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Wim Hol

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X Ray Crystallography

Chemicals & Drugs

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Escherichia coli

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TOP 3
Konstantin Korotkov; Wim G J Hol
Crystal structure of the pilotin from the enterohemorrhagic Escherichia coli type II secretion system.
Patricia M Legler; Dan Zabetakis; George P Anderson; Wim G J Hol; Anita Lam; Ellen R Goldman
Structure of a low-melting-temperature anti-cholera toxin: llama V(H)H domain.
Young-Jun Park; Wim G J Hol
Explorations of linked editosome domains leading to the discovery of motifs defining conserved pockets in editosome OB-folds.

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All Publications

2013: Konstantin Korotkov; Wim G J Hol
Crystal structure of the pilotin from the enterohemorrhagic Escherichia coli type II secretion system.
Journal of structural biology 2013;182(2):186-91.
2013: Patricia M Legler; Dan Zabetakis; George P Anderson; Wim G J Hol; Anita Lam; Ellen R Goldman
Structure of a low-melting-temperature anti-cholera toxin: llama V(H)H domain.
Acta crystallographica. Section F, Structural biology and crystallization communications 2013;69(Pt 2):90-3.
2012: Young-Jun Park; Wim G J Hol
Explorations of linked editosome domains leading to the discovery of motifs defining conserved pockets in editosome OB-folds.
Journal of structural biology 2012;180(2):362-73.
2012: Cho Yeow Koh; Jiyun Liu; Ranae M Ranade; Sayaka Shibata; Christophe L M J Verlinde; Mingyan Yu; Frederick S Buckner; Erkang Fan; John R Gillespie; Jessica E Kim; Wim G J Hol
Distinct states of methionyl-tRNA synthetase indicate inhibitor binding by conformational selection.
Structure (London, England : 1993) 2012;20(10):1681-91.
2012: Eric T Larson; Frederick S Buckner; Erkang Fan; Wim G J Hol; Jessica E Kim; Alberto J Napuli; Wesley C Van Voorhis; Christophe L M J Verlinde; Frank H Zucker; Ethan A Merritt
Structure of the prolyl-tRNA synthetase from the eukaryotic pathogen Giardia lamblia.
Acta crystallographica. Section D, Biological crystallography 2012;68(Pt 9):1194-200.
2012: Young-Jun Park; Jan Steyaert; Meiting Wu; Tanya Budiarto; Els Pardon; Wim G J Hol
The structure of the C-terminal domain of the largest editosome interaction protein and its role in promoting RNA binding by RNA-editing ligase L2.
Nucleic acids research 2012;40(14):6966-77.
2012: Sayaka Shibata; Christophe L M J Verlinde; Frank H Zucker; Frederick S Buckner; J Robert Gillespie; Wim G J Hol; Jessica E Kim; Cho Yeow Koh; Joy U Laydbak; Ranae M Ranade; Erkang Fan
Urea-based inhibitors of Trypanosoma brucei methionyl-tRNA synthetase: selectivity and in vivo characterization.
Journal of medicinal chemistry 2012;55(14):6342-51.
2012: Konstantin V Korotkov; maria sandkvist; Wim G J Hol
The type II secretion system: biogenesis, molecular architecture and mechanism.
Nature reviews. Microbiology 2012;10(5):336-51.
2012: Jürgen Bosch; Matthew H Paige; Akhil B Vaidya; Lawrence W Bergman; Wim G J Hol
Crystal structure of GAP50, the anchor of the invasion machinery in the inner membrane complex of Plasmodium falciparum.
Journal of structural biology 2012;178(1):61-73.
2012: Eric T Larson; David J Leibly; Dustin J Maly; Ryan C Murphy; Kayode K Ojo; B Gayani K Perera; Molly C Reid; Wesley C Van Voorhis; Christophe L M J Verlinde; Zhongsheng Zhang; Edward J Dale; Erkang Fan; Anna M W Fox; Stephen N Hewitt; Wim G J Hol; Steven M Johnson; Jae Kim; Jessica E Kim; Ethan A Merritt
Multiple determinants for selective inhibition of apicomplexan calcium-dependent protein kinase CDPK1.
Journal of medicinal chemistry 2012;55(6):2803-10.
2012: Young-Jun Park; Jan Steyaert; Meiting Wu; Els Pardon; Wim G J Hol
Crystal structure of a heterodimer of editosome interaction proteins in complex with two copies of a cross-reacting nanobody.
Nucleic acids research 2012;40(4):1828-40.
2011: Konstantin Korotkov; Els Pardon; Jonathan Pruneda; maria sandkvist; Jan Steyaert; Stewart Turley; Annie Héroux; Randall K Holmes; Michael G Jobling; Tayna Johnson; Wim G J Hol
Structural and functional studies on the interaction of GspC and GspD in the type II secretion system.
PLoS pathogens 2011;7(9):e1002228.
2011: Sayaka Shibata; Wesley C Van Voorhis; Christophe L M J Verlinde; Zhongsheng Zhang; Frank H Zucker; Wim G J Hol; Angela M Kelley; Konstantin Korotkov; Ethan A Merritt; Natasha Mueller; Alberto Napuli; Jennifer Ross; Frederick S Buckner; Jaclyn Delarosa; Erkang Fan
Screening a fragment cocktail library using ultrafiltration.
Analytical and bioanalytical chemistry 2011;401(5):1585-91.
2011: Konstantin Korotkov; Tamir Gonen; Wim G J Hol
Secretins: dynamic channels for protein transport across membranes.
Trends in biochemical sciences 2011;36(8):433-43.
2011: Eric T Larson; Jessica E Kim; Lisa J Castaneda; Alberto J Napuli; Zhongsheng Zhang; Erkang Fan; Frank H Zucker; Christophe L M J Verlinde; Frederick S Buckner; Wesley C Van Voorhis; Wim G J Hol; Ethan A Merritt
The double-length tyrosyl-tRNA synthetase from the eukaryote Leishmania major forms an intrinsically asymmetric pseudo-dimer.
Journal of molecular biology 2011;409(2):159-76.
2011: Steve L Reichow; Ji Sun; Jaclyn R Delarosa; Melissa Gonen; Wim G J Hol; Konstantin Korotkov; Tamir Gonen
The binding of cholera toxin to the periplasmic vestibule of the type II secretion channel.
Channels (Austin, Tex.) 2011;5(3):215-8.
2011: Sayaka Shibata; John R Gillespie; Angela M Kelley; Alberto J Napuli; Zhongsheng Zhang; Kuzma V Kovzun; Ranae M Pefley; Jocelyn Lam; Frank H Zucker; Wesley C Van Voorhis; Ethan A Merritt; Wim G J Hol; Christophe L M J Verlinde; Erkang Fan; Frederick S Buckner
Selective inhibitors of methionyl-tRNA synthetase have potent activity against Trypanosoma brucei Infection in Mice.
Antimicrobial agents and chemotherapy 2011;55(5):1982-9.
2011: Ethan A Merritt; Tracy L Arakaki; Robert Gillespie; Alberto J Napuli; Jessica E Kim; Frederick S Buckner; Wesley C Van Voorhis; Christophe L M J Verlinde; Erkang Fan; Frank Zucker; Wim G J Hol
Crystal structures of three protozoan homologs of tryptophanyl-tRNA synthetase.
Molecular and biochemical parasitology 2011;177(1):20-8.
2011: Kayode K Ojo; Tracy L Arakaki; Alberto J Napuli; Krishna K Inampudi; Katelyn R Keyloun; Li Zhang; Wim G J Hol; Christophe L M J Verlinde; Ethan A Merritt; Wesley C Van Voorhis
Structure determination of glycogen synthase kinase-3 from Leishmania major and comparative inhibitor structure-activity relationships with Trypanosoma brucei GSK-3.
Molecular and biochemical parasitology 2011;176(2):98-108.
2011: Meiting Wu; Young-Jun Park; Els Pardon; Stewart Turley; Andrew Hayhurst; Junpeng Deng; Jan Steyaert; Wim G J Hol
Structures of a key interaction protein from the Trypanosoma brucei editosome in complex with single domain antibodies.
Journal of structural biology 2011;174(1):124-36.
2011: Marcella Patrick; Konstantin V Korotkov; Wim G J Hol; maria sandkvist
Oligomerization of EpsE coordinates residues from multiple subunits to facilitate ATPase activity.
The Journal of biological chemistry 2011;286(12):10378-86.
2011: Eric T Larson; Jessica E Kim; Frank H Zucker; Angela Kelley; Natascha Mueller; Alberto J Napuli; Christophe L M J Verlinde; Erkang Fan; Frederick S Buckner; Wesley C Van Voorhis; Ethan A Merritt; Wim G J Hol
Structure of Leishmania major methionyl-tRNA synthetase in complex with intermediate products methionyladenylate and pyrophosphate.
Biochimie 2011;93(3):570-82.
2011: Matthew D Gray; Michael Bagdasarian; Wim G J Hol; maria sandkvist
In vivo cross-linking of EpsG to EpsL suggests a role for EpsL as an ATPase-pseudopilin coupling protein in the Type II secretion system of Vibrio cholerae.
Molecular microbiology 2011;79(3):786-98.
2010: Steve L Reichow; Konstantin V Korotkov; Wim G J Hol; Tamir Gonen
Structure of the cholera toxin secretion channel in its closed state.
Nature structural & molecular biology 2010;17(10):1226-32.
2010: Tracy L Arakaki; Megan Carter; Alberto J Napuli; Christophe L M J Verlinde; Erkang Fan; Frank Zucker; Frederick S Buckner; Wesley C Van Voorhis; Wim G J Hol; Ethan A Merritt
The structure of tryptophanyl-tRNA synthetase from Giardia lamblia reveals divergence from eukaryotic homologs.
Journal of structural biology 2010;171(2):238-43.
2010: Frank H Zucker; Jaclyn dela Rosa; Tracy L Arakaki; Frederick S Buckner; Lisa J Castaneda; Erkang Fan; Jessica Kim; Eric T Larson; Ethan A Merritt; Natascha Mueller; Stephen R Nakazawa Hewitt; Alberto J Napuli; Jenni Ross; Christine Stewart; Easwara Subramanian; Wesley C Van Voorhis; Christophe L M J Verlinde; Liren Xiao; Li Zhang; Wim G J Hol
Prediction of protein crystallization outcome using a hybrid method.
Journal of structural biology 2010;171(1):64-73.
2010: Kayode K Ojo; Eric T Larson; Katelyn R Keyloun; Lisa J Castaneda; Amy E Derocher; Krishna K Inampudi; Jessica E Kim; Tracy L Arakaki; Ryan C Murphy; Li Zhang; Alberto J Napuli; Dustin J Maly; Christophe Verlinde; Frederick S Buckner; Marilyn Parsons; Wim G J Hol; Ethan A Merritt; Wesley C Van Voorhis
Toxoplasma gondii calcium-dependent protein kinase 1 is a target for selective kinase inhibitors.
