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Robert Hausinger
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11
Mulrooney, Scott
10
Ryle, Matthew
8
Karplus, Andrew
6
Grzyska, Piotr
6
Que, Lawrence
5
Pearson, MA
4
Scott, Robert
4
Liu, Aimin
4
McCracken, John
3
Michel, LO
3
Koehntop, Kevin
3
Colpas, GJ
3
Dunning Hotopp, Julie
3
Feig, Michael
3
Henshaw, Timothy
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All Publications
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2008: Quiroz-Valenzuela Soledad; Sukuru Sai Chetan K; Hausinger Robert P; Kuhn Leslie A; Heller William T
The structure of urease activation complexes examined by flexibility analysis, mutagenesis, and small-angle X-ray scattering.
Archives of biochemistry and biophysics 2008;480(1):51-7.
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2008: Simmons Jana M; Müller Tina A; Hausinger Robert P
Fe(II)/alpha-ketoglutarate hydroxylases involved in nucleobase, nucleoside, nucleotide, and chromatin metabolism.
Dalton transactions (Cambridge, England : 2003) 2008;(38):5132-42.
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2008: Kalliri Efthalia; Mulrooney Scott B; Hausinger Robert P
Identification of Escherichia coli YgaF as an L-2-hydroxyglutarate oxidase.
Journal of bacteriology 2008;190(11):3793-8.
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2008: Li Meng; Müller Tina A; Fraser Bruce A; Hausinger Robert P
Characterization of active site variants of xanthine hydroxylase from Aspergillus nidulans.
Archives of biochemistry and biophysics 2008;470(1):44-53.
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2007: Grzyska Piotr K; Hausinger Robert P
Cr(II) reactivity of taurine/alpha-ketoglutarate dioxygenase.
Inorganic chemistry 2007;46(24):10087-92.
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2007: Grossoehme Nicholas E; Mulrooney Scott B; Hausinger Robert P; Wilcox Dean E
Thermodynamics of Ni2+, Cu2+, and Zn2+ binding to the urease metallochaperone UreE.
Biochemistry 2007;46(37):10506-16.
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2007: Muthukumaran Rajendra Bose; Grzyska Piotr K; Hausinger Robert P; McCracken John
Probing the iron-substrate orientation for taurine/alpha-ketoglutarate dioxygenase using deuterium electron spin echo envelope modulation spectroscopy.
Biochemistry 2007;46(20):5951-9.
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2007: Montero-Morán Gabriela M; Li Meng; Rendòn-Huerta Erika; Jourdan Fabrice; Lowe David J; Stumpff-Kane Andrew W; Feig Michael; Scazzocchio Claudio; Hausinger Robert P
Purification and characterization of the FeII- and alpha-ketoglutarate-dependent xanthine hydroxylase from Aspergillus nidulans.
Biochemistry 2007;46(18):5293-304.
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2007: Grzyska Piotr K; Müller Tina A; Campbell Melody G; Hausinger Robert P
Metal ligand substitution and evidence for quinone formation in taurine/alpha-ketoglutarate dioxygenase.
Journal of inorganic biochemistry 2007;101(5):797-808.
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2007: Hausinger Robert P
New insights into acetone metabolism.
Journal of bacteriology 2007;189(3):671-3.
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2007: Yu Zhong; Genest Paul-André; ter Riet Bas; Sweeney Kate; DiPaolo Courtney; Kieft Rudo; Christodoulou Evangelos; Perrakis Anastassis; Simmons Jana M; Hausinger Robert P; van Luenen Henri G A M; Rigden Daniel J; Sabatini Robert; Borst Piet
The protein that binds to DNA base J in trypanosomatids has features of a thymidine hydroxylase.
Nucleic acids research 2007;35(7):2107-15.
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2006: Kim Jong Kyong; Mulrooney Scott B; Hausinger Robert P
The UreEF fusion protein provides a soluble and functional form of the UreF urease accessory protein.
Journal of bacteriology 2006;188(24):8413-20.
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2006: Zhang Li; Mulrooney Scott B; Leung Andy F K; Zeng Yibo; Ko Ben B C; Hausinger Robert P; Sun Hongzhe
Inhibition of urease by bismuth(III): implications for the mechanism of action of bismuth drugs.
Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine 2006;19(5):503-11.
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2006: Müller Tina A; Zavodszky Maria I; Feig Michael; Kuhn Leslie A; Hausinger Robert P
Structural basis for the enantiospecificities of R- and S-specific phenoxypropionate/alpha-ketoglutarate dioxygenases.
Protein science : a publication of the Protein Society 2006;15(6):1356-68.
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2006: Luo Lusong; Pappalardi Melissa B; Tummino Peter J; Copeland Robert A; Fraser Marie E; Grzyska Piotr K; Hausinger Robert P
An assay for Fe(II)/2-oxoglutarate-dependent dioxygenases by enzyme-coupled detection of succinate formation.
Analytical biochemistry 2006;353(1):69-74.
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2006: Rohankhedkar Mukta S; Mulrooney Scott B; Wedemeyer William J; Hausinger Robert P
The AidB component of the Escherichia coli adaptive response to alkylating agents is a flavin-containing, DNA-binding protein.
Journal of bacteriology 2006;188(1):223-30.
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2006: Koehntop Kevin D; Marimanikkuppam Sudha; Ryle Matthew J; Hausinger Robert P; Que Lawrence
Self-hydroxylation of taurine/alpha-ketoglutarate dioxygenase: evidence for more than one oxygen activation mechanism.
Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry 2006;11(1):63-72.
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2005: Kalliri Efthalia; Grzyska Piotr K; Hausinger Robert P
Kinetic and spectroscopic investigation of CoII, NiII, and N-oxalylglycine inhibition of the FeII/alpha-ketoglutarate dioxygenase, TauD.
Biochemical and biophysical research communications 2005;338(1):191-7.
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2005: Kim Jong Kyong; Mulrooney Scott B; Hausinger Robert P
Biosynthesis of active Bacillus subtilis urease in the absence of known urease accessory proteins.
Journal of bacteriology 2005;187(20):7150-4.
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2005: Mulrooney Scott B; Ward Sarah K; Hausinger Robert P
Purification and properties of the Klebsiella aerogenes UreE metal-binding domain, a functional metallochaperone of urease.
Journal of bacteriology 2005;187(10):3581-5.
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2005: Grzyska Piotr K; Ryle Matthew J; Monterosso Greta R; Liu Jian; Ballou David P; Hausinger Robert P
Steady-state and transient kinetic analyses of taurine/alpha-ketoglutarate dioxygenase: effects of oxygen concentration, alternative sulfonates, and active-site variants on the FeIV-oxo intermediate.
Biochemistry 2005;44(10):3845-55.
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2004: Henshaw Timothy F; Feig Michael; Hausinger Robert P
Aberrant activity of the DNA repair enzyme AlkB.
Journal of inorganic biochemistry 2004;98(5):856-61.
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2004: Hausinger Robert P
Metabolic versatility of prokaryotes for urea decomposition.
Journal of bacteriology 2004;186(9):2520-2.
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2004: Chang Zhenzhan; Kuchar Jason; Hausinger Robert P
Chemical cross-linking and mass spectrometric identification of sites of interaction for UreD, UreF, and urease.
The Journal of biological chemistry 2004;279(15):15305-13.
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2004: Kuchar Jason; Hausinger Robert P
Biosynthesis of metal sites.
Chemical reviews 2004;104(2):509-25.
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2004: Proshlyakov Denis A; Henshaw Timothy F; Monterosso Greta R; Ryle Matthew J; Hausinger Robert P
Direct detection of oxygen intermediates in the non-heme Fe enzyme taurine/alpha-ketoglutarate dioxygenase.
Journal of the American Chemical Society 2004;126(4):1022-3.
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2004: Hausinger Robert P
FeII/alpha-ketoglutarate-dependent hydroxylases and related enzymes.
Critical reviews in biochemistry and molecular biology 2004;39(1):21-68.
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2003: Mulrooney Scott B; Hausinger Robert P
Nickel uptake and utilization by microorganisms.
FEMS microbiology reviews 2003;27(2-3):239-61.
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2003: Hausinger Robert P
Ni and CO: more surprises.
Nature structural biology 2003;10(4):234-6.
