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Wolfgang Hillen

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TOP 3
Cornelius Wimmer; Stephanie Platzer; Wolfgang Hillen; Marcus Klotzsche
A novel method to analyze nucleocytoplasmic transport in vivo by using short Peptide tags.
Christoph Stoeckle; Marcus Klotzsche; Wolfgang Hillen
Protein expression can be monitored in yeast by peptide-mediated induction of TetR-controlled gene expression.
Andrea Wünsche; Gerald Seidel; Uwe Völker; Maike Bartholomae; Andreas Burkovski; Elke Hammer; Wolfgang Hillen
CcpA forms complexes with CodY and RpoA in Bacillus subtilis.

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All Publications

2013: Cornelius Wimmer; Stephanie Platzer; Wolfgang Hillen; Marcus Klotzsche
A novel method to analyze nucleocytoplasmic transport in vivo by using short Peptide tags.
Journal of molecular biology 2013;425(10):1839-45.
2012: Christoph Stoeckle; Marcus Klotzsche; Wolfgang Hillen
Protein expression can be monitored in yeast by peptide-mediated induction of TetR-controlled gene expression.
Journal of biotechnology 2012;161(3):265-8.
2012: Andrea Wünsche; Gerald Seidel; Uwe Völker; Maike Bartholomae; Andreas Burkovski; Elke Hammer; Wolfgang Hillen
CcpA forms complexes with CodY and RpoA in Bacillus subtilis.
The FEBS journal 2012;279(12):2201-14.
2012: Dagmar Goeke; Sandra Grubmüller; Daniela Kaspar; Marcus Klotzsche; Christoph Stoeckle; Christian Berens; Wolfgang Hillen
Short peptides act as inducers, anti-inducers and corepressors of Tet repressor.
Journal of molecular biology 2012;416(1):33-45.
2012: Madhumati Sevvana; Cornelius Wimmer; Christian Berens; Dagmar Goeke; Christoph Goetz; Wolfgang Hillen; Yves A Muller
An exclusive α/β code directs allostery in TetR-peptide complexes.
Journal of molecular biology 2012;416(1):46-56.
2012: Christiane Georgi; Wolfgang Hillen; Julia Buerger; Christian Berens
Promoter strength driving TetR determines the regulatory properties of Tet-controlled expression systems.
PloS one 2012;7(7):e41620.
2011: Maria A Schumacher; Mareen Sprehe; Maike Bartholomae; Wolfgang Hillen; Richard G Brennan
Structures of carbon catabolite protein A-(HPr-Ser46-P) bound to diverse catabolite response element sites reveal the basis for high-affinity binding to degenerate DNA operators.
Nucleic acids research 2011;39(7):2931-42.
2011: Ingo Schubert; Christian Kellner; Christoph Stein; Markus Kügler; Michael Schwenkert; Domenica Saul; Kristin Mentz; Heiko Singer; Bernhard Stockmeyer; Wolfgang Hillen; Andreas Mackensen; Georg H Fey
A single-chain triplebody with specificity for CD19 and CD33 mediates effective lysis of mixed lineage leukemia cells by dual targeting.
mAbs 2011;3(1):21-30.
2010: Susanne Lochner; Jürgen Einsiedel; Gesa Schaefer; Christian Berens; Wolfgang Hillen; Peter Gmeiner
Anhydrotetracycline-peptide conjugates as representatives for ligand-based transactivating systems.
Bioorganic & medicinal chemistry 2010;18(16):6127-33.
2010: Christina Danke; Xandra Grünz; Jürgen Wittmann; Andreas Schmidt; Siamak Agha-Mohammadi; Olaf Kutsch; Hans-Martin Jäck; Wolfgang Hillen; Christian Berens
Adjusting transgene expression levels in lymphocytes with a set of inducible promoters.
The journal of gene medicine 2010;12(6):501-15.
2010: Igor Usai; Marcus Krueger; Jürgen Einsiedel; Wolfgang Hillen; Peter Gmeiner
Click-chemistry-derived tetracycline-amino acid conjugates exhibiting exceptional potency and exclusive recognition of the reverse tet repressor.
Chembiochem : a European journal of chemical biology 2010;11(5):703-12.
2009: Christian Kormann; Irina Pimenta; Stefan Löber; Cornelius Wimmer; Harald Lanig; Timothy Clark; Wolfgang Hillen; Peter Gmeiner
Diarylpropane-1,3-dione derivatives as TetR-inducing tetracycline mimetics: Synthesis and biological investigations.
Chembiochem : a European journal of chemical biology 2009;10(18):2924-33.
2009: Michael Alexander Klieber; Oliver Scholz; Susanne Lochner; Peter Gmeiner; Wolfgang Hillen; Yves A Muller
Structural origins for selectivity and specificity in an engineered bacterial repressor-inducer pair.
The FEBS journal 2009;276(19):5610-21.
2009: Anke Hunsicker; Markus Steber; Günter Mayer; Johannes Meitert; Marcus Klotzsche; Michael Blind; Wolfgang Hillen; Christian Berens; Beatrix Suess
An RNA aptamer that induces transcription.
Chemistry & biology 2009;16(2):173-80.
2008: Janko Daam; Kaoutar Mehdaoui; Marcus Klotzsche; Klaus Pfleiderer; Christian Berens; Wolfgang Hillen
Functionally important residues of the Tet repressor inducing peptide TIP determined by a complete mutational analysis.
Gene 2008;423(2):201-6.
2008: Gottfried J Palm; Thomas Lederer; Peter Orth; Wolfram Saenger; Masayuki Takahashi; Wolfgang Hillen; Winfried Hinrichs
Specific binding of divalent metal ions to tetracycline and to the Tet repressor/tetracycline complex.
Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry 2008;13(7):1097-110.
2008: Marcus Resch; Harald Striegl; Eva-Maria Henssler; Madhumati Sevvana; Claudia Egerer-Sieber; Emile Schiltz; Wolfgang Hillen; Yves A Muller
A protein functional leap: how a single mutation reverses the function of the transcription regulator TetR.
Nucleic acids research 2008;36(13):4390-401.
2008: Ralph Bertram; Wolfgang Hillen
The application of Tet repressor in prokaryotic gene regulation and expression.
Microbial biotechnology 2008;1(1):2-16.
2007: Sheetal Gandotra; Dirk Schnappinger; Mercedes Monteleone; Wolfgang Hillen; Sabine Ehrt
In vivo gene silencing identifies the Mycobacterium tuberculosis proteasome as essential for the bacteria to persist in mice.
Nature medicine 2007;13(12):1515-20.
2007: Marcus Krueger; Oliver Scholz; Stefanie Wisshak; Wolfgang Hillen
Engineered Tet repressors with recognition specificity for the tetO-4C5G operator variant.
Gene 2007;404(1-2):93-100.
2007: Xinzheng V Guo; Mercedes Monteleone; Marcus Klotzsche; Annette Kamionka; Wolfgang Hillen; Miriam Braunstein; Sabine Ehrt; Dirk Schnappinger
Silencing Mycobacterium smegmatis by using tetracycline repressors.
Journal of bacteriology 2007;189(13):4614-23.
2007: M Alper Selver; Felix Fischer; Mehmet Kuntalp; Wolfgang Hillen
A software tool for interactive generation, representation, and systematical storage of transfer functions for 3D medical images.
Computer methods and programs in biomedicine 2007;86(3):270-80.
2007: Maria A Schumacher; Gerald Seidel; Wolfgang Hillen; Richard G Brennan
Structural mechanism for the fine-tuning of CcpA function by the small molecule effectors glucose 6-phosphate and fructose 1,6-bisphosphate.
Journal of molecular biology 2007;368(4):1042-50.
2007: Sylvia R Luckner; Marcus Klotzsche; Christian Berens; Wolfgang Hillen; Yves A Muller
How an agonist peptide mimics the antibiotic tetracycline to induce Tet-repressor.
Journal of molecular biology 2007;368(3):780-90.
2007: Nicola Horstmann; Gerald Seidel; Lwin-Mar Aung-Hilbrich; Wolfgang Hillen
Residues His-15 and Arg-17 of HPr participate differently in catabolite signal processing via CcpA.
The Journal of biological chemistry 2007;282(2):1175-82.
2007: Marcus Klotzsche; Dagmar Goeke; Christian Berens; Wolfgang Hillen
Efficient and exclusive induction of Tet repressor by the oligopeptide Tip results from co-variation of their interaction site.
Nucleic acids research 2007;35(12):3945-52.
2007: Xue Zhou; Jori Symons; Rieuwert Hoppes; Christel Krueger; Christian Berens; Wolfgang Hillen; Ben Berkhout; Atze T Das
Improved single-chain transactivators of the Tet-On gene expression system.
BMC biotechnology 2007;7():6.
2007: Mareen Sprehe; Gerald Seidel; Marco Diel; Wolfgang Hillen
CcpA mutants with differential activities in Bacillus subtilis.
Journal of molecular microbiology and biotechnology 2007;12(1-2):96-105.
2007: Sonja Mertins; Biju Joseph; Monika Goetz; Regina Ecke; Gerald Seidel; Mareen Sprehe; Wolfgang Hillen; Werner Goebel; Stefanie Müller-Altrock
Interference of components of the phosphoenolpyruvate phosphotransferase system with the central virulence gene regulator PrfA of Listeria monocytogenes.
Journal of bacteriology 2007;189(2):473-90.
