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Wim Hol

This is a preview profile on BiomedExperts - the first literature-based scientific social network. It brings the right researchers together and allows them to collaborate online. Collexis and Dell provide the BiomedExperts network of +1.5 Million pre-calculated profiles free of charge to researchers worldwide.

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Co-Publications
Name
34
Verlinde, Christophe
33
Kalk, Kor
33
Merritt, Ethan
19
Fan, Erkang
19
Holmes, Randall
15
Buckner, Frederick
15
Van Voorhis, Wesley
14
Turley, Stewart
12
Michels, Paul
12
Luft, Joseph
11
Korotkov, Konstantin
11
Roach, Claudia
11
Sarfaty, S
11
Champoux, James
10
Gelb, Michael

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Publications

TOP 3
Abendroth Jan; Kreger Allison C; Hol Wim G J
The dimer formed by the periplasmic domain of EpsL from the Type 2 Secretion System of Vibrio parahaemolyticus.
Van Voorhis Wesley C; Hol Wim G J; Myler Peter J; Stewart Lance J
The role of medical structural genomics in discovering new drugs for infectious diseases.
Korotkov Konstantin V; Gray Miranda D; Kreger Allison; Turley Stewart; Sandkvist Maria; Hol Wim G J
Calcium is essential for the major pseudopilin in the type 2 secretion system.

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All Publications

2009: Abendroth Jan; Kreger Allison C; Hol Wim G J
The dimer formed by the periplasmic domain of EpsL from the Type 2 Secretion System of Vibrio parahaemolyticus.
Journal of structural biology 2009;168(2):313-22.
2009: Van Voorhis Wesley C; Hol Wim G J; Myler Peter J; Stewart Lance J
The role of medical structural genomics in discovering new drugs for infectious diseases.
PLoS computational biology 2009;5(10):e1000530.
2009: Korotkov Konstantin V; Gray Miranda D; Kreger Allison; Turley Stewart; Sandkvist Maria; Hol Wim G J
Calcium is essential for the major pseudopilin in the type 2 secretion system.
The Journal of biological chemistry 2009;284(38):25466-70.
2009: Crowther Gregory J; Napuli Alberto J; Thomas Andrew P; Chung Diana J; Kovzun Kuzma V; Leibly David J; Castaneda Lisa J; Bhandari Janhavi; Damman Christopher J; Hui Raymond; Hol Wim G J; Buckner Frederick S; Verlinde Christophe L M J; Zhang Zhongsheng; Fan Erkang; van Voorhis Wesley C
Buffer optimization of thermal melt assays of Plasmodium proteins for detection of small-molecule ligands.
Journal of biomolecular screening : the official journal of the Society for Biomolecular Screening 2009;14(6):700-7.
2009: Abendroth Jan; Mitchell Daniel D; Korotkov Konstantin V; Johnson Tanya L; Kreger Allison; Sandkvist Maria; Hol Wim G J
The three-dimensional structure of the cytoplasmic domains of EpsF from the type 2 secretion system of Vibrio cholerae.
Journal of structural biology 2009;166(3):303-15.
2009: Lam Anita Y; Pardon Els; Korotkov Konstantin V; Hol Wim G J; Steyaert Jan
Nanobody-aided structure determination of the EpsI:EpsJ pseudopilin heterodimer from Vibrio vulnificus.
Journal of structural biology 2009;166(1):8-15.
2009: Korotkov Konstantin V; Pardon Els; Steyaert Jan; Hol Wim G J
Crystal structure of the N-terminal domain of the secretin GspD from ETEC determined with the assistance of a nanobody.
Structure (London, England : 1993) 2009;17(2):255-65.
2008: Larson Eric T; Deng Wei; Krumm Brian E; Napuli Alberto; Mueller Natascha; Van Voorhis Wesley C; Buckner Frederick S; Fan Erkang; Lauricella Angela; DeTitta George; Luft Joseph; Zucker Frank; Hol Wim G J; Verlinde Christophe L M J; Merritt Ethan A
Structures of substrate- and inhibitor-bound adenosine deaminase from a human malaria parasite show a dramatic conformational change and shed light on drug selectivity.
Journal of molecular biology 2008;381(4):975-88.
2008: Sampathkumar Parthasarathy; Roach Claudia; Michels Paul A M; Hol Wim G J
Structural insights into the recognition of peroxisomal targeting signal 1 by Trypanosoma brucei peroxin 5.
Journal of molecular biology 2008;381(4):867-80.
2008: Merritt Ethan A; Holmes Margaret; Buckner Frederick S; Van Voorhis Wesley C; Quartly Erin; Phizicky Eric M; Lauricella Angela; Luft Joseph; DeTitta George; Neely Helen; Zucker Frank; Hol Wim G J
Structure of a Trypanosoma brucei alpha/beta-hydrolase fold protein with unknown function.
Acta crystallographica. Section F, Structural biology and crystallization communications 2008;64(Pt 6):474-8.
2008: Korotkov Konstantin V; Hol Wim G J
Structure of the GspK-GspI-GspJ complex from the enterotoxigenic Escherichia coli type 2 secretion system.
Nature structural & molecular biology 2008;15(5):462-8.
2008: Wisedchaisri Goragot; Wu Meiting; Sherman David R; Hol Wim G J
Crystal structures of the response regulator DosR from Mycobacterium tuberculosis suggest a helix rearrangement mechanism for phosphorylation activation.
Journal of molecular biology 2008;378(1):227-42.
2008: Arakaki Tracy L; Buckner Frederick S; Gillespie J Robert; Malmquist Nicholas A; Phillips Margaret A; Kalyuzhniy Oleksandr; Luft Joseph R; Detitta George T; Verlinde Christophe L M J; Van Voorhis Wesley C; Hol Wim G J; Merritt Ethan A
Characterization of Trypanosoma brucei dihydroorotate dehydrogenase as a possible drug target; structural, kinetic and RNAi studies.
Molecular microbiology 2008;68(1):37-50.
2008: Yanez Marissa E; Korotkov Konstantin V; Abendroth Jan; Hol Wim G J
Structure of the minor pseudopilin EpsH from the Type 2 secretion system of Vibrio cholerae.
Journal of molecular biology 2008;377(1):91-103.
2008: Yanez Marissa E; Korotkov Konstantin V; Abendroth Jan; Hol Wim G J
The crystal structure of a binary complex of two pseudopilins: EpsI and EpsJ from the type 2 secretion system of Vibrio vulnificus.
Journal of molecular biology 2008;375(2):471-86.
2007: Bosch Jürgen; Turley Stewart; Roach Claudia M; Daly Thomas M; Bergman Lawrence W; Hol Wim G J
The closed MTIP-myosin A-tail complex from the malaria parasite invasion machinery.
Journal of molecular biology 2007;372(1):77-88.
2007: Bosch Jürgen; Buscaglia Carlos A; Krumm Brian; Ingason Bjarni P; Lucas Robert; Roach Claudia; Cardozo Timothy; Nussenzweig Victor; Hol Wim G J
Aldolase provides an unusual binding site for thrombospondin-related anonymous protein in the invasion machinery of the malaria parasite.
Proceedings of the National Academy of Sciences of the United States of America 2007;104(17):7015-20.
2007: Arakaki Tracy; Neely Helen; Boni Erica; Mueller Natasha; Buckner Frederick S; Van Voorhis Wesley C; Lauricella Angela; DeTitta George; Luft Joseph; Hol Wim G J; Merritt Ethan A
The structure of Plasmodium vivax phosphatidylethanolamine-binding protein suggests a functional motif containing a left-handed helix.
Acta crystallographica. Section F, Structural biology and crystallization communications 2007;63(Pt 3):178-82.
2007: Buscaglia Carlos A; Hol Wim G J; Nussenzweig Victor; Cardozo Timothy
Modeling the interaction between aldolase and the thrombospondin-related anonymous protein, a key connection of the malaria parasite invasion machinery.
Proteins 2007;66(3):528-37.
2007: Lattman Eaton E; Hol Wim G J; Arnold Eddy
Drug design for the third world: a conference report.
Proteins 2007;66(3):509-12.
2007: Wisedchaisri Goragot; Chou C James; Wu Meiting; Roach Claudia; Rice Adrian E; Holmes Randall K; Beeson Craig; Hol Wim G J
Crystal structures, metal activation, and DNA-binding properties of two-domain IdeR from Mycobacterium tuberculosis.
Biochemistry 2007;46(2):436-47.
2007: Camberg Jodi L; Johnson Tanya L; Patrick Marcella; Abendroth Jan; Hol Wim G J; Sandkvist Maria
Synergistic stimulation of EpsE ATP hydrolysis by EpsL and acidic phospholipids.
The EMBO journal 2007;26(1):19-27.
