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Stephen Hughes

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Mathieu Métifiot; Steven J Smith; Xue Zhi Zhao; Terrence R Burke; Peter Cherepanov; Stephen Hare; Stephen H Hughes; Barry C Johnson; Kasthuraiah Maddali; Christophe Marchand; Yves Pommier
Activities, crystal structures, and molecular dynamics of dihydro-1H-isoindole derivatives, inhibitors of HIV-1 integrase.
Wei-Shau Hu; Stephen H Hughes
HIV-1 reverse transcription.
Paul L Boyer; Patrick K Clark; Stephen H Hughes
HIV-1 and HIV-2 reverse transcriptases: different mechanisms of resistance to nucleoside reverse transcriptase inhibitors.

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2013: Mathieu Métifiot; Steven J Smith; Xue Zhi Zhao; Terrence R Burke; Peter Cherepanov; Stephen Hare; Stephen H Hughes; Barry C Johnson; Kasthuraiah Maddali; Christophe Marchand; Yves Pommier
Activities, crystal structures, and molecular dynamics of dihydro-1H-isoindole derivatives, inhibitors of HIV-1 integrase.
ACS chemical biology 2013;8(1):209-17.
2012: Wei-Shau Hu; Stephen H Hughes
HIV-1 reverse transcription.
Cold Spring Harbor perspectives in medicine 2012;2(10):.
2012: Paul L Boyer; Patrick K Clark; Stephen H Hughes
HIV-1 and HIV-2 reverse transcriptases: different mechanisms of resistance to nucleoside reverse transcriptase inhibitors.
Journal of virology 2012;86(10):5885-94.
2012: Zandrea Ambrose; Alan Engelman; Stephen H Hughes; KyeongEun Lee; James Matous; Jean Ndjomou; Ilker Oztop; Taichiro Takemura; Derya Unutmaz; Hongzhan Xu; Vineet N KewalRamani
Human immunodeficiency virus type 1 capsid mutation N74D alters cyclophilin A dependence and impairs macrophage infection.
Journal of virology 2012;86(8):4708-14.
2012: Suhman Chung; Stephen H Hughes; Barry C Johnson; Jennifer T Miller; Stuart F J Le Grice
Mutagenesis of human immunodeficiency virus reverse transcriptase p51 subunit defines residues contributing to vinylogous urea inhibition of ribonuclease H activity.
The Journal of biological chemistry 2012;287(6):4066-75.
2012: Xue Zhi Zhao; Stephen H Hughes; Kasthuraiah Maddali; Christophe Marchand; Mathieu Metifiot; Yves Pommier; Steven J Smith; B Christie Vu; Terrence R Burke
Bicyclic hydroxy-1H-pyrrolopyridine-trione containing HIV-1 integrase inhibitors.
Chemical biology & drug design 2012;79(2):157-65.
2012: Barry C Johnson; David J Maloney; Disha Patel; Gary T Pauly; Ganesha Rai; Joel P Schneider; Craig J Thomas; Eddy Arnold; Heather L Baker; Joseph D Bauman; Kalyan Das; Stephen H Hughes
A comparison of the ability of rilpivirine (TMC278) and selected analogues to inhibit clinically relevant HIV-1 reverse transcriptase mutants.
Retrovirology 2012;9():99.
2011: Caroline A Davis; Michael A Parniak; Stephen H Hughes
The effects of RNase H inhibitors and nevirapine on the susceptibility of HIV-1 to AZT and 3TC.
Virology 2011;419(2):64-71.
2011: Stanislaw J Kaczmarczyk; Kalavathy Sitaraman; Howard A Young; Stephen H Hughes; Deb K Chatterjee
Protein delivery using engineered virus-like particles.
Proceedings of the National Academy of Sciences of the United States of America 2011;108(41):16998-7003.
2011: Xue Zhi Zhao; Kasthuraiah Maddali; Mathieu Metifiot; Steven J Smith; B Christie Vu; Christophe Marchand; Stephen H Hughes; Yves Pommier; Terrence R Burke
Development of tricyclic hydroxy-1H-pyrrolopyridine-trione containing HIV-1 integrase inhibitors.
Bioorganic & medicinal chemistry letters 2011;21(10):2986-90.
2011: B Christie Vu; Paul L Boyer; Maqbool A Siddiqui; Victor E Marquez; Stephen H Hughes
4'-C-methyl-2'-deoxyadenosine and 4'-C-ethyl-2'-deoxyadenosine inhibit HIV-1 replication.
Antimicrobial agents and chemotherapy 2011;55(5):2379-89.
2011: Eun-Gyung Lee; Jacqueline Roy; Dana Jackson; Patrick Clark; Paul L Boyer; Stephen H Hughes; Maxine L Linial
Foamy retrovirus integrase contains a Pol dimerization domain required for protease activation.
Journal of virology 2011;85(4):1655-61.
2010: Xiongying Tu; Kalyan Das; Qianwei Han; Joseph D Bauman; Arthur D Clark; Xiaorong Hou; Yulia V Frenkel; Barbara L Gaffney; Roger A Jones; Paul L Boyer; Stephen H Hughes; Stefan G Sarafianos; Eddy Arnold
Structural basis of HIV-1 resistance to AZT by excision.
Nature structural & molecular biology 2010;17(10):1202-9.
2010: Michael E Abram; Andrea L Ferris; Wei Shao; W Gregory Alvord; Stephen H Hughes
Nature, position, and frequency of mutations made in a single cycle of HIV-1 replication.
Journal of virology 2010;84(19):9864-78.
2010: KyeongEun Lee; Zandrea Ambrose; Thomas D Martin; Ilker Oztop; Alok Mulky; John G Julias; Nick Vandegraaff; Joerg G Baumann; Rui Wang; Wendy Yuen; Taichiro Takemura; Kenneth Shelton; Ichiro Taniuchi; Yuan Li; Joseph Sodroski; Dan R Littman; John M Coffin; Stephen H Hughes; Derya Unutmaz; Alan Engelman; Vineet N KewalRamani
Flexible use of nuclear import pathways by HIV-1.
Cell host & microbe 2010;7(3):221-33.
2010: Andrea L Ferris; Xiaolin Wu; Christina M Hughes; Claudia Stewart; Steven J Smith; Thomas A Milne; Gang G Wang; Ming-Chieh Shun; C David Allis; Alan Engelman; Stephen H Hughes
Lens epithelium-derived growth factor fusion proteins redirect HIV-1 DNA integration.
Proceedings of the National Academy of Sciences of the United States of America 2010;107(7):3135-40.
2009: Daniel M Himmel; Karen A Maegley; Tom A Pauly; Joseph D Bauman; Kalyan Das; Chhaya Dharia; Arthur D Clark; Kevin Ryan; Michael J Hickey; Robert A Love; Stephen H Hughes; Simon Bergqvist; Eddy Arnold
Structure of HIV-1 reverse transcriptase with the inhibitor beta-Thujaplicinol bound at the RNase H active site.
Structure (London, England : 1993) 2009;17(12):1625-35.
2009: Linda L Dunn; Mary Jane McWilliams; Kalyan Das; Eddy Arnold; Stephen H Hughes
Mutations in the thumb allow human immunodeficiency virus type 1 reverse transcriptase to be cleaved by protease in virions.
Journal of virology 2009;83(23):12336-44.
2009: Paul L Boyer; B Christie Vu; Zandrea Ambrose; John G Julias; Svenja Warnecke; Chenzhong Liao; Chris Meier; Victor E Marquez; Stephen H Hughes
The nucleoside analogue D-carba T blocks HIV-1 reverse transcription.