Nature structural & molecular biology 2010;17(5):602-7.
2010: Gregory J Crowther; Panqing He; Philip P Rodenbough; Andrew P Thomas; Kuzma V Kovzun; David J Leibly; Janhavi Bhandari; Lisa J Castaneda; Wim G J Hol; Michael H Gelb; Alberto J Napuli; Wesley C Van Voorhis
Use of thermal melt curves to assess the quality of enzyme preparations.
Analytical biochemistry 2010;399(2):268-75.
2010: Ethan A Merritt; Tracy L Arakaki; John R Gillespie; Eric T Larson; Angela Kelley; Natascha Mueller; Alberto J Napuli; Jessica Kim; Li Zhang; Christophe Verlinde; Erkang Fan; Frank Zucker; Frederick S Buckner; Wesley C Van Voorhis; Wim G J Hol
Crystal structures of trypanosomal histidyl-tRNA synthetase illuminate differences between eukaryotic and prokaryotic homologs.
Journal of molecular biology 2010;397(2):481-94.
2010: Eric T Larson; Devaraja G Mudeppa; John R Gillespie; Natascha Mueller; Alberto J Napuli; Jennifer A Arif; Jenni Ross; Tracy L Arakaki; Angela Lauricella; George deTitta; Joseph Luft; Frank Zucker; Christophe Verlinde; Erkang Fan; Wesley C Van Voorhis; Frederick S Buckner; Pradipsinh K Rathod; Wim G J Hol; Ethan A Merritt
The crystal structure and activity of a putative trypanosomal nucleoside phosphorylase reveal it to be a homodimeric uridine phosphorylase.
Journal of molecular biology 2010;396(5):1244-59.
2010: Achim Schnaufer; Meiting Wu; Young-jun Park; Tadashi Nakai; Junpeng Deng; Rose Proff; Wim G J Hol; Kenneth D Stuart
A protein-protein interaction map of trypanosome ~20S editosomes.
The Journal of biological chemistry 2010;285(8):5282-95.
2010: Ethan A Merritt; Tracy L Arakaki; Eric T Larson; Angela Kelley; Natascha Mueller; Alberto J Napuli; Li Zhang; George Deditta; Joseph Luft; Christophe Verlinde; Erkang Fan; Frank Zucker; Frederick S Buckner; Wesley C Van Voorhis; Wim G J Hol
Crystal structure of the aspartyl-tRNA synthetase from Entamoeba histolytica.
Molecular and biochemical parasitology 2010;169(2):95-100.
2009: Jan Abendroth; Allison C Kreger; Wim G J Hol
The dimer formed by the periplasmic domain of EpsL from the Type 2 Secretion System of Vibrio parahaemolyticus.
Journal of structural biology 2009;168(2):313-22.
2009: Wesley C Van Voorhis; Wim G J Hol; Peter J Myler; Lance J Stewart
The role of medical structural genomics in discovering new drugs for infectious diseases.
PLoS computational biology 2009;5(10):e1000530.
2009: Konstantin V Korotkov; Miranda D Gray; Allison Kreger; Stewart Turley; maria sandkvist; Wim G J Hol
Calcium is essential for the major pseudopilin in the type 2 secretion system.
The Journal of biological chemistry 2009;284(38):25466-70.
2009: Gregory J Crowther; Alberto J Napuli; Andrew P Thomas; Diana J Chung; Kuzma V Kovzun; David J Leibly; Lisa J Castaneda; Janhavi Bhandari; Christopher J Damman; Raymond Hui; Wim G J Hol; Frederick S Buckner; Christophe Verlinde; Zhongsheng Zhang; Erkang Fan; Wesley C Van Voorhis
Buffer optimization of thermal melt assays of Plasmodium proteins for detection of small-molecule ligands.
Journal of biomolecular screening 2009;14(6):700-7.
2009: Jan Abendroth; Daniel D Mitchell; Konstantin Korotkov; Tayna Johnson; Allison Kreger; maria sandkvist; Wim G J Hol
The three-dimensional structure of the cytoplasmic domains of EpsF from the type 2 secretion system of Vibrio cholerae.
Journal of structural biology 2009;166(3):303-15.
2009: Anita Y Lam; Els Pardon; Konstantin Korotkov; Wim G J Hol; Jan Steyaert
Nanobody-aided structure determination of the EpsI:EpsJ pseudopilin heterodimer from Vibrio vulnificus.
Journal of structural biology 2009;166(1):8-15.
2009: Konstantin Korotkov; Els Pardon; Jan Steyaert; Wim G J Hol
Crystal structure of the N-terminal domain of the secretin GspD from ETEC determined with the assistance of a nanobody.
Structure (London, England : 1993) 2009;17(2):255-65.
2009: Christophe L M J Verlinde; Erkang Fan; Sayaka Shibata; Zongsheng Zhang; Zhihua Sun; Wei Deng; Jennifer Ross; Jessica Kim; Liren Xiao; Tracy L Arakaki; Jürgen Bosch; Jonathan M Caruthers; Eric T Larson; Isolde Letrong; Alberto Napuli; Angela Kelly; Natasha Mueller; Frank Zucker; Wesley C Van Voorhis; Frederick S Buckner; Ethan A Merritt; Wim G J Hol
Fragment-based cocktail crystallography by the medical structural genomics of pathogenic protozoa consortium.
Current topics in medicinal chemistry 2009;9(18):1678-87.
2008: Eric T Larson; Wei Deng; Brian Krumm; Alberto Napuli; Natascha Mueller; Wesley C Van Voorhis; Frederick S Buckner; Erkang Fan; Angela Lauricella; George deTitta; Joseph Luft; Frank Zucker; Wim G J Hol; Christophe Verlinde; Ethan A Merritt
Structures of substrate- and inhibitor-bound adenosine deaminase from a human malaria parasite show a dramatic conformational change and shed light on drug selectivity.
Journal of molecular biology 2008;381(4):975-88.
2008: Parthasrathy Sampathkumar; Claudia Roach; Paul A M Michels; Wim G J Hol
Structural insights into the recognition of peroxisomal targeting signal 1 by Trypanosoma brucei peroxin 5.
Journal of molecular biology 2008;381(4):867-80.
2008: Ethan A Merritt; Margaret Holmes; Frederick S Buckner; Wesley C Van Voorhis; Erin Quartly; Eric M Phizicky; Angela Lauricella; Joseph Luft; George deTitta; Helen Neely; Frank Zucker; Wim G J Hol
Structure of a Trypanosoma brucei alpha/beta-hydrolase fold protein with unknown function.
Acta crystallographica. Section F, Structural biology and crystallization communications 2008;64(Pt 6):474-8.
2008: Konstantin Korotkov; Wim G J Hol
Structure of the GspK-GspI-GspJ complex from the enterotoxigenic Escherichia coli type 2 secretion system.
Nature structural & molecular biology 2008;15(5):462-8.
2008: Goragot Wisedchaisri; Meiting Wu; emily glenn; Wim G J Hol
Crystal structures of the response regulator DosR from Mycobacterium tuberculosis suggest a helix rearrangement mechanism for phosphorylation activation.
Journal of molecular biology 2008;378(1):227-42.
2008: Tracy L Arakaki; Frederick S Buckner; John R Gillespie; Nicholas A Malmquist; Margaret A Phillips; Oleksandr Kalyuzhniy; Joseph Luft; George deTitta; Christophe Verlinde; Wesley C Van Voorhis; Wim G J Hol; Ethan A Merritt
Characterization of Trypanosoma brucei dihydroorotate dehydrogenase as a possible drug target; structural, kinetic and RNAi studies.
Molecular microbiology 2008;68(1):37-50.
2008: Marissa E Yanez; Konstantin Korotkov; Jan Abendroth; Wim G J Hol
Structure of the minor pseudopilin EpsH from the Type 2 secretion system of Vibrio cholerae.
Journal of molecular biology 2008;377(1):91-103.
2008: Marissa E Yanez; Konstantin Korotkov; Jan Abendroth; Wim G J Hol
The crystal structure of a binary complex of two pseudopilins: EpsI and EpsJ from the type 2 secretion system of Vibrio vulnificus.
Journal of molecular biology 2008;375(2):471-86.
2007: Jürgen Bosch; Stewart Turley; Claudia Roach; Thomas M Daly; Lawrence W Bergman; Wim G J Hol
The closed MTIP-myosin A-tail complex from the malaria parasite invasion machinery.
Journal of molecular biology 2007;372(1):77-88.
2007: Jürgen Bosch; Carlos A Buscaglia; Brian Krumm; Bjarni P Ingason; Robert Lucas; Claudia Roach; Timothy Cardozo; Victor Nussenzweig; Wim G J Hol
Aldolase provides an unusual binding site for thrombospondin-related anonymous protein in the invasion machinery of the malaria parasite.
Proceedings of the National Academy of Sciences of the United States of America 2007;104(17):7015-20.
2007: Tracy Arakaki; Helen Neely; Erica Boni; Natasha Mueller; Frederick S Buckner; Wesley C Van Voorhis; Angela Lauricella; George deTitta; Joseph Luft; Wim G J Hol; Ethan A Merritt
The structure of Plasmodium vivax phosphatidylethanolamine-binding protein suggests a functional motif containing a left-handed helix.
Acta crystallographica. Section F, Structural biology and crystallization communications 2007;63(Pt 3):178-82.
2007: Carlos A Buscaglia; Wim G J Hol; Victor Nussenzweig; Timothy Cardozo
Modeling the interaction between aldolase and the thrombospondin-related anonymous protein, a key connection of the malaria parasite invasion machinery.
Proteins 2007;66(3):528-37.
2007: Eaton E Lattman; Wim G J Hol; Eddy Arnold
Drug design for the third world: a conference report.
Proteins 2007;66(3):509-12.
2007: Goragot Wisedchaisri; C James Chou; Meiting Wu; Claudia Roach; Adrian E Rice; Randall K Holmes; Craig Beeson; Wim G J Hol
Crystal structures, metal activation, and DNA-binding properties of two-domain IdeR from Mycobacterium tuberculosis.
Biochemistry 2007;46(2):436-47.
2007: Jodi L Camberg; Tayna Johnson; Marcella Patrick; Jan Abendroth; Wim G J Hol; maria sandkvist
Synergistic stimulation of EpsE ATP hydrolysis by EpsL and acidic phospholipids.
The EMBO journal 2007;26(1):19-27.
2006: Konstantin Korotkov; Brian Krumm; Michael Bagdasarian; Wim G J Hol
Structural and functional studies of EpsC, a crucial component of the type 2 secretion system from Vibrio cholerae.
Journal of molecular biology 2006;363(2):311-21.
