Wolfgang Hillen

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Klieber Michael A; Scholz Oliver; Lochner Susanne; Gmeiner Peter; Hillen Wolfgang; Muller Yves A
Structural origins for selectivity and specificity in an engineered bacterial repressor-inducer pair.
Hunsicker Anke; Steber Markus; Mayer Günter; Meitert Johannes; Klotzsche Marcus; Blind Michael; Hillen Wolfgang; Berens Christian; Suess Beatrix
An RNA aptamer that induces transcription.
Daam Janko; Mehdaoui Kaoutar; Klotzsche Marcus; Pfleiderer Klaus; Berens Christian; Hillen Wolfgang
Functionally important residues of the Tet repressor inducing peptide TIP determined by a complete mutational analysis.

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All Publications

2009: Klieber Michael A; Scholz Oliver; Lochner Susanne; Gmeiner Peter; Hillen Wolfgang; Muller Yves A
Structural origins for selectivity and specificity in an engineered bacterial repressor-inducer pair.
The FEBS journal 2009;276(19):5610-21.
2009: Hunsicker Anke; Steber Markus; Mayer Günter; Meitert Johannes; Klotzsche Marcus; Blind Michael; Hillen Wolfgang; Berens Christian; Suess Beatrix
An RNA aptamer that induces transcription.
Chemistry & biology 2009;16(2):173-80.
2008: Daam Janko; Mehdaoui Kaoutar; Klotzsche Marcus; Pfleiderer Klaus; Berens Christian; Hillen Wolfgang
Functionally important residues of the Tet repressor inducing peptide TIP determined by a complete mutational analysis.
Gene 2008;423(2):201-6.
2008: Palm Gottfried J; Lederer Thomas; Orth Peter; Saenger Wolfram; Takahashi Masayuki; Hillen Wolfgang; Hinrichs Winfried
Specific binding of divalent metal ions to tetracycline and to the Tet repressor/tetracycline complex.
Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry 2008;13(7):1097-110.
2008: Resch Marcus; Striegl Harald; Henssler Eva Maria; Sevvana Madhumati; Egerer-Sieber Claudia; Schiltz Emile; Hillen Wolfgang; Muller Yves A
A protein functional leap: how a single mutation reverses the function of the transcription regulator TetR.
Nucleic acids research 2008;36(13):4390-401.
2007: Gandotra Sheetal; Schnappinger Dirk; Monteleone Mercedes; Hillen Wolfgang; Ehrt Sabine
In vivo gene silencing identifies the Mycobacterium tuberculosis proteasome as essential for the bacteria to persist in mice.
Nature medicine 2007;13(12):1515-20.
2007: Krueger Marcus; Scholz Oliver; Wisshak Stefanie; Hillen Wolfgang
Engineered Tet repressors with recognition specificity for the tetO-4C5G operator variant.
Gene 2007;404(1-2):93-100.
2007: Guo Xinzheng V; Monteleone Mercedes; Klotzsche Marcus; Kamionka Annette; Hillen Wolfgang; Braunstein Miriam; Ehrt Sabine; Schnappinger Dirk
Silencing Mycobacterium smegmatis by using tetracycline repressors.
Journal of bacteriology 2007;189(13):4614-23.
2007: Alper Selver M; Fischer Felix; Kuntalp Mehmet; Hillen Walter
A software tool for interactive generation, representation, and systematical storage of transfer functions for 3D medical images.
Computer methods and programs in biomedicine 2007;86(3):270-80.
2007: Schumacher Maria A; Seidel Gerald; Hillen Wolfgang; Brennan Richard G
Structural mechanism for the fine-tuning of CcpA function by the small molecule effectors glucose 6-phosphate and fructose 1,6-bisphosphate.
Journal of molecular biology 2007;368(4):1042-50.
2007: Luckner Sylvia R; Klotzsche Marcus; Berens Christian; Hillen Wolfgang; Muller Yves A
How an agonist peptide mimics the antibiotic tetracycline to induce Tet-repressor.
Journal of molecular biology 2007;368(3):780-90.
2007: Mertins Sonja; Joseph Biju; Goetz Monika; Ecke Regina; Seidel Gerald; Sprehe Mareen; Hillen Wolfgang; Goebel Werner; Müller-Altrock Stefanie
Interference of components of the phosphoenolpyruvate phosphotransferase system with the central virulence gene regulator PrfA of Listeria monocytogenes.
Journal of bacteriology 2007;189(2):473-90.
2007: Horstmann Nicola; Seidel Gerald; Aung-Hilbrich Lwin-Mar; Hillen Wolfgang
Residues His-15 and Arg-17 of HPr participate differently in catabolite signal processing via CcpA.
The Journal of biological chemistry 2007;282(2):1175-82.
2007: Klotzsche Marcus; Goeke Dagmar; Berens Christian; Hillen Wolfgang
Efficient and exclusive induction of Tet repressor by the oligopeptide Tip results from co-variation of their interaction site.
Nucleic acids research 2007;35(12):3945-52.
2007: Zhou Xue; Symons Jori; Hoppes Rieuwert; Krueger Christel; Berens Christian; Hillen Wolfgang; Berkhout Ben; Das Atze T
Improved single-chain transactivators of the Tet-On gene expression system.
BMC biotechnology 2007;7():6.
2007: Sprehe Mareen; Seidel Gerald; Diel Marco; Hillen Wolfgang
CcpA mutants with differential activities in Bacillus subtilis.
Journal of molecular microbiology and biotechnology 2007;12(1-2):96-105.
2006: Mauder Norman; Ecke Regina; Mertins Sonja; Loeffler Daniela I M; Seidel Gerald; Sprehe Mareen; Hillen Wolfgang; Goebel Werner; Müller-Altrock Stefanie
Species-specific differences in the activity of PrfA, the key regulator of listerial virulence genes.
Journal of bacteriology 2006;188(22):7941-56.
2006: Berens Christian; Lochner Susanne; Löber Stefan; Usai Igor; Schmidt Andreas; Drueppel Lars; Hillen Wolfgang; Gmeiner Peter
Subtype selective tetracycline agonists and their application for a two-stage regulatory system.
Chembiochem : a European journal of chemical biology 2006;7(9):1320-4.
2006: Schlicht Maximilian; Berens Christian; Daam Janko; Hillen Wolfgang
Random insertion of a TetR-inducing peptide tag into Escherichia coli proteins allows analysis of protein levels by induction of reporter gene expression.
Applied and environmental microbiology 2006;72(8):5637-42.
2006: Krueger Christel; Danke Christina; Pfleiderer Klaus; Schuh Wolfgang; Jäck Hans-Martin; Lochner Susanne; Gmeiner Peter; Hillen Wolfgang; Berens Christian
A gene regulation system with four distinct expression levels.
The journal of gene medicine 2006;8(8):1037-47.
2006: Köstner Martin; Schmidt Britta; Bertram Ralph; Hillen Wolfgang
Generating tetracycline-inducible auxotrophy in Escherichia coli and Salmonella enterica serovar Typhimurium by using an insertion element and a hyperactive transposase.
Applied and environmental microbiology 2006;72(7):4717-25.
2006: Bertram Ralph; Wünsche Andrea; Sprehe Mareen; Hillen Wolfgang
Regulated expression of HPrK/P does not affect carbon catabolite repression of the xyn operon and of rocG in Bacillus subtilis.
FEMS microbiology letters 2006;259(1):147-52.
2006: Müller Wolfgang; Horstmann Nicola; Hillen Wolfgang; Sticht Heinrich
The transcription regulator RbsR represents a novel interaction partner of the phosphoprotein HPr-Ser46-P in Bacillus subtilis.
The FEBS journal 2006;273(6):1251-61.
2006: Schumacher Maria A; Seidel Gerald; Hillen Wolfgang; Brennan Richard G
Phosphoprotein Crh-Ser46-P displays altered binding to CcpA to effect carbon catabolite regulation.
The Journal of biological chemistry 2006;281(10):6793-800.
2006: Goldring Chris E P; Kitteringham Neil R; Jenkins Rosalind; Lovatt Cerys A; Randle Laura E; Abdullah Azman; Owen Andrew; Liu Xiaoping; Butler Philip J; Williams Dominic P; Metcalfe Peter; Berens Christian; Hillen Wolfgang; Foster Brian; Simpson Alec; McLellan Lesley; Park B Kevin
Development of a transactivator in hepatoma cells that allows expression of phase I, phase II, and chemical defense genes.
