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Stephen Halford

This is a preview profile on BiomedExperts - the first literature-based scientific social network. It brings the right researchers together and allows them to collaborate online. Collexis and Dell provide the BiomedExperts network of +1.5 Million pre-calculated profiles free of charge to researchers worldwide.

Research Profile (preview)

Chemicals & Drugs

Concepts & Ideas

Living Beings

Escherichia coli

Physiology

Genetic Recombination

Phenomena

Protein Binding

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Co-Publications
Name
12
Szczelkun, Mark
9
Baldwin, GS
8
Bellamy, Stuart
7
Oram, M
7
Gowers, Darren
6
Milsom, Susan
6
Gormley, Niall
5
Daniels, Lucy
5
Embleton, Michelle
5
Williams, Shelley
4
Bath, Abigail
4
Catto, Lucy
4
Sessions, Richard
3
Stanford, NP
3
Marko, John

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Publications

TOP 3
Bellamy Stuart R W; Kovacheva Yana S; Zulkipli Ishan Haji; Halford Stephen E
Differences between Ca2+ and Mg2+ in DNA binding and release by the SfiI restriction endonuclease: implications for DNA looping.
Laurens Niels; Bellamy Stuart R W; Harms August F; Kovacheva Yana S; Halford Stephen E; Wuite Gijs J L
Dissecting protein-induced DNA looping dynamics in real time.
Halford Stephen E
An end to 40 years of mistakes in DNA-protein association kinetics?