2006: Jürgen Bosch; Mark A Robien; Christopher Mehlin; Erica Boni; Aaron Riechers; Frederick S Buckner; Wesley C Van Voorhis; Peter J Myler; Elizabeth A Worthey; George deTitta; Joseph Luft; Angela Lauricella; Stacey Gulde; Lori A Anderson; Oleksandr Kalyuzhniy; Helen M Neely; Jenni Ross; Thomas N Earnest; Michael Soltis; Lori Schoenfeld; Frank Zucker; Ethan A Merritt; Erkang Fan; Christophe Verlinde; Wim G J Hol
Using fragment cocktail crystallography to assist inhibitor design of Trypanosoma brucei nucleoside 2-deoxyribosyltransferase.
Journal of medicinal chemistry 2006;49(20):5939-46.
2006: Christopher Mehlin; Erica Boni; Frederick S Buckner; Linnea Engel; Tiffany Feist; Michael H Gelb; Lutfiyah Haji; David Kim; Colleen Liu; Natascha Mueller; Peter J Myler; J T Reddy; Joshua N Sampson; E Subramanian; Wesley C Van Voorhis; Elizabeth A Worthey; Frank Zucker; Wim G J Hol
Heterologous expression of proteins from Plasmodium falciparum: results from 1000 genes.
Molecular and biochemical parasitology 2006;148(2):144-60.
2006: Parthasrathy Sampathkumar; Stewart Turley; Carol Hopkins Sibley; Wim G J Hol
NADP+ expels both the co-factor and a substrate analog from the Mycobacterium tuberculosis ThyX active site: opportunities for anti-bacterial drug design.
Journal of molecular biology 2006;360(1):1-6.
2006: Margaret A Holmes; Frederick S Buckner; Wesley C Van Voorhis; Christophe Verlinde; Christopher Mehlin; Erica Boni; George deTitta; Joseph Luft; Angela Lauricella; Lori Anderson; Oleksandr Kalyuzhniy; Frank Zucker; Lori W Schoenfeld; Thomas N Earnest; Wim G J Hol; Ethan A Merritt
Structure of ribose 5-phosphate isomerase from Plasmodium falciparum.
Acta crystallographica. Section F, Structural biology and crystallization communications 2006;62(Pt 5):427-31.
2006: Douglas R Davies; Adeel Mushtaq; Heidrun Interthal; James J Champoux; Wim G J Hol
The structure of the transition state of the heterodimeric topoisomerase I of Leishmania donovani as a vanadate complex with nicked DNA.
Journal of molecular biology 2006;357(4):1202-10.
2006: Jürgen Bosch; Stewart Turley; Thomas M Daly; Stephen M Bogh; Michelle L Villasmil; Claudia Roach; Na Zhou; Joanne M Morrisey; Akhil B Vaidya; Lawrence W Bergman; Wim G J Hol
Structure of the MTIP-MyoA complex, a key component of the malaria parasite invasion motor.
Proceedings of the National Academy of Sciences of the United States of America 2006;103(13):4852-7.
2006: Mark A Robien; Jürgen Bosch; Frederick S Buckner; Wesley C Van Voorhis; Elizabeth A Worthey; Peter J Myler; Christopher Mehlin; Erica Boni; Oleksandr Kalyuzhniy; Lori Anderson; Angela Lauricella; Stacey Gulde; Joseph Luft; George deTitta; Jonathan M Caruthers; Keith O Hodgson; Michael Soltis; Frank Zucker; Christophe Verlinde; Ethan A Merritt; Lori W Schoenfeld; Wim G J Hol
Crystal structure of glyceraldehyde-3-phosphate dehydrogenase from Plasmodium falciparum at 2.25 A resolution reveals intriguing extra electron density in the active site.
Proteins 2006;62(3):570-7.
2006: Margaret A Holmes; Frederick S Buckner; Wesley C Van Voorhis; Christopher Mehlin; Erica Boni; Thomas N Earnest; George deTitta; Joseph Luft; Angela Lauricella; Lori Anderson; Oleksandr Kalyuzhniy; Frank Zucker; Lori W Schoenfeld; Wim G J Hol; Ethan A Merritt
Structure of the conserved hypothetical protein MAL13P1.257 from Plasmodium falciparum.
Acta crystallographica. Section F, Structural biology and crystallization communications 2006;62(Pt 3):180-5.
2006: Tracy Arakaki; Isolde Le Trong; Eric M Phizicky; Erin Quartley; George deTitta; Joseph Luft; Angela Lauricella; Lori Anderson; Oleksandr Kalyuzhniy; Elizabeth A Worthey; Peter J Myler; David Kim; David Baker; Wim G J Hol; Ethan A Merritt
Structure of Lmaj006129AAA, a hypothetical protein from Leishmania major.
Acta crystallographica. Section F, Structural biology and crystallization communications 2006;62(Pt 3):175-9.
2006: Tayna Johnson; Jan Abendroth; Wim G J Hol; maria sandkvist
Type II secretion: from structure to function.
FEMS microbiology letters 2006;255(2):175-86.
2006: Jonathan M Caruthers; Jürgen Bosch; Frederick S Buckner; Wesley C Van Voorhis; Peter J Myler; Elizabeth A Worthey; Christopher Mehlin; Erica Boni; George deTitta; Joseph Luft; Angela Lauricella; Oleksandr Kalyuzhniy; Lori Anderson; Frank Zucker; Michael Soltis; Wim G J Hol
Structure of a ribulose 5-phosphate 3-epimerase from Plasmodium falciparum.
Proteins 2006;62(2):338-42.
2005: Junpeng Deng; Nancy Lewis Ernst; Stewart Turley; Kenneth D Stuart; Wim G J Hol
Structural basis for UTP specificity of RNA editing TUTases from Trypanosoma brucei.
The EMBO journal 2005;24(23):4007-17.
2005: Goragot Wisedchaisri; Meiting Wu; Adrian E Rice; David M Roberts; emily glenn; Wim G J Hol
Structures of Mycobacterium tuberculosis DosR and DosR-DNA complex involved in gene activation during adaptation to hypoxic latency.
Journal of molecular biology 2005;354(3):630-41.
2005: Jonathan M Caruthers; Frank Zucker; Elizabeth A Worthey; Peter J Myler; Frederick S Buckner; Wes Van Voorhuis; Christopher Mehlin; Erica Boni; Tiffany Feist; Joseph Luft; Stacey Gulde; Angela Lauricella; Oleksandr Kaluzhniy; Lori Anderson; Isolde Le Trong; Margaret A Holmes; Thomas N Earnest; Michael Soltis; Keith O Hodgson; Wim G J Hol; Ethan A Merritt
Crystal structures and proposed structural/functional classification of three protozoan proteins from the isochorismatase superfamily.
Protein science : a publication of the Protein Society 2005;14(11):2887-94.
2005: Parthasrathy Sampathkumar; Stewart Turley; Jonathan E Ulmer; Ho Gun Rhie; Carol Hopkins Sibley; Wim G J Hol
Structure of the Mycobacterium tuberculosis flavin dependent thymidylate synthase (MtbThyX) at 2.0A resolution.
Journal of molecular biology 2005;352(5):1091-104.
2005: Claudia Schnick; Mark A Robien; Andrzej M Brzozowski; Eleanor J Dodson; Garib N Murshudov; Lori Anderson; Joseph Luft; Christopher Mehlin; Wim G J Hol; James A Brannigan; Anthony J Wilkinson
Structures of Plasmodium falciparum purine nucleoside phosphorylase complexed with sulfate and its natural substrate inosine.
Acta crystallographica. Section D, Biological crystallography 2005;61(Pt 9):1245-54.
2005: Claire J O'Neal; Michael G Jobling; Randall K Holmes; Wim G J Hol
Structural basis for the activation of cholera toxin by human ARF6-GTP.
Science (New York, N.Y.) 2005;309(5737):1093-6.
2005: Jan Abendroth; Paul Murphy; maria sandkvist; Michael Bagdasarian; Wim G J Hol
The X-ray structure of the type II secretion system complex formed by the N-terminal domain of EpsE and the cytoplasmic domain of EpsL of Vibrio cholerae.
Journal of molecular biology 2005;348(4):845-55.
2005: Jiyun Liu; Zhongsheng Zhang; Xiaojian Tan; Wim G J Hol; Christophe Verlinde; Erkang Fan
Protein heterodimerization through ligand-bridged multivalent pre-organization: enhancing ligand binding toward both protein targets.
Journal of the American Chemical Society 2005;127(7):2044-5.
2004: C James Chou; Goragot Wisedchaisri; Ryan R Monfeli; Diana Oram; Randall K Holmes; Wim G J Hol; Craig Beeson
Functional studies of the Mycobacterium tuberculosis iron-dependent regulator.
The Journal of biological chemistry 2004;279(51):53554-61.
2004: Jan Abendroth; Michael Bagdasarian; maria sandkvist; Wim G J Hol
The structure of the cytoplasmic domain of EpsL, an inner membrane component of the type II secretion system of Vibrio cholerae: an unusual member of the actin-like ATPase superfamily.
Journal of molecular biology 2004;344(3):619-33.
2004: Douglas R Davies; Wim G J Hol
The power of vanadate in crystallographic investigations of phosphoryl transfer enzymes.
FEBS letters 2004;577(3):315-21.
2004: Juliette Moyersoen; Jungwoo Choe; Erkang Fan; Wim G J Hol; Paul A M Michels
Biogenesis of peroxisomes and glycosomes: trypanosomatid glycosome assembly is a promising new drug target.
FEMS microbiology reviews 2004;28(5):603-43.
2004: Zhongsheng Zhang; Jiyun Liu; Christophe Verlinde; Wim G J Hol; Erkang Fan
Large cyclic peptides as cores of multivalent ligands: application to inhibitors of receptor binding by cholera toxin.
The Journal of organic chemistry 2004;69(22):7737-40.
2004: Junpeng Deng; Achim Schnaufer; Reza Salavati; Kenneth D Stuart; Wim G J Hol
High resolution crystal structure of a key editosome enzyme from Trypanosoma brucei: RNA editing ligase 1.
Journal of molecular biology 2004;343(3):601-13.
2004: Erkang Fan; Claire J O'Neal; Daniel D Mitchell; Mark A Robien; Zhongsheng Zhang; Jason Pickens; Xiao-Jian Tan; Konstantin Korotkov; Claudia Roach; Brian Krumm; Christophe Verlinde; Ethan A Merritt; Wim G J Hol
Structural biology and structure-based inhibitor design of cholera toxin and heat-labile enterotoxin.
International journal of medical microbiology : IJMM 2004;294(4):217-23.
2004: Goragot Wisedchaisri; Randall K Holmes; Wim G J Hol
Crystal structure of an IdeR-DNA complex reveals a conformational change in activated IdeR for base-specific interactions.
Journal of molecular biology 2004;342(4):1155-69.