2006: Norman Mauder; Regina Ecke; Sonja Mertins; Daniela I M Loeffler; Gerald Seidel; Mareen Sprehe; Wolfgang Hillen; Werner Goebel; Stefanie Müller-Altrock
Species-specific differences in the activity of PrfA, the key regulator of listerial virulence genes.
Journal of bacteriology 2006;188(22):7941-56.
2006: Christian Berens; Susanne Lochner; Stefan Löber; Igor Usai; Andreas Schmidt; Lars Drueppel; Wolfgang Hillen; Peter Gmeiner
Subtype selective tetracycline agonists and their application for a two-stage regulatory system.
Chembiochem : a European journal of chemical biology 2006;7(9):1320-4.
2006: Maximilian Schlicht; Christian Berens; Janko Daam; Wolfgang Hillen
Random insertion of a TetR-inducing peptide tag into Escherichia coli proteins allows analysis of protein levels by induction of reporter gene expression.
Applied and environmental microbiology 2006;72(8):5637-42.
2006: Christel Krueger; Christina Danke; Klaus Pfleiderer; Wolfgang Schuh; Hans-Martin Jäck; Susanne Lochner; Peter Gmeiner; Wolfgang Hillen; Christian Berens
A gene regulation system with four distinct expression levels.
The journal of gene medicine 2006;8(8):1037-47.
2006: Martin Köstner; Britta Schmidt; Ralph Bertram; Wolfgang Hillen
Generating tetracycline-inducible auxotrophy in Escherichia coli and Salmonella enterica serovar Typhimurium by using an insertion element and a hyperactive transposase.
Applied and environmental microbiology 2006;72(7):4717-25.
2006: Ralph Bertram; Andrea Wünsche; Mareen Sprehe; Wolfgang Hillen
Regulated expression of HPrK/P does not affect carbon catabolite repression of the xyn operon and of rocG in Bacillus subtilis.
FEMS microbiology letters 2006;259(1):147-52.
2006: Maria A Schumacher; Gerald Seidel; Wolfgang Hillen; Richard G Brennan
Phosphoprotein Crh-Ser46-P displays altered binding to CcpA to effect carbon catabolite regulation.
The Journal of biological chemistry 2006;281(10):6793-800.
2006: Wolfgang Müller; Nicola Horstmann; Wolfgang Hillen; Heinrich Sticht
The transcription regulator RbsR represents a novel interaction partner of the phosphoprotein HPr-Ser46-P in Bacillus subtilis.
The FEBS journal 2006;273(6):1251-61.
2006: Annette Kamionka; Marius Majewski; Karin Roth; Ralph Bertram; Christine Kraft; Wolfgang Hillen
Induction of single chain tetracycline repressor requires the binding of two inducers.
Nucleic acids research 2006;34(14):3834-41.
2006: Chris Goldring; Neil R Kitteringham; Rosalind Jenkins; Cerys A Lovatt; Laura E Randle; Azman Abdullah; Andrew Owen; Xiaoping Liu; Philip J Butler; Dominic P Williams; Peter Metcalfe; Christian Berens; Wolfgang Hillen; Brian Foster; Alec Simpson; Lesley McLellan; B Kevin Park
Development of a transactivator in hepatoma cells that allows expression of phase I, phase II, and chemical defense genes.
American journal of physiology. Cell physiology 2006;290(1):C104-15.
2005: Eva-Maria Henssler; Ralph Bertram; Stefanie Wisshak; Wolfgang Hillen
Tet repressor mutants with altered effector binding and allostery.
The FEBS journal 2005;272(17):4487-96.
2005: Marcus Klotzsche; Christian Berens; Wolfgang Hillen
A peptide triggers allostery in tet repressor by binding to a unique site.
The Journal of biological chemistry 2005;280(26):24591-9.
2005: Gerald Seidel; Marco Diel; Norbert Fuchsbauer; Wolfgang Hillen
Quantitative interdependence of coeffectors, CcpA and cre in carbon catabolite regulation of Bacillus subtilis.
The FEBS journal 2005;272(10):2566-77.
2005: Anja Knott; Lars Drueppel; Thomas Beyer; Kristin Garke; Christian Berens; Martin Herrmann; Wolfgang Hillen
An optimized conditional suicide switch using doxycycline-dependent expression of human tBid.
Cancer biology & therapy 2005;4(5):532-6.
2005: Annette Kamionka; Ralph Bertram; Wolfgang Hillen
Tetracycline-dependent conditional gene knockout in Bacillus subtilis.
Applied and environmental microbiology 2005;71(2):728-33.
2005: Stefan Ludwig Ameres; Lars Drueppel; Klaus Pfleiderer; Andreas Schmidt; Wolfgang Hillen; Christian Berens
Inducible DNA-loop formation blocks transcriptional activation by an SV40 enhancer.
The EMBO journal 2005;24(2):358-67.
2005: Ralph Bertram; Martin Köstner; Judith Müller; José Vazquez Ramos; Wolfgang Hillen
Integrative elements for Bacillus subtilis yielding tetracycline-dependent growth phenotypes.
Nucleic acids research 2005;33(18):e153.
2005: Ee-Tsin Wong; John L Kolman; Yao-Cheng Li; Larry D Mesner; Wolfgang Hillen; Christian Berens; Geoffrey M Wahl
Reproducible doxycycline-inducible transgene expression at specific loci generated by Cre-recombinase mediated cassette exchange.
Nucleic acids research 2005;33(17):e147.
2005: Georg W Bornkamm; Christian Berens; Conny Kuklik-Roos; Jean-Marie Bechet; Gerhard Laux; Jürgen Bachl; Martin Korndoerfer; Martin Schlee; Michael Hölzel; Anastassia Malamoussi; Rob D Chapman; Falk Nimmerjahn; Josef Mautner; Wolfgang Hillen; Hermann Bujard; Jean Feuillard
Stringent doxycycline-dependent control of gene activities using an episomal one-vector system.
Nucleic acids research 2005;33(16):e137.
2004: Christel Krueger; Klaus Pfleiderer; Wolfgang Hillen; Christian Berens
Tetracycline derivatives: alternative effectors for Tet transregulators.
BioTechniques 2004;37(4):546, 548, 550.
2004: Maria A Schumacher; Gregory S Allen; Marco Diel; Gerald Seidel; Wolfgang Hillen; Richard G Brennan
Structural basis for allosteric control of the transcription regulator CcpA by the phosphoprotein HPr-Ser46-P.
Cell 2004;118(6):731-41.
2004: Lars Drueppel; Klaus Pfleiderer; Andreas Schmidt; Wolfgang Hillen; Christian Berens
A short autonomous repression motif is located within the N-terminal domain of CTCF.
FEBS letters 2004;572(1-3):154-8.
2004: Oliver Scholz; Eva-Maria Henssler; Johannes Bail; Peter Schubert; Joanna Bogdanska-Urbaniak; Sabine Sopp; Marco Reich; Stefanie Wisshak; Martin Köstner; Ralph Bertram; Wolfgang Hillen
Activity reversal of Tet repressor caused by single amino acid exchanges.
Molecular microbiology 2004;53(3):777-89.
2004: Eva-Maria Henssler; Oliver Scholz; Susanne Lochner; Peter Gmeiner; Wolfgang Hillen
Structure-based design of Tet repressor to optimize a new inducer specificity.
Biochemistry 2004;43(29):9512-8.
2004: Annette Kamionka; Miriam Sehnal; Oliver Scholz; Wolfgang Hillen
Independent regulation of two genes in Escherichia coli by tetracyclines and Tet repressor variants.
Journal of bacteriology 2004;186(13):4399-401.
2004: Peter Schubert; Klaus Pfleiderer; Wolfgang Hillen
Tet repressor residues indirectly recognizing anhydrotetracycline.
European journal of biochemistry / FEBS 2004;271(11):2144-52.
2004: Christel Krueger; Andreas Schmidt; Christina Danke; Wolfgang Hillen; Christian Berens
Transactivator mutants with altered effector specificity allow selective regulation of two genes by tetracycline variants.
Gene 2004;331():125-31.
2004: Maider Zabala; Lin Wang; Ruben Hernandez-Alcoceba; Wolfgang Hillen; Cheng Qian; Jesus Prieto; M Gabriela Kramer
Optimization of the Tet-on system to regulate interleukin 12 expression in the liver for the treatment of hepatic tumors.
Cancer research 2004;64(8):2799-804.
2004: Gesine Bauer; Christian Berens; Steven Projan; Wolfgang Hillen
Comparison of tetracycline and tigecycline binding to ribosomes mapped by dimethylsulphate and drug-directed Fe2+ cleavage of 16S rRNA.
The Journal of antimicrobial chemotherapy 2004;53(4):592-9.
2004: Carlos Esteban; Kerstin Mahr; Vicente Monedero; Wolfgang Hillen; Gaspar Pérez-Martínez; Fritz Titgemeyer
Complementation of a Delta ccpA mutant of Lactobacillus casei with CcpA mutants affected in the DNA- and cofactor-binding domains.
Microbiology (Reading, England) 2004;150(Pt 3):613-20.
2004: Ralph Bertram; Christine Kraft; Stefanie Wisshak; Judith Mueller; Oliver Scholz; Wolfgang Hillen
Phenotypes of combined tet repressor mutants for effector and operator recognition and allostery.