Journal of medicinal chemistry 2009;52(17):5356-64.
2009: Pamela L Russ; Maria J Gonzalez-Moa; B Christie Vu; Dina M Sigano; James A Kelley; Christopher C Lai; Jeffrey Deschamps; Stephen H Hughes; Victor E Marquez
North- and south-bicyclo[3.1.0]hexene nucleosides: the effect of ring planarity on anti-HIV activity.
ChemMedChem 2009;4(8):1354-63.
2009: Xue Zhi Zhao; Kasthuraiah Maddali; B Christie Vu; Christophe Marchand; Stephen H Hughes; Yves Pommier; Terrence R Burke
Examination of halogen substituent effects on HIV-1 integrase inhibitors derived from 2,3-dihydro-6,7-dihydroxy-1H-isoindol-1-ones and 4,5-dihydroxy-1H-isoindole-1,3(2H)-diones.
Bioorganic & medicinal chemistry letters 2009;19(10):2714-7.
2008: Jangsuk Oh; Kevin W Chang; Stephen H Hughes
Integration of rous sarcoma virus DNA: a CA dinucleotide is not required for integration of the U3 end of viral DNA.
Journal of virology 2008;82(22):11480-3.
2008: Joseph D Bauman; Kalyan Das; William C Ho; Mukta Baweja; Daniel M Himmel; Arthur D Clark; Deena A Oren; Paul L Boyer; Stephen H Hughes; Aaron J Shatkin; Eddy Arnold
Crystal engineering of HIV-1 reverse transcriptase for structure-based drug design.
Nucleic acids research 2008;36(15):5083-92.
2008: Kevin W Chang; Jangsuk Oh; W Gregory Alvord; Stephen H Hughes
The effects of alternate polypurine tracts (PPTs) and mutations of sequences adjacent to the PPT on viral replication and cleavage specificity of the Rous sarcoma virus reverse transcriptase.
Journal of virology 2008;82(17):8592-604.
2008: Krista A Delviks-Frankenberry; Galina N Nikolenko; Paul L Boyer; Stephen H Hughes; John M Coffin; Abhay Jere; Vinay K Pathak
HIV-1 reverse transcriptase connection subdomain mutations reduce template RNA degradation and enhance AZT excision.
Proceedings of the National Academy of Sciences of the United States of America 2008;105(31):10943-8.
2008: Maria J Comin; B Christie Vu; Paul L Boyer; Chenzhong Liao; Stephen H Hughes; Victor E Marquez
D-(+)-iso-methanocarbathymidine: a high-affinity substrate for herpes simplex virus 1 thymidine kinase.
ChemMedChem 2008;3(7):1129-34.
2008: Mary Jane McWilliams; John G Julias; Stephen H Hughes
Mutations in the human immunodeficiency virus type 1 polypurine tract (PPT) reduce the rate of PPT cleavage and plus-strand DNA synthesis.
Journal of virology 2008;82(10):5104-8.
2008: Kalyan Das; Joseph D Bauman; Arthur D Clark; Yulia V Frenkel; Paul J Lewi; Aaron J Shatkin; Stephen H Hughes; Eddy Arnold
High-resolution structures of HIV-1 reverse transcriptase/TMC278 complexes: strategic flexibility explains potency against resistance mutations.
Proceedings of the National Academy of Sciences of the United States of America 2008;105(5):1466-71.
2008: Hisao Saneyoshi; B Christie Vu; Stephen H Hughes; Paul L Boyer; Stefan G Sarafianos; Victor E Marquez
Synthesis of conformationally locked carbocyclic nucleoside phosphonates to probe the active site of HIV-1 RT.
Nucleic acids symposium series (2004) 2008;(52):623-4.
2008: Jangsuk Oh; Mary Jane McWilliams; John G Julias; Stephen H Hughes
Mutations in the U5 region adjacent to the primer binding site affect tRNA cleavage by human immunodeficiency virus type 1 reverse transcriptase in vivo.
Journal of virology 2008;82(2):719-27.
2008: Jangsuk Oh; Kevin W Chang; Rafal Wierzchoslawski; W Gregory Alvord; Stephen H Hughes
Rous sarcoma virus (RSV) integration in vivo: a CA dinucleotide is not required in U3, and RSV linear DNA does not autointegrate.
Journal of virology 2008;82(1):503-12.
2007: chia-kuei wu; Karine Gousset; Stephen H Hughes
Targeting to the endoplasmic reticulum improves the folding of recombinant human telomerase reverse transcriptase.
Protein expression and purification 2007;56(1):8-19.
2007: Paul L Boyer; Carolyn R Stenbak; David Hoberman; Maxine L Linial; Stephen H Hughes
In vitro fidelity of the prototype primate foamy virus (PFV) RT compared to HIV-1 RT.
Virology 2007;367(2):253-64.
2007: Paul L Boyer; John G Julias; Zandrea Ambrose; Maqbool A Siddiqui; Victor E Marquez; Stephen H Hughes
The nucleoside analogs 4'C-methyl thymidine and 4'C-ethyl thymidine block DNA synthesis by wild-type HIV-1 RT and excision proficient NRTI resistant RT variants.
Journal of molecular biology 2007;371(4):873-82.
2007: Marshall L Morningstar; Thomas Roth; David W Farnsworth; Marilyn Kroeger Smith; Karen Watson; Robert W Buckheit; Kalyan Das; Wanyi Zhang; Eddy Arnold; John G Julias; Stephen H Hughes; Christopher J Michejda
Synthesis, biological activity, and crystal structure of potent nonnucleoside inhibitors of HIV-1 reverse transcriptase that retain activity against mutant forms of the enzyme.
Journal of medicinal chemistry 2007;50(17):4003-15.
2007: David Derse; Bruce J Crise; Yuan Li; Gerald Princler; Nicole Lum; Claudia Stewart; Connor F McGrath; Stephen H Hughes; David J Munroe; Xiaolin Wu
Human T-cell leukemia virus type 1 integration target sites in the human genome: comparison with those of other retroviruses.
Journal of virology 2007;81(12):6731-41.
2007: Kalyan Das; Stefan G Sarafianos; Arthur D Clark; Paul L Boyer; Stephen H Hughes; Eddy Arnold
Crystal structures of clinically relevant Lys103Asn/Tyr181Cys double mutant HIV-1 reverse transcriptase in complexes with ATP and non-nucleoside inhibitor HBY 097.
Journal of molecular biology 2007;365(1):77-89.
2006: Daniel M Himmel; Stefan G Sarafianos; Sanjeewa Dharmasena; Mohammed M Hossain; Kessler McCoy-Simandle; Tatiana Ilina; Arthur D Clark; Jennifer L Knight; John G Julias; Patrick K Clark; Karsten Krogh-Jespersen; Ronald M Levy; Stephen H Hughes; Michael A Parniak; Eddy Arnold
HIV-1 reverse transcriptase structure with RNase H inhibitor dihydroxy benzoyl naphthyl hydrazone bound at a novel site.
ACS chemical biology 2006;1(11):702-12.
2006: Jangsuk Oh; Kevin W Chang; W Gregory Alvord; Stephen H Hughes
Alternate polypurine tracts affect rous sarcoma virus integration in vivo.
Journal of virology 2006;80(20):10281-4.
