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Robert Glaeser

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Image Processing, Computer-Assisted

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TOP 3
Ting Wang; Yong Duan; Robert M Glaeser; Christopher S Lunde; Shahab Rouhani; Ayla O Sessions; Marc T Facciotti
Deprotonation of D96 in bacteriorhodopsin opens the proton uptake pathway.
Bong-Gyoon Han; Peter Hwu; Amos Song; Milton T Stubbs; Ross W Walton; Steven M Yannone; Pablo Arbeláez; Ming Dong; Robert M Glaeser
Electron microscopy of biotinylated protein complexes bound to streptavidin monolayer crystals.
Robert M Glaeser
Electron microscopy of biological specimens in liquid water.

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All Publications

2013: Ting Wang; Yong Duan; Robert M Glaeser; Christopher S Lunde; Shahab Rouhani; Ayla O Sessions; Marc T Facciotti
Deprotonation of D96 in bacteriorhodopsin opens the proton uptake pathway.
Structure (London, England : 1993) 2013;21(2):290-7.
2012: Bong-Gyoon Han; Peter Hwu; Amos Song; Milton T Stubbs; Ross W Walton; Steven M Yannone; Pablo Arbeláez; Ming Dong; Robert M Glaeser
Electron microscopy of biotinylated protein complexes bound to streptavidin monolayer crystals.
Journal of structural biology 2012;180(1):249-53.
2012: Robert M Glaeser
Electron microscopy of biological specimens in liquid water.
Biophysical journal 2012;103(1):163-4; author reply 165-6.
2011: Pablo Arbeláez; Robert M Glaeser; Bong-Gyoon Han; Joseph Lim; Dieter Typke; Jitendra Malik
Experimental evaluation of support vector machine-based and correlation-based approaches to automatic particle selection.
Journal of structural biology 2011;175(3):319-28.
2011: Richard J Hall; Eva Nogales; Robert M Glaeser
Accurate modeling of single-particle cryo-EM images quantitates the benefits expected from using Zernike phase contrast.
Journal of structural biology 2011;174(3):468-75.
2011: Robert M Glaeser; Richard J Hall
Reaching the information limit in cryo-EM of biological macromolecules: experimental aspects.
Biophysical journal 2011;100(10):2331-7.
2011: David M Larson; Kenneth H Downing; Robert M Glaeser
The surface of evaporated carbon films is an insulating, high-bandgap material.
Journal of structural biology 2011;174(2):420-3.
2011: Robert M Glaeser; Dieter Typke; Peter C Tiemeijer; James Pulokas; Anchi Cheng
Precise beam-tilt alignment and collimation are required to minimize the phase error associated with coma in high-resolution cryo-EM.
Journal of structural biology 2011;174(1):1-10.
2011: Robert M Glaeser; G McMullan; A R Faruqi; R Henderson
Images of paraffin monolayer crystals with perfect contrast: minimization of beam-induced specimen motion.
Ultramicroscopy 2011;111(2):90-100.
2009: Bong-Gyoon Han; Ming Dong; Haichuan Liu; Lauren Camp; Jil Geller; Mary Singer; Terry C Hazen; Megan Choi; H Ewa Witkowska; David A Ball; Dieter Typke; Kenneth H Downing; Maxim Shatsky; Steven E Brenner; John-Marc Chandonia; Mark D Biggin; Robert M Glaeser
Survey of large protein complexes in D. vulgaris reveals great structural diversity.
Proceedings of the National Academy of Sciences of the United States of America 2009;106(39):16580-5.
2008: Robert M Glaeser
Retrospective: radiation damage and its associated "information limitations".
Journal of structural biology 2008;163(3):271-6.
2008: Kenneth H Downing; Robert M Glaeser
Restoration of weak phase-contrast images recorded with a high degree of defocus: the "twin image" problem associated with CTF correction.
Ultramicroscopy 2008;108(9):921-8.
2008: Robert M Glaeser
Macromolecular structures without crystals.