2004: Jason Pickens; Daniel D Mitchell; Jiyun Liu; Xiaojian Tan; Zhongsheng Zhang; Christophe Verlinde; Wim G J Hol; Erkang Fan
Nonspanning bivalent ligands as improved surface receptor binding inhibitors of the cholera toxin B pentamer.
Chemistry & biology 2004;11(9):1205-15.
2004: M S R Sastry; Paulene M Quigley; Wim G J Hol; François Baneyx
The linker-loop region of Escherichia coli chaperone Hsp31 functions as a gate that modulates high-affinity substrate binding at elevated temperatures.
Proceedings of the National Academy of Sciences of the United States of America 2004;101(23):8587-92.
2004: David M Roberts; Reiling P Liao; Goragot Wisedchaisri; Wim G J Hol; emily glenn
Two sensor kinases contribute to the hypoxic response of Mycobacterium tuberculosis.
The Journal of biological chemistry 2004;279(22):23082-7.
2004: Jan Abendroth; Adrian E Rice; Karen McLuskey; Michael Bagdasarian; Wim G J Hol
The crystal structure of the periplasmic domain of the type II secretion system protein EpsM from Vibrio cholerae: the simplest version of the ferredoxin fold.
Journal of molecular biology 2004;338(3):585-96.
2004: Zhongsheng Zhang; Jason Pickens; Wim G J Hol; Erkang Fan
Solution- and solid-phase syntheses of guanidine-bridged, water-soluble linkers for multivalent ligand design.
Organic letters 2004;6(9):1377-80.
2004: Claire J O'Neal; Edward I Amaya; Michael G Jobling; Randall K Holmes; Wim G J Hol
Crystal structures of an intrinsically active cholera toxin mutant yield insight into the toxin activation mechanism.
Biochemistry 2004;43(13):3772-82.
2004: Mark A Robien; Kiet T Nguyen; Abhinav Kumar; Irwin Hirsh; Stewart Turley; Dehua Pei; Wim G J Hol
An improved crystal form of Plasmodium falciparum peptide deformylase.
Protein science : a publication of the Protein Society 2004;13(4):1155-63.
2004: Daniel D Mitchell; Jason Pickens; Konstantin Korotkov; Erkang Fan; Wim G J Hol
3,5-Substituted phenyl galactosides as leads in designing effective cholera toxin antagonists; synthesis and crystallographic studies.
Bioorganic & medicinal chemistry 2004;12(5):907-20.
2004: Douglas R Davies; Heidrun Interthal; James J Champoux; Wim G J Hol
Explorations of peptide and oligonucleotide binding sites of tyrosyl-DNA phosphodiesterase using vanadate complexes.
Journal of medicinal chemistry 2004;47(4):829-37.
2004: Heidrun Interthal; Paulene M Quigley; Wim G J Hol; James J Champoux
The role of lysine 532 in the catalytic mechanism of human topoisomerase I.
The Journal of biological chemistry 2004;279(4):2984-92.
2004: Joanne I Yeh; Véronique Charrier; Joao Paulo; Lihui Hou; Emmanuelle Darbon; Al Claiborne; Wim G J Hol; Josef Deutscher
Structures of enterococcal glycerol kinase in the absence and presence of glycerol: correlation of conformation to substrate binding and a mechanism of activation by phosphorylation.
Biochemistry 2004;43(2):362-73.
2004: Paulene M Quigley; Konstantin Korotkov; François Baneyx; Wim G J Hol
A new native EcHsp31 structure suggests a key role of structural flexibility for chaperone function.
Protein science : a publication of the Protein Society 2004;13(1):269-77.
2004: Junpeng Deng; Douglas R Davies; Goragot Wisedchaisri; Meiting Wu; Wim G J Hol; Christopher Mehlin
An improved protocol for rapid freezing of protein samples for long-term storage.
Acta crystallographica. Section D, Biological crystallography 2004;60(Pt 1):203-4.
2003: Carlos A Buscaglia; Isabelle Coppens; Wim G J Hol; Victor Nussenzweig
Sites of interaction between aldolase and thrombospondin-related anonymous protein in plasmodium.
Molecular biology of the cell 2003;14(12):4947-57.
2003: Mark A Robien; Brian Krumm; maria sandkvist; Wim G J Hol
Crystal structure of the extracellular protein secretion NTPase EpsE of Vibrio cholerae.
Journal of molecular biology 2003;333(3):657-74.
2003: Jungwoo Choe; Juliette Moyersoen; Claudia Roach; Tyan L Carter; Erkang Fan; Paul A M Michels; Wim G J Hol
Analysis of the sequence motifs responsible for the interactions of peroxins 14 and 5, which are involved in glycosome biogenesis in Trypanosoma brucei.
Biochemistry 2003;42(37):10915-22.
2003: Xubo Hu; Kiet T Nguyen; Christophe Verlinde; Wim G J Hol; Dehua Pei
Structure-based design of a macrocyclic inhibitor for peptide deformylase.
Journal of medicinal chemistry 2003;46(18):3771-4.
2003: Juliette K Tinker; Jarrod L Erbe; Wim G J Hol; Randall K Holmes
Cholera holotoxin assembly requires a hydrophobic domain at the A-B5 interface: mutational analysis and development of an in vitro assembly system.
Infection and immunity 2003;71(7):4093-101.
2003: Jungwoo Choe; Daniel G Guerra; Paul A M Michels; Wim G J Hol
Leishmania mexicana glycerol-3-phosphate dehydrogenase showed conformational changes upon binding a bi-substrate adduct.
Journal of molecular biology 2003;329(2):335-49.
2003: Juliette Moyersoen; Jungwoo Choe; Abhinav Kumar; Frank Voncken; Wim G J Hol; Paul A M Michels
Characterization of Trypanosoma brucei PEX14 and its role in the import of glycosomal matrix proteins.
European journal of biochemistry / FEBS 2003;270(9):2059-67.
2003: Paulene M Quigley; Konstantin Korotkov; François Baneyx; Wim G J Hol
The 1.6-A crystal structure of the class of chaperones represented by Escherichia coli Hsp31 reveals a putative catalytic triad.
Proceedings of the National Academy of Sciences of the United States of America 2003;100(6):3137-42.
2003: Douglas R Davies; Heidrun Interthal; James J Champoux; Wim G J Hol
Crystal structure of a transition state mimic for Tdp1 assembled from vanadate, DNA, and a topoisomerase I-derived peptide.
Chemistry & biology 2003;10(2):139-47.
2002: Douglas R Davies; Heidrun Interthal; James J Champoux; Wim G J Hol
Insights into substrate binding and catalytic mechanism of human tyrosyl-DNA phosphodiesterase (Tdp1) from vanadate and tungstate-inhibited structures.
Journal of molecular biology 2002;324(5):917-32.
2002: Zhongsheng Zhang; Ethan A Merritt; Misol Ahn; Claudia Roach; Zheng Hou; Christophe Verlinde; Wim G J Hol; Erkang Fan
Solution and crystallographic studies of branched multivalent ligands that inhibit the receptor-binding of cholera toxin.
Journal of the American Chemical Society 2002;124(44):12991-8.
2002: Jungwoo Choe; Stephen Suresh; Goragot Wisedchaisri; Kevin J Kennedy; Michael H Gelb; Wim G J Hol
Anomalous differences of light elements in determining precise binding modes of ligands to glycerol-3-phosphate dehydrogenase.
Chemistry & biology 2002;9(11):1189-97.
2002: Sergio E Martinez; Albert Y Wu; Natalie A Glavas; Xiao-Bo Tang; Stewart Turley; Wim G J Hol; Joseph A Beavo
The two GAF domains in phosphodiesterase 2A have distinct roles in dimerization and in cGMP binding.
Proceedings of the National Academy of Sciences of the United States of America 2002;99(20):13260-5.
2002: Sergio E Martinez; Joseph A Beavo; Wim G J Hol
GAF domains: two-billion-year-old molecular switches that bind cyclic nucleotides.
Molecular interventions 2002;2(5):317-23.
2002: Ethan A Merritt; Zhongsheng Zhang; Jason Pickens; Misol Ahn; Wim G J Hol; Erkang Fan
Characterization and crystal structure of a high-affinity pentavalent receptor-binding inhibitor for cholera toxin and E. coli heat-labile enterotoxin.
Journal of the American Chemical Society 2002;124(30):8818-24.
2002: Michael H Gelb; Wim G J Hol
Parasitology. Drugs to combat tropical protozoan parasites.
Science (New York, N.Y.) 2002;297(5580):343-4.
2002: Abhinav Kumar; Kiet T Nguyen; Sumant Srivathsan; Brad Ornstein; Stewart Turley; Irwin Hirsh; Dehua Pei; Wim G J Hol
Crystals of peptide deformylase from Plasmodium falciparum reveal critical characteristics of the active site for drug design.
Structure (London, England : 1993) 2002;10(3):357-67.
2002: Jason Pickens; Ethan A Merritt; Misol Ahn; Christophe Verlinde; Wim G J Hol; Erkang Fan
Anchor-based design of improved cholera toxin and E. coli heat-labile enterotoxin receptor binding antagonists that display multiple binding modes.
Chemistry & biology 2002;9(2):215-24.
2002: Douglas R Davies; Heidrun Interthal; James J Champoux; Wim G J Hol
The crystal structure of human tyrosyl-DNA phosphodiesterase, Tdp1.
Structure (London, England : 1993) 2002;10(2):237-48.
2002: Youngsoo Kim; Sanggu Kim; Thomas N Earnest; Wim G J Hol
Precursor structure of cephalosporin acylase. Insights into autoproteolytic activation in a new N-terminal hydrolase family.
The Journal of biological chemistry 2002;277(4):2823-9.
2001: Youngsoo Kim; Wim G J Hol
Structure of cephalosporin acylase in complex with glutaryl-7-aminocephalosporanic acid and glutarate: insight into the basis of its substrate specificity.
Chemistry & biology 2001;8(12):1253-64.
2001: A Tholib Aman; S Fraser; Ethan A Merritt; C Rodigherio; M Kenny; Misol Ahn; Wim G J Hol; Neil A Williams; Wayne I Lencer; Timothy R Hirst
A mutant cholera toxin B subunit that binds GM1- ganglioside but lacks immunomodulatory or toxic activity.
Proceedings of the National Academy of Sciences of the United States of America 2001;98(15):8536-41.
2001: JC Bressi; Christophe Verlinde; AM Aronov; M L Shaw; S S Shin; Lananh N Nguyen; Stephen Suresh; Frederick S Buckner; Wesley C Van Voorhis; Irwin D Kuntz; Wim G J Hol; Michael H Gelb
Adenosine analogues as selective inhibitors of glyceraldehyde-3-phosphate dehydrogenase of Trypanosomatidae via structure-based drug design.
Journal of medicinal chemistry 2001;44(13):2080-93.