2006: Victor E Marquez; Stephen H Hughes; Shizuko Sei; Riad Agbaria
The history of N-methanocarbathymidine: the investigation of a conformational concept leads to the discovery of a potent and selective nucleoside antiviral agent.
Antiviral research 2006;71(2-3):268-75.
2006: Mary Jane McWilliams; John G Julias; Stefan G Sarafianos; W Gregory Alvord; Eddy Arnold; Stephen H Hughes
Combining mutations in HIV-1 reverse transcriptase with mutations in the HIV-1 polypurine tract affects RNase H cleavages involved in PPT utilization.
Virology 2006;348(2):378-88.
2006: Zandrea Ambrose; John G Julias; Paul L Boyer; Vineet N KewalRamani; Stephen H Hughes
The level of reverse transcriptase (RT) in human immunodeficiency virus type 1 particles affects susceptibility to nonnucleoside RT inhibitors but not to lamivudine.
Journal of virology 2006;80(5):2578-81.
2006: Paul L Boyer; Stefan G Sarafianos; Patrick K Clark; Eddy Arnold; Stephen H Hughes
Why do HIV-1 and HIV-2 use different pathways to develop AZT resistance?
PLoS pathogens 2006;2(2):e10.
2006: Jangsuk Oh; Kevin W Chang; Stephen H Hughes
Mutations in the U5 sequences adjacent to the primer binding site do not affect tRNA cleavage by rous sarcoma virus RNase H but do cause aberrant integrations in vivo.
Journal of virology 2006;80(1):451-9.
2005: Daniel M Himmel; Kalyan Das; Arthur D Clark; Stephen H Hughes; Abdellah Benjahad; Said Oumouch; Jérôme Guillemont; Sophie Coupa; Alain PONCELET; Imre Csoka; Christophe Meyer; Koen Andries; Chi Hung Nguyen; David S Grierson; Eddy Arnold
Crystal structures for HIV-1 reverse transcriptase in complexes with three pyridinone derivatives: a new class of non-nucleoside inhibitors effective against a broad range of drug-resistant strains.
Journal of medicinal chemistry 2005;48(24):7582-91.
2005: Kevin W Chang; John G Julias; W Gregory Alvord; Jangsuk Oh; Stephen H Hughes
Alternate polypurine tracts (PPTs) affect the rous sarcoma virus RNase H cleavage specificity and reveal a preferential cleavage following a GA dinucleotide sequence at the PPT-U3 junction.
Journal of virology 2005;79(21):13694-704.
2005: Kevin W Chang; Eugene V Barsov; Andrea L Ferris; Stephen H Hughes
Mutations of a residue within the polyproline-rich region of Env alter the replication rate and level of cytopathic effects in chimeric avian retroviral vectors.
Journal of virology 2005;79(16):10258-67.
2005: Paul A J Janssen; Paul J Lewi; Eddy Arnold; Frits Daeyaert; Marc de Jonge; Jan Heeres; Lucien M H Koymans; Maarten Vinkers; Jérôme Guillemont; Elisabeth Pasquier; Michael J Kukla; Donald W Ludovici; Koen Andries; Marie-Pierre de Bethune; Rudi Pauwels; Kalyan Das; Arthur D Clark; Yulia Volovik Frenkel; Stephen H Hughes; Bart Medaer; Fons De Knaep; Hilde Bohets; Fred De Clerck; Ann Lampo; Peter Williams; Paul Stoffels
In search of a novel anti-HIV drug: multidisciplinary coordination in the discovery of 4-[[4-[[4-[(1E)-2-cyanoethenyl]-2,6-dimethylphenyl]amino]-2- pyrimidinyl]amino]benzonitrile (R278474, rilpivirine).
Journal of medicinal chemistry 2005;48(6):1901-9.
2005: Paul L Boyer; John G Julias; Victor E Marquez; Stephen H Hughes
Fixed conformation nucleoside analogs effectively inhibit excision-proficient HIV-1 reverse transcriptases.
Journal of molecular biology 2005;345(3):441-50.
2004: Stefan G Sarafianos; Kalyan Das; Stephen H Hughes; Eddy Arnold
Taking aim at a moving target: designing drugs to inhibit drug-resistant HIV-1 reverse transcriptases.
Current opinion in structural biology 2004;14(6):716-30.
2004: Zandrea Ambrose; Valerie F Boltz; Sarah Palmer; John M Coffin; Stephen H Hughes; Vineet N KewalRamani
In vitro characterization of a simian immunodeficiency virus-human immunodeficiency virus (HIV) chimera expressing HIV type 1 reverse transcriptase to study antiviral resistance in pigtail macaques.
Journal of virology 2004;78(24):13553-61.
2004: John G Julias; Mary Jane McWilliams; Stefan G Sarafianos; W Gregory Alvord; Eddy Arnold; Stephen H Hughes
Effects of mutations in the G tract of the human immunodeficiency virus type 1 polypurine tract on virus replication and RNase H cleavage.
Journal of virology 2004;78(23):13315-24.
2004: Paul L Boyer; Tomozumi Imamichi; Stefan G Sarafianos; Eddy Arnold; Stephen H Hughes
Effects of the Delta67 complex of mutations in human immunodeficiency virus type 1 reverse transcriptase on nucleoside analog excision.
Journal of virology 2004;78(18):9987-97.
2004: Stefan G Sarafianos; Stephen H Hughes; Eddy Arnold
Designing anti-AIDS drugs targeting the major mechanism of HIV-1 RT resistance to nucleoside analog drugs.
The international journal of biochemistry & cell biology 2004;36(9):1706-15.
2004: Mamuka Kvaratskhelia; Patrick K Clark; Sonja Hess; Deborah C Melder; Mark J Federspiel; Stephen H Hughes
Identification of glycosylation sites in the SU component of the Avian Sarcoma/Leukosis virus Envelope Glycoprotein (Subgroup A) by mass spectrometry.
Virology 2004;326(1):171-81.
2004: Paul L Boyer; Carolyn R Stenbak; Patrick K Clark; Maxine L Linial; Stephen H Hughes
Characterization of the polymerase and RNase H activities of human foamy virus reverse transcriptase.
Journal of virology 2004;78(12):6112-21.
2004: Kalyan Das; Arthur D Clark; Paul J Lewi; Jan Heeres; Marc R. de Jonge; Lucien M H Koymans; H Maarten Vinkers; Frits Daeyaert; Donald W Ludovici; Michael J Kukla; Bart De Corte; Robert W Kavash; Chih Y Ho; Hong Ye; Mark A Lichtenstein; Koen Andries; Rudi Pauwels; Marie-Pierre de Bethune; Paul L Boyer; Patrick Clark; Stephen H Hughes; Paul A J Janssen; Eddy Arnold
Roles of conformational and positional adaptability in structure-based design of TMC125-R165335 (etravirine) and related non-nucleoside reverse transcriptase inhibitors that are highly potent and effective against wild-type and drug-resistant HIV-1 variants.
Journal of medicinal chemistry 2004;47(10):2550-60.
2004: Steve Tuske; Stefan G Sarafianos; Arthur D Clark; Jianping Ding; Lisa K Naeger; Kirsten L White; Michael D Miller; Craig S Gibbs; Paul L Boyer; Patrick Clark; Gang Wang; Barbara L Gaffney; Roger A Jones; Donald M Jerina; Stephen H Hughes; Eddy Arnold
Structures of HIV-1 RT-DNA complexes before and after incorporation of the anti-AIDS drug tenofovir.
Nature structural & molecular biology 2004;11(5):469-74.
