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Robert Hausinger

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TOP 3
Mark A Farrugia; Lee Macomber; Robert P Hausinger
Biosynthesis of the urease metallocenter.
Eric L Carter; Denis A Proshlyakov; Robert P Hausinger
Apoprotein isolation and activation, and vibrational structure of the Helicobacter mustelae iron urease.
Jana M Simmons; Donna J Koslowsky; Robert P Hausinger
Characterization of a Trypanosoma brucei Alkb homolog capable of repairing alkylated DNA.

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All Publications

2013: Mark A Farrugia; Lee Macomber; Robert P Hausinger
Biosynthesis of the urease metallocenter.
The Journal of biological chemistry 2013;288(19):13178-85.
2012: Eric L Carter; Denis A Proshlyakov; Robert P Hausinger
Apoprotein isolation and activation, and vibrational structure of the Helicobacter mustelae iron urease.
Journal of inorganic biochemistry 2012;111():195-202.
2012: Jana M Simmons; Donna J Koslowsky; Robert P Hausinger
Characterization of a Trypanosoma brucei Alkb homolog capable of repairing alkylated DNA.
Experimental parasitology 2012;131(1):92-100.
2012: Jodi L Boer; Robert P Hausinger
Klebsiella aerogenes UreF: identification of the UreG binding site and role in enhancing the fidelity of urease activation.
Biochemistry 2012;51(11):2298-308.
2012: Hajime Masukawa; Hidehiro Sakurai; Kazuhito Inoue; Masaharu Kitashima; Robert P Hausinger
Genetic engineering of cyanobacteria to enhance biohydrogen production from sunlight and water.
Ambio 2012;41 Suppl 2():169-73.
2011: Lee Macomber; Scott P Elsey; Robert P Hausinger
Fructose-1,6-bisphosphate aldolase (class II) is the primary site of nickel toxicity in Escherichia coli.
Molecular microbiology 2011;82(5):1291-300.
2011: Eric L Carter; Mark A Farrugia; Nicholas Flugga; Jodi L Boer; Christopher L Towns; Robert P Hausinger
Function of UreB in Klebsiella aerogenes urease.
Biochemistry 2011;50(43):9296-308.
2011: Lee Macomber; Robert P Hausinger
Mechanisms of nickel toxicity in microorganisms.
Metallomics : integrated biometal science 2011;3(11):1153-62.
2011: Scott B Mulrooney; Michael J Howard; Robert P Hausinger
The Escherichia coli alkylation response protein AidB is a redox partner of flavodoxin and binds RNA and acyl carrier protein.
Archives of biochemistry and biophysics 2011;513(2):81-6.
2011: Eric L Carter; P Andrew Karplus; Scott R Taber; Dale E Tronrud; Robert P Hausinger
Iron-containing urease in a pathogenic bacterium.
Proceedings of the National Academy of Sciences of the United States of America 2011;108(32):13095-9.
2010: Hajime Masukawa; Kazuhito Inoue; Hidehiro Sakurai; C Peter Wolk; Robert P Hausinger
Site-directed mutagenesis of the Anabaena sp. strain PCC 7120 nitrogenase active site to increase photobiological hydrogen production.
Applied and environmental microbiology 2010;76(20):6741-50.
2010: Robert Lam; Vladimir Romanov; Kathy Johns; Kevin P Battaile; Jean Wu-Brown; Jennifer L Guthrie; Robert P Hausinger; Emil F Pai; Nickolay Y Chirgadze
Crystal structure of a truncated urease accessory protein UreF from Helicobacter pylori.
Proteins 2010;78(13):2839-48.
2010: Jodi L Boer; Soledad Quiroz-Valenzuela; Kimberly L Anderson; Robert P Hausinger
Mutagenesis of Klebsiella aerogenes UreG to probe nickel binding and interactions with other urease-related proteins.
Biochemistry 2010;49(28):5859-69.
2010: Eric L Carter; Robert P Hausinger
Characterization of the Klebsiella aerogenes urease accessory protein UreD in fusion with the maltose binding protein.
Journal of bacteriology 2010;192(9):2294-304.
2010: Jana M Simmons; Donna J Koslowsky; Robert P Hausinger
Trypanosoma brucei brucei: thymine 7-hydroxylase-like proteins.