Proceedings of the National Academy of Sciences of the United States of America 2008;105(6):1779-80.
2007: Florian Garczarek; Ming Dong; Dieter Typke; Halina Ewa Witkowska; Terry C Hazen; Eva Nogales; Mark D Biggin; Robert M Glaeser
Octomeric pyruvate-ferredoxin oxidoreductase from Desulfovibrio vulgaris.
Journal of structural biology 2007;159(1):9-18.
2007: Rossana Cambie; Kenneth H Downing; Dieter Typke; Robert M Glaeser; Jian Jin
Design of a microfabricated, two-electrode phase-contrast element suitable for electron microscopy.
Ultramicroscopy 2007;107(4-5):329-39.
2007: Dieter Typke; Christopher J Gilpin; Kenneth H Downing; Robert M Glaeser
Stroboscopic image capture: reducing the dose per frame by a factor of 30 does not prevent beam-induced specimen movement in paraffin.
Ultramicroscopy 2007;107(2-3):106-15.
2007: Michael Hohn; Grant Tang; Grant Goodyear; Philip R Baldwin; Zhong Huang; Pawel A Penczek; Chao Yang; Robert M Glaeser; Paul D Adams; Steven J Ludtke
SPARX, a new environment for Cryo-EM image processing.
Journal of structural biology 2007;157(1):47-55.
2007: Chao Yang; Pawel A Penczek; ArDean Leith; Francisco J Asturias; Esmond G Ng; Robert M Glaeser; Joachim Frank
The parallelization of SPIDER on distributed-memory computers using MPI.
Journal of structural biology 2007;157(1):240-9.
2006: Christopher S Lunde; Shahab Rouhani; Marc T Facciotti; Robert M Glaeser
Membrane-protein stability in a phospholipid-based crystallization medium.
Journal of structural biology 2006;154(3):223-31.
2005: Umesh Adiga; William T Baxter; Richard J Hall; Beate Rockel; Bimal K Rath; Joachim Frank; Robert M Glaeser
Particle picking by segmentation: a comparative study with SPIDER-based manual particle picking.
Journal of structural biology 2005;152(3):211-20.
2005: Beate Rockel; Jürgen Peters; Shirley A Müller; Gönül Seyit; Philippe Ringler; Reiner Hegerl; Robert M Glaeser; Wolfgang Baumeister
Molecular architecture and assembly mechanism of Drosophila tripeptidyl peptidase II.
Proceedings of the National Academy of Sciences of the United States of America 2005;102(29):10135-40.
2005: Dieter Typke; Robert A Nordmeyer; Arthur Jones; Juyoung Lee; Agustin Avila-Sakar; Kenneth H Downing; Robert M Glaeser
High-throughput film-densitometry: an efficient approach to generate large data sets.
Journal of structural biology 2005;149(1):17-29.
2004: Robert M Glaeser; Kenneth H Downing
Specimen charging on thin films with one conducting layer: discussion of physical principles.
Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada 2004;10(6):790-6.
2004: Kenneth H Downing; M R McCartney; Robert M Glaeser
Experimental characterization and mitigation of specimen charging on thin films with one conducting layer.
Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada 2004;10(6):783-9.
2004: Marc T Facciotti; Shahab Rouhani-Manshadi; Robert M Glaeser
Energy transduction in transmembrane ion pumps.
Trends in biochemical sciences 2004;29(8):445-51.
2004: Marc T Facciotti; Vincent S Cheung; Christopher S Lunde; Shahab Rouhani; Nitin S Baliga; Robert M Glaeser
Specificity of anion binding in the substrate pocket of bacteriorhodopsin.
Biochemistry 2004;43(17):4934-43.
2004: Marc T Facciotti; Shahab Rouhani; Robert M Glaeser
Crystal structures of bR(D85S) favor a model of bacteriorhodopsin as a hydroxyl-ion pump.
FEBS letters 2004;564(3):301-6.
2004: Dieter Typke; Kenneth H Downing; Robert M Glaeser
Electron microscopy of biological macromolecules: bridging the gap between what physics allows and what we currently can get.
Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada 2004;10(1):21-7.
2004: P S Umesh Adiga; Ravi Malladi; William Baxter; Robert M Glaeser
A binary segmentation approach for boxing ribosome particles in cryo EM micrographs.
Journal of structural biology 2004;145(1-2):142-51.
2004: Robert M Glaeser
Historical background: Why is it important to improve automated particle selection methods?
Journal of structural biology 2004;145(1-2):15-8.
2004: Yuanxin Zhu; Bridget Carragher; Robert M Glaeser; Denis Fellmann; Chandrajit Bajaj; Marshall Bern; Fabrice Mouche; Felix de Haas; Richard J Hall; David J Kriegman; Steven J Ludtke; Satya P Mallick; Pawel A Penczek; Alan M Roseman; Fred J Sigworth; Niels Volkmann; Clinton Potter
Automatic particle selection: results of a comparative study.
Journal of structural biology 2004;145(1-2):3-14.
2003: J C H Spence; Uwe Weierstall; T T Fricke; Robert M Glaeser; Kenneth H Downing
Three-dimensional diffractive imaging for crystalline monolayers with one-dimensional compact support.
Journal of structural biology 2003;144(1-2):209-18.
2003: Marc T Facciotti; Vincent S Cheung; Doris Nguyen; Shahab Rouhani; Robert M Glaeser
Crystal structure of the bromide-bound D85S mutant of bacteriorhodopsin: principles of ion pumping.
Biophysical journal 2003;85(1):451-8.
2002: Beate Rockel; Jürgen Peters; Brigitte Kühlmorgen; Robert M Glaeser; Wolfgang Baumeister
A giant protease with a twist: the TPP II complex from Drosophila studied by electron microscopy.
The EMBO journal 2002;21(22):5979-84.
2002: Shijun Zhong; Voichita M Dadarlat; Robert M Glaeser; Teresa Head-Gordon; Kenneth H Downing
Modeling chemical bonding effects for protein electron crystallography: the transferable fragmental electrostatic potential (TFESP) method.
Acta crystallographica. Section A, Foundations of crystallography 2002;58(Pt 2):162-70.
2002: Shahab Rouhani; Marc T Facciotti; Glenna Woodcock; Vincent S Cheung; Christian Cunningham; Doris Nguyen; Behzad Rad; Chiann-Tso Lin; Christopher S Lunde; Robert M Glaeser
Crystallization of membrane proteins from media composed of connected-bilayer gels.
Biopolymers 2002;66(5):300-16.
2001: Marc T Facciotti; Shahab Rouhani; FT Burkard; F M Betancourt; K H Downing; R B Rose; G McDermott; Robert M Glaeser
Structure of an early intermediate in the M-state phase of the bacteriorhodopsin photocycle.
Biophysical journal 2001;81(6):3442-55.
2001: Shahab Rouhani; Jean-Philippe Cartailler; Marc T Facciotti; Peter J Walian; Richard Needleman; Janos K Lanyi; Robert M Glaeser; Hartmut Luecke
Crystal structure of the D85S mutant of bacteriorhodopsin: model of an O-like photocycle intermediate.
Journal of molecular biology 2001;313(3):615-28.
2001: William V Nicholson; Robert M Glaeser
Review: automatic particle detection in electron microscopy.
Journal of structural biology 2001;133(2-3):90-101.
2000: F M Betancourt; Robert M Glaeser
Chemical and physical evidence for multiple functional steps comprising the M state of the bacteriorhodopsin photocycle.
Biochimica et biophysica acta 2000;1460(1):106-18.
2000: Robert M Glaeser; Marc T Facciotti; Peter J Walian; Shahab Rouhani; J Holton; Alastair A MacDowell; Richard S Celestre; Daniella Cambie; Howard A Padmore
Characterization of conditions required for X-Ray diffraction experiments with protein microcrystals.
Biophysical journal 2000;78(6):3178-85.
1999: Robert M Glaeser
Review: electron crystallography: present excitement, a nod to the past, anticipating the future.