American journal of physiology. Cell physiology 2006;290(1):C104-15.
2006: Kamionka Annette; Majewski Marius; Roth Karin; Bertram Ralph; Kraft Christine; Hillen Wolfgang
Induction of single chain tetracycline repressor requires the binding of two inducers.
Nucleic acids research 2006;34(14):3834-41.
2005: Henssler Eva-Maria; Bertram Ralph; Wisshak Stefanie; Hillen Wolfgang
Tet repressor mutants with altered effector binding and allostery.
The FEBS journal 2005;272(17):4487-96.
2005: Klotzsche Marcus; Berens Christian; Hillen Wolfgang
A peptide triggers allostery in tet repressor by binding to a unique site.
The Journal of biological chemistry 2005;280(26):24591-9.
2005: Seidel Gerald; Diel Marco; Fuchsbauer Norbert; Hillen Wolfgang
Quantitative interdependence of coeffectors, CcpA and cre in carbon catabolite regulation of Bacillus subtilis.
The FEBS journal 2005;272(10):2566-77.
2005: Knott Anja; Drueppel Lars; Beyer Thomas; Garke Kristin; Berens Christian; Herrmann Martin; Hillen Wolfgang
An optimized conditional suicide switch using doxycycline-dependent expression of human tBid.
Cancer biology & therapy 2005;4(5):532-6.
2005: Kamionka Annette; Bertram Ralph; Hillen Wolfgang
Tetracycline-dependent conditional gene knockout in Bacillus subtilis.
Applied and environmental microbiology 2005;71(2):728-33.
2005: Ameres Stefan Ludwig; Drueppel Lars; Pfleiderer Klaus; Schmidt Andreas; Hillen Wolfgang; Berens Christian
Inducible DNA-loop formation blocks transcriptional activation by an SV40 enhancer.
The EMBO journal 2005;24(2):358-67.
2005: Bertram Ralph; Köstner Martin; Müller Judith; Vazquez Ramos José; Hillen Wolfgang
Integrative elements for Bacillus subtilis yielding tetracycline-dependent growth phenotypes.
Nucleic acids research 2005;33(18):e153.
2005: Wong Ee Tsin; Kolman John L; Li Yao-Cheng; Mesner Larry D; Hillen Wolfgang; Berens Christian; Wahl Geoffrey M
Reproducible doxycycline-inducible transgene expression at specific loci generated by Cre-recombinase mediated cassette exchange.
Nucleic acids research 2005;33(17):e147.
2005: Bornkamm Georg W; Berens Christian; Kuklik-Roos Conny; Bechet Jean-Marie; Laux Gerhard; Bachl Jürgen; Korndoerfer Martin; Schlee Martin; Hölzel Michael; Malamoussi Anastassia; Chapman Rob D; Nimmerjahn Falk; Mautner Josef; Hillen Wolfgang; Bujard Hermann; Feuillard Jean
Stringent doxycycline-dependent control of gene activities using an episomal one-vector system.
Nucleic acids research 2005;33(16):e137.
2004: Krueger Christel; Pfleiderer Klaus; Hillen Wolfgang; Berens Christian
Tetracycline derivatives: alternative effectors for Tet transregulators.
BioTechniques 2004;37(4):546, 548, 550.
2004: Schumacher Maria A; Allen Gregory S; Diel Marco; Seidel Gerald; Hillen Wolfgang; Brennan Richard G
Structural basis for allosteric control of the transcription regulator CcpA by the phosphoprotein HPr-Ser46-P.
Cell 2004;118(6):731-41.
2004: Drueppel Lars; Pfleiderer Klaus; Schmidt Andreas; Hillen Wolfgang; Berens Christian
A short autonomous repression motif is located within the N-terminal domain of CTCF.
FEBS letters 2004;572(1-3):154-8.
2004: Scholz Oliver; Henssler Eva-Maria; Bail Johannes; Schubert Peter; Bogdanska-Urbaniak Joanna; Sopp Sabine; Reich Marco; Wisshak Stefanie; Köstner Martin; Bertram Ralph; Hillen Wolfgang
Activity reversal of Tet repressor caused by single amino acid exchanges.
Molecular microbiology 2004;53(3):777-89.
2004: Henssler Eva-Maria; Scholz Oliver; Lochner Susanne; Gmeiner Peter; Hillen Wolfgang
Structure-based design of Tet repressor to optimize a new inducer specificity.
Biochemistry 2004;43(29):9512-8.
2004: Kamionka Annette; Sehnal Miriam; Scholz Oliver; Hillen Wolfgang
Independent regulation of two genes in Escherichia coli by tetracyclines and Tet repressor variants.
Journal of bacteriology 2004;186(13):4399-401.
2004: Schubert Peter; Pfleiderer Klaus; Hillen Wolfgang
Tet repressor residues indirectly recognizing anhydrotetracycline.
European journal of biochemistry / FEBS 2004;271(11):2144-52.
2004: Krueger Christel; Schmidt Andreas; Danke Christina; Hillen Wolfgang; Berens Christian
Transactivator mutants with altered effector specificity allow selective regulation of two genes by tetracycline variants.
Gene 2004;331():125-31.
2004: Zabala Maider; Wang Lin; Hernandez-Alcoceba Ruben; Hillen Wolfgang; Qian Cheng; Prieto Jesus; Kramer M Gabriela
Optimization of the Tet-on system to regulate interleukin 12 expression in the liver for the treatment of hepatic tumors.
Cancer research 2004;64(8):2799-804.
2004: Bauer Gesine; Berens Christian; Projan Steven J; Hillen Wolfgang
Comparison of tetracycline and tigecycline binding to ribosomes mapped by dimethylsulphate and drug-directed Fe2+ cleavage of 16S rRNA.
The Journal of antimicrobial chemotherapy 2004;53(4):592-9.
2004: Esteban Carlos D; Mahr Kerstin; Monedero Vicente; Hillen Wolfgang; Pérez-Martínez Gaspar; Titgemeyer Fritz
Complementation of a Delta ccpA mutant of Lactobacillus casei with CcpA mutants affected in the DNA- and cofactor-binding domains.
Microbiology (Reading, England) 2004;150(Pt 3):613-20.
2004: Leypold Clemens F; Marian Dan-Thomas; Roman Ciprian; Schneider Siegfried; Schubert Peter; Scholz Oliver; Hillen Wolfgang; Clark Timothy; Lanig Harald
How does Mg(2+) affect the binding of anhydrotetracycline in the TetR protein?
Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology 2004;3(1):109-19.
2004: Merzbacher Matthias; Detsch Christian; Hillen Wolfgang; Stülke Jörg
Mycoplasma pneumoniae HPr kinase/phosphorylase.
European journal of biochemistry / FEBS 2004;271(2):367-74.
2004: Bertram Ralph; Kraft Christine; Wisshak Stefanie; Mueller Judith; Scholz Oliver; Hillen Wolfgang
Phenotypes of combined tet repressor mutants for effector and operator recognition and allostery.
Journal of molecular microbiology and biotechnology 2004;8(2):104-10.
2004: Berens Christian; Hillen Wolfgang
Gene regulation by tetracyclines.
Genetic engineering 2004;26():255-77.
2004: Suess Beatrix; Fink Barbara; Berens Christian; Stentz Régis; Hillen Wolfgang
A theophylline responsive riboswitch based on helix slipping controls gene expression in vivo.
Nucleic acids research 2004;32(4):1610-4.
2004: Kamionka Annette; Bogdanska-Urbaniak Joanna; Scholz Oliver; Hillen Wolfgang
Two mutations in the tetracycline repressor change the inducer anhydrotetracycline to a corepressor.
Nucleic acids research 2004;32(2):842-7.
2003: Yao Shiyi; Gao Xuewen; Fuchsbauer Norbert; Hillen Wolfgang; Vater Joachim; Wang Jinsheng
Cloning, sequencing, and characterization of the genetic region relevant to biosynthesis of the lipopeptides iturin A and surfactin in Bacillus subtilis.
Current microbiology 2003;47(4):272-7.
2003: Fechner H; Wang X; Srour M; Siemetzki U; Seltmann H; Sutter A P; Scherübl H; Zouboulis C C; Schwaab R; Hillen W; Schultheiss H-P; Poller W
A novel tetracycline-controlled transactivator-transrepressor system enables external control of oncolytic adenovirus replication.
Gene therapy 2003;10(19):1680-90.
