrochemistry and future perspectives.
Biochimica et biophysica acta 2011;1814(1):237-48.
2010: Andrea Fantuzzi; Ennio Capria; Lok Hang Mak; Vikash R Dodhia; Sheila J Sadeghi; Stephen Collins; Graham Somers; Ejaz Huq; Gianfranco Gilardi
An electrochemical microfluidic platform for human P450 drug metabolism profiling.
Analytical chemistry 2010;82(24):10222-7.
2010: Silvia Castrignanò; Sheila J Sadeghi; Gianfranco Gilardi
Electro-catalysis by immobilised human flavin-containing monooxygenase isoform 3 (hFMO3).
Analytical and bioanalytical chemistry 2010;398(3):1403-9.
2010: Lok Hang Mak; Sheila J Sadeghi; Andrea Fantuzzi; Gianfranco Gilardi
Control of human cytochrome P450 2E1 electrocatalytic response as a result of unique orientation on gold electrodes.
Analytical chemistry 2010;82(12):5357-62.
2010: Sheila J Sadeghi; Rita Meirinhos; Gianluca Catucci; Vikash R Dodhia; Giovanna Di Nardo; Gianfranco Gilardi
Direct electrochemistry of drug metabolizing human flavin-containing monooxygenase: electrochemical turnover of benzydamine and tamoxifen.
Journal of the American Chemical Society 2010;132(2):458-9.
2009: Giovanna Di Nardo; Carlo Roggero; Simona Campolongo; Francesca Valetti; Francesco Trotta; Gianfranco Gilardi
Catalytic properties of catechol 1,2-dioxygenase from Acinetobacter radioresistens S13 immobilized on nanosponges.
Dalton transactions (Cambridge, England : 2003) 2009;(33):6507-12.
2009: Giovanna Di Nardo; Almerinda Di Venere; Giampiero Mei; Sheila J Sadeghi; Jon R Wilson; Gianfranco Gilardi
Engineering heme binding sites in monomeric rop.
Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry 2009;14(4):497-505.
2009: Andrea Fantuzzi; Yergalem T Meharenna; Paul B Briscoe; Francoise Guerlesquin; Sheila J Sadeghi; Gianfranco Gilardi
Characterisation of the electron transfer and complex formation between flavodoxin from D. vulgaris and the haem domain of cytochrome P450 BM3 from B. megaterium.
Biochimica et biophysica acta 2009;1787(4):234-41.
2009: Andrea Fantuzzi; Roberto Artali; Gabriella Bombieri; Nicoletta Marchini; Fiorella Meneghetti; Gianfranco Gilardi; Sheila J Sadeghi; Davide Cavazzini; Gian Luigi Rossi
Redox properties and crystal structures of a Desulfovibrio vulgaris flavodoxin mutant in the monomeric and homodimeric forms.
Biochimica et biophysica acta 2009;1794(3):496-505.
2008: Valentina E V Ferrero; Laura Andolfi; Giovanna Di Nardo; Sheila J Sadeghi; Andrea Fantuzzi; Salvatore Cannistraro; Gianfranco Gilardi
Protein and electrode engineering for the covalent immobilization of P450 BMP on gold.
Analytical chemistry 2008;80(22):8438-46.
2008: Paola Panicco; Yeni Astuti; Andrea Fantuzzi; James R Durrant; Gianfranco Gilardi
P450 versus P420: correlation between cyclic voltammetry and visible absorption spectroscopy of the immobilized heme domain of cytochrome P450 BM3.
The journal of physical chemistry. B 2008;112(44):14063-8.
2007: Giovanna Di Nardo; Andrea Fantuzzi; Anastasia Sideri; Paola Panicco; Carlo Sassone; Carlo Giunta; Gianfranco Gilardi
Wild-type CYP102A1 as a biocatalyst: turnover of drugs usually metabolised by human liver enzymes.
Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry 2007;12(3):313-23.
2006: Vikash Rajnikant Dodhia; Andrea Fantuzzi; Gianfranco Gilardi
Engineering human cytochrome P450 enzymes into catalytically self-sufficient chimeras using molecular Lego.
Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry 2006;11(7):903-16.
2006: Andrea Fantuzzi; Yergalem T Meharenna; Paul B Briscoe; Carlo Sassone; Beatrice Borgia; Gianfranco Gilardi
Improving catalytic properties of P450 BM3 haem domain electrodes by molecular Lego.