Journal of molecular microbiology and biotechnology 2004;8(2):104-10.
2004: Christian Berens; Wolfgang Hillen
Gene regulation by tetracyclines.
Genetic engineering 2004;26():255-77.
2004: Beatrix Suess; Barbara Fink; Christian Berens; Régis Stentz; Wolfgang Hillen
A theophylline responsive riboswitch based on helix slipping controls gene expression in vivo.
Nucleic acids research 2004;32(4):1610-4.
2004: Clemens F Leypold; Dan-Thomas Marian; Ciprian Roman; Siegfried Schneider; Peter Schubert; Oliver Scholz; Wolfgang Hillen; Timothy Clark; Harald Lanig
How does Mg(2+) affect the binding of anhydrotetracycline in the TetR protein?
Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology 2004;3(1):109-19.
2004: Annette Kamionka; Joanna Bogdanska-Urbaniak; Oliver Scholz; Wolfgang Hillen
Two mutations in the tetracycline repressor change the inducer anhydrotetracycline to a corepressor.
Nucleic acids research 2004;32(2):842-7.
2004: Matthias Merzbacher; Christian Detsch; Wolfgang Hillen; Jörg Stülke
Mycoplasma pneumoniae HPr kinase/phosphorylase.
European journal of biochemistry / FEBS 2004;271(2):367-74.
2003: Shiyi Yao; Xuewen Gao; Norbert Fuchsbauer; Wolfgang Hillen; Joachim Vater; Jinsheng Wang
Cloning, sequencing, and characterization of the genetic region relevant to biosynthesis of the lipopeptides iturin A and surfactin in Bacillus subtilis.
Current microbiology 2003;47(4):272-7.
2003: Henry Fechner; Xiaomin Wang; M Srour; Ulrike Siemetzki; Holger Seltmann; Andreas P Sutter; Hans Scherübl; Christos Zouboulis; Rainer Schwaab; Wolfgang Hillen; Heinz-Peter Schultheiss; Wolfgang Poller
A novel tetracycline-controlled transactivator-transrepressor system enables external control of oncolytic adenovirus replication.
Gene therapy 2003;10(19):1680-90.
2003: Christian Berens; Wolfgang Hillen
Gene regulation by tetracyclines. Constraints of resistance regulation in bacteria shape TetR for application in eukaryotes.
European journal of biochemistry / FEBS 2003;270(15):3109-21.
2003: Christel Krueger; Christian Berens; Andreas Schmidt; Dirk Schnappinger; Wolfgang Hillen
Single-chain Tet transregulators.
Nucleic acids research 2003;31(12):3050-6.
2003: Oliver Scholz; Martin Köstner; Marco Reich; Susanne Gastiger; Wolfgang Hillen
Teaching TetR to recognize a new inducer.
Journal of molecular biology 2003;329(2):217-27.
2003: Anja Knott; Yvonne Müller; Susan J Zunino; Christian Berens; Wolfgang Hillen
Conditional cell suicide using dox-dependent caspase-2 expression.
The journal of gene medicine 2003;5(4):343-54.
2003: Beatrix Suess; Shane Hanson; Christian Berens; Barbara Fink; Renée Schroeder; Wolfgang Hillen
Conditional gene expression by controlling translation with tetracycline-binding aptamers.
Nucleic acids research 2003;31(7):1853-8.
2003: Jonna K Koponen; Hanna Kankkonen; J Kannasto; Thomas Wirth; Wolfgang Hillen; H Bujard; Seppo Ylä-Herttuala
Doxycycline-regulated lentiviral vector system with a novel reverse transactivator rtTA2S-M2 shows a tight control of gene expression in vitro and in vivo.
Gene therapy 2003;10(6):459-66.
2003: Gregory S Allen; Katrin Steinhauer; Wolfgang Hillen; Jörg Stülke; Richard G Brennan
Crystal structure of HPr kinase/phosphatase from Mycoplasma pneumoniae.
Journal of molecular biology 2003;326(4):1203-17.
2002: Dan-Thomas Marian; Clemens Leypold; Michael Kunz; Siegfried Schneider; Peter Schubert; Oliver Scholz; Wolfgang Hillen
Pressure effect on the structure of the Tet repressor protein TetR(B).
Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology 2002;1(11):841-51.
2002: Valentina Salucci; Alessia Scarito; Luigi Aurisicchio; Stefania Lamartina; Giancarlo Nicolaus; Saverio Giampaoli; Odalys Gonzalez Paz; Carlo Toniatti; Hermann Bujard; Wolfgang Hillen; Gennaro Ciliberto; Fabio Palombo
Tight control of gene expression by a helper-dependent adenovirus vector carrying the rtTA2(s)-M2 tetracycline transactivator and repressor system.
Gene therapy 2002;9(21):1415-21.
2002: Katrin Steinhauer; Tanja Jepp; Wolfgang Hillen; Jörg Stülke
A novel mode of control of Mycoplasma pneumoniae HPr kinase/phosphatase activity reflects its parasitic lifestyle.
Microbiology (Reading, England) 2002;148(Pt 10):3277-84.
2002: Kerstin Mahr; Carlos Esteban; Wolfgang Hillen; Fritz Titgemeyer; Gaspar Pérez-Martínez
Cross communication between components of carbon catabolite repression of Lactobacillus casei and Bacillus megaterium.
Journal of molecular microbiology and biotechnology 2002;4(5):489-94.
2002: Fritz Titgemeyer; Wolfgang Hillen
Global control of sugar metabolism: a gram-positive solution.
Antonie van Leeuwenhoek 2002;82(1-4):59-71.
2002: K G Hanson; Katrin Steinhauer; Jonathan Reizer; Wolfgang Hillen; Jörg Stülke
HPr kinase/phosphatase of Bacillus subtilis: expression of the gene and effects of mutations on enzyme activity, growth and carbon catabolite repression.
Microbiology (Reading, England) 2002;148(Pt 6):1805-11.
2002: Lwin-Mar Aung-Hilbrich; Gerald Seidel; Andrea Wagner; Wolfgang Hillen
Quantification of the influence of HPrSer46P on CcpA-cre interaction.
Journal of molecular biology 2002;319(1):77-85.
2002: Anja Knott; Kristin Garke; S Urlinger; J Guthmann; Yvonne Müller; M Thellmann; Wolfgang Hillen
Tetracycline-dependent gene regulation: combinations of transregulators yield a variety of expression windows.
BioTechniques 2002;32(4):796, 798, 800 passim.
2002: Katrin Steinhauer; Gregory S Allen; Wolfgang Hillen; Jörg Stülke; Richard G Brennan
Crystallization, preliminary X-ray analysis and biophysical characterization of HPr kinase/phosphatase of Mycoplasma pneumoniae.
Acta crystallographica. Section D, Biological crystallography 2002;58(Pt 3):515-8.
2002: Stefania Lamartina; Giuseppe Roscilli; Cira Daniela Rinaudo; Elisabetta Sporeno; Luisa Silvi; Wolfgang Hillen; Hermann Bujard; Riccardo Cortese; Gennaro Ciliberto; Carlo Toniatti
Stringent control of gene expression in vivo by using novel doxycycline-dependent trans-activators.
Human gene therapy 2002;13(2):199-210.
2001: Luigi Aurisicchio; Hermann Bujard; Wolfgang Hillen; Riccardo Cortese; Gennaro Ciliberto; Nicola La Monica; Fabio Palombo
Regulated and prolonged expression of mIFN(alpha) in immunocompetent mice mediated by a helper-dependent adenovirus vector.
Gene therapy 2001;8(24):1817-25.
2001: M J Stebbins; S Urlinger; G Byrne; B Bello; Wolfgang Hillen; Jerry C P Yin
Tetracycline-inducible systems for Drosophila.
Proceedings of the National Academy of Sciences of the United States of America 2001;98(19):10775-80.
2001: Oliver Scholz; M Kintrup; Marco Reich; Wolfgang Hillen
Mechanism of Tet repressor induction by tetracyclines: length compensates for sequence in the alpha8-alpha9 loop.
Journal of molecular biology 2001;310(5):979-86.
2001: Peter Schubert; Dirk Schnappinger; Klaus Pfleiderer; Wolfgang Hillen
Identification of a stability determinant on the edge of the Tet repressor four-helix bundle dimerization motif.
Biochemistry 2001;40(11):3257-63.
2000: Andrea Wagner; E Küster-Schöck; Wolfgang Hillen
Sugar uptake and carbon catabolite repression in Bacillus megaterium strains with inactivated ptsHI.
Journal of molecular microbiology and biotechnology 2000;2(4):587-92.
2000: Oliver Scholz; Peter Schubert; M Kintrup; Wolfgang Hillen
Tet repressor induction without Mg2+.
Biochemistry 2000;39(35):10914-20.
2000: S Urlinger; Udo Baron; M Thellmann; Mazahir T Hasan; Hermann Bujard; Wolfgang Hillen
Exploring the sequence space for tetracycline-dependent transcriptional activators: novel mutations yield expanded range and sensitivity.
Proceedings of the National Academy of Sciences of the United States of America 2000;97(14):7963-8.
2000: Michael Kunz; M Kintrup; Wolfgang Hillen; Siegfried Schneider
Conformational changes induced in the Tet repressor protein TetR(B) upon operator or anhydrotetracycline binding as revealed by time-resolved fluorescence spectroscopy on single tryptophan mutants.
