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Wataru Hashimoto

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Bunzo Mikami; Osamu Miyake; Mizuho Ban; Wataru Hashimoto; Yukie Maruyama; Kohei Ogura; Sachiko Suzuki; Masayuki Yamasaki; Hye-Jin Yoon; Kousaku Murata
Induced-fit motion of a lid loop involved in catalysis in alginate lyase A1-III.
Yu Nishitani; Wataru Hashimoto; Takafumi Itoh; Yukie Maruyama; Bunzo Mikami; Kousaku Murata
Recognition of heteropolysaccharide alginate by periplasmic solute-binding proteins of a bacterial ABC transporter.
Yukie Maruyama; Bunzo Mikami; Wataru Hashimoto; Takafumi Itoh; Yu Nishitani; Kousaku Murata
Crystallization and preliminary X-ray analysis of alginate importer from Sphingomonas sp. A1.

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2012: Bunzo Mikami; Osamu Miyake; Mizuho Ban; Wataru Hashimoto; Yukie Maruyama; Kohei Ogura; Sachiko Suzuki; Masayuki Yamasaki; Hye-Jin Yoon; Kousaku Murata
Induced-fit motion of a lid loop involved in catalysis in alginate lyase A1-III.
Acta crystallographica. Section D, Biological crystallography 2012;68(Pt 9):1207-16.
2012: Yu Nishitani; Wataru Hashimoto; Takafumi Itoh; Yukie Maruyama; Bunzo Mikami; Kousaku Murata
Recognition of heteropolysaccharide alginate by periplasmic solute-binding proteins of a bacterial ABC transporter.
Biochemistry 2012;51(17):3622-33.
2012: Yukie Maruyama; Bunzo Mikami; Wataru Hashimoto; Takafumi Itoh; Yu Nishitani; Kousaku Murata
Crystallization and preliminary X-ray analysis of alginate importer from Sphingomonas sp. A1.
Acta crystallographica. Section F, Structural biology and crystallization communications 2012;68(Pt 3):317-20.
2011: Yukie Maruyama; Asako Chuma; Bunzo Mikami; Wataru Hashimoto; Kousaku Murata
Heterosubunit composition and crystal structures of a novel bacterial M16B metallopeptidase.
Journal of molecular biology 2011;407(1):180-92.
2011: Yukie Maruyama; Akihito Ochiai; Bunzo Mikami; Wataru Hashimoto; Kousaku Murata
Crystal structure of bacterial cell-surface alginate-binding protein with an M75 peptidase motif.
Biochemical and biophysical research communications 2011;405(3):411-6.
2010: Shigeyuki Kawai; Wataru Hashimoto; Kousaku Murata
Transformation of Saccharomyces cerevisiae and other fungi: methods and possible underlying mechanism.
Bioengineered bugs 2010;1(6):395-403.
2010: Ryuichi Takase; Akihito Ochiai; Bunzo Mikami; Wataru Hashimoto; Kousaku Murata
Molecular identification of unsaturated uronate reductase prerequisite for alginate metabolism in Sphingomonas sp. A1.
Biochimica et biophysica acta 2010;1804(9):1925-36.
2010: Yukie Maruyama; Akihito Ochiai; Takafumi Itoh; Bunzo Mikami; Wataru Hashimoto; Kousaku Murata
Mutational studies of the peptidoglycan hydrolase FlgJ of Sphingomonas sp. strain A1.
Journal of basic microbiology 2010;50(4):311-7.
2010: Wataru Hashimoto; Shigeyuki Kawai; Kousaku Murata
Bacterial supersystem for alginate import/metabolism and its environmental and bioenergy applications.
Bioengineered bugs 2010;1(2):97-109.
2009: Kohei Ogura; Masayuki Yamasaki; Takashi Yamada; Bunzo Mikami; Wataru Hashimoto; Kousaku Murata
Crystal structure of family 14 polysaccharide lyase with pH-dependent modes of action.
The Journal of biological chemistry 2009;284(51):35572-9.