2004: John G Julias; Paul L Boyer; Mary Jane McWilliams; W Gregory Alvord; Stephen H Hughes
Mutations at position 184 of human immunodeficiency virus type-1 reverse transcriptase affect virus titer and viral DNA synthesis.
Virology 2004;322(1):13-21.
2004: Elena N Peletskaya; Alex A Kogon; Steve Tuske; Eddy Arnold; Stephen H Hughes
Nonnucleoside inhibitor binding affects the interactions of the fingers subdomain of human immunodeficiency virus type 1 reverse transcriptase with DNA.
Journal of virology 2004;78(7):3387-97.
2004: Stephen H Hughes
The RCAS vector system.
Folia biologica 2004;50(3-4):107-19.
2003: Gerald Princler; John G Julias; Stephen H Hughes; David Derse
Roles of viral and cellular proteins in the expression of alternatively spliced HTLV-1 pX mRNAs.
Virology 2003;317(1):136-45.
2003: Mary Jane McWilliams; John G Julias; Stefan G Sarafianos; W Gregory Alvord; Eddy Arnold; Stephen H Hughes
Mutations in the 5' end of the human immunodeficiency virus type 1 polypurine tract affect RNase H cleavage specificity and virus titer.
Journal of virology 2003;77(20):11150-7.
2003: John G Julias; Mary Jane McWilliams; Stefan G Sarafianos; W Gregory Alvord; Eddy Arnold; Stephen H Hughes
Mutation of amino acids in the connection domain of human immunodeficiency virus type 1 reverse transcriptase that contact the template-primer affects RNase H activity.
Journal of virology 2003;77(15):8548-54.
2003: Marie-Pierre Golinelli; Stephen H Hughes
Secondary structure in the nucleic acid affects the rate of HIV-1 nucleocapsid-mediated strand annealing.
Biochemistry 2003;42(27):8153-62.
2003: Marilyn B Kroeger Smith; Brian M Hose; Arie Hawkins; James Lipchock; David W Farnsworth; Robert C Rizzo; Julian Tirado-Rives; Eddy Arnold; Wanyi Zhang; Stephen H Hughes; William L Jorgensen; Christopher J Michejda; Richard H Smith
Molecular modeling calculations of HIV-1 reverse transcriptase nonnucleoside inhibitors: correlation of binding energy with biological activity for novel 2-aryl-substituted benzimidazole analogues.
Journal of medicinal chemistry 2003;46(10):1940-7.
2003: Stefan G Sarafianos; Arthur D Clark; Steve Tuske; Christopher J Squire; Kalyan Das; Dequan Sheng; Palanichamy Ilankumaran; Andagar R Ramesha; Heiko Kroth; Jane M Sayer; Donald M Jerina; Paul L Boyer; Stephen H Hughes; Eddy Arnold
Trapping HIV-1 reverse transcriptase before and after translocation on DNA.
The Journal of biological chemistry 2003;278(18):16280-8.
2003: Paul J Lewi; Marc de Jonge; Frits Daeyaert; Lucien M H Koymans; Maarten Vinkers; Jan Heeres; Paul A J Janssen; Eddy Arnold; Kalyan Das; Arthur D Clark; Stephen H Hughes; Paul L Boyer; Marie-Pierre de Bethune; Rudi Pauwels; Koen Andries; Michael J Kukla; Donald W Ludovici; Bart De Corte; Robert W Kavash; Chih Ho; Paul J Lewis
On the detection of multiple-binding modes of ligands to proteins, from biological, structural, and modeling data.
Journal of computer-aided molecular design 2003;17(2-4):129-34.
2003: Marilyn B Kroeger Smith; Sandra Ruby; Stanislav Horouzhenko; Bryan Buckingham; Julia Richardson; Ina O'Carroll; Emily Potts; William L Jorgensen; Eddy Arnold; Wanyi Zhang; Stephen H Hughes; Christopher J Michejda; Richard H Smith
HIV-1 reverse transcriptase variants: molecular modeling of Y181C, V106A, L100I, and K103N mutations with nonnucleoside inhibitors using Monte Carlo simulations in combination with a linear response method.
Drug design and discovery 2003;18(4):151-63.
2002: Stefan G Sarafianos; Arthur D Clark; Kalyan Das; Steve Tuske; Jens J Birktoft; Palanichamy Ilankumaran; Andagar R Ramesha; Jane M Sayer; Donald M Jerina; Paul L Boyer; Stephen H Hughes; Eddy Arnold
Structures of HIV-1 reverse transcriptase with pre- and post-translocation AZTMP-terminated DNA.
The EMBO journal 2002;21(23):6614-24.
2002: A Geoffrey Skillman; Karl W Maurer; Diana C Roe; Margaret J Stauber; Dolan Eargle; Todd J A Ewing; Angelika Muscate; Elisabeth Davioud; Maxine V Medaglia; Robert J Fisher; Eddy Arnold; Hong Qiang Gao; Robert W Buckheit; Paul L Boyer; Stephen H Hughes; Irwin D Kuntz; George L Kenyon
A novel mechanism for inhibition of HIV-1 reverse transcriptase.
Bioorganic chemistry 2002;30(6):443-58.
2002: Paul L Boyer; Stefan G Sarafianos; Eddy Arnold; Stephen H Hughes
Nucleoside analog resistance caused by insertions in the fingers of human immunodeficiency virus type 1 reverse transcriptase involves ATP-mediated excision.
Journal of virology 2002;76(18):9143-51.
2002: John G Julias; Mary Jane McWilliams; Stefan G Sarafianos; Eddy Arnold; Stephen H Hughes
Mutations in the RNase H domain of HIV-1 reverse transcriptase affect the initiation of DNA synthesis and the specificity of RNase H cleavage in vivo.
Proceedings of the National Academy of Sciences of the United States of America 2002;99(14):9515-20.
2002: Marie-Pierre Golinelli; Stephen H Hughes
Nontemplated nucleotide addition by HIV-1 reverse transcriptase.
Biochemistry 2002;41(18):5894-906.
2002: Jason W Rausch; Daniela Lener; Jennifer T Miller; John G Julias; Stephen H Hughes; Stuart F J Le Grice
Altering the RNase H primer grip of human immunodeficiency virus reverse transcriptase modifies cleavage specificity.
Biochemistry 2002;41(15):4856-65.
2002: Paul L Boyer; Stefan G Sarafianos; Eddy Arnold; Stephen H Hughes
The M184V mutation reduces the selective excision of zidovudine 5'-monophosphate (AZTMP) by the reverse transcriptase of human immunodeficiency virus type 1.
Journal of virology 2002;76(7):3248-56.
2002: Marie-Pierre Golinelli; Stephen H Hughes
Nontemplated base addition by HIV-1 RT can induce nonspecific strand transfer in vitro.
Virology 2002;294(1):122-34.
2002: Jangsuk Oh; John G Julias; Andrea L Ferris; Stephen H Hughes
Construction and characterization of a replication-competent retroviral shuttle vector plasmid.
Journal of virology 2002;76(4):1762-8.
2001: Hong Qiang Gao; Stefan G Sarafianos; Eddy Arnold; Stephen H Hughes
RNase H cleavage of the 5' end of the human immunodeficiency virus type 1 genome.
Journal of virology 2001;75(23):11874-80.
2001: Elena N Peletskaya; Paul L Boyer; A A Kogon; P Clark; Heiko Kroth; Jane M Sayer; Donald M Jerina; Stephen H Hughes
Cross-linking of the fingers subdomain of human immunodeficiency virus type 1 reverse transcriptase to template-primer.