2003: Berens Christian; Hillen Wolfgang
Gene regulation by tetracyclines. Constraints of resistance regulation in bacteria shape TetR for application in eukaryotes.
European journal of biochemistry / FEBS 2003;270(15):3109-21.
2003: Krueger Christel; Berens Christian; Schmidt Andreas; Schnappinger Dirk; Hillen Wolfgang
Single-chain Tet transregulators.
Nucleic acids research 2003;31(12):3050-6.
2003: Scholz Oliver; Köstner Martin; Reich Marco; Gastiger Susanne; Hillen Wolfgang
Teaching TetR to recognize a new inducer.
Journal of molecular biology 2003;329(2):217-27.
2003: Knott Anja; Müller Yvonne; Zunino Susan J; Berens Christian; Hillen Wolfgang
Conditional cell suicide using dox-dependent caspase-2 expression.
The journal of gene medicine 2003;5(4):343-54.
2003: Suess Beatrix; Hanson Shane; Berens Christian; Fink Barbara; Schroeder Renée; Hillen Wolfgang
Conditional gene expression by controlling translation with tetracycline-binding aptamers.
Nucleic acids research 2003;31(7):1853-8.
2003: Koponen J K; Kankkonen H; Kannasto J; Wirth T; Hillen W; Bujard H; Ylä-Herttuala S
Doxycycline-regulated lentiviral vector system with a novel reverse transactivator rtTA2S-M2 shows a tight control of gene expression in vitro and in vivo.
Gene therapy 2003;10(6):459-66.
2003: Allen Gregory S; Steinhauer Katrin; Hillen Wolfgang; Stülke Jörg; Brennan Richard G
Crystal structure of HPr kinase/phosphatase from Mycoplasma pneumoniae.
Journal of molecular biology 2003;326(4):1203-17.
2002: Marian Dan-Thomas; Leypold Clemens; Kunz Michael; Schneider Siegfried; Schubert Peter; Scholz Oliver; Hillen Wolfgang
Pressure effect on the structure of the Tet repressor protein TetR(B).
Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology 2002;1(11):841-51.
2002: Salucci V; Scarito A; Aurisicchio L; Lamartina S; Nicolaus G; Giampaoli S; Gonzalez-Paz O; Toniatti C; Bujard H; Hillen W; Ciliberto G; Palombo F
Tight control of gene expression by a helper-dependent adenovirus vector carrying the rtTA2(s)-M2 tetracycline transactivator and repressor system.
Gene therapy 2002;9(21):1415-21.
2002: Steinhauer Katrin; Jepp Tanja; Hillen Wolfgang; Stülke Jörg
A novel mode of control of Mycoplasma pneumoniae HPr kinase/phosphatase activity reflects its parasitic lifestyle.
Microbiology (Reading, England) 2002;148(Pt 10):3277-84.
2002: Mahr Kerstin; Esteban Carlos David; Hillen Wolfgang; Titgemeyer Fritz; Pérez-Martínez Gaspar
Cross communication between components of carbon catabolite repression of Lactobacillus casei and Bacillus megaterium.
Journal of molecular microbiology and biotechnology 2002;4(5):489-94.
2002: Titgemeyer Fritz; Hillen Wolfgang
Global control of sugar metabolism: a gram-positive solution.
Antonie van Leeuwenhoek 2002;82(1-4):59-71.
2002: Hanson K G; Steinhauer Katrin; Reizer Jonathan; Hillen Wolfgang; Stülke Jörg
HPr kinase/phosphatase of Bacillus subtilis: expression of the gene and effects of mutations on enzyme activity, growth and carbon catabolite repression.
Microbiology (Reading, England) 2002;148(Pt 6):1805-11.
2002: Aung-Hilbrich Lwin Mar; Seidel Gerald; Wagner Andrea; Hillen Wolfgang
Quantification of the influence of HPrSer46P on CcpA-cre interaction.
Journal of molecular biology 2002;319(1):77-85.
2002: Knott A; Garke K; Urlinger S; Guthmann J; Müller Y; Thellmann M; Hillen W
Tetracycline-dependent gene regulation: combinations of transregulators yield a variety of expression windows.
BioTechniques 2002;32(4):796, 798, 800 passim.
2002: Steinhauer Katrin; Allen Gregory S; Hillen Wolfgang; Stülke Jörg; Brennan Richard G
Crystallization, preliminary X-ray analysis and biophysical characterization of HPr kinase/phosphatase of Mycoplasma pneumoniae.
Acta crystallographica. Section D, Biological crystallography 2002;58(Pt 3):515-8.
2002: Lamartina Stefania; Roscilli Giuseppe; Rinaudo Cira Daniela; Sporeno Elisabetta; Silvi Luisa; Hillen Wolfgang; Bujard Hermann; Cortese Riccardo; Ciliberto Gennaro; Toniatti Carlo
Stringent control of gene expression in vivo by using novel doxycycline-dependent trans-activators.
Human gene therapy 2002;13(2):199-210.
2001: Aurisicchio L; Bujard H; Hillen W; Cortese R; Ciliberto G; La Monica N; Palombo F
Regulated and prolonged expression of mIFN(alpha) in immunocompetent mice mediated by a helper-dependent adenovirus vector.
Gene therapy 2001;8(24):1817-25.
2001: Stebbins M J; Urlinger S; Byrne G; Bello B; Hillen W; Yin J C
Tetracycline-inducible systems for Drosophila.
Proceedings of the National Academy of Sciences of the United States of America 2001;98(19):10775-80.
2001: Scholz O; Kintrup M; Reich M; Hillen W
Mechanism of Tet repressor induction by tetracyclines: length compensates for sequence in the alpha8-alpha9 loop.
Journal of molecular biology 2001;310(5):979-86.
2001: Schubert P; Schnappinger D; Pfleiderer K; Hillen W
Identification of a stability determinant on the edge of the Tet repressor four-helix bundle dimerization motif.
Biochemistry 2001;40(11):3257-63.
2000: Wagner A; Küster-Schöck E; Hillen W
Sugar uptake and carbon catabolite repression in Bacillus megaterium strains with inactivated ptsHI.
Journal of molecular microbiology and biotechnology 2000;2(4):587-92.
2000: Scholz O; Schubert P; Kintrup M; Hillen W
Tet repressor induction without Mg2+.
Biochemistry 2000;39(35):10914-20.
2000: Kunz M; Kintrup M; Hillen W; Schneider S
Conformational changes induced in the Tet repressor protein TetR(B) upon operator or anhydrotetracycline binding as revealed by time-resolved fluorescence spectroscopy on single tryptophan mutants.
Photochemistry and photobiology 2000;72(1):35-48.
2000: Urlinger S; Baron U; Thellmann M; Hasan M T; Bujard H; Hillen W
Exploring the sequence space for tetracycline-dependent transcriptional activators: novel mutations yield expanded range and sensitivity.
Proceedings of the National Academy of Sciences of the United States of America 2000;97(14):7963-8.
2000: Tiebel B; Garke K; Hillen W
Observing conformational and activity changes of tet repressor in vivo.
Nature structural biology 2000;7(6):479-81.
2000: Urlinger S; Helbl V; Guthmann J; Pook E; Grimm S; Hillen W
The p65 domain from NF-kappaB is an efficient human activator in the tetracycline-regulatable gene expression system.
Gene 2000;247(1-2):103-10.
2000: Scholz O; Thiel A; Hillen W; Niederweis M
Quantitative analysis of gene expression with an improved green fluorescent protein. p6.
European journal of biochemistry / FEBS 2000;267(6):1565-70.
2000: Vergani B; Kintrup M; Hillen W; Lami H; Piémont E; Bombarda E; Alberti P; Doglia S M; Chabbert M
Backbone dynamics of Tet repressor alpha8intersectionalpha9 loop.
Biochemistry 2000;39(10):2759-68.
2000: Orth P; Schnappinger D; Hillen W; Saenger W; Hinrichs W
Structural basis of gene regulation by the tetracycline inducible Tet repressor-operator system.
Nature structural biology 2000;7(3):215-9.
2000: Kintrup M; Schubert P; Kunz M; Chabbert M; Alberti P; Bombarda E; Schneider S; Hillen W
Trp scanning analysis of Tet repressor reveals conformational changes associated with operator and anhydrotetracycline binding.
European journal of biochemistry / FEBS 2000;267(3):821-9.