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2003: Ryle Matthew J; Koehntop Kevin D; Liu Aimin; Que Lawrence; Hausinger Robert P
Interconversion of two oxidized forms of taurine/alpha-ketoglutarate dioxygenase, a non-heme iron hydroxylase: evidence for bicarbonate binding.
Proceedings of the National Academy of Sciences of the United States of America 2003;100(7):3790-5.
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2003: Ryle Matthew J; Liu Aimin; Muthukumaran Rajendra Bose; Ho Raymond Y N; Koehntop Kevin D; McCracken John; Que Lawrence; Hausinger Robert P
O2- and alpha-ketoglutarate-dependent tyrosyl radical formation in TauD, an alpha-keto acid-dependent non-heme iron dioxygenase.
Biochemistry 2003;42(7):1854-62.
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2003: Dunning Hotopp Julie C; Auchtung Thomas A; Hogan Deborah A; Hausinger Robert P
Intrinsic tryptophan fluorescence as a probe of metal and alpha-ketoglutarate binding to TfdA, a mononuclear non-heme iron dioxygenase.
Journal of inorganic biochemistry 2003;93(1-2):66-70.
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2003: Mulrooney Scott B; Hausinger Robert P
Metal ion dependence of recombinant Escherichia coli allantoinase.
Journal of bacteriology 2003;185(1):126-34.
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2002: Trewick Sarah C; Henshaw Timothy F; Hausinger Robert P; Lindahl Tomas; Sedgwick Barbara
Oxidative demethylation by Escherichia coli AlkB directly reverts DNA base damage.
Nature 2002;419(6903):174-8.
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2002: Dunning Hotopp Julie C; Hausinger Robert P
Probing the 2,4-dichlorophenoxyacetate/alpha-ketoglutarate dioxygenase substrate-binding site by site-directed mutagenesis and mechanism-based inactivation.
Biochemistry 2002;41(31):9787-94.
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2002: Itoh Kazuhito; Kanda Rie; Sumita Yoko; Kim Hongik; Kamagata Yoichi; Suyama Kousuke; Yamamoto Hiroki; Hausinger Robert P; Tiedje James M
tfdA-like genes in 2,4-dichlorophenoxyacetic acid-degrading bacteria belonging to the Bradyrhizobium-Agromonas-Nitrobacter-Afipia cluster in alpha-Proteobacteria.
Applied and environmental microbiology 2002;68(7):3449-54.
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2002: Ryle Matthew J; Hausinger Robert P
Non-heme iron oxygenases.
Current opinion in chemical biology 2002;6(2):193-201.
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2002: Elkins Jonathan M; Ryle Matthew J; Clifton Ian J; Dunning Hotopp Julie C; Lloyd John S; Burzlaff Nicolai I; Baldwin Jack E; Hausinger Robert P; Roach Peter L
X-ray crystal structure of Escherichia coli taurine/alpha-ketoglutarate dioxygenase complexed to ferrous iron and substrates.
Biochemistry 2002;41(16):5185-92.
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2001: Song H K; Mulrooney S B; Huber R; Hausinger R P
Crystal structure of Klebsiella aerogenes UreE, a nickel-binding metallochaperone for urease activation.
The Journal of biological chemistry 2001;276(52):49359-64.
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2001: Mulrooney S; Zakharian T; Schaller R A; Hausinger R P
Dual effects of ionic strength on Klebsiella aerogenes urease: pH-dependent activation and inhibition.
Archives of biochemistry and biophysics 2001;394(2):280-2.
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2001: Liu A; Ho R Y; Que L; Ryle M J; Phinney B S; Hausinger R P
Alternative reactivity of an alpha-ketoglutarate-dependent iron(II) oxygenase: enzyme self-hydroxylation.
Journal of the American Chemical Society 2001;123(21):5126-7.
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2001: Ho R Y; Mehn M P; Hegg E L; Liu A; Ryle M J; Hausinger R P; Que L
Resonance Raman studies of the iron(II)--alpha-keto acid chromophore in model and enzyme complexes.
Journal of the American Chemical Society 2001;123(21):5022-9.
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2000: Soriano A; Colpas G J; Hausinger R P
UreE stimulation of GTP-dependent urease activation in the UreD-UreF-UreG-urease apoprotein complex.
Biochemistry 2000;39(40):12435-40.