2006: Bosch Jürgen; Robien Mark A; Mehlin Christopher; Boni Erica; Riechers Aaron; Buckner Frederick S; Van Voorhis Wesley C; Myler Peter J; Worthey Elizabeth A; DeTitta George; Luft Joseph R; Lauricella Angela; Gulde Stacey; Anderson Lori A; Kalyuzhniy Oleksandr; Neely Helen M; Ross Jenni; Earnest Thomas N; Soltis Michael; Schoenfeld Lori; Zucker Frank; Merritt Ethan A; Fan Erkang; Verlinde Christophe L M J; Hol Wim G J
Using fragment cocktail crystallography to assist inhibitor design of Trypanosoma brucei nucleoside 2-deoxyribosyltransferase.
Journal of medicinal chemistry 2006;49(20):5939-46.
2006: Korotkov Konstantin V; Krumm Brian; Bagdasarian Michael; Hol Wim G J
Structural and functional studies of EpsC, a crucial component of the type 2 secretion system from Vibrio cholerae.
Journal of molecular biology 2006;363(2):311-21.
2006: Mehlin Christopher; Boni Erica; Buckner Frederick S; Engel Linnea; Feist Tiffany; Gelb Michael H; Haji Lutfiyah; Kim David; Liu Colleen; Mueller Natascha; Myler Peter J; Reddy J T; Sampson Joshua N; Subramanian E; Van Voorhis Wesley C; Worthey Elizabeth; Zucker Frank; Hol Wim G J
Heterologous expression of proteins from Plasmodium falciparum: results from 1000 genes.
Molecular and biochemical parasitology 2006;148(2):144-60.
2006: Sampathkumar Parthasarathy; Turley Stewart; Sibley Carol Hopkins; Hol Wim G J
NADP+ expels both the co-factor and a substrate analog from the Mycobacterium tuberculosis ThyX active site: opportunities for anti-bacterial drug design.
Journal of molecular biology 2006;360(1):1-6.
2006: Holmes Margaret A; Buckner Frederick S; Van Voorhis Wesley C; Verlinde Christophe L M J; Mehlin Christopher; Boni Erica; DeTitta George; Luft Joseph; Lauricella Angela; Anderson Lori; Kalyuzhniy Oleksandr; Zucker Frank; Schoenfeld Lori W; Earnest Thomas N; Hol Wim G J; Merritt Ethan A
Structure of ribose 5-phosphate isomerase from Plasmodium falciparum.
Acta crystallographica. Section F, Structural biology and crystallization communications 2006;62(Pt 5):427-31.
2006: Davies Douglas R; Mushtaq Adeel; Interthal Heidrun; Champoux James J; Hol Wim G J
The structure of the transition state of the heterodimeric topoisomerase I of Leishmania donovani as a vanadate complex with nicked DNA.
Journal of molecular biology 2006;357(4):1202-10.
2006: Bosch Jürgen; Turley Stewart; Daly Thomas M; Bogh Stephen M; Villasmil Michelle L; Roach Claudia; Zhou Na; Morrisey Joanne M; Vaidya Akhil B; Bergman Lawrence W; Hol Wim G J
Structure of the MTIP-MyoA complex, a key component of the malaria parasite invasion motor.
Proceedings of the National Academy of Sciences of the United States of America 2006;103(13):4852-7.
2006: Holmes Margaret A; Buckner Frederick S; Van Voorhis Wesley C; Mehlin Christopher; Boni Erica; Earnest Thomas N; DeTitta George; Luft Joseph; Lauricella Angela; Anderson Lori; Kalyuzhniy Oleksandr; Zucker Frank; Schoenfeld Lori W; Hol Wim G J; Merritt Ethan A
Structure of the conserved hypothetical protein MAL13P1.257 from Plasmodium falciparum.
Acta crystallographica. Section F, Structural biology and crystallization communications 2006;62(Pt 3):180-5.
2006: Arakaki Tracy; Le Trong Isolde; Phizicky Eric; Quartley Erin; DeTitta George; Luft Joseph; Lauricella Angela; Anderson Lori; Kalyuzhniy Oleksandr; Worthey Elizabeth; Myler Peter J; Kim David; Baker David; Hol Wim G J; Merritt Ethan A
Structure of Lmaj006129AAA, a hypothetical protein from Leishmania major.
Acta crystallographica. Section F, Structural biology and crystallization communications 2006;62(Pt 3):175-9.
2006: Robien Mark A; Bosch Jürgen; Buckner Frederick S; Van Voorhis Wesley C E; Worthey Elizabeth A; Myler Peter; Mehlin Christopher; Boni Erica E; Kalyuzhniy Oleksandr; Anderson Lori; Lauricella Angela; Gulde Stacy; Luft Joseph R; DeTitta George; Caruthers Jonathan M; Hodgson Keith O; Soltis Michael; Zucker Frank; Verlinde Christophe L M J; Merritt Ethan A; Schoenfeld Lori W; Hol Wim G J
Crystal structure of glyceraldehyde-3-phosphate dehydrogenase from Plasmodium falciparum at 2.25 A resolution reveals intriguing extra electron density in the active site.
Proteins 2006;62(3):570-7.
2006: Johnson Tanya L; Abendroth Jan; Hol Wim G J; Sandkvist Maria
Type II secretion: from structure to function.
FEMS microbiology letters 2006;255(2):175-86.
2006: Caruthers J; Bosch J; Buckner F; Van Voorhis W; Myler P; Worthey E; Mehlin C; Boni E; DeTitta G; Luft J; Lauricella A; Kalyuzhniy O; Anderson L; Zucker F; Soltis M; Hol Wim G J
Structure of a ribulose 5-phosphate 3-epimerase from Plasmodium falciparum.
Proteins 2006;62(2):338-42.
2005: Deng Junpeng; Ernst Nancy Lewis; Turley Stewart; Stuart Kenneth D; Hol Wim G J
Structural basis for UTP specificity of RNA editing TUTases from Trypanosoma brucei.
The EMBO journal 2005;24(23):4007-17.
2005: Wisedchaisri Goragot; Wu Meiting; Rice Adrian E; Roberts David M; Sherman David R; Hol Wim G J
Structures of Mycobacterium tuberculosis DosR and DosR-DNA complex involved in gene activation during adaptation to hypoxic latency.
Journal of molecular biology 2005;354(3):630-41.
2005: Caruthers Jonathan; Zucker Frank; Worthey Elizabeth; Myler Peter J; Buckner Fred; Van Voorhuis Wes; Mehlin Chris; Boni Erica; Feist Tiffany; Luft Joseph; Gulde Stacey; Lauricella Angela; Kaluzhniy Oleksandr; Anderson Lori; Le Trong Isolde; Holmes Margaret A; Earnest Thomas; Soltis Michael; Hodgson Keith O; Hol Wim G J; Merritt Ethan A
Crystal structures and proposed structural/functional classification of three protozoan proteins from the isochorismatase superfamily.
Protein science : a publication of the Protein Society 2005;14(11):2887-94.
2005: Sampathkumar Parthasarathy; Turley Stewart; Ulmer Jonathan E; Rhie Ho Gun; Sibley Carol Hopkins; Hol Wim G J
Structure of the Mycobacterium tuberculosis flavin dependent thymidylate synthase (MtbThyX) at 2.0A resolution.
Journal of molecular biology 2005;352(5):1091-104.
2005: Schnick Claudia; Robien Mark A; Brzozowski Andrzej M; Dodson Eleanor J; Murshudov Garib N; Anderson Lori; Luft Joseph R; Mehlin Chris; Hol Wim G J; Brannigan James A; Wilkinson Anthony J
Structures of Plasmodium falciparum purine nucleoside phosphorylase complexed with sulfate and its natural substrate inosine.
Acta crystallographica. Section D, Biological crystallography 2005;61(Pt 9):1245-54.
2005: O'Neal Claire J; Jobling Michael G; Holmes Randall K; Hol Wim G J
Structural basis for the activation of cholera toxin by human ARF6-GTP.
Science (New York, N.Y.) 2005;309(5737):1093-6.
2005: Abendroth Jan; Murphy Paul; Sandkvist Maria; Bagdasarian Michael; Hol Wim G J
The X-ray structure of the type II secretion system complex formed by the N-terminal domain of EpsE and the cytoplasmic domain of EpsL of Vibrio cholerae.
Journal of molecular biology 2005;348(4):845-55.
2005: Liu Jiyun; Zhang Zhongsheng; Tan Xiaojian; Hol Wim G J; Verlinde Christophe L M J; Fan Erkang
Protein heterodimerization through ligand-bridged multivalent pre-organization: enhancing ligand binding toward both protein targets.
Journal of the American Chemical Society 2005;127(7):2044-5.
2004: Chou C James; Wisedchaisri Goragot; Monfeli Ryan R; Oram Diana M; Holmes Randall K; Hol Wim G J; Beeson Craig
Functional studies of the Mycobacterium tuberculosis iron-dependent regulator.
The Journal of biological chemistry 2004;279(51):53554-61.
2004: Davies Douglas R; Hol Wim G J
The power of vanadate in crystallographic investigations of phosphoryl transfer enzymes.