2001: Stephen Suresh; JC Bressi; Kevin J Kennedy; Christophe Verlinde; Michael H Gelb; Wim G J Hol
Conformational changes in Leishmania mexicana glyceraldehyde-3-phosphate dehydrogenase induced by designed inhibitors.
Journal of molecular biology 2001;309(2):423-35.
2001: E Pohl; Joanne Goranson-Siekierke; M K Choi; T Roosild; Randall K Holmes; Wim G J Hol
Structures of three diphtheria toxin repressor (DtxR) variants with decreased repressor activity.
Acta crystallographica. Section D, Biological crystallography 2001;57(Pt 5):619-27.
2001: A K Ghosh; Wim G J Hol; Erkang Fan
Solid-phase synthesis of N-acyl-N'-alkyl/aryl disubstituted guanidines.
The Journal of organic chemistry 2001;66(6):2161-4.
2001: Abhinav Kumar; Claudia Roach; Irwin Hirsh; Stewart Turley; S deWalque; Paul A M Michels; Wim G J Hol
An unexpected extended conformation for the third TPR motif of the peroxin PEX5 from Trypanosoma brucei.
Journal of molecular biology 2001;307(1):271-82.
2001: M D Feese; B P Ingason; Joanne Goranson-Siekierke; Randall K Holmes; Wim G J Hol
Crystal structure of the iron-dependent regulator from Mycobacterium tuberculosis at 2.0-A resolution reveals the Src homology domain 3-like fold and metal binding function of the third domain.
The Journal of biological chemistry 2001;276(8):5959-66.
2001: Christophe Verlinde; Véronique Hannaert; Casimir Blonski; Michèle Willson; Jacques Périé; Linda A Fothergill-Gilmore; Fred R Opperdoes; Michael H Gelb; Wim G J Hol; Paul A M Michels
Glycolysis as a target for the design of new anti-trypanosome drugs.
Drug resistance updates : reviews and commentaries in antimicrobial and anticancer chemotherapy 2001;4(1):50-65.
2001: Erkang Fan; Ethan A Merritt; Zhongsheng Zhang; Jason Pickens; Claudia Roach; Misol Ahn; Wim G J Hol
Exploration of the GM1 receptor-binding site of heat-labile enterotoxin and cholera toxin by phenyl-ring-containing galactose derivatives.
Acta crystallographica. Section D, Biological crystallography 2001;57(Pt 2):201-12.
2000: Erkang Fan; Ethan A Merritt; Christophe Verlinde; Wim G J Hol
AB(5) toxins: structures and inhibitor design.
Current opinion in structural biology 2000;10(6):680-6.
2000: JC Bressi; Jungwoo Choe; M T Hough; Frederick S Buckner; Wesley C Van Voorhis; Christophe Verlinde; Wim G J Hol; Michael H Gelb
Adenosine analogues as inhibitors of Trypanosoma brucei phosphoglycerate kinase: elucidation of a novel binding mode for a 2-amino-N(6)-substituted adenosine.
Journal of medicinal chemistry 2000;43(22):4135-50.
2000: Wim G J Hol
Structural genomics for science and society.
Nature structural biology 2000;7 Suppl():964-6.
2000: Youngsoo Kim; K Yoon; Y Khang; S Turley; Wim G J Hol
The 2.0 A crystal structure of cephalosporin acylase.
Structure (London, England : 1993) 2000;8(10):1059-68.
2000: A M Baca; Rachada Sirawaraporn; Stewart Turley; Worachart Sirawaraporn; Wim G J Hol
Crystal structure of Mycobacterium tuberculosis 7,8-dihydropteroate synthase in complex with pterin monophosphate: new insight into the enzymatic mechanism and sulfa-drug action.
Journal of molecular biology 2000;302(5):1193-212.
2000: D M Chudzik; Paul A M Michels; S de Walque; Wim G J Hol
Structures of type 2 peroxisomal targeting signals in two trypanosomatid aldolases.
Journal of molecular biology 2000;300(4):697-707.
2000: WE Minke; Jason Pickens; Ethan A Merritt; Erkang Fan; Christophe Verlinde; Wim G J Hol
Structure of m-carboxyphenyl-alpha-D-galactopyranoside complexed to heat-labile enterotoxin at 1.3 A resolution: surprising variations in ligand-binding modes.
Acta crystallographica. Section D, Biological crystallography 2000;56(Pt 7):795-804.
2000: MR Redinbo; James J Champoux; Wim G J Hol
Novel insights into catalytic mechanism from a crystal structure of human topoisomerase I in complex with DNA.
Biochemistry 2000;39(23):6832-40.
2000: Stephen Suresh; Stewart Turley; Fred R Opperdoes; Paul A M Michels; Wim G J Hol
A potential target enzyme for trypanocidal drugs revealed by the crystal structure of NAD-dependent glycerol-3-phosphate dehydrogenase from Leishmania mexicana.
Structure (London, England : 1993) 2000;8(5):541-52.
2000: A Aevarsson; Jacinta L Chuang; R Max Wynn; Stewart Turley; David T Chuang; Wim G J Hol
Crystal structure of human branched-chain alpha-ketoacid dehydrogenase and the molecular basis of multienzyme complex deficiency in maple syrup urine disease.
Structure (London, England : 1993) 2000;8(3):277-91.
2000: A M Baca; Wim G J Hol
Overcoming codon bias: a method for high-level overexpression of Plasmodium and other AT-rich parasite genes in Escherichia coli.
International journal for parasitology 2000;30(2):113-8.
2000: R Li; Rachada Sirawaraporn; Penchit Chitnumsub; Worachart Sirawaraporn; Jason M Wooden; F Athappilly; Stewart Turley; Wim G J Hol
Three-dimensional structure of M. tuberculosis dihydrofolate reductase reveals opportunities for the design of novel tuberculosis drugs.
Journal of molecular biology 2000;295(2):307-23.
1999: WE Minke; F Hong; Christophe Verlinde; Wim G J Hol; Erkang Fan
Using a galactose library for exploration of a novel hydrophobic pocket in the receptor binding site of the Escherichia coli heat-labile enterotoxin.
The Journal of biological chemistry 1999;274(47):33469-73.
1999: MR Redinbo; L Stewart; James J Champoux; Wim G J Hol
Structural flexibility in human topoisomerase I revealed in multiple non-isomorphous crystal structures.
Journal of molecular biology 1999;292(3):685-96.
1999: E Pohl; Randall K Holmes; Wim G J Hol
Crystal structure of a cobalt-activated diphtheria toxin repressor-DNA complex reveals a metal-binding SH3-like domain.
Journal of molecular biology 1999;292(3):653-67.
1999: D J Diller; M R Redinbo; E Pohl; Wim G J Hol
A database method for automated map interpretation in protein crystallography.
Proteins 1999;36(4):526-41.
1999: D J Diller; E Pohl; M R Redinbo; B T Hovey; Wim G J Hol
A rapid method for positioning small flexible molecules, nucleic acids, and large protein fragments in experimental electron density maps.
Proteins 1999;36(4):512-25.
1999: A Aevarsson; K Seger; Stewart Turley; J R Sokatch; Wim G J Hol
Crystal structure of 2-oxoisovalerate and dehydrogenase and the architecture of 2-oxo acid dehydrogenase multienzyme complexes.
Nature structural biology 1999;6(8):785-92.
1999: WE Minke; D J Diller; Wim G J Hol; Christophe Verlinde
The role of waters in docking strategies with incremental flexibility for carbohydrate derivatives: heat-labile enterotoxin, a multivalent test case.
Journal of medicinal chemistry 1999;42(10):1778-88.
1999: WE Minke; Claudia Roach; Wim G J Hol; Christophe Verlinde
Structure-based exploration of the ganglioside GM1 binding sites of Escherichia coli heat-labile enterotoxin and cholera toxin for the discovery of receptor antagonists.
Biochemistry 1999;38(18):5684-92.
1999: AM Aronov; Stephen Suresh; Frederick S Buckner; Wesley C Van Voorhis; Christophe Verlinde; Fred R Opperdoes; Wim G J Hol; Michael H Gelb
Structure-based design of submicromolar, biologically active inhibitors of trypanosomatid glyceraldehyde-3-phosphate dehydrogenase.
Proceedings of the National Academy of Sciences of the United States of America 1999;96(8):4273-8.
1999: Joanne Goranson-Siekierke; E Pohl; Wim G J Hol; Randall K Holmes
Anion-coordinating residues at binding site 1 are essential for the biological activity of the diphtheria toxin repressor.
Infection and immunity 1999;67(4):1806-11.
1999: D J Diller; Wim G J Hol
An accurate numerical model for calculating the equilibration rate of a hanging-drop experiment.
Acta crystallographica. Section D, Biological crystallography 1999;55(Pt 3):656-63.
1999: T Izard; A Aevarsson; M D Allen; Adrie Westphal; R N Perham; Aart de Kok; Wim G J Hol
Principles of quasi-equivalence and Euclidean geometry govern the assembly of cubic and dodecahedral cores of pyruvate dehydrogenase complexes.
Proceedings of the National Academy of Sciences of the United States of America 1999;96(4):1240-5.
1999: MR Redinbo; James J Champoux; Wim G J Hol
Structural insights into the function of type IB topoisomerases.
Current opinion in structural biology 1999;9(1):29-36.
1999: B T Hovey; Christophe Verlinde; Ethan A Merritt; Wim G J Hol
Structure-based discovery of a pore-binding ligand: towards assembly inhibitors for cholera and related AB5 toxins.
Journal of molecular biology 1999;285(3):1169-78.
1999: E Pohl; Randall K Holmes; Wim G J Hol
Crystal structure of the iron-dependent regulator (IdeR) from Mycobacterium tuberculosis shows both metal binding sites fully occupied.
Journal of molecular biology 1999;285(3):1145-56.
1999: Focco Van Den Akker; Wim G J Hol
Difference density quality (DDQ): a method to assess the global and local correctness of macromolecular crystal structures.
Acta crystallographica. Section D, Biological crystallography 1999;55(Pt 1):206-18.
1998: AM Aronov; Christophe Verlinde; Wim G J Hol; Michael H Gelb
Selective tight binding inhibitors of trypanosomal glyceraldehyde-3-phosphate dehydrogenase via structure-based drug design.
Journal of medicinal chemistry 1998;41(24):4790-9.
1998: IK Feil; A A Platas; Focco Van Den Akker; R Reddy; Ethan A Merritt; D R Storm; Wim G J Hol
Stepwise transplantation of an active site loop between heat-labile enterotoxins LT-II and LT-I and characterization of the obtained hybrid toxins.
Protein engineering 1998;11(11):1103-9.
1998: Ethan A Merritt; P Kuhn; S Sarfaty; Jarrod L Erbe; Randall K Holmes; Wim G J Hol
The 1.25 A resolution refinement of the cholera toxin B-pentamer: evidence of peptide backbone strain at the receptor-binding site.
