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Stephen Harding

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Richard B Gillis; Gary G Adams; Monica Berry; Tabot M D Besong; Anthony Corfield; Samil M Kök; David Lafond; Arthur J Rowe; Ray Sidebottom; Bettina Wolf; Stephen E Harding
Molecular weight distribution analysis by ultracentrifugation: adaptation of a new approach for mucins.
Ali Saber Abdelhameed; Gary G Adams; Benjamin Bonnier; Louis Cerny; Karel Conrath; Pierre Duvivier; Olivier Laloux; Christophe Lenfant; Gordon A Morris; Arthur J Rowe; Stephen E Harding
An asymmetric and slightly dimerized structure for the tetanus toxoid protein used in glycoconjugate vaccines.
Stephen E Harding
Meeting report: the stability and degradation of complex carbohydrate structures: mechanisms and measurement.

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2013: Richard B Gillis; Gary G Adams; Monica Berry; Tabot M D Besong; Anthony Corfield; Samil M Kök; David Lafond; Arthur J Rowe; Ray Sidebottom; Bettina Wolf; Stephen E Harding
Molecular weight distribution analysis by ultracentrifugation: adaptation of a new approach for mucins.
Carbohydrate polymers 2013;93(1):178-83.
2012: Ali Saber Abdelhameed; Gary G Adams; Benjamin Bonnier; Louis Cerny; Karel Conrath; Pierre Duvivier; Olivier Laloux; Christophe Lenfant; Gordon A Morris; Arthur J Rowe; Stephen E Harding
An asymmetric and slightly dimerized structure for the tetanus toxoid protein used in glycoconjugate vaccines.
Carbohydrate polymers 2012;90(4):1831-5.
2012: Stephen E Harding
Meeting report: the stability and degradation of complex carbohydrate structures: mechanisms and measurement.
Biotechnology & genetic engineering reviews 2012;28():177-82.
2012: Gary G Adams; Stephen E Harding; Mohammad Ilyas; Shahwar Imran; M Samil Kök; Arthur S Tatham
The interaction of dietary fibres with disulphide bonds (S-S) and a potential strategy to reduce the toxicity of the gluten proteins in coeliac disease.
Biotechnology & genetic engineering reviews 2012;28():115-30.
2012: Shahwar Imran; M Samil Kok; Richard B Gillis; Stephen E Harding; Gary G Adams
Application and use of Inulin as a tool for therapeutic drug delivery.
Biotechnology & genetic engineering reviews 2012;28():33-45.
2011: Trushar R Patel; Donald J Winzor; Tabot M D Besong; Stephen E Harding; Markus Meier; Nehal Patel; Jörg Stetefeld
Evidence for self-association of a miniaturized version of agrin from hydrodynamic and small-angle X-ray scattering measurements.
The journal of physical chemistry. B 2011;115(38):11286-93.
2011: Thomas Heinze; Nico Michaelis; Gordon A Morris; Melanie Nikolajski; Arthur J Rowe; Peter Berlin; Tabot M D Besong; Stephan Daus; Stephen E Harding
Protein-like oligomerization of carbohydrates.
Angewandte Chemie (International ed. in English) 2011;50(37):8602-4.
2011: Stephen E Harding; Peter Schuck; Ali Saber Abdelhameed; Gary Adams; M Samil Kök; Gordon A Morris
Extended Fujita approach to the molecular weight distribution of polysaccharides and other polymeric systems.
Methods (San Diego, Calif.) 2011;54(1):136-44.
2010: Stephen E Harding
Some observations on the effects of bioprocessing on biopolymer stability.
Journal of drug targeting 2010;18(10):732-40.
2010: Trushar R Patel; Gordon A Morris; Daniela Zwolanek; Douglas R Keene; Jianhua Li; Stephen E Harding; Manuel Koch; Jörg Stetefeld
Nano-structure of the laminin γ-1 short arm reveals an extended and curved multidomain assembly.
Matrix biology : journal of the International Society for Matrix Biology 2010;29(7):565-72.
2010: Stephen E Harding; Arthur J Rowe
Insight into protein-protein interactions from analytical ultracentrifugation.