Experimental parasitology 2010;124(4):453-8.
2010: Piotr K Grzyska; Robert P Hausinger; Denis A Proshlyakov
Metal and substrate binding to an Fe(II) dioxygenase resolved by UV spectroscopy with global regression analysis.
Analytical biochemistry 2010;399(1):64-71.
2010: Piotr K Grzyska; Evan H Appelman; Robert P Hausinger; Denis A Proshlyakov
Insight into the mechanism of an iron dioxygenase by resolution of steps following the FeIV=HO species.
Proceedings of the National Academy of Sciences of the United States of America 2010;107(9):3982-7.
2010: Tina A Mueller; Katheryn Meek; Robert P Hausinger
Human AlkB homologue 1 (ABH1) exhibits DNA lyase activity at abasic sites.
DNA repair 2010;9(1):58-65.
2009: Eric L Carter; Nicholas Flugga; Jodi L Boer; Scott B Mulrooney; Robert P Hausinger
Interplay of metal ions and urease.
Metallomics : integrated biometal science 2009;1(3):207-21.
2008: Soledad Quiroz-Valenzuela; Sai Chetan K Sukuru; Robert P Hausinger; Leslie A Kuhn; William T Heller
The structure of urease activation complexes examined by flexibility analysis, mutagenesis, and small-angle X-ray scattering.
Archives of biochemistry and biophysics 2008;480(1):51-7.
2008: Jana M Simmons; Tina A Mueller; Robert P Hausinger
Fe(II)/alpha-ketoglutarate hydroxylases involved in nucleobase, nucleoside, nucleotide, and chromatin metabolism.
Dalton transactions (Cambridge, England : 2003) 2008;(38):5132-42.
2008: Efthalia Kalliri; Scott B Mulrooney; Robert P Hausinger
Identification of Escherichia coli YgaF as an L-2-hydroxyglutarate oxidase.
Journal of bacteriology 2008;190(11):3793-8.
2008: Meng Li; Tina A Mueller; Bruce A Fraser; Robert P Hausinger
Characterization of active site variants of xanthine hydroxylase from Aspergillus nidulans.
Archives of biochemistry and biophysics 2008;470(1):44-53.
2007: Piotr K Grzyska; Robert P Hausinger
Cr(II) reactivity of taurine/alpha-ketoglutarate dioxygenase.
Inorganic chemistry 2007;46(24):10087-92.
2007: Nicholas E Grossoehme; Scott B Mulrooney; Robert P Hausinger; Dean E Wilcox
Thermodynamics of Ni2+, Cu2+, and Zn2+ binding to the urease metallochaperone UreE.
Biochemistry 2007;46(37):10506-16.
2007: Rajendra Bose Muthukumaran; Piotr K Grzyska; Robert P Hausinger; John McCracken
Probing the iron-substrate orientation for taurine/alpha-ketoglutarate dioxygenase using deuterium electron spin echo envelope modulation spectroscopy.
Biochemistry 2007;46(20):5951-9.
2007: Gabriela M Montero-Morán; Meng Li; Erika Rendòn-Huerta; Fabrice Jourdan; David J Lowe; Andrew W Stumpff-Kane; Michael Feig; Claudio Scazzocchio; Robert P Hausinger
Purification and characterization of the FeII- and alpha-ketoglutarate-dependent xanthine hydroxylase from Aspergillus nidulans.
Biochemistry 2007;46(18):5293-304.
2007: Piotr K Grzyska; Tina A Mueller; Melody G Campbell; Robert P Hausinger
Metal ligand substitution and evidence for quinone formation in taurine/alpha-ketoglutarate dioxygenase.
Journal of inorganic biochemistry 2007;101(5):797-808.
2007: Robert P Hausinger
New insights into acetone metabolism.
Journal of bacteriology 2007;189(3):671-3.
2007: Zhong Yu; Paul-André Genest; Bas ter Riet; Kate Sweeney; Courtney DiPaolo; Rudo Kieft; Evangelos Christodoulou; Anastassis Perraks; Jana M Simmons; Robert P Hausinger; Henri G A M van Luenen; Daniel J Rigden; Robert Sabatini; Piet Borst
The protein that binds to DNA base J in trypanosomatids has features of a thymidine hydroxylase.