Photochemistry and photobiology 2000;72(1):35-48.
2000: B Tiebel; Kristin Garke; Wolfgang Hillen
Observing conformational and activity changes of tet repressor in vivo.
Nature structural biology 2000;7(6):479-81.
2000: S Urlinger; V Helbl; J Guthmann; E Pook; S Grimm; Wolfgang Hillen
The p65 domain from NF-kappaB is an efficient human activator in the tetracycline-regulatable gene expression system.
Gene 2000;247(1-2):103-10.
2000: B Vergani; M Kintrup; Wolfgang Hillen; Hans Lami; Etienne Piémont; Elisa Bombarda; P Alberti; Silvia Maria Doglia; M Chabbert
Backbone dynamics of Tet repressor alpha8intersectionalpha9 loop.
Biochemistry 2000;39(10):2759-68.
2000: Oliver Scholz; A Thiel; Wolfgang Hillen; Michael Niederweis
Quantitative analysis of gene expression with an improved green fluorescent protein. p6.
European journal of biochemistry / FEBS 2000;267(6):1565-70.
2000: Peter Orth; D Schnappinger; Wolfgang Hillen; Wolfram Saenger; W Hinrichs
Structural basis of gene regulation by the tetracycline inducible Tet repressor-operator system.
Nature structural biology 2000;7(3):215-9.
2000: M Kintrup; Peter Schubert; Michael Kunz; M Chabbert; P Alberti; Elisa Bombarda; Siegfried Schneider; Wolfgang Hillen
Trp scanning analysis of Tet repressor reveals conformational changes associated with operator and anhydrotetracycline binding.
European journal of biochemistry / FEBS 2000;267(3):821-9.
2000: Jörg Stülke; Wolfgang Hillen
Regulation of carbon catabolism in Bacillus species.
Annual review of microbiology 2000;54():849-80.
2000: Jan Tebbe; Peter Orth; E K Küster-Schöck; Wolfgang Hillen; Wolfram Saenger; W Hinrichs
Crystallization and preliminary X-ray analyses of catabolite control protein A, free and in complex with its DNA-binding site.
Acta crystallographica. Section D, Biological crystallography 2000;56(Pt 1):67-9.
2000: A Sigler; Peter Schubert; Wolfgang Hillen; Michael Niederweis
Permeation of tetracyclines through membranes of liposomes and Escherichia coli.
European journal of biochemistry / FEBS 2000;267(2):527-34.
2000: Kerstin Mahr; Wolfgang Hillen; Fritz Titgemeyer
Carbon catabolite repression in Lactobacillus pentosus: analysis of the ccpA region.
Applied and environmental microbiology 2000;66(1):277-83.
1999: E Küster-Schöck; Andrea Wagner; U Völker; Wolfgang Hillen
Mutations in catabolite control protein CcpA showing glucose-independent regulation in Bacillus megaterium.
Journal of bacteriology 1999;181(24):7634-8.
1999: P L Trelstad; P Purdhani; W Geissdörfer; Wolfgang Hillen; Jay D Keasling
Polyphosphate kinase of Acinetobacter sp. strain ADP1: purification and characterization of the enzyme and its role during changes in extracellular phosphate levels.
Applied and environmental microbiology 1999;65(9):3780-6.
1999: B Tiebel; Nicole Radzwill; Lwin-Mar Aung-Hilbrich; V Helbl; Heinz-Jürgen Steinhoff; Wolfgang Hillen
Domain motions accompanying Tet repressor induction defined by changes of interspin distances at selectively labeled sites.
Journal of molecular biology 1999;290(1):229-40.
1999: Walter Geissdörfer; R G Kok; A Ratajczak; K J Hellingwerf; Wolfgang Hillen
The genes rubA and rubB for alkane degradation in Acinetobacter sp. strain ADP1 are in an operon with estB, encoding an esterase, and oxyR.
Journal of bacteriology 1999;181(14):4292-8.
1999: E Küster; T Hilbich; Michael K Dahl; Wolfgang Hillen
Mutations in catabolite control protein CcpA separating growth effects from catabolite repression.
Journal of bacteriology 1999;181(13):4125-8.
1999: Stuart B Levy; Laura M McMurry; Teresa M Barbosa; V Burdett; P Courvalin; Wolfgang Hillen; M C Roberts; Julian I Rood; D E Taylor
Nomenclature for new tetracycline resistance determinants.
Antimicrobial agents and chemotherapy 1999;43(6):1523-4.
1999: Jörg Stülke; Wolfgang Hillen
Carbon catabolite repression in bacteria.
Current opinion in microbiology 1999;2(2):195-201.
1999: Dirk Schnappinger; Peter Schubert; Christian Berens; Klaus Pfleiderer; Wolfgang Hillen
Solvent-exposed residues in the Tet repressor (TetR) four-helix bundle contribute to subunit recognition and dimer stability.
The Journal of biological chemistry 1999;274(10):6405-10.
1999: Udo Baron; D Schnappinger; V Helbl; Manfred Gossen; Wolfgang Hillen; Hermann Bujard
Generation of conditional mutants in higher eukaryotes by switching between the expression of two genes.
Proceedings of the National Academy of Sciences of the United States of America 1999;96(3):1013-8.
1999: Peter Orth; D Schnappinger; P E Sum; G A Ellestad; Wolfgang Hillen; Wolfram Saenger; W Hinrichs
Crystal structure of the tet repressor in complex with a novel tetracycline, 9-(N,N-dimethylglycylamido)- 6-demethyl-6-deoxy-tetracycline.
Journal of molecular biology 1999;285(2):455-61.
1999: K Forster; V Helbl; Thomas Lederer; S Urlinger; N Wittenburg; Wolfgang Hillen
Tetracycline-inducible expression systems with reduced basal activity in mammalian cells.
Nucleic acids research 1999;27(2):708-10.
1998: E Pook; S Grimm; A Bonin; T Winkler; Wolfgang Hillen
Affinities of mAbs to Tet repressor complexed with operator or tetracycline suggest conformational changes associated with induction.
European journal of biochemistry / FEBS 1998;258(3):915-22.
1998: A Kraus; E Küster; Andrea Wagner; K Hoffmann; Wolfgang Hillen
Identification of a co-repressor binding site in catabolite control protein CcpA.
Molecular microbiology 1998;30(5):955-63.
1998: Jörg Stülke; Wolfgang Hillen
Coupling physiology and gene regulation in bacteria: the phosphotransferase sugar uptake system delivers the signals.
Die Naturwissenschaften 1998;85(12):583-92.
1998: A Ratajczak; Walter Geissdörfer; Wolfgang Hillen
Expression of alkane hydroxylase from Acinetobacter sp. Strain ADP1 is induced by a broad range of n-alkanes and requires the transcriptional activator AlkR.
Journal of bacteriology 1998;180(22):5822-7.
1998: B Tiebel; Lwin-Mar Aung-Hilbrich; Dirk Schnappinger; Wolfgang Hillen
Conformational changes necessary for gene regulation by Tet repressor assayed by reversible disulfide bond formation.
The EMBO journal 1998;17(17):5112-9.
1998: Peter Orth; F Cordes; D Schnappinger; Wolfgang Hillen; Wolfram Saenger; W Hinrichs
Conformational changes of the Tet repressor induced by tetracycline trapping.
Journal of molecular biology 1998;279(2):439-47.
1998: A Ratajczak; Walter Geissdörfer; Wolfgang Hillen
Alkane hydroxylase from Acinetobacter sp. strain ADP1 is encoded by alkM and belongs to a new family of bacterial integral-membrane hydrocarbon hydroxylases.
Applied and environmental microbiology 1998;64(4):1175-9.
1998: Jonathan Reizer; C Hoischen; Fritz Titgemeyer; C Rivolta; R Rabus; Jörg Stülke; Dimitri Karamata; Milton H Saier; Wolfgang Hillen
A novel protein kinase that controls carbon catabolite repression in bacteria.
Molecular microbiology 1998;27(6):1157-69.
1998: Christian Berens; B Streicher; Renée Schroeder; Wolfgang Hillen
Visualizing metal-ion-binding sites in group I introns by iron(II)-mediated Fenton reactions.
Chemistry & biology 1998;5(3):163-75.
1998: Walter Geissdörfer; A Ratajczak; Wolfgang Hillen
Transcription of ppk from Acinetobacter sp. strain ADP1, encoding a putative polyphosphate kinase, is induced by phosphate starvation.
Applied and environmental microbiology 1998;64(3):896-901.
1998: V Helbl; B Tiebel; Wolfgang Hillen
Stepwise selection of TetR variants recognizing tet operator 6C with high affinity and specificity.
Journal of molecular biology 1998;276(2):319-24.
1998: V Helbl; Wolfgang Hillen
Stepwise selection of TetR variants recognizing tet operator 4C with high affinity and specificity.
Journal of molecular biology 1998;276(2):313-8.
1998: F Schirmer; Wolfgang Hillen
The Acinetobacter calcoaceticus NCIB8250 mop operon mRNA is differentially degraded, resulting in a higher level of the 3' CatA-encoding segment than of the 5' phenolhydroxylase-encoding portion.
Molecular & general genetics : MGG 1998;257(3):330-7.
1998: Markus Biburger; Christian Berens; Thomas Lederer; T Krec; Wolfgang Hillen
Intragenic suppressors of induction-deficient TetR mutants: localization and potential mechanism of action.