2009: Wataru Hashimoto; Akihito Ochiai; Jinshan He; Takafumi Itoh; Bunzo Mikami; Kousaku Murata
Crystallization and preliminary crystallographic analysis of the cell-surface alginate-binding protein Algp7 isolated from Sphingomonas sp. A1.
Acta crystallographica. Section F, Structural biology and crystallization communications 2009;65(Pt 5):515-7.
2009: Akihito Ochiai; Takafumi Itoh; Bunzo Mikami; Wataru Hashimoto; Kousaku Murata
Structural determinants responsible for substrate recognition and mode of action in family 11 polysaccharide lyases.
The Journal of biological chemistry 2009;284(15):10181-9.
2009: Wataru Hashimoto; Akihito Ochiai; Keiko Momma; Takafumi Itoh; Bunzo Mikami; Yukie Maruyama; Kousaku Murata
Crystal structure of the glycosidase family 73 peptidoglycan hydrolase FlgJ.
Biochemical and biophysical research communications 2009;381(1):16-21.
2008: Jinshan He; Akihito Ochiai; Yasuki Fukuda; Wataru Hashimoto; Kousaku Murata
A putative lipoprotein of Sphingomonas sp. strain A1 binds alginate rather than a lipid moiety.
FEMS microbiology letters 2008;288(2):221-6.
2008: Kohei Ogura; Masayuki Yamasaki; Bunzo Mikami; Wataru Hashimoto; Kousaku Murata
Substrate recognition by family 7 alginate lyase from Sphingomonas sp. A1.
Journal of molecular biology 2008;380(2):373-85.
2008: Takafumi Itoh; Bunzo Mikami; Wataru Hashimoto; Kousaku Murata
Crystal structure of YihS in complex with D-mannose: structural annotation of Escherichia coli and Salmonella enterica yihS-encoded proteins to an aldose-ketose isomerase.
Journal of molecular biology 2008;377(5):1443-59.
2008: Yukie Maruyama; Misato Momma; Bunzo Mikami; Wataru Hashimoto; Kousaku Murata
Crystal structure of a novel bacterial cell-surface flagellin binding to a polysaccharide.
Biochemistry 2008;47(5):1393-402.
2008: Kousaku Murata; Shigeyuki Kawai; Bunzo Mikami; Wataru Hashimoto
Superchannel of bacteria: biological significance and new horizons.
Bioscience, biotechnology, and biochemistry 2008;72(2):265-77.
2008: Karen Mine Harada; Keiko Tanaka; Yasuki Fukuda; Wataru Hashimoto; Kousaku Murata
Paenibacillus sp. strain HC1 xylanases responsible for degradation of rice bran hemicellulose.
Microbiological research 2008;163(3):293-8.
2007: Akihito Ochiai; Takafumi Itoh; Yukie Maruyama; Akiko Kawamata; Bunzo Mikami; Wataru Hashimoto; Kousaku Murata
A novel structural fold in polysaccharide lyases: Bacillus subtilis family 11 rhamnogalacturonan lyase YesW with an eight-bladed beta-propeller.
The Journal of biological chemistry 2007;282(51):37134-45.
2007: Wataru Hashimoto; Takafumi Itoh; Yukie Maruyama; Bunzo Mikami; Kousaku Murata
Hydration of vinyl ether groups by unsaturated glycoside hydrolases and their role in bacterial pathogenesis.
International microbiology : the official journal of the Spanish Society for Microbiology 2007;10(4):233-43.
2007: Zhongli Cui; Yukie Maruyama; Bunzo Mikami; Wataru Hashimoto; Kousaku Murata
Crystal structure of glycoside hydrolase family 78 alpha-L-Rhamnosidase from Bacillus sp. GL1.
Journal of molecular biology 2007;374(2):384-98.
2007: Akihito Ochiai; Takafumi Itoh; Akiko Kawamata; Wataru Hashimoto; Kousaku Murata
Plant cell wall degradation by saprophytic Bacillus subtilis strains: gene clusters responsible for rhamnogalacturonan depolymerization.
Applied and environmental microbiology 2007;73(12):3803-13.