2000: Tebbe J; Orth P; Küster-Schöck E K; Hillen W; Saenger W; Hinrichs W
Crystallization and preliminary X-ray analyses of catabolite control protein A, free and in complex with its DNA-binding site.
Acta crystallographica. Section D, Biological crystallography 2000;56(Pt 1):67-9.
2000: Sigler A; Schubert P; Hillen W; Niederweis M
Permeation of tetracyclines through membranes of liposomes and Escherichia coli.
European journal of biochemistry / FEBS 2000;267(2):527-34.
2000: Mahr K; Hillen W; Titgemeyer F
Carbon catabolite repression in Lactobacillus pentosus: analysis of the ccpA region.
Applied and environmental microbiology 2000;66(1):277-83.
2000: Stülke J; Hillen W
Regulation of carbon catabolism in Bacillus species.
Annual review of microbiology 2000;54():849-80.
1999: Küster-Schöck E; Wagner A; Völker U; Hillen W
Mutations in catabolite control protein CcpA showing glucose-independent regulation in Bacillus megaterium.
Journal of bacteriology 1999;181(24):7634-8.
1999: Trelstad P L; Purdhani P; Geissdörfer W; Hillen W; Keasling J D
Polyphosphate kinase of Acinetobacter sp. strain ADP1: purification and characterization of the enzyme and its role during changes in extracellular phosphate levels.
Applied and environmental microbiology 1999;65(9):3780-6.
1999: Geissdörfer W; Kok R G; Ratajczak A; Hellingwerf K J; Hillen W
The genes rubA and rubB for alkane degradation in Acinetobacter sp. strain ADP1 are in an operon with estB, encoding an esterase, and oxyR.
Journal of bacteriology 1999;181(14):4292-8.
1999: Tiebel B; Radzwill N; Aung-Hilbrich L M; Helbl V; Steinhoff H J; Hillen W
Domain motions accompanying Tet repressor induction defined by changes of interspin distances at selectively labeled sites.
Journal of molecular biology 1999;290(1):229-40.
1999: Küster E; Hilbich T; Dahl M K; Hillen W
Mutations in catabolite control protein CcpA separating growth effects from catabolite repression.
Journal of bacteriology 1999;181(13):4125-8.
1999: Levy S B; McMurry L M; Barbosa T M; Burdett V; Courvalin P; Hillen W; Roberts M C; Rood J I; Taylor D E
Nomenclature for new tetracycline resistance determinants.
Antimicrobial agents and chemotherapy 1999;43(6):1523-4.
1999: Stülke J; Hillen W
Carbon catabolite repression in bacteria.
Current opinion in microbiology 1999;2(2):195-201.
1999: Schnappinger D; Schubert P; Berens C; Pfleiderer K; Hillen W
Solvent-exposed residues in the Tet repressor (TetR) four-helix bundle contribute to subunit recognition and dimer stability.
The Journal of biological chemistry 1999;274(10):6405-10.
1999: Baron U; Schnappinger D; Helbl V; Gossen M; Hillen W; Bujard H
Generation of conditional mutants in higher eukaryotes by switching between the expression of two genes.
Proceedings of the National Academy of Sciences of the United States of America 1999;96(3):1013-8.
1999: Orth P; Schnappinger D; Sum P E; Ellestad G A; Hillen W; Saenger W; Hinrichs W
Crystal structure of the tet repressor in complex with a novel tetracycline, 9-(N,N-dimethylglycylamido)- 6-demethyl-6-deoxy-tetracycline.
Journal of molecular biology 1999;285(2):455-61.
1999: Forster K; Helbl V; Lederer T; Urlinger S; Wittenburg N; Hillen W
Tetracycline-inducible expression systems with reduced basal activity in mammalian cells.
Nucleic acids research 1999;27(2):708-10.
1998: Pook E; Grimm S; Bonin A; Winkler T; Hillen W
Affinities of mAbs to Tet repressor complexed with operator or tetracycline suggest conformational changes associated with induction.
European journal of biochemistry / FEBS 1998;258(3):915-22.
1998: Kraus A; Küster E; Wagner A; Hoffmann K; Hillen W
Identification of a co-repressor binding site in catabolite control protein CcpA.
Molecular microbiology 1998;30(5):955-63.
1998: Stülke J; Hillen W
Coupling physiology and gene regulation in bacteria: the phosphotransferase sugar uptake system delivers the signals.
Die Naturwissenschaften 1998;85(12):583-92.
1998: Ratajczak A; Geissdörfer W; Hillen W
Expression of alkane hydroxylase from Acinetobacter sp. Strain ADP1 is induced by a broad range of n-alkanes and requires the transcriptional activator AlkR.
Journal of bacteriology 1998;180(22):5822-7.
1998: Tiebel B; Aung-Hilbrich L M; Schnappinger D; Hillen W
Conformational changes necessary for gene regulation by Tet repressor assayed by reversible disulfide bond formation.
The EMBO journal 1998;17(17):5112-9.
1998: Orth P; Cordes F; Schnappinger D; Hillen W; Saenger W; Hinrichs W
Conformational changes of the Tet repressor induced by tetracycline trapping.
Journal of molecular biology 1998;279(2):439-47.
1998: Ratajczak A; Geissdörfer W; Hillen W
Alkane hydroxylase from Acinetobacter sp. strain ADP1 is encoded by alkM and belongs to a new family of bacterial integral-membrane hydrocarbon hydroxylases.
Applied and environmental microbiology 1998;64(4):1175-9.
1998: Reizer J; Hoischen C; Titgemeyer F; Rivolta C; Rabus R; Stülke J; Karamata D; Saier M H; Hillen W
A novel protein kinase that controls carbon catabolite repression in bacteria.
Molecular microbiology 1998;27(6):1157-69.
1998: Berens C; Streicher B; Schroeder R; Hillen W
Visualizing metal-ion-binding sites in group I introns by iron(II)-mediated Fenton reactions.
Chemistry & biology 1998;5(3):163-75.
1998: Geissdörfer W; Ratajczak A; Hillen W
Transcription of ppk from Acinetobacter sp. strain ADP1, encoding a putative polyphosphate kinase, is induced by phosphate starvation.
Applied and environmental microbiology 1998;64(3):896-901.
1998: Schirmer F; Hillen W
The Acinetobacter calcoaceticus NCIB8250 mop operon mRNA is differentially degraded, resulting in a higher level of the 3' CatA-encoding segment than of the 5' phenolhydroxylase-encoding portion.
Molecular & general genetics : MGG 1998;257(3):330-7.
1998: Helbl V; Tiebel B; Hillen W
Stepwise selection of TetR variants recognizing tet operator 6C with high affinity and specificity.
Journal of molecular biology 1998;276(2):319-24.
1998: Helbl V; Hillen W
Stepwise selection of TetR variants recognizing tet operator 4C with high affinity and specificity.
Journal of molecular biology 1998;276(2):313-8.
1998: Biburger M; Berens C; Lederer T; Krec T; Hillen W
Intragenic suppressors of induction-deficient TetR mutants: localization and potential mechanism of action.
Journal of bacteriology 1998;180(3):737-41.
1998: Schnappinger D; Schubert P; Pfleiderer K; Hillen W
Determinants of protein-protein recognition by four helix bundles: changing the dimerization specificity of Tet repressor.
The EMBO journal 1998;17(2):535-43.
1997: Späth C; Kraus A; Hillen W
Contribution of glucose kinase to glucose repression of xylose utilization in Bacillus megaterium.
Journal of bacteriology 1997;179(23):7603-5.
1997: Alberti P; Bombarda E; Kintrup M; Hillen W; Lami H; Piémont E; Doglia S M; Chabbert M
Structural investigation of Tet repressor loop 154-167: a time-resolved fluorescence study of three single Trp mutants.
Archives of biochemistry and biophysics 1997;346(2):230-40.
1997: Jones B E; Dossonnet V; Küster E; Hillen W; Deutscher J; Klevit R E
Binding of the catabolite repressor protein CcpA to its DNA target is regulated by phosphorylation of its corepressor HPr.
The Journal of biological chemistry 1997;272(42):26530-5.
1997: Kraus A; Hillen W
Analysis of CcpA mutations defective in carbon catabolite repression in Bacillus megaterium.
FEMS microbiology letters 1997;153(1):221-6.
1997: Parche S; Geissdörfer W; Hillen W
Identification and characterization of xcpR encoding a subunit of the general secretory pathway necessary for dodecane degradation in Acinetobacter calcoaceticus ADP1.