Journal of bacteriology 1998;180(3):737-41.
1998: Dirk Schnappinger; Peter Schubert; Klaus Pfleiderer; Wolfgang Hillen
Determinants of protein-protein recognition by four helix bundles: changing the dimerization specificity of Tet repressor.
The EMBO journal 1998;17(2):535-43.
1997: C Späth; A Kraus; Wolfgang Hillen
Contribution of glucose kinase to glucose repression of xylose utilization in Bacillus megaterium.
Journal of bacteriology 1997;179(23):7603-5.
1997: BE Jones; V Dossonnet; E Küster; Wolfgang Hillen; Josef Deutscher; Rachel E Klevit
Binding of the catabolite repressor protein CcpA to its DNA target is regulated by phosphorylation of its corepressor HPr.
The Journal of biological chemistry 1997;272(42):26530-5.
1997: P Alberti; Elisa Bombarda; M Kintrup; Wolfgang Hillen; Hans Lami; Etienne Piémont; Silvia Maria Doglia; M Chabbert
Structural investigation of Tet repressor loop 154-167: a time-resolved fluorescence study of three single Trp mutants.
Archives of biochemistry and biophysics 1997;346(2):230-40.
1997: A Kraus; Wolfgang Hillen
Analysis of CcpA mutations defective in carbon catabolite repression in Bacillus megaterium.
FEMS microbiology letters 1997;153(1):221-6.
1997: Stephan Parche; Walter Geissdörfer; Wolfgang Hillen
Identification and characterization of xcpR encoding a subunit of the general secretory pathway necessary for dodecane degradation in Acinetobacter calcoaceticus ADP1.
Journal of bacteriology 1997;179(14):4631-4.
1997: H Backes; Christian Berens; V Helbl; S Walter; F X Schmid; Wolfgang Hillen
Combinations of the alpha-helix-turn-alpha-helix motif of TetR with respective residues from LacI or 434Cro: DNA recognition, inducer binding, and urea-dependent denaturation.
Biochemistry 1997;36(18):5311-22.
1997: D Schmiedel; P S Vary; L Jablonski; Wolfgang Hillen
Plasmids for efficient single-copy gene cloning into gdh2 and trpC of Bacillus megaterium DSM319 and QM B1551.
Applied microbiology and biotechnology 1997;47(5):543-6.
1997: P Silvi Antonini; Wolfgang Hillen; N Ettner; W Hinrichs; P Fantucci; Silvia Maria Doglia; J A Bousquet; M Chabbert
Molecular mechanics analysis of Tet repressor TRP-43 fluorescence.
Biophysical journal 1997;72(4):1800-11.
1997: Christian Berens; Dirk Schnappinger; Wolfgang Hillen
The role of the variable region in Tet repressor for inducibility by tetracycline.
The Journal of biological chemistry 1997;272(11):6936-42.
1997: R Gösseringer; E Küster; A Galinier; Josef Deutscher; Wolfgang Hillen
Cooperative and non-cooperative DNA binding modes of catabolite control protein CcpA from Bacillus megaterium result from sensing two different signals.
Journal of molecular biology 1997;266(4):665-76.
1997: D Schmiedel; M Kintrup; E Küster; Wolfgang Hillen
Regulation of expression, genetic organization and substrate specificity of xylose uptake in Bacillus megaterium.
Molecular microbiology 1997;23(5):1053-62.
1997: Walter Geissdörfer; A Ratajczak; Wolfgang Hillen
Nucleotide sequence of a putative periplasmic Mn superoxide dismutase from Acinetobacter calcoaceticus ADP1.
Gene 1997;186(2):305-8.
1997: F Schirmer; Sabine Ehrt; Wolfgang Hillen
Expression, inducer spectrum, domain structure, and function of MopR, the regulator of phenol degradation in Acinetobacter calcoaceticus NCIB8250.
Journal of bacteriology 1997;179(4):1329-36.
1996: Jonathan Reizer; U Bergstedt; Anne Galinier; E Küster; Milton H Saier; Wolfgang Hillen; M Steinmetz; Josef Deutscher
Catabolite repression resistance of gnt operon expression in Bacillus subtilis conferred by mutation of His-15, the site of phosphoenolpyruvate-dependent phosphorylation of the phosphocarrier protein HPr.
Journal of bacteriology 1996;178(18):5480-6.
1996: N Ettner; G Müller; Christian Berens; H Backes; Dirk Schnappinger; T Schreppel; Klaus Pfleiderer; Wolfgang Hillen
Fast large-scale purification of tetracycline repressor variants from overproducing Escherichia coli strains.
Journal of chromatography. A 1996;742(1-2):95-105.
1996: Thomas Lederer; M Kintrup; M Takahashi; P E Sum; G A Ellestad; Wolfgang Hillen
Tetracycline analogs affecting binding to Tn10-Encoded Tet repressor trigger the same mechanism of induction.
Biochemistry 1996;35(23):7439-46.
1996: E Küster; E J Luesink; WILLEM DE VOS; Wolfgang Hillen
Immunological crossreactivity to the catabolite control protein CcpA Bacillus megaterium is found in many gram-positive bacteria.
FEMS microbiology letters 1996;139(2-3):109-15.
1996: Dirk Schnappinger; Wolfgang Hillen
Tetracyclines: antibiotic action, uptake, and resistance mechanisms.
Archives of microbiology 1996;165(6):359-69.
1996: D Schmiedel; Wolfgang Hillen
Contributions of XylR CcpA and cre to diauxic growth of Bacillus megaterium and to xylose isomerase expression in the presence of glucose and xylose.
Molecular & general genetics : MGG 1996;250(3):259-66.
1995: Thomas Lederer; M Takahashi; Wolfgang Hillen
Thermodynamic analysis of tetracycline-mediated induction of Tet repressor by a quantitative methylation protection assay.
Analytical biochemistry 1995;232(2):190-6.
1995: Christian Berens; Klaus Pfleiderer; V Helbl; Wolfgang Hillen
Deletion mutagenesis of Tn 10 Tet repressor--localization of regions important for dimerization and inducibility in vivo.
Molecular microbiology 1995;18(3):437-48.
1995: C Peviani; Wolfgang Hillen; N Ettner; H Lami; Silvia Maria Doglia; Etienne Piémont; C Ellouze; M Chabbert
Spectroscopic investigation of Tet repressor tryptophan-43 upon specific and nonspecific DNA binding.
Biochemistry 1995;34(40):13007-15.
1995: Sabine Ehrt; F Schirmer; Wolfgang Hillen
Genetic organization, nucleotide sequence and regulation of expression of genes encoding phenol hydroxylase and catechol 1,2-dioxygenase in Acinetobacter calcoaceticus NCIB8250.
Molecular microbiology 1995;18(1):13-20.
1995: Michael K Dahl; D Schmiedel; Wolfgang Hillen
Glucose and glucose-6-phosphate interaction with Xyl repressor proteins from Bacillus spp. may contribute to regulation of xylose utilization.
Journal of bacteriology 1995;177(19):5467-72.
1995: G Müller; B Hecht; V Helbl; W Hinrichs; Wolfram Saenger; Wolfgang Hillen
Characterization of non-inducible Tet repressor mutants suggests conformational changes necessary for induction.
Nature structural biology 1995;2(8):693-703.
1995: Manfred Gossen; S Freundlieb; G Bender; G Müller; Wolfgang Hillen; Hermann Bujard
Transcriptional activation by tetracyclines in mammalian cells.
Science (New York, N.Y.) 1995;268(5218):1766-9.
1995: Tom Ramseier; Jonathan Reizer; E Küster; Wolfgang Hillen; Milton H Saier
In vitro binding of the CcpA protein of Bacillus megaterium to cis-acting catabolite responsive elements (CREs) of gram-positive bacteria.
FEMS microbiology letters 1995;129(2-3):207-13.
1995: Dirk Schnappinger; Walter Geissdörfer; C Sizemore; Wolfgang Hillen
Extracellular expression of native human anti-lysozyme fragments in Staphylococcus carnosus.
FEMS microbiology letters 1995;129(2-3):121-7.
1995: Walter Geissdörfer; S C Frosch; G Haspel; Sabine Ehrt; Wolfgang Hillen
Two genes encoding proteins with similarities to rubredoxin and rubredoxin reductase are required for conversion of dodecane to lauric acid in Acinetobacter calcoaceticus ADP1.
Microbiology (Reading, England) 1995;141 ( Pt 6)():1425-32.
1995: C J Hueck; A Kraus; D Schmiedel; Wolfgang Hillen
Cloning, expression and functional analyses of the catabolite control protein CcpA from Bacillus megaterium.
Molecular microbiology 1995;16(5):855-64.
1995: H J Kim; C Gatz; Wolfgang Hillen; T R Jones
Tetracycline repressor-regulated gene repression in recombinant human cytomegalovirus.
Journal of virology 1995;69(4):2565-73.
1995: Caroline Kisker; W Hinrichs; K Tovar; Wolfgang Hillen; Wolfram Saenger
The complex formed between Tet repressor and tetracycline-Mg2+ reveals mechanism of antibiotic resistance.
Journal of molecular biology 1995;247(2):260-80.
1995: Josef Deutscher; E Küster; U Bergstedt; Véronique Charrier; Wolfgang Hillen
Protein kinase-dependent HPr/CcpA interaction links glycolytic activity to carbon catabolite repression in gram-positive bacteria.