2007: Yukie Maruyama; Bunzo Mikami; Wataru Hashimoto; Kousaku Murata
A structural factor responsible for substrate recognition by Bacillus sp. GL1 xanthan lyase that acts specifically on pyruvated side chains of xanthan.
Biochemistry 2007;46(3):781-91.
2006: Takafumi Itoh; Wataru Hashimoto; Bunzo Mikami; Kousaku Murata
Crystal structure of unsaturated glucuronyl hydrolase complexed with substrate: molecular insights into its catalytic reaction mechanism.
The Journal of biological chemistry 2006;281(40):29807-16.
2006: Takafumi Itoh; Akihito Ochiai; Bunzo Mikami; Wataru Hashimoto; Kousaku Murata
Structure of unsaturated rhamnogalacturonyl hydrolase complexed with substrate.
Biochemical and biophysical research communications 2006;347(4):1021-9.
2006: Akihito Ochiai; Wataru Hashimoto; Kousaku Murata
A biosystem for alginate metabolism in Agrobacterium tumefaciens strain C58: molecular identification of Atu3025 as an exotype family PL-15 alginate lyase.
Research in microbiology 2006;157(7):642-9.
2006: Takafumi Itoh; Akihito Ochiai; Bunzo Mikami; Wataru Hashimoto; Kousaku Murata
A novel glycoside hydrolase family 105: the structure of family 105 unsaturated rhamnogalacturonyl hydrolase complexed with a disaccharide in comparison with family 88 enzyme complexed with the disaccharide.
Journal of molecular biology 2006;360(3):573-85.
2006: Zhongli Cui; Yukie Maruyama; Bunzo Mikami; Wataru Hashimoto; Kousaku Murata
Crystallization and preliminary crystallographic analysis of the family GH78 alpha-L-rhamnosidase RhaB from Bacillus sp. GL1.
Acta crystallographica. Section F, Structural biology and crystallization communications 2006;62(Pt 7):646-8.
2006: Karen Mine Harada; Yuji Aso; Wataru Hashimoto; Bunzo Mikami; Kousaku Murata
Sequence and analysis of the 46.6-kb plasmid pA1 from Sphingomonas sp. A1 that corresponds to the typical IncP-1beta plasmid backbone without any accessory gene.
Plasmid 2006;56(1):11-23.
2006: Takafumi Itoh; Wataru Hashimoto; Bunzo Mikami; Kousaku Murata
Substrate recognition by unsaturated glucuronyl hydrolase from Bacillus sp. GL1.
Biochemical and biophysical research communications 2006;344(1):253-62.
2006: Akihito Ochiai; Masayuki Yamasaki; Bunzo Mikami; Wataru Hashimoto; Kousaku Murata
Crystallization and preliminary X-ray analysis of an exotype alginate lyase Atu3025 from Agrobacterium tumefaciens strain C58, a member of polysaccharide lyase family 15.
Acta crystallographica. Section F, Structural biology and crystallization communications 2006;62(Pt 5):486-8.
2006: Akihito Ochiai; Masayuki Yamasaki; Takafumi Itoh; Bunzo Mikami; Wataru Hashimoto; Kousaku Murata
Crystallization and preliminary X-ray analysis of the rhamnogalacturonan lyase YesW from Bacillus subtilis strain 168, a member of polysaccharide lyase family 11.
Acta crystallographica. Section F, Structural biology and crystallization communications 2006;62(Pt 5):438-40.
2006: Dominggus Malle; Takafumi Itoh; Wataru Hashimoto; Kousaku Murata; Shigeru Utsumi; Bunzo Mikami
Overexpression, purification and preliminary X-ray analysis of pullulanase from Bacillus subtilis strain 168.
Acta crystallographica. Section F, Structural biology and crystallization communications 2006;62(Pt 4):381-4.
2006: Yuji Aso; Yukiko Miyamoto; Karen Mine Harada; Keiko Momma; Shigeyuki Kawai; Wataru Hashimoto; Bunzo Mikami; Kousaku Murata
Engineered membrane superchannel improves bioremediation potential of dioxin-degrading bacteria.