Journal of structural biology 1999;128(1):3-14.
1999: S Chang; Teresa Head-Gordon; Robert M Glaeser; Kenneth H Downing
Chemical bonding effects in the determination of protein structures by electron crystallography.
Acta crystallographica. Section A, Foundations of crystallography 1999;55 ( Pt 2 Pt 2)():305-13.
1998: F M Hendrickson; FT Burkard; Robert M Glaeser
Structural characterization of the L-to-M transition of the bacteriorhodopsin photocycle.
Biophysical journal 1998;75(3):1446-54.
1997: Teresa Head-Gordon; Jon M Sorenson; Alexander Pertsemlidis; Robert M Glaeser
Differences in hydration structure near hydrophobic and hydrophilic amino acids.
Biophysical journal 1997;73(4):2106-15.
1996: Alexander Pertsemlidis; A M Saxena; A K Soper; Teresa Head-Gordon; Robert M Glaeser
Direct evidence for modified solvent structure within the hydration shell of a hydrophobic amino acid.
Proceedings of the National Academy of Sciences of the United States of America 1996;93(20):10769-74.
1995: B F McEwen; Kenneth H Downing; Robert M Glaeser
The relevance of dose-fractionation in tomography of radiation-sensitive specimens.
Ultramicroscopy 1995;60(3):357-73.
1995: Guy Perkins; Kenneth H Downing; Robert M Glaeser
Crystallographic extraction and averaging of data from small image areas.
Ultramicroscopy 1995;60(2):283-94.
1994: B G Han; Janet Vonck; Robert M Glaeser
The bacteriorhodopsin photocycle: direct structural study of two substrates of the M-intermediate.
Biophysical journal 1994;67(3):1179-86.
1994: Janet Vonck; B G Han; F Burkard; Guy Perkins; Robert M Glaeser
Two progressive substrates of the M-intermediate can be identified in glucose-embedded, wild-type bacteriorhodopsin.
Biophysical journal 1994;67(3):1173-8.
1994: B G Han; Sharon Wolf; Janet Vonck; Robert M Glaeser
Specimen flatness of glucose-embedded biological materials for electron crystallography is affected significantly by the choice of carbon evaporation stock.
Ultramicroscopy 1994;55(1):1-5.
1994: Robert M Glaeser
Probing toward atomic resolution in molecular topography.
Proceedings of the National Academy of Sciences of the United States of America 1994;91(6):1981-2.
1994: Robert M Glaeser
Overview of significant challenges in molecular biology amenable to computational methods.
Basic life sciences 1994;63():11-8; discussion 18-9.
1993: Robert M Glaeser; Kenneth H Downing
High-resolution electron crystallography of protein molecules.
Ultramicroscopy 1993;52(3-4):478-86.
1993: Guy Perkins; F Burkard; E Liu; Robert M Glaeser
Glucose alone does not completely hydrate bacteriorhodopsin in glucose-embedded purple membrane.
Journal of microscopy 1993;169(Pt 1):61-5.
1992: Robert M Glaeser; Kenneth H Downing
Assessment of resolution in biological electron crystallography.
Ultramicroscopy 1992;47(1-3):256-65.
1992: Robert M Glaeser
Specimen flatness of thin crystalline arrays: influence of the substrate.
Ultramicroscopy 1992;46(1-4):33-43.
1992: Guy Perkins; E Liu; F Burkard; E A Berry; Robert M Glaeser
Characterization of the conformational change in the M1 and M2 substates of bacteriorhodopsin by the combined use of visible and infrared spectroscopy.
Journal of structural biology 1992;109(2):142-51.
1991: S S Sikerwar; Kenneth H Downing; Robert M Glaeser
Three-dimensional structure of an invertebrate intercellular communicating junction.
Journal of structural biology 1991;106(3):255-63.
1991: Robert M Glaeser; Kenneth H Downing; Bing K Jap
What spectroscopy can still tell us about the secondary structure of bacteriorhodopsin.