Nucleic acids research 2007;35(7):2107-15.
2006: Jong Kyong Kim; Scott B Mulrooney; Robert P Hausinger
The UreEF fusion protein provides a soluble and functional form of the UreF urease accessory protein.
Journal of bacteriology 2006;188(24):8413-20.
2006: Li Zhang; Scott B Mulrooney; Andy F K Leung; Yibo Zeng; Ben B C Ko; Robert P Hausinger; Hongzhe Sun
Inhibition of urease by bismuth(III): implications for the mechanism of action of bismuth drugs.
Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine 2006;19(5):503-11.
2006: Tina A Mueller; Maria I Zavodszky; Michael Feig; Leslie A Kuhn; Robert P Hausinger
Structural basis for the enantiospecificities of R- and S-specific phenoxypropionate/alpha-ketoglutarate dioxygenases.
Protein science : a publication of the Protein Society 2006;15(6):1356-68.
2006: Lusong Luo; Melissa B Pappalardi; Peter J Tummino; Robert Copeland; Marie E Fraser; Piotr K Grzyska; Robert P Hausinger
An assay for Fe(II)/2-oxoglutarate-dependent dioxygenases by enzyme-coupled detection of succinate formation.
Analytical biochemistry 2006;353(1):69-74.
2006: Mukta S Rohankhedkar; Scott B Mulrooney; William J Wedemeyer; Robert P Hausinger
The AidB component of the Escherichia coli adaptive response to alkylating agents is a flavin-containing, DNA-binding protein.
Journal of bacteriology 2006;188(1):223-30.
2006: Kevin D Koehntop; Sudha Marimanikkuppam; Matthew J Ryle; Robert P Hausinger; Lawrence Que
Self-hydroxylation of taurine/alpha-ketoglutarate dioxygenase: evidence for more than one oxygen activation mechanism.
Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry 2006;11(1):63-72.
2005: Efthalia Kalliri; Piotr K Grzyska; Robert P Hausinger
Kinetic and spectroscopic investigation of CoII, NiII, and N-oxalylglycine inhibition of the FeII/alpha-ketoglutarate dioxygenase, TauD.
Biochemical and biophysical research communications 2005;338(1):191-7.
2005: Jong Kyong Kim; Scott B Mulrooney; Robert P Hausinger
Biosynthesis of active Bacillus subtilis urease in the absence of known urease accessory proteins.
Journal of bacteriology 2005;187(20):7150-4.
2005: Scott B Mulrooney; Sarah K Ward; Robert P Hausinger
Purification and properties of the Klebsiella aerogenes UreE metal-binding domain, a functional metallochaperone of urease.
Journal of bacteriology 2005;187(10):3581-5.
2005: Piotr K Grzyska; Matthew J Ryle; Greta R Monterosso; Jian Liu; David P Ballou; Robert P Hausinger
Steady-state and transient kinetic analyses of taurine/alpha-ketoglutarate dioxygenase: effects of oxygen concentration, alternative sulfonates, and active-site variants on the FeIV-oxo intermediate.
Biochemistry 2005;44(10):3845-55.
2004: Timothy F Henshaw; Michael Feig; Robert P Hausinger
Aberrant activity of the DNA repair enzyme AlkB.
Journal of inorganic biochemistry 2004;98(5):856-61.
2004: Robert P Hausinger
Metabolic versatility of prokaryotes for urea decomposition.
Journal of bacteriology 2004;186(9):2520-2.
2004: Zhenzhan Chang; Jason Kuchar; Robert P Hausinger
Chemical cross-linking and mass spectrometric identification of sites of interaction for UreD, UreF, and urease.
The Journal of biological chemistry 2004;279(15):15305-13.
2004: Denis A Proshlyakov; Timothy F Henshaw; Greta R Monterosso; Matthew J Ryle; Robert P Hausinger
Direct detection of oxygen intermediates in the non-heme Fe enzyme taurine/alpha-ketoglutarate dioxygenase.
Journal of the American Chemical Society 2004;126(4):1022-3.
2004: Jason Kuchar; Robert P Hausinger
Biosynthesis of metal sites.