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All Publications

2009: Bellamy Stuart R W; Kovacheva Yana S; Zulkipli Ishan Haji; Halford Stephen E
Differences between Ca2+ and Mg2+ in DNA binding and release by the SfiI restriction endonuclease: implications for DNA looping.
Nucleic acids research 2009;37(16):5443-53.
2009: Laurens Niels; Bellamy Stuart R W; Harms August F; Kovacheva Yana S; Halford Stephen E; Wuite Gijs J L
Dissecting protein-induced DNA looping dynamics in real time.
Nucleic acids research 2009;37(16):5454-64.
2009: Halford Stephen E
An end to 40 years of mistakes in DNA-protein association kinetics?
Biochemical Society transactions 2009;37(Pt 2):343-8.
2009: Sanders Kelly L; Catto Lucy E; Bellamy Stuart R W; Halford Stephen E
Targeting individual subunits of the FokI restriction endonuclease to specific DNA strands.
Nucleic acids research 2009;37(7):2105-15.
2008: Bellamy Stuart R W; Mina Petros; Retter Susan E; Halford Stephen E
Fidelity of DNA sequence recognition by the SfiI restriction endonuclease is determined by communications between its two DNA-binding sites.
Journal of molecular biology 2008;384(3):557-63.
2008: Catto Lucy E; Bellamy Stuart R W; Retter Susan E; Halford Stephen E
Dynamics and consequences of DNA looping by the FokI restriction endonuclease.
Nucleic acids research 2008;36(6):2073-81.
2007: Bellamy Stuart R W; Milsom Susan E; Kovacheva Yana S; Sessions Richard B; Halford Stephen E
A switch in the mechanism of communication between the two DNA-binding sites in the SfiI restriction endonuclease.
Journal of molecular biology 2007;373(5):1169-83.
2007: Marshall Jacqueline J T; Gowers Darren M; Halford Stephen E
Restriction endonucleases that bridge and excise two recognition sites from DNA.
Journal of molecular biology 2007;367(2):419-31.
2006: Murphy Tanya A; Catto Lucy E; Halford Stephen E; Hadfield Andrea T; Minor Wladek; Walsh Timothy R; Spencer James
Crystal structure of Pseudomonas aeruginosa SPM-1 provides insights into variable zinc affinity of metallo-beta-lactamases.
Journal of molecular biology 2006;357(3):890-903.
2006: Catto Lucy E; Ganguly Sumita; Milsom Susan E; Welsh Abigail J; Halford Stephen E
Protein assembly and DNA looping by the FokI restriction endonuclease.
Nucleic acids research 2006;34(6):1711-20.
2005: Gowers Darren M; Wilson Geoffrey G; Halford Stephen E
Measurement of the contributions of 1D and 3D pathways to the translocation of a protein along DNA.
Proceedings of the National Academy of Sciences of the United States of America 2005;102(44):15883-8.
2005: Wood Katie M; Daniels Lucy E; Halford Stephen E
Long-range communications between DNA sites by the dimeric restriction endonuclease SgrAI.
Journal of molecular biology 2005;350(2):240-53.
2005: Bellamy Stuart R W; Milsom Susan E; Scott David J; Daniels Lucy E; Wilson Geoffrey G; Halford Stephen E
Cleavage of individual DNA strands by the different subunits of the heterodimeric restriction endonuclease BbvCI.
Journal of molecular biology 2005;348(3):641-53.
2004: Embleton Michelle L; Vologodskii Alexander V; Halford Stephen E
Dynamics of DNA loop capture by the SfiI restriction endonuclease on supercoiled and relaxed DNA.
Journal of molecular biology 2004;339(1):53-66.
2004: Gowers Darren M; Bellamy Stuart R W; Halford Stephen E
One recognition sequence, seven restriction enzymes, five reaction mechanisms.
Nucleic acids research 2004;32(11):3469-79.
2004: Halford Stephen E; Marko John F
How do site-specific DNA-binding proteins find their targets?
Nucleic acids research 2004;32(10):3040-52.
2004: Halford Stephen E; Welsh Abigail J; Szczelkun Mark D
Enzyme-mediated DNA looping.
Annual review of biophysics and biomolecular structure 2004;33():1-24.
2003: Kingston Isabel J; Gormley Niall A; Halford Stephen E
DNA supercoiling enables the type IIS restriction enzyme BspMI to recognise the relative orientation of two DNA sequences.
Nucleic acids research 2003;31(18):5221-8.
2003: Daniels Lucy E; Wood Katie M; Scott David J; Halford Stephen E
Subunit assembly for DNA cleavage by restriction endonuclease SgrAI.
Journal of molecular biology 2003;327(3):579-91.
2003: Gowers Darren M; Halford Stephen E