FEBS letters 2004;577(3):315-21.
2004: Moyersoen Juliette; Choe Jungwoo; Fan Erkang; Hol Wim G J; Michels Paul A M
Biogenesis of peroxisomes and glycosomes: trypanosomatid glycosome assembly is a promising new drug target.
FEMS microbiology reviews 2004;28(5):603-43.
2004: Abendroth Jan; Bagdasarian Michael; Sandkvist Maria; Hol Wim G J
The structure of the cytoplasmic domain of EpsL, an inner membrane component of the type II secretion system of Vibrio cholerae: an unusual member of the actin-like ATPase superfamily.
Journal of molecular biology 2004;344(3):619-33.
2004: Fan Erkang; O'Neal Claire J; Mitchell Daniel D; Robien Mark A; Zhang Zhongsheng; Pickens Jason C; Tan Xiao-Jian; Korotkov Konstantin; Roach Claudia; Krumm Brian; Verlinde Christophe L M J; Merritt Ethan A; Hol Wim G J
Structural biology and structure-based inhibitor design of cholera toxin and heat-labile enterotoxin.
International journal of medical microbiology : IJMM 2004;294(4):217-23.
2004: Zhang Zhongsheng; Liu Jiyun; Verlinde Christophe L M J; Hol Wim G J; Fan Erkang
Large cyclic peptides as cores of multivalent ligands: application to inhibitors of receptor binding by cholera toxin.
The Journal of organic chemistry 2004;69(22):7737-40.
2004: Deng Junpeng; Schnaufer Achim; Salavati Reza; Stuart Kenneth D; Hol Wim G J
High resolution crystal structure of a key editosome enzyme from Trypanosoma brucei: RNA editing ligase 1.
Journal of molecular biology 2004;343(3):601-13.
2004: Pickens Jason C; Mitchell Daniel D; Liu Jiyun; Tan Xiaojian; Zhang Zhongsheng; Verlinde Christophe L M J; Hol Wim G J; Fan Erkang
Nonspanning bivalent ligands as improved surface receptor binding inhibitors of the cholera toxin B pentamer.
Chemistry & biology 2004;11(9):1205-15.
2004: Wisedchaisri Goragot; Holmes Randall K; Hol Wim G J
Crystal structure of an IdeR-DNA complex reveals a conformational change in activated IdeR for base-specific interactions.
Journal of molecular biology 2004;342(4):1155-69.
2004: Sastry M S R; Quigley Paulene M; Hol Wim G J; Baneyx François
The linker-loop region of Escherichia coli chaperone Hsp31 functions as a gate that modulates high-affinity substrate binding at elevated temperatures.
Proceedings of the National Academy of Sciences of the United States of America 2004;101(23):8587-92.
2004: Roberts David M; Liao Reiling P; Wisedchaisri Goragot; Hol Wim G J; Sherman David R
Two sensor kinases contribute to the hypoxic response of Mycobacterium tuberculosis.
The Journal of biological chemistry 2004;279(22):23082-7.
2004: Zhang Zhongsheng; Pickens Jason C; Hol Wim G J; Fan Erkang
Solution- and solid-phase syntheses of guanidine-bridged, water-soluble linkers for multivalent ligand design.
Organic letters 2004;6(9):1377-80.
2004: Abendroth Jan; Rice Adrian E; McLuskey Karen; Bagdasarian Michael; Hol Wim G J
The crystal structure of the periplasmic domain of the type II secretion system protein EpsM from Vibrio cholerae: the simplest version of the ferredoxin fold.
Journal of molecular biology 2004;338(3):585-96.
2004: O'Neal Claire J; Amaya Edward I; Jobling Michael G; Holmes Randall K; Hol Wim G J
Crystal structures of an intrinsically active cholera toxin mutant yield insight into the toxin activation mechanism.
Biochemistry 2004;43(13):3772-82.
2004: Robien Mark A; Nguyen Kiet T; Kumar Abhinav; Hirsh Irwin; Turley Stewart; Pei Dehua; Hol Wim G J
An improved crystal form of Plasmodium falciparum peptide deformylase.
Protein science : a publication of the Protein Society 2004;13(4):1155-63.
2004: Mitchell Daniel D; Pickens Jason C; Korotkov Konstantin; Fan Erkang; Hol Wim G J
3,5-Substituted phenyl galactosides as leads in designing effective cholera toxin antagonists; synthesis and crystallographic studies.
Bioorganic & medicinal chemistry 2004;12(5):907-20.
2004: Davies Douglas R; Interthal Heidrun; Champoux James J; Hol Wim G J
Explorations of peptide and oligonucleotide binding sites of tyrosyl-DNA phosphodiesterase using vanadate complexes.
Journal of medicinal chemistry 2004;47(4):829-37.
2004: Yeh Joanne I; Charrier Véronique; Paulo Joao; Hou Lihui; Darbon Emmanuelle; Claiborne Al; Hol Wim G J; Deutscher Josef
Structures of enterococcal glycerol kinase in the absence and presence of glycerol: correlation of conformation to substrate binding and a mechanism of activation by phosphorylation.
Biochemistry 2004;43(2):362-73.
2004: Quigley Paulene M; Korotkov Konstantin; Baneyx François; Hol Wim G J
A new native EcHsp31 structure suggests a key role of structural flexibility for chaperone function.
Protein science : a publication of the Protein Society 2004;13(1):269-77.
2004: Deng Junpeng; Davies Douglas R; Wisedchaisri Goragot; Wu Meiting; Hol Wim G J; Mehlin Christopher
An improved protocol for rapid freezing of protein samples for long-term storage.
Acta crystallographica. Section D, Biological crystallography 2004;60(Pt 1):203-4.
2004: Interthal Heidrun; Quigley Paulene M; Hol Wim G J; Champoux James J
The role of lysine 532 in the catalytic mechanism of human topoisomerase I.
The Journal of biological chemistry 2004;279(4):2984-92.
2003: Buscaglia Carlos A; Coppens Isabelle; Hol Wim G J; Nussenzweig Victor
Sites of interaction between aldolase and thrombospondin-related anonymous protein in plasmodium.
Molecular biology of the cell 2003;14(12):4947-57.
2003: Robien Mark A; Krumm Brian E; Sandkvist Maria; Hol Wim G J
Crystal structure of the extracellular protein secretion NTPase EpsE of Vibrio cholerae.
Journal of molecular biology 2003;333(3):657-74.
2003: Choe Jungwoo; Moyersoen Juliette; Roach Claudia; Carter Tyan L; Fan Erkang; Michels Paul A M; Hol Wim G J
Analysis of the sequence motifs responsible for the interactions of peroxins 14 and 5, which are involved in glycosome biogenesis in Trypanosoma brucei.
Biochemistry 2003;42(37):10915-22.
2003: Hu Xubo; Nguyen Kiet T; Verlinde Christophe L M J; Hol Wim G J; Pei Dehua
Structure-based design of a macrocyclic inhibitor for peptide deformylase.
Journal of medicinal chemistry 2003;46(18):3771-4.
2003: Tinker Juliette K; Erbe Jarrod L; Hol Wim G J; Holmes Randall K
Cholera holotoxin assembly requires a hydrophobic domain at the A-B5 interface: mutational analysis and development of an in vitro assembly system.
Infection and immunity 2003;71(7):4093-101.
2003: Choe Jungwoo; Guerra Daniel; Michels Paul A M; Hol Wim G J
Leishmania mexicana glycerol-3-phosphate dehydrogenase showed conformational changes upon binding a bi-substrate adduct.
Journal of molecular biology 2003;329(2):335-49.
2003: Moyersoen Juliette; Choe Jungwoo; Kumar Abhinav; Voncken Frank G J; Hol Wim G J; Michels Paul A M
Characterization of Trypanosoma brucei PEX14 and its role in the import of glycosomal matrix proteins.
European journal of biochemistry / FEBS 2003;270(9):2059-67.
2003: Quigley Paulene M; Korotkov Konstantin; Baneyx Francois; Hol Wim G J
The 1.6-A crystal structure of the class of chaperones represented by Escherichia coli Hsp31 reveals a putative catalytic triad.
Proceedings of the National Academy of Sciences of the United States of America 2003;100(6):3137-42.
2003: Davies Douglas R; Interthal Heidrun; Champoux James J; Hol Wim G J
Crystal structure of a transition state mimic for Tdp1 assembled from vanadate, DNA, and a topoisomerase I-derived peptide.
Chemistry & biology 2003;10(2):139-47.
2002: Davies Douglas R; Interthal Heidrun; Champoux James J; Hol Wim G J
Insights into substrate binding and catalytic mechanism of human tyrosyl-DNA phosphodiesterase (Tdp1) from vanadate and tungstate-inhibited structures.
Journal of molecular biology 2002;324(5):917-32.