Nature biotechnology 2006;24(2):188-9.
2005: Karen Mine Harada; Keiko Tanaka; Yasuki Fukuda; Wataru Hashimoto; Kousaku Murata
Degradation of rice bran hemicellulose by Paenibacillus sp. strain HC1: gene cloning, characterization and function of beta-D-glucosidase as an enzyme involved in degradation.
Archives of microbiology 2005;184(4):215-24.
2005: Wataru Hashimoto; Jinshan He; Yushin Wada; Hirokazu Nankai; Bunzo Mikami; Kousaku Murata
Proteomics-based identification of outer-membrane proteins responsible for import of macromolecules in Sphingomonas sp. A1: alginate-binding flagellin on the cell surface.
Biochemistry 2005;44(42):13783-94.
2005: Masayuki Yamasaki; Kohei Ogura; Wataru Hashimoto; Bunzo Mikami; Kousaku Murata
A structural basis for depolymerization of alginate by polysaccharide lyase family-7.
Journal of molecular biology 2005;352(1):11-21.
2005: Yukie Maruyama; Wataru Hashimoto; Bunzo Mikami; Kousaku Murata
Crystal structure of Bacillus sp. GL1 xanthan lyase complexed with a substrate: insights into the enzyme reaction mechanism.
Journal of molecular biology 2005;350(5):974-86.
2005: Keiko Momma; Yumiko Mishima; Wataru Hashimoto; Bunzo Mikami; Kousaku Murata
Direct evidence for Sphingomonas sp. A1 periplasmic proteins as macromolecule-binding proteins associated with the ABC transporter: molecular insights into alginate transport in the periplasm.
Biochemistry 2005;44(13):5053-64.
2005: Wataru Hashimoto; Keiko Momma; Yukie Maruyama; Masayuki Yamasaki; Bunzo Mikami; Kousaku Murata
Structure and function of bacterial super-biosystem responsible for import and depolymerization of macromolecules.
Bioscience, biotechnology, and biochemistry 2005;69(4):673-92.
2005: Masayuki Yamasaki; Kohei Ogura; Satoko Moriwaki; Wataru Hashimoto; Kousaku Murata; Bunzo Mikami
Crystallization and preliminary X-ray analysis of alginate lyases A1-II and A1-II' from Sphingomonas sp. A1.
Acta crystallographica. Section F, Structural biology and crystallization communications 2005;61(Pt 3):288-90.
2005: Shigetarou Mori; Masayuki Yamasaki; Yukie Maruyama; Keiko Momma; Shigeyuki Kawai; Wataru Hashimoto; Bunzo Mikami; Kousaku Murata
NAD-binding mode and the significance of intersubunit contact revealed by the crystal structure of Mycobacterium tuberculosis NAD kinase-NAD complex.
Biochemical and biophysical research communications 2005;327(2):500-8.
2005: Wataru Hashimoto; Osamu Miyake; Akihito Ochiai; Kousaku Murata
Molecular identification of Sphingomonas sp. A1 alginate lyase (A1-IV') as a member of novel polysaccharide lyase family 15 and implications in alginate lyase evolution.
Journal of bioscience and bioengineering 2005;99(1):48-54.
2004: Jinshan He; Hirokazu Nankai; Wataru Hashimoto; Kousaku Murata
Molecular identification and characterization of an alginate-binding protein on the cell surface of Sphingomonas sp. A1.
Biochemical and biophysical research communications 2004;322(3):712-7.
2004: Takafumi Itoh; Sae Akao; Wataru Hashimoto; Bunzo Mikami; Kousaku Murata
Crystal structure of unsaturated glucuronyl hydrolase, responsible for the degradation of glycosaminoglycan, from Bacillus sp. GL1 at 1.8 A resolution.
The Journal of biological chemistry 2004;279(30):31804-12.
2004: Masayuki Yamasaki; Satoko Moriwaki; Osamu Miyake; Wataru Hashimoto; Kousaku Murata; Bunzo Mikami
Structure and function of a hypothetical Pseudomonas aeruginosa protein PA1167 classified into family PL-7: a novel alginate lyase with a beta-sandwich fold.