Chemical communications (Cambridge, England) 2006;(12):1289-91.
2005: Michael Fairhead; Silva Giannini; Elizabeth M J Gillam; Gianfranco Gilardi
Functional characterisation of an engineered multidomain human P450 2E1 by molecular Lego.
Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry 2005;10(8):842-53.
2004: Francesca Valetti; Gianfranco Gilardi
Directed evolution of enzymes for product chemistry.
Natural product reports 2004;21(4):490-511.
2004: Yeni Astuti; Emmanuel Topoglidis; Paul B Briscoe; Andrea Fantuzzi; Gianfranco Gilardi; James R Durrant
Proton-coupled electron transfer of flavodoxin immobilized on nanostructured tin dioxide electrodes: thermodynamics versus kinetics control of protein redox function.
Journal of the American Chemical Society 2004;126(25):8001-9.
2004: Yeni Astuti; Emmanuel Topoglidis; Gianfranco Gilardi; James R Durrant
Cyclic voltammetry and voltabsorptometry studies of redox proteins immobilised on nanocrystalline tin dioxide electrodes.
Bioelectrochemistry (Amsterdam, Netherlands) 2004;63(1-2):55-9.
2004: Andrea Fantuzzi; Michael Fairhead; Gianfranco Gilardi
Direct electrochemistry of immobilized human cytochrome P450 2E1.
Journal of the American Chemical Society 2004;126(16):5040-1.
2003: Sara Divari; Francesca Valetti; Patrizia Caposio; Enrica Pessione; Maria Cavaletto; Ersilia Griva; Giorgio Gribaudo; Gianfranco Gilardi; Carlo Giunta
The oxygenase component of phenol hydroxylase from Acinetobacter radioresistens S13.
European journal of biochemistry / FEBS 2003;270(10):2244-53.
2003: Jon R Wilson; Daren J Caruana; Gianfranco Gilardi
Engineering redox functions in a nucleic acid binding protein.
Chemical communications (Cambridge, England) 2003;(3):356-7.
2002: Roberto Artali; Gabriella Bombieri; Fiorella Meneghetti; Gianfranco Gilardi; Sheila J Sadeghi; Davide Cavazzini; Gian Luigi Rossi
Comparison of the refined crystal structures of wild-type (1.34 A) flavodoxin from Desulfovibrio vulgaris and the S35C mutant (1.44 A) at 100 K.
Acta crystallographica. Section D, Biological crystallography 2002;58(Pt 10 Pt 2):1787-92.
2002: Andrea Fantuzzi; Sheila J Sadeghi; Francesca Valetti; Gian Luigi Rossi; Gianfranco Gilardi
Tuning the reduction potential of engineered cytochrome c-553.
Biochemistry 2002;41(27):8718-24.
2002: Tony Cass; Jim Dunwell; Lawrence P Wackett; Gianfranco Gilardi; Thomas A Kost; Patrick Condreay; Steven Projan; Jeroen Hugenholtz; Michiel Kleerebezem; Nicholas J Turner; Robert E Speight
Biotechnology. Paper Alert.
Current opinion in biotechnology 2002;13(1):1-8.
2002: Gianfranco Gilardi; Yergalem T Meharenna; Georgia Eleni Tsotsou; Sheila J Sadeghi; Michael Fairhead; Silva Giannini
Molecular Lego: design of molecular assemblies of P450 enzymes for nanobiotechnology.
Biosensors & bioelectronics 2002;17(1-2):133-45.
2002: Georgia Eleni Tsotsou; Anthony E G Cass; Gianfranco Gilardi
High throughput assay for cytochrome P450 BM3 for screening libraries of substrates and combinatorial mutants.
Biosensors & bioelectronics 2002;17(1-2):119-31.
2001: Gianfranco Gilardi; Andrea Fantuzzi
Manipulating redox systems: application to nanotechnology.
Trends in biotechnology 2001;19(11):468-76.
2001: Gianfranco Gilardi; Andrea Fantuzzi; Sheila J Sadeghi
Engineering and design in the bioelectrochemistry of metalloproteins.
Current opinion in structural biology 2001;11(4):491-9.
2000: Sheila J Sadeghi; Francesca Valetti; C A Cunha; Maria João Romão; C M Soares; Gianfranco Gilardi
Ionic strength dependence of the non-physiological electron transfer between flavodoxin and cytochrome c553 from D vulgaris.
Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry 2000;5(6):730-7.
2000: Sheila J Sadeghi; Yergalem T Meharenna; Andrea Fantuzzi; Francesca Valetti; Gianfranco Gilardi
Engineering artificial redox chains by molecular 'Lego'.
Faraday discussions 2000;(116):135-53; discussion 171-90.
1999: Giampiero Mei; Almerinda Di Venere; F M Campeggi; Gianfranco Gilardi; Nicola Rosato; Fabio De Matteis; Alessandro Finazzi-Agrò
The effect of pressure and guanidine hydrochloride on azurins mutated in the hydrophobic core.
European journal of biochemistry / FEBS 1999;265(2):619-26.
1999: Enrica Pessione; Sara Divari; Ersilia Griva; Maria Cavaletto; Gian Luigi Rossi; Gianfranco Gilardi; Carlo Giunta
Phenol hydroxylase from Acinetobacter radioresistens is a multicomponent enzyme. Purification and characterization of the reductase moiety.
European journal of biochemistry / FEBS 1999;265(2):549-55.
1999: C A Cunha; Maria João Romão; Sheila J Sadeghi; Francesca Valetti; Gianfranco Gilardi; C M Soares
Effects of protein-protein interactions on electron transfer: docking and electron transfer calculations for complexes between flavodoxin and c-type cytochromes.
Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry 1999;4(3):360-74.
1998: Emmanuel Topoglidis; N Beaumont; Anthony E G Cass; Gianfranco Gilardi; Sheila J Sadeghi; James R Durrant
Protein Adsorption on Nanocrystalline TiO(2) Films: An Immobilization Strategy for Bioanalytical Devices.
Analytical chemistry 1998;70(23):5111-3.
1998: Almerinda Di Venere; Giampiero Mei; Gianfranco Gilardi; Nicola Rosato; Fabio De Matteis; R McKay; E Gratton; Alessandro Finazzi-Agrò
Resolution of the heterogeneous fluorescence in multi-tryptophan proteins: ascorbate oxidase.
European journal of biochemistry / FEBS 1998;257(2):337-43.
1998: Francesca Valetti; Sheila J Sadeghi; Yergalem T Meharenna; S R Leliveld; Gianfranco Gilardi
Engineering multi-domain redox proteins containing flavodoxin as bio-transformer: preparatory studies by rational design.
Biosensors & bioelectronics 1998;13(6):675-85.
1997: Sheila J Sadeghi; Gianfranco Gilardi; Anthony E G Cass
Mediated electrochemistry of peroxidases--effects of variations in protein and mediator structures.
Biosensors & bioelectronics 1997;12(12):1191-8.
1997: Gianfranco Gilardi; Giampiero Mei; Nicola Rosato; Alessandro Finazzi Agrò; Anthony E G Cass
Spectroscopic properties of an engineered maltose binding protein.
Protein engineering 1997;10(5):479-86.
1996: Giampiero Mei; Gianfranco Gilardi; M Venanzi; Nicola Rosato; Gerard W Canters; A F Agró
Probing the structure and mobility of Pseudomonas aeruginosa azurin by circular dichroism and dynamic fluorescence anisotropy.
Protein science : a publication of the Protein Society 1996;5(11):2248-54.
1994: Gianfranco Gilardi; L Q Zhou; L Hibbert; Anthony E G Cass
Engineering the maltose binding protein for reagentless fluorescence sensing.
Analytical chemistry 1994;66(21):3840-7.
1994: Gianfranco Gilardi; Giampiero Mei; Nicola Rosato; Gerard W Canters; Alessandro Finazzi-Agrò
Unique environment of Trp48 in Pseudomonas aeruginosa azurin as probed by site-directed mutagenesis and dynamic fluorescence spectroscopy.
Biochemistry 1994;33(6):1425-32.
1993: Gerard W Canters; Gianfranco Gilardi
Engineering type 1 copper sites in proteins.
FEBS letters 1993;325(1-2):39-48.
1990: Gianfranco Gilardi; P J Harvey; Anthony E G Cass; J M Palmer
Radical intermediates in veratryl alcohol oxidation by ligninase. NMR evidence.
Biochimica et biophysica acta 1990;1041(2):129-32.