Chemical reviews 2004;104(2):509-25.
2004: Robert P Hausinger
FeII/alpha-ketoglutarate-dependent hydroxylases and related enzymes.
Critical reviews in biochemistry and molecular biology 2004;39(1):21-68.
2003: Scott B Mulrooney; Robert P Hausinger
Nickel uptake and utilization by microorganisms.
FEMS microbiology reviews 2003;27(2-3):239-61.
2003: Robert P Hausinger
Ni and CO: more surprises.
Nature structural biology 2003;10(4):234-6.
2003: Matthew J Ryle; Kevin D Koehntop; Aimin Liu; Lawrence Que; Robert P Hausinger
Interconversion of two oxidized forms of taurine/alpha-ketoglutarate dioxygenase, a non-heme iron hydroxylase: evidence for bicarbonate binding.
Proceedings of the National Academy of Sciences of the United States of America 2003;100(7):3790-5.
2003: Matthew J Ryle; Aimin Liu; Rajendra Bose Muthukumaran; Raymond Y N Ho; Kevin D Koehntop; John McCracken; Lawrence Que; Robert P Hausinger
O2- and alpha-ketoglutarate-dependent tyrosyl radical formation in TauD, an alpha-keto acid-dependent non-heme iron dioxygenase.
Biochemistry 2003;42(7):1854-62.
2003: Julie C Hotopp; Thomas A Auchtung; Deborah A Hogan; Robert P Hausinger
Intrinsic tryptophan fluorescence as a probe of metal and alpha-ketoglutarate binding to TfdA, a mononuclear non-heme iron dioxygenase.
Journal of inorganic biochemistry 2003;93(1-2):66-70.
2003: Scott B Mulrooney; Robert P Hausinger
Metal ion dependence of recombinant Escherichia coli allantoinase.
Journal of bacteriology 2003;185(1):126-34.
2002: Sarah C Trewick; Timothy F Henshaw; Robert P Hausinger; Tomas Lindahl; Barbara Sedgwick
Oxidative demethylation by Escherichia coli AlkB directly reverts DNA base damage.
Nature 2002;419(6903):174-8.
2002: Julie C Hotopp; Robert P Hausinger
Probing the 2,4-dichlorophenoxyacetate/alpha-ketoglutarate dioxygenase substrate-binding site by site-directed mutagenesis and mechanism-based inactivation.
Biochemistry 2002;41(31):9787-94.
2002: Kazuhito Itoh; Rie Kanda; Yoko Sumita; Hongik Kim; Yoichi Kamagata; Kousuke Suyama; Hiroki Yamamoto; Robert P Hausinger; James M Tiedje
tfdA-like genes in 2,4-dichlorophenoxyacetic acid-degrading bacteria belonging to the Bradyrhizobium-Agromonas-Nitrobacter-Afipia cluster in alpha-Proteobacteria.
Applied and environmental microbiology 2002;68(7):3449-54.
2002: Jonathan M Elkins; Matthew J Ryle; Ian J Clifton; Julie C Hotopp; John S Lloyd; Nicolai I Burzlaff; Jack E Baldwin; Robert P Hausinger; Peter L Roach
X-ray crystal structure of Escherichia coli taurine/alpha-ketoglutarate dioxygenase complexed to ferrous iron and substrates.
Biochemistry 2002;41(16):5185-92.
2002: Matthew J Ryle; Robert P Hausinger
Non-heme iron oxygenases.
Current opinion in chemical biology 2002;6(2):193-201.
2001: H K Song; Scott B Mulrooney; Robert Huber; Robert P Hausinger
Crystal structure of Klebsiella aerogenes UreE, a nickel-binding metallochaperone for urease activation.
The Journal of biological chemistry 2001;276(52):49359-64.
2001: Scott B Mulrooney; T Zakharian; RA Schaller; Robert P Hausinger
Dual effects of ionic strength on Klebsiella aerogenes urease: pH-dependent activation and inhibition.
Archives of biochemistry and biophysics 2001;394(2):280-2.
2001: Aimin Liu; Raymond Y N Ho; Lawrence Que; Matthew J Ryle; Brett Phinney; Robert P Hausinger
Alternative reactivity of an alpha-ketoglutarate-dependent iron(II) oxygenase: enzyme self-hydroxylation.