The Journal of biological chemistry 2004;279(30):31863-72.
2004: Osamu Miyake; Akihito Ochiai; Wataru Hashimoto; Kousaku Murata
Origin and diversity of alginate lyases of families PL-5 and -7 in Sphingomonas sp. strain A1.
Journal of bacteriology 2004;186(9):2891-6.
2004: Osamu Miyake; Eiko Kobayashi; Hirokazu Nankai; Wataru Hashimoto; Bunzo Mikami; Kousaku Murata
Posttranslational processing of polysaccharide lyase: maturation route for gellan lyase in Bacillus sp. GL1.
Archives of biochemistry and biophysics 2004;422(2):211-20.
2004: Wataru Hashimoto; Masayuki Yamasaki; Takafumi Itoh; Keiko Momma; Bunzo Mikami; Kousaku Murata
Super-channel in bacteria: structural and functional aspects of a novel biosystem for the import and depolymerization of macromolecules.
Journal of bioscience and bioengineering 2004;98(6):399-413.
2004: Shigetarou Mori; Masayuki Yamasaki; Yukie Maruyama; Keiko Momma; Shigeyuki Kawai; Wataru Hashimoto; Bunzo Mikami; Kousaku Murata
Crystallographic studies of Mycobacterium tuberculosis polyphosphate/ATP-NAD kinase complexed with NAD.
Journal of bioscience and bioengineering 2004;98(5):391-3.
2003: Masayuki Yamasaki; Satoko Moriwaki; Wataru Hashimoto; Bunzo Mikami; Kousaku Murata
Crystallization and preliminary X-ray analysis of alginate lyase, a member of family PL-7, from Pseudomonas aeruginosa.
Acta crystallographica. Section D, Biological crystallography 2003;59(Pt 8):1499-501.
2003: Wataru Hashimoto; Osamu Miyake; Hirokazu Nankai; Kousaku Murata
Molecular identification of an alpha-L-rhamnosidase from Bacillus sp strain GL1 as an enzyme involved in complete metabolism of gellan.
Archives of biochemistry and biophysics 2003;415(2):235-44.
2003: Shigetarou Mori; Sae Akao; Osamu Miyake; Hirokazu Nankai; Wataru Hashimoto; Bunzo Mikami; Kousaku Murata
Crystallization and preliminary X-ray analysis of a novel unsaturated glucuronyl hydrolase from Bacillus sp. GL1.
Acta crystallographica. Section D, Biological crystallography 2003;59(Pt 5):946-9.
2003: Shigetarou Mori; Sae Akao; Hirokazu Nankai; Wataru Hashimoto; Bunzo Mikami; Kousaku Murata
A novel member of glycoside hydrolase family 88: overexpression, purification, and characterization of unsaturated beta-glucuronyl hydrolase of Bacillus sp. GL1.
Protein expression and purification 2003;29(1):77-84.
2003: Osamu Miyake; Wataru Hashimoto; Kousaku Murata
An exotype alginate lyase in Sphingomonas sp. A1: overexpression in Escherichia coli, purification, and characterization of alginate lyase IV (A1-IV).
Protein expression and purification 2003;29(1):33-41.
2003: Wataru Hashimoto; Hirokazu Nankai; Bunzo Mikami; Kousaku Murata
Crystal structure of Bacillus sp. GL1 xanthan lyase, which acts on the side chains of xanthan.
The Journal of biological chemistry 2003;278(9):7663-73.
2003: Yumiko Mishima; Keiko Momma; Wataru Hashimoto; Bunzo Mikami; Kousaku Murata
Crystal structure of AlgQ2, a macromolecule (alginate)-binding protein of Sphingomonas sp. A1, complexed with an alginate tetrasaccharide at 1.6-A resolution.
The Journal of biological chemistry 2003;278(8):6552-9.
2002: Wataru Hashimoto; Bunzo Mikami; Kousaku Murata
[Structure and function of bacterial "super-channel" for macromolecule import].
Seikagaku. The Journal of Japanese Biochemical Society 2002;74(7):563-7.