Journal of virology 2001;75(19):9435-45.
2001: Ziv Sevilya; Shoshana Loya; Stephen H Hughes; Amnon Hizi
The ribonuclease H activity of the reverse transcriptases of human immunodeficiency viruses type 1 and type 2 is affected by the thumb subdomain of the small protein subunits.
Journal of molecular biology 2001;311(5):957-71.
2001: Marie-Pierre Golinelli; Stephen H Hughes
Self-priming of retroviral minus-strand strong-stop DNAs.
Virology 2001;285(2):278-90.
2001: John G Julias; A L Ferris; Paul L Boyer; Stephen H Hughes
Replication of phenotypically mixed human immunodeficiency virus type 1 virions containing catalytically active and catalytically inactive reverse transcriptase.
Journal of virology 2001;75(14):6537-46.
2001: Paul L Boyer; Hong Qiang Gao; Patrick K Clark; Stefan G Sarafianos; Eddy Arnold; Stephen H Hughes
YADD mutants of human immunodeficiency virus type 1 and Moloney murine leukemia virus reverse transcriptase are resistant to lamivudine triphosphate (3TCTP) in vitro.
Journal of virology 2001;75(14):6321-8.
2001: Y Hsiou; Jianping Ding; Kalyan Das; Arthur D Clark; Paul L Boyer; Paul J Lewi; Paul A J Janssen; JP Kleim; M Rösner; Stephen H Hughes; Eddy Arnold
The Lys103Asn mutation of HIV-1 RT: a novel mechanism of drug resistance.
Journal of molecular biology 2001;309(2):437-45.
2001: Eugene V Barsov; William Samuel Payne; Stephen H Hughes
Adaptation of chimeric retroviruses in vitro and in vivo: isolation of avian retroviral vectors with extended host range.
Journal of virology 2001;75(11):4973-83.
2001: Paul L Boyer; Hong Qiang Gao; P Frank; Patrick K Clark; Stephen H Hughes
The basic loop of the RNase H domain of MLV RT is important both for RNase H and for polymerase activity.
Virology 2001;282(1):206-13.
2001: Stefan G Sarafianos; Kalyan Das; C Tantillo; Arthur D Clark; Jianping Ding; J M Whitcomb; Paul L Boyer; Stephen H Hughes; Eddy Arnold
Crystal structure of HIV-1 reverse transcriptase in complex with a polypurine tract RNA:DNA.
The EMBO journal 2001;20(6):1449-61.
2001: M D Driscoll; M P Golinelli; Stephen H Hughes
In vitro analysis of human immunodeficiency virus type 1 minus-strand strong-stop DNA synthesis and genomic RNA processing.
Journal of virology 2001;75(2):672-86.
2000: V B Pinto; S Prasad; jon yewdell; Jack R Bennink; Stephen H Hughes
Restricting expression prolongs expression of foreign genes introduced into animals by retroviruses.
Journal of virology 2000;74(21):10202-6.
2000: M D Driscoll; Stephen H Hughes
Human immunodeficiency virus type 1 nucleocapsid protein can prevent self-priming of minus-strand strong stop DNA by promoting the annealing of short oligonucleotides to hairpin sequences.
Journal of virology 2000;74(19):8785-92.
2000: Hong Qiang Gao; Paul L Boyer; Stefan G Sarafianos; Eddy Arnold; Stephen H Hughes
The role of steric hindrance in 3TC resistance of human immunodeficiency virus type-1 reverse transcriptase.
Journal of molecular biology 2000;300(2):403-18.
2000: Paul L Boyer; Stephen H Hughes
Effects of amino acid substitutions at position 115 on the fidelity of human immunodeficiency virus type 1 reverse transcriptase.
Journal of virology 2000;74(14):6494-500.
1999: Hong Qiang Gao; Stefan G Sarafianos; Eddy Arnold; Stephen H Hughes
Similarities and differences in the RNase H activities of human immunodeficiency virus type 1 reverse transcriptase and Moloney murine leukemia virus reverse transcriptase.
Journal of molecular biology 1999;294(5):1097-113.
1999: Sheri L Holmen; DW Salter; William Samuel Payne; Jerry B Dodgson; Stephen H Hughes; Mark J Federspiel
Soluble forms of the subgroup A avian leukosis virus [ALV(A)] receptor Tva significantly inhibit ALV(A) infection in vitro and in vivo.
Journal of virology 1999;73(12):10051-60.
1999: B A Ortiz-Conde; Stephen H Hughes
Studies of the genomic RNA of leukosis viruses: implications for RNA dimerization.
Journal of virology 1999;73(9):7165-74.
1999: Stefan G Sarafianos; Kalyan Das; Arthur D Clark; Jianping Ding; Paul L Boyer; Stephen H Hughes; Eddy Arnold
Lamivudine (3TC) resistance in HIV-1 reverse transcriptase involves steric hindrance with beta-branched amino acids.
Proceedings of the National Academy of Sciences of the United States of America 1999;96(18):10027-32.
1999: X H Zheng; Stephen H Hughes
An avian sarcoma/leukosis virus-based gene trap vector for mammalian cells.
Journal of virology 1999;73(8):6946-52.
1999: Stefan G Sarafianos; Kalyan Das; Jianping Ding; Paul L Boyer; Stephen H Hughes; Eddy Arnold
Touching the heart of HIV-1 drug resistance: the fingers close down on the dNTP at the polymerase active site.
Chemistry & biology 1999;6(5):R137-46.
1999: Paul L Boyer; Julianna Lisziewicz; F Lori; Stephen H Hughes
Analysis of amino insertion mutations in the fingers subdomain of HIV-1 reverse transcriptase.
Journal of molecular biology 1999;286(4):995-1008.
1998: Jianping Ding; Kalyan Das; Y Hsiou; Stefan G Sarafianos; Arthur D Clark; A Jacobo-Molina; C Tantillo; Stephen H Hughes; Eddy Arnold
Structure and functional implications of the polymerase active site region in a complex of HIV-1 RT with a double-stranded DNA template-primer and an antibody Fab fragment at 2.8 A resolution.
Journal of molecular biology 1998;284(4):1095-111.
1998: Y Hsiou; Kalyan Das; Jianping Ding; Arthur D Clark; JP Kleim; M Rösner; I Winkler; G Riess; Stephen H Hughes; Eddy Arnold
Structures of Tyr188Leu mutant and wild-type HIV-1 reverse transcriptase complexed with the non-nucleoside inhibitor HBY 097: inhibitor flexibility is a useful design feature for reducing drug resistance.
Journal of molecular biology 1998;284(2):313-23.
1998: Janet L Schaefer-Klein; I Givol; Eugene V Barsov; J M Whitcomb; M VanBrocklin; D N Foster; Mark J Federspiel; Stephen H Hughes
The EV-O-derived cell line DF-1 supports the efficient replication of avian leukosis-sarcoma viruses and vectors.
Virology 1998;248(2):305-11.
1998: I Givol; D Givol; Stephen H Hughes
Overexpression of p21waf1/cip1 arrests the growth of chicken embryo fibroblasts that overexpress E2F1.
Oncogene 1998;16(24):3115-22.
1998: Hong Qiang Gao; Paul L Boyer; Eddy Arnold; Stephen H Hughes
Effects of mutations in the polymerase domain on the polymerase, RNase H and strand transfer activities of human immunodeficiency virus type 1 reverse transcriptase.