Journal of molecular biology 1998;282(5):1043-59.
1998: E Pohl; Randall K Holmes; Wim G J Hol
Motion of the DNA-binding domain with respect to the core of the diphtheria toxin repressor (DtxR) revealed in the crystal structures of apo- and holo-DtxR.
The Journal of biological chemistry 1998;273(35):22420-7.
1998: BE Bernstein; D M Williams; JC Bressi; P Kuhn; Michael H Gelb; G M Blackburn; Wim G J Hol
A bisubstrate analog induces unexpected conformational changes in phosphoglycerate kinase from Trypanosoma brucei.
Journal of molecular biology 1998;279(5):1137-48.
1998: J I Yeh; Wim G J Hol
A flash-annealing technique to improve diffraction limits and lower mosaicity in crystals of glycerol kinase.
Acta crystallographica. Section D, Biological crystallography 1998;54(Pt 3):479-80.
1998: H Kim; Wim G J Hol
Crystal structure of Leishmania mexicana glycosomal glyceraldehyde-3-phosphate dehydrogenase in a new crystal form confirms the putative physiological active site structure.
Journal of molecular biology 1998;278(1):5-11.
1998: B E Bernstein; Wim G J Hol
Crystal structures of substrates and products bound to the phosphoglycerate kinase active site reveal the catalytic mechanism.
Biochemistry 1998;37(13):4429-36.
1998: L Stewart; MR Redinbo; X Qiu; Wim G J Hol; James J Champoux
A model for the mechanism of human topoisomerase I.
Science (New York, N.Y.) 1998;279(5356):1534-41.
1998: MR Redinbo; L Stewart; P Kuhn; James J Champoux; Wim G J Hol
Crystal structures of human topoisomerase I in covalent and noncovalent complexes with DNA.
Science (New York, N.Y.) 1998;279(5356):1504-13.
1998: BE Bernstein; Paul A M Michels; H Kim; Philip H Petra; Wim G J Hol
The importance of dynamic light scattering in obtaining multiple crystal forms of Trypanosoma brucei PGK.
Protein science : a publication of the Protein Society 1998;7(2):504-7.
1998: Gabby Rudenko; Erik J Bonten; Wim G J Hol; Alessandra d'Azzo
The atomic model of the human protective protein/cathepsin A suggests a structural basis for galactosialidosis.
Proceedings of the National Academy of Sciences of the United States of America 1998;95(2):621-5.
1997: Focco Van Den Akker; M Pizza; R Rappuoli; Wim G J Hol
Crystal structure of a non-toxic mutant of heat-labile enterotoxin, which is a potent mucosal adjuvant.
Protein science : a publication of the Protein Society 1997;6(12):2650-4.
1997: Focco Van Den Akker; IK Feil; Claudia Roach; A A Platas; Ethan A Merritt; Wim G J Hol
Crystal structure of heat-labile enterotoxin from Escherichia coli with increased thermostability introduced by an engineered disulfide bond in the A subunit.
Protein science : a publication of the Protein Society 1997;6(12):2644-9.
1997: Ethan A Merritt; S Sarfaty; IK Feil; Wim G J Hol
Structural foundation for the design of receptor antagonists targeting Escherichia coli heat-labile enterotoxin.
Structure (London, England : 1993) 1997;5(11):1485-99.
1997: B E Bernstein; Wim G J Hol
Probing the limits of the molecular replacement method: the case of Trypanosoma brucei phosphoglycerate kinase.
Acta crystallographica. Section D, Biological crystallography 1997;53(Pt 6):756-64.
1997: Ethan A Merritt; S Sarfaty; Michael G Jobling; T Chang; Randall K Holmes; Timothy R Hirst; Wim G J Hol
Structural studies of receptor binding by cholera toxin mutants.
Protein science : a publication of the Protein Society 1997;6(7):1516-28.
1997: E Pohl; X Qui; L M Must; Randall K Holmes; Wim G J Hol
Comparison of high-resolution structures of the diphtheria toxin repressor in complex with cobalt and zinc at the cation-anion binding site.
Protein science : a publication of the Protein Society 1997;6(5):1114-8.
1997: T Izard; S Sarfaty; Adrie Westphal; Aart de Kok; Wim G J Hol
Improvement of diffraction quality upon rehydration of dehydrated icosahedral Enterococcus faecalis pyruvate dehydrogenase core crystals.
Protein science : a publication of the Protein Society 1997;6(4):913-5.
1997: BE Bernstein; Paul A M Michels; Wim G J Hol
Synergistic effects of substrate-induced conformational changes in phosphoglycerate kinase activation.
Nature 1997;385(6613):275-8.
1996: L de Haan; Willem Ronald Verweij; I K Feil; T H Lijnema; Wim G J Hol; E Agsteribbe; Jan Wilschut
Mutants of the Escherichia coli heat-labile enterotoxin with reduced ADP-ribosylation activity or no activity retain the immunogenic properties of the native holotoxin.
Infection and immunity 1996;64(12):5413-6.
1996: B Hazes; K A Magnus; Kor H Kalk; C Bonaventura; Wim G J Hol
Nitrate binding to Limulus polyphemus subunit type II hemocyanin and its functional implications.
Journal of molecular biology 1996;262(4):532-41.
1996: X Qiu; E Pohl; Randall K Holmes; Wim G J Hol
High-resolution structure of the diphtheria toxin repressor complexed with cobalt and manganese reveals an SH3-like third domain and suggests a possible role of phosphate as co-corepressor.
Biochemistry 1996;35(38):12292-302.
1996: Gabby Rudenko; Erik J Bonten; Alessandra d'Azzo; Wim G J Hol
Structure determination of the human protective protein: twofold averaging reveals the three-dimensional structure of a domain which was entirely absent in the initial model.
Acta crystallographica. Section D, Biological crystallography 1996;52(Pt 5):923-36.
1996: J I Yeh; Al Claiborne; Wim G J Hol
Structure of the native cysteine-sulfenic acid redox center of enterococcal NADH peroxidase refined at 2.8 A resolution.
Biochemistry 1996;35(31):9951-7.
1996: Focco Van Den Akker; S Sarfaty; E M Twiddy; T D Connell; Randall K Holmes; Wim G J Hol
Crystal structure of a new heat-labile enterotoxin, LT-IIb.
Structure (London, England : 1993) 1996;4(6):665-78.
1996: Focco Van Den Akker; E Steensma; Wim G J Hol
Tumor marker disaccharide D-Gal-beta 1, 3-GalNAc complexed to heat-labile enterotoxin from Escherichia coli.
Protein science : a publication of the Protein Society 1996;5(6):1184-8.
1996: I K Feil; R Reddy; L de Haan; Ethan A Merritt; Focco Van Den Akker; D R Storm; Wim G J Hol
Protein engineering studies of A-chain loop 47-56 of Escherichia coli heat-labile enterotoxin point to a prominent role of this loop for cytotoxicity.
Molecular microbiology 1996;20(4):823-32.
1996: V Mainfroid; S C Mande; Wim G J Hol; Joseph A Martial; K Goraj
Stabilization of human triosephosphate isomerase by improvement of the stability of individual alpha-helices in dimeric as well as monomeric forms of the protein.
Biochemistry 1996;35(13):4110-7.
1996: V Mainfroid; P Terpstra; M Beauregard; Jean-Marie Frère; S C Mande; Wim G J Hol; Joseph A Martial; K Goraj
Three hTIM mutants that provide new insights on why TIM is a dimer.
Journal of molecular biology 1996;257(2):441-56.
1996: S S Mande; S Sarfaty; M D Allen; R N Perham; Wim G J Hol
Protein-protein interactions in the pyruvate dehydrogenase multienzyme complex: dihydrolipoamide dehydrogenase complexed with the binding domain of dihydrolipoamide acetyltransferase.
Structure (London, England : 1993) 1996;4(3):277-86.
1996: S C Mande; V Mehra; B R Bloom; Wim G J Hol
Structure of the heat shock protein chaperonin-10 of Mycobacterium leprae.
Science (New York, N.Y.) 1996;271(5246):203-7.
1996: Alexey Teplyakov; P Gros; Wim G J Hol
Crystallographic study of eglin-C binding to thermitase.
Advances in experimental medicine and biology 1996;379():5-9.
1995: L F Delboni; S C Mande; Francoise Rentier-Delrue; V Mainfroid; S Turley; Frédéric Vellieux; Joseph A Martial; Wim G J Hol
Crystal structure of recombinant triosephosphate isomerase from Bacillus stearothermophilus. An analysis of potential thermostability factors in six isomerases with known three-dimensional structures points to the importance of hydrophobic interactions.
Protein science : a publication of the Protein Society 1995;4(12):2594-604.
1995: H Kim; I K Feil; Christophe Verlinde; P H Petra; Wim G J Hol
Crystal structure of glycosomal glyceraldehyde-3-phosphate dehydrogenase from Leishmania mexicana: implications for structure-based drug design and a new position for the inorganic phosphate binding site.
Biochemistry 1995;34(46):14975-86.
1995: Gabby Rudenko; Erik J Bonten; Alessandra d'Azzo; Wim G J Hol
Three-dimensional structure of the human 'protective protein': structure of the precursor form suggests a complex activation mechanism.
Structure (London, England : 1993) 1995;3(11):1249-59.
1995: Serge Van Calenbergh; Christophe Verlinde; J Soenens; A De Bruyn; Mia callens; Norbert Blaton; Oswald M Peeters; Jef Rozenski; Wim G J Hol; Piet Herdewijn
Synthesis and structure-activity relationships of analogs of 2'-deoxy-2'-(3-methoxybenzamido)adenosine, a selective inhibitor of trypanosomal glycosomal glyceraldehyde-3-phosphate dehydrogenase.
Journal of medicinal chemistry 1995;38(19):3838-49.
1995: Focco Van Den Akker; Ethan A Merritt; M Pizza; M Domenighini; R Rappuoli; Wim G J Hol
The Arg7Lys mutant of heat-labile enterotoxin exhibits great flexibility of active site loop 47-56 of the A subunit.
Biochemistry 1995;34(35):10996-1004.
1995: Frédéric Vellieux; Janos Hajdu; Wim G J Hol
Refined 3.2 A structure of glycosomal holo glyceraldehyde phosphate dehydrogenase from Trypanosoma brucei brucei.
Acta crystallographica. Section D, Biological crystallography 1995;51(Pt 4):575-89.
1995: Ethan A Merritt; S Sarfaty; T T Chang; L M Palmer; Michael G Jobling; Randall K Holmes; Wim G J Hol
Surprising leads for a cholera toxin receptor-binding antagonist: crystallographic studies of CTB mutants.
Structure (London, England : 1993) 1995;3(6):561-70.