Journal of bacteriology 1997;179(14):4631-4.
1997: Schmiedel D; Vary P S; Jablonski L; Hillen W
Plasmids for efficient single-copy gene cloning into gdh2 and trpC of Bacillus megaterium DSM319 and QM B1551.
Applied microbiology and biotechnology 1997;47(5):543-6.
1997: Backes H; Berens C; Helbl V; Walter S; Schmid F X; Hillen W
Combinations of the alpha-helix-turn-alpha-helix motif of TetR with respective residues from LacI or 434Cro: DNA recognition, inducer binding, and urea-dependent denaturation.
Biochemistry 1997;36(18):5311-22.
1997: Silvi Antonini P; Hillen W; Ettner N; Hinrichs W; Fantucci P; Doglia S M; Bousquet J A; Chabbert M
Molecular mechanics analysis of Tet repressor TRP-43 fluorescence.
Biophysical journal 1997;72(4):1800-11.
1997: Gösseringer R; Küster E; Galinier A; Deutscher J; Hillen W
Cooperative and non-cooperative DNA binding modes of catabolite control protein CcpA from Bacillus megaterium result from sensing two different signals.
Journal of molecular biology 1997;266(4):665-76.
1997: Schmiedel D; Kintrup M; Küster E; Hillen W
Regulation of expression, genetic organization and substrate specificity of xylose uptake in Bacillus megaterium.
Molecular microbiology 1997;23(5):1053-62.
1997: Berens C; Schnappinger D; Hillen W
The role of the variable region in Tet repressor for inducibility by tetracycline.
The Journal of biological chemistry 1997;272(11):6936-42.
1997: Geissdörfer W; Ratajczak A; Hillen W
Nucleotide sequence of a putative periplasmic Mn superoxide dismutase from Acinetobacter calcoaceticus ADP1.
Gene 1997;186(2):305-8.
1997: Schirmer F; Ehrt S; Hillen W
Expression, inducer spectrum, domain structure, and function of MopR, the regulator of phenol degradation in Acinetobacter calcoaceticus NCIB8250.
Journal of bacteriology 1997;179(4):1329-36.
1996: Reizer J; Bergstedt U; Galinier A; Küster E; Saier M H; Hillen W; Steinmetz M; Deutscher J
Catabolite repression resistance of gnt operon expression in Bacillus subtilis conferred by mutation of His-15, the site of phosphoenolpyruvate-dependent phosphorylation of the phosphocarrier protein HPr.
Journal of bacteriology 1996;178(18):5480-6.
1996: Ettner N; Müller G; Berens C; Backes H; Schnappinger D; Schreppel T; Pfleiderer K; Hillen W
Fast large-scale purification of tetracycline repressor variants from overproducing Escherichia coli strains.
Journal of chromatography. A 1996;742(1-2):95-105.
1996: Küster E; Luesink E J; de Vos W M; Hillen W
Immunological crossreactivity to the catabolite control protein CcpA Bacillus megaterium is found in many gram-positive bacteria.
FEMS microbiology letters 1996;139(2-3):109-15.
1996: Schnappinger D; Hillen W
Tetracyclines: antibiotic action, uptake, and resistance mechanisms.
Archives of microbiology 1996;165(6):359-69.
1996: Lederer T; Kintrup M; Takahashi M; Sum P E; Ellestad G A; Hillen W
Tetracycline analogs affecting binding to Tn10-Encoded Tet repressor trigger the same mechanism of induction.
Biochemistry 1996;35(23):7439-46.
1996: Schmiedel D; Hillen W
Contributions of XylR CcpA and cre to diauxic growth of Bacillus megaterium and to xylose isomerase expression in the presence of glucose and xylose.
Molecular & general genetics : MGG 1996;250(3):259-66.
1995: Lederer T; Takahashi M; Hillen W
Thermodynamic analysis of tetracycline-mediated induction of Tet repressor by a quantitative methylation protection assay.
Analytical biochemistry 1995;232(2):190-6.
1995: Berens C; Pfleiderer K; Helbl V; Hillen W
Deletion mutagenesis of Tn 10 Tet repressor--localization of regions important for dimerization and inducibility in vivo.
Molecular microbiology 1995;18(3):437-48.
1995: Ehrt S; Schirmer F; Hillen W
Genetic organization, nucleotide sequence and regulation of expression of genes encoding phenol hydroxylase and catechol 1,2-dioxygenase in Acinetobacter calcoaceticus NCIB8250.
Molecular microbiology 1995;18(1):13-20.
1995: Dahl M K; Schmiedel D; Hillen W
Glucose and glucose-6-phosphate interaction with Xyl repressor proteins from Bacillus spp. may contribute to regulation of xylose utilization.
Journal of bacteriology 1995;177(19):5467-72.
1995: Peviani C; Hillen W; Ettner N; Lami H; Doglia S M; Piémont E; Ellouze C; Chabbert M
Spectroscopic investigation of Tet repressor tryptophan-43 upon specific and nonspecific DNA binding.
Biochemistry 1995;34(40):13007-15.
1995: Müller G; Hecht B; Helbl V; Hinrichs W; Saenger W; Hillen W
Characterization of non-inducible Tet repressor mutants suggests conformational changes necessary for induction.
Nature structural biology 1995;2(8):693-703.
1995: Gossen M; Freundlieb S; Bender G; Müller G; Hillen W; Bujard H
Transcriptional activation by tetracyclines in mammalian cells.
Science (New York, N.Y.) 1995;268(5218):1766-9.
1995: Geissdörfer W; Frosch S C; Haspel G; Ehrt S; Hillen W
Two genes encoding proteins with similarities to rubredoxin and rubredoxin reductase are required for conversion of dodecane to lauric acid in Acinetobacter calcoaceticus ADP1.
Microbiology (Reading, England) 1995;141 ( Pt 6)():1425-32.
1995: Ramseier T M; Reizer J; Küster E; Hillen W; Saier M H
In vitro binding of the CcpA protein of Bacillus megaterium to cis-acting catabolite responsive elements (CREs) of gram-positive bacteria.
FEMS microbiology letters 1995;129(2-3):207-13.
1995: Schnappinger D; Geissdörfer W; Sizemore C; Hillen W
Extracellular expression of native human anti-lysozyme fragments in Staphylococcus carnosus.
FEMS microbiology letters 1995;129(2-3):121-7.
1995: Hueck C J; Kraus A; Schmiedel D; Hillen W
Cloning, expression and functional analyses of the catabolite control protein CcpA from Bacillus megaterium.
Molecular microbiology 1995;16(5):855-64.
1995: Kim H J; Gatz C; Hillen W; Jones T R
Tetracycline repressor-regulated gene repression in recombinant human cytomegalovirus.
Journal of virology 1995;69(4):2565-73.
1995: Kisker C; Hinrichs W; Tovar K; Hillen W; Saenger W
The complex formed between Tet repressor and tetracycline-Mg2+ reveals mechanism of antibiotic resistance.
Journal of molecular biology 1995;247(2):260-80.
1995: Deutscher J; Küster E; Bergstedt U; Charrier V; Hillen W
Protein kinase-dependent HPr/CcpA interaction links glycolytic activity to carbon catabolite repression in gram-positive bacteria.
Molecular microbiology 1995;15(6):1049-53.
1995: Helbl V; Berens C; Hillen W
Proximity probing of Tet repressor to tet operator by dimethylsulfate reveals protected and accessible functions for each recognized base-pair in the major groove.
Journal of molecular biology 1995;245(5):538-48.
1995: Hueck C J; Hillen W
Catabolite repression in Bacillus subtilis: a global regulatory mechanism for the gram-positive bacteria?
Molecular microbiology 1995;15(3):395-401.
1995: Ettner N; Metzger J W; Lederer T; Hulmes J D; Kisker C; Hinrichs W; Ellestad G A; Hillen W
Proximity mapping of the Tet repressor-tetracycline-Fe2+ complex by hydrogen peroxide mediated protein cleavage.
Biochemistry 1995;34(1):22-31.
1994: Dahl M K; Degenkolb J; Hillen W
Transcription of the xyl operon is controlled in Bacillus subtilis by tandem overlapping operators spaced by four base-pairs.
Journal of molecular biology 1994;243(3):413-24.
1994: Hueck C J; Hillen W; Saier M H
Analysis of a cis-active sequence mediating catabolite repression in gram-positive bacteria.
Research in microbiology 1994;145(7):503-18.