Biochemical Society transactions 2010;38(4):901-7.
2010: Stephen E Harding; Ali Abdelhameed; Gordon A Morris
Molecular weight distribution evaluation of polysaccharides and glycoconjugates using analytical ultracentrifugation.
Macromolecular bioscience 2010;10(7):714-20.
2010: D Amorós; A Ortega; Stephen E Harding; José García de la Torre
Multi-scale calculation and global-fit analysis of hydrodynamic properties of biological macromolecules: determination of the overall conformation of antibody IgG molecules.
European biophysics journal : EBJ 2010;39(3):361-70.
2008: Trushar R Patel; Gordon A Morris; Jose Garcia de la Torre; Alvaro Ortega; Petra Mischnick; Stephen E Harding
Molecular flexibility of methylcelluloses of differing degree of substitution by combined sedimentation and viscosity analysis.
Macromolecular bioscience 2008;8(12):1108-15.
2008: Marit Inngjerdingen; Kari Inngjerdingen; Trushar R Patel; Stephanie Allen; Xinyong Chen; Bent Rolstad; Gordon A Morris; Stephen E Harding; Terje E Michaelsen; Drissa Diallo; Berit Smestad Paulsen
Pectic polysaccharides from Biophytum petersianum Klotzsch, and their activation of macrophages and dendritic cells.
Glycobiology 2008;18(12):1074-84.
2008: Yanling Lu; Stephen E Harding; Alison Turner; Bryan Smith; Diljeet S Athwal; J Günter Grossmann; Kenneth G Davis; Arthur J Rowe
Effect of PEGylation on the solution conformation of antibody fragments.
Journal of pharmaceutical sciences 2008;97(6):2062-79.
2008: Georgina R Bowden; Patricia Balaresque; Turi E King; Ziff Hansen; Andrew C Lee; Giles Pergl-Wilson; Emma Hurley; Stephen J Roberts; Patrick Waite; Judith Jesch; Abigail L Jones; Mark Thomas; Stephen E Harding; Mark A Jobling
Excavating past population structures by surname-based sampling: the genetic legacy of the Vikings in northwest England.
Molecular biology and evolution 2008;25(2):301-9.
2008: Yanling Lu; Stephen E Harding; Arthur J Rowe; Kenneth G Davis; Brendan Fish; Paul Varley; C Gee; S Mulot
The effect of a point mutation on the stability of IgG4 as monitored by analytical ultracentrifugation.
Journal of pharmaceutical sciences 2008;97(2):960-9.
2008: Andreas Koschella; Kari Inngjerdingen; Berit Smestad Paulsen; Gordon A Morris; Stephen E Harding; Thomas Heinze
Unconventional methyl galactan synthesized via the thexyldimethylsilyl intermediate: preparation, characterization, and properties.
Macromolecular bioscience 2008;8(1):96-105.
2007: Kari Inngjerdingen; Trushar R Patel; Xinyong Chen; Lennart Kenne; Stephanie Allen; Gordon A Morris; Stephen E Harding; Tsukasa Matsumoto; Drissa Diallo; Haruki Yamada; Terje E Michaelsen; Marit Inngjerdingen; Berit Smestad Paulsen
Immunological and structural properties of a pectic polymer from Glinus oppositifolius.
Glycobiology 2007;17(12):1299-310.
2007: Yanling Lu; Stephen E Harding; Terje E Michaelsen; Emma Longman; Kenneth G Davis; Alvaro Ortega; J Günter Grossmann; Inger Sandlie; José García de la Torre
Solution conformation of wild-type and mutant IgG3 and IgG4 immunoglobulins using crystallohydrodynamics: possible implications for complement activation.
Biophysical journal 2007;93(11):3733-44.
2007: Trushar R Patel; Stephen E Harding; Anna Ebringerova; Marcin Deszczynski; Zdenka Hromadkova; Adiaratou Togola; Berit Smestad Paulsen; Gordon A Morris; Arthur J Rowe
Weak self-association in a carbohydrate system.
Biophysical journal 2007;93(3):741-9.