Journal of molecular biology 1998;277(3):559-72.
1998: Paul L Boyer; Hong Qiang Gao; Stephen H Hughes
A mutation at position 190 of human immunodeficiency virus type 1 reverse transcriptase interacts with mutations at positions 74 and 75 via the template primer.
Antimicrobial agents and chemotherapy 1998;42(2):447-52.
1997: Marilyn B Kroeger Smith; Christopher J Michejda; Stephen H Hughes; Paul L Boyer; Paul A J Janssen; Koen Andries; Robert W Buckheit; Richard H Smith
Molecular modeling of HIV-1 reverse transcriptase drug-resistant mutant strains: implications for the mechanism of polymerase action.
Protein engineering 1997;10(12):1379-83.
1997: Shoshana Loya; Hong Qiang Gao; Orna Avidan; Paul L Boyer; Stephen H Hughes; Amnon Hizi
Subunit-specific mutagenesis of the cysteine 280 residue of the reverse transcriptase of human immunodeficiency virus type 1: effects on sensitivity to a specific inhibitor of the RNase H activity.
Journal of virology 1997;71(7):5668-72.
1997: M Perach; T Rubinek; Stephen H Hughes; Amnon Hizi
Analysis of HIV-2 RT mutants provides evidence that resistance of HIV-1 RT and HIV-2 RT to nucleoside analogs involves a repositioning of the template-primer.
Journal of molecular biology 1997;268(3):648-54.
1997: M VanBrocklin; A L Ferris; Stephen H Hughes; Mark J Federspiel
Expression of a murine leukemia virus Gag-Escherichia coli RNase HI fusion polyprotein significantly inhibits virus spread.
Journal of virology 1997;71(4):3312-8.
1997: Jianping Ding; Stephen H Hughes; Eddy Arnold
Protein-nucleic acid interactions and DNA conformation in a complex of human immunodeficiency virus type 1 reverse transcriptase with a double-stranded DNA template-primer.
Biopolymers 1997;44(2):125-38.
1996: Kalyan Das; J Ding; Y Hsiou; Arthur D Clark; HE Moereels; L Koymans; Koen Andries; R Pauwels; Paul A J Janssen; Paul L Boyer; P Clark; Richard H Smith; Marilyn B Kroeger Smith; Christopher J Michejda; Stephen H Hughes; Eddy Arnold
Crystal structures of 8-Cl and 9-Cl TIBO complexed with wild-type HIV-1 RT and 8-Cl TIBO complexed with the Tyr181Cys HIV-1 RT drug-resistant mutant.
Journal of molecular biology 1996;264(5):1085-100.
1996: Y Hsiou; J Ding; Kalyan Das; Arthur D Clark; Stephen H Hughes; Eddy Arnold
Structure of unliganded HIV-1 reverse transcriptase at 2.7 A resolution: implications of conformational changes for polymerization and inhibition mechanisms.
Structure (London, England : 1993) 1996;4(7):853-60.
1996: E V Barsov; W E Huber; J Marcotrigiano; P K Clark; Arthur D Clark; Eddy Arnold; Stephen H Hughes
Inhibition of human immunodeficiency virus type 1 integrase by the Fab fragment of a specific monoclonal antibody suggests that different multimerization states are required for different enzymatic functions.
Journal of virology 1996;70(7):4484-94.
1996: Paul L Boyer; Stephen H Hughes
Nucleoside-analogue resistance involves the p66 subunit of HIV-1 RT.
Nature structural biology 1996;3(7):579-80.
1996: C J Petropoulos; I Givol; Stephen H Hughes
Comparative analysis of the structure and function of the chicken c-myc and v-myc genes: v-myc is a more potent inducer of cell proliferation and apoptosis than c-myc.
Oncogene 1996;12(12):2611-21.
1996: M J Federspiel; D A Swing; B Eagleson; S W Reid; Stephen H Hughes
Expression of transduced genes in mice generated by infecting blastocysts with avian leukosis virus-based retroviral vectors.
Proceedings of the National Academy of Sciences of the United States of America 1996;93(10):4931-6.
1996: Stephen H Hughes; Z Hostomsky; S F Le Grice; K Lentz; Eddy Arnold
What is the orientation of DNA polymerases on their templates?
Journal of virology 1996;70(5):2679-83.
1996: Eddy Arnold; Kalyan Das; J Ding; P N Yadav; Y Hsiou; Paul L Boyer; Stephen H Hughes
Targeting HIV reverse transcriptase for anti-AIDS drug design: structural and biological considerations for chemotherapeutic strategies.
Drug design and discovery 1996;13(3-4):29-47.
1996: Paul L Boyer; Stephen H Hughes
Site-directed mutagenic analysis of viral polymerases and related proteins.
Methods in enzymology 1996;275():538-55.
1996: D P Lana; J M Leferovich; A M Kelly; Stephen H Hughes
Selective expression of a ski transgene affects IIb fast muscles and skeletal structure.
Developmental dynamics : an official publication of the American Association of Anatomists 1996;205(1):13-23.
1995: I Givol; D Givol; S Rulong; James Resau; Ilan Tsarfaty; Stephen H Hughes
Overexpression of human p21waf1/cip1 arrests the growth of chicken embryo fibroblasts transformed by individual oncogenes.
Oncogene 1995;11(12):2609-18.
1995: Marilyn B Kroeger Smith; C A Rouzer; Lisa Anne Taneyhill; N A Smith; Stephen H Hughes; Paul L Boyer; Paul A J Janssen; HE Moereels; L Koymans; Eddy Arnold
Molecular modeling studies of HIV-1 reverse transcriptase nonnucleoside inhibitors: total energy of complexation as a predictor of drug placement and activity.
Protein science : a publication of the Protein Society 1995;4(10):2203-22.
1995: J M Whitcomb; B A Ortiz-Conde; Stephen H Hughes
Replication of avian leukosis viruses with mutations at the primer binding site: use of alternative tRNAs as primers.
Journal of virology 1995;69(10):6228-38.
1995: Paul L Boyer; Stephen H Hughes
Analysis of mutations at position 184 in reverse transcriptase of human immunodeficiency virus type 1.
Antimicrobial agents and chemotherapy 1995;39(7):1624-8.
1995: Robert W Buckheit; V Fliakas-Boltz; S Yeagy-Bargo; O Weislow; D L Mayers; Paul L Boyer; Stephen H Hughes; B C Pan; S H Chu; JP Bader
Resistance to 1-[(2-hydroxyethoxy)methyl]-6-(phenylthio)thymine derivatives is generated by mutations at multiple sites in the HIV-1 reverse transcriptase.
Virology 1995;210(1):186-93.
1995: J Ding; Kalyan Das; HE Moereels; L Koymans; Koen Andries; Paul A J Janssen; Stephen H Hughes; Eddy Arnold
Structure of HIV-1 RT/TIBO R 86183 complex reveals similarity in the binding of diverse nonnucleoside inhibitors.
Nature structural biology 1995;2(5):407-15.
1995: P H Patel; A Jacobo-Molina; J Ding; C Tantillo; Arthur D Clark; R Raag; R G Nanni; Stephen H Hughes; Eddy Arnold
Insights into DNA polymerization mechanisms from structure and function analysis of HIV-1 reverse transcriptase.
Biochemistry 1995;34(16):5351-63.
1995: I Givol; Paul L Boyer; Stephen H Hughes
Isolation and characterization of the chicken c-sno gene.
Gene 1995;156(2):271-6.