1994: Scheler A; Hillen W
Regulation of xylose utilization in Bacillus licheniformis: Xyl repressor-xyl-operator interaction studied by DNA modification protection and interference.
Molecular microbiology 1994;13(3):505-12.
1994: Ehrt S; Ornston L N; Hillen W
RpoN (sigma 54) is required for conversion of phenol to catechol in Acinetobacter calcoaceticus.
Journal of bacteriology 1994;176(12):3493-9.
1994: Biburger M; Niederweis M; Hillen W
Oligo[d(C).(G)] runs exhibit a helical repeat of 11.1 bp in solution and cause slight DNA curvature when properly phased.
Nucleic acids research 1994;22(9):1562-6.
1994: Hueck C; Kraus A; Hillen W
Sequences of ccpA and two downstream Bacillus megaterium genes with homology to the motAB operon from Bacillus subtilis.
Gene 1994;143(1):147-8.
1994: Ehrt S; Hillen W
UDP-N-acetylglucosamine 1-carboxyvinyl-transferase from Acinetobacter calcoaceticus.
FEMS microbiology letters 1994;117(2):137-42.
1994: Hinrichs W; Kisker C; Düvel M; Müller A; Tovar K; Hillen W; Saenger W
Structure of the Tet repressor-tetracycline complex and regulation of antibiotic resistance.
Science (New York, N.Y.) 1994;264(5157):418-20.
1994: Niederweis M; Lederer T; Hillen W
Matrix effects suggest an important influence of DNA-polyacrylamide interactions on the electrophoretic mobility of DNA.
The Journal of biological chemistry 1994;269(13):10156-62.
1994: Weinmann P; Gossen M; Hillen W; Bujard H; Gatz C
A chimeric transactivator allows tetracycline-responsive gene expression in whole plants.
The Plant journal : for cell and molecular biology 1994;5(4):559-69.
1994: Kraus A; Hueck C; Gärtner D; Hillen W
Catabolite repression of the Bacillus subtilis xyl operon involves a cis element functional in the context of an unrelated sequence, and glucose exerts additional xylR-dependent repression.
Journal of bacteriology 1994;176(6):1738-45.
1994: Hillen W; Berens C
Mechanisms underlying expression of Tn10 encoded tetracycline resistance.
Annual review of microbiology 1994;48():345-69.
1993: Niederweis M; Hillen W
Electrophoretic analysis of protein-induced DNA bending and twist changes.
Electrophoresis 1993;14(8):693-8.
1993: Kauder C; Allmansberger R; Gärtner D; Schmiedel D; Hillen W
An operator binding-negative mutation of Xyl repressor from Bacillus subtilis is trans dominant in Bacillus megaterium.
FEMS microbiology letters 1993;109(1):81-4.
1993: Sizemore C; Geissdörfer W; Hillen W
Using fusions with luxAB from Vibrio harveyi MAV to quantify induction and catabolite repression of the xyl operon in Staphylococcus carnosus TM300.
FEMS microbiology letters 1993;107(2-3):303-6.
1993: Hecht B; Müller G; Hillen W
Noninducible Tet repressor mutations map from the operator binding motif to the C terminus.
Journal of bacteriology 1993;175(4):1206-10.
1992: Niederweis M; Lederer T; Hillen W
An accurate method for determining the helical repeat of DNA in solution reveals differences to the crystal structures of two B-DNA decamers.
Journal of molecular biology 1992;228(2):322-6.
1992: Baumeister R; Müller G; Hecht B; Hillen W
Functional roles of amino acid residues involved in forming the alpha-helix-turn-alpha-helix operator DNA binding motif of Tet repressor from Tn10.
Proteins 1992;14(2):168-77.
1992: Baumeister R; Helbl V; Hillen W
Contacts between Tet repressor and tet operator revealed by new recognition specificities of single amino acid replacement mutants.
Journal of molecular biology 1992;226(4):1257-70.
1992: Rygus T; Hillen W
Catabolite repression of the xyl operon in Bacillus megaterium.
Journal of bacteriology 1992;174(9):3049-55.
1992: Sizemore C; Wieland B; Götz F; Hillen W
Regulation of Staphylococcus xylosus xylose utilization genes at the molecular level.
Journal of bacteriology 1992;174(9):3042-8.
1992: Gülland U; Hillen W
The Tn10-encoded tetR mRNA has heterogeneous 5' ends in vivo and in vitro.
Gene 1992;114(1):97-101.
1992: Gärtner D; Degenkolb J; Ripperger J A; Allmansberger R; Hillen W
Regulation of the Bacillus subtilis W23 xylose utilization operon: interaction of the Xyl repressor with the xyl operator and the inducer xylose.
Molecular & general genetics : MGG 1992;232(3):415-22.
1992: Dingermann T; Frank-Stoll U; Werner H; Wissmann A; Hillen W; Jacquet M; Marschalek R
RNA polymerase III catalysed transcription can be regulated in Saccharomyces cerevisiae by the bacterial tetracycline repressor-operator system.
The EMBO journal 1992;11(4):1487-92.
1992: Flache P; Baumeister R; Hillen W
The Tn10-encoded tetracycline resistance mRNA contains a translational silencer in the 5' nontranslated region.
Journal of bacteriology 1992;174(8):2478-84.
1992: Chabbert M; Hillen W; Hansen D; Takahashi M; Bousquet J A
Structural analysis of the operator binding domain of Tn10-encoded Tet repressor: a time-resolved fluorescence and anisotropy study.
Biochemistry 1992;31(7):1951-60.
1992: Berens C; Altschmied L; Hillen W
The role of the N terminus in Tet repressor for tet operator binding determined by a mutational analysis.
The Journal of biological chemistry 1992;267(3):1945-52.
1991: Takahashi M; Degenkolb J; Hillen W
Determination of the equilibrium association constant between Tet repressor and tetracycline at limiting Mg2+ concentrations: a generally applicable method for effector-dependent high-affinity complexes.
Analytical biochemistry 1991;199(2):197-202.
1991: Wissmann A; Baumeister R; Müller G; Hecht B; Helbl V; Pfleiderer K; Hillen W
Amino acids determining operator binding specificity in the helix-turn-helix motif of Tn10 Tet repressor.
The EMBO journal 1991;10(13):4145-52.
1991: Jacob S; Allmansberger R; Gärtner D; Hillen W
Catabolite repression of the operon for xylose utilization from Bacillus subtilis W23 is mediated at the level of transcription and depends on a cis site in the xylA reading frame.
Molecular & general genetics : MGG 1991;229(2):189-96.
1991: Baumeister R; Flache P; Melefors O; von Gabain A; Hillen W
Lack of a 5' non-coding region in Tn1721 encoded tetR mRNA is associated with a low efficiency of translation and a short half-life in Escherichia coli.
Nucleic acids research 1991;19(17):4595-600.
1991: Degenkolb J; Takahashi M; Ellestad G A; Hillen W
Structural requirements of tetracycline-Tet repressor interaction: determination of equilibrium binding constants for tetracycline analogs with the Tet repressor.
Antimicrobial agents and chemotherapy 1991;35(8):1591-5.
1991: Rygus T; Hillen W
Inducible high-level expression of heterologous genes in Bacillus megaterium using the regulatory elements of the xylose-utilization operon.
Applied microbiology and biotechnology 1991;35(5):594-9.
1991: Sizemore C; Buchner E; Rygus T; Witke C; Götz F; Hillen W
Organization, promoter analysis and transcriptional regulation of the Staphylococcus xylosus xylose utilization operon.
Molecular & general genetics : MGG 1991;227(3):377-84.
1991: Wissmann A; Wray L V; Somaggio U; Baumeister R; Geissendörfer M; Hillen W
Selection for Tn10 tet repressor binding to tet operator in Escherichia coli: isolation of temperature-sensitive mutants and combinatorial mutagenesis in the DNA binding motif.
Genetics 1991;128(2):225-32.
1991: Kleinschmidt C; Tovar K; Hillen W
Computer simulations and experimental studies of gel mobility patterns for weak and strong non-cooperative protein binding to two targets on the same DNA: application to binding of tet repressor variants to multiple and single tet operator sites.
Nucleic acids research 1991;19(5):1021-8.
1991: Surek B; Wilhelm M; Hillen W
Optimizing the promoter and ribosome binding sequence for expression of human single chain urokinase-like plasminogen activator in Escherichia coli and stabilization of the product by avoiding heat shock response.