2002: Choe Jungwoo; Suresh Stephen; Wisedchaisri Goragot; Kennedy Kevin J; Gelb Michael H; Hol Wim G J
Anomalous differences of light elements in determining precise binding modes of ligands to glycerol-3-phosphate dehydrogenase.
Chemistry & biology 2002;9(11):1189-97.
2002: Zhang Zhongsheng; Merritt Ethan A; Ahn Misol; Roach Claudia; Hou Zheng; Verlinde Christophe L M J; Hol Wim G J; Fan Erkang
Solution and crystallographic studies of branched multivalent ligands that inhibit the receptor-binding of cholera toxin.
Journal of the American Chemical Society 2002;124(44):12991-8.
2002: Martinez Sergio E; Wu Albert Y; Glavas Natalie A; Tang Xiao-Bo; Turley Stewart; Hol Wim G J; Beavo Joseph A
The two GAF domains in phosphodiesterase 2A have distinct roles in dimerization and in cGMP binding.
Proceedings of the National Academy of Sciences of the United States of America 2002;99(20):13260-5.
2002: Martinez Sergio E; Beavo Joseph A; Hol Wim G J
GAF domains: two-billion-year-old molecular switches that bind cyclic nucleotides.
Molecular interventions 2002;2(5):317-23.
2002: Merritt Ethan A; Zhang Zhongsheng; Pickens Jason C; Ahn Misol; Hol Wim G J; Fan Erkang
Characterization and crystal structure of a high-affinity pentavalent receptor-binding inhibitor for cholera toxin and E. coli heat-labile enterotoxin.
Journal of the American Chemical Society 2002;124(30):8818-24.
2002: Gelb Michael H; Hol Wim G J
Parasitology. Drugs to combat tropical protozoan parasites.
Science (New York, N.Y.) 2002;297(5580):343-4.
2002: Kumar Abhinav; Nguyen Kiet T; Srivathsan Sumant; Ornstein Brad; Turley Stewart; Hirsh Irwin; Pei Dehua; Hol Wim G J
Crystals of peptide deformylase from Plasmodium falciparum reveal critical characteristics of the active site for drug design.
Structure (London, England : 1993) 2002;10(3):357-67.
2002: Pickens Jason C; Merritt Ethan A; Ahn Misol; Verlinde Christophe L M J; Hol Wim G J; Fan Erkang
Anchor-based design of improved cholera toxin and E. coli heat-labile enterotoxin receptor binding antagonists that display multiple binding modes.
Chemistry & biology 2002;9(2):215-24.
2002: Davies Douglas R; Interthal Heidrun; Champoux James J; Hol Wim G J
The crystal structure of human tyrosyl-DNA phosphodiesterase, Tdp1.
Structure (London, England : 1993) 2002;10(2):237-48.
2002: Kim Youngsoo; Kim Sanggu; Earnest Thomas N; Hol Wim G J
Precursor structure of cephalosporin acylase. Insights into autoproteolytic activation in a new N-terminal hydrolase family.
The Journal of biological chemistry 2002;277(4):2823-9.
2001: Kim Y; Hol W G
Structure of cephalosporin acylase in complex with glutaryl-7-aminocephalosporanic acid and glutarate: insight into the basis of its substrate specificity.
Chemistry & biology 2001;8(12):1253-64.
2001: Aman A T; Fraser S; Merritt E A; Rodigherio C; Kenny M; Ahn M; Hol W G; Williams N A; Lencer W I; Hirst T R
A mutant cholera toxin B subunit that binds GM1- ganglioside but lacks immunomodulatory or toxic activity.
Proceedings of the National Academy of Sciences of the United States of America 2001;98(15):8536-41.
2001: Bressi J C; Verlinde C L; Aronov A M; Shaw M L; Shin S S; Nguyen L N; Suresh S; Buckner F S; Van Voorhis W C; Kuntz I D; Hol W G; Gelb M H
Adenosine analogues as selective inhibitors of glyceraldehyde-3-phosphate dehydrogenase of Trypanosomatidae via structure-based drug design.
Journal of medicinal chemistry 2001;44(13):2080-93.
2001: Suresh S; Bressi J C; Kennedy K J; Verlinde C L; Gelb M H; Hol W G
Conformational changes in Leishmania mexicana glyceraldehyde-3-phosphate dehydrogenase induced by designed inhibitors.
Journal of molecular biology 2001;309(2):423-35.
2001: Pohl E; Goranson-Siekierke J; Choi M K; Roosild T; Holmes R K; Hol W G
Structures of three diphtheria toxin repressor (DtxR) variants with decreased repressor activity.
Acta crystallographica. Section D, Biological crystallography 2001;57(Pt 5):619-27.
2001: Ghosh A K; Hol W G; Fan E
Solid-phase synthesis of N-acyl-N'-alkyl/aryl disubstituted guanidines.
The Journal of organic chemistry 2001;66(6):2161-4.
2001: Kumar A; Roach C; Hirsh I S; Turley S; deWalque S; Michels P A; Hol W G
An unexpected extended conformation for the third TPR motif of the peroxin PEX5 from Trypanosoma brucei.
Journal of molecular biology 2001;307(1):271-82.
2001: Verlinde C L; Hannaert V; Blonski C; Willson M; Périé J J; Fothergill-Gilmore L A; Opperdoes F R; Gelb M H; Hol W G; Michels P A
Glycolysis as a target for the design of new anti-trypanosome drugs.
Drug resistance updates : reviews and commentaries in antimicrobial and anticancer chemotherapy 2001;4(1):50-65.
2001: Fan E; Merritt E A; Zhang Z; Pickens J C; Roach C; Ahn M; Hol W G
Exploration of the GM1 receptor-binding site of heat-labile enterotoxin and cholera toxin by phenyl-ring-containing galactose derivatives.
Acta crystallographica. Section D, Biological crystallography 2001;57(Pt 2):201-12.
2001: Feese M D; Ingason B P; Goranson-Siekierke J; Holmes R K; Hol W G
Crystal structure of the iron-dependent regulator from Mycobacterium tuberculosis at 2.0-A resolution reveals the Src homology domain 3-like fold and metal binding function of the third domain.
The Journal of biological chemistry 2001;276(8):5959-66.
2000: Fan E; Merritt E A; Verlinde C L; Hol W G
AB(5) toxins: structures and inhibitor design.
Current opinion in structural biology 2000;10(6):680-6.
2000: Hol W G
Structural genomics for science and society.
Nature structural biology 2000;7 Suppl():964-6.
2000: Bressi J C; Choe J; Hough M T; Buckner F S; Van Voorhis W C; Verlinde C L; Hol W G; Gelb M H
Adenosine analogues as inhibitors of Trypanosoma brucei phosphoglycerate kinase: elucidation of a novel binding mode for a 2-amino-N(6)-substituted adenosine.
Journal of medicinal chemistry 2000;43(22):4135-50.
2000: Kim Y; Yoon K; Khang Y; Turley S; Hol W G
The 2.0 A crystal structure of cephalosporin acylase.
Structure (London, England : 1993) 2000;8(10):1059-68.
2000: Baca A M; Sirawaraporn R; Turley S; Sirawaraporn W; Hol W G
Crystal structure of Mycobacterium tuberculosis 7,8-dihydropteroate synthase in complex with pterin monophosphate: new insight into the enzymatic mechanism and sulfa-drug action.
Journal of molecular biology 2000;302(5):1193-212.
2000: Minke W E; Pickens J; Merritt E A; Fan E; Verlinde C L; Hol W G
Structure of m-carboxyphenyl-alpha-D-galactopyranoside complexed to heat-labile enterotoxin at 1.3 A resolution: surprising variations in ligand-binding modes.
Acta crystallographica. Section D, Biological crystallography 2000;56(Pt 7):795-804.
2000: Chudzik D M; Michels P A; de Walque S; Hol W G
Structures of type 2 peroxisomal targeting signals in two trypanosomatid aldolases.
Journal of molecular biology 2000;300(4):697-707.
2000: Redinbo M R; Champoux J J; Hol W G
Novel insights into catalytic mechanism from a crystal structure of human topoisomerase I in complex with DNA.
Biochemistry 2000;39(23):6832-40.
2000: Suresh S; Turley S; Opperdoes F R; Michels P A; Hol W G
A potential target enzyme for trypanocidal drugs revealed by the crystal structure of NAD-dependent glycerol-3-phosphate dehydrogenase from Leishmania mexicana.
Structure (London, England : 1993) 2000;8(5):541-52.
2000: AEvarsson A; Chuang J L; Wynn R M; Turley S; Chuang D T; Hol W G
Crystal structure of human branched-chain alpha-ketoacid dehydrogenase and the molecular basis of multienzyme complex deficiency in maple syrup urine disease.
Structure (London, England : 1993) 2000;8(3):277-91.
2000: Baca A M; Hol W G
Overcoming codon bias: a method for high-level overexpression of Plasmodium and other AT-rich parasite genes in Escherichia coli.
International journal for parasitology 2000;30(2):113-8.