Journal of the American Chemical Society 2001;123(21):5126-7.
2001: Raymond Y N Ho; Mark P Mehn; Eric L Hegg; Aimin Liu; Matthew J Ryle; Robert P Hausinger; Lawrence Que
Resonance Raman studies of the iron(II)--alpha-keto acid chromophore in model and enzyme complexes.
Journal of the American Chemical Society 2001;123(21):5022-9.
2000: A Soriano; GJ Colpas; Robert P Hausinger
UreE stimulation of GTP-dependent urease activation in the UreD-UreF-UreG-urease apoprotein complex.
Biochemistry 2000;39(40):12435-40.
2000: MA Pearson; I S Park; RA Schaller; LO Michel; P Andrew Karplus; Robert P Hausinger
Kinetic and structural characterization of urease active site variants.
Biochemistry 2000;39(29):8575-84.
2000: M J Todd; Robert P Hausinger
Fluoride inhibition of Klebsiella aerogenes urease: mechanistic implications of a pseudo-uncompetitive, slow-binding inhibitor.
Biochemistry 2000;39(18):5389-96.
2000: Deborah A Hogan; S R Smith; E A Saari; John McCracken; Robert P Hausinger
Site-directed mutagenesis of 2,4-dichlorophenoxyacetic acid/alpha-ketoglutarate dioxygenase. Identification of residues involved in metallocenter formation and substrate binding.
The Journal of biological chemistry 2000;275(17):12400-9.
2000: GJ Colpas; Robert P Hausinger
In vivo and in vitro kinetics of metal transfer by the Klebsiella aerogenes urease nickel metallochaperone, UreE.
The Journal of biological chemistry 2000;275(15):10731-7.
1999: Eric L Hegg; A K Whiting; RE Saari; John McCracken; Robert P Hausinger; Lawrence Que
Herbicide-degrading alpha-keto acid-dependent enzyme TfdA: metal coordination environment and mechanistic insights.
Biochemistry 1999;38(50):16714-26.
1999: Matthew J Ryle; R Padmakumar; Robert P Hausinger
Stopped-flow kinetic analysis of Escherichia coli taurine/alpha-ketoglutarate dioxygenase: interactions with alpha-ketoglutarate, taurine, and oxygen.
Biochemistry 1999;38(46):15278-86.
1999: A Soriano; Robert P Hausinger
GTP-dependent activation of urease apoprotein in complex with the UreD, UreF, and UreG accessory proteins.
Proceedings of the National Academy of Sciences of the United States of America 1999;96(20):11140-4.
1999: Deborah A Hogan; T A Auchtung; Robert P Hausinger
Cloning and characterization of a sulfonate/alpha-ketoglutarate dioxygenase from Saccharomyces cerevisiae.
Journal of bacteriology 1999;181(18):5876-9.
1999: K Yamaguchi; Nathaniel J Cosper; CM Stålhandske; Robert A Scott; MA Pearson; P Andrew Karplus; Robert P Hausinger
Characterization of metal-substituted Klebsiella aerogenes urease.
Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry 1999;4(4):468-77.
1999: GJ Colpas; T G Brayman; L J Ming; Robert P Hausinger
Identification of metal-binding residues in the Klebsiella aerogenes urease nickel metallochaperone, UreE.
Biochemistry 1999;38(13):4078-88.
1999: V Romanov; M T Merski; Robert P Hausinger
Assays for allantoinase.
Analytical biochemistry 1999;268(1):49-53.
1999: Nathaniel J Cosper; CM Stålhandske; RE Saari; Robert P Hausinger; Robert A Scott
X-ray absorption spectroscopic analysis of Fe(II) and Cu(II) forms of a herbicide-degrading alpha-ketoglutarate dioxygenase.
Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry 1999;4(1):122-9.
1998: MA Pearson; RA Schaller; LO Michel; P Andrew Karplus; Robert P Hausinger
Chemical rescue of Klebsiella aerogenes urease variants lacking the carbamylated-lysine nickel ligand.
Biochemistry 1998;37(17):6214-20.