Applied microbiology and biotechnology 1991;34(4):488-94.
1991: Scheler A; Rygus T; Allmansberger R; Hillen W
Molecular cloning, structure, promoters and regulatory elements for transcription of the Bacillus licheniformis encoded regulon for xylose utilization.
Archives of microbiology 1991;155(6):526-34.
1991: Tovar K; Hillen W
Large-scale preparation of DNA fragments for physical studies of protein binding.
Methods in enzymology 1991;208():54-63.
1991: Wissmann A; Hillen W
DNA contacts probed by modification protection and interference studies.
Methods in enzymology 1991;208():365-79.
1991: Rygus T; Scheler A; Allmansberger R; Hillen W
Molecular cloning, structure, promoters and regulatory elements for transcription of the Bacillus megaterium encoded regulon for xylose utilization.
Archives of microbiology 1991;155(6):535-42.
1990: Kishan V; Hillen W
Molecular cloning, nucleotide sequence, and promoter structure of the Acinetobacter calcoaceticus trpFB operon.
Journal of bacteriology 1990;172(10):6151-5.
1990: Geissendörfer M; Hillen W
Regulated expression of heterologous genes in Bacillus subtilis using the Tn10 encoded tet regulatory elements.
Applied microbiology and biotechnology 1990;33(6):657-63.
1990: Sizemore C; Wissmann A; Gülland U; Hillen W
Quantitative analysis of Tn10 Tet repressor binding to a complete set of tet operator mutants.
Nucleic acids research 1990;18(10):2875-80.
1990: Hunger M; Schmucker R; Kishan V; Hillen W
Analysis and nucleotide sequence of an origin of DNA replication in Acinetobacter calcoaceticus and its use for Escherichia coli shuttle plasmids.
Gene 1990;87(1):45-51.
1990: Haspel G; Hunger M; Schmucker R; Hillen W
Identification and nucleotide sequence of the Acinetobacter calcoaceticus encoded trpE gene.
Molecular & general genetics : MGG 1990;220(3):475-7.
1989: Levy S B; McMurry L M; Burdett V; Courvalin P; Hillen W; Roberts M C; Taylor D E
Nomenclature for tetracycline resistance determinants.
Antimicrobial agents and chemotherapy 1989;33(8):1373-4.
1989: Tovar K; Hillen W
Tet repressor binding induced curvature of tet operator DNA.
Nucleic acids research 1989;17(16):6515-22.
1989: Kreuzer P; Gärtner D; Allmansberger R; Hillen W
Identification and sequence analysis of the Bacillus subtilis W23 xylR gene and xyl operator.
Journal of bacteriology 1989;171(7):3840-5.
1989: Takahashi M; Blazy B; Baudras A; Hillen W
Ligand-modulated binding of a gene regulatory protein to DNA. Quantitative analysis of cyclic-AMP induced binding of CRP from Escherichia coli to non-specific and specific DNA targets.
Journal of molecular biology 1989;207(4):783-96.
1989: Riesner D; Steger G; Zimmat R; Owens R A; Wagenhöfer M; Hillen W; Vollbach S; Henco K
Temperature-gradient gel electrophoresis of nucleic acids: analysis of conformational transitions, sequence variations, and protein-nucleic acid interactions.
Electrophoresis 1989;10(5-6):377-89.
1989: Lederer H; Tovar K; Baer G; May R P; Hillen W; Heumann H
The quaternary structure of Tet repressors bound to the Tn10-encoded tet gene control region determined by neutron solution scattering.
The EMBO journal 1989;8(4):1257-63.
1988: Tovar K; Ernst A; Hillen W
Identification and nucleotide sequence of the class E tet regulatory elements and operator and inducer binding of the encoded purified Tet repressor.
Molecular & general genetics : MGG 1988;215(1):76-80.
1988: Wagenhöfer M; Hansen D; Hillen W
Thermal denaturation of engineered tet repressor proteins and their complexes with tet operator and tetracycline studied by temperature gradient gel electrophoresis.
Analytical biochemistry 1988;175(2):422-32.
1988: Altschmied L; Baumeister R; Pfleiderer K; Hillen W
A threonine to alanine exchange at position 40 of Tet repressor alters the recognition of the sixth base pair of tet operator from GC to AT.
The EMBO journal 1988;7(12):4011-7.
1988: Heuer C; Hillen W
Tet repressor-tet operator contacts probed by operator DNA-modification interference studies.
Journal of molecular biology 1988;202(3):407-15.
1988: Wissmann A; Meier I; Hillen W
Saturation mutagenesis of the Tn10-encoded tet operator O1. Identification of base-pairs involved in Tet repressor recognition.
Journal of molecular biology 1988;202(3):397-406.
1988: Gärtner D; Geissendörfer M; Hillen W
Expression of the Bacillus subtilis xyl operon is repressed at the level of transcription and is induced by xylose.
Journal of bacteriology 1988;170(7):3102-9.
1988: Meier I; Wray L V; Hillen W
Differential regulation of the Tn10-encoded tetracycline resistance genes tetA and tetR by the tandem tet operators O1 and O2.
The EMBO journal 1988;7(2):567-72.
1988: Kleinschmidt C; Tovar K; Hillen W; Porschke D
Dynamics of repressor-operator recognition: the Tn10-encoded tetracycline resistance control.
Biochemistry 1988;27(4):1094-104.
1987: Hansen D; Altschmied L; Hillen W
Engineered Tet repressor mutants with single tryptophan residues as fluorescent probes. Solvent accessibilities of DNA and inducer binding sites and interaction with tetracycline.
The Journal of biological chemistry 1987;262(29):14030-5.
1987: Hillen W; Schiebel U; Zaengel T
Imaging performance of a digital storage phosphor system.
Medical physics 1987;14(5):744-51.
1987: Hansen D; Hillen W
Tryptophan in alpha-helix 3 of Tet repressor forms a sequence-specific contact with tet operator in solution.
The Journal of biological chemistry 1987;262(25):12269-74.
1987: Heuer C; Hickman R K; Curiale M S; Hillen W; Levy S B
Constitutive expression of tetracycline resistance mediated by a Tn10-like element in Haemophilus parainfluenzae results from a mutation in the repressor gene.
Journal of bacteriology 1987;169(3):990-4.
1986: Gatz C; Altschmied J; Hillen W
Cloning and expression of the Acinetobacter calcoaceticus mutarotase gene in Escherichia coli.
Journal of bacteriology 1986;168(1):31-9.
1986: Klock G; Hillen W
Expression, purification and operator binding of the transposon Tn1721-encoded Tet repressor.
Journal of molecular biology 1986;189(4):633-41.
1986: Wissmann A; Meier I; Wray L V; Geissendörfer M; Hillen W
Tn10 tet operator mutations affecting Tet repressor recognition.
Nucleic acids research 1986;14(10):4253-66.
1986: Gatz C; Hillen W
Acinetobacter calcoaceticus encoded mutarotase: nucleotide sequence analysis of the gene and characterization of its secretion in Escherichia coli.
Nucleic acids research 1986;14(10):4309-23.
1986: Takahashi M; Altschmied L; Hillen W
Kinetic and equilibrium characterization of the Tet repressor-tetracycline complex by fluorescence measurements. Evidence for divalent metal ion requirement and energy transfer.
Journal of molecular biology 1986;187(3):341-8.
1985: Schollmeier K; Gärtner D; Hillen W
A bidirectionally active signal for termination of transcription is located between tetA and orfL on transposon Tn10.
Nucleic acids research 1985;13(12):4227-37.
1985: Klock G; Unger B; Gatz C; Hillen W; Altenbuchner J; Schmid K; Schmitt R
Heterologous repressor-operator recognition among four classes of tetracycline resistance determinants.
Journal of bacteriology 1985;161(1):326-32.
1984: Parge H E; Schneider M; Hahn V; Saenger W; Altschmied L; Hillen W
Crystallization of and preliminary X-ray diffraction data for TET-repressor and the TET-repressor-tetracycline complex.
Journal of molecular biology 1984;180(4):1189-91.
1984: Porschke D; Hillen W; Takahashi M
The change of DNA structure by specific binding of the cAMP receptor protein from rotation diffusion and dichroism measurements.
The EMBO journal 1984;3(12):2873-8.
1984: Unger B; Becker J; Hillen W
Nucleotide sequence of the gene, protein purification and characterization of the pSC101-encoded tetracycline resistance-gene-repressor.