2000: Li R; Sirawaraporn R; Chitnumsub P; Sirawaraporn W; Wooden J; Athappilly F; Turley S; Hol W G
Three-dimensional structure of M. tuberculosis dihydrofolate reductase reveals opportunities for the design of novel tuberculosis drugs.
Journal of molecular biology 2000;295(2):307-23.
1999: Minke W E; Hong F; Verlinde C L; Hol W G; Fan E
Using a galactose library for exploration of a novel hydrophobic pocket in the receptor binding site of the Escherichia coli heat-labile enterotoxin.
The Journal of biological chemistry 1999;274(47):33469-73.
1999: Redinbo M R; Stewart L; Champoux J J; Hol W G
Structural flexibility in human topoisomerase I revealed in multiple non-isomorphous crystal structures.
Journal of molecular biology 1999;292(3):685-96.
1999: Pohl E; Holmes R K; Hol W G
Crystal structure of a cobalt-activated diphtheria toxin repressor-DNA complex reveals a metal-binding SH3-like domain.
Journal of molecular biology 1999;292(3):653-67.
1999: Diller D J; Redinbo M R; Pohl E; Hol W G
A database method for automated map interpretation in protein crystallography.
Proteins 1999;36(4):526-41.
1999: Diller D J; Pohl E; Redinbo M R; Hovey B T; Hol W G
A rapid method for positioning small flexible molecules, nucleic acids, and large protein fragments in experimental electron density maps.
Proteins 1999;36(4):512-25.
1999: Aevarsson A; Seger K; Turley S; Sokatch J R; Hol W G
Crystal structure of 2-oxoisovalerate and dehydrogenase and the architecture of 2-oxo acid dehydrogenase multienzyme complexes.
Nature structural biology 1999;6(8):785-92.
1999: Minke W E; Diller D J; Hol W G; Verlinde C L
The role of waters in docking strategies with incremental flexibility for carbohydrate derivatives: heat-labile enterotoxin, a multivalent test case.
Journal of medicinal chemistry 1999;42(10):1778-88.
1999: Minke W E; Roach C; Hol W G; Verlinde C L
Structure-based exploration of the ganglioside GM1 binding sites of Escherichia coli heat-labile enterotoxin and cholera toxin for the discovery of receptor antagonists.
Biochemistry 1999;38(18):5684-92.
1999: Aronov A M; Suresh S; Buckner F S; Van Voorhis W C; Verlinde C L; Opperdoes F R; Hol W G; Gelb M H
Structure-based design of submicromolar, biologically active inhibitors of trypanosomatid glyceraldehyde-3-phosphate dehydrogenase.
Proceedings of the National Academy of Sciences of the United States of America 1999;96(8):4273-8.
1999: Goranson-Siekierke J; Pohl E; Hol W G; Holmes R K
Anion-coordinating residues at binding site 1 are essential for the biological activity of the diphtheria toxin repressor.
Infection and immunity 1999;67(4):1806-11.
1999: Diller D J; Hol W G
An accurate numerical model for calculating the equilibration rate of a hanging-drop experiment.
Acta crystallographica. Section D, Biological crystallography 1999;55(Pt 3):656-63.
1999: Redinbo M R; Champoux J J; Hol W G
Structural insights into the function of type IB topoisomerases.
Current opinion in structural biology 1999;9(1):29-36.
1999: Izard T; Aevarsson A; Allen M D; Westphal A H; Perham R N; de Kok A; Hol W G
Principles of quasi-equivalence and Euclidean geometry govern the assembly of cubic and dodecahedral cores of pyruvate dehydrogenase complexes.
Proceedings of the National Academy of Sciences of the United States of America 1999;96(4):1240-5.
1999: van den Akker F; Hol W G
Difference density quality (DDQ): a method to assess the global and local correctness of macromolecular crystal structures.
Acta crystallographica. Section D, Biological crystallography 1999;55(Pt 1):206-18.
1999: Hovey B T; Verlinde C L; Merritt E A; Hol W G
Structure-based discovery of a pore-binding ligand: towards assembly inhibitors for cholera and related AB5 toxins.
Journal of molecular biology 1999;285(3):1169-78.
1999: Pohl E; Holmes R K; Hol W G
Crystal structure of the iron-dependent regulator (IdeR) from Mycobacterium tuberculosis shows both metal binding sites fully occupied.
Journal of molecular biology 1999;285(3):1145-56.
1998: Feil I K; Platas A A; van den Akker F; Reddy R; Merritt E A; Storm D R; Hol W G
Stepwise transplantation of an active site loop between heat-labile enterotoxins LT-II and LT-I and characterization of the obtained hybrid toxins.
Protein engineering 1998;11(11):1103-9.
1998: Aronov A M; Verlinde C L; Hol W G; Gelb M H
Selective tight binding inhibitors of trypanosomal glyceraldehyde-3-phosphate dehydrogenase via structure-based drug design.
Journal of medicinal chemistry 1998;41(24):4790-9.
1998: Merritt E A; Kuhn P; Sarfaty S; Erbe J L; Holmes R K; Hol W G
The 1.25 A resolution refinement of the cholera toxin B-pentamer: evidence of peptide backbone strain at the receptor-binding site.
Journal of molecular biology 1998;282(5):1043-59.
1998: Pohl E; Holmes R K; Hol W G
Motion of the DNA-binding domain with respect to the core of the diphtheria toxin repressor (DtxR) revealed in the crystal structures of apo- and holo-DtxR.
The Journal of biological chemistry 1998;273(35):22420-7.
1998: Bernstein B E; Williams D M; Bressi J C; Kuhn P; Gelb M H; Blackburn G M; Hol W G
A bisubstrate analog induces unexpected conformational changes in phosphoglycerate kinase from Trypanosoma brucei.
Journal of molecular biology 1998;279(5):1137-48.
1998: Yeh J I; Hol W G
A flash-annealing technique to improve diffraction limits and lower mosaicity in crystals of glycerol kinase.
Acta crystallographica. Section D, Biological crystallography 1998;54(Pt 3):479-80.
1998: Kim H; Hol W G
Crystal structure of Leishmania mexicana glycosomal glyceraldehyde-3-phosphate dehydrogenase in a new crystal form confirms the putative physiological active site structure.
Journal of molecular biology 1998;278(1):5-11.
1998: Bernstein B E; Hol W G
Crystal structures of substrates and products bound to the phosphoglycerate kinase active site reveal the catalytic mechanism.
Biochemistry 1998;37(13):4429-36.
1998: Stewart L; Redinbo M R; Qiu X; Hol W G; Champoux J J
A model for the mechanism of human topoisomerase I.
Science (New York, N.Y.) 1998;279(5356):1534-41.
1998: Redinbo M R; Stewart L; Kuhn P; Champoux J J; Hol W G
Crystal structures of human topoisomerase I in covalent and noncovalent complexes with DNA.
Science (New York, N.Y.) 1998;279(5356):1504-13.
1998: Bernstein B E; Michels P A; Kim H; Petra P H; Hol W G
The importance of dynamic light scattering in obtaining multiple crystal forms of Trypanosoma brucei PGK.
Protein science : a publication of the Protein Society 1998;7(2):504-7.
1998: Rudenko G; Bonten E; Hol W G; d'Azzo A
The atomic model of the human protective protein/cathepsin A suggests a structural basis for galactosialidosis.
Proceedings of the National Academy of Sciences of the United States of America 1998;95(2):621-5.
1997: van den Akker F; Pizza M; Rappuoli R; Hol W G
Crystal structure of a non-toxic mutant of heat-labile enterotoxin, which is a potent mucosal adjuvant.
Protein science : a publication of the Protein Society 1997;6(12):2650-4.
1997: van den Akker F; Feil I K; Roach C; Platas A A; Merritt E A; Hol W G
Crystal structure of heat-labile enterotoxin from Escherichia coli with increased thermostability introduced by an engineered disulfide bond in the A subunit.
Protein science : a publication of the Protein Society 1997;6(12):2644-9.
1997: Bernstein B E; Hol W G
Probing the limits of the molecular replacement method: the case of Trypanosoma brucei phosphoglycerate kinase.
Acta crystallographica. Section D, Biological crystallography 1997;53(Pt 6):756-64.
1997: Merritt E A; Sarfaty S; Feil I K; Hol W G
Structural foundation for the design of receptor antagonists targeting Escherichia coli heat-labile enterotoxin.
Structure (London, England : 1993) 1997;5(11):1485-99.
1997: Merritt E A; Sarfaty S; Jobling M G; Chang T; Holmes R K; Hirst T R; Hol W G
Structural studies of receptor binding by cholera toxin mutants.
Protein science : a publication of the Protein Society 1997;6(7):1516-28.
1997: Pohl E; Qui X; Must L M; Holmes R K; Hol W G
Comparison of high-resolution structures of the diphtheria toxin repressor in complex with cobalt and zinc at the cation-anion binding site.