Molecular microbiology 1995;15(6):1049-53.
1995: V Helbl; Christian Berens; Wolfgang Hillen
Proximity probing of Tet repressor to tet operator by dimethylsulfate reveals protected and accessible functions for each recognized base-pair in the major groove.
Journal of molecular biology 1995;245(5):538-48.
1995: C J Hueck; Wolfgang Hillen
Catabolite repression in Bacillus subtilis: a global regulatory mechanism for the gram-positive bacteria?
Molecular microbiology 1995;15(3):395-401.
1995: N Ettner; J W Metzger; Thomas Lederer; J D Hulmes; Caroline Kisker; W Hinrichs; G A Ellestad; Wolfgang Hillen
Proximity mapping of the Tet repressor-tetracycline-Fe2+ complex by hydrogen peroxide mediated protein cleavage.
Biochemistry 1995;34(1):22-31.
1994: Michael K Dahl; J Degenkolb; Wolfgang Hillen
Transcription of the xyl operon is controlled in Bacillus subtilis by tandem overlapping operators spaced by four base-pairs.
Journal of molecular biology 1994;243(3):413-24.
1994: C J Hueck; Wolfgang Hillen; M H Saier
Analysis of a cis-active sequence mediating catabolite repression in gram-positive bacteria.
Research in microbiology 1994;145(7):503-18.
1994: A Scheler; Wolfgang Hillen
Regulation of xylose utilization in Bacillus licheniformis: Xyl repressor-xyl-operator interaction studied by DNA modification protection and interference.
Molecular microbiology 1994;13(3):505-12.
1994: Sabine Ehrt; L N Ornston; Wolfgang Hillen
RpoN (sigma 54) is required for conversion of phenol to catechol in Acinetobacter calcoaceticus.
Journal of bacteriology 1994;176(12):3493-9.
1994: C Hueck; A Kraus; Wolfgang Hillen
Sequences of ccpA and two downstream Bacillus megaterium genes with homology to the motAB operon from Bacillus subtilis.
Gene 1994;143(1):147-8.
1994: M Biburger; Michael Niederweis; Wolfgang Hillen
Oligo[d(C).(G)] runs exhibit a helical repeat of 11.1 bp in solution and cause slight DNA curvature when properly phased.
Nucleic acids research 1994;22(9):1562-6.
1994: W Hinrichs; Caroline Kisker; M Düvel; A Müller; K Tovar; Wolfgang Hillen; Wolfram Saenger
Structure of the Tet repressor-tetracycline complex and regulation of antibiotic resistance.
Science (New York, N.Y.) 1994;264(5157):418-20.
1994: Sabine Ehrt; Wolfgang Hillen
UDP-N-acetylglucosamine 1-carboxyvinyl-transferase from Acinetobacter calcoaceticus.
FEMS microbiology letters 1994;117(2):137-42.
1994: Michael Niederweis; Thomas Lederer; Wolfgang Hillen
Matrix effects suggest an important influence of DNA-polyacrylamide interactions on the electrophoretic mobility of DNA.
The Journal of biological chemistry 1994;269(13):10156-62.
1994: P Weinmann; Manfred Gossen; Wolfgang Hillen; Hermann Bujard; C Gatz
A chimeric transactivator allows tetracycline-responsive gene expression in whole plants.
The Plant journal : for cell and molecular biology 1994;5(4):559-69.
1994: A Kraus; C Hueck; D Gärtner; Wolfgang Hillen
Catabolite repression of the Bacillus subtilis xyl operon involves a cis element functional in the context of an unrelated sequence, and glucose exerts additional xylR-dependent repression.
Journal of bacteriology 1994;176(6):1738-45.
1994: Wolfgang Hillen; Christian Berens
Mechanisms underlying expression of Tn10 encoded tetracycline resistance.
Annual review of microbiology 1994;48():345-69.
1993: Michael Niederweis; Wolfgang Hillen
Electrophoretic analysis of protein-induced DNA bending and twist changes.
Electrophoresis 1993;14(8):693-8.
1993: C Kauder; R Allmansberger; D Gärtner; D Schmiedel; Wolfgang Hillen
An operator binding-negative mutation of Xyl repressor from Bacillus subtilis is trans dominant in Bacillus megaterium.
FEMS microbiology letters 1993;109(1):81-4.
1993: C Sizemore; Walter Geissdörfer; Wolfgang Hillen
Using fusions with luxAB from Vibrio harveyi MAV to quantify induction and catabolite repression of the xyl operon in Staphylococcus carnosus TM300.
FEMS microbiology letters 1993;107(2-3):303-6.
1993: B Hecht; G Müller; Wolfgang Hillen
Noninducible Tet repressor mutations map from the operator binding motif to the C terminus.
Journal of bacteriology 1993;175(4):1206-10.
1992: Michael Niederweis; Thomas Lederer; Wolfgang Hillen
An accurate method for determining the helical repeat of DNA in solution reveals differences to the crystal structures of two B-DNA decamers.
Journal of molecular biology 1992;228(2):322-6.
1992: Ralf Baumeister; G Müller; B Hecht; Wolfgang Hillen
Functional roles of amino acid residues involved in forming the alpha-helix-turn-alpha-helix operator DNA binding motif of Tet repressor from Tn10.
Proteins 1992;14(2):168-77.
1992: Ralf Baumeister; V Helbl; Wolfgang Hillen
Contacts between Tet repressor and tet operator revealed by new recognition specificities of single amino acid replacement mutants.
Journal of molecular biology 1992;226(4):1257-70.
1992: T Rygus; Wolfgang Hillen
Catabolite repression of the xyl operon in Bacillus megaterium.
Journal of bacteriology 1992;174(9):3049-55.
1992: C Sizemore; B Wieland; F Götz; Wolfgang Hillen
Regulation of Staphylococcus xylosus xylose utilization genes at the molecular level.
Journal of bacteriology 1992;174(9):3042-8.
1992: U Gülland; Wolfgang Hillen
The Tn10-encoded tetR mRNA has heterogeneous 5' ends in vivo and in vitro.
Gene 1992;114(1):97-101.
1992: D Gärtner; J Degenkolb; J A Ripperger; R Allmansberger; Wolfgang Hillen
Regulation of the Bacillus subtilis W23 xylose utilization operon: interaction of the Xyl repressor with the xyl operator and the inducer xylose.
Molecular & general genetics : MGG 1992;232(3):415-22.
1992: Theo Dingermann; U Frank-Stoll; H Werner; A Wissmann; Wolfgang Hillen; Michel Jacquet; Rolf Marschalek
RNA polymerase III catalysed transcription can be regulated in Saccharomyces cerevisiae by the bacterial tetracycline repressor-operator system.
The EMBO journal 1992;11(4):1487-92.
1992: P Flache; Ralf Baumeister; Wolfgang Hillen
The Tn10-encoded tetracycline resistance mRNA contains a translational silencer in the 5' nontranslated region.
Journal of bacteriology 1992;174(8):2478-84.
1992: M Chabbert; Wolfgang Hillen; D Hansen; M Takahashi; J A Bousquet
Structural analysis of the operator binding domain of Tn10-encoded Tet repressor: a time-resolved fluorescence and anisotropy study.
Biochemistry 1992;31(7):1951-60.
1992: Christian Berens; L Altschmied; Wolfgang Hillen
The role of the N terminus in Tet repressor for tet operator binding determined by a mutational analysis.
The Journal of biological chemistry 1992;267(3):1945-52.
1991: M Takahashi; J Degenkolb; Wolfgang Hillen
Determination of the equilibrium association constant between Tet repressor and tetracycline at limiting Mg2+ concentrations: a generally applicable method for effector-dependent high-affinity complexes.
Analytical biochemistry 1991;199(2):197-202.
1991: A Wissmann; Ralf Baumeister; G Müller; B Hecht; V Helbl; Klaus Pfleiderer; Wolfgang Hillen
Amino acids determining operator binding specificity in the helix-turn-helix motif of Tn10 Tet repressor.
The EMBO journal 1991;10(13):4145-52.
1991: S Jacob; R Allmansberger; D Gärtner; Wolfgang Hillen
Catabolite repression of the operon for xylose utilization from Bacillus subtilis W23 is mediated at the level of transcription and depends on a cis site in the xylA reading frame.
Molecular & general genetics : MGG 1991;229(2):189-96.
1991: Ralf Baumeister; P Flache; ojar melefors; Alexander von Gabain; Wolfgang Hillen
Lack of a 5' non-coding region in Tn1721 encoded tetR mRNA is associated with a low efficiency of translation and a short half-life in Escherichia coli.
Nucleic acids research 1991;19(17):4595-600.
1991: J Degenkolb; M Takahashi; G A Ellestad; Wolfgang Hillen
Structural requirements of tetracycline-Tet repressor interaction: determination of equilibrium binding constants for tetracycline analogs with the Tet repressor.
Antimicrobial agents and chemotherapy 1991;35(8):1591-5.
1991: T Rygus; Wolfgang Hillen
Inducible high-level expression of heterologous genes in Bacillus megaterium using the regulatory elements of the xylose-utilization operon.
Applied microbiology and biotechnology 1991;35(5):594-9.
1991: C Sizemore; Eberhard Buchner; T Rygus; C Witke; F Götz; Wolfgang Hillen
Organization, promoter analysis and transcriptional regulation of the Staphylococcus xylosus xylose utilization operon.