2007: Donald J Winzor; Marcin Deszczynski; Stephen E Harding; Peter R Wills
Nonequivalence of second virial coefficients from sedimentation equilibrium and static light scattering studies of protein solutions.
Biophysical chemistry 2007;128(1):46-55.
2007: Ulf Nobbmann; Malcolm Connah; Brendan Fish; Paul Varley; C Gee; S Mulot; Juntao Chen; Liang Zhou; Yanling Lu; Fei Shen; Junming Yi; Stephen E Harding
Dynamic light scattering as a relative tool for assessing the molecular integrity and stability of monoclonal antibodies.
Biotechnology & genetic engineering reviews 2007;24():117-28.
2006: Trushar R Patel; David R Picout; Simon B Ross-Murphy; Stephen E Harding
Pressure cell assisted solution characterization of galactomannans. 3. Application of analytical ultracentrifugation techniques.
Biomacromolecules 2006;7(12):3513-20.
2006: Yanling Lu; Emma Longman; Kenneth G Davis; Alvaro Ortega; J Günter Grossmann; Terje E Michaelsen; José García de la Torre; Stephen E Harding
Crystallohydrodynamics of protein assemblies: Combining sedimentation, viscometry, and x-ray scattering.
Biophysical journal 2006;91(5):1688-97.
2006: Marcin Deszczynski; Stephen E Harding; Donald J Winzor
Negative second virial coefficients as predictors of protein crystal growth: evidence from sedimentation equilibrium studies that refutes the designation of those light scattering parameters as osmotic virial coefficients.
Biophysical chemistry 2006;120(2):106-13.
2005: Kari Inngjerdingen; Sylvi C Debes; Marit Inngjerdingen; Sanya Hokputsa; Stephen E Harding; Bent Rolstad; Terje E Michaelsen; Drissa Diallo; Berit Smestad Paulsen
Bioactive pectic polysaccharides from Glinus oppositifolius (L.) Aug. DC., a Malian medicinal plant, isolation and partial characterization.
Journal of ethnopharmacology 2005;101(1-3):204-14.
2005: Cecilie Sogn Nergard; Tsukasa Matsumoto; Marit Inngjerdingen; Kari Inngjerdingen; Sanya Hokputsa; Stephen E Harding; Terje E Michaelsen; Drissa Diallo; Hiroaki Kiyohara; Berit Smestad Paulsen; Haruki Yamada
Structural and immunological studies of a pectin and a pectic arabinogalactan from Vernonia kotschyana Sch. Bip. ex Walp. (Asteraceae).
Carbohydrate research 2005;340(1):115-30.
2004: Donald J Winzor; Lyle E Carrington; Marcin Deszczynski; Stephen E Harding
Extent of charge screening in aqueous polysaccharide solutions.
Biomacromolecules 2004;5(6):2456-60.
2004: Donald J Winzor; Susan Jones; Stephen E Harding
Determination of protein charge by capillary zone electrophoresis.
Analytical biochemistry 2004;333(2):225-9.
2004: Donald J Winzor; Lyle E Carrington; Stephen E Harding
Limitations of the ultracentrifugal approach for measuring the effective net charge of a macroion.
Analytical biochemistry 2004;333(1):114-8.
2004: Sanya Hokputsa; Stephen E Harding; Kari Inngjerdingen; Kornelia Jumel; Terje E Michaelsen; Thomas Heinze; Andreas Koschella; Berit Smestad Paulsen
Bioactive polysaccharides from the stems of the Thai medicinal plant Acanthus ebracteatus: their chemical and physical features.
Carbohydrate research 2004;339(4):753-62.
2004: Stephen E Harding; Emma Longman; Beatriz Carrasco; Alvaro Ortega; Jose Garcia de la Torre
Studying antibody conformations by ultracentrifugation and hydrodynamic modeling.
Methods in molecular biology (Clifton, N.J.) 2004;248():93-113.
2003: Graham W Pettigrew; Sofia pauleta; Celia F Goodhew; Alan Cooper; Margaret Nutley; Kornelia Jumel; Stephen E Harding; Cristina Costa; Ludwig Krippahl; Isabel Moura; Jose Moura
Electron transfer complexes of cytochrome c peroxidase from Paracoccus denitrificans containing more than one cytochrome.