1998: RE Saari; Robert P Hausinger
Ascorbic acid-dependent turnover and reactivation of 2,4-dichlorophenoxyacetic acid/alpha-ketoglutarate dioxygenase using thiophenoxyacetic acid.
Biochemistry 1998;37(9):3035-42.
1997: K Yamaguchi; Robert P Hausinger
Substitution of the urease active site carbamate by dithiocarbamate and vanadate.
Biochemistry 1997;36(49):15118-22.
1997: M B Moncrief; Robert P Hausinger
Characterization of UreG, identification of a UreD-UreF-UreG complex, and evidence suggesting that a nucleotide-binding site in UreG is required for in vivo metallocenter assembly of Klebsiella aerogenes urease.
Journal of bacteriology 1997;179(13):4081-6.
1997: MA Pearson; LO Michel; Robert P Hausinger; P Andrew Karplus
Structures of Cys319 variants and acetohydroxamate-inhibited Klebsiella aerogenes urease.
Biochemistry 1997;36(26):8164-72.
1997: T M Sassanella; F Fukumori; M Bagdasarian; Robert P Hausinger
Use of 4-Nitrophenoxyacetic Acid for Detection and Quantification of 2,4-Dichlorophenoxyacetic Acid (2,4-D)/(alpha)-Ketoglutarate Dioxygenase Activity in 2,4-D-Degrading Microorganisms.
Applied and environmental microbiology 1997;63(3):1189-91.
1996: M B Moncrief; Robert P Hausinger
Purification and activation properties of UreD-UreF-urease apoprotein complexes.
Journal of bacteriology 1996;178(18):5417-21.
1996: T G Brayman; Robert P Hausinger
Purification, characterization, and functional analysis of a truncated Klebsiella aerogenes UreE urease accessory protein lacking the histidine-rich carboxyl terminus.
Journal of bacteriology 1996;178(18):5410-6.
1996: I S Park; L O Michel; MA Pearson; E Jabri; P Andrew Karplus; S Wang; J Dong; Robert A Scott; B P Koehler; Michael K Johnson; Robert P Hausinger
Characterization of the mononickel metallocenter in H134A mutant urease.
The Journal of biological chemistry 1996;271(31):18632-7.
1996: Y Suwa; A D Wright; F Fukimori; K A Nummy; Robert P Hausinger; William Holben; LJ Forney
Characterization of a chromosomally encoded 2,4-dichlorophenoxyacetic acid/alpha-ketoglutarate dioxygenase from Burkholderia sp. strain RASC.
Applied and environmental microbiology 1996;62(7):2464-9.
1996: V Romanov; Robert P Hausinger
NADPH-dependent reductive ortho dehalogenation of 2,4-dichlorobenzoic acid in Corynebacterium sepedonicum KZ-4 and Coryneform bacterium strainNTB-1 via 2,4-dichlorobenzoyl coenzyme A.
Journal of bacteriology 1996;178(9):2656-61.
1996: I S Park; Robert P Hausinger
Metal ion interaction with urease and UreD-urease apoproteins.
Biochemistry 1996;35(16):5345-52.
1995: M B Moncrief; L G Hom; E Jabri; P Andrew Karplus; Robert P Hausinger
Urease activity in the crystalline state.
Protein science : a publication of the Protein Society 1995;4(10):2234-6.
1995: harry mobley; MD Island; Robert P Hausinger
Molecular biology of microbial ureases.
Microbiological reviews 1995;59(3):451-80.
1995: Robert P Hausinger; F Fukumori
Characterization of the first enzyme in 2,4-dichlorophenoxyacetic acid metabolism.
Environmental health perspectives 1995;103 Suppl 5():37-9.
1995: E Jabri; M B Carr; Robert P Hausinger; P Andrew Karplus
The crystal structure of urease from Klebsiella aerogenes.
Science (New York, N.Y.) 1995;268(5213):998-1004.
1995: I S Park; Robert P Hausinger
Evidence for the presence of urease apoprotein complexes containing UreD, UreF, and UreG in cells that are competent for in vivo enzyme activation.
Journal of bacteriology 1995;177(8):1947-51.
1995: I S Park; Robert P Hausinger
Requirement of carbon dioxide for in vitro assembly of the urease nickel metallocenter.
Science (New York, N.Y.) 1995;267(5201):1156-8.