Protein science : a publication of the Protein Society 1997;6(5):1114-8.
1997: Izard T; Sarfaty S; Westphal A; de Kok A; Hol W G
Improvement of diffraction quality upon rehydration of dehydrated icosahedral Enterococcus faecalis pyruvate dehydrogenase core crystals.
Protein science : a publication of the Protein Society 1997;6(4):913-5.
1997: Bernstein B E; Michels P A; Hol W G
Synergistic effects of substrate-induced conformational changes in phosphoglycerate kinase activation.
Nature 1997;385(6613):275-8.
1996: de Haan L; Verweij W R; Feil I K; Lijnema T H; Hol W G; Agsteribbe E; Wilschut J
Mutants of the Escherichia coli heat-labile enterotoxin with reduced ADP-ribosylation activity or no activity retain the immunogenic properties of the native holotoxin.
Infection and immunity 1996;64(12):5413-6.
1996: Hazes B; Magnus K A; Kalk K H; Bonaventura C; Hol W G
Nitrate binding to Limulus polyphemus subunit type II hemocyanin and its functional implications.
Journal of molecular biology 1996;262(4):532-41.
1996: Rudenko G; Bonten E; d'Azzo A; Hol W G
Structure determination of the human protective protein: twofold averaging reveals the three-dimensional structure of a domain which was entirely absent in the initial model.
Acta crystallographica. Section D, Biological crystallography 1996;52(Pt 5):923-36.
1996: Qiu X; Pohl E; Holmes R K; Hol W G
High-resolution structure of the diphtheria toxin repressor complexed with cobalt and manganese reveals an SH3-like third domain and suggests a possible role of phosphate as co-corepressor.
Biochemistry 1996;35(38):12292-302.
1996: Yeh J I; Claiborne A; Hol W G
Structure of the native cysteine-sulfenic acid redox center of enterococcal NADH peroxidase refined at 2.8 A resolution.
Biochemistry 1996;35(31):9951-7.
1996: van den Akker F; Sarfaty S; Twiddy E M; Connell T D; Holmes R K; Hol W G
Crystal structure of a new heat-labile enterotoxin, LT-IIb.
Structure (London, England : 1993) 1996;4(6):665-78.
1996: van den Akker F; Steensma E; Hol W G
Tumor marker disaccharide D-Gal-beta 1, 3-GalNAc complexed to heat-labile enterotoxin from Escherichia coli.
Protein science : a publication of the Protein Society 1996;5(6):1184-8.
1996: Feil I K; Reddy R; de Haan L; Merritt E A; van den Akker F; Storm D R; Hol W G
Protein engineering studies of A-chain loop 47-56 of Escherichia coli heat-labile enterotoxin point to a prominent role of this loop for cytotoxicity.
Molecular microbiology 1996;20(4):823-32.
1996: Mainfroid V; Mande S C; Hol W G; Martial J A; Goraj K
Stabilization of human triosephosphate isomerase by improvement of the stability of individual alpha-helices in dimeric as well as monomeric forms of the protein.
Biochemistry 1996;35(13):4110-7.
1996: Mande S S; Sarfaty S; Allen M D; Perham R N; Hol W G
Protein-protein interactions in the pyruvate dehydrogenase multienzyme complex: dihydrolipoamide dehydrogenase complexed with the binding domain of dihydrolipoamide acetyltransferase.
Structure (London, England : 1993) 1996;4(3):277-86.
1996: Mainfroid V; Terpstra P; Beauregard M; Frère J M; Mande S C; Hol W G; Martial J A; Goraj K
Three hTIM mutants that provide new insights on why TIM is a dimer.
Journal of molecular biology 1996;257(2):441-56.
1996: Mande S C; Mehra V; Bloom B R; Hol W G
Structure of the heat shock protein chaperonin-10 of Mycobacterium leprae.
Science (New York, N.Y.) 1996;271(5246):203-7.
1996: Teplyakov A; Gros P; Hol W G
Crystallographic study of eglin-C binding to thermitase.
Advances in experimental medicine and biology 1996;379():5-9.
1995: Delboni L F; Mande S C; Rentier-Delrue F; Mainfroid V; Turley S; Vellieux F M; Martial J A; Hol W G
Crystal structure of recombinant triosephosphate isomerase from Bacillus stearothermophilus. An analysis of potential thermostability factors in six isomerases with known three-dimensional structures points to the importance of hydrophobic interactions.
Protein science : a publication of the Protein Society 1995;4(12):2594-604.
1995: Rudenko G; Bonten E; d'Azzo A; Hol W G
Three-dimensional structure of the human 'protective protein': structure of the precursor form suggests a complex activation mechanism.
Structure (London, England : 1993) 1995;3(11):1249-59.
1995: Kim H; Feil I K; Verlinde C L; Petra P H; Hol W G
Crystal structure of glycosomal glyceraldehyde-3-phosphate dehydrogenase from Leishmania mexicana: implications for structure-based drug design and a new position for the inorganic phosphate binding site.
Biochemistry 1995;34(46):14975-86.
1995: van den Akker F; Merritt E A; Pizza M; Domenighini M; Rappuoli R; Hol W G
The Arg7Lys mutant of heat-labile enterotoxin exhibits great flexibility of active site loop 47-56 of the A subunit.
Biochemistry 1995;34(35):10996-1004.
1995: Van Calenbergh S; Verlinde C L; Soenens J; De Bruyn A; Callens M; Blaton N M; Peeters O M; Rozenski J; Hol W G; Herdewijn P
Synthesis and structure-activity relationships of analogs of 2'-deoxy-2'-(3-methoxybenzamido)adenosine, a selective inhibitor of trypanosomal glycosomal glyceraldehyde-3-phosphate dehydrogenase.
Journal of medicinal chemistry 1995;38(19):3838-49.
1995: Vellieux F M; Hajdu J; Hol W G
Refined 3.2 A structure of glycosomal holo glyceraldehyde phosphate dehydrogenase from Trypanosoma brucei brucei.
Acta crystallographica. Section D, Biological crystallography 1995;51(Pt 4):575-89.
1995: Merritt E A; Sarfaty S; Chang T T; Palmer L M; Jobling M G; Holmes R K; Hol W G
Surprising leads for a cholera toxin receptor-binding antagonist: crystallographic studies of CTB mutants.
Structure (London, England : 1993) 1995;3(6):561-70.
1995: Mande S S; Parsonage D; Claiborne A; Hol W G
Crystallographic analyses of NADH peroxidase Cys42Ala and Cys42Ser mutants: active site structures, mechanistic implications, and an unusual environment of Arg 303.
Biochemistry 1995;34(21):6985-92.
1995: Shoham M; Scherf T; Anglister J; Levitt M; Merritt E A; Hol W G
Structural diversity in a conserved cholera toxin epitope involved in ganglioside binding.
Protein science : a publication of the Protein Society 1995;4(5):841-8.
1995: Merritt E A; Sarfaty S; Pizza M; Domenighini M; Rappuoli R; Hol W G
Mutation of a buried residue causes loss of activity but no conformational change in the heat-labile enterotoxin of Escherichia coli.
Nature structural biology 1995;2(4):269-72.
1995: Miller H; Mande S S; Parsonage D; Sarfaty S H; Hol W G; Claiborne A
An L40C mutation converts the cysteine-sulfenic acid redox center in enterococcal NADH peroxidase to a disulfide.
Biochemistry 1995;34(15):5180-90.
1995: Hendle J; Mattevi A; Westphal A H; Spee J; de Kok A; Teplyakov A; Hol W G
Crystallographic and enzymatic investigations on the role of Ser558, His610, and Asn614 in the catalytic mechanism of Azotobacter vinelandii dihydrolipoamide acetyltransferase (E2p).
Biochemistry 1995;34(13):4287-98.
1995: Merritt E A; Hol W G
AB5 toxins.
Current opinion in structural biology 1995;5(2):165-71.
1995: Qiu X; Verlinde C L; Zhang S; Schmitt M P; Holmes R K; Hol W G
Three-dimensional structure of the diphtheria toxin repressor in complex with divalent cation co-repressors.
Structure (London, England : 1993) 1995;3(1):87-100.
1994: Pizza M; Fontana M R; Giuliani M M; Domenighini M; Magagnoli C; Giannelli V; Nucci D; Hol W; Manetti R; Rappuoli R
A genetically detoxified derivative of heat-labile Escherichia coli enterotoxin induces neutralizing antibodies against the A subunit.
The Journal of experimental medicine 1994;180(6):2147-53.
1994: Verlinde C L; Callens M; Van Calenbergh S; Van Aerschot A; Herdewijn P; Hannaert V; Michels P A; Opperdoes F R; Hol W G
Selective inhibition of trypanosomal glyceraldehyde-3-phosphate dehydrogenase by protein structure-based design: toward new drugs for the treatment of sleeping sickness.
Journal of medicinal chemistry 1994;37(21):3605-13.