Biochemistry 2003;42(41):11968-81.
2003: Stephen E Harding
Mucoadhesive interactions.
Biochemical Society transactions 2003;31(Pt 5):1036-41.
2003: Monica Fee; Neil Errington; Kornelia Jumel; Lisbeth Illum; Alan Smith; Stephen E Harding
Correlation of SEC/MALLS with ultracentrifuge and viscometric data for chitosans.
European biophysics journal : EBJ 2003;32(5):457-64.
2003: Emma Longman; Katja Kreusel; Saul B Tendler; Immo Fiebrig; Kevin King; John Adair; Paul O'Shea; Alvaro Ortega; Jose Garcia de la Torre; Stephen E Harding
Estimating domain orientation of two human antibody IgG4 chimeras by crystallohydrodynamics.
European biophysics journal : EBJ 2003;32(5):503-10.
2003: Sanya Hokputsa; Kornelia Jumel; Catherine Alexander; Stephen E Harding
A comparison of molecular mass determination of hyaluronic acid using SEC/MALLS and sedimentation equilibrium.
European biophysics journal : EBJ 2003;32(5):450-6.
2003: David R Picout; Simon B Ross-Murphy; Neil Errington; Stephen E Harding
Pressure cell assisted solubilization of xyloglucans: tamarind seed polysaccharide and detarium gum.
Biomacromolecules 2003;4(3):799-807.
2003: M Ines Borges-Walmsley; Jeremy Beauchamp; Sharon M Kelly; Kornelia Jumel; Denise Candlish; Stephen E Harding; Nicholas C Price; Adrian R Walmsley
Identification of oligomerization and drug-binding domains of the membrane fusion protein EmrA.
The Journal of biological chemistry 2003;278(15):12903-12.
2003: Graham W Pettigrew; Celia F Goodhew; Alan Cooper; Margaret Nutley; Kornelia Jumel; Stephen E Harding
The electron transfer complexes of cytochrome c peroxidase from Paracoccus denitrificans.
Biochemistry 2003;42(7):2046-55.
2002: David R Picout; Simon B Ross-Murphy; Kornelia Jumel; Stephen E Harding
Pressure cell assisted solution characterization of polysaccharides. 2. Locust bean gum and tara gum.
Biomacromolecules 2002;3(4):761-7.
2002: Christopher Walters; Neil Errington; Arthur J Rowe; Stephen E Harding
Hydrolysable ATP is a requirement for the correct interaction of molecular chaperonins cpn60 and cpn10.
The Biochemical journal 2002;364(Pt 3):849-55.
2001: Donald J Winzor; Lyle E Carrington; Stephen E Harding
A potential role for the analytical ultracentrifuge in the experimental measurement of protein valence.
Analytical biochemistry 2001;299(2):235-40.
2001: Donald J Winzor; Lyle E Carrington; Stephen E Harding
Analysis of thermodynamic non-ideality in terms of protein solvation.
Biophysical chemistry 2001;93(2-3):231-40.
2001: Beatriz Carrasco; Jose Garcia de la Torre; K G Davis; S Jones; D Athwal; C Walters; D R Burton; Stephen E Harding
Crystallohydrodynamics for solving the hydration problem for multi-domain proteins: open physiological conformations for human IgG.
Biophysical chemistry 2001;93(2-3):181-96.
2001: Stephen E Harding
The hydration problem in solution biophysics: an introduction.
Biophysical chemistry 2001;93(2-3):87-91.
2001: David R Picout; Simon B Ross-Murphy; N Errington; Stephen E Harding
Pressure cell assisted solution characterization of polysaccharides. 1. Guar gum.
Biomacromolecules 2001;2(4):1301-9.
2000: C Walters; A Clarke; Matthew J Cliff; Peter Lund; Stephen E Harding
Trp203 mutation in GroEL promotes a self-association reaction: a hydrodynamic study.
European biophysics journal : EBJ 2000;29(6):420-8.
1999: Beatriz Carrasco; José García de la Torre; O Byron; D King; C Walters; S Jones; Stephen E Harding
Novel size-independent modeling of the dilute solution conformation of the immunoglobulin IgG Fab' domain using SOLPRO and ELLIPS.