Molecular & general genetics : MGG 1991;227(3):377-84.
1991: A Wissmann; L V Wray; U Somaggio; Ralf Baumeister; M Geissendörfer; Wolfgang Hillen
Selection for Tn10 tet repressor binding to tet operator in Escherichia coli: isolation of temperature-sensitive mutants and combinatorial mutagenesis in the DNA binding motif.
Genetics 1991;128(2):225-32.
1991: C Kleinschmidt; K Tovar; Wolfgang Hillen
Computer simulations and experimental studies of gel mobility patterns for weak and strong non-cooperative protein binding to two targets on the same DNA: application to binding of tet repressor variants to multiple and single tet operator sites.
Nucleic acids research 1991;19(5):1021-8.
1991: A Scheler; T Rygus; R Allmansberger; Wolfgang Hillen
Molecular cloning, structure, promoters and regulatory elements for transcription of the Bacillus licheniformis encoded regulon for xylose utilization.
Archives of microbiology 1991;155(6):526-34.
1991: K Tovar; Wolfgang Hillen
Large-scale preparation of DNA fragments for physical studies of protein binding.
Methods in enzymology 1991;208():54-63.
1991: A Wissmann; Wolfgang Hillen
DNA contacts probed by modification protection and interference studies.
Methods in enzymology 1991;208():365-79.
1991: T Rygus; A Scheler; R Allmansberger; Wolfgang Hillen
Molecular cloning, structure, promoters and regulatory elements for transcription of the Bacillus megaterium encoded regulon for xylose utilization.
Archives of microbiology 1991;155(6):535-42.
1991: B Surek; M Wilhelm; Wolfgang Hillen
Optimizing the promoter and ribosome binding sequence for expression of human single chain urokinase-like plasminogen activator in Escherichia coli and stabilization of the product by avoiding heat shock response.
Applied microbiology and biotechnology 1991;34(4):488-94.
1990: V Kishan; Wolfgang Hillen
Molecular cloning, nucleotide sequence, and promoter structure of the Acinetobacter calcoaceticus trpFB operon.
Journal of bacteriology 1990;172(10):6151-5.
1990: M Geissendörfer; Wolfgang Hillen
Regulated expression of heterologous genes in Bacillus subtilis using the Tn10 encoded tet regulatory elements.
Applied microbiology and biotechnology 1990;33(6):657-63.
1990: C Sizemore; A Wissmann; U Gülland; Wolfgang Hillen
Quantitative analysis of Tn10 Tet repressor binding to a complete set of tet operator mutants.
Nucleic acids research 1990;18(10):2875-80.
1990: M Hunger; R Schmucker; V Kishan; Wolfgang Hillen
Analysis and nucleotide sequence of an origin of DNA replication in Acinetobacter calcoaceticus and its use for Escherichia coli shuttle plasmids.
Gene 1990;87(1):45-51.
1990: G Haspel; M Hunger; R Schmucker; Wolfgang Hillen
Identification and nucleotide sequence of the Acinetobacter calcoaceticus encoded trpE gene.
Molecular & general genetics : MGG 1990;220(3):475-7.
1989: K Tovar; Wolfgang Hillen
Tet repressor binding induced curvature of tet operator DNA.
Nucleic acids research 1989;17(16):6515-22.
1989: Stuart B Levy; Laura M McMurry; V Burdett; P Courvalin; Wolfgang Hillen; M C Roberts; D E Taylor
Nomenclature for tetracycline resistance determinants.
Antimicrobial agents and chemotherapy 1989;33(8):1373-4.
1989: P Kreuzer; D Gärtner; R Allmansberger; Wolfgang Hillen
Identification and sequence analysis of the Bacillus subtilis W23 xylR gene and xyl operator.
Journal of bacteriology 1989;171(7):3840-5.
1989: M Takahashi; B Blazy; A Baudras; Wolfgang Hillen
Ligand-modulated binding of a gene regulatory protein to DNA. Quantitative analysis of cyclic-AMP induced binding of CRP from Escherichia coli to non-specific and specific DNA targets.
Journal of molecular biology 1989;207(4):783-96.
1989: Detlev Riesner; Gerhard Steger; R Zimmat; R A Owens; M Wagenhöfer; Wolfgang Hillen; S Vollbach; K Henco
Temperature-gradient gel electrophoresis of nucleic acids: analysis of conformational transitions, sequence variations, and protein-nucleic acid interactions.
Electrophoresis 1989;10(5-6):377-89.
1989: H Lederer; K Tovar; G Baer; R P May; Wolfgang Hillen; Hermann Heumann
The quaternary structure of Tet repressors bound to the Tn10-encoded tet gene control region determined by neutron solution scattering.
The EMBO journal 1989;8(4):1257-63.
1988: K Tovar; A Ernst; Wolfgang Hillen
Identification and nucleotide sequence of the class E tet regulatory elements and operator and inducer binding of the encoded purified Tet repressor.
Molecular & general genetics : MGG 1988;215(1):76-80.
1988: M Wagenhöfer; D Hansen; Wolfgang Hillen
Thermal denaturation of engineered tet repressor proteins and their complexes with tet operator and tetracycline studied by temperature gradient gel electrophoresis.
Analytical biochemistry 1988;175(2):422-32.
1988: L Altschmied; Ralf Baumeister; Klaus Pfleiderer; Wolfgang Hillen
A threonine to alanine exchange at position 40 of Tet repressor alters the recognition of the sixth base pair of tet operator from GC to AT.
The EMBO journal 1988;7(12):4011-7.
1988: C Heuer; Wolfgang Hillen
Tet repressor-tet operator contacts probed by operator DNA-modification interference studies.
Journal of molecular biology 1988;202(3):407-15.
1988: A Wissmann; I Meier; Wolfgang Hillen
Saturation mutagenesis of the Tn10-encoded tet operator O1. Identification of base-pairs involved in Tet repressor recognition.
Journal of molecular biology 1988;202(3):397-406.
1988: D Gärtner; M Geissendörfer; Wolfgang Hillen
Expression of the Bacillus subtilis xyl operon is repressed at the level of transcription and is induced by xylose.
Journal of bacteriology 1988;170(7):3102-9.
1988: C Kleinschmidt; K Tovar; Wolfgang Hillen; Dietmar Porschke
Dynamics of repressor-operator recognition: the Tn10-encoded tetracycline resistance control.
Biochemistry 1988;27(4):1094-104.
1988: I Meier; L V Wray; Wolfgang Hillen
Differential regulation of the Tn10-encoded tetracycline resistance genes tetA and tetR by the tandem tet operators O1 and O2.
The EMBO journal 1988;7(2):567-72.
1987: D Hansen; L Altschmied; Wolfgang Hillen
Engineered Tet repressor mutants with single tryptophan residues as fluorescent probes. Solvent accessibilities of DNA and inducer binding sites and interaction with tetracycline.
The Journal of biological chemistry 1987;262(29):14030-5.
1987: D Hansen; Wolfgang Hillen
Tryptophan in alpha-helix 3 of Tet repressor forms a sequence-specific contact with tet operator in solution.
The Journal of biological chemistry 1987;262(25):12269-74.
1987: Wolfgang Hillen; U Schiebel; T Zaengel
Imaging performance of a digital storage phosphor system.
Medical physics 1987;14(5):744-51.
1987: C Heuer; Robert Hickman; Michael Curiale; Wolfgang Hillen; S B Levy
Constitutive expression of tetracycline resistance mediated by a Tn10-like element in Haemophilus parainfluenzae results from a mutation in the repressor gene.
Journal of bacteriology 1987;169(3):990-4.
1986: C Gatz; J Altschmied; Wolfgang Hillen
Cloning and expression of the Acinetobacter calcoaceticus mutarotase gene in Escherichia coli.
Journal of bacteriology 1986;168(1):31-9.
1986: G Klock; Wolfgang Hillen
Expression, purification and operator binding of the transposon Tn1721-encoded Tet repressor.
Journal of molecular biology 1986;189(4):633-41.
1986: A Wissmann; I Meier; L V Wray; M Geissendörfer; Wolfgang Hillen
Tn10 tet operator mutations affecting Tet repressor recognition.
Nucleic acids research 1986;14(10):4253-66.
1986: C Gatz; Wolfgang Hillen
Acinetobacter calcoaceticus encoded mutarotase: nucleotide sequence analysis of the gene and characterization of its secretion in Escherichia coli.
Nucleic acids research 1986;14(10):4309-23.
1986: M Takahashi; L Altschmied; Wolfgang Hillen
Kinetic and equilibrium characterization of the Tet repressor-tetracycline complex by fluorescence measurements. Evidence for divalent metal ion requirement and energy transfer.
Journal of molecular biology 1986;187(3):341-8.
1985: K Schollmeier; D Gärtner; Wolfgang Hillen
A bidirectionally active signal for termination of transcription is located between tetA and orfL on transposon Tn10.
Nucleic acids research 1985;13(12):4227-37.
1985: G Klock; B Unger; C Gatz; Wolfgang Hillen; J Altenbuchner; K Schmid; R Schmitt
Heterologous repressor-operator recognition among four classes of tetracycline resistance determinants.
Journal of bacteriology 1985;161(1):326-32.
1984: H E Parge; M Schneider; V Hahn; W Saenger; L Altschmied; Wolfgang Hillen
Crystallization of and preliminary X-ray diffraction data for TET-repressor and the TET-repressor-tetracycline complex.