1995: M Ghosh; Kathryn Howard; Craig E Cameron; S J Benkovic; Stephen H Hughes; Stuart F J Le Grice
Truncating alpha-helix E' of p66 human immunodeficiency virus reverse transcriptase modulates RNase H function and impairs DNA strand transfer.
The Journal of biological chemistry 1995;270(13):7068-76.
1995: Arthur D Clark; A Jacobo-Molina; P Clark; Stephen H Hughes; Eddy Arnold
Crystallization of human immunodeficiency virus type 1 reverse transcriptase with and without nucleic acid substrates, inhibitors, and an antibody Fab fragment.
Methods in enzymology 1995;262():171-85.
1995: J M Leferovich; D P Lana; P Sutrave; Stephen H Hughes; A M Kelly
Regulation of c-ski transgene expression in developing and mature mice.
The Journal of neuroscience : the official journal of the Society for Neuroscience 1995;15(1 Pt 2):596-603.
1994: M J Federspiel; P Bates; J A Young; Harold Varmus; Stephen H Hughes
A system for tissue-specific gene targeting: transgenic mice susceptible to subgroup A avian leukosis virus-based retroviral vectors.
Proceedings of the National Academy of Sciences of the United States of America 1994;91(23):11241-5.
1994: Paul L Boyer; A L Ferris; P Clark; J Whitmer; P Frank; C Tantillo; Eddy Arnold; Stephen H Hughes
Mutational analysis of the fingers and palm subdomains of human immunodeficiency virus type-1 (HIV-1) reverse transcriptase.
Journal of molecular biology 1994;243(3):472-83.
1994: C Tantillo; J Ding; A Jacobo-Molina; R G Nanni; Paul L Boyer; Stephen H Hughes; R Pauwels; Koen Andries; Paul A J Janssen; Eddy Arnold
Locations of anti-AIDS drug binding sites and resistance mutations in the three-dimensional structure of HIV-1 reverse transcriptase. Implications for mechanisms of drug inhibition and resistance.
Journal of molecular biology 1994;243(3):369-87.
1994: P Sutrave; B K Shafer; Jeffrey N Strathern; Stephen H Hughes
Isolation, identification and characterization of the FUN12 gene of Saccharomyces cerevisiae.
Gene 1994;146(2):209-13.
1994: M J Federspiel; Stephen H Hughes
Effects of the gag region on genome stability: avian retroviral vectors that contain sequences from the Bryan strain of Rous sarcoma virus.
Virology 1994;203(2):211-20.
1994: Paul L Boyer; J Ding; Eddy Arnold; Stephen H Hughes
Subunit specificity of mutations that confer resistance to nonnucleoside inhibitors in human immunodeficiency virus type 1 reverse transcriptase.
Antimicrobial agents and chemotherapy 1994;38(9):1909-14.
1994: Shoshana Loya; M Bakhanashvili; R Tal; Stephen H Hughes; Paul L Boyer; Amnon Hizi
Enzymatic properties of two mutants of reverse transcriptase of human immunodeficiency virus type 1 (tyrosine 181-->isoleucine and tyrosine 188-->leucine), resistant to nonnucleoside inhibitors.
AIDS research and human retroviruses 1994;10(8):939-46.
1994: Paul L Boyer; C Tantillo; A Jacobo-Molina; R G Nanni; J Ding; Eddy Arnold; Stephen H Hughes
Sensitivity of wild-type human immunodeficiency virus type 1 reverse transcriptase to dideoxynucleotides depends on template length; the sensitivity of drug-resistant mutants does not.
Proceedings of the National Academy of Sciences of the United States of America 1994;91(11):4882-6.
1994: I Givol; Ilan Tsarfaty; James Resau; S Rulong; P P da Silva; G Nasioulas; James B DuHadaway; Stephen H Hughes; Donald l Ewert
Bcl-2 expressed using a retroviral vector is localized primarily in the nuclear membrane and the endoplasmic reticulum of chicken embryo fibroblasts.
Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research 1994;5(4):419-29.
1994: T Rubinek; Shoshana Loya; M Shaharabany; Stephen H Hughes; P K Clark; Amnon Hizi
The catalytic properties of the reverse transcriptase of the lentivirus equine infectious anemia virus.
European journal of biochemistry / FEBS 1994;219(3):977-83.
1993: J M Whitcomb; A Rashtchian; Stephen H Hughes
A new PCR based method for the generation of nested deletions.
Nucleic acids research 1993;21(17):4143-6.
1993: C F Boerkoel; Mark J Federspiel; DW Salter; W Payne; L B Crittenden; Hsing-Jien Kung; Stephen H Hughes
A new defective retroviral vector system based on the Bryan strain of Rous sarcoma virus.
Virology 1993;195(2):669-79.
1993: Amnon Hizi; R Tal; M Shaharabany; M J Currens; Michael R Boyd; Stephen H Hughes; James B McMahon
Specific inhibition of the reverse transcriptase of human immunodeficiency virus type 1 and the chimeric enzymes of human immunodeficiency virus type 1 and type 2 by nonnucleoside inhibitors.
Antimicrobial agents and chemotherapy 1993;37(5):1037-42.
1993: Paul L Boyer; M J Currens; James B McMahon; Michael R Boyd; Stephen H Hughes
Analysis of nonnucleoside drug-resistant variants of human immunodeficiency virus type 1 reverse transcriptase.
Journal of virology 1993;67(4):2412-20.
1993: Paul L Boyer; Clemencia Colmenares; Ed Stavnezer; Stephen H Hughes
Sequence and biological activity of chicken snoN cDNA clones.
Oncogene 1993;8(2):457-66.
1992: Paul L Boyer; A L Ferris; Stephen H Hughes
Mutational analysis of the fingers domain of human immunodeficiency virus type 1 reverse transcriptase.
Journal of virology 1992;66(12):7533-7.
1992: M Kotler; G Arad; Stephen H Hughes
Human immunodeficiency virus type 1 gag-protease fusion proteins are enzymatically active.
Journal of virology 1992;66(11):6781-3.
1992: Yoel Kashman; Kirk R Gustafson; RW Fuller; John H Cardellina; James B McMahon; M J Currens; Robert W Buckheit; Stephen H Hughes; Gordon M Cragg; Michael R Boyd
The calanolides, a novel HIV-inhibitory class of coumarin derivatives from the tropical rainforest tree, Calophyllum lanigerum.
Journal of medicinal chemistry 1992;35(15):2735-43.
1992: Shoshana Loya; R Tal; Stephen H Hughes; Amnon Hizi
The effects of cysteine mutations on the catalytic activities of the reverse transcriptase of human immunodeficiency virus type-1.
The Journal of biological chemistry 1992;267(20):13879-83.
1992: C J Petropoulos; W Payne; DW Salter; Stephen H Hughes
Appropriate in vivo expression of a muscle-specific promoter by using avian retroviral vectors for gene transfer [corrected].
Journal of virology 1992;66(6):3391-7.
1992: Paul L Boyer; A L Ferris; Stephen H Hughes
Cassette mutagenesis of the reverse transcriptase of human immunodeficiency virus type 1.
Journal of virology 1992;66(2):1031-9.
1992: Amnon Hizi; M Shaharabany; R Tal; Stephen H Hughes
The effects of cysteine mutations on the reverse transcriptases of human immunodeficiency virus types 1 and 2.
The Journal of biological chemistry 1992;267(2):1293-7.