Biophysical journal 1999;77(6):2902-10.
1999: DA Schiffmann; J H White; Alan Cooper; Margaret Nutley; Stephen E Harding; K Jumel; R Solari; Keith Ray; nicholas gay
Formation and biochemical characterization of tube/pelle death domain complexes: critical regulators of postreceptor signaling by the Drosophila toll receptor.
Biochemistry 1999;38(36):11722-33.
1999: Stephen E Harding; J C Horton; S Jones; J M Thornton; Donald J Winzor
COVOL: an interactive program for evaluating second virial coefficients from the triaxial shape or dimensions of rigid macromolecules.
Biophysical journal 1999;76(5):2432-8.
1999: José García de la Torre; Stephen E Harding; Beatriz Carrasco
Calculation of NMR relaxation, covolume, and scattering-related properties of bead models using the SOLPRO computer program.
European biophysics journal : EBJ 1999;28(2):119-32.
1999: S Dodd; G A Place; R L Hall; Stephen E Harding
Hydrodynamic properties of mucins secreted by primary cultures of guinea-pig tracheal epithelial cells: determination of diffusion coefficients by analytical ultracentrifugation and kinetic analysis of mucus gel hydration and dissolution.
European biophysics journal : EBJ 1999;28(1):38-47.
1998: Stephen E Harding; J C Horton; Donald J Winzor
COVOL: an answer to your thermodynamic non-ideality problems?
Biochemical Society transactions 1998;26(4):737-41.
1998: Beatriz Carrasco; José García de la Torre; Stephen E Harding
Models for the multisubunit conformation of oil-seed globulins.
Biochemical Society transactions 1998;26(4):721-5.
1998: José García de la Torre; Stephen E Harding; Beatriz Carrasco
Advances in modelling solution properties of macromolecules and particles.
Biochemical Society transactions 1998;26(4):716-21.
1998: Beatriz Carrasco; Stephen E Harding; José García de la Torre
Bead modeling using HYDRO and SOLPRO of the conformation of multisubunit proteins: sunflower and rape-seed 11S globulins.
Biophysical chemistry 1998;74(2):127-33.
1997: Kornelia Jumel; K Geider; Stephen E Harding
The solution molecular weight and shape of the bacterial exopolysaccharides amylovoran and stewartan.
International journal of biological macromolecules 1997;20(4):251-8.
1997: EK Wilson; Nigel S Scrutton; H Cölfen; Stephen E Harding; M P Jacobsen; Donald J Winzor
An ultracentrifugal approach to quantitative characterization of the molecular assembly of a physiological electron-transfer complex: the interaction of electron-transferring flavoprotein with trimethylamine dehydrogenase.
European journal of biochemistry / FEBS 1997;243(1-2):393-9.
1997: José García de la Torre; Beatriz Carrasco; Stephen E Harding
SOLPRO: theory and computer program for the prediction of SOLution PROperties of rigid macromolecules and bioparticles.
European biophysics journal : EBJ 1997;25(5-6):361-72.
1997: Stephen E Harding; J C Horton; H Cölfen
The ELLIPS suite of macromolecular conformation algorithms.
European biophysics journal : EBJ 1997;25(5-6):347-59.
1997: Kornelia Jumel; F J Fogg; David A Hutton; J P Pearson; A Allen; Stephen E Harding
A polydisperse linear random coil model for the quaternary structure of pig colonic mucin.
European biophysics journal : EBJ 1997;25(5-6):477-80.
1997: H Silkowski; S J Davis; A N Barclay; Arthur J Rowe; Stephen E Harding; Olwyn Byron
Characterisation of the low affinity interaction between rat cell adhesion molecules CD2 and CD48 by analytical ultracentrifugation.
European biophysics journal : EBJ 1997;25(5-6):455-62.
1992: A D Molina-Garcia; Stephen E Harding; F G Diaz; J G de la Torre; D Rowitch; Richard N Perham
Effect of coat protein mutations in bacteriophage fd studied by sedimentation analysis.
Biophysical journal 1992;63(5):1293-8.