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2000: Pearson M A; Park I S; Schaller R A; Michel L O; Karplus P A; Hausinger R P
Kinetic and structural characterization of urease active site variants.
Biochemistry 2000;39(29):8575-84.
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2000: Todd M J; Hausinger R P
Fluoride inhibition of Klebsiella aerogenes urease: mechanistic implications of a pseudo-uncompetitive, slow-binding inhibitor.
Biochemistry 2000;39(18):5389-96.
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2000: Hogan D A; Smith S R; Saari E A; McCracken J; Hausinger R P
Site-directed mutagenesis of 2,4-dichlorophenoxyacetic acid/alpha-ketoglutarate dioxygenase. Identification of residues involved in metallocenter formation and substrate binding.
The Journal of biological chemistry 2000;275(17):12400-9.
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2000: Colpas G J; Hausinger R P
In vivo and in vitro kinetics of metal transfer by the Klebsiella aerogenes urease nickel metallochaperone, UreE.
The Journal of biological chemistry 2000;275(15):10731-7.
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1999: Hegg E L; Whiting A K; Saari R E; McCracken J; Hausinger R P; Que L
Herbicide-degrading alpha-keto acid-dependent enzyme TfdA: metal coordination environment and mechanistic insights.
Biochemistry 1999;38(50):16714-26.
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1999: Ryle M J; Padmakumar R; Hausinger R P
Stopped-flow kinetic analysis of Escherichia coli taurine/alpha-ketoglutarate dioxygenase: interactions with alpha-ketoglutarate, taurine, and oxygen.
Biochemistry 1999;38(46):15278-86.
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1999: Soriano A; Hausinger R P
GTP-dependent activation of urease apoprotein in complex with the UreD, UreF, and UreG accessory proteins.
Proceedings of the National Academy of Sciences of the United States of America 1999;96(20):11140-4.
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1999: Hogan D A; Auchtung T A; Hausinger R P
Cloning and characterization of a sulfonate/alpha-ketoglutarate dioxygenase from Saccharomyces cerevisiae.
Journal of bacteriology 1999;181(18):5876-9.
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1999: Yamaguchi K; Cosper N J; Stålhandske C; Scott R A; Pearson M A; Karplus P A; Hausinger R P
Characterization of metal-substituted Klebsiella aerogenes urease.
Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry 1999;4(4):468-77.
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1999: Colpas G J; Brayman T G; Ming L J; Hausinger R P
Identification of metal-binding residues in the Klebsiella aerogenes urease nickel metallochaperone, UreE.
Biochemistry 1999;38(13):4078-88.
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1999: Romanov V; Merski M T; Hausinger R P
Assays for allantoinase.
Analytical biochemistry 1999;268(1):49-53.
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1999: Cosper N J; Stålhandske C M; Saari R E; Hausinger R P; Scott R A
X-ray absorption spectroscopic analysis of Fe(II) and Cu(II) forms of a herbicide-degrading alpha-ketoglutarate dioxygenase.
Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry 1999;4(1):122-9.
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1998: Pearson M A; Schaller R A; Michel L O; Karplus P A; Hausinger R P
Chemical rescue of Klebsiella aerogenes urease variants lacking the carbamylated-lysine nickel ligand.
Biochemistry 1998;37(17):6214-20.
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1998: Saari R E; Hausinger R P
Ascorbic acid-dependent turnover and reactivation of 2,4-dichlorophenoxyacetic acid/alpha-ketoglutarate dioxygenase using thiophenoxyacetic acid.
Biochemistry 1998;37(9):3035-42.
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1997: Yamaguchi K; Hausinger R P
Substitution of the urease active site carbamate by dithiocarbamate and vanadate.
Biochemistry 1997;36(49):15118-22.
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1997: Moncrief M B; Hausinger R P
Characterization of UreG, identification of a UreD-UreF-UreG complex, and evidence suggesting that a nucleotide-binding site in UreG is required for in vivo metallocenter assembly of Klebsiella aerogenes urease.
Journal of bacteriology 1997;179(13):4081-6.
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1997: Pearson M A; Michel L O; Hausinger R P; Karplus P A
Structures of Cys319 variants and acetohydroxamate-inhibited Klebsiella aerogenes urease.