Journal of molecular biology 1984;180(4):1189-91.
1984: D Porschke; Wolfgang Hillen; M Takahashi
The change of DNA structure by specific binding of the cAMP receptor protein from rotation diffusion and dichroism measurements.
The EMBO journal 1984;3(12):2873-8.
1984: B Unger; J Becker; Wolfgang Hillen
Nucleotide sequence of the gene, protein purification and characterization of the pSC101-encoded tetracycline resistance-gene-repressor.
Gene 1984;31(1-3):103-8.
1984: K Schollmeier; Wolfgang Hillen
Transposon Tn10 contains two structural genes with opposite polarity between tetA and IS10R.
Journal of bacteriology 1984;160(2):499-503.
1984: B Unger; G Klock; Wolfgang Hillen
Nucleotide sequence of the repressor gene of the RA1 tetracycline resistance determinant: structural and functional comparison with three related Tet repressor genes.
Nucleic acids research 1984;12(20):7693-703.
1984: B Brosius; Detlev Riesner; Wolfgang Hillen
Interaction of nucleic acids with lipid membranes.
Journal of biomolecular structure & dynamics 1984;1(6):1535-41.
1984: R Oehmichen; G Klock; L Altschmied; Wolfgang Hillen
Construction of an E. coli strain overproducing the Tn10-encoded TET repressor and its use for large scale purification.
The EMBO journal 1984;3(3):539-43.
1984: L Altschmied; Wolfgang Hillen
TET repressor.tet operator complex formation induces conformational changes in the tet operator DNA.
Nucleic acids research 1984;12(4):2171-80.
1984: Wolfgang Hillen; K Schollmeier; C Gatz
Control of expression of the Tn10-encoded tetracycline resistance operon. II. Interaction of RNA polymerase and TET repressor with the tet operon regulatory region.
Journal of molecular biology 1984;172(2):185-201.
1983: Wolfgang Hillen; C Gatz; L Altschmied; K Schollmeier; I Meier
Control of expression of the Tn10-encoded tetracycline resistance genes. Equilibrium and kinetic investigation of the regulatory reactions.
Journal of molecular biology 1983;169(3):707-21.
1983: M Takahashi; B Blazy; A Baudras; Wolfgang Hillen
On the origin of selectivity in recognition by cyclic adenosine 3',5'-monophosphate receptor protein of its specific binding site of the lactose promoter region.
Journal of molecular biology 1983;167(4):895-9.
1983: Wolfgang Hillen; K Schollmeier
Nucleotide sequence of the Tn10 encoded tetracycline resistance gene.
Nucleic acids research 1983;11(2):525-39.
1983: B Unger; G M Clore; A M Gronenborn; Wolfgang Hillen
Specific DNA binding of the cAMP receptor protein within the lac operon stabilizes double-stranded DNA in the presence of cAMP.
The EMBO journal 1983;2(2):289-93.
1983: Robert D Wells; R Brennan; K A Chapman; T C Goodman; P A Hart; Wolfgang Hillen; Doug R Kellogg; M W Kilpatrick; R D Klein; J Klysik; P F Lambert; J E Larson; J J Miglietta; S K Neuendorf; T R O'Connor; C K Singleton; S M Stirdivant; C M Veneziale; Roger M Wartell; W Zacharias
Left-handed DNA helices, supercoiling, and the B-Z junction.
Cold Spring Harbor symposia on quantitative biology 1983;47 Pt 1():77-84.
1982: Wolfgang Hillen; B Unger; G Klock
Analysis of tet operator-TET repressor complexes by thermal denaturation studies.
Nucleic acids research 1982;10(19):6085-97.
1982: S Diekmann; Wolfgang Hillen; B Morgeneyer; R D Wells; D Pörschke
Orientation relaxation of DNA restriction fragments and the internal mobility of the double helix.
Biophysical chemistry 1982;15(4):263-70.
1982: D Pörschke; N Geisler; Wolfgang Hillen
Structure of the complex between lac repressor headpiece and operator DNA from measurements of the orientation relaxation and the electric dichroism.
Nucleic acids research 1982;10(12):3791-802.
1982: Wolfgang Hillen; B Unger
Binding of four repressors to double-stranded tet operator region stabilizes it against thermal denaturation.
Nature 1982;297(5868):700-2.
1982: Wolfgang Hillen; G Klock; I Kaffenberger; L V Wray; W S Reznikoff
Purification of the TET repressor and TET operator from the transposon Tn10 and characterization of their interaction.
The Journal of biological chemistry 1982;257(11):6605-13.
1982: S Diekmann; Wolfgang Hillen; M Jung; R D Wells; D Pörschke
Electric properties and structure of DNA-restriction fragments from measurements of the electric dichroism.
Biophysical chemistry 1982;15(2):157-67.
1982: Wolfgang Hillen; B Unger
Correlation of thermodynamic and genetic properties in the Tn10 encoded TET gene control region.
Nucleic acids research 1982;10(8):2685-700.
1982: Roger M Wartell; J Klysik; Wolfgang Hillen; Robert D Wells
Junction between Z and B conformations in a DNA restriction fragment: evaluation by Raman spectroscopy.
Proceedings of the National Academy of Sciences of the United States of America 1982;79(8):2549-53.
1981: T C Goodman; Stephen Hardies; C Cortez; Wolfgang Hillen
Computer programs to assist in high resolution thermal denaturation and circular dichroism studies on nucleic acids.
Nucleic acids research 1981;9(24):7033-40.
1981: Wolfgang Hillen; T C Goodman; R D Wells
Circular dichroism spectra of twelve short DNA restriction fragments of known sequence: a comparison of measured and calculated spectra.
Nucleic acids research 1981;9(13):3029-45.
1981: T A Early; D R Kearns; Wolfgang Hillen; R D Wells
A 300-MHz proton nuclear magnetic resonance investigation of deoxyribonucleic acid restriction fragments: dynamic properties.
Biochemistry 1981;20(13):3764-9.
1981: T A Early; D R Kearns; Wolfgang Hillen; R D Wells
A 300- and 600-MHz proton nuclear magnetic resonance investigation of a 12 base pair deoxyribonucleic acid restriction fragment: relaxation behavior of the low-field resonances in water.
Biochemistry 1981;20(13):3756-64.
1981: Wolfgang Hillen; R D Klein; R D Wells
Preparation of milligram amounts of 21 deoxyribonucleic acid restriction fragments.
Biochemistry 1981;20(13):3748-56.
1981: Wolfgang Hillen; T C Goodman; A S Benight; Roger M Wartell; R D Wells
High resolution experimental and theoretical thermal denaturation studies on small overlapping restriction fragments containing the Escherichia coli lactose genetic control region.
The Journal of biological chemistry 1981;256(6):2761-6.
1981: Wolfgang Hillen; T C Goodman; R D Wells
Salt dependence and thermodynamic interpretation of the thermal denaturation of small DNA restriction fragments.
Nucleic acids research 1981;9(2):415-36.
1980: T A Early; D R Kearns; Wolfgang Hillen; R D Wells
A 300 MHz and 600 MHz proton NMR study of a 12 base pair restriction fragment: investigation of structure by relaxation measurements.
Nucleic acids research 1980;8(23):5795-812.
1980: Wolfgang Hillen; R D Wells
Circular dichroism studies of the B goes to A conformational transition in seven small DNA restriction fragments containing the Escherichia coli lactose control region.
Nucleic acids research 1980;8(22):5427-44.
1980: Robert D Wells; T C Goodman; Wolfgang Hillen; Glenn T Horn; R D Klein; J E Larson; U R Müller; S K Neuendorf; N Panayotatos; S M Stirdivant
DNA structure and gene regulation.
Progress in nucleic acid research and molecular biology 1980;24():167-267.
1979: Stephen Hardies; Wolfgang Hillen; T C Goodman; R D Wells
High resolution thermal denaturation analyses of small sequenced DNA restriction fragments containing Escherichia coli lactose genetic control loci.
The Journal of biological chemistry 1979;254(20):10128-34.
1979: Wolfgang Hillen; H G Gassen
Physical and coding properties of poly(5-methoxyuridylic) acid.
Biochimica et biophysica acta 1979;562(2):207-13.
1978: Wolfgang Hillen; E Egert; H J Lindner; H G Gassen
Crystal and molecular structure of 2-thio-5 carboxymethyluridine and its methyl ester: helix terminator nucleosides in the first position of some anticodons.
Biochemistry 1978;17(24):5314-20.
1978: Wolfgang Hillen; E Egert; H J Lindner; H G Gassen
Restriction or amplification of wobble recognition: the structure of 2-thio-5-methylaminomethyluridine and the interaction of odd uridines with the anticodon loop backbone.
FEBS letters 1978;94(2):361-4.
1978: Wolfgang Hillen; G Gassen
5-Substituents in the uridine moiety and their effect on the conformation of ApU-type dinucleoside phosphates.
Biochimica et biophysica acta 1978;518(1):7-16.
1977: E Egert; H J Lindner; Wolfgang Hillen; H G Gassen
5-Nitrouridine-monohydrate: crystal structure and conformation.
Nucleic acids research 1977;4(4):929-37.
1975: Wolfgang Hillen; H G Gassen
Physical and coding properties of poly(5-aminouridylic acid) and of 5-aminouridine-containing trinucleotides.
Biochimica et biophysica acta 1975;407(3):347-56.