Protein motion from non-specific to specific DNA by three-dimensional routes aided by supercoiling.
The EMBO journal 2003;22(6):1410-8.
2002: Halford Stephen E; Szczelkun Mark D
How to get from A to B: strategies for analysing protein motion on DNA.
European biophysics journal : EBJ 2002;31(4):257-67.
2002: Simm Alan M; Higgins Catherine S; Carenbauer Anne L; Crowder Michael W; Bateson John H; Bennett Peter M; Clarke Anthony R; Halford Stephen E; Walsh Timothy R
Characterization of monomeric L1 metallo-beta -lactamase and the role of the N-terminal extension in negative cooperativity and antibiotic hydrolysis.
The Journal of biological chemistry 2002;277(27):24744-52.
2002: Williams Shelley A; Halford Stephen E
Communications between catalytic sites in the protein-DNA synapse by the SfiI endonuclease.
Journal of molecular biology 2002;318(2):387-94.
2002: Gormley Niall A; Hillberg Anna L; Halford Stephen E
The type IIs restriction endonuclease BspMI is a tetramer that acts concertedly at two copies of an asymmetric DNA sequence.
The Journal of biological chemistry 2002;277(6):4034-41.
2002: Bath Abigail J; Milsom Susan E; Gormley Niall A; Halford Stephen E
Many type IIs restriction endonucleases interact with two recognition sites before cleaving DNA.
The Journal of biological chemistry 2002;277(6):4024-33.
2001: Halford S E
Hopping, jumping and looping by restriction enzymes.
Biochemical Society transactions 2001;29(Pt 4):363-74.
2001: Milsom S E; Halford S E; Embleton M L; Szczelkun M D
Analysis of DNA looping interactions by type II restriction enzymes that require two copies of their recognition sites.
Journal of molecular biology 2001;311(3):515-27.
2001: Embleton M L; Siksnys V; Halford S E
DNA cleavage reactions by type II restriction enzymes that require two copies of their recognition sites.
Journal of molecular biology 2001;311(3):503-14.
2001: Williams S A; Halford S E
SfiI endonuclease activity is strongly influenced by the non-specific sequence in the middle of its recognition site.
Nucleic acids research 2001;29(7):1476-83.
2000: Stanford N P; Szczelkun M D; Marko J F; Halford S E
One- and three-dimensional pathways for proteins to reach specific DNA sites.
The EMBO journal 2000;19(23):6546-57.
2000: Halford S E; Gowers D M; Sessions R B
Two are better than one.
Nature structural biology 2000;7(9):705-7.
2000: Watson M A; Gowers D M; Halford S E
Alternative geometries of DNA looping: an analysis using the SfiI endonuclease.
Journal of molecular biology 2000;298(3):461-75.
2000: Gormley N A; Bath A J; Halford S E
Reactions of BglI and other type II restriction endonucleases with discontinuous recognition sites.
The Journal of biological chemistry 2000;275(10):6928-36.
2000: Baldwin G S; Gormley N A; Halford S E
Manganese(II) as a probe for the mechanism and specificity of restriction endonucleases.
Metal ions in biological systems 2000;37():345-64.
1999: Bilcock D T; Daniels L E; Bath A J; Halford S E
Reactions of type II restriction endonucleases with 8-base pair recognition sites.
The Journal of biological chemistry 1999;274(51):36379-86.
1999: Thomas M P; Brady R L; Halford S E; Sessions R B; Baldwin G S
Structural analysis of a mutational hot-spot in the EcoRV restriction endonuclease: a catalytic role for a main chain carbonyl group.
Nucleic acids research 1999;27(17):3438-45.
1999: Halford S E; Bilcock D T; Stanford N P; Williams S A; Milsom S E; Gormley N A; Watson M A; Bath A J; Embleton M L; Gowers D M; Daniels L E; Parry S H; Szczelkun M D
Restriction endonuclease reactions requiring two recognition sites.
Biochemical Society transactions 1999;27(4):696-9.
1999: Embleton M L; Williams S A; Watson M A; Halford S E
Specificity from the synapsis of DNA elements by the Sfi I endonuclease.
Journal of molecular biology 1999;289(4):785-97.
1999: Stanford N P; Halford S E; Baldwin G S
DNA cleavage by the EcoRV restriction endonuclease: pH dependence and proton transfers in catalysis.
Journal of molecular biology 1999;288(1):105-16.
1999: Baldwin G S; Sessions R B; Erskine S G; Halford S E
DNA cleavage by the EcoRV restriction endonuclease: roles of divalent metal ions in specificity and catalysis.
Journal of molecular biology 1999;288(1):87-103.