Biochemistry 1997;36(26):8164-72.
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1996: Moncrief M B; Hausinger R P
Purification and activation properties of UreD-UreF-urease apoprotein complexes.
Journal of bacteriology 1996;178(18):5417-21.
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1996: Brayman T G; Hausinger R P
Purification, characterization, and functional analysis of a truncated Klebsiella aerogenes UreE urease accessory protein lacking the histidine-rich carboxyl terminus.
Journal of bacteriology 1996;178(18):5410-6.
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1996: Park I S; Michel L O; Pearson M A; Jabri E; Karplus P A; Wang S; Dong J; Scott R A; Koehler B P; Johnson M K; Hausinger R P
Characterization of the mononickel metallocenter in H134A mutant urease.
The Journal of biological chemistry 1996;271(31):18632-7.
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1996: Suwa Y; Wright A D; Fukimori F; Nummy K A; Hausinger R P; Holben W E; Forney L J
Characterization of a chromosomally encoded 2,4-dichlorophenoxyacetic acid/alpha-ketoglutarate dioxygenase from Burkholderia sp. strain RASC.
Applied and environmental microbiology 1996;62(7):2464-9.
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1996: Romanov V; Hausinger R P
NADPH-dependent reductive ortho dehalogenation of 2,4-dichlorobenzoic acid in Corynebacterium sepedonicum KZ-4 and Coryneform bacterium strainNTB-1 via 2,4-dichlorobenzoyl coenzyme A.
Journal of bacteriology 1996;178(9):2656-61.
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1996: Park I S; Hausinger R P
Metal ion interaction with urease and UreD-urease apoproteins.
Biochemistry 1996;35(16):5345-52.
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1995: Moncrief M B; Hom L G; Jabri E; Karplus P A; Hausinger R P
Urease activity in the crystalline state.
Protein science : a publication of the Protein Society 1995;4(10):2234-6.
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1995: Hausinger R P; Fukumori F
Characterization of the first enzyme in 2,4-dichlorophenoxyacetic acid metabolism.
Environmental health perspectives 1995;103 Suppl 5():37-9.
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1995: Jabri E; Carr M B; Hausinger R P; Karplus P A
The crystal structure of urease from Klebsiella aerogenes.
Science (New York, N.Y.) 1995;268(5213):998-1004.
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1995: Park I S; Hausinger R P
Evidence for the presence of urease apoprotein complexes containing UreD, UreF, and UreG in cells that are competent for in vivo enzyme activation.
Journal of bacteriology 1995;177(8):1947-51.
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1995: Park I S; Hausinger R P
Requirement of carbon dioxide for in vitro assembly of the urease nickel metallocenter.
Science (New York, N.Y.) 1995;267(5201):1156-8.
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1994: Romanov V; Hausinger R P
Pseudomonas aeruginosa 142 uses a three-component ortho-halobenzoate 1,2-dioxygenase for metabolism of 2,4-dichloro- and 2-chlorobenzoate.
Journal of bacteriology 1994;176(11):3368-74.
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1994: Hausinger R P
Nickel enzymes in microbes.
The Science of the total environment 1994;148(2-3):157-66.
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1994: Park I S; Carr M B; Hausinger R P
In vitro activation of urease apoprotein and role of UreD as a chaperone required for nickel metallocenter assembly.
Proceedings of the National Academy of Sciences of the United States of America 1994;91(8):3233-7.
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1993: Fukumori F; Hausinger R P
Purification and characterization of 2,4-dichlorophenoxyacetate/alpha-ketoglutarate dioxygenase.
The Journal of biological chemistry 1993;268(32):24311-7.
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1993: Lee M H; Pankratz H S; Wang S; Scott R A; Finnegan M G; Johnson M K; Ippolito J A; Christianson D W; Hausinger R P
Purification and characterization of Klebsiella aerogenes UreE protein: a nickel-binding protein that functions in urease metallocenter assembly.
Protein science : a publication of the Protein Society 1993;2(6):1042-52.
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1993: Park I S; Hausinger R P
Site-directed mutagenesis of Klebsiella aerogenes urease: identification of histidine residues that appear to function in nickel ligation, substrate binding, and catalysis.
Protein science : a publication of the Protein Society 1993;2(6):1034-41.