2002: Hirokazu Nankai; Wataru Hashimoto; Kousaku Murata
Molecular identification of family 38 alpha-mannosidase of Bacillus sp. strain GL1, responsible for complete depolymerization of xanthan.
Applied and environmental microbiology 2002;68(6):2731-6.
2002: Keiko Momma; Bunzo Mikami; Yumiko Mishima; Wataru Hashimoto; Kousaku Murata
Crystal structure of AlgQ2, a macromolecule (alginate)-binding protein of Sphingomonas sp. A1 at 2.0A resolution.
Journal of molecular biology 2002;316(5):1051-9.
2002: Yoshiyuki Hayama; Yasuki Fukuda; Shigeyuki Kawai; Wataru Hashimoto; Kousaku Murata
Extremely simple, rapid and highly efficient transformation method for the yeast Saccharomyces cerevisiae using glutathione and early log phase cells.
Journal of bioscience and bioengineering 2002;94(2):166-71.
2002: Yumiko Mishima; Keiko Momma; Osamu Miyake; Wataru Hashimoto; Bunzo Mikami; Kousaku Murata
A super-channel in bacteria: macromolecule uptake and depolymerization systems of Sphingomonas sp. A1 with a special cell surface structure.
Biotechnology & genetic engineering reviews 2002;19():105-19.
2001: Yumiko Mishima; Keiko Momma; Wataru Hashimoto; Bunzo Mikami; Kousaku Murata
Super-channel in bacteria: function and structure of the macromolecule import system mediated by a pit-dependent ABC transporter.
FEMS microbiology letters 2001;204(2):215-21.
2001: Wataru Hashimoto; Keiko Momma; Yumiko Mishima; Bunzo Mikami; Kousaku Murata
Super-channel in bacteria: function and structure of a macromolecule import system mediated by a pit-dependent ABC transporter.
Bioscience, biotechnology, and biochemistry 2001;65(9):1949-56.
2001: Shigeyuki Kawai; Shigetarou Mori; Takako Mukai; Wataru Hashimoto; Kousaku Murata
Molecular characterization of Escherichia coli NAD kinase.
European journal of biochemistry / FEBS 2001;268(15):4359-65.
2001: Yumiko Mishima; Keiko Momma; Wataru Hashimoto; Bunzo Mikami; Kousaku Murata
Crystallization and preliminary X-ray analysis of AlgS, a bacterial ATP-binding cassette (ABC) protein specific to macromolecule import.
Acta crystallographica. Section D, Biological crystallography 2001;57(Pt 6):884-5.
2001: Hye-Jin Yoon; Wataru Hashimoto; Osamu Miyake; Kousaku Murata; Bunzo Mikami
Crystal structure of alginate lyase A1-III complexed with trisaccharide product at 2.0 A resolution.
Journal of molecular biology 2001;307(1):9-16.
2001: Wataru Hashimoto; H Miki; N Tsuchiya; Hirokazu Nankai; Kousaku Murata
Polysaccharide lyase: molecular cloning, sequencing, and overexpression of the xanthan lyase gene of Bacillus sp. strain GL1.
Applied and environmental microbiology 2001;67(2):713-20.
2000: Shigeyuki Kawai; Shigetarou Mori; Takako Mukai; Sachiko Suzuki; T Yamada; Wataru Hashimoto; Kousaku Murata
Inorganic Polyphosphate/ATP-NAD kinase of Micrococcus flavus and Mycobacterium tuberculosis H37Rv.
Biochemical and biophysical research communications 2000;276(1):57-63.
2000: Keiko Momma; Wataru Hashimoto; H J Yoon; S Ozawa; Y Fukuda; Shigeyuki Kawai; F Takaiwa; Shigeru Utsumi; Kousaku Murata
Safety assessment of rice genetically modified with soybean glycinin by feeding studies on rats.
Bioscience, biotechnology, and biochemistry 2000;64(9):1881-6.