Biophysical journal 1991;59(4):934-8.
1991: Robert M Glaeser; A Zilker; M Radermacher; H E Gaub; T Hartmann; W Baumeister
Interfacial energies and surface-tension forces involved in the preparation of thin, flat crystals of biological macromolecules for high-resolution electron microscopy.
Journal of microscopy 1991;161(Pt 1):21-45.
1989: Robert M Glaeser; T A Ceska
High-voltage electron diffraction from bacteriorhodopsin (purple membrane) is measurably dynamical.
Acta crystallographica. Section A, Foundations of crystallography 1989;45 ( Pt 9)():620-8.
1989: Robert M Glaeser; L Tong; S H Kim
Three-dimensional reconstructions from incomplete data: interpretability of density maps at "atomic" resolution.
Ultramicroscopy 1989;27(3):307-18.
1988: M H Ho; Bing K Jap; Robert M Glaeser
Validity domain of the weak-phase-object approximation for electron diffraction of thin protein crystals.
Acta crystallographica. Section A, Foundations of crystallography 1988;44 ( Pt 6)():878-84.
1987: J S Jaffe; Robert M Glaeser
Difference Fourier analysis of "surface features" of bacteriorhodopsin using glucose-embedded and frozen-hydrated purple membrane.
Ultramicroscopy 1987;23(1):17-28.
1986: Robert M Glaeser; J Baldwin; T A Ceska; R Henderson
Electron diffraction analysis of the M412 intermediate of bacteriorhodopsin.
Biophysical journal 1986;50(5):913-20.
1986: M R Dickson; Kenneth H Downing; W H Wu; Robert M Glaeser
Three-dimensional structure of the surface layer protein of Aquaspirillum serpens VHA determined by electron crystallography.
Journal of bacteriology 1986;167(3):1025-34.
1986: Kenneth H Downing; Robert M Glaeser
Improvement in high resolution image quality of radiation-sensitive specimens achieved with reduced spot size of the electron beam.
Ultramicroscopy 1986;20(3):269-78.
1986: G Sosinsky; R Schekman; Robert M Glaeser
Morphological observations on the formation and stability of the crystalline arrays in the plasma membrane of Saccharomyces cerevisiae.
Journal of ultrastructure and molecular structure research 1986;94(1):37-51.
1985: Robert M Glaeser; Bing K Jap
Absorption flattening in the circular dichroism spectra of small membrane fragments.
Biochemistry 1985;24(23):6398-401.
1985: Robert M Glaeser; J S Jubb; R Henderson
Structural comparison of native and deoxycholate-treated purple membrane.
Biophysical journal 1985;48(5):775-80.
1985: C F Chang; S Mizushima; Robert M Glaeser
Projected structure of the pore-forming OmpC protein from Escherichia coli outer membrane.
Biophysical journal 1985;47(5):629-39.
1984: J S Jaffe; Robert M Glaeser
Preparation of frozen-hydrated specimens for high resolution electron microscopy.
Ultramicroscopy 1984;13(4):373-7.
1983: Bing K Jap; M F Maestre; S B Hayward; Robert M Glaeser
Peptide-chain secondary structure of bacteriorhodopsin.
Biophysical journal 1983;43(1):81-9.
1983: C F Chang; R C Williams; D A Grano; Kenneth H Downing; Robert M Glaeser
Quantitative evaluation of high-resolution features in images of negatively stained Tobacco Mosaic Virus.
Ultramicroscopy 1983;11(1):3-11.
1981: J Smit; D A Grano; Robert M Glaeser; N Agabian
Periodic surface array in Caulobacter crescentus: fine structure and chemical analysis.
Journal of bacteriology 1981;146(3):1135-50.
1980: Kenneth H Downing; Robert M Glaeser
Electron diffraction from single crystals of DNA.
Biophysical journal 1980;32(2):851-6.
1980: S B Hayward; Robert M Glaeser
High resolution cold stage for the JEOL 100B and 100C electron microscopes.
Ultramicroscopy 1980;5(1):3-8.