1994: Verlinde C L; Merritt E A; Van den Akker F; Kim H; Feil I; Delboni L F; Mande S C; Sarfaty S; Petra P H; Hol W G
Protein crystallography and infectious diseases.
Protein science : a publication of the Protein Society 1994;3(10):1670-86.
1994: Pizza M; Domenighini M; Hol W; Giannelli V; Fontana M R; Giuliani M M; Magagnoli C; Peppoloni S; Manetti R; Rappuoli R
Probing the structure-activity relationship of Escherichia coli LT-A by site-directed mutagenesis.
Molecular microbiology 1994;14(1):51-60.
1994: Merritt E A; Sixma T K; Kalk K H; van Zanten B A; Hol W G
Galactose-binding site in Escherichia coli heat-labile enterotoxin (LT) and cholera toxin (CT).
Molecular microbiology 1994;13(4):745-53.
1994: Magnus K A; Hazes B; Ton-That H; Bonaventura C; Bonaventura J; Hol W G
Crystallographic analysis of oxygenated and deoxygenated states of arthropod hemocyanin shows unusual differences.
Proteins 1994;19(4):302-9.
1994: Schreuder H A; Mattevi A; Obmolova G; Kalk K H; Hol W G; van der Bolt F J; van Berkel W J
Crystal structures of wild-type p-hydroxybenzoate hydroxylase complexed with 4-aminobenzoate,2,4-dihydroxybenzoate, and 2-hydroxy-4-aminobenzoate and of the Tyr222Ala mutant complexed with 2-hydroxy-4-aminobenzoate. Evidence for a proton channel and a new binding mode of the flavin ring.
Biochemistry 1994;33(33):10161-70.
1994: Verlinde C L; Hol W G
Structure-based drug design: progress, results and challenges.
Structure (London, England : 1993) 1994;2(7):577-87.
1994: Mande S C; Mainfroid V; Kalk K H; Goraj K; Martial J A; Hol W G
Crystal structure of recombinant human triosephosphate isomerase at 2.8 A resolution. Triosephosphate isomerase-related human genetic disorders and comparison with the trypanosomal enzyme.
Protein science : a publication of the Protein Society 1994;3(5):810-21.
1994: Merritt E A; Sarfaty S; van den Akker F; L'Hoir C; Martial J A; Hol W G
Crystal structure of cholera toxin B-pentamer bound to receptor GM1 pentasaccharide.
Protein science : a publication of the Protein Society 1994;3(2):166-75.
1994: Merritt E A; Pronk S E; Sixma T K; Kalk K H; van Zanten B A; Hol W G
Structure of partially-activated E. coli heat-labile enterotoxin (LT) at 2.6 A resolution.
FEBS letters 1994;337(1):88-92.
1994: Schreuder H A; de Boer B; Dijkema R; Mulders J; Theunissen H J; Grootenhuis P D; Hol W G
The intact and cleaved human antithrombin III complex as a model for serpin-proteinase interactions.
Nature structural biology 1994;1(1):48-54.
1993: Schreuder H; Arkema A; de Boer B; Kalk K; Dijkema R; Mulders J; Theunissen H; Hol W
Crystallization and preliminary crystallographic analysis of antistasin, a leech-derived inhibitor of blood coagulation factor Xa.
Journal of molecular biology 1993;231(4):1137-8.
1993: Hazes B; Magnus K A; Bonaventura C; Bonaventura J; Dauter Z; Kalk K H; Hol W G
Crystal structure of deoxygenated Limulus polyphemus subunit II hemocyanin at 2.18 A resolution: clues for a mechanism for allosteric regulation.
Protein science : a publication of the Protein Society 1993;2(4):597-619.
1993: Mattevi A; Obmolova G; Kalk K H; van Berkel W J; Hol W G
Three-dimensional structure of lipoamide dehydrogenase from Pseudomonas fluorescens at 2.8 A resolution. Analysis of redox and thermostability properties.
Journal of molecular biology 1993;230(4):1200-15.
1993: Mattevi A; Obmolova G; Kalk K H; Westphal A H; de Kok A; Hol W G
Refined crystal structure of the catalytic domain of dihydrolipoyl transacetylase (E2p) from Azotobacter vinelandii at 2.6 A resolution.
Journal of molecular biology 1993;230(4):1183-99.
1993: Sixma T K; Kalk K H; van Zanten B A; Dauter Z; Kingma J; Witholt B; Hol W G
Refined structure of Escherichia coli heat-labile enterotoxin, a close relative of cholera toxin.
Journal of molecular biology 1993;230(3):890-918.
1993: Mattevi A; Obmolova G; Kalk K H; Teplyakov A; Hol W G
Crystallographic analysis of substrate binding and catalysis in dihydrolipoyl transacetylase (E2p).
Biochemistry 1993;32(15):3887-901.
1993: Rentier-Delrue F; Mande S C; Moyens S; Terpstra P; Mainfroid V; Goraj K; Lion M; Hol W G; Martial J A
Cloning and overexpression of the triosephosphate isomerase genes from psychrophilic and thermophilic bacteria. Structural comparison of the predicted protein sequences.
Journal of molecular biology 1993;229(1):85-93.
1993: Schreuder H; de Boer B; Pronk S; Hol W; Dijkema R; Mulders J; Theunissen H
Crystallization and preliminary X-ray analysis of human antithrombin III.
Journal of molecular biology 1993;229(1):249-50.
1993: Sixma T K; Stein P E; Hol W G; Read R J
Comparison of the B-pentamers of heat-labile enterotoxin and verotoxin-1: two structures with remarkable similarity and dissimilarity.
Biochemistry 1993;32(1):191-8.
1992: Verlinde C L; Witmans C J; Pijning T; Kalk K H; Hol W G; Callens M; Opperdoes F R
Structure of the complex between trypanosomal triosephosphate isomerase and N-hydroxy-4-phosphono-butanamide: binding at the active site despite an "open" flexible loop conformation.
Protein science : a publication of the Protein Society 1992;1(12):1578-84.
1992: Schreuder H A; van der Laan J M; Swarte M B; Kalk K H; Hol W G; Drenth J
Crystal structure of the reduced form of p-hydroxybenzoate hydroxylase refined at 2.3 A resolution.
Proteins 1992;14(2):178-90.
1992: Mattevi A; Obmolova G; Sokatch J R; Betzel C; Hol W G
The refined crystal structure of Pseudomonas putida lipoamide dehydrogenase complexed with NAD+ at 2.45 A resolution.
Proteins 1992;13(4):336-51.
1992: Sixma T K; Aguirre A; Terwisscha van Scheltinga A C; Wartna E S; Kalk K H; Hol W G
Heat-labile enterotoxin crystal forms with variable A/B5 orientation. Analysis of conformational flexibility.
FEBS letters 1992;305(2):81-5.
1992: Verlinde C L; Rudenko G; Hol W G
In search of new lead compounds for trypanosomiasis drug design: a protein structure-based linked-fragment approach.
Journal of computer-aided molecular design 1992;6(2):131-47.
1992: Hazes B; Hol W G
Comparison of the hemocyanin beta-barrel with other Greek key beta-barrels: possible importance of the "beta-zipper" in protein structure and folding.
Proteins 1992;12(3):278-98.
1992: Mattevi A; Obmolova G; Schulze E; Kalk K H; Westphal A H; de Kok A; Hol W G
Atomic structure of the cubic core of the pyruvate dehydrogenase multienzyme complex.
Science (New York, N.Y.) 1992;255(5051):1544-50.
1992: Sixma T K; Pronk S E; Kalk K H; van Zanten B A; Berghuis A M; Hol W G
Lactose binding to heat-labile enterotoxin revealed by X-ray crystallography.
Nature 1992;355(6360):561-4.
1992: Sixma T K; Terwisscha van Scheltinga A C; Kalk K H; Zhou K; Wartna E S; Hol W G
X-ray studies reveal lanthanide binding sites at the A/B5 interface of E. coli heat labile enterotoxin.
FEBS letters 1992;297(1-2):179-82.
1992: Gros P; Teplyakov A V; Hol W G
Effects of eglin-c binding to thermitase: three-dimensional structure comparison of native thermitase and thermitase eglin-c complexes.
Proteins 1992;12(1):63-74.
1991: Schulze E; Westphal A H; Obmolova G; Mattevi A; Hol W G; de Kok A
The catalytic domain of the dihydrolipoyl transacetylase component of the pyruvate dehydrogenase complex from Azotobacter vinelandii and Escherichia coli. Expression, purification, properties and preliminary X-ray analysis.
European journal of biochemistry / FEBS 1991;201(3):561-8.
1991: Noble M E; Verlinde C L; Groendijk H; Kalk K H; Wierenga R K; Hol W G
Crystallographic and molecular modeling studies on trypanosomal triosephosphate isomerase: a critical assessment of the predicted and observed structures of the complex with 2-phosphoglycerate.
Journal of medicinal chemistry 1991;34(9):2709-18.