2000: Wataru Hashimoto; Osamu Miyake; Keiko Momma; Shigeyuki Kawai; Kousaku Murata
Molecular identification of oligoalginate lyase of Sphingomonas sp. strain A1 as one of the enzymes required for complete depolymerization of alginate.
Journal of bacteriology 2000;182(16):4572-7.
2000: Keiko Momma; M Okamoto; Yumiko Mishima; Shigetarou Mori; Wataru Hashimoto; Kousaku Murata
A novel bacterial ATP-binding cassette transporter system that allows uptake of macromolecules.
Journal of bacteriology 2000;182(14):3998-4004.
2000: Hye-Jin Yoon; Wataru Hashimoto; Osamu Miyake; M Okamoto; Bunzo Mikami; Kousaku Murata
Overexpression in Escherichia coli, purification, and characterization of Sphingomonas sp. A1 alginate lyases.
Protein expression and purification 2000;19(1):84-90.
2000: Hye-Jin Yoon; Wataru Hashimoto; Yoshio Katsuya; Yoshihiro Mezaki; Kousaku Murata; Bunzo Mikami
Crystallization and preliminary X-ray crystallographic analysis of alginate lyase A1-II from Sphingomonas species A1.
Biochimica et biophysica acta 2000;1476(2):382-5.
1999: Wataru Hashimoto; Keiko Momma; H J Yoon; S Ozawa; Y Ohkawa; Takeru Ishige; Makoto Kito; Shigeru Utsumi; Kousaku Murata
Safety assessment of transgenic potatoes with soybean glycinin by feeding studies in rats.
Bioscience, biotechnology, and biochemistry 1999;63(11):1942-6.
1999: Wataru Hashimoto; Eiko Kobayashi; Hirokazu Nankai; N Sato; T Miya; Shigeyuki Kawai; Kousaku Murata
Unsaturated glucuronyl hydrolase of Bacillus sp. GL1: novel enzyme prerequisite for metabolism of unsaturated oligosaccharides produced by polysaccharide lyases.
Archives of biochemistry and biophysics 1999;368(2):367-74.
1999: Wataru Hashimoto; Hirokazu Nankai; N Sato; Shigeyuki Kawai; Kousaku Murata
Characterization of alpha-L-rhamnosidase of Bacillus sp. GL1 responsible for the complete depolymerization of gellan.
Archives of biochemistry and biophysics 1999;368(1):56-60.
1999: Hye-Jin Yoon; Bunzo Mikami; Wataru Hashimoto; Kousaku Murata
Crystal structure of alginate lyase A1-III from Sphingomonas species A1 at 1.78 A resolution.
Journal of molecular biology 1999;290(2):505-14.
1999: Hirokazu Nankai; Wataru Hashimoto; H Miki; Shigeyuki Kawai; Kousaku Murata
Microbial system for polysaccharide depolymerization: enzymatic route for xanthan depolymerization by Bacillus sp. strain GL1.
Applied and environmental microbiology 1999;65(6):2520-6.
1999: Keiko Momma; Wataru Hashimoto; S Ozawa; Shigeyuki Kawai; T Katsube; F Takaiwa; Makoto Kito; Shigeru Utsumi; Kousaku Murata
Quality and safety evaluation of genetically engineered rice with soybean glycinin: analyses of the grain composition and digestibility of glycinin in transgenic rice.
Bioscience, biotechnology, and biochemistry 1999;63(2):314-8.
1999: Wataru Hashimoto; Keiko Momma; H Miki; Yumiko Mishima; Eiko Kobayashi; Osamu Miyake; Shigeyuki Kawai; Hirokazu Nankai; Bunzo Mikami; Kousaku Murata
Enzymatic and genetic bases on assimilation, depolymerization, and transport of heteropolysaccharides in bacteria.
Journal of bioscience and bioengineering 1999;87(2):123-36.
1998: Wataru Hashimoto; H Miki; Hirokazu Nankai; N Sato; Shigeyuki Kawai; Kousaku Murata
Molecular cloning of two genes for beta-D-glucosidase in Bacillus sp. GL1 and identification of one as a gellan-degrading enzyme.
Archives of biochemistry and biophysics 1998;360(1):1-9.