1995: S S Mande; Derek Parsonage; Al Claiborne; Wim G J Hol
Crystallographic analyses of NADH peroxidase Cys42Ala and Cys42Ser mutants: active site structures, mechanistic implications, and an unusual environment of Arg 303.
Biochemistry 1995;34(21):6985-92.
1995: Menachem Shoham; T Scherf; Jacob Anglister; M Levitt; E A Merritt; Wim G J Hol
Structural diversity in a conserved cholera toxin epitope involved in ganglioside binding.
Protein science : a publication of the Protein Society 1995;4(5):841-8.
1995: Holly Miller; S S Mande; Derek Parsonage; S Sarfaty; Wim G J Hol; Al Claiborne
An L40C mutation converts the cysteine-sulfenic acid redox center in enterococcal NADH peroxidase to a disulfide.
Biochemistry 1995;34(15):5180-90.
1995: J Hendle; Andrea Mattevi; Adrie Westphal; J Spee; Aart de Kok; Alexey Teplyakov; Wim G J Hol
Crystallographic and enzymatic investigations on the role of Ser558, His610, and Asn614 in the catalytic mechanism of Azotobacter vinelandii dihydrolipoamide acetyltransferase (E2p).
Biochemistry 1995;34(13):4287-98.
1995: Ethan A Merritt; S Sarfaty; M Pizza; M Domenighini; R Rappuoli; Wim G J Hol
Mutation of a buried residue causes loss of activity but no conformational change in the heat-labile enterotoxin of Escherichia coli.
Nature structural biology 1995;2(4):269-72.
1995: Ethan A Merritt; Wim G J Hol
AB5 toxins.
Current opinion in structural biology 1995;5(2):165-71.
1995: X Qiu; Christophe Verlinde; S Zhang; M P Schmitt; Randall K Holmes; Wim G J Hol
Three-dimensional structure of the diphtheria toxin repressor in complex with divalent cation co-repressors.
Structure (London, England : 1993) 1995;3(1):87-100.
1994: Mariagrazia Pizza; M R Fontana; Marzia M Giuliani; M Domenighini; C Magagnoli; V Giannelli; D Nucci; Wim G J Hol; Roberto Manetti; Rino Rappuoli
A genetically detoxified derivative of heat-labile Escherichia coli enterotoxin induces neutralizing antibodies against the A subunit.
The Journal of experimental medicine 1994;180(6):2147-53.
1994: Christophe Verlinde; Mia callens; Serge Van Calenbergh; Arthur Van Aerschot; Piet Herdewijn; Véronique Hannaert; Paul A M Michels; Fred R Opperdoes; Wim G J Hol
Selective inhibition of trypanosomal glyceraldehyde-3-phosphate dehydrogenase by protein structure-based design: toward new drugs for the treatment of sleeping sickness.
Journal of medicinal chemistry 1994;37(21):3605-13.
1994: Christophe Verlinde; Ethan A Merritt; Focco Van Den Akker; H Kim; I Feil; L F Delboni; S C Mande; S Sarfaty; P H Petra; Wim G J Hol
Protein crystallography and infectious diseases.
Protein science : a publication of the Protein Society 1994;3(10):1670-86.
1994: Mariagrazia Pizza; M Domenighini; Wim G J Hol; V Giannelli; M R Fontana; Marzia M Giuliani; C Magagnoli; S Peppoloni; Roberto Manetti; Rino Rappuoli
Probing the structure-activity relationship of Escherichia coli LT-A by site-directed mutagenesis.
Molecular microbiology 1994;14(1):51-60.
1994: H A Schreuder; Andrea Mattevi; Galina Obmolova; Kor H Kalk; Wim G J Hol; F J van der Bolt; Willem J H van Berkel
Crystal structures of wild-type p-hydroxybenzoate hydroxylase complexed with 4-aminobenzoate,2,4-dihydroxybenzoate, and 2-hydroxy-4-aminobenzoate and of the Tyr222Ala mutant complexed with 2-hydroxy-4-aminobenzoate. Evidence for a proton channel and a new binding mode of the flavin ring.
Biochemistry 1994;33(33):10161-70.
1994: Ethan A Merritt; T K Sixma; Kor H Kalk; B A van Zanten; Wim G J Hol
Galactose-binding site in Escherichia coli heat-labile enterotoxin (LT) and cholera toxin (CT).
Molecular microbiology 1994;13(4):745-53.
1994: K A Magnus; B Hazes; H Ton-That; C Bonaventura; J Bonaventura; Wim G J Hol
Crystallographic analysis of oxygenated and deoxygenated states of arthropod hemocyanin shows unusual differences.
Proteins 1994;19(4):302-9.
1994: Christophe Verlinde; Wim G J Hol
Structure-based drug design: progress, results and challenges.
Structure (London, England : 1993) 1994;2(7):577-87.
1994: S C Mande; V Mainfroid; Kor H Kalk; K Goraj; Joseph A Martial; Wim G J Hol
Crystal structure of recombinant human triosephosphate isomerase at 2.8 A resolution. Triosephosphate isomerase-related human genetic disorders and comparison with the trypanosomal enzyme.
Protein science : a publication of the Protein Society 1994;3(5):810-21.
1994: Ethan A Merritt; S Sarfaty; Focco Van Den Akker; C L'Hoir; Joseph A Martial; Wim G J Hol
Crystal structure of cholera toxin B-pentamer bound to receptor GM1 pentasaccharide.
Protein science : a publication of the Protein Society 1994;3(2):166-75.
1994: Ethan A Merritt; S E Pronk; T K Sixma; Kor H Kalk; B A van Zanten; Wim G J Hol
Structure of partially-activated E. coli heat-labile enterotoxin (LT) at 2.6 A resolution.
FEBS letters 1994;337(1):88-92.
1994: H A Schreuder; B de Boer; R Dijkema; JW Mulders; H J Theunissen; PD Grootenhuis; Wim G J Hol
The intact and cleaved human antithrombin III complex as a model for serpin-proteinase interactions.
Nature structural biology 1994;1(1):48-54.
1993: H Schreuder; A Arkema; B de Boer; Kor H Kalk; R Dijkema; JW Mulders; H Theunissen; Wim G J Hol
Crystallization and preliminary crystallographic analysis of antistasin, a leech-derived inhibitor of blood coagulation factor Xa.
Journal of molecular biology 1993;231(4):1137-8.
1993: Andrea Mattevi; Galina Obmolova; Kor H Kalk; Willem J H van Berkel; Wim G J Hol
Three-dimensional structure of lipoamide dehydrogenase from Pseudomonas fluorescens at 2.8 A resolution. Analysis of redox and thermostability properties.
Journal of molecular biology 1993;230(4):1200-15.
1993: Andrea Mattevi; Galina Obmolova; Kor H Kalk; Adrie Westphal; Aart de Kok; Wim G J Hol
Refined crystal structure of the catalytic domain of dihydrolipoyl transacetylase (E2p) from Azotobacter vinelandii at 2.6 A resolution.
Journal of molecular biology 1993;230(4):1183-99.
1993: Andrea Mattevi; Galina Obmolova; Kor H Kalk; Alexey Teplyakov; Wim G J Hol
Crystallographic analysis of substrate binding and catalysis in dihydrolipoyl transacetylase (E2p).
Biochemistry 1993;32(15):3887-901.
1993: T K Sixma; Kor H Kalk; B A van Zanten; Z Dauter; J Kingma; Bernard Witholt; Wim G J Hol
Refined structure of Escherichia coli heat-labile enterotoxin, a close relative of cholera toxin.
Journal of molecular biology 1993;230(3):890-918.
1993: B Hazes; K A Magnus; C Bonaventura; J Bonaventura; Z Dauter; Kor H Kalk; Wim G J Hol
Crystal structure of deoxygenated Limulus polyphemus subunit II hemocyanin at 2.18 A resolution: clues for a mechanism for allosteric regulation.
Protein science : a publication of the Protein Society 1993;2(4):597-619.
1993: T K Sixma; P E Stein; Wim G J Hol; Randy J Read
Comparison of the B-pentamers of heat-labile enterotoxin and verotoxin-1: two structures with remarkable similarity and dissimilarity.
Biochemistry 1993;32(1):191-8.
1993: Francoise Rentier-Delrue; S C Mande; S Moyens; P Terpstra; V Mainfroid; K Goraj; M Lion; Wim G J Hol; Joseph A Martial
Cloning and overexpression of the triosephosphate isomerase genes from psychrophilic and thermophilic bacteria. Structural comparison of the predicted protein sequences.
Journal of molecular biology 1993;229(1):85-93.
1993: H Schreuder; B de Boer; S Pronk; Wim G J Hol; R Dijkema; JW Mulders; H Theunissen
Crystallization and preliminary X-ray analysis of human antithrombin III.
Journal of molecular biology 1993;229(1):249-50.
1992: Christophe Verlinde; C J Witmans; Tjaard Pijning; Kor H Kalk; Wim G J Hol; Mia callens; Fred R Opperdoes
Structure of the complex between trypanosomal triosephosphate isomerase and N-hydroxy-4-phosphono-butanamide: binding at the active site despite an "open" flexible loop conformation.
Protein science : a publication of the Protein Society 1992;1(12):1578-84.
1992: H A Schreuder; J M van der Laan; M B Swarte; Kor H Kalk; Wim G J Hol; J Drenth
Crystal structure of the reduced form of p-hydroxybenzoate hydroxylase refined at 2.3 A resolution.
Proteins 1992;14(2):178-90.
1992: Andrea Mattevi; Galina Obmolova; J R Sokatch; C Betzel; Wim G J Hol
The refined crystal structure of Pseudomonas putida lipoamide dehydrogenase complexed with NAD+ at 2.45 A resolution.
Proteins 1992;13(4):336-51.
1992: T K Sixma; A Aguirre; A C Terwisscha van Scheltinga; E S Wartna; Kor H Kalk; Wim G J Hol
Heat-labile enterotoxin crystal forms with variable A/B5 orientation. Analysis of conformational flexibility.
FEBS letters 1992;305(2):81-5.
1992: Christophe Verlinde; Gabby Rudenko; Wim G J Hol
In search of new lead compounds for trypanosomiasis drug design: a protein structure-based linked-fragment approach.
Journal of computer-aided molecular design 1992;6(2):131-47.
1992: Andrea Mattevi; Galina Obmolova; E Schulze; Kor H Kalk; Adrie Westphal; Aart de Kok; Wim G J Hol
Atomic structure of the cubic core of the pyruvate dehydrogenase multienzyme complex.
Science (New York, N.Y.) 1992;255(5051):1544-50.
1992: B Hazes; Wim G J Hol
Comparison of the hemocyanin beta-barrel with other Greek key beta-barrels: possible importance of the "beta-zipper" in protein structure and folding.
Proteins 1992;12(3):278-98.
1992: T K Sixma; S E Pronk; Kor H Kalk; B A van Zanten; Albert Berghuis; Wim G J Hol
Lactose binding to heat-labile enterotoxin revealed by X-ray crystallography.