1991: Wierenga R K; Noble M E; Vriend G; Nauche S; Hol W G
Refined 1.83 A structure of trypanosomal triosephosphate isomerase crystallized in the presence of 2.4 M-ammonium sulphate. A comparison with the structure of the trypanosomal triosephosphate isomerase-glycerol-3-phosphate complex.
Journal of molecular biology 1991;220(4):995-1015.
1991: Mattevi A; Schierbeek A J; Hol W G
Refined crystal structure of lipoamide dehydrogenase from Azotobacter vinelandii at 2.2 A resolution. A comparison with the structure of glutathione reductase.
Journal of molecular biology 1991;220(4):975-94.
1991: Verlinde C L; Noble M E; Kalk K H; Groendijk H; Wierenga R K; Hol W G
Anion binding at the active site of trypanosomal triosephosphate isomerase. Monohydrogen phosphate does not mimic sulphate.
European journal of biochemistry / FEBS 1991;198(1):53-7.
1991: Sixma T K; Pronk S E; Kalk K H; Wartna E S; van Zanten B A; Witholt B; Hol W G
Crystal structure of a cholera toxin-related heat-labile enterotoxin from E. coli.
Nature 1991;351(6325):371-7.
1991: Gros P; Kalk K H; Hol W G
Calcium binding to thermitase. Crystallographic studies of thermitase at 0, 5, and 100 mM calcium.
The Journal of biological chemistry 1991;266(5):2953-61.
1991: Noble M E; Wierenga R K; Lambeir A M; Opperdoes F R; Thunnissen A M; Kalk K H; Groendijk H; Hol W G
The adaptability of the active site of trypanosomal triosephosphate isomerase as observed in the crystal structures of three different complexes.
Proteins 1991;10(1):50-69.
1991: Wierenga R K; Noble M E; Postma J P; Groendijk H; Kalk K H; Hol W G; Opperdoes F R
The crystal structure of the "open" and the "closed" conformation of the flexible loop of trypanosomal triosephosphate isomerase.
Proteins 1991;10(1):33-49.
1991: Magnus K A; Lattman E E; Volbeda A; Hol W G
Hexamers of subunit II from Limulus hemocyanin (a 48-mer) have the same quaternary structure as whole Panulirus hemocyanin molecules.
Proteins 1991;9(4):240-7.
1990: Eschrich K; van Berkel W J; Westphal A H; de Kok A; Mattevi A; Obmolova G; Kalk K H; Hol W G
Engineering of microheterogeneity-resistant p-hydroxybenzoate hydroxylase from Pseudomonas fluorescens.
FEBS letters 1990;277(1-2):197-9.
1990: Vellieux F M; Kalk K H; Drenth J; Hol W G
Structure determination of quinoprotein methylamine dehydrogenase from Thiobacillus versutus.
Acta crystallographica. Section B, Structural science 1990;46 ( Pt 6)():806-23.
1990: Gros P; van Gunsteren W F; Hol W G
Inclusion of thermal motion in crystallographic structures by restrained molecular dynamics.
Science (New York, N.Y.) 1990;249(4973):1149-52.
1990: Schreuder H A; Hol W G; Drenth J
Analysis of the active site of the flavoprotein p-hydroxybenzoate hydroxylase and some ideas with respect to its reaction mechanism.
Biochemistry 1990;29(12):3101-8.
1989: Gros P; Betzel C; Dauter Z; Wilson K S; Hol W G
Molecular dynamics refinement of a thermitase-eglin-c complex at 1.98 A resolution and comparison of two crystal forms that differ in calcium content.
Journal of molecular biology 1989;210(2):347-67.
1989: Gros P; Fujinaga M; Dijkstra B W; Kalk K H; Hol W G
Crystallographic refinement by incorporation of molecular dynamics: thermostable serine protease thermitase complexed with eglin c.
Acta crystallographica. Section B, Structural science 1989;45 ( Pt 5)():488-99.
1989: van der Laan J M; Schreuder H A; Swarte M B; Wierenga R K; Kalk K H; Hol W G; Drenth J
The coenzyme analogue adenosine 5-diphosphoribose displaces FAD in the active site of p-hydroxybenzoate hydroxylase. An x-ray crystallographic investigation.
Biochemistry 1989;28(18):7199-205.
1989: Volbeda A; Hol W G
Crystal structure of hexameric haemocyanin from Panulirus interruptus refined at 3.2 A resolution.
Journal of molecular biology 1989;209(2):249-79.
1989: Vellieux F M; Huitema F; Groendijk H; Kalk K H; Jzn J F; Jongejan J A; Duine J A; Petratos K; Drenth J; Hol W G
Structure of quinoprotein methylamine dehydrogenase at 2.25 A resolution.
The EMBO journal 1989;8(8):2171-8.
1989: Schreuder H A; Prick P A; Wierenga R K; Vriend G; Wilson K S; Hol W G; Drenth J
Crystal structure of the p-hydroxybenzoate hydroxylase-substrate complex refined at 1.9 A resolution. Analysis of the enzyme-substrate and enzyme-product complexes.
Journal of molecular biology 1989;208(4):679-96.
1989: Volbeda A; Feiters M C; Vincent M G; Bouwman E; Dobson B; Kalk K H; Reedijk J; Hol W G
Spectroscopic investigations of Panulirus interruptus hemocyanin in the crystalline state.
European journal of biochemistry / FEBS 1989;181(3):669-73.
1989: Volbeda A; Hol W G
Pseudo 2-fold symmetry in the copper-binding domain of arthropodan haemocyanins. Possible implications for the evolution of oxygen transport proteins.
Journal of molecular biology 1989;206(3):531-46.
1989: Schierbeek A J; Swarte M B; Dijkstra B W; Vriend G; Read R J; Hol W G; Drenth J; Betzel C
X-ray structure of lipoamide dehydrogenase from Azotobacter vinelandii determined by a combination of molecular and isomorphous replacement techniques.
Journal of molecular biology 1989;206(2):365-79.
1989: Van der Laan J M; Swarte M B; Groendijk H; Hol W G; Drenth J
The influence of purification and protein heterogeneity on the crystallization of p-hydroxybenzoate hydroxylase.
European journal of biochemistry / FEBS 1989;179(3):715-24.
1989: Kelders H A; Kalk K H; Hol W G
Crystallization of thermitase, a thermostable subtilisin, from a sodium formate solution by means of an automated procedure.
Journal of molecular biology 1989;205(3):615-6.
1989: Van der Klei I J; Lawson C L; Rozeboom H; Dijkstra B W; Veenhuis M; Harder W; Hol W G
Use of electron microscopy in the examination of lattice defects in crystals of alcohol oxidase.
FEBS letters 1989;244(1):213-6.
1988: Kooystra P J; Kalk K H; Hol W G
Soaking in Cs2SO4 reveals a caesium-aromatic interaction in bovine-liver rhodanese.
European journal of biochemistry / FEBS 1988;177(2):345-9.
1988: Schreuder H A; Hol W G; Drenth J
Molecular modeling reveals the possible importance of a carbonyl oxygen binding pocket for the catalytic mechanism of p-hydroxybenzoate hydroxylase.
The Journal of biological chemistry 1988;263(7):3131-6.
1988: Schreuder H A; van der Laan J M; Hol W G; Drenth J
Crystal structure of p-hydroxybenzoate hydroxylase complexed with its reaction product 3,4-dihydroxybenzoate.
Journal of molecular biology 1988;199(4):637-48.
1988: Volbeda A; Hol W G
Structure of arthropodan hemocyanin.
Progress in clinical and biological research 1988;274():291-307.
1987: Wierenga R K; Kalk K H; Hol W G
Structure determination of the glycosomal triosephosphate isomerase from Trypanosoma brucei brucei at 2.4 A resolution.
Journal of molecular biology 1987;198(1):109-21.
1987: Kelders H A; Kalk K H; Gros P; Hol W G
Automated protein crystallization and a new crystal form of a subtilisin:eglin complex.
Protein engineering 1987;1(4):301-3.
1987: Read R J; Wierenga R K; Groendijk H; Hol W G; Lambeir A; Opperdoes F R
Preliminary crystallographic studies of glycosomal glyceraldehyde phosphate dehydrogenase from Trypanosoma brucei brucei.
Journal of molecular biology 1987;194(3):573-5.
1986: Vellieux F M; Frank J; Swarte M B; Groendijk H; Duine J A; Drenth J; Hol W G
Purification, crystallization and preliminary X-ray investigation of quinoprotein methylamine dehydrogenase from Thiobacillus versutus.
European journal of biochemistry / FEBS 1986;154(2):383-6.
1985: Pronk S E; Hofstra H; Groendijk H; Kingma J; Swarte M B; Dorner F; Drenth J; Hol W G; Witholt B
Heat-labile enterotoxin of Escherichia coli. Characterization of different crystal forms.
The Journal of biological chemistry 1985;260(25):13580-4.

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