1999: Mizuuchi K; Nobbs T J; Halford S E; Adzuma K; Qin J
A new method for determining the stereochemistry of DNA cleavage reactions: application to the SfiI and HpaII restriction endonucleases and to the MuA transposase.
Biochemistry 1999;38(14):4640-8.
1999: Bilcock D T; Halford S E
DNA restriction dependent on two recognition sites: activities of the SfiI restriction-modification system in Escherichia coli.
Molecular microbiology 1999;31(4):1243-54.
1998: Wentzell L M; Halford S E
DNA looping by the Sfi I restriction endonuclease.
Journal of molecular biology 1998;281(3):433-44.
1998: Nobbs T J; Szczelkun M D; Wentzell L M; Halford S E
DNA excision by the Sfi I restriction endonuclease.
Journal of molecular biology 1998;281(3):419-32.
1998: Nobbs T J; Williams S A; Connolly B A; Halford S E
Phosphorothioate substrates for the SfiI restriction endonuclease.
Biological chemistry 1998;379(4-5):599-604.
1998: Erskine S G; Halford S E
Reactions of the eco RV restriction endonuclease with fluorescent oligodeoxynucleotides: identical equilibrium constants for binding to specific and non-specific DNA.
Journal of molecular biology 1998;275(5):759-72.
1997: Szczelkun M D; Janscak P; Firman K; Halford S E
Selection of non-specific DNA cleavage sites by the type IC restriction endonuclease EcoR124I.
Journal of molecular biology 1997;271(1):112-23.
1997: Sessions R B; Oram M; Szczelkun M D; Halford S E
Random walk models for DNA synapsis by resolvase.
Journal of molecular biology 1997;270(3):413-25.
1997: Oram M; Marko J F; Halford S E
Communications between distant sites on supercoiled DNA from non-exponential kinetics for DNA synapsis by resolvase.
Journal of molecular biology 1997;270(3):396-412.
1997: Hancox E L; Halford S E
Kinetic analysis of a mutational hot spot in the EcoRV restriction endonuclease.
Biochemistry 1997;36(24):7577-85.
1997: Erskine S G; Baldwin G S; Halford S E
Rapid-reaction analysis of plasmid DNA cleavage by the EcoRV restriction endonuclease.
Biochemistry 1997;36(24):7567-76.
1996: Szczelkun M D; Dillingham M S; Janscak P; Firman K; Halford S E
Repercussions of DNA tracking by the type IC restriction endonuclease EcoR124I on linear, circular and catenated substrates.
The EMBO journal 1996;15(22):6335-47.
1996: Szczelkun M D; Halford S E
Recombination by resolvase to analyse DNA communications by the SfiI restriction endonuclease.
The EMBO journal 1996;15(6):1460-9.
1996: Vipond I B; Moon B J; Halford S E
An isoleucine to leucine mutation that switches the cofactor requirement of the EcoRV restriction endonuclease from magnesium to manganese.
Biochemistry 1996;35(6):1712-21.
1996: Vipond I B; Halford S E
Random mutagenesis targeted to the active site of the EcoRV restriction endonuclease.
Biochemistry 1996;35(6):1701-11.
1995: Vipond I B; Baldwin G S; Oram M; Erskine S G; Wentzell L M; Szczelkun M D; Nobbs T J; Halford S E
A general assay for restriction endonucleases and other DNA-modifying enzymes with plasmid substrates.
Molecular biotechnology 1995;4(3):259-68.
1995: Oram M; Szczelkun M D; Halford S E
Recombination. Pieces of the site-specific recombination puzzle.
Current biology : CB 1995;5(10):1106-9.
1995: Nobbs T J; Halford S E
DNA cleavage at two recognition sites by the SfiI restriction endonuclease: salt dependence of cis and trans interactions between distant DNA sites.
Journal of molecular biology 1995;252(4):399-411.
1995: Wentzell L M; Nobbs T J; Halford S E
The SfiI restriction endonuclease makes a four-strand DNA break at two copies of its recognition sequence.
Journal of molecular biology 1995;248(3):581-95.
1995: Erskine S G; Halford S E
Interactions of the EcoRV restriction endonuclease with fluorescent oligodeoxynucleotides.
Gene 1995;157(1-2):153-6.
1995: Soultanas P; Halford S E
Site-specific recombination at res sites containing DNA-binding sequences for both Tn21 resolvase and CAP.
Journal of molecular biology 1995;245(3):219-27.
1995: Soultanas P; Oram M; Halford S E
Site-specific recombination at res sites containing DNA-binding sequences for both Tn21 and Tn3 resolvases.
Journal of molecular biology 1995;245(3):208-18.
1995: Vipond I B; Halford S E
Specific DNA recognition by EcoRV restriction endonuclease induced by calcium ions.