1992: I Givol; J J Greenhouse; Stephen H Hughes; Donald l Ewert
Retroviruses that express different ras mutants cause different types of tumors in chickens.
Oncogene 1992;7(1):141-6.
1992: J M Whitcomb; Stephen H Hughes
Retroviral reverse transcription and integration: progress and problems.
Annual review of cell biology 1992;8():275-306.
1991: A Jacobo-Molina; Arthur D Clark; R L Williams; R G Nanni; P Clark; A L Ferris; Stephen H Hughes; Eddy Arnold
Crystals of a ternary complex of human immunodeficiency virus type 1 reverse transcriptase with a monoclonal antibody Fab fragment and double-stranded DNA diffract x-rays to 3.5-A resolution.
Proceedings of the National Academy of Sciences of the United States of America 1991;88(23):10895-9.
1991: Clemencia Colmenares; P Sutrave; Stephen H Hughes; Ed Stavnezer
Activation of the c-ski oncogene by overexpression.
Journal of virology 1991;65(9):4929-35.
1991: S Forry-Schaudies; Stephen H Hughes
The chicken tropomyosin 1 gene generates nine mRNAs by alternative splicing.
The Journal of biological chemistry 1991;266(21):13821-7.
1991: J M Whitcomb; Stephen H Hughes
The sequence of human immunodeficiency virus type 2 circle junction suggests that integration protein cleaves the ends of linear DNA asymmetrically.
Journal of virology 1991;65(7):3906-10.
1991: C J Petropoulos; Stephen H Hughes
Replication-competent retrovirus vectors for the transfer and expression of gene cassettes in avian cells.
Journal of virology 1991;65(7):3728-37.
1991: P Sutrave; Stephen H Hughes
The ski oncogene.
Oncogene 1991;6(3):353-6.
1991: L Hernandez; C J Petropoulos; Stephen H Hughes; W Lijinsky
DNA methylation and oncogene expression in methapyrilene-induced rat liver tumors and in treated hepatocytes in culture.
Molecular carcinogenesis 1991;4(3):203-9.
1991: Mark J Federspiel; L B Crittenden; L P Provencher; Stephen H Hughes
Experimentally introduced defective endogenous proviruses are highly expressed in chickens.
Journal of virology 1991;65(1):313-9.
1991: Amnon Hizi; R Tal; Stephen H Hughes
Mutational analysis of the DNA polymerase and ribonuclease H activities of human immunodeficiency virus type 2 reverse transcriptase expressed in Escherichia coli.
Virology 1991;180(1):339-46.
1990: S Forry-Schaudies; C E Gruber; Stephen H Hughes
Chicken cardiac tropomyosin and a low-molecular-weight nonmuscle tropomyosin are related by alternative splicing.
Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research 1990;1(10):473-81.
1990: J M Whitcomb; R Kumar; Stephen H Hughes
Sequence of the circle junction of human immunodeficiency virus type 1: implications for reverse transcription and integration.
Journal of virology 1990;64(10):4903-6.
1990: P Sutrave; A M Kelly; Stephen H Hughes
ski can cause selective growth of skeletal muscle in transgenic mice.
Genes & development 1990;4(9):1462-72.
1990: Amnon Hizi; Stephen H Hughes; M Shaharabany
Mutational analysis of the ribonuclease H activity of human immunodeficiency virus 1 reverse transcriptase.
Virology 1990;175(2):575-80.
1990: A L Ferris; Amnon Hizi; S D Showalter; S Pichuantes; L Babe; Charles S Craik; Stephen H Hughes
Immunologic and proteolytic analysis of HIV-1 reverse transcriptase structure.
Virology 1990;175(2):456-64.
1990: S Forry-Schaudies; Nita Maihle; Stephen H Hughes
Generation of skeletal, smooth and low molecular weight non-muscle tropomyosin isoforms from the chicken tropomyosin 1 gene.
Journal of molecular biology 1990;211(2):321-30.
1990: Stephen H Hughes; C J Petropoulos; M J Federspiel; P Sutrave; S Forry-Schaudies; J A Bradac
Vectors and genes for improvement of animal strains.
Journal of reproduction and fertility. Supplement 1990;41():39-49.
1989: D Raveh; Stephen H Hughes; B K Shafer; Jeffrey N Strathern
Analysis of the HO-cleaved MAT DNA intermediate generated during the mating type switch in the yeast Saccharomyces cerevisiae.
Molecular & general genetics : MGG 1989;220(1):33-42.
1989: Mark J Federspiel; L B Crittenden; Stephen H Hughes
Expression of avian reticuloendotheliosis virus envelope confers host resistance.
Virology 1989;173(1):167-77.
1989: P Sutrave; Stephen H Hughes
Isolation and characterization of three distinct cDNAs for the chicken c-ski gene.
Molecular and cellular biology 1989;9(9):4046-51.
1989: Amnon Hizi; A Barber; Stephen H Hughes
Effects of small insertions on the RNA-dependent DNA polymerase activity of HIV-1 reverse transcriptase.
Virology 1989;170(1):326-9.
1989: J A Bradac; C E Gruber; S Forry-Schaudies; Stephen H Hughes
Isolation and characterization of related cDNA clones encoding skeletal muscle beta-tropomyosin and a low-molecular-weight nonmuscle tropomyosin isoform.
Molecular and cellular biology 1989;9(1):185-92.
1988: Amnon Hizi; Stephen H Hughes
Expression of the Moloney murine leukemia virus and human immunodeficiency virus integration proteins in Escherichia coli.
Virology 1988;167(2):634-8.
1988: J J Greenhouse; C J Petropoulos; L B Crittenden; Stephen H Hughes
Helper-independent retrovirus vectors with Rous-associated virus type O long terminal repeats.
Journal of virology 1988;62(12):4809-12.
1988: M Nakazawa; T Aida; W V Everson; Matthew A Gonda; Stephen H Hughes; Winston W-Y Kao
Structure of the gene encoding the beta-subunit of chicken prolyl 4-hydroxylase.
Gene 1988;71(2):451-60.
1988: A Hizi; C McGill; Stephen H Hughes
Expression of soluble, enzymatically active, human immunodeficiency virus reverse transcriptase in Escherichia coli and analysis of mutants.
Proceedings of the National Academy of Sciences of the United States of America 1988;85(4):1218-22.
1988: Winston W-Y Kao; M Nakazawa; T Aida; W V Everson; Candace W-C Kao; J M Seyer; Stephen H Hughes
Isolation of cDNA clones and genomic DNA clones of beta-subunit of chicken prolyl 4-hydroxylase.
Connective tissue research 1988;18(3):157-74.
1987: Stephen H Hughes; J J Greenhouse; C J Petropoulos; P Sutrave
Adaptor plasmids simplify the insertion of foreign DNA into helper-independent retroviral vectors.
Journal of virology 1987;61(10):3004-12.
1987: DW Salter; E J Smith; Stephen H Hughes; S E Wright; L B Crittenden
Transgenic chickens: insertion of retroviral genes into the chicken germ line.
Virology 1987;157(1):236-40.
1986: Stephen H Hughes; E Kosik; A M Fadly; DW Salter; L B Crittenden
Design of retroviral vectors for the insertion of foreign deoxyribonucleic acid sequences into the avian germ line.
Poultry science 1986;65(8):1459-67.
1986: DW Salter; E J Smith; Stephen H Hughes; S E Wright; A M Fadly; R L Witter; L B Crittenden
Gene insertion into the chicken germ line by retroviruses.
Poultry science 1986;65(8):1445-58.