Nature 1992;355(6360):561-4.
1992: T K Sixma; A C Terwisscha van Scheltinga; Kor H Kalk; K Zhou; E S Wartna; Wim G J Hol
X-ray studies reveal lanthanide binding sites at the A/B5 interface of E. coli heat labile enterotoxin.
FEBS letters 1992;297(1-2):179-82.
1992: P Gros; Alexey Teplyakov; Wim G J Hol
Effects of eglin-c binding to thermitase: three-dimensional structure comparison of native thermitase and thermitase eglin-c complexes.
Proteins 1992;12(1):63-74.
1991: E Schulze; Adrie Westphal; Galina Obmolova; Andrea Mattevi; Wim G J Hol; Aart de Kok
The catalytic domain of the dihydrolipoyl transacetylase component of the pyruvate dehydrogenase complex from Azotobacter vinelandii and Escherichia coli. Expression, purification, properties and preliminary X-ray analysis.
European journal of biochemistry / FEBS 1991;201(3):561-8.
1991: M E Noble; Christophe Verlinde; H Groendijk; Kor H Kalk; Rik K Wierenga; Wim G J Hol
Crystallographic and molecular modeling studies on trypanosomal triosephosphate isomerase: a critical assessment of the predicted and observed structures of the complex with 2-phosphoglycerate.
Journal of medicinal chemistry 1991;34(9):2709-18.
1991: Rik K Wierenga; M E Noble; G Vriend; S Nauche; Wim G J Hol
Refined 1.83 A structure of trypanosomal triosephosphate isomerase crystallized in the presence of 2.4 M-ammonium sulphate. A comparison with the structure of the trypanosomal triosephosphate isomerase-glycerol-3-phosphate complex.
Journal of molecular biology 1991;220(4):995-1015.
1991: Andrea Mattevi; Abraham Schierbeek; Wim G J Hol
Refined crystal structure of lipoamide dehydrogenase from Azotobacter vinelandii at 2.2 A resolution. A comparison with the structure of glutathione reductase.
Journal of molecular biology 1991;220(4):975-94.
1991: T K Sixma; S E Pronk; Kor H Kalk; E S Wartna; B A van Zanten; Bernard Witholt; Wim G J Hol
Crystal structure of a cholera toxin-related heat-labile enterotoxin from E. coli.
Nature 1991;351(6325):371-7.
1991: Christophe Verlinde; M E Noble; Kor H Kalk; H Groendijk; Rik K Wierenga; Wim G J Hol
Anion binding at the active site of trypanosomal triosephosphate isomerase. Monohydrogen phosphate does not mimic sulphate.
European journal of biochemistry / FEBS 1991;198(1):53-7.
1991: P Gros; Kor H Kalk; Wim G J Hol
Calcium binding to thermitase. Crystallographic studies of thermitase at 0, 5, and 100 mM calcium.
The Journal of biological chemistry 1991;266(5):2953-61.
1991: M E Noble; Rik K Wierenga; A M Lambeir; Fred R Opperdoes; A M Thunnissen; Kor H Kalk; H Groendijk; Wim G J Hol
The adaptability of the active site of trypanosomal triosephosphate isomerase as observed in the crystal structures of three different complexes.
Proteins 1991;10(1):50-69.
1991: Rik K Wierenga; M E Noble; J P Postma; H Groendijk; Kor H Kalk; Wim G J Hol; Fred R Opperdoes
The crystal structure of the "open" and the "closed" conformation of the flexible loop of trypanosomal triosephosphate isomerase.
Proteins 1991;10(1):33-49.
1991: K A Magnus; Eaton E Lattman; A Volbeda; Wim G J Hol
Hexamers of subunit II from Limulus hemocyanin (a 48-mer) have the same quaternary structure as whole Panulirus hemocyanin molecules.
Proteins 1991;9(4):240-7.
1990: K Eschrich; Willem J H van Berkel; Adrie Westphal; Aart de Kok; Andrea Mattevi; Galina Obmolova; Kor H Kalk; Wim G J Hol
Engineering of microheterogeneity-resistant p-hydroxybenzoate hydroxylase from Pseudomonas fluorescens.
FEBS letters 1990;277(1-2):197-9.
1990: Frédéric Vellieux; Kor H Kalk; J Drenth; Wim G J Hol
Structure determination of quinoprotein methylamine dehydrogenase from Thiobacillus versutus.
Acta crystallographica. Section B, Structural science 1990;46 ( Pt 6)():806-23.
1990: P Gros; Wilfred F van Gunsteren; Wim G J Hol
Inclusion of thermal motion in crystallographic structures by restrained molecular dynamics.
Science (New York, N.Y.) 1990;249(4973):1149-52.
1990: H A Schreuder; Wim G J Hol; J Drenth
Analysis of the active site of the flavoprotein p-hydroxybenzoate hydroxylase and some ideas with respect to its reaction mechanism.
Biochemistry 1990;29(12):3101-8.
1989: P Gros; C Betzel; Z Dauter; K S Wilson; Wim G J Hol
Molecular dynamics refinement of a thermitase-eglin-c complex at 1.98 A resolution and comparison of two crystal forms that differ in calcium content.
Journal of molecular biology 1989;210(2):347-67.
1989: P Gros; M Fujinaga; B W Dijkstra; Kor H Kalk; Wim G J Hol
Crystallographic refinement by incorporation of molecular dynamics: thermostable serine protease thermitase complexed with eglin c.
Acta crystallographica. Section B, Structural science 1989;45 ( Pt 5)():488-99.
1989: A Volbeda; Wim G J Hol
Crystal structure of hexameric haemocyanin from Panulirus interruptus refined at 3.2 A resolution.
Journal of molecular biology 1989;209(2):249-79.
1989: J M van der Laan; H A Schreuder; M B Swarte; Rik K Wierenga; Kor H Kalk; Wim G J Hol; J Drenth
The coenzyme analogue adenosine 5-diphosphoribose displaces FAD in the active site of p-hydroxybenzoate hydroxylase. An x-ray crystallographic investigation.
Biochemistry 1989;28(18):7199-205.
1989: H A Schreuder; P A Prick; Rik K Wierenga; Gert Vriend; K S Wilson; Wim G J Hol; J Drenth
Crystal structure of the p-hydroxybenzoate hydroxylase-substrate complex refined at 1.9 A resolution. Analysis of the enzyme-substrate and enzyme-product complexes.
Journal of molecular biology 1989;208(4):679-96.
1989: Frédéric Vellieux; F Huitema; H Groendijk; Kor H Kalk; J F Jzn; J A Jongejan; Johannis A Duine; K Petratos; J Drenth; Wim G J Hol
Structure of quinoprotein methylamine dehydrogenase at 2.25 A resolution.
The EMBO journal 1989;8(8):2171-8.
1989: A Volbeda; M C Feiters; M G Vincent; E Bouwman; B Dobson; Kor H Kalk; Jan Reedijk; Wim G J Hol
Spectroscopic investigations of Panulirus interruptus hemocyanin in the crystalline state.
European journal of biochemistry / FEBS 1989;181(3):669-73.
1989: A Volbeda; Wim G J Hol
Pseudo 2-fold symmetry in the copper-binding domain of arthropodan haemocyanins. Possible implications for the evolution of oxygen transport proteins.
Journal of molecular biology 1989;206(3):531-46.
1989: Abraham Schierbeek; M B Swarte; Bauke W Dijkstra; Gert Vriend; Randy J Read; Wim G J Hol; J Drenth; C Betzel
X-ray structure of lipoamide dehydrogenase from Azotobacter vinelandii determined by a combination of molecular and isomorphous replacement techniques.
Journal of molecular biology 1989;206(2):365-79.
1989: J M Van der Laan; M B Swarte; H Groendijk; Wim G J Hol; J Drenth
The influence of purification and protein heterogeneity on the crystallization of p-hydroxybenzoate hydroxylase.
European journal of biochemistry / FEBS 1989;179(3):715-24.
1989: Ida Van der klei; C L Lawson; Henriëtte J Rozeboom; Bauke W Dijkstra; Marten Veenhuis; W Harder; Wim G J Hol
Use of electron microscopy in the examination of lattice defects in crystals of alcohol oxidase.
FEBS letters 1989;244(1):213-6.
1989: H A Kelders; Kor H Kalk; Wim G J Hol
Crystallization of thermitase, a thermostable subtilisin, from a sodium formate solution by means of an automated procedure.
Journal of molecular biology 1989;205(3):615-6.
1988: P J Kooystra; Kor H Kalk; Wim G J Hol
Soaking in Cs2SO4 reveals a caesium-aromatic interaction in bovine-liver rhodanese.
European journal of biochemistry / FEBS 1988;177(2):345-9.
1988: H A Schreuder; Wim G J Hol; J Drenth
Molecular modeling reveals the possible importance of a carbonyl oxygen binding pocket for the catalytic mechanism of p-hydroxybenzoate hydroxylase.
The Journal of biological chemistry 1988;263(7):3131-6.
1988: H A Schreuder; J M van der Laan; Wim G J Hol; J Drenth
Crystal structure of p-hydroxybenzoate hydroxylase complexed with its reaction product 3,4-dihydroxybenzoate.
Journal of molecular biology 1988;199(4):637-48.
1988: A Volbeda; Wim G J Hol
Structure of arthropodan hemocyanin.
Progress in clinical and biological research 1988;274():291-307.
1987: Rik K Wierenga; Kor H Kalk; Wim G J Hol
Structure determination of the glycosomal triosephosphate isomerase from Trypanosoma brucei brucei at 2.4 A resolution.
Journal of molecular biology 1987;198(1):109-21.
1987: H A Kelders; Kor H Kalk; P Gros; Wim G J Hol
Automated protein crystallization and a new crystal form of a subtilisin:eglin complex.
Protein engineering 1987;1(4):301-3.
1987: Randy J Read; Rik K Wierenga; H Groendijk; Wim G J Hol; A Lambeir; Fred R Opperdoes
Preliminary crystallographic studies of glycosomal glyceraldehyde phosphate dehydrogenase from Trypanosoma brucei brucei.
Journal of molecular biology 1987;194(3):573-5.
1986: Frédéric Vellieux; J Frank; M B Swarte; H Groendijk; Johannis A Duine; J Drenth; Wim G J Hol
Purification, crystallization and preliminary X-ray investigation of quinoprotein methylamine dehydrogenase from Thiobacillus versutus.
European journal of biochemistry / FEBS 1986;154(2):383-6.
1985: S E Pronk; H Hofstra; H Groendijk; J Kingma; M B Swarte; F Dorner; J Drenth; Wim G J Hol; Bernard Witholt
Heat-labile enterotoxin of Escherichia coli. Characterization of different crystal forms.
The Journal of biological chemistry 1985;260(25):13580-4.