1994: Robert P Hausinger
Nickel enzymes in microbes.
The Science of the total environment 1994;148(2-3):157-66.
1994: V Romanov; Robert P Hausinger
Pseudomonas aeruginosa 142 uses a three-component ortho-halobenzoate 1,2-dioxygenase for metabolism of 2,4-dichloro- and 2-chlorobenzoate.
Journal of bacteriology 1994;176(11):3368-74.
1994: I S Park; M B Carr; Robert P Hausinger
In vitro activation of urease apoprotein and role of UreD as a chaperone required for nickel metallocenter assembly.
Proceedings of the National Academy of Sciences of the United States of America 1994;91(8):3233-7.
1993: F Fukumori; Robert P Hausinger
Purification and characterization of 2,4-dichlorophenoxyacetate/alpha-ketoglutarate dioxygenase.
The Journal of biological chemistry 1993;268(32):24311-7.
1993: M H Lee; H S Pankratz; S Wang; Robert A Scott; M G Finnegan; Michael K Johnson; J A Ippolito; David W Christianson; Robert P Hausinger
Purification and characterization of Klebsiella aerogenes UreE protein: a nickel-binding protein that functions in urease metallocenter assembly.
Protein science : a publication of the Protein Society 1993;2(6):1042-52.
1993: I S Park; Robert P Hausinger
Site-directed mutagenesis of Klebsiella aerogenes urease: identification of histidine residues that appear to function in nickel ligation, substrate binding, and catalysis.
Protein science : a publication of the Protein Society 1993;2(6):1034-41.
1993: F Fukumori; Robert P Hausinger
Alcaligenes eutrophus JMP134 "2,4-dichlorophenoxyacetate monooxygenase" is an alpha-ketoglutarate-dependent dioxygenase.
Journal of bacteriology 1993;175(7):2083-6.
1993: I S Park; Robert P Hausinger
Diethylpyrocarbonate reactivity of Klebsiella aerogenes urease: effect of pH and active site ligands on the rate of inactivation.
Journal of protein chemistry 1993;12(1):51-6.
1992: E Jabri; M H Lee; Robert P Hausinger; P Andrew Karplus
Preliminary crystallographic studies of urease from jack bean and from Klebsiella aerogenes.
Journal of molecular biology 1992;227(3):934-7.
1992: P R Martin; Robert P Hausinger
Site-directed mutagenesis of the active site cysteine in Klebsiella aerogenes urease.
The Journal of biological chemistry 1992;267(28):20024-7.
1992: M H Lee; S B Mulrooney; M J Renner; Y Markowicz; Robert P Hausinger
Klebsiella aerogenes urease gene cluster: sequence of ureD and demonstration that four accessory genes (ureD, ureE, ureF, and ureG) are involved in nickel metallocenter biosynthesis.
Journal of bacteriology 1992;174(13):4324-30.
1992: D D McCoy; A Cetin; Robert P Hausinger
Characterization of urease from Sporosarcina ureae.
Archives of microbiology 1992;157(5):411-6.
1991: M J Todd; Robert P Hausinger
Identification of the essential cysteine residue in Klebsiella aerogenes urease.
The Journal of biological chemistry 1991;266(36):24327-31.
1991: M J Todd; Robert P Hausinger
Reactivity of the essential thiol of Klebsiella aerogenes urease. Effect of pH and ligands on thiol modification.
The Journal of biological chemistry 1991;266(16):10260-7.
1990: S B Mulrooney; Robert P Hausinger
Sequence of the Klebsiella aerogenes urease genes and evidence for accessory proteins facilitating nickel incorporation.
Journal of bacteriology 1990;172(10):5837-43.
1990: M H Lee; S B Mulrooney; Robert P Hausinger
Purification, characterization, and in vivo reconstitution of Klebsiella aerogenes urease apoenzyme.
Journal of bacteriology 1990;172(8):4427-31.
1990: Robert P Hausinger
Mechanisms of metal ion incorporation into metalloproteins.
BioFactors (Oxford, England) 1990;2(3):179-84.
1989: M J Todd; Robert P Hausinger
Competitive inhibitors of Klebsiella aerogenes urease. Mechanisms of interaction with the nickel active site.