Biochemistry 1995;34(4):1113-9.
1995: Baldwin G S; Vipond I B; Halford S E
Rapid reaction analysis of the catalytic cycle of the EcoRV restriction endonuclease.
Biochemistry 1995;34(2):705-14.
1995: Vipond I B; Baldwin G S; Halford S E
Divalent metal ions at the active sites of the EcoRV and EcoRI restriction endonucleases.
Biochemistry 1995;34(2):697-704.
1994: Oram M; Shipstone E; Halford S E
Synapsis by Tn3 resolvase: speed and dependence on DNA supercoiling.
Biochemical Society transactions 1994;22(3):303S.
1994: Wentzell L M; Oram M; Halford S E
Purification and characterisation of the SfiI restriction endonuclease.
Biochemical Society transactions 1994;22(3):302S.
1994: Vipond I B; Moon B J; Halford S E
An Ile-to-Leu mutant of the EcoRV restriction endonuclease that requires Mn2+ as catalytic cofactor.
Biochemical Society transactions 1994;22(3):301S.
1994: Baldwin G S; Halford S E
Rapid reaction kinetics on the EcoRV restriction endonuclease.
Biochemical Society transactions 1994;22(3):300S.
1994: Erskine S G; Halford S E
Fluorescent substrates for the EcoRV restriction endonuclease.
Biochemical Society transactions 1994;22(3):299S.
1994: Stark W M; Parker C N; Halford S E; Boocock M R
Stereoselectivity of DNA catenane fusion by resolvase.
Nature 1994;368(6466):76-8.
1994: Halford S E; Taylor J D; Vermote C L; Vipond I B
Assays for restriction endonucleases using plasmid substrates.
Methods in molecular biology (Clifton, N.J.) 1994;30():385-96.
1994: Taylor J D; Ackroyd A J; Halford S E
The gel shift assay for the analysis of DNA-protein interactions.
Methods in molecular biology (Clifton, N.J.) 1994;30():263-79.
1993: Hall S C; Halford S E
Specificity of DNA recognition in the nucleoprotein complex for site-specific recombination by Tn21 resolvase.
Nucleic acids research 1993;21(24):5712-9.
1993: Vipond I B; Halford S E
Structure-function correlation for the EcoRV restriction enzyme: from non-specific binding to specific DNA cleavage.
Molecular microbiology 1993;9(2):225-31.
1992: Vermote C L; Vipond I B; Halford S E
EcoRV restriction endonuclease: communication between DNA recognition and catalysis.
Biochemistry 1992;31(26):6089-97.
1992: Vermote C L; Halford S E
EcoRV restriction endonuclease: communication between catalytic metal ions and DNA recognition.
Biochemistry 1992;31(26):6082-9.
1992: Taylor J D; Halford S E
The activity of the EcoRV restriction endonuclease is influenced by flanking DNA sequences both inside and outside the DNA-protein complex.
Biochemistry 1992;31(1):90-7.
1991: Taylor J D; Badcoe I G; Clarke A R; Halford S E
EcoRV restriction endonuclease binds all DNA sequences with equal affinity.
Biochemistry 1991;30(36):8743-53.
1991: Parker C N; Halford S E
Dynamics of long-range interactions on DNA: the speed of synapsis during site-specific recombination by resolvase.
Cell 1991;66(4):781-91.
1990: Taylor J D; Goodall A J; Vermote C L; Halford S E
Fidelity of DNA recognition by the EcoRV restriction/modification system in vivo.
Biochemistry 1990;29(48):10727-33.
1990: Avila P; Ackroyd A J; Halford S E
DNA binding by mutants of Tn21 resolvase with DNA recognition functions from Tn3 resolvase.
Journal of molecular biology 1990;216(3):645-55.
1990: Ackroyd A J; Avila P; Parker C N; Halford S E
Site-specific recombination by mutants of Tn21 resolvase with DNA recognition functions from Tn3 resolvase.
Journal of molecular biology 1990;216(3):633-43.
1989: Castell S E; Halford S E
DNA supercoiling determines the activation energy barrier for site specific recombination by Tn21 resolvase.
Nucleic acids research 1989;17(17):7045-58.
1989: Taylor J D; Halford S E
Discrimination between DNA sequences by the EcoRV restriction endonuclease.
Biochemistry 1989;28(15):6198-207.
1989: Bennett S P; Halford S E
Recognition of DNA by type II restriction enzymes.
Current topics in cellular regulation 1989;30():57-104.
1988: Garnett J; Halford S E
Properties and subunit structure of EcoRV methyltransferase.
Gene 1988;74(1):73-6.
1988: Halford S E; Goodall A J
Modes of DNA cleavage by the EcoRV restriction endonuclease.
Biochemistry 1988;27(5):1771-7.

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