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1993: Fukumori F; Hausinger R P
Alcaligenes eutrophus JMP134 "2,4-dichlorophenoxyacetate monooxygenase" is an alpha-ketoglutarate-dependent dioxygenase.
Journal of bacteriology 1993;175(7):2083-6.
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1993: Park I S; Hausinger R P
Diethylpyrocarbonate reactivity of Klebsiella aerogenes urease: effect of pH and active site ligands on the rate of inactivation.
Journal of protein chemistry 1993;12(1):51-6.
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1992: Jabri E; Lee M H; Hausinger R P; Karplus P A
Preliminary crystallographic studies of urease from jack bean and from Klebsiella aerogenes.
Journal of molecular biology 1992;227(3):934-7.
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1992: Martin P R; Hausinger R P
Site-directed mutagenesis of the active site cysteine in Klebsiella aerogenes urease.
The Journal of biological chemistry 1992;267(28):20024-7.
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1992: Lee M H; Mulrooney S B; Renner M J; Markowicz Y; Hausinger R P
Klebsiella aerogenes urease gene cluster: sequence of ureD and demonstration that four accessory genes (ureD, ureE, ureF, and ureG) are involved in nickel metallocenter biosynthesis.
Journal of bacteriology 1992;174(13):4324-30.
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1992: McCoy D D; Cetin A; Hausinger R P
Characterization of urease from Sporosarcina ureae.
Archives of microbiology 1992;157(5):411-6.
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1991: Todd M J; Hausinger R P
Identification of the essential cysteine residue in Klebsiella aerogenes urease.
The Journal of biological chemistry 1991;266(36):24327-31.
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1991: Todd M J; Hausinger R P
Reactivity of the essential thiol of Klebsiella aerogenes urease. Effect of pH and ligands on thiol modification.
The Journal of biological chemistry 1991;266(16):10260-7.
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1990: Mulrooney S B; Hausinger R P
Sequence of the Klebsiella aerogenes urease genes and evidence for accessory proteins facilitating nickel incorporation.
Journal of bacteriology 1990;172(10):5837-43.
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1990: Lee M H; Mulrooney S B; Hausinger R P
Purification, characterization, and in vivo reconstitution of Klebsiella aerogenes urease apoenzyme.
Journal of bacteriology 1990;172(8):4427-31.
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1990: Hausinger R P
Mechanisms of metal ion incorporation into metalloproteins.
BioFactors (Oxford, England) 1990;2(3):179-84.
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1989: Todd M J; Hausinger R P
Competitive inhibitors of Klebsiella aerogenes urease. Mechanisms of interaction with the nickel active site.
The Journal of biological chemistry 1989;264(27):15835-42.
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1989: Mulrooney S B; Pankratz H S; Hausinger R P
Regulation of gene expression and cellular localization of cloned Klebsiella aerogenes (K. pneumoniae) urease.
Journal of general microbiology 1989;135(6):1769-76.
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1988: Gloss L M; Hausinger R P
Methanogen factor 390 formation: species distribution, reversibility and effects of non-oxidative cellular stresses.
BioFactors (Oxford, England) 1988;1(3):237-40.
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1988: Mulrooney S B; Lynch M J; Mobley H L; Hausinger R P
Purification, characterization, and genetic organization of recombinant Providencia stuartii urease expressed by Escherichia coli.
Journal of bacteriology 1988;170(5):2202-7.
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1988: Simpson D A; Hausinger R P; Mulks M H
Purification, characterization, and comparison of the immunoglobulin A1 proteases of Neisseria gonorrhoeae.
Journal of bacteriology 1988;170(4):1866-73.
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1988: Breitenbach J M; Hausinger R P
Proteus mirabilis urease. Partial purification and inhibition by boric acid and boronic acids.
The Biochemical journal 1988;250(3):917-20.
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1988: Frei J I; Cavanagh K T; Fisher R A; Hausinger R P; Dupuis M; Rathke E J; Jones M Z
Partial purification of goat kidney beta-mannosidase.
The Biochemical journal 1988;249(3):871-5.
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1987: Todd M J; Hausinger R P
Purification and characterization of the nickel-containing multicomponent urease from Klebsiella aerogenes.
The Journal of biological chemistry 1987;262(13):5963-7.
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