The Journal of biological chemistry 1989;264(27):15835-42.
1989: S B Mulrooney; H S Pankratz; Robert P Hausinger
Regulation of gene expression and cellular localization of cloned Klebsiella aerogenes (K. pneumoniae) urease.
Journal of general microbiology 1989;135(6):1769-76.
1989: harry mobley; Robert P Hausinger
Microbial ureases: significance, regulation, and molecular characterization.
Microbiological reviews 1989;53(1):85-108.
1988: Lisa M Gloss; Robert P Hausinger
Methanogen factor 390 formation: species distribution, reversibility and effects of non-oxidative cellular stresses.
BioFactors (Oxford, England) 1988;1(3):237-40.
1988: S B Mulrooney; M J Lynch; harry mobley; Robert P Hausinger
Purification, characterization, and genetic organization of recombinant Providencia stuartii urease expressed by Escherichia coli.
Journal of bacteriology 1988;170(5):2202-7.
1988: D A Simpson; Robert P Hausinger; Martha H Mulks
Purification, characterization, and comparison of the immunoglobulin A1 proteases of Neisseria gonorrhoeae.
Journal of bacteriology 1988;170(4):1866-73.
1988: J M Breitenbach; Robert P Hausinger
Proteus mirabilis urease. Partial purification and inhibition by boric acid and boronic acids.
The Biochemical journal 1988;250(3):917-20.
1988: J I Frei; Kevin T Cavanagh; R A Fisher; Robert P Hausinger; M Dupuis; E J Rathke; Margaret Z Jones
Partial purification of goat kidney beta-mannosidase.
The Biochemical journal 1988;249(3):871-5.
1987: M J Todd; Robert P Hausinger
Purification and characterization of the nickel-containing multicomponent urease from Klebsiella aerogenes.
The Journal of biological chemistry 1987;262(13):5963-7.
1987: Robert P Hausinger
Nickel utilization by microorganisms.
Microbiological reviews 1987;51(1):22-42.
1986: Robert P Hausinger
Purification of a nickel-containing urease from the rumen anaerobe Selenomonas ruminantium.
The Journal of biological chemistry 1986;261(17):7866-70.
1986: Robert P Hausinger; J F Honek; C Walsh
Separation of flavins and flavin analogs by high-performance liquid chromatography.
Methods in enzymology 1986;122():199-209.
1985: Robert P Hausinger; W H Orme-Johnson; C Walsh
Factor 390 chromophores: phosphodiester between AMP or GMP and methanogen factor 420.
Biochemistry 1985;24(7):1629-33.
1983: Robert P Hausinger; J B Howard
Thiol reactivity of the nitrogenase Fe-protein from Azotobacter vinelandii.
The Journal of biological chemistry 1983;258(22):13486-92.
1983: N Kojima; J A Fox; Robert P Hausinger; Lacy Daniels; W H Orme-Johnson; C Walsh
Paramagnetic centers in the nickel-containing, deazaflavin-reducing hydrogenase from Methanobacterium thermoautotrophicum.
Proceedings of the National Academy of Sciences of the United States of America 1983;80(2):378-82.
1982: Robert P Hausinger; I Moura; J J Moura; A V Xavier; M H Santos; J LeGall; J B Howard
Amino acid sequence of a 3Fe:3S ferredoxin from the "archaebacterium" Methanosarcina barkeri (DSM 800).
The Journal of biological chemistry 1982;257(23):14192-7.
1982: Robert P Hausinger; J B Howard
The amino acid sequence of the nitrogenase iron protein from Azotobacter vinelandii.
The Journal of biological chemistry 1982;257(5):2483-90.
1980: Robert P Hausinger; J B Howard
Comparison of the iron proteins from the nitrogen fixation complexes of Azotobacter vinelandii, Clostridium pasteurianum, and Klebsiella pneumoniae.
Proceedings of the National Academy of Sciences of the United States of America 1980;77(7):3826-30.
1980: Isabel Moura; Boi Hanh Huynh; Robert P Hausinger; J Le Gall; António V Xavier; E Münck
Mössbauer and EPR studies of desulforedoxin from Desulfovibrio gigas.
The Journal of biological chemistry 1980;255(6):2493-8.