Gene 1984;31(1-3):103-8.
1984: Schollmeier K; Hillen W
Transposon Tn10 contains two structural genes with opposite polarity between tetA and IS10R.
Journal of bacteriology 1984;160(2):499-503.
1984: Unger B; Klock G; Hillen W
Nucleotide sequence of the repressor gene of the RA1 tetracycline resistance determinant: structural and functional comparison with three related Tet repressor genes.
Nucleic acids research 1984;12(20):7693-703.
1984: Brosius B; Riesner D; Hillen W
Interaction of nucleic acids with lipid membranes.
Journal of biomolecular structure & dynamics 1984;1(6):1535-41.
1984: Oehmichen R; Klock G; Altschmied L; Hillen W
Construction of an E. coli strain overproducing the Tn10-encoded TET repressor and its use for large scale purification.
The EMBO journal 1984;3(3):539-43.
1984: Altschmied L; Hillen W
TET repressor.tet operator complex formation induces conformational changes in the tet operator DNA.
Nucleic acids research 1984;12(4):2171-80.
1984: Hillen W; Schollmeier K; Gatz C
Control of expression of the Tn10-encoded tetracycline resistance operon. II. Interaction of RNA polymerase and TET repressor with the tet operon regulatory region.
Journal of molecular biology 1984;172(2):185-201.
1983: Hillen W; Gatz C; Altschmied L; Schollmeier K; Meier I
Control of expression of the Tn10-encoded tetracycline resistance genes. Equilibrium and kinetic investigation of the regulatory reactions.
Journal of molecular biology 1983;169(3):707-21.
1983: Takahashi M; Blazy B; Baudras A; Hillen W
On the origin of selectivity in recognition by cyclic adenosine 3',5'-monophosphate receptor protein of its specific binding site of the lactose promoter region.
Journal of molecular biology 1983;167(4):895-9.
1983: Hillen W; Schollmeier K
Nucleotide sequence of the Tn10 encoded tetracycline resistance gene.
Nucleic acids research 1983;11(2):525-39.
1983: Unger B; Clore G M; Gronenborn A M; Hillen W
Specific DNA binding of the cAMP receptor protein within the lac operon stabilizes double-stranded DNA in the presence of cAMP.
The EMBO journal 1983;2(2):289-93.
1983: Wells R D; Brennan R; Chapman K A; Goodman T C; Hart P A; Hillen W; Kellogg D R; Kilpatrick M W; Klein R D; Klysik J; Lambert P F; Larson J E; Miglietta J J; Neuendorf S K; O'Connor T R; Singleton C K; Stirdivant S M; Veneziale C M; Wartell R M; Zacharias W
Left-handed DNA helices, supercoiling, and the B-Z junction.
Cold Spring Harbor symposia on quantitative biology 1983;47 Pt 1():77-84.
1982: Hillen W; Unger B; Klock G
Analysis of tet operator-TET repressor complexes by thermal denaturation studies.
Nucleic acids research 1982;10(19):6085-97.
1982: Diekmann S; Hillen W; Morgeneyer B; Wells R D; Pörschke D
Orientation relaxation of DNA restriction fragments and the internal mobility of the double helix.
Biophysical chemistry 1982;15(4):263-70.
1982: Pörschke D; Geisler N; Hillen W
Structure of the complex between lac repressor headpiece and operator DNA from measurements of the orientation relaxation and the electric dichroism.
Nucleic acids research 1982;10(12):3791-802.
1982: Hillen W; Unger B
Binding of four repressors to double-stranded tet operator region stabilizes it against thermal denaturation.
Nature 1982;297(5868):700-2.
1982: Hillen W; Klock G; Kaffenberger I; Wray L V; Reznikoff W S
Purification of the TET repressor and TET operator from the transposon Tn10 and characterization of their interaction.
The Journal of biological chemistry 1982;257(11):6605-13.
1982: Diekmann S; Hillen W; Jung M; Wells R D; Pörschke D
Electric properties and structure of DNA-restriction fragments from measurements of the electric dichroism.
Biophysical chemistry 1982;15(2):157-67.
1982: Wartell R M; Klysik J; Hillen W; Wells R D
Junction between Z and B conformations in a DNA restriction fragment: evaluation by Raman spectroscopy.
Proceedings of the National Academy of Sciences of the United States of America 1982;79(8):2549-53.
1982: Hillen W; Unger B
Correlation of thermodynamic and genetic properties in the Tn10 encoded TET gene control region.
Nucleic acids research 1982;10(8):2685-700.
1981: Goodman T C; Hardies S C; Cortez C; Hillen W
Computer programs to assist in high resolution thermal denaturation and circular dichroism studies on nucleic acids.
Nucleic acids research 1981;9(24):7033-40.
1981: Hillen W; Goodman T C; Wells R D
Circular dichroism spectra of twelve short DNA restriction fragments of known sequence: a comparison of measured and calculated spectra.
Nucleic acids research 1981;9(13):3029-45.
1981: Early T A; Kearns D R; Hillen W; Wells R D
A 300-MHz proton nuclear magnetic resonance investigation of deoxyribonucleic acid restriction fragments: dynamic properties.
Biochemistry 1981;20(13):3764-9.
1981: Early T A; Kearns D R; Hillen W; Wells R D
A 300- and 600-MHz proton nuclear magnetic resonance investigation of a 12 base pair deoxyribonucleic acid restriction fragment: relaxation behavior of the low-field resonances in water.
Biochemistry 1981;20(13):3756-64.
1981: Hillen W; Klein R D; Wells R D
Preparation of milligram amounts of 21 deoxyribonucleic acid restriction fragments.
Biochemistry 1981;20(13):3748-56.
1981: Hillen W; Goodman T C; Benight A S; Wartell R M; Wells R D
High resolution experimental and theoretical thermal denaturation studies on small overlapping restriction fragments containing the Escherichia coli lactose genetic control region.
The Journal of biological chemistry 1981;256(6):2761-6.
1981: Hillen W; Goodman T C; Wells R D
Salt dependence and thermodynamic interpretation of the thermal denaturation of small DNA restriction fragments.
Nucleic acids research 1981;9(2):415-36.
1980: Early T A; Kearns D R; Hillen W; Wells R D
A 300 MHz and 600 MHz proton NMR study of a 12 base pair restriction fragment: investigation of structure by relaxation measurements.
Nucleic acids research 1980;8(23):5795-812.
1980: Hillen W; Wells R D
Circular dichroism studies of the B goes to A conformational transition in seven small DNA restriction fragments containing the Escherichia coli lactose control region.
Nucleic acids research 1980;8(22):5427-44.
1980: Wells R D; Goodman T C; Hillen W; Horn G T; Klein R D; Larson J E; Müller U R; Neuendorf S K; Panayotatos N; Stirdivant S M
DNA structure and gene regulation.
Progress in nucleic acid research and molecular biology 1980;24():167-267.
1979: Hardies S C; Hillen W; Goodman T C; Wells R D
High resolution thermal denaturation analyses of small sequenced DNA restriction fragments containing Escherichia coli lactose genetic control loci.
The Journal of biological chemistry 1979;254(20):10128-34.
1979: Hillen W; Gassen H G
Physical and coding properties of poly(5-methoxyuridylic) acid.
Biochimica et biophysica acta 1979;562(2):207-13.
1978: Hillen W; Egert E; Lindner H J; Gassen H G
Crystal and molecular structure of 2-thio-5 carboxymethyluridine and its methyl ester: helix terminator nucleosides in the first position of some anticodons.
Biochemistry 1978;17(24):5314-20.
1978: Hillen W; Egert E; Lindner H J; Gassen H G
Restriction or amplification of wobble recognition: the structure of 2-thio-5-methylaminomethyluridine and the interaction of odd uridines with the anticodon loop backbone.
FEBS letters 1978;94(2):361-4.
1978: Hillen W; Gassen G
5-Substituents in the uridine moiety and their effect on the conformation of ApU-type dinucleoside phosphates.
Biochimica et biophysica acta 1978;518(1):7-16.
1977: Egert E; Lindner H J; Hillen W; Gassen H G
5-Nitrouridine-monohydrate: crystal structure and conformation.
Nucleic acids research 1977;4(4):929-37.
1975: Hillen W; Gassen H G
Physical and coding properties of poly(5-aminouridylic acid) and of 5-aminouridine-containing trinucleotides.
Biochimica et biophysica acta 1975;407(3):347-56.

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