1998: Wataru Hashimoto; H Miki; N Tsuchiya; Hirokazu Nankai; Kousaku Murata
Xanthan lyase of Bacillus sp. strain GL1 liberates pyruvylated mannose from xanthan side chains.
Applied and environmental microbiology 1998;64(10):3765-8.
1998: Nobuaki Shirai; Keiko Momma; S Ozawa; Wataru Hashimoto; Makoto Kito; Shigeru Utsumi; Kousaku Murata
Safety assessment of genetically engineered food: detection and monitoring of glyphosate-tolerant soybeans.
Bioscience, biotechnology, and biochemistry 1998;62(7):1461-4.
1998: Wataru Hashimoto; Kousaku Murata
alpha-L-rhamnosidase of Sphingomonas sp. R1 producing an unusual exopolysaccharide of sphingan.
Bioscience, biotechnology, and biochemistry 1998;62(6):1068-74.
1998: Wataru Hashimoto; N Sato; S Kimura; Kousaku Murata
Polysaccharide lyase: molecular cloning of gellan lyase gene and formation of the lyase from a huge precursor protein in Bacillus sp. GL1.
Archives of biochemistry and biophysics 1998;354(1):31-9.
1997: Wataru Hashimoto; K Maesaka; N Sato; S Kimura; Kotaro T Yamamoto; Hidehiko Kumagai; Kousaku Murata
Microbial system for polysaccharide depolymerization: enzymatic route for gellan depolymerization by Bacillus sp. GL1.
Archives of biochemistry and biophysics 1997;339(1):17-23.
1997: Wataru Hashimoto; Hideyuki Suzuki; Kotaro T Yamamoto; Hidehiko Kumagai
Analysis of low temperature inducible mechanism of gamma-glutamyltranspeptidase of Escherichia coli K-12.
Bioscience, biotechnology, and biochemistry 1997;61(1):34-9.
1996: I Maru; J Ohnishi; Y Ohta; Wataru Hashimoto; Y Tsukada; K Murata; Bunzo Mikami
Crystallization and preliminary X-ray diffraction studies of N-acyl-D-glucosamine 2-epimerase from porcine kidney.
Journal of biochemistry 1996;120(3):481-2.
1996: H Sakai; N Sakabe; K Sasaki; Wataru Hashimoto; Hideyuki Suzuki; H Tachi; Hidehiko Kumagai; K Sakabe
A preliminary description of the crystal structure of gamma-glutamyltranspeptidase from E. coli K-12.
Journal of biochemistry 1996;120(1):26-8.
1995: Wataru Hashimoto; Hideyuki Suzuki; S Nohara; H Tachi; K Yamamoto; Hidehiko Kumagai
Subunit association of gamma-glutamyltranspeptidase of Escherichia coli K-12.
Journal of biochemistry 1995;118(6):1216-23.
1995: Wataru Hashimoto; Hideyuki Suzuki; K Yamamoto; Hidehiko Kumagai
Effect of site-directed mutations on processing and activity of gamma-glutamyltranspeptidase of Escherichia coli K-12.
Journal of biochemistry 1995;118(1):75-80.
1993: Hidehiko Kumagai; S Nohara; Hideyuki Suzuki; Wataru Hashimoto; K Yamamoto; H Sakai; K Sakabe; K Fukuyama; N Sakabe
Crystallization and preliminary X-ray analysis of gamma-glutamyltranspeptidase from Escherichia coli K-12.
Journal of molecular biology 1993;234(4):1259-62.
1993: Hideyuki Suzuki; Wataru Hashimoto; Hidehiko Kumagai
Escherichia coli K-12 can utilize an exogenous gamma-glutamyl peptide as an amino acid source, for which gamma-glutamyltranspeptidase is essential.
Journal of bacteriology 1993;175(18):6038-40.
1992: Wataru Hashimoto; Hideyuki Suzuki; S Nohara; Hidehiko Kumagai
Escherichia coli gamma-glutamyltranspeptidase mutants deficient in processing to subunits.
Biochemical and biophysical research communications 